Publications

jasmonic acid-induced volatiles of brassica oleracea attract parasitoids: effects of time and dose, and comparison with induction by herbivores.	caterpillar feeding induces direct and indirect defences in brassicaceous plants. this study focused on the role of the octadecanoid pathway in induced indirect defence in brassica oleracea. the effect of induction by exogenous application of jasmonic acid (ja) on the responses of brussels sprouts plants and on host-location behaviour of associated parasitoid wasps was studied. feeding by the biting-chewing herbivores pieris rapae and plutella xylostella resulted in significantly increased endog ...	2009	19451186
comparing induction at an early and late step in signal transduction mediating indirect defence in brassica oleracea.	the induction of plant defences involves a sequence of steps along a signal transduction pathway, varying in time course. in this study, the effects of induction of an early and a later step in plant defence signal transduction on plant volatile emission and parasitoid attraction are compared. ion channel-forming peptides represent a class of inducers that induce an early step in signal transduction. alamethicin (ala) is an ion channel-forming peptide mixture from the fungus trichoderma viride t ...	2009	19454598
use of single cell gel electrophoresis assays for the detection of dna-protective effects of dietary factors in humans: recent results and trends.	this article summarises the results of human dietary intervention trials employing the comet assay (single cell gel electrophoresis, scge), which have been published in the last few years (i.e., between 2005 and 2008) and describes new trends and developments as well as current problems concerning the design of intervention trials and the interpretation of the results. most new studies were carried out with complex plant derived foods and juices; only a few were conducted with individual food co ...	2009	18755290
galactolipids as potential health promoting compounds in vegetable foods.	galactolipids are a class of compounds widely found in the plant kingdom, including edible plants, and are an important part of the cell membranes. galactolipids in plants consists mainly of monogalactosyldiacylglycerols and digalactosyldiacylglycerols containing one or two saturated and/or unsaturated fatty acids linked to the glycerol moiety. several galactolipids have been shown to possess in vitro and/or in vivo anti-tumor promoting activity and anti-inflammatory activity. recently, it has b ...	2009	20653526
anti-aphrodisiac compounds of male butterflies increase the risk of egg parasitoid attack by inducing plant synomone production.	during mating in many butterfly species, males transfer spermatophores that contain anti-aphrodisiacs to females that repel conspecific males. for example, males of the large cabbage white, pieris brassicae (lepidoptera: pieridae), transfer the anti-aphrodisiac, benzyl cyanide (bc) to females. accessory reproductive gland (arg) secretion of a mated female p. brassicae that is deposited with an egg clutch contains traces of bc, inducing brussels sprouts plants (brassica oleracea var. gemmifera) t ...	2009	19949841
a novel method for efficient and abundant production of phytophthora brassicae zoospores on brussels sprout leaf discs.	phytophthora species are notorious oomycete pathogens that cause diseases on a wide range of plants. our understanding how these pathogens are able to infect their host plants will benefit greatly from information obtained from model systems representative for plant-phytophthora interactions. one attractive model system is the interaction between arabidopsis and phytophthora brassicae. under laboratory conditions, arabidopsis can be easily infected with mycelial plugs as inoculum. in the disease ...	2009	19698127
consequences of constitutive and induced variation in plant nutritional quality for immune defence of a herbivore against parasitism.	the mechanisms through which trophic interactions between species are indirectly mediated by distant members in a food web have received increasing attention in the field of ecology of multitrophic interactions. scarcely studied aspects include the effects of varying plant chemistry on herbivore immune defences against parasitoids. we investigated the effects of constitutive and herbivore-induced variation in the nutritional quality of wild and cultivated populations of cabbage (brassica olerace ...	2009	19271243
cruciferous plants: phytochemical toxicity versus cancer chemoprotection.	the cruciferae (also known as the brassicaceae) are the family of plants that include the various familiar members of the species brassica oleracea (e.g., broccoli, cabbage, cauliflower, kale, brussels sprouts) as well as many other plants that are widely consumed in various parts of the world. forage and root brassicas are widely used as winter feeds for cattle and sheep. a striking and characteristic chemical property of cruciferous plants is their high content of glucosinolates (more than 120 ...	2009	20205629
glucosinolates in brassica vegetables: the influence of the food supply chain on intake, bioavailability and human health.	glucosinolates (glss) are found in brassica vegetables. examples of these sources include cabbage, brussels sprouts, broccoli, cauliflower and various root vegetables (e.g. radish and turnip). a number of epidemiological studies have identified an inverse association between consumption of these vegetables and the risk of colon and rectal cancer. animal studies have shown changes in enzyme activities and dna damage resulting from consumption of brassica vegetables or isothiocyanates, the breakdo ...	2009	19035553
foods high in fiber and phytobezoar formation.	high-fiber diets are being recommended by government agencies, cancer institutes, and manufacturers of high-fiber foods. although this recommendation is appropriate for the majority of clients, some persons are prone to form phytobezoars and should not add certain kinds of fiber to the diet. the phytobezoar is a compact mass of fibers, skins, seeds, leaves, roots, or stems of plants that collects in the stomach or small intestine. other food particles, such as fats, crystals, granules, fibers, a ...	2009	2824590
forward and reverse genetics of rapid-cycling brassica oleracea.	seeds of rapid-cycling brassica oleracea were mutagenized with the chemical mutagen, ethylmethane sulfonate. the reverse genetics technique, tilling, was used on a sample population of 1,000 plants, to determine the mutation profile. the spectrum and frequency of mutations induced by ethylmethane sulfonate was similar to that seen in other diploid species such as arabidopsis thaliana. these data indicate that the mutagenesis was effective and demonstrate that tilling represents an efficient reve ...	2009	19132334
