| antifungal activity of gallic acid purified from terminalia nigrovenulosa bark against fusarium solani. | the antifungal activities of methanolic extracts from terminalia nigrovenulosa bark (tnb) was investigated for effects on the initial growth of mycelia against fusarium solani. the ethyl acetate fraction separated from tnb demonstrated the highest antifungal activity against f. solani. the antifungal compound was isolated from tnb using silica gel column and sephadex lh-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. structural identification ... | 2013 | 23333407 |
| the use of response surface methodology as a statistical tool for media optimization in lipase production from the dairy effluent isolate fusarium solani. | the optimization of extracellular lipase production by fusarium isolani strain skwf7 isolated from dairy wastewater was carried out in this study. initially, the physicochemical factors significantly influencing enzyme production were studied by varying one-factor-at-a-time (ofat). a mesophilic temperature of 40°c, alkaline ph of 8, and incubation period of 72 hours were found to be the optimal conditions for lipase production. among the media components, the disaccharide sucrose acted as the be ... | 2013 | 25969775 |
| fungal mitochondrial dnases: effectors with the potential to activate plant defenses in nonhost resistance. | previous reports on the model nonhost resistance interaction between fusarium solani f. sp. phaseoli and pea endocarp tissue have described the disease resistance-signaling role of a fungal dnase1-like protein. the response resulted in no further growth beyond spore germination. this f. solani f. sp. phaseoli dnase gene, constructed with a pathogenesis-related (pr) gene promoter, when transferred to tobacco, generated resistance against pseudomonas syringe pv. tabaci. the current analytical/theo ... | 2013 | 23228145 |
| a novel chitin-binding protein from moringa oleifera seed with potential for plant disease control. | a thermostable chitin-binding protein (14.3 kda) with antifungal activity was isolated from moringa oleifera seeds by affinity chromatography on chitin followed by ion exchange chromatography. nh(2-) cpaiqrccqqlrniqppcrccq (mo-cbp3) is a glycoprotein with 2.5% sugar, pi 10.8, without hemagglutination, chitinase or beta-glucanase activities. mo-cbp3 possesses in vitro antifungal activity against the phytopathogenicfungi fusarium solani, f. oxysporum, colletotrichum musae and c. gloesporioides. co ... | 2012 | 23193603 |
| determination of organophosphorus and carbamate insecticides in fresh fruits and vegetables by high-performance thin-layer chromatography-multienzyme inhibition assay. | hptlc-enzyme inhibition assay was applied to different fruit and vegetable samples after individual spiking with organophosphate and carbamate pesticides at their maximum residue limits documented by the european commission. samples were extracted according to the quechers (quick, easy, cheap, effective, rugged, and safe) method, including cleanup by primary secondary amine sorbent. additional cleanup was performed on the hptlc plate by a prechromatographic step to separate most coextracted matr ... | 2012 | 23175968 |
| purification and biochemical characterization of a novel alkaline (phospho)lipase from a newly isolated fusarium solani strain. | an extracellular lipase from fusarium solani strain (f. solani lipase (fsl)) was purified to homogeneity by ammonium sulphate precipitation, gel filtration and anion exchange chromatography. the purified enzyme has a molecular mass of 30 kda as estimated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. the 12 nh(2)-terminal amino acid residues showed a high degree of homology with a putative lipase from the fungus necteria heamatoccocae. it is a serine enzyme, like all known lipase ... | 2012 | 23151966 |
| evaluation of in vitro antioxidant potency of exopolysaccharide from endophytic fusarium solani sd5. | a potent endophytic fungus, fusarium solani sd5 was used for exopolysaccharide (eps) production. the isolated eps were purified and major eps fraction (ps-i); rhamno galactan was used to evaluate anti oxidant activities in vitro. eps (ps-i) showed significant free radical scavenging effect on dpph (1,1-diphenyl-2-picrylhydrazyl) and scavenging potency is indicated by ic(50) value 578.541 ± 33.256 μg/ml. eps (ps-i) significantly induced antioxidant parameters of peritoneal macrophage cells at a c ... | 2013 | 23148947 |
| kinetic properties of a novel fusarium solani (phospho)lipase: a monolayer study. | using the monomolecular film technique, we studied interfacial properties of fusarium solani lipase (fsl). this lipolytic enzyme was found to be unique among the fungal lipases possessing not only a lipase activity but also a high phospholipase one.the fsl was able to hydrolyze dicaprin films at various surface pressures. the surface pressure dependency, the stereospecificity, and the regioselectivity of fsl were performed using optically pure stereoisomers of diglyceride (1,2-sn- dicaprin and 2 ... | 2013 | 23124905 |
| an automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform. | high-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. here, the nature of the foreign protein makes it impossible to predict the, e.g. best expression construct, secretion signal peptide, inductor concentration, induction time, temperature and sub ... | 2012 | 23113930 |
