Publications

[toxicity of some species of fungi isolated from foodstuffs of polish production in the years 1969-1970. ii. genus aspergillus: a. fumigatus and some other species].		1974	4595352
polyamine, magnesium and ribonucleic acid levels in steady-state cultures of the mould aspergillus nidulans.		1974	4595514
[a quantitative analysis of conidia production in aspergillus nidulans].		1974	4463418
alpha-1,3 glucan synthesis correlated with alpha-1,3 glucanase synthesis, conidiation and fructification in morphogenetic mutants of aspergillus nidulans.		1974	4599936
cytochrome-c reductases from wild-type and mutant strains of aspergillus nidulans.		1974	4600016
a simple and rapid technique for obtaining a high proportion of hybrid cleistothecia in aspergillus nidulans.		1974	4601109
the histopathologic response of mice to aspergillus nidulans: comparison between genetically defined haploid and diploid strains of different virulence.		1974	4601373
a study of the kinetics of hyphal extension and branch initiation of fungal mycelia.		1974	4274556
[metabolites of microorganisms. 143. echinocandin b, a novel polypeptide-antibiotic from aspergillus nidulans var. echinulatus: isolation and structural components].		1974	4613708
regulation of s-amino acids biosynthesis in aspergillus nidulans. role of cysteine and-or homocysteine as regulatory effectors.		1974	4610340
an aspergillus nidulans mutant lacking serine transacetylase: evidence for two pathways of cysteine biosynthesis.		1974	4610342
on the genetic activity of benzimidazole and thiophanate fungicides on diploid aspergillus nidulans.		1974	4601763
the membraneous type of lomasome (membranosome) in the hyphae of aspergillus nidulans.		1974	4602421
regulation of arginine catabolism in aspergillus nidulans.		1974	4602656
[genetic regulation of arginine metabolism in fungi (author's transl)].		1974	4603558
non-disjunction and crossing-over induced by pharmaceutical drugs in aspergillus nidulans.		1974	4604824
regulatory aspects of l-glutamate transport in aspergillus nidulans.	wild-type cells of aspergillus nidulans synthesize a transport system which appears to be specific for l-glutamate and l-aspartate. the system is energy dependent and concentrates l-glutamate at least 60-fold. in cells grown in the presence of 1% sucrose, l-glutamate uptake activity is regulated by ammonium control, although it is not certain whether this is at the level of transcription or translation. mutants that are insensitive to ammonium control of certain other unrelated systems, e.g., ni ...	1974	4605030
the production of invertase in aspergillus nidulans with reference to the effects of glucose and sucrose.		1974	4605317
the regulation of aryl sulphatase in aspergillus nidulans.		1974	4605323
[effect of sporulation and culture conditions on the synchrony of nuclear division of germinating aspergillus nidulans conidia].		1974	4607895
[ultrastructure of the nucleus-associated body ("centriolar plaque", "mtoc", "kce") during the interphase in the conidia of aspergillus nidulans].		1974	4618941
cold-sensitive mutants in aspergillus nidulans. ii. mutations affecting ribosome production.		1974	4617158
factors affecting glucose uptake in aspergillus nidulans.		1974	4615063
comparison of the activities of nitrogen mustards according to induced mitotic crossing over in a diploid strain of aspergillus nidulans.		1974	4616347
mutants of the pentose phosphate pathway in aspergillus nidulans.	mutants of the pentose phosphate pathway have been isolated in aspergillus nidulans. these fail to grow on a variety of carbohydrates that are catabolized through the pentose phosphate pathway. they also grow poorly on nitrate and nitrite as sole nitrogen sources. the pentose phosphate pathway mutations have been assigned to two unlinked genes. mutants with lesions in the pppb locus have reduced activities of four enzymes of the pentose phosphate pathway, of glucose-phosphate isomerase, and of m ...	1974	4591946
mutagenic activity of n-methyl-n'-nitro-n-nitrosoguanidine (ntg) and n-methyl-n-nitrosourea (nmu) in aspergillus nidulans.		1974	4599103
a theoretical mechanism of spirochete locomotion.		1974	4616991
cold-sensitive mutants in aspergillus nidulans. i. isolation and general characterisation.		1974	4617164
the transport of ammonium and methylammonium in wild type and mutant cells of aspergillus nidulans.		1974	4614070
new proline suppressor mutants in aspergillus nidulans.		1974	4597553
studies on the role of the area gene in the regulation of nitrogen catabolism in aspergillus nidulans.	mutants of apergillus nidulans with lesions in a gene, area (formerly called amdt), have been isolated by a variety of different selection methods. the area mutants show a range of pleiotropic growth responses to a number of compounds as sole nitrogen sources, but are normal in utilization of carbon sources. the levels of two amidase enzymes as well as urease have been investigated in the mutants and have been shown to be affected by this gene. most of the area mutants have much lower amidase-sp ...	1975	52352