dietary intake of individual glucosinolates in participants of the epic-heidelberg cohort study.	to compile a database on content of individual glucosinolates in food and to describe the dietary intake of individual glucosinolates in a german population.	2009	19295191
characterization and quantification of flavonoids and hydroxycinnamic acids in curly kale (brassica oleracea l. convar. acephala var. sabellica) by hplc-dad-esi-msn.	kale is a leafy green vegetable belonging to the brassicaceae family, a group of vegetables including cabbage, broccoli, cauliflower, and brussels sprouts, with a high content of health-promoting phytochemicals. the flavonoids and hydroxycinammic acids of curly kale ( brassica oleracea l. ssp. oleracea convar. acephala (dc.) alef. var. sabellica l.), a variety of kale, were characterized and identified primarily through hplc-dad-esi-ms(n) analysis. thirty-two phenolic compounds including glycosi ...	2009	19253943
inhibition of lipoxygenase affects induction of both direct and indirect plant defences against herbivorous insects.	herbivore-induced plant defences influence the behaviour of insects associated with the plant. for biting-chewing herbivores the octadecanoid signal-transduction pathway has been suggested to play a key role in induced plant defence. to test this hypothesis in our plant-herbivore-parasitoid tritrophic system, we used phenidone, an inhibitor of the enzyme lipoxygenase (lox), that catalyses the initial step in the octadecanoid pathway. phenidone treatment of brussels sprouts plants reduced the acc ...	2010	19806367
effect of spray application technology on the biological control of aphids in brussels sprouts.	a field trial was carried out to evaluate different application techniques for crop penetration and biological efficacy of aphid control in brussels sprouts. six different application techniques were tested at a pressure of 4.0 bar in a field trial in 3 parallels at the provincial vegetable research centre in kruishoutem (pcg): a standard iso 02 flat fan nozzle (at 200 l/ha), an iso 04 twin air inclusion nozzle (at 800 l/ha), an iso 03 drift reducing nozzle, an air injection nozzle (airjet,) dro ...	2010	21542477
the table maneuver: a procedure used with success in four cases of unconscious choking older subjects.	confronted with an unconscious choking victim, it is recommended to call an ambulance and start mouth-to-mouth and cardiopulmonary resuscitation (cpr). we describe a procedure called the "table maneuver" that was successful in relieving foreign-body airway obstruction in 4 cases of choking in unconscious elderly patients over a 6-year period in the same long-term care facility.	2010	20870197
quantitative determination of glucoraphanin in brassica vegetables by micellar electrokinetic capillary chromatography.	glucoraphanin, a glucosinolate, is found naturally in plants and is present in relatively high concentrations in broccoli. glucosinolates have received much attention as studies have indicated that a diet rich in them may provide some protection from certain cancers. a micellar electrokinetic chromatography (mekc) method using sodium cholate as the micellar phase has been developed to quantify for glucoraphanin in broccoli (seeds and florets) and brussels sprouts. the glucoraphanin peak elutes j ...	2010	20172104
broccoli, pten deletion and prostate cancer: where is the link?	the concept that vegetables and fruits are relevant sources of cancer-preventive substances is strongly supported by population studies. among others, cruciferous vegetables like broccoli, cabbage, cauliflower and brussels sprouts are thought to affect the development of various types of cancers and especially prostate tumors. yet, the identification of the molecular mechanisms by which the 'active' compounds contained in these vegetables mediate their anticancer activity has historically lagged ...	2010	21122140
protective effect of sulforaphane against oxidative stress: recent advances.	sulforaphane [1-isothiocyanate-(4r)-(methylsulfinyl)butane] is a natural dietary isothiocyanate produced by the enzymatic action of the myrosinase on glucopharanin, a 4-methylsulfinylbutyl glucosinolate contained in cruciferous vegetables of the genus brassica such as broccoli, brussel sprouts, and cabbage. studies on this compound is increasing because its anticarcinogenic and cytoprotective properties in several in vivo experimental paradigms associated with oxidative stress such as focal cere ...	2010	21129940
induction of detoxification enzymes by feeding unblanched brussels sprouts containing active myrosinase to mice for 2 wk.	in cruciferous vegetables, myrosinase metabolizes the relatively inactive glucosinolates into isothiocyanates and other products that have the ability to increase detoxification enzyme expression. thus, maintaining myrosinase activity during food preparation may be critical to receiving the maximum benefit of consumption of brussels sprouts or other cruciferous vegetables. to test the importance of maintaining myrosinase activity for maximizing bioactivity, experimental diets containing 20% unbl ...	2010	20722931
multidirectional time-dependent effect of sinigrin and allyl isothiocyanate on metabolic parameters in rats.	sinigrin (sin) and allyl isothiocyanate (aitc) are compounds found in high concentrations in brassica family vegetables, especially in brussels sprouts. recently, they have been used as a nutrition supplement for their preventive and medicinal effect on some types of cancer and other diseases. in this research, nutritional significance of parent glucosinolate sinigrin 50 μmol/kg b. w./day and its degradation product allyl isothiocyanate 25 μmol/kg b. w./day and 50 μmol/kg b. w./day was studied b ...	2010	20809411
carotenoid profiles in provitamin a-containing fruits and vegetables affect the bioefficacy in mongolian gerbils.	fruits and vegetables are rich sources of provitamin a carotenoids. we evaluated the vitamin a (va) bioefficacy of a whole foods supplement (wfs) and its constituent green vegetables (study 1) and a variety of fruits with varying ratios of provitamin a carotenoids (study 2) in va-depleted mongolian gerbils (n = 77/study). after feeding a va-deficient diet for 4 and 6 weeks in studies 1 and 2, respectively, customized diets, equalized for va, were fed for 4 and 3 weeks, respectively. both studies ...	2010	20558838
effect of kaempferol on lipid peroxidation and antioxidant status in 1,2-dimethyl hydrazine induced colorectal carcinoma in rats.	colorectal cancer, a common cause of cancer related deaths in both sexes in western population is often due to persistent oxidative stress leading to dna damage. antioxidants scavenge free radicals and inhibit neoplastic process. kaempferol, a flavonol widely distributed in tea, broccoli, grape fruit, brussels sprouts and apple, is claimed to have chemopreventive action in colon cancer. the aim of our study was to evaluate the effect of kaempferol on tissue lipid peroxidation and antioxidant sta ...	2010	21172346