| synthesis and kinetic evaluation of cyclophostin and cyclipostins phosphonate analogs as selective and potent inhibitors of microbial lipases. | a new series of customizable diastereomeric cis- and trans-monocyclic enol-phosphonate analogs to cyclophostin and cyclipostins were synthesized. their potencies and mechanisms of inhibition toward six representative lipolytic enzymes belonging to distinct lipase families were examined. with mammalian gastric and pancreatic lipases no inhibition occurred with any of the compounds tested. conversely, fusarium solani cutinase and lipases from mycobacterium tuberculosis (rv0183 and lipy) were all f ... | 2012 | 23095026 |
| impact of contact lens materials on multipurpose contact lens solution disinfection activity against fusarium solani. | to investigate the effects of eight different soft contact lenses on disinfection efficacy of a multipurpose solution (mps) containing polyhexamethylene biguanide (phmb) against fusarium solani. | 2012 | 23085618 |
| successful treatment of a severe case of fusariomycosis in a beluga whale (delphinapterus leucas leucas). | an adult male beluga whale (delphinapterus leucas leucas) was presented with a 4-cm-diameter, raised, firm nodule on the medial aspect of the left pectoral fin. a fissure developed within the center of the nodule, which formed an ulcerated cyst-like lesion. the lesion rapidly progressed in size, and, with peeling of material present within the cyst, the lesion flattened to a 36 x 25-cm cutaneous ulcer that extended into the axilla. histopathologic features were consistent with lymphocytic and su ... | 2012 | 23082525 |
| disseminated amphotericin-resistant fusariosis in acute leukemia patients: report of two cases. | disseminated fusariosis has emerged as a significant, usually fatal infection in immunocompromised hosts despite antifungal treatment. we describe here two patients with acute leukemia who developed disseminated amphotericin-resistant fusariosis, and review of six studies of cases series in the literature. two fusarium solani strains were isolated from blood and skin cultures of one patient, and one strain from the blood culture of the second patient. both patients died despite antifungal treatm ... | 2013 | 23076561 |
| dermatitis and systemic mycosis in lined seahorses hippocampus erectus associated with a marine-adapted fusarium solani species complex pathogen. | during a 4 mo epizootic, 100% of 152 lined seahorses hippocampus erectus in 3 separate groups died while in quarantine following shipment to a public aquarium. twelve animals with skin depigmentation and ulceration were received by the aquatic pathology service, college of veterinary medicine, university of georgia, athens, georgia, usa, for diagnostic evaluation. microscopically, lesions in 11 seahorses included multifocal epithelial necrosis and ulceration associated with 2 to 7 µm diameter, b ... | 2012 | 23047188 |
| high-throughput sequencing of black pepper root transcriptome. | black pepper (piper nigrum l.) is one of the most popular spices in the world. it is used in cooking and the preservation of food and even has medicinal properties. losses in production from disease are a major limitation in the culture of this crop. the major diseases are root rot and foot rot, which are results of root infection by fusarium solani and phytophtora capsici, respectively. understanding the molecular interaction between the pathogens and the host's root region is important for obt ... | 2012 | 22984782 |
| docking and molecular dynamics studies of peptide inhibitors of ornithine decarboxylase: a rate-limiting enzyme for the metabolism of fusarium solani. | fusarium solani causes a wide variety of diseases in plants. polyamine biosynthesis is responsible for the growth and pathogenicity of the fungus. the initial step of this pathway involves the decarboxylation of ornithine to putrescine, and is catalyzed by the enzyme ornithine decarboxylase (odc). inhibiting this process may be a promising approach for the management of fungal disease in various crops. therefore, there is a need to develop inhibitors of odc that have higher binding capacity than ... | 2013 | 22970930 |
| structural elucidation and bioactivity of a novel exopolysaccharide from endophytic fusarium solani sd5. | a bioactive exopolysaccharide [eps (ps-i)], having mw∼1.87×10(5) da was produced by submerged culture of an endophytic fungus fusarium solani sd5. structural elucidation of the eps (ps-i) was carried out by a series of experiments. result indicates the presence of terminal α-l-rhamnopyranosyl, (1→2)-α-l-rhamnopyranosyl, (1→4)-β-d-galactopyranosyl, (1→4,6)-β-d-galactopyranosyl moieties in a molar ratio of nearly 1:1:3:1. tem image showed fibril structure of the eps with a diameter of approximatel ... | 2012 | 24751093 |
| [genetic diversity of 21 fusarium strains in section martiella based on issr analysis]. | in order to understand the genetic difference and phylogenic relationship within and among the fusarium species in section martiella, the genetic diversity of 21 fusarium strains in the section was examined by inter-simple sequence repeats (issr). fifteen selected issr primers were adopted to do amplification, and a total of 239 bands were amplified, among which, 230 (96.2%) were polymorphic, with an average of 15.3 polymorphic bands per primer. the genetic similarity ranged from 0.494 to 0.933, ... | 2012 | 22919846 |
| fusarium solani: a causative agent of skin and nail infections. | fusarium spp are non-dermatophytic hyaline moulds found as saprophytes and plant pathogens. human infections are probably a result of various precipitating predisposing factors of impaired immune status. immunocompetent individuals of late are also vulnerable to various unassuming saprophytic and plant pathogens. to stress the need to identify correctly and institute appropriate antifungal therapy in newly emerging human fungal infectious agents. repeated mycological sampling of the skin and nai ... | 2012 | 22837572 |