regulation of mannitol-1-phosphate dehydrogenase in aspergillus nidulans.		1975	163669
a mutant of aspergillus nidulans defective in nad-linked glutamate dehydrogenase.	a mutation leading to partial loss of nad-linked ("catabolic') glutamate dehydrogenase does not affect the regulation of ammonium-repressible activities in aspergillus nidulans. this mutation has been used to show that nad-linked glutamate dehydrogenase does not normally participate in ammonium assimilation. a mutation leading to loss of nadp-linked ("anabolic') glutamate dehydrogenase has been used to show that nadp-linked glutamate dehydrogenase is not normally involved in glutamate catabolism ...	1975	172777
sexual differentiation in aspergillus nidulans: the requirement for manganese and the correlation between phosphoglucomutase and the synthesis of reserve material.	aspergillus nidulans was completely devoid of fruit bodies when grown on manganese deficient cultures. this result was shown earlier to be due to a lack of alpha-1,3 glucan in the cell wall. several enzymes of carbon and nitrogen metabolism were investigated in an attempt to explain the absence of this reserve material. synthesis of glucose-6-phosphate dehydrogenase, phosphoglucoisomerase and aldolase, were not strongly affected by manganese deficiency. however, phosphoglucomutase showed only 60 ...	1975	173248
three genes determine the carboxin sensitivity of mitochondrial succinate oxidation in aspergillus nidulans.		1975	178574
the structural gene for nadp l-glutamate dehydrogenase in aspergillus nidulans.	a total of 41 mutants lacking nadp l-glutamate dehydrogenase (nadp-gdh) activity have been studied. all the mutations were located at the gdha locus within 0-1% recombination of gdhai. two mutants, gdhai and gdha2, out of five examined, produced cross-reacting material which neutralized nadp-gdh anti-serum. the mutant gdha9 has altered km values for all five substrates: ammonium, alpha-ketoglutarate, l-glutamate, nadph and nadp. the mutant gdha20 had temperature-sensitive growth, abnormal ammoni ...	1975	234511
compartmentation and regulation of fungal metabolism: genetic approaches.		1975	129029
pyrimidine biosynthesis in aspergillus nidulans. isolation and characterisation of mutants resistant to fluoropyrimidines.	mutants resistant to 5-fluorouracil, 5-fluorouridine and 5-fluorodeoxyuridine have been selected in aspergillus nidulans. growth tests combined with genetic analysis showed that mutations conferring resistance to fluoropyrimidines could occur in at least seven genes. three of these fule, fulf and fura were concerned with either the uptake of pyrimidines or their conversion to uridine monophosphate. the other four genes did not affect these functions. mutations in fula probably confer resistance ...	1975	127929
in vitro restoration of nitrate reductase: investigation of aspergillus nidulans and neurospora crassa nitrate reductase mutants.	extracts of aspergillus nidulans wild type (bi-1) and the nitrate reductase mutant niad-17 were active in the in vitro restoration of nadph-dependent nitrate reductase when mixed with extracts of neurospora crassa, nit-1. among the a. nidulans cnx nitrate reductase mutants tested, only the molybdenum repair mutant, cnxe-14 grown in the presence of 10-minus 3 m na2 moo4 was active in the restoration assay. aspergillus extracts contained an inhibitor(s) which was measured by the decrease in nadph- ...	1975	123779
a modified minimal medium for aspergillus nidulans.		1975	328
carbon catabolite repression in aspergillos nidulans.		1975	168071
kinetics and morphology of glucose-limited cultures of moulds grown in a chemostat and on solid media.	the affinity (ks value) of geotrichum candidum for glucose determined from chemostate cultures was ca. 1 mg/1. ks values for glucose was also estimated from the radial growth rates of colonies of g. candidum and neurospora crassa grown on media solidified with agar or silica gel. an assessment is made of the use of colony radial growth rate to determine substrate affinities. the length of apical and intercalary hyphal comparte ments, internode length and the diameter of leading hyphaat the margi ...	1975	168829
analysis of results of catheter tip cultures in open-heart surgery patients.	a statistical analysis of 628 consecutive catheter tip cultures is presented. all were from patients undergoing open-heart surgery. the previously noted effects of early removal are shown to be significant. the possible effects of stasis, flushing, handling, and place of insertion are discussed. the unresolved significance of isolations of aerobic sporing bacilli is noted, and a decreasing incidence of postoperative infective endocarditis in the same group of patients is an encouraging sign.	1975	235801