heat treatment of brussels sprouts retains their ability to induce detoxification enzyme expression in vitro and in vivo.	the bioactive metabolites of glucosinolates, such as isothiocyanates, contained in cruciferous vegetables have been shown to reduce the risk of cancers through the induction of detoxification enzymes. however, cruciferous vegetables are commonly processed before consumption, significantly altering the phytochemical composition of these vegetables. compared to freeze-dried brussels sprouts, oven-dried brussels sprouts contain low concentrations of glucosinolates (22.14 and 0.85 μmol/g, respective ...	2011	21535814
identification of human urinary biomarkers of cruciferous vegetable consumption by metabonomic profiling.	consumption of cruciferous vegetables (cvs) is inversely correlated to many human diseases including cancer (breast, lung, and bladder), diabetes, cardiovascular and neurological disease. presently, there are no readily measureable biomarkers of cv consumption and intake of cvs has relied on dietary recall. here, biomarkers of cv intake were identified in the urine of 20 healthy caucasian adult males using 1h-nmr spectroscopy with multivariate statistical modeling. the study was separated into t ...	2011	21770373
anti-proliferative activity and chemoprotective effects towards dna oxidative damage of fresh and cooked brassicaceae.	epidemiological evidence shows that regular consumption of brassicaceae is associated with a reduced risk of cancer and heart disease. cruciferous species are usually processed before eating and the real impact of cooking practices on their bioactive properties is not fully understood. we have evaluated the effect of common cooking practices (boiling, microwaving, and steaming) on the biological activities of broccoli, cauliflower and brussels sprouts. anti-proliferative and chemoprotective effe ...	2011	22088277
Small Bowel Obstruction from Potato and Broccoli Phytobezoar Mimicking Mesenteric Ischemia.	BACKGROUND: Bezoars are concretions of undigested foreign material that form in the gastrointestinal tract. Rare in humans, they are nonetheless a well-documented cause of intraluminal bowel obstruction. OBJECTIVES: The objectives of this case report include describing an unusual presentation of small bowel obstruction due to phytobezoar, which mimicked mesenteric ischemia, and highlighting the risk factors, presentation, and management of bezoars, in addition to covering historical beliefs rega ...	2011	22051841
phenolic composition, antioxidant capacity and antibacterial activity of selected irish brassica vegetables.	vegetables belonging to the brassicaceae family are rich in polyphenols, flavonoids and glucosinolates, and their hydrolysis products, which may have antibacterial, antioxidant and anticancer properties. in the present study, phenolic composition, antibacterial activity and antioxidant capacity of selected brassica vegetables, including york cabbage, brussels sprouts, broccoli and white cabbage were evaluated after extraction with aqueous methanol. results obtained showed that york cabbage extra ...	2011	21941903
target protein interactions of indole-3-carbinol and the highly potent derivative 1-benzyl-i3c with the c-terminal domain of human elastase uncouples cell cycle arrest from apoptotic signaling.	elastase is the only currently identified target protein for indole-3-carbinol (i3c), a naturally occurring hydrolysis product of glucobrassicin in cruciferous vegetables such as broccoli, cabbage, and brussels sprouts that induces a cell cycle arrest and apoptosis of human breast cancer cells. in vitro elastase enzymatic assays demonstrated that i3c and at lower concentrations its more potent derivative 1-benzyl-indole-3-carbinol (1-benzyl-i3c) act as non-competitive allosteric inhibitors of el ...	2011	22012859
attenuation of multi-targeted proliferation-linked signaling by 3,3'-diindolylmethane (dim): from bench to clinic.	emerging evidence provide credible support in favor of the potential role of bioactive products derived from ingesting cruciferous vegetables such as broccoli, brussel sprouts, cauliflower and cabbage. among many compounds, 3,3'-diindolylmethane (dim) is generated in the acidic environment of the stomach following dimerization of indole-3-carbinol (i3c) monomers present in these classes of vegetables. both i3c and dim have been investigated for their use in preventing, inhibiting, and reversing ...	2011	21703360
indole-3-carbinol downregulation of telomerase gene expression requires the inhibition of estrogen receptor-alpha and sp1 transcription factor interactions within the htert promoter and mediates the g1 cell cycle arrest of human breast cancer cells.	indole-3-carbinol (i3c), a naturally occurring hydrolysis product of glucobrassicin from cruciferous vegetables such as broccoli, cabbage and brussels sprouts, is an anticancer phytochemical that triggers complementary sets of antiproliferative pathways to induce a cell cycle arrest of estrogen-responsive mcf7 breast cancer cells. i3c strongly downregulated transcript expression of the catalytic subunit of the human telomerase (htert) gene, which correlated with the dose-dependent indole-mediate ...	2011	21693539
a rapid quantitative determination of phenolic acids in brassica oleracea by capillary zone electrophoresis.	a simple and rapid capillary zone electrophoresis method to quantitatively determine the phenolic acid contents in brassica vegetables is described. phenolic compounds were extracted from broccoli, broccolini, brussels sprouts, cabbage and cauliflower and the main hydroxycinnamic acids (sinapic, ferulic, p-coumaric and caffeic acids) were isolated by solid phase extraction with c18 cartridges. using an optimised method, the four analytes were separated in less than 7min in a 50μm×60cm capillary ...	2011	23140738
ethyl propiolate as a post-column derivatization reagent for thiols: development of a green liquid chromatographic method for the determination of glutathione in vegetables.	the present study reports the development, validation and application of a new green liquid chromatographic method for the determination of glutathione (gsh) in vegetable samples. in this work we introduce-for the first time-ethyl propiolate (ep) as an advantageous post-column derivatization reagent for thiolic compounds. gsh (t(r)=6.60 min) and n-acetylcysteine (nac, internal standard) (t(r)=11.80 min) were separated efficiently from matrix endogenous compounds by using a 100% aqueous mobile ph ...	2011	21414445