| comparison of two dna sequence-based typing schemes for the fusarium solani species complex and proposal of a new consensus method. | multilocus sequence typing (mlst) is a widely used approach for differentiating microbial isolates presenting many advantages such as easy access through online databases and straightforward interpretation. for the fusarium solani species complex (fssc), three gene regions have been widely used to investigate phylogenetic relationships at the interspecific level (its-nulsu, ef1a, rpb2) and a nomenclature system has been proposed for the different known haplotypes. more recently, a mlst scheme wa ... | 2012 | 22820199 |
| surgical removal of an abscess associated with fusarium solani from a kemp's ridley sea turtle (lepidochelys kempii). | a cold-stunned kemp's ridley sea turtle, lepidochelys kempii, developed an abscess associated with fusarium solani, vibrio alginolyticus, and a shewenalla species after receiving a bite wound to the front flipper during rehabilitation. the lesion failed to respond to medical therapy and was treated successfully with surgery. histopathology of the excised tissue demonstrated septic heterophilic inflammation with necrosis and granulation tissue, fungal elements, and bacteria, despite appropriate a ... | 2012 | 22779250 |
| early expression of surfactant proteins d in fusarium solani infected rat cornea. | to investigate the early expression of surfactant proteins d(sp-d) in fusarium solani infected rat cornea. | 2012 | 22773976 |
| antifungal efficacy of natamycin in experimental fusarium solani keratitis. | to evaluate the efficacy of topical administration natamycin, which is produced by china, in an experimental rabbit model of fusarium solani keratitis, to provide experimental basis for the application of clinical safety. | 2012 | 22762038 |
| intra-articular injection of voriconazole for fusarium solani arthritis after bone marrow transplantation. | | 2012 | 22705652 |
| evaluation of the antifungal activity of zataria multiflora, geranium herbarium, and eucalyptus camaldolensis essential oils on saprolegnia parasitica-infected rainbow trout (oncorhynchus mykiss) eggs. | the purpose of the present study was to evaluate and assess the capability of zataria multiflora, geranium herbarium, and eucalyptus camaldolensis essential oils in treating saprolegnia parasitica-infected rainbow (oncorhynchus mykiss) trout eggs. a total of 150 infected eggs were collected and plated on glucose-pepton agar at 24°c for 2 weeks. the antifungal assay of essential oils against s. parasitica was determined by a macrodilution broth technique. the eggs were treated with essential oils ... | 2012 | 22690761 |
| compost as a source of microbial isolates for the bioremediation of heavy metals: in vitro selection. | heavy metal pollution has become a major environmental concern nowadays and the bioremediation of polluted habitats is an increasingly popular strategy due to both its efficiency and safety. a screening and selection protocol based on different composting processes was designed in order to isolate heavy metal-resistant microorganisms. a collection of 51 microorganisms was obtained and most of them showed the capability to tolerate heavy metals in multi-polluted aqueous systems (cd(ii), cr(vi), n ... | 2012 | 22664539 |
| successful medical management of recalcitrant fusarium solani keratitis: molecular identification and susceptibility patterns. | fungal keratitis is a rare but sight-threatening infection of the cornea that may be caused by several fungal pathogens. a delay in diagnosis and inadequate treatment may even lead to loss of the affected eye. fungal keratitis is often misdiagnosed as bacterial keratitis because isolation and identification of the fungal pathogen is difficult and requires experience, and fungal growth in culture requires time. in this report, a 14-year-old boy with recalcitrant fusarium solani keratitis, unrespo ... | 2012 | 22528742 |
| evidence for a novel biological role for the multifunctional β-1,3-glucan binding protein in shrimp. | β-1,3-glucan binding proteins (βgbps) are soluble pattern recognition proteins/receptors that bind to β-1,3-glucans from fungi cell walls. in crustaceans, βgbps are abundant plasmatic proteins produced by the hepatopancreas, and have been proved to play multiple biological functions. here, we purified and characterized novel members of the βgbp family from the hemolymph of two brazilian shrimps, farfantepenaeus paulensis (fpβgbp) and litopenaeus schmitti (lsβgbp). as observed for other crustacea ... | 2012 | 22525007 |
| ultraviolet induction of antifungal activity in plants. | ultraviolet-c irradiation as a method to induce the production of plant compounds with antifungal properties was investigated in the leaves of 18 plant species. a susceptibility assay to determine the antifungal susceptibility of filamentous fungi was developed based on an agar dilution series in microtiter plates. uv irradiation strongly induced antifungal properties in five species against a clinical fusarium solani strain that was responsible for an onychomycosis case that was resistant to cl ... | 2012 | 22509892 |
| fungi associated with scolytogenes birosimensis (coleoptera: curculionidae) infesting pittosporum tobira. | the bark beetle scolytogenes birosimensis niijima is suspected to be involved in the decline of pittosporum tobira (thunb. ex murray) aiton in the coastal areas of japan. we isolated fungi from adult s. birosimensis in nine different localities in japan to assess their potential association and predict their contribution to the success of the beetle. results from morphological identification of associated fungi showed that the beetle was associated with fusarium solani and candida spp. furthermo ... | 2012 | 22506997 |