the regulation of nitrate reductase in the fungus aspergillus nidulans.		1975	241670
enzymology and genetic regulation of a cyclic nucleotide-binding phosphodiesterase-phosphomonoesterase from aspergillus nidulans.	a cyclic nucleotide-binding phosphohydrolase that possesses both a phosphomonoesterase and a phosphodiesterase catalytic function has been partially purified from aspergillus nidulans. the enzyme hydrolyzes both p-nitrophenylphosphate and bis-(p-nitrophenyl)-phosphate. o'-nucleoside monophosphates are the best physiological phosphomonesterase substrates but 5'- and 2'-nucleoside monophosphates are also hydrolyzed. the enzyme catalyzes the hydrolysis of adenosine 5'-triphosphate, adenosine 5'-dip ...	1975	241743
role of organic phosphates in the formation of phosphatases in aspergillus nidulans.		1975	767255
mitochondrial dna from aspergillus nidulans.		1975	773682
subcellular mislocation of cysteine synthase in a cysteine auxotroph of aspergillus nidulans.		1975	773683
ornithine decarboxylase activity in germinating conidia of aspergillus nidulans.		1975	773695
mitotic mutants of aspergillus nidulans.		1975	773766
three-marker extranuclear mitochondrial crosses in aspergillus nidulans.	two- and three-point extranuclear crosses have been carried out via heterokaryons involving the three extranuclear mitochondrial markers of aspergillus nidulans: (olia1), (cs67) and (cama112). all three markers appear to be located on a single functional mitochondrial genome. recombination between all three pairs of extranuclear markers appears to be equally frequent, suggesting a lack of genetic linkage. an important feature of these results is the variable and often marked non-equality of freq ...	1975	765725
[thermosensitive lethal mutants in aspergillus nidulans].		1975	785156
transmission and recombination of extranuclear genes during sexual crosses in aspergillus nidulans.	three extranuclear mitochondrial mutations in aspergillus nidulans, (olia1), (cama1) and (cs67), were used as markers in sexual crosses to provide information on the frequencies of transmission and recombination of the mitochondrial genome. any individual perithecium contained ascospores of only one extranuclear genotype. using mono-, bi- and trifactorial crosses it was found that all three markers could be recovered from the progeny, although the transmission frequencies were different for each ...	1975	765748
acquired resistance to benzimidazole derivatives.		1975	773644
altered instability due to genetic changes in a duplication strain of aspergillus nidulans.		1975	767217
counterimmunoelectrophoresis in the diagnosis of mycetoma and its sensitivity as compared to immunodiffusion.	counterimmunoelectrophoresis used for the diagnosis of mycetoma was found superior to immunodiffusion (id) especially when using neat serum and 1:2 dilution (p=less than 0.0001 with neat serum; and less than 0.01 with 1:2 dilution). it is recommended for routine use in mycology laboratories for following up patients on treatment and for confirming the diagnosis of sera that are weakly positive by id.	1975	817400
a cis-dominant regulatory mutation affecting enzyme induction in the eukaryote aspergillus nidulans.		1975	1089206
increased fusion frequency of aspergillus nidulans protoplasts.		1975	1089544
glucose uptake by aspergillus nidulans, purification and properties of glucose binding protein.		1975	1089548
hemolytic, cytotoxic and complement inactivating properties of extracts of different species of aspergillus.	some of the biological properties of saline extracts of the mycelia of several species of the aspergillus genus, namely, a. fumigatus, a. flavus, a. niger, a. nidulans, a. parasitucus and a. glaucus, were studied. only the extract prepared from a. fumigatus was found to be hemolytic for sheep red blood cells. in contrast, all the extracts with the only exception of that of a. glaucus, had cytotoxic effects on vero cells. both, the hemolytic and cytotoxic constituents of the extracts were removab ...	1975	813147
a gene cluster in aspergillus nidulans with an internally located cis-acting regulatory region.	work reported here on the fungus aspergillus nidulans has provided the first definitive demonstration of operon-type organisation in an eukaryote genome. it has been shown that the prna and prnb genes concerned with proline metabolism form a gene cluster with the regulatory region lying between the two putative structural genes prna and prnb. regulatory mutations (prnd) probably leading to relief of carbon catabolite repression, map in between prna and prnb and are cis-dominant with respect to b ...	1975	1089903
initiator constitutive mutation with an 'up-promoter' effect in aspergillus nidulans.	a mutation leading to strong constitutivity for a uric acid-xanthine permease in the lower eukaryote aspergillus nidulans has been found to be tightly linked to the putative structural gene whose expression it controls in the cis configuration. in addition, it increases the maximal induced level of transport 2.5-fold. this is the first demonstration of an initiator or promoter mutation in an eukaryote.	1975	1089904