sulforaphane, a natural constituent of broccoli, prevents cell death and inflammation in nephropathy.	cisplatin (cis-diamminedichloroplatinum ii, cis) is a potent and widely used chemotherapeutic agent to treat various malignancies, but its therapeutic use is limited because of dose-dependent nephrotoxicity. cell death and inflammation play a key role in the development and progression of cis-induced nephropathy. sulforaphane (sfn), a natural constituent of cruciferous vegetables such as broccoli, brussels sprouts, etc., has been shown to exert various protective effects in models of tissue inju ...	2012	21684138
acceptance of health-promoting brassica vegetables: the influence of taste perception, information and attitudes.	to investigate the relative importance of specific health knowledge and taste on acceptance of brassica vegetables (broccoli, red and green cabbages, broccolini, cauliflower, brussels sprouts).	2012	22230576
the hazards of brussels sprouts consumption at christmas.		2012	23230944
potential of synthetic sex pheromone blend for mating disruption of the swede midge, contarinia nasturtii.	the potential for pheromone-based mating disruption of the brassica pest contarinia nasturtii was tested, both in small-scale plots with brussels sprouts and in commercial-scale fields with either broccoli or cauliflower. experiments in the small-scale plots used laboratory-reared insects released into a previously uninfested area, whereas large-scale experiments used a high natural population of c. nasturtii. effectiveness of mating disruption was evaluated by the reduction of male captures in ...	2012	22914960
nutritional quality of sous vide cooked carrots and brussels sprouts.	phytochemicals (carotenoids, phenolic compounds, and ascorbic acid) and antioxidant capacity (measured by teac, frap, and trap assays) were evaluated on carrots and brussels sprouts sous vide processed and then stored refrigerated for 1, 5, and 10 days and compared with the corresponding raw and oven-steamed products. data showed that sous vide cooked carrots had higher amounts of carotenoids, phenolic compounds, and ascorbic acid than steamed products, and only a slight decrease of phenolic com ...	2012	22568492
induction of phase 2 antioxidant enzymes by broccoli sulforaphane: perspectives in maintaining the antioxidant activity of vitamins a, c, and e.	consumption of fruits and vegetables is recognized as an important part of a healthy diet. increased consumption of cruciferous vegetables in particular has been associated with a decreased risk of several degenerative and chronic diseases, including cardiovascular disease and certain cancers. members of the cruciferous vegetable family, which includes broccoli, brussels sprouts, cauliflower, and cabbage, accumulate significant concentrations of glucosinolates, which are metabolized in vivo to b ...	2012	22303412
effect of polyphenols extracts from brassica vegetables on erythrocyte membranes (in vitro study).	the aim of this work was to estimate the in vitro effects of polyphenol extracts from brassica vegetables (brussels sprouts and red cabbage) on erythrocyte membranes with normal and high concentration of cholesterol. to determine the effect of phenolic compounds we prospectively studied cholesterol concentration, lipid peroxidation, membrane fluidity and atpase activity. polyphenol extracts from brassica vegetables resulted in statistically significant reductions in cholesterol concentrations in ...	2012	23044092
molecular cloning, characterization and analysis of the intracellular localization of a water-soluble chl-binding protein from brussels sprouts (brassica oleracea var. gemmifera).	a water-soluble chl-binding protein from brussels sprouts (brassica oleracea var. gemmifera), hereafter termed bowscp, is categorized into the class ii wscps (non-photoconvertible wscps). previous studies on bowscp have focused mainly on its biochemical characterization. in this study, we cloned the cdna encoding bowscp. sequence analysis revealed that the bowscp gene was composed of a single exon corresponding to 654 bp of an open reading frame encoding 218 amino acid residues, including 19 res ...	2012	22419824
insect egg deposition induces indirect defense and epicuticular wax changes in arabidopsis thaliana.	egg deposition by the large cabbage white butterfly pieris brassicae on brussels sprouts plants induces indirect defense by changing the leaf surface, which arrests the egg parasitoid trichogramma brassicae. previous studies revealed that this indirect defense response is elicited by benzyl cyanide (bc), which is present in the female accessory reproductive gland (arg) secretion and is released to the leaf during egg deposition. here, we aimed (1) to elucidate whether p. brassicae eggs induce pa ...	2012	22588570
consequences of constitutive and induced variation in the host's food plant quality for parasitoid larval development.	constitutive and induced changes in plant quality impact higher trophic levels, such as the development of parasitoids, in different ways. an efficient way to study how plant quality affects parasitoids is to examine how the parasitoid larva is integrated within the host during the growth process. in two experiments, we investigated the effects of varying nutritional quality of brassica oleracea on parasitoid larval development inside the host, the diamondback moth (plutella xylostella). first, ...	2012	22233934
bolbase: a comprehensive genomics database for brassica oleracea.	brassica oleracea is a morphologically diverse species in the family brassicaceae and contains a group of nutrition-rich vegetable crops, including common heading cabbage, cauliflower, broccoli, kohlrabi, kale, brussels sprouts. this diversity along with its phylogenetic membership in a group of three diploid and three tetraploid species, and the recent availability of genome sequences within brassica provide an unprecedented opportunity to study intra- and inter-species divergence and evolution ...	2013	24079801
molecular cloning and functional expression of a water-soluble chlorophyll-binding protein from japanese wild radish.	hydrophilic chlorophyll (chl)-binding proteins have been isolated from various brassicaceae plants and are categorized into class ii water-soluble chl-binding proteins (wscps). although the molecular properties of class ii wscps including brassica-type (e.g., cauliflower wscp, brussels sprouts wscp and bnd22, a drought- and salinity-stress-induced 22 kda protein of rapeseed), a lepidium-type, and an arabidopsis-type wscps have been well characterized, those of raphanus-type wscps are poorly unde ...	2013	23266282