| structural analysis of a new cytotoxic demethylated analogue of neo-n-methylsansalvamide with a different peptide sequence produced by fusarium solani isolated from potato. | a novel cytotoxic cyclic pentadepsipeptide, neosansalvamide, was produced by fusarium solani kccm90040 isolated from fusarium -contaminated potato in korea. the molecular formula of neosansalvamide was analyzed as c₃₂h₅₀n₄o₆ by electrospray ionization tandem mass spectrometry and combined structural analysis. the one- and two-dimensional nuclear magnetic resonance and absolute configuration of amino acid spectral data allowed for the resolution of cyclic five subunits linked in the following ord ... | 2012 | 22502643 |
| proteomic analysis of fusarium solani isolated from the asian longhorned beetle, anoplophora glabripennis. | wood is a highly intractable food source, yet many insects successfully colonize and thrive in this challenging niche. overcoming the lignin barrier of wood is a key challenge in nutrient acquisition, but full depolymerization of intact lignin polymers has only been conclusively demonstrated in fungi and is not known to occur by enzymes produced by insects or bacteria. previous research validated that lignocellulose and hemicellulose degradation occur within the gut of the wood boring insect, an ... | 2012 | 22496740 |
| production of lignocellulose-degrading enzymes employing fusarium solani f-552. | in this work, capability of fusarium solani f-552 of producing lignocellulose-degrading enzymes in submerged fermentation was investigated. the enzyme cocktail includes hydrolases (cellulases, xylanases, and proteinases) as well as ligninolytic enzymes: manganese-dependent peroxidase (mnp), lignin peroxidase (lip), and laccase (lac). to our knowledge, this is the first report on production of mnp, lip, and lac together by one f. solani strain. the enzyme productions were significantly influenced ... | 2012 | 22488104 |
| antimicrobial activities of rhizomes of polygonatum verticillatum: attributed to its total flavonoidal and phenolic contents. | the current study was undertaken to evaluate the rhizomes of polygonatum verticillatum against various pathogenic bacteria and fungi. broad spectrum antibacterial activity was demonstrated by the crude extract of the plant and its subsequent solvent fractions; predominantly against gram-negative bacteria. mics of the extracts against escherchia coli, salmonella typhi and shigella flexeneri were in the range of 1.5-40 μg/ml, 03-06 μg/ml and 03-40 μg/ml, respectively. the only sensitive gram-posit ... | 2012 | 22459478 |
| cytological karyotyping and characterization of a 410 kb minichromosome in nectria haematococca mpi. | karyotypes of the cucurbit pathogen nectria haematococca mpi (anamorph fusarium solani f. sp. cucurbitae race 1) was studied using the two standard strains atcc18098 and atcc18099. complete separation of all chromosomes was difficult with pulsed field gel electrophoresis due to both the large size and co-migration of chromosomes. in contrast, cytological karyotyping was done successfully with fluorescence microscopy combined with the germ tube burst method for sample preparation to visualize mit ... | 2012 | 22453120 |
| allergens from fusarium solani identified by immunoblotting in asthma patients in iran. | we extracted fusarium solani antigens to evaluate specific anti-f. solani ige in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic healthy subjects (15 men and 7 women). f. solani strains were cultured in sabouraud glucose agar and subjected to cell disruption using the freeze-and-thaw method. the obtained cytoplasmic extracts were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). sensitisation to f. solani antigens has been evaluated i ... | 2012 | 22450199 |
| negative correlation between phospholipase and esterase activity produced by fusarium isolates. | fusarium species have emerged as one of the more outstanding groups of clinically important filamentous fungi, causing localized and life-threatening invasive infections with high morbidity and mortality. the ability to produce different types of hydrolytic enzymes is thought to be an important virulence mechanism of fungal pathogens and could be associated with the environment of the microorganism. here, we have measured the production of two distinct lipolytic enzymes, phospholipase and estera ... | 2012 | 22415116 |
| use of biocides for the control of fungal outbreaks in subterranean environments: the case of the lascaux cave in france. | the lascaux cave in france suffered an outbreak of the fungus fusarium solani in 2001. biocides were applied for three years to control this outbreak. four months after the initial biocide application, a new outbreak appeared in the form of black stains that progressively invaded the cave. the black stains on the ceiling and passage banks were so evident by 2007 that they became one of the cave's major problems. therefore, biocides were used again in 2008. the present study investigated the fung ... | 2012 | 22380699 |
| temperature and moisture effect on spore emission in the fungal biofiltration of hydrophobic vocs. | the effect of temperature and moisture on the elimination capacity (ec), co(2) production and spore emission by fusarium solani was studied in biofilters packed with vermiculite and fed with n- pentane. three temperatures (15, 25 and 35°c) were tested and the highest average ec (64 g m(-3) h(-1)) and lower emission of spores (2.0 × 10(3) cfu m(-3) air) were obtained at 25°c. the effect of moisture content of the packing material indicates that the highest ec (65 g m(-3) h(-1)) was obtained at 50 ... | 2012 | 22375544 |
