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free radical scavenging action of bio-catalyzer alpha.rho no.11 (bio-normalyzer) and its by-product.bio-catalyzer alpha.rho no.11 (bio-normalyzer) and its by-product are natural health products made by yeast fermentation of glucose, carica papaya linn., pennisetum pupureum schum., and sechium edule swartz. their effects on free radicals were examined by electron spin resonance spectrometry using spin trapping agent 5,5-dimethyl-1-pyrroline-1-oxide (dmpo). it was observed that both bio-catalyzer and its by-product scavenged 95% of dmpo-oh spin adducts (89 x 10(15) spins/ml) generated by feso4-h ...19911665836
[diuretic effect of chayote juice (sechium edule) in rats]. 19846701355
isolation of cryptococcus neoformans var. neoformans from bird droppings, fruits and vegetables in mexico city.the presence of cryptococcus neoformans in various natural sources, such as bird droppings, fruits and vegetables, was investigated. a total of 711 samples were analyzed; c. neoformans var. neoformans was isolated from seven out of 74 bird droppings (9.5%), with parrots as one of the most significant sources. fruits were positive in 9.5% of the 169 samples studied, specially citrus fruits, particularly grapefruit, in which the highest frequency was found. from the 468 vegetable samples, only 20 ...19957617014
sechiumin, a ribosome-inactivating protein from the edible gourd, sechium edule swartz--purification, characterization, molecular cloning and expression.a new ribosome-inactivating protein (rip), sechiumin, was purified from the seeds of edible gourd, sechium edule swartz by gel-filtration and ion-exchange chromatography, with an apparent relative molecular mass of 27 kda. it inhibits the protein synthesis of rabbit reticulocyte lysate strongly with a concentration causing 50% inhibition (ic50) of 0.7 nm, but has a much lower inhibitory effect on intact hela cells, with an ic50 of 5000 nm. sechiumin has a highly specific rna n-glycosidase activi ...19989716381
the antihypertensive effects of the jamaican cho-cho (sechium edule).the experiments reported in this study constitute a preliminary investigation into the possible hypotensive effect of the jamaican cho-cho (sechium edule). experiments were conducted in a random and blind fashion on two sub species of sechium edule. both the pulp and the peel were examined for hypotensive activity. water-soluble extracts were prepared from these components of the fruit and injected into anaesthetised rats. various cardiovascular parameters were measured including heart rate, mea ...200010786447
improvement of hemorheological abnormalities in alcoholics by an oral antioxidant.it has been shown that alcohol impairs erythrocyte (red blood cell) membrane fluidity and lipid composition. the aim of this study was to test the effect of a novel acid-resistant antioxidant on the hemorrheology in alcoholics.200411379344
evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants.this study evaluates the toxic, mutagenic and antimutagenic effects of emerging edible plants that are consumed as new leafy vegetables in taiwan. among eight plant extracts, only the extracts of sol (solanum nigrum l.) showed cytotoxicity to salmonella typhimurium ta100 in the absence of s9 mix. the toxicity of extracts from different parts of the sol plant, such as leaf and stem, immature fruit and mature fruit, towards s. typhimurium ta100 and human lymphocytes was also assayed. the immature ...200111527563
effect of a chayotte (sechium edule) extract on the labeling of red blood cells and plasma proteins with technetium-99m: in vitro and in vivo studies.sechium edule (chayotte) is used as food or as medication in popular medicine. the labeling of blood elements with technetium-99m (99mtc) has been altered by drugs (synthetic and natural). some authors have reported biological effects concerning the chayotte. we have evaluated the influence of chayotte extracts (macerated and infusion) on the labeling of blood elements with 99mtc. in vitro study, blood was incubated with the extracts, (6.25, 12.5, 25, 50 and 100% v/v). in in vivo study, the anim ...200212619970
determination and reoxidation of the disulfide bridges of a squash-type trypsin inhibitor from sechium edule seeds.the determination of the disulfide pairings of seti-ii, a trypsin inhibitor isolated from sechium edule, is described herein. the inhibitor contains 31 amino acid residues per mol, 6 of which are cysteine. forty-five nmol (160 microg) of seti-ii was hydrolyzed with 20 microg thermolysin for 48 hr at 45 degrees c, and peptides were separated by reverse phase high performance liquid chromatography (rp-hplc). the major products were identified by amino acid composition, edman degradation, and on th ...200415328886