diet and toenail arsenic concentrations in a new hampshire population with arsenic-containing water.	limited data exist on the contribution of dietary sources of arsenic to an individual's total exposure, particularly in populations with exposure via drinking water. here, the association between diet and toenail arsenic concentrations (a long-term biomarker of exposure) was evaluated for individuals with measured household tap water arsenic. foods known to be high in arsenic, including rice and seafood, were of particular interest.	2013	24237880
relationship between food preferences and prop taster status of college students.	how food tastes plays a key role in our food choices and eating behavior, with important implications for health and nutrition. the negative relationship of genetically predisposed sensitivity to 6-n-propylthiouracil (prop) and food preferences for bitter, creamy, and spicy foods, and alcohol is often reported in both scholarly and popular literature. our review of research indicates the empirical results are far from conclusive. we conducted a questionnaire-based study to examine enjoyment rati ...	2013	23648895
effects of brussels sprouts and their phytochemical components on oxidative stress-induced neuronal damages in pc12 cells and icr mice.	in this study, the protective effects of brussels sprouts extract and its major constituents against oxidative stress-induced damages were investigated in rat pheochromocytoma cells and institute of cancer research mice. the major constituents of brussels sprouts (3,4',5,7-tetrahydroxyflavone (kaempferol), indole-3-carbinol, and phenethyl isothiocyanate) were selectively tested. of these, the flavonoid compound, kaempferol exhibited the highest potency in radical scavenging activity (1,1-dipheny ...	2013	24175656
masking vegetable bitterness to improve palatability depends on vegetable type and taste phenotype.	consumption of dark green vegetables falls short of recommendations, in part, because of unpleasant bitterness. a laboratory-based study of 37 adults was used to determine bitter and hedonic responses to vegetables (asparagus, brussels sprouts, kale) with bitter masking agents (1.33 m sodium acetate, 10 and 32 mm sodium chloride, and 3.2 mm aspartame) and then characterized by taste phenotype and vegetable liking. in repeated-measures anova, aspartame was most effective at suppressing bitterness ...	2013	23682306
characterisation of taste-active extracts from raw brassica oleracea vegetables.	chemical and sensory characterisation of whole and fractionated myrosinase-free extracts from selected australian-grown, raw brassica vegetables (broccoli, cauliflower, brussels sprouts and red cabbage) was carried out to determine the contribution of key phytochemicals (i.e. glucosinolates, free sugars, phenolics) to the taste profiles of these vegetables. glucosinolate (gs) and phenolic profiles were determined by liquid chromatography coupled with photodiode array detection and mass spectrome ...	2013	23370542
[vitamin concentration in different varieties and grades of cabbage].	examinations covered 8 varieties (28 grades) of cabbage for the content therein of c, b6 vitamins, beta-carotene, thiamine, riboflavin, niacin and folacin. all the cabbage specimens were analyzed right after picking. in 2 grades of the cabbage vitamins were determined after picking and repeatedly after storage (9 months in a vegetable storehous). it was found that there existed a significant difference in the quantitative content of vitamins between individual varieties of cabbage. the proportio ...	2013	982952
the c-terminal extension peptide of non-photoconvertible water-soluble chlorophyll-binding proteins (class ii wscps) affects their solubility and stability: comparative analyses of the biochemical and chlorophyll-binding properties of recombinant brassica, raphanus and lepidium wscps with or without their c-terminal extension peptides.	numerous members of the brassicaceae possess non-photoconvertible water-soluble chlorophyll (chl)-binding proteins (class ii wscps), which function as chl scavengers during cell disruption caused by wounding, pest/pathogen attacks, and/or environmental stress. class ii wscps have two extension peptides, one at the n-terminus and one at the c-terminus. the n-terminal peptide acts as a signal peptide, targeting the protein to the endoplasmic reticulum body, a unique defensive organelle found only ...	2014	24389799
associative conditioning can increase liking for and consumption of brussels sprouts in children aged 3 to 5 years.	pairing foods with liked flavors repeatedly can increase preferences for those foods. we compared the effectiveness of associative conditioning (pairing vegetables with sweetened and unsweetened cream cheese) and exposure (presenting vegetables alone) in increasing liking and consumption of bitter and nonbitter vegetables. twenty-nine children (aged 3 to 5 years) participated in the study. one group of children received brussels sprouts (bitter) with sweetened cream cheese and cauliflower (nonbi ...	2014	24439970
impact of selenium supply on se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified brassica sprouts.	brassica sprouts are widely marketed as functional foods. here we examined the effects of se treatment on the accumulation of anticancer compound se-methylselenocysteine (semscys) and glucosinolates in brassica sprouts. cultivars from the six most extensively consumed brassica vegetables (broccoli, cauliflower, green cabbage, chinese cabbage, kale, and brussels sprouts) were used. we found that se-biofortified brassica sprouts all were able to synthesize significant amounts of semscys. analysis ...	2014	25038715
sulforaphane protects h9c2 cardiomyocytes from angiotensin ii-induced hypertrophy.	cardiac hypertrophy is an adaptive process of the heart in response to various stimuli, but sustained cardiac hypertrophy will finally lead to heart failure. sulforaphane-extracted from cruciferous vegetables of the genus brassica such as broccoli, brussels sprouts, and cabbage-has been evaluated for its anticarcinogenic and antioxidant effects.	2014	23784363
isothiocyanate synthetic analogs: biological activities, structure-activity relationships and synthetic strategies.	sulforaphane is a natural product that is constantly under biological investigation for its unique biological properties. this naturally occurring isothiocyanate (itc) and its analogs are the main components of cruciferous vegetables, such as cauliflower, watercress, broccoli, cabbage, brussels sprouts, widely used as chemopreventive agents. due to their interesting biological profiles, natural itcs have been exploited as starting point to develop new synthetic analogs. the present mini-review b ...	2014	25373847
low levels of 3,3'-diindolylmethane activate estrogen receptor α and induce proliferation of breast cancer cells in the absence of estradiol.	3,3'-diindolylmethane (dim) is an acid-catalyzed dimer of idole-3-carbinol (i3c), a phytochemical found in cruciferous vegetables that include broccoli, brussels sprouts and cabbage. dim is an aryl hydrocarbon receptor (ahr) ligand and a potential anticancer agent, namely for the treatment of breast cancer. it is also advertised as a compound that regulates sex hormone homeostasis.	2014	25048790