effect of l-histidine on the catabolism of nitrogenous compounds in aspergillus nidulans.		1975	1094095
nitrate utilization and growth in biotin-deficient aspergillus nidulans.	a marked increase in the cellular synthesis accompanied by a decrease in the total fatty acid content was observed when aspergillus nidulans was grown on nh4no3 as a sole nitrogen source, in the medium containing avidin. because of the increased uptake of nh4(plus), the level of nh4(plus), was lowered in the medium; as a result there is early uptake and assimilation of nitrate by a biotin-deficient culture as compared with the normal culture of a. nidulans. at about 17mm concentration, nh4(plus) ...	1975	1097067
homolanthionine in fungi: accumulation in the methionine-requiring mutants of aspergillus nidulans.	homolanthionine, a higher homologue of cystathionine, was found to accumulate in the mutants of aspergillus nidulan impaired in the synthesis of methionine from homocysteine. the additional introduction of mutation resulting in a block at cystathionine gamma-synthase but not at cystathionine beta-synthase abolishes accumulation of both homolanthionine and cystathionine. this suggests a possible participation of cystathionine gamma-synthase in homolanthionine synthesis.	1975	1101592
mutants affecting histidine utilization in aspergillus nidulans.		1975	1102388
an easy way of obtaining aspergillus nidulans haploids in the parasexual cycle using n-glycosyl polifungin.		1975	1102390
mutations affecting mitotic recombination frequency in haploids and diploids of the filamentous fungus aspergillus nidulans.	a haploid strain of asp. nidulans with a chromosome segment in duplicate (one in normal position on chromosome i, one translocated to chromosome ii) shows mitotic recombination, mostly by conversion, in ade in a frequency slightly higher than in the equivalent diploid. a method has been devised, using this duplication, for the selection of rec and uvs mutations. six rec mutations have been found which decrease recombination frequency in the haploid. one mutation selected as uv sensitive showed a ...	1975	1102911
proceedings: fungicides causing mitotic segregation in aspergillus diploids.		1975	1102965
formation of 2,4-dihydroxybenzoic acid & resorcinol as intermediates in the degradation of salicylic acid by aspergillus nidulans.		1975	1107218
the use of analytical electron microscopy in the study of three species of aspergillaceae.	the combination of electron beam scanning and x-ray detection allows to use the scanning electron probe microanalyzer as a sensitive microscope to elemental constitution. this made it possible to investigate three species of the aspergillus nidulans group: a. nidulans, a. unguis, a. variecolor. qualitative differences were found between the three species. the application of microanalysis is regarded as a useful addition to more conventional methods used in histochemical studies. the advantages o ...	1975	787898
cytoplasmic and nuclear mutations to chloramphenicol resistance in aspergillus nidulans.	two chloramphenicol resistance mutations out of 123 tested in aspergillus nidulans are inherited extranuclearly as judged by transmissibility in heterokaryons, lack of segregation at meiosis, and independent segregation from all of the eight nuclear linkage groups. they do not recombine with each other. however, experiments in collaboration with g. turner and r.t. rowlands show that they do recombine with cytoplasmic mutations to oligomycin resistance (rowlands and turner, 1973) and cold-sensiti ...	1975	1102919
amide utilization in aspergillus nidulans: evidence for a third amidase enzyme.	a mutation in a gene designated gmda has been found to lead to loss of ability of aspergillus nidulans to use benzamide, phenylacetamide and several other amides as sole nitrogen sources for growth. the gmda1 lesion results in low levels of an enzyme, called the general amidase, which has acitivity for a wide range of amide substrates. this enzyme is reressed by certain nitrogen-containing metabolites, including ammonium, but is probably not regulated by induction or by carbon catabolite repress ...	1975	1104771
studies of partially repressed mutants at the tama and area loci in aspergillus nidulans.	mutants, designated tamar, have been isolated on the basis of simultaneous resistance to toxic analogues thiourea, aspartate hydroxamate and chlorate with l-alanine as the sole nitrogen source. tamar mutants are also resistant to methylammonium. this resistance of tamar mutants is correlated with partially repressed activity of a number of enzyme and transport systems regulated by ammonium. furthermore, tam-ar mutants have low nadp-glutamate dehydrogenase (nadp-gdh) activity and also efflux ammo ...	1975	1105154