study of flavonoids of sechium edule (jacq) swartz (cucurbitaceae) different edible organs by liquid chromatography photodiode array mass spectrometry.a liquid chromatography-mass spectrometry (lc-ms)-based method was developed for the characterization of flavonoids from sechium edule (jacq) swartz (cucurbitaceae) edible organs, a plant cultivated since pre-colombian times in mexico where the fruit is called chayote. chayote is used for human consumption in many countries; in addition to the fruits, stems, leaves and the tuberous part of the roots are also eaten. eight flavonoids, including three c-glycosyl and five o-glycosyl flavones, were d ...200415479015
low molecular weight squash trypsin inhibitors from sechium edule seeds.nine chromatographic components containing trypsin inhibitor activity were isolated from sechium edule seeds by acetone fractionation, gel filtration, affinity chromatography and rp-hplc in an overall yield of 46% of activity and 0.05% of protein. the components obtained with highest yield of total activity and highest specific activity were sequenced by edman degradation and their molecular masses determined by mass spectrometry. the inhibitors contained 31, 32 and 27 residues per molecule and ...200616406091
biological effects of a chayotte extract in wistar rats with induced diabetes: a radiopharmaceutically analysis.the present research evaluated the influence of a chayotte (sechium edule) extract (macerated) on the bioavailability of 99tco4na as well as in the mass of the organs. in this study, in the biodistribution analysis, the 99mtco4na was administrated into female wistar rats (diabetes and no diabetes induced) which had drunk or not the extract for 7 days. after 10 min, animals were sacrificed, the organs were isolated, the radioactivity determined in a well counter and the percentages of radioactivi ...200719069536
effects of a chayotte (sechium edule) extract (macerated) on the biochemistry of blood of wistar rats and on the action against the stannous chloride effect.the use of natural products as medicines has been growing in the entire world. there are concerns that these products may contain potentially toxic ingredients and contaminants such as heavy metals. the labeling of blood constituents with technetium-99m has been influenced by the presence of natural extracts. we evaluated the influence of a chayotte (sechium edule) extract (100% v/v macerated) on the labeling of blood elements with 99mtc. the animals were treated with the extract during 15 days. ...200719069873
design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.the aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of sechium edule fluid extract and to obtain a pharmaceutical formulation with them. the extract exhibited antimicrobial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, serratia marcescens, morganella morganii, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia, candida spp. and aspergillus spp. isolated fr ...200919477252
vasoactive and antioxidant activities of plants used in mexican traditional medicine for the treatment of cardiovascular diseases.this study demonstrated that the aqueous extracts of plants employed in mexican traditional medicine for the treatment of cardiovascular diseases are able to modify the tone of arterial smooth muscle. agastache mexicana (kunth) lint & epling (labiatae), chenopodium murale l. (chenopodiaceae), chirantodendron pentadactylon larreat (sterculiaceae), dracocephalum moldavica l. (labiatae), psittacanthus calyculatus g. don (loranthaceae), prunus serotina ssp. capuli (cav. ex spreng) mcvaugh (rosaceae) ...201020645769
pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus colletotrichum gloeosporioides (phyllachoraceae) from the state of pernambuco, brazil.onion anthracnose, caused by colletotrichum gloeosporioides, is one of the main diseases of onions in the state of pernambuco. we examined the pathogenicity of 15 c. gloeosporioides strains and analyzed their genetic variability using rapds and internal transcribed spacers (its) of the rdna region. ten of the strains were obtained from substrates and hosts other than onion, including chayote (sechium edule), guava (psidium guajava), pomegranate (punica granatum), water from the capibaribe river, ...201121365546
taxonomic significance of trichomes micromorphology in cucurbits.studies on trichomes micromorphology using scanning electron microscope (sem) were undertaken in 23 species with one variety under 13 genera of the family cucurbitaceae (viz., benincasa hispida (thunb.) cogn., citrullus lanatus (thunb.) matsum. & nakai, cucumis melo var. agrestis naudin, cucumis sativus l., diplocyclos palmatus (l.) c. jeffrey, edgaria dargeelingensis c.b. clarke, gynostemma burmanicum king ex chakr., gynostemma pentaphyllum (thunb.) makino, gynostemma pubescens (gagnep.) c.y. w ...201123961108
biocontrol efficacy and plant growth promoting activity of bacillus altitudinis isolated from darjeeling hills, india.a total of 18 bacterial isolates were obtained from the rhizosphere of sechium edule growing in the lower foothills of darjeeling, india. the bacterial isolates were tested for pgpr traits in vitro such as phosphate solubilization, hcn, siderophore, iaa, chitinase, protease production as well as inhibition of pthytopathogens. of all the bacterial isolates, one bacterium designated as brhs/s-73 was found to possess all the tested characters which was identified on the basis of 16s rrna gene seque ...201523996212