the antiproliferative response of indole-3-carbinol in human melanoma cells is triggered by an interaction with nedd4-1 and disruption of wild-type pten degradation.	human melanoma cells displaying distinct pten genotypes were used to assess the cellular role of this important tumor-suppressor protein in the antiproliferative response induced by the chemopreventative agent indole-3-carbinol (i3c), a natural indolecarbinol compound derived from the breakdown of glucobrassicin produced in cruciferous vegetables such as broccoli and brussels sprouts. i3c induced a g1-phase cell-cycle arrest and apoptosis by stabilization of pten in human melanoma cells that exp ...	2014	25009292
urinary 3,3'-diindolylmethane: a biomarker of glucobrassicin exposure and indole-3-carbinol uptake in humans.	brassica vegetable consumption may confer a protective effect against cancer, possibly attributable to their glucosinolates. glucobrassicin is a predominant glucosinolate and is the precursor of indole-3-carbinol (i3c), a compound with anticancer effects. however, objective assessments of i3c uptake from brassica vegetables have not been successful.	2014	24357105
attenuation of carcinogenesis and the mechanism underlying by the influence of indole-3-carbinol and its metabolite 3,3'-diindolylmethane: a therapeutic marvel.	rising evidence provides credible support towards the potential role of bioactive products derived from cruciferous vegetables such as broccoli, cauliflower, kale, cabbage, brussels sprouts, turnips, kohlrabi, bok choy, and radishes. many epidemiological studies point out that brassica vegetable protects humans against cancer since they are rich sources of glucosinolates in addition to possessing a high content of flavonoids, vitamins, and mineral nutrients. indole-3-carbinol (i3c) belongs to th ...	2014	24982671
cutting brussels sprouts: collaboration involving persons with dementia.	how people with dementia collaborate with other people is an area in need of more research and conceptualizations. collaboration introduces a number of new possibilities and demands concerning cognitive and linguistic abilities and it is suggested that a theoretical framework that emphasize that cognitive resources are not exclusively individual, but are part of cognitive and communicative context. in this article focus is on joint activities and their collaborative organization is analyzed usin ...	2014	24655679
brpspl9 (brassica rapa ssp. pekinensis spl9) controls the earliness of heading time in chinese cabbage.	the leafy heads of cabbage (brassica oleracea), chinese cabbage (brassica rapa ssp. pekinensis), brussels sprouts (b. oleracea ssp. gemmifera) and lettuce (lactuca sativa) comprise extremely incurved leaves that are edible vegetable products. the heading time is important for high quality and yield of these crops. here, we report that brpspl9-2 (b. rapa ssp. pekinensis squamosa promoter binding-like 9-2), a target gene of microrna brp-mir156, controls the heading time of chinese cabbage. quantit ...	2014	24237584
jack bean urease inhibition by crude juices of allium and brassica plants. determination of thiosulfinates.	the aim of this study was the elucidation of the inhibitory influence of allium (garlic, onion, leek) and brassica (cabbage, brussels sprouts) plants juices, on jack bean urease activity. concentrations of thiosulfinates, the compounds responsible for the inhibition, were determined in studied materials. the kinetics and mechanism of the inhibitions were investigated. biphasic, time-dependent courses of the inhibition reactions were observed for all tested allium and brussels sprouts from brassi ...	2014	24128461
determination of sulphur in various vegetables by solid sampling high-resolution electrothermal molecular absorption spectrometry.	sulphur was determined in various vegetables via molecular absorption of carbon monosulphide (cs) at 258.056 nm using a solid sampling high resolution continuum source electrothermal atomic absorption spectrometer (ss hr-cs etaas). samples were dried, ground and directly introduced into the ruthenium coated graphite furnace as 0.05 to 0.50mg. all determinations were performed using palladium+citric acid modifier and applying a pyrolysis temperature of 1000 °c and a volatilisation temperature of ...	2015	25442545
rapeseed phosphatidylcholine hydrolysis to phosphatidic acid using plant extracts with phopspholipase d.	phosphatidic acid (pa) has a crucial role in cell membrane structure and function. for that reason it has a possible application in the treatment of some health disorders in humans, can be used as a natural and non toxic emulsifier and the component of drug carriers in pharmaceuticals and cosmetics as well as a component for synthesis of some new phospholipids. pa is short-lived in the cell and is difficult to extract directly from the biological material. pa may be easily prepared by hydrolysis ...	2015	26642684
liquid chromatography-mass spectrometry coupled with multivariate analysis for the characterization and discrimination of extractable and nonextractable polyphenols and glucosinolates from red cabbage and brussels sprout waste streams.	nonextractable polyphenol (nep) fractions are usually ignored because conventional extraction methods do not release them from the plant matrix. in this study, we optimized the conditions for sonicated alkaline hydrolysis to the residues left after conventional polyphenol extraction of brussels sprouts top (80°c, 4m naoh, 30min) and stalks (60°c, 4m naoh, 30min), and red cabbage waste streams (80°c, 4m naoh, 45min) to release and characterize the nep fraction. the nep fractions of brussels sprou ...	2015	26008597
effect of cruciferous vegetables on lung cancer in patients stratified by genetic status: a mendelian randomisation approach.	whether consumption of cruciferous vegetables protects against lung cancer is unclear, largely because of potential confounding factors. we therefore studied the role of cruciferous vegetables in lung cancer after stratifying by gstm1 and gstt1 status, two genes implicated in the elimination of isothiocyanates, the likely chemopreventative compound. in 2141 cases and 2168 controls, weekly consumption of cruciferous vegetables protected against lung cancer in those who were gstm1 null (odds ratio ...	2015	16257343