the inheritance of penicillin titre in wild-type isolates of aspergillus nidulans.	in a sample of 52 wild-type isolates of aspergillus nidulans, penicillin titre ranged from 0-0 to 14-4 units/ml. these differences in titre were under genetic control. most of the variation between isolates was attributable to differences between heterokaryon-compatibility groups although significant differences were also found within groups. genetic variation for penicillin titre was observed among the progeny in four of seven crosses between wild-type isolates. in these four crosses the variat ...	1975	803549
the design of fermentation and biological assay procedures for assessment of penicillin production in populations of aspergillus nidulans.		1975	805120
the fine structure of the branched alpha-d-glucan from the blue-green alga anacystis nidulans: comparison with other bacterial glycogens and phytoglycogen.	the fine structure of the glycogen from the blue-green alga anacystis nidulans has been examined. after selective hydrolysis of all (1 yields 6)-alpha-d linkages by a bacterial isoamylase, the resulting mixture of linear chains was subjected to gel permeation chromatography. for purposes of comparison, the glycogens from escherichia coli and arthrobacter sp., amylopectin, phytoglycogen from sweet corn, and shell-fish glycogen were treated similarly. the profiles of the unit chains of a nidulans ...	1975	806344
labeling the deoxyribonucleic acid of anacystis nidulans.	analysis of cell-free extracts of anacystis nidulans disclosed the absence of both thymidine phosphorylase (ec 2.4.2.4) and thymidine kinase (ec 2.7.1.21) activities. thymine and thymidine were incorporated inefficiently by intact cells of a. nidulans either in the presence or absence of deoxyguanosine (250 mug/ml). deoxythymidine monophosphate incorporation was also inefficient. radioactive deoxyadenosine, at a minimally toxic level (3 mug/ml), was incorporated effectively into the deoxyribonuc ...	1975	809413
sequence studies on 16s ribosomal rna from a blue-green alga.	the 16s ribosomal rna of the blue green alga anacystis nidulans has been characterized in terms of the oligomers generated by digestion with t1 ribonuclease. a. nidulans by this criterion is definitely a procaryote; being no more distant from bacilli or enterics than the latter two are from one another. a. nidulans appears to be somewhat more closely related to the bacilli than to the enterics.	1975	813006
pyrimidine biosynthesis in aspergillus nidulans: isolation and preliminary characterisation of auxotrophic mutants.	113 pyrimidine auxotrophs, unable to synthesise ump have been selected in aspergillus nidulans. these mutants can be classified by complementation into eight groups, and genetic analysis has shown that five loci are involved. one complex locus consists of the mutually complementing pyra, pyrb and pyrc groups, as well as the cis-dominant pyrn group, members of which do not complement with members of the a, b or c groups. pyra mutants have been shown to lack cpsase-ur, pyrb and pyrc mutants have b ...	1975	1102932
mutations which affect amino acid transport in aspergillus nidulans.	mutants deficient in amino acid transport (aau), and unable to utilize l-glutamate as a sole carbon and nitrogen source, have been isolated. there are four unlinked genes involved: aaua, aaub, aauc and aaud. the transport levels of certain amino acids, and the growth characteristics on certain nitrogen and carbon sources and toxic amino acid analogues, indicate that: aauai has defective transport of acidic amino acids; aaubi has defective transport of acidic and neutral amino acids; aauci and aa ...	1975	1089752
autolytic enzymes in hyphae of aspergillus nidulans: their action on old and newly formed walls.	walls, purified from hyphae of the ascomycete aspergillus nidulans, autolyzed on incubation and liberated glucose, mannose, galactose, n-acetylglucosamine, and soluble oligosaccharides. digestion proceeded at linear rates until approximately 3% of the wall polymers had been hydrolyzed and then slowed markedly. the change in the rate of autolysis was not due to loss of enzyme activity but was caused by the disappearance of a fraction of the wall which was highly susceptible to digestion. radioact ...	1975	1090573
keratomycosis in lucknow.		1975	1092323
reciprocal translocations and translocation disomics of aspergillus and their use for genetic mapping.	two new techniques are described for genetic mapping of reciprocal translocations in a. nidulans, which can be used to locate centromeres and meiotically unlinked markers. they both make use of unbalanced disomics from heterozygous translocation crosses. these are mainly hyperhaploids of two classes: either typical-looking n + 1 with a normal chromosome in addition to a haploid set containing the translocation, or translocation disomics. when large chromosome segments are involved, such disomics ...	1975	1092596