antioxidant and α-glucosidase inhibitory activities of cucurbit fruit vegetables and identification of active and major constituents from phenolic-rich extracts of lagenaria siceraria and sechium edule.antioxidant and α-glucosidase activities and total phenolic contents (tpc) in sequential extracts of dried pulps from seven cucurbit fruit vegetables were determined for the first time. the highest tpc and metal chelating activity were obtained from the chloroform extracts of luffa acutangula (28.04 ± 0.37 mg gae/g extract) and benincasa hispida (ec50 = 0.44 ± 0.03 mg/ml), respectively. the ethyl acetate extract of sechium edule showed the highest 1,1-diphenyl-2-picrylhydrazyl (dpph) free radica ...201324059845
antioxidant activity in extracts of 27 indigenous taiwanese vegetables.the objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of taiwan's indigenous vegetables. lycium chinense (lc), lactuca indica (li), and perilla ocymoides (po) contained abundant quercetin (que), while artemisia lactiflora (al) and gynura bicolor (gb) were rich in morin and kaempferol, respectively. additionally, nymphoides cristata (nc) and sechium edule (se)-yellow had significantly higher levels of myricetin (myr) than other tested samples. cyanidin (cya ...201424858497
occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica.leaf samples of solanum lycopersicum, capsicum annuum, cucurbita moschata, cucurbita pepo, sechium edule and erythrina spp. were collected. all samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. a sequence of ∼1,100 bp was obtained from the genomic component dna-a of 14 samples. in addition, one sequence of ∼580 bp corresponding to the coat protein (av1) was obtained from a chayote (s. edule) leaf sample. the presence of squash yellow mild mottle viru ...201325288955
polysaccharide composition of raw and cooked chayote (sechium edule sw.) fruits and tuberous roots.chayote is a multipurpose table vegetable widely consumed in latin america countries. chayote fruits, leaves and tuberous roots contain complex carbohydrates as dietary fiber and starch, vitamins and minerals. the complex polysaccharides (cell walls and starch) were analyzed in the black and green varieties of chayote fruits as well as in green chayote tuberous root before and after a controlled cooking process to assess changes in their composition and structure. the monosaccharide composition ...201526076612
biology of anastrepha grandis (diptera: tephritidae) in different cucurbits.anastrepha grandis (macquart) (diptera: tephritidae) is one of the main pests of cucurbits in brazil. losses occur due to the damage caused to the fruits and the embargo on exports, as a. grandis is considered a quarantine pest in countries that import brazilian cucurbits. this study aimed to evaluate the development of a. grandis in hosts of the cucurbitaceae family. the hosts used were stem squash (cucurbita pepo l.), squash (cucurbita moschata duchesne), chayote [sechium edule (jacq.) swartz] ...201526470226
purification and partial characterization of β-glucosidase in chayote (sechium edule).β-glucosidase (ec 3.2.1.21) is a prominent member of the gh1 family of glycoside hydrolases. the properties of this β-glucosidase appear to include resistance to temperature, urea, and iodoacetamide, and it is activated by 2-me, similar to other members. β-glucosidase from chayote (sechium edule) was purified by ionic-interchange chromatography and molecular exclusion chromatography. peptides detected by lc-esi-ms/ms were compared with other β-glucosidases using the blast program. this enzyme is ...201526512637
purification and partial characterization of β-glucosidase in chayote (sechium edule).β-glucosidase (ec 3.2.1.21) is a prominent member of the gh1 family of glycoside hydrolases. the properties of this β-glucosidase appear to include resistance to temperature, urea, and iodoacetamide, and it is activated by 2-me, similar to other members. β-glucosidase from chayote (sechium edule) was purified by ionic-interchange chromatography and molecular exclusion chromatography. peptides detected by lc-esi-ms/ms were compared with other β-glucosidases using the blast program. this enzyme is ...201526556317
purification and partial characterization of β-glucosidase in chayote (sechium edule).β-glucosidase (ec 3.2.1.21) is a prominent member of the gh1 family of glycoside hydrolases. the properties of this β-glucosidase appear to include resistance to temperature, urea, and iodoacetamide, and it is activated by 2-me, similar to other members. β-glucosidase from chayote (sechium edule) was purified by ionic-interchange chromatography and molecular exclusion chromatography. peptides detected by lc-esi-ms/ms were compared with other β-glucosidases using the blast program. this enzyme is ...201526561793
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