| phylogenetic analysis of fusarium solani associated with the asian longhorned beetle, anoplophora glabripennis. | culture-independent analysis of the gut of a wood-boring insect, anoplophora glabripennis (coleoptera: cerambycidae), revealed a consistent association between members of the fungal fusarium solani species complex and the larval stage of both colony-derived and wild a. glabripennis populations. using the translation elongation factor 1-alpha region for culture-independent phylogenetic and operational taxonomic unit (otu)-based analyses, only two otus were detected, suggesting that genetic varian ... | 2012 | 26467954 |
| splenectomy as an effective debulking therapy for disseminated mould infection in acute myeloid leukaemia following adjuvant therapy with interferon gamma and liposomal amphotericin. | invasive fungal infection is a major cause of morbidity and mortality in patients receiving treatment for acute myeloid leukaemia (aml). herein, we report a case of a 21 year old woman with an extremely resistant fusarium species that responded to the addition of interferon gamma to her medical therapy, subsequently allowing definitive debulking surgery of her invasive fusarium infection to be undertaken. | 2012 | 24371746 |
| [severe keratomycosis due to fusarium solani induced by a telluric foreign body: about a case in moroccan sahara]. | we report a case of severe keratitis due to fusarium solani in a young man in the sahara in morocco where the climate is arid. this patient reported had a grain of sand in his right eye for a week after a sandstorm. on admission he had a corneal abscess. despite rapid diagnosis and initiation of treatment with available antifungal drugs: amphotericin b and natamycin eye drops, the prognosis worsened and led to the enucleation of the right eye. faced with a suspected eye infection after a microtr ... | 2011 | 24451564 |
| a new furoquinoline alkaloid with antifungal activity from the leaves of ruta chalepensis l. | bioassay-guided separation with an eye toward antifungal activity led to the isolation of the new alkaloid 5-(1̀,1̀-dimethylallyl)-8-hydroxyfuro[2-3-b] quinoline (1) and the known biscoumarin daphnoretin (2) as the active constituents of the chloroform extract obtained from the leaves of ruta chalepensis. the structures of the metabolites were elucidated on the basis of their spectral characteristics (nmr, uv, and ms) and were compared with the literature. the antifungal activity of the isolated ... | 2010 | 22491304 |
| characterization of bacillus isolates of potato rhizosphere from andean soils of peru and their potential pgpr characteristics. | bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (pgp) activities that include biocontrol capacity against some phytopatogenic fungi. potato crops in the andean highlands of peru face many nutritional and phytophatogenic problems that have a significant impact on production. in this context is important to investigate the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising p ... | 2010 | 24031569 |
| phototoxicity ofcitrus jambhiri to fungi under enhanced uv-b radiation: role of furanocoumarins. | extracts ofcitrus jambhiri foliage exposed to and shielded from uv-b radiation were assayed for phytochemical changes and phototoxicity against four fungal pathogens, two of which (fusarium solani andf. oxysporum) are causative agents of root rots and two of which (penicillium italicum andp. digitatum) are associated with fruit rots. conidial pigment mutants of these four fungal species were assayed to determine whether pigments play a role in protecting fungi against plant photosensitizers. exp ... | 1993 | 24248778 |
| chitosanase from the plant pathogenic fungus, fusarium solani f. sp. phaseoli-purification and some properties. | among 162 strains of the genus fusarium tested, 22 strains, mainly from f. solani and f. splendens, formed halos on chitosan-containing agar medium. chitosanase secreted in the culture by the most effective producer, f. solani f. sp. phaseoli suf386, was further investigated. n-acetylglucosamine (glcnac) used as a carbon source was most effective for production of chitosanase. chitosan, a substrate for chitosanase, inhibited cell growth completely and abolished production of chitosanase when use ... | 1993 | 27314774 |
| pea genes associated with non-host disease resistance to fusarium are also active in race-specific disease resistance to pseudomonas. | a given plant species is able to resist most of the potentially pathogenic microorganisms with which it comes in contact. this phenomenon, known as non-host resistance, can be overcome only by a very small number of 'true pathogens' which can use that plant as a host. in some cases, plants have developed mechanisms for overcoming infection by specific races or strains of a true pathogen. this race-specific resistance can be easily manipulated into agronomically important cultivars by plant breed ... | 1987 | 24301193 |
| chitinase in bean leaves: induction by ethylene, purification, properties, and possible function. | ethylene induced an endochitinase in primary leaves of phaseolus vulgaris l. the enzyme formed chitobiose and higher chitin oligosaccharides from insoluble, colloidal or regenerated chitin. less than 5% of the total chitinolytic activity was detected in an exochitinase assay proposed by abeles et al. (1970, plant physiol. 47, 129-134) for ethylene-induced chitinase. in ethylene-treated plants, chitinase activity started to increase after a lag of 6 h and was induced 30 fold within 24 h. exogenou ... | 1983 | 24263941 |