role of large cabbage white butterfly male-derived compounds in elicitation of direct and indirect egg-killing defenses in the black mustard.	to successfully exert defenses against herbivores and pathogens plants need to recognize reliable cues produced by their attackers. up to now, few elicitors associated with herbivorous insects have been identified. we have previously shown that accessory reproductive gland secretions associated with eggs of cabbage white butterflies (pieris spp.) induce chemical changes in brussels sprouts plants recruiting egg-killing parasitoids. only secretions of mated female butterflies contain minute amoun ...	2015	26483811
assessment of heterogeneity in types of vegetables served by main household food preparers and food decision influencers.	while vegetables are often studied as one food group, global measures may mask variation in the types and forms of vegetables preferred by different individuals. to explore preferences for and perceptions of vegetables, we assessed main food preparers based on their preparation of eight specific vegetables and mushrooms.	2015	25850676
the epigenetic impact of cruciferous vegetables on cancer prevention.	the answer to chemoprevention has perhaps been available to the general public since the dawn of time. the epigenetic diet is of extreme interest, for research suggests that cruciferous vegetables are not only an important source of nutrients, but perhaps a key to eliminating cancer as life threatening disease. cruciferous vegetables such as kale, cabbage, brussels sprouts, and broccoli sprouts contain chemical components, such as sulforaphane (sfn) and indole-3-carbinol (i3c), which have been r ...	2015	25774338
anteiso-fatty acids in brussels sprouts (brassica oleracea var. gemmifera l.): quantities, enantioselectivities, and stable carbon isotope ratios.	anteiso-fatty acids (afas) are a class of branched-chain fatty acids that are characterized by one methyl branch on the antepenultimate carbon of the straight acyl chain. afas are mainly produced by bacteria, and sources in vegetables are scarce. this study reports the concentrations of odd-numbered afas (a15:0-a21:0) in brussels sprout buds. selective enrichment followed by enantioselective gas chromatography with mass spectrometry in the selected ion monitoring mode revealed that both a15:0 an ...	2015	26390192
cognition enhancing activity of sulforaphane against scopolamine induced cognitive impairment in zebra fish (danio rerio).	several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (broccoli and brussels sprouts) can protect against chronic diseases. sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in several experimental paradigms. this study was undertaken to examine the effect of sulforaphane on cognitive impairment in zebra fish model using a novel method of fear conditioning. ...	2016	27255600
sinigrin and its therapeutic benefits.	sinigrin (allyl-glucosinolate or 2-propenyl-glucosinolate) is a natural aliphatic glucosinolate present in plants of the brassicaceae family, such as broccoli and brussels sprouts, and the seeds of brassica nigra (mustard seeds) which contain high amounts of sinigrin. since ancient times, mustard has been used by mankind for its culinary, as well as medicinal, properties. it has been systematically described and evaluated in the classical ayurvedic texts. studies conducted on the pharmacological ...	2016	27043505
preservation of brussels sprouts by pullulan coating containing oregano essential oil.	in this study, the effectiveness of pullulan (a fungal polysaccharide) film containing oregano essential oil (oeo) at 1.0 to 10.0% was evaluated against bacteria, yeasts, and molds. the quality of the sprouts, as determined by weight loss, color, and appearance, was monitored during storage at 2 and 16°c. an organoleptic evaluation of odor preference and odor acceptability of oeo on the brussels sprouts was also conducted. the antimicrobial activity of pullulan films with oeo increased significa ...	2016	26939662
brussels sprout decapitation yields larger sprouts of superior quality.	a common technological practice in brussels sprouts (brassica oleracea l. var. gemmifera) production is the removal of apical plant section a few weeks before harvest in order to promote the development of auxiliary buds (sprouts) and ensure higher yields. it is well-known that this measure positively influences the size of the sprouts, but until now no study has focused on the effect of decapitation on the content of primary and secondary metabolites in brussels sprouts. plants were decapitated ...	2016	27626626
myrosinase compatible simultaneous determination of glucosinolates and allyl isothiocyanate by capillary electrophoresis micellar electrokinetic chromatography (ce-mekc).	the functional food cruciferous vegetables contain glucosinolates which are decomposed by the myrosinase enzyme upon tissue damage. the isothiocyanates are the most frequent decomposition products. because of their various bioactivities, these compounds and the myrosinase is of high interest to many scientific fields.	2016	27313156
concentrations of thiocyanate and goitrin in human plasma, their precursor concentrations in brassica vegetables, and associated potential risk for hypothyroidism.	brassica vegetables are common components of the diet and have beneficial as well as potentially adverse health effects. following enzymatic breakdown, some glucosinolates in brassica vegetables produce sulforaphane, phenethyl, and indolylic isothiocyanates that possess anticarcinogenic activity. in contrast, progoitrin and indolylic glucosinolates degrade to goitrin and thiocyanate, respectively, and may decrease thyroid hormone production. radioiodine uptake to the thyroid is inhibited by 194 ...	2016	26946249
effect of hydrothermal processing on phenolic acids and flavonols contents in selected brassica vegetables.	commonly occurring diseases can have the origin in oxidative processes ongoing in the human body. vegetables of brassicaceae family are the essential sources of natural antioxidants, especially phenolic compounds, in the human diet. the research was aimed to estimate the content of phenolic compounds in selected vegetables and their quantity changes during hydrothermal processes.	2017	22230974
enantioselectivity of anteiso-fatty acids in hitherto uninspected sample matrices.	anteiso-fatty acids (afas) are chiral molecules due to a methyl substituent on the antepenultimate carbon of the otherwise straight acyl chain. 12-methyltetradecanoic acid (a15:0) and 14-methylhexadecanoic acid (a17:0) are the predominant afas in nature but their individual contributions e.g. to food lipids are usually low. enantioselective data has been collected in fish, bovine milk/cheese, and brussels sprouts. in this study, we determined the enantioselectivity of a15:0 and a17:0 in shea but ...	2017	28753519