| two cases of fusarium solani (mart) sacc. infection of human finger nails. | fusarium solani, not known to produce onychomycosis, was repeatedly recovered trom two healthy men with'iesions in fingernails. mycelial fragments and microconidia were observed in the diseased nails and the fungus was repeatedly isolated in the cyclo-heximide-free culture medium. the report indicates that many fungi, hitherto considered nonpathogens may still be able to colonize a variety of human tissues. | 2017 | 28193949 |
| antibacterial secondary metabolites from an endophytic fungus, fusarium solani jk10. | extensive chemical investigation of the endophytic fungus, fusarium solani jk10, harbored in the root of the ghanaian medicinal plant chlorophora regia, using the osmac (one strain many compounds) approach resulted in the isolation of seven new 7-desmethyl fusarin c derivatives (1-7), together with five known compounds (8-12). the structures of the new compounds were elucidated by analysis of their spectroscopic data including 1d, 2d nmr, hresi-ms(n) and ir data. the relative configuration of co ... | 2017 | 28392268 |
| chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans. | the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ... | 2017 | 28725151 |
| isolation, identification, optimization, and metabolite profiling of streptomyces sparsus vsm-30. | deep sea sediment samples of bay of bengal (visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. the actinomycetes strain vsm-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. it was identified as streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. response surface methodology regression analysis was carried out to fi ... | 2017 | 28669076 |
| isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria. | annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure. | 2017 | 28507410 |
| application and comparison in biosynthesis and biodegradation by fusarium solani and aspergillus fumigatus cutinases. | in this study, two synthesized cutinase genes from fusarium solani and aspergillus fumigatus were expressed in pichia pastoris x33. the characteristics of these two cutinases were investigated and compared. the results indicated that f. solani and a. fumigatus cutinases hydrolyzed p-nitrophenyl substrates with different carbon chain lengths. a. fumigatus cutinase predominately hydrolyzed p-nitrophenyl butyrate, but f. solani cutinase preferred p-nitrophenyl decanoate. the abilities of polymer sy ... | 2017 | 28673841 |
| nonsteroidal anti-inflammatory drugs (nsaids) inhibit the growth and reproduction of chaetomium globosum and other fungi associated with water-damaged buildings. | indoor mold due to water damage causes serious human respiratory disorders, and the remediation to homes, schools, and businesses is a major expense. prevention of mold infestation of building materials would reduce health problems and building remediation costs. nonsteroidal anti-inflammatory drugs (nsaids) inhibit yeasts and a limited number of filamentous fungi. the purpose of this research was to determine the possible inhibitory activity of nonsteroidal anti-inflammatory drugs (nsaids) on g ... | 2017 | 28795317 |
| engineered humicola insolens cutinase for efficient cellulose acetate deacetylation. | cutinases comprise a family of esterases with broad hydrolytic activity for chain and pendant ester groups. this work aimed to identify and improve an efficient cutinase for cellulose acetate (ca) deacetylation. the development of a mild method for ca fiber surface deacetylation will result in improved surface hydrophilicity and reactivity while, when combined with cellulases, a route to the full recycling of ca to acetate and glucose. in this study, the comparative ca deacetylation activity of ... | 2017 | 28488758 |
| attempt to simultaneously generate three chiral centers in 4-hydroxyisoleucine with microbial carbonyl reductases. | a panel of microorganisms was screened for selective reduction ability towards a racemic mixture of prochiral 2-amino-3-methyl-4-ketopentanoate (rac-amkp). several of the microorganisms tested produced greater than 0.5mm 4-hydroxyisoleucine (hil) from rac-amkp, and the stereoselectivity of hil formation was found to depend on the taxonomic category to which the microorganism belonged. the enzymes responsible for the amkp-reducing activity, apar and fsar, were identified from two of these microor ... | 2017 | 28698052 |
| evaluation of in vitro cytotoxic activity of mono-pegylated stap3 (solanum tuberosum aspartic protease 3) forms. | stap3 is a plant aspartic protease with cytotoxic activity toward a broad spectrum of pathogens, including potato and human pathogen microorganisms, and cancer cells, but not against human t cells, human red blood cells or plant cells. for this reason, stap3 could be a promising and potential drug candidate for future therapies. in this work, the improvement of the performance of stap3 was achieved by means of a modification with peg. the separation of a mono-pegylated stap3 fraction was easily ... | 2014 | 28626641 |
| chlorogenic acid is a fungicide active against phytopathogenic fungi. | plants synthesize diverse types of secondary metabolites and some of them participate in plant protection against pathogen attack. these compounds are biodegradable and renewable alternatives, which may be envisaged for the control of plant pests and diseases. chlorogenic acid (cga) is a phenolic secondary metabolite which accumulates in diverse plant tissues and can be found in several agro-industrial by-products and waste. the aim of this work was to determine whether cga could control the gro ... | 2017 | 28755691 |
| the antibiotic polymyxin b exhibits novel antifungal activity against fusarium species. | the genus fusarium comprises many species, including fusarium oxysporum, fusarium solani, fusarium graminearum and fusarium verticillioides, and causes severe infections in plants and humans. in clinical settings, fusarium is the third most frequent mould to cause invasive fungal infections after aspergillus and the mucorales. f. solani and f. oxysporum are the most prevalent fusarium spp. causing clinical disease. however, few effective antifungal drugs are available to treat human and plant fu ... | 2017 | 28433743 |
| fusarium keratitis in germany. | fusarium keratitis is a destructive eye infection that is difficult to treat and which results in poor outcome. in the tropical and subtropical areas the infection is relatively common and associated with trauma or chronic eye diseases. however, in recent years, an increased incidence has been reported in temperate climate regions. at the german national reference center, we observed a steady increase in case numbers since 2014. here, we present the first german case series of eye infections wit ... | 2017 | 28747368 |
| endophytic fungi harbored in panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease. | endophytic fungi play an important role in balancing the ecosystem and boosting host growth. in the present study, we investigated the endophytic fungal diversity of healthy panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of p. notoginseng. | 2017 | 28701877 |
| genetic diversity and antifungal susceptibility of fusarium isolates in onychomycosis. | fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. onychomycosis caused by fusarium spp. the infection has been increasingly described in the immunocompetent and immunosuppressed hosts. considering onychomycosis is a difficult to treat infection, and little is known about the genetic variability and susceptibility pattern of fusarium spp., further studies are necessary to understand the pathogenesis and better to define th ... | 2017 | 28657120 |
| successful salvage chemotherapy and allogeneic transplantation of an acute myeloid leukemia patient with disseminated fusarium solani infection. | disseminated fusarium infection is associated with high mortality in immunocompromised patients. patients with acute myeloid leukemia (aml) often have an extended duration of neutropenia during intensive induction chemotherapy, consolidation chemotherapy, and hematopoietic stem cell transplantation (sct). there is no consensus regarding management of invasive disseminated fusarium infections in the setting of prolonged neutropenia (tortorano et al., 2014) [1]. we report a case of disseminated fu ... | 2017 | 28794968 |
| scmed7, a sugarcane mediator subunit gene, acts as a regulator of plant immunity and is responsive to diverse stress and hormone treatments. | the mediator complex, is an essential component of the rna polymerase ii general transcriptional machinery in eukaryotes. mediator subunit 7 (med7), a key subunit in the central module of this complex, plays an important role in gene transcriptional regulation. the present study isolated the full-length cdna of the med7 gene of sugarcane, hereby designated as scmed7, which was characterized to harbor a 525-bp open reading frame that is predicted to encode a 174-amino acid protein with a molecula ... | 2017 | 28785867 |
| intracellular siderophore detection in an egyptian, cobalt-treated f. solani isolate using sem-edx with reference to its tolerance. | an egyptian, plant pathogenic fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. the isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. morphology and elemental analysis of the isolate under the investigated co concentrations were studied using scanning electron microscopy (sem) and energy dispersive x-ray microanalysis (edx). the isolate reserved its morphology up to a concentration of 200 ppm. morphological distorti ... | 2017 | 28735319 |
| phytochemistry, antioxidant potential and antifungal of byrsonima crassifolia on soil phytopathogen control. | the use of chemical defensives to control fungal diseases has by consequence to impact negatively over the environment and human health, this way, the use of plant extracts with antifungal properties along with proper cultural management makes viable an alternative plant production control, specially for familiar and organic cultures. the objective of this study was to perform phytochemical and antioxidant analysis of byrsonima crassifolia (canjiqueira) barks and evaluate its antifungal potentia ... | 2017 | 28699968 |
| in vitro nematophagous activity of predatory fungi on infective nematodes larval stage of strongyloidae family. | the main goal of the present research conducted to assess the in vitro activity of the nematophagous fungi duddingtonia flagrans, fusarium solani, verticillium chlamidosporium, and trichoderma harzianum. | 2017 | 28698742 |
| biodegradation of benzo[α]pyrene, toluene, and formaldehyde from the gas phase by a consortium of rhodococcus erythropolis and fusarium solani. | polycyclic aromatic hydrocarbons (pahs) and volatile organic compounds (vocs) are important indoor contaminants. their hydrophobic nature hinders the possibility of biological abatement using biofiltration. our aim was to establish whether the use of a consortium of fusarium solani and rhodococcus erythropolis shows an improved performance (in terms of mineralization rate and extent) towards the degradation of formaldehyde, as a slightly polar voc; toluene, as hydrophobic voc; and benzo[α]pyrene ... | 2017 | 28685193 |