sinigrin attenuates the progression of atherosclerosis in apoe-/- mice fed a high-cholesterol diet potentially by inhibiting vcam-1 expression.	atherosclerosis is a complex inflammatory disease associated with elevated levels of atherogenic molecules for leukocyte recruitment. sinigrin (2-propenylglucosinolate) is found mainly in broccoli, brussels sprouts, and black mustard seeds. recently, sinigrin has received attention for its role in disease prevention and health promotion. in this study, we examined the effect of sinigrin on development of atherosclerosis in apoe-/- mice and the expression of adhesion molecules in vascular smooth ...	2017	28483571
coumestrol inhibits proliferation and migration of prostate cancer cells by regulating akt, erk1/2, and jnk mapk cell signaling cascades.	coumestrol is the one of the major phytoestrogens which is abundant in soybeans, legumes, brussel sprouts, and spinach. the beneficial effects of coumestrol are well known in various biological processes including; neuroprotective effects on the nervous system, function of the female reproductive system, anti-bacterial properties, and anti-cancer effects. although the anti-tumor activity of coumestrol has been demonstrated for ovarian, breast, lung, and cervical cancers, little is known of its e ...	2017	27431052
genetic diversity and population structure of brassica oleracea germplasm in ireland using ssr markers.	the most economically important brassica oleracea species is endangered in ireland, with no prior reported genetic characterization studies. this study assesses the genetic diversity, population structure and relationships of b. oleracea germplasm in ireland using microsatellite (ssrs) markers. a total of 118 individuals from 25 accessions of irish b. oleracea were genotyped. the ssr loci used revealed a total of 47 alleles. the observed heterozygosity (0.699) was higher than the expected one (0 ...	2017	26995396
sinigrin inhibits production of inflammatory mediators by suppressing nf-κb/mapk pathways or nlrp3 inflammasome activation in macrophages.	sinigrin (2-propenyl glucosinolate) is found mainly in broccoli, brussels sprouts, and black mustard seeds. recently, sinigrin has received attention for its role in disease prevention and health. this study investigated the effect of sinigrin on macrophage function, including the activity of nod-like receptor protein 3 (nlrp3) inflammasome. in a concentration-dependent manner, sinigrin inhibited lipopolysaccharide (lps)-induced nitric oxide (no) production and the expression of cox-2 and prosta ...	2017	28219839
application and optimisation of air-steam cooking on selected vegetables: impact on physical and antioxidant properties.	several studies investigated the impact of different cooking techniques on the quality of vegetables. however, the use of the combined air-steam cooking is still scarcely debated, despite the advantages informally referred by professional catering workers. in this study, its optimisation was studied on brussels sprouts and pumpkin cubes to obtain the best physical (texture, colour) and antioxidant (frap, total phenols) response, in comparison to a conventional steaming treatment.	2017	28984926
is smog innocuous? air pollution and cardiovascular disease.	air pollution is a significant environmental and health hazard. earlier studies had examined the adverse health effects associated with short- and long-term exposure to particulate matter on respiratory disease. however, later studies demonstrated that was actually cardiovascular disease that accounted for majority of mortality. furthermore, it was not gaseous pollutants like oxides of nitrate, sulfur, carbon mono-oxide or ozone but the particulate matter or pm, of fine or coarse size (pm2.5 and ...	2017	28822504
in vitro bile acid binding capacities of red leaf lettuce and cruciferous vegetables.	in the present study, we tested the bile acid binding capacity of red leaf lettuce, red cabbage, red kale, green kale, and brussels sprouts through in vitro digestion process by simulating mouth, gastric, and intestinal digestion using six bile acids at physiological ph. green and red kale exhibited significantly higher (86.5 ± 2.9 and 89.7 ± 0.9%, respectively) bile acid binding capacity compared to the other samples. further, three different compositions of bile acids were tested to understand ...	2017	28812344
effect of equilibrated ph and indigenous spoilage microorganisms on the inhibition of proteolytic clostridium botulinum toxin production in experimental meals under temperature abuse.	clostridium botulinum is a foreseeable biological hazard in prepared refrigerated meals that needs to be addressed in food safety plans. the objective of this study was to evaluate the effect of product composition and storage temperature on the inhibition of botulinum toxin formation in nine experimental meals (meat, vegetable, or carbohydrate based). treatments were inoculated with proteolytic c. botulinum, vacuum packaged, cooked at 90°c for 10 min, and assayed for botulinum toxin in samples ...	2017	28686492
influence of cooking methods on bioactive compound content and antioxidant activity of brussels sprouts.	the effects of different cooking methods on total bioactive compound content were determined, and in vitro antioxidant activity in 80% ethanolic extracts of brussels sprouts was evaluated by spectrophotometric methods. compared to uncooked, steamed, and microwaved brussels sprouts extracted with 80% ethanol contained higher amounts of total polyphenols. uncooked brussels sprouts contained the highest amounts of total flavonoids. microwaved brussels sprouts contained the highest amounts of total ...	2017	29333389
high pressure effects on myrosinase activity and glucosinolate preservation in seedlings of brussels sprouts.	combinations of pressure, temperature and time (100-600 mpa, 30-60 °c, 3-10 min) influence enzyme activity of the myrosinase-glucosinolate system. seedlings of brussels sprouts were used as a model, which constitutes a well-defined and homogenous sample matrix with simple cell structures. a response surface methodology approach was used to determine the combined effect of pressure level, temperature and time on glucosinolate concentration and myrosinase activity in brussels sprouts seedlings. th ...	2018	29287344
identification and analysis of a mercapturic acid conjugate of indole-3-methyl isothiocyanate in the urine of humans who consumed cruciferous vegetables.	glucobrassicin, a quantitatively significant constituent of brassica vegetables, gives rise to indole-3-carbinol (i3c) and its dimer di-indolylmethane (dim) when the vegetables are chewed. i3c and dim have been extensively studied with respect to their anti-carcinogenic properties. however, the presumed intermediate isothiocyanate in their formation, indole-3-methyl isothiocyanate (imitc), has to our knowledge never been observed, despite the fact that isothiocyanates derived from cruciferous ve ...	2018	29223047