| inhibition of fusarium solani infection in murine keratocytes by lactobacillus salivarius ssp. salivarius jcm1231 culture filtrate in vitro. | to explore the inhibitory activity of lactobacillus salivarius ssp. salivarius jcm1231 (l. salivarius jcm1231) culture filtrate against fusarium solani (f. solani) and its effects on murine keratocytes (mks) infected with f. solani. | 2017 | 28636459 |
| a sugarcane pathogenesis-related protein, scpr10, plays a positive role in defense responses under sporisorium scitamineum, srmv, sa, and meja stresses. | a pathogenesis-related gene, scpr10 , was isolated from sugarcane and its bio-function was characterized, demonstrating that scpr10 was involved in plant defense responses to sporisorium scitamineum , srmv, sa, and meja stresses. plant fungal and viral diseases are the major concerns in sugarcane industry. many anti-fungal and antivirus components, including pathogenesis-related (pr) proteins, have been identified. the pathogenesis-related protein 10 (pr10) is the dominant group in pr families, ... | 2017 | 28634719 |
| flow cytometry is a powerful tool for assessment of the viability of fungal conidia in metalworking fluids. | fungal contamination of metalworking fluids (mwf) is a dual problem in automated processing plants because resulting fungal biofilms obstruct cutting, drilling, and polishing machines. moreover, some fungal species of mwf comprise pathogens such as fusarium solani therefore, the development of an accurate analytical tool to evaluate conidial viability in mwf is important. we developed a flow cytometric method to measure fungal viability in mwf using f. solani as the model organism. to validate t ... | 2017 | 28625992 |
| melanization of fusarium keratoplasticum (f. solani species complex) during disseminated fusariosis in a patient with acute leukemia. | fusarium spp. are recognized as the second most frequently filamentous fungi causing opportunistic infections and particularly important due to the increasing number of immunocompromised patients. f. keratoplasticum (a member of f. solani species complex) is one of the fusarium species commonly associated with human infection, and therefore, studies on the virulence of this fungus are needed. this study aimed to confirm the presence of melanin in f. keratoplasticum from a patient with systemic f ... | 2017 | 28616680 |
| enzymatic hydrolysis of polyester thin films at the nanoscale: effects of polyester structure and enzyme active-site accessibility. | biodegradable polyesters have a large potential to replace persistent polymers in numerous applications and to thereby reduce the accumulation of plastics in the environment. ester hydrolysis by extracellular carboxylesterases is considered the rate-limiting step in polyester biodegradation. in this work, we systematically investigated the effects of polyester and carboxylesterase structure on the hydrolysis of nanometer-thin polyester films using a quartz-crystal microbalance with dissipation m ... | 2017 | 28538100 |
| [fusarioses to fusarium solani in an immunocompetent and immunocompromised diagnosed in military hospital of rabat]. | fusarium are ubiquitous hyalohyphomycoses, usually encountered in the soil. they are the second unusual fungal pathogens after the trichosporon. intertrigo fusarium sp. is a rare achievement. we report a case of intertrigos interorteils in an immunocompetent 45years old and a same case associated with a total onychodystrophy in an immunocompromised 75year-old. laboratory diagnosis has found fusarium solani confirmed with the positivity of a pure culture twice. good progress was noted with terbin ... | 2017 | 28478967 |
| role of pattern recognition receptors in the modulation of antimicrobial peptide expression in the corneal epithelial innate response to f. solani. | fusarium solani (f. solani) keratitis is a potentially sight-threatening fungal infection of the cornea. antimicrobial peptides (amps), such as human β-defensins (hbds) and cathelicidins, essential components of the immune system, likely have a protective role against f. solani keratitis. we examined the role of pattern recognition receptors (prrs), dectin-1, and tlr2 in f. solani-induced modulation of amp expression in vitro. | 2017 | 28460048 |
| non-host resistance: dna damage is associated with sa signaling for induction of pr genes and contributes to the growth suppression of a pea pathogen on pea endocarp tissue. | salicylic acid (sa) has been reported to induce plant defense responses. the transcriptions of defense genes that are responsible for a given plant's resistance to an array of plant pathogens are activated in a process called non-host resistance. biotic signals capable of carrying out the activation of pathogenesis-related (pr) genes in pea tissue include fungal dnase and chitosan, two components released from fusarium solani spores that are known to target host dna. recent reports indicate that ... | 2017 | 28421088 |
| morphological and molecular characterization of fusarium spp. associated with olive trees dieback in tunisia. | dieback and wilting symptoms caused by complex soilborne fungi are nowadays the most serious threatening disease affecting olive trees (olea europaea) in tunisia and presumably in many mediterranean basin countries. fusarium is one of the important phytopathogenic genera associated with dieback symptoms of olive trees. the objective of the present study was to confirm the pathogenicity of fusarium spp. isolated from several olive-growing areas in tunisia. according to the pathogenic test done on ... | 2017 | 28401468 |