the effect of chloral hydrate upon mitosis in aspergillus nidulans.		1975	1097582
quantitative tissue invasion of the murine brain as a phenotypic marker of strain virulence in aspergillus nidulans.	in this study the extent of fungal invasion in tissues of the target organs (brain, heart and kidney) has been quantitated histologically in dba/2j mice inoculated intravenously with 3 strains of aspergillus nidulans of different virulence. this was done to determine the relation between tissue invasion and strain virulence as determined by time-mortality bioassay. it was found that quantitatively tissue invasion by a. nidulans was target organ specific. relative tissue invasion within each of t ...	1975	1099700
enzymatic lesions in methionine mutants of aspergillus nidulans: role and regulation of an alternative pathway for cysteine and methionine synthesis.	in aspergillus nidulans the pathway involving cystathionine formation is the main one for homocysteine synthesis. mutants lacking cystathionine gamma-synthase or beta-cystathionase are auxotrophs suppressible by: (i) mutations in the main pathway of cysteine synthesis (cysa1, cysb1, and cysc1), (ii) mutations causing stimulation of cysteine catabolism (su101), and (iii) mutations in a presumed regulatory gene (suameth). a relative shortage of cysteine in the first group of suppressors causes a d ...	1975	1102536
sexual differentiation in aspergillus nidulans: the requirement for manganese and its effect on alpha-1,3 glucan synthesis and degradation.	aspergillus nidulans was grown on media with added amounts of manganese ranging from 0--2.5 mum. manganese deficiency prevented cleistothecium development, although good vegetative growth was retained. subsequent analysis of the mycelium produced under mn2+ deficient growth revealed that alpha-1,3 glucan, the man carbon and energy source for fructification, was virtually absent from the cell wall. several enzymes related to cell wall composition were investigated. beta-1,3 glucanase, and very re ...	1975	1106342
an unstable strain of aspergillus foetidus segregating proline auxotrophs.	two basic colony types have been obtained through single conidial isolation from the bode strain of aspergillus foetidus as well as from mutants of this unstable strain. type i is prototrophic whereas type ii is an auxotroph requiring proline. when a type i strain is grown on complex medium it gradually becomes overwhelmed by type ii sectors of growth. however, essentially pure cultures of type i can be maintained on minimal medium (lacking proline). the yield of glucoamylase from type ii cultur ...	1975	1147611
mycotoxin-producing potential of mold flora of dried beans.	to evaluate the potential for mycotoxin production by molds in dried beans, the mold flora of 114 samples was determined both before and after surface disinfection of the beans with 5% naocl. surface disinfection substantially reduced mold incidence, indicating that contamination was mainly on the surface. the flora, both before and after disinfection, was dominated by species of the aspergillus glaucus group, the toxicogenic species a ochracues, penicillium cyclopium, and p. viridicatum, and sp ...	1975	1168442
hybridization and selection for increased penicillin titre in wild-type isolates of aspergillus nidulans.	repeated hybridization and selection among wild-type isolates produced strains of aspergillus nidulans with increased penicillin titre. four independent selection lines were established, each originating from a sexual cross between two different heterokaryon-incompatible wild-type isolates. in each generation, two selected high-titre sister strains were crossed to produce the next generation. an initial increase in titre was obtained in each line, but after four or five generations of selection ...	1975	1107473
genetic control of chromosome instability in aspergillus nidulans as a mean for gene amplification in eukaryotic microorganisms.	a haploid strain of aspergillus nidulans carrying i-ii duplication homozygous for the leaky mutation ade20 shows impreved growth on minimal medium. the duplication, though more stable than disomics, still shows instability. several methods were used for detecting genetic control of improved stability. (a) visual selection, using a duplicated strain which is very unstable due to uv sensitivity, (ade20, bial/dp ya2; uvsb). one stable strain showed a deletion (or a lethal mutation?) distal to bia o ...	1975	1107799
cysteine biosynthesis in aspergillus nidulans.	the existence of two postulated pathways of anabolic cysteine biosynthesis in aspergillus midulans was investigated. no activities of the postulated pathway involving s-sulfocysteine as intermediate have been detected. investigations on cyteine and methionine requiring mutants revealed independent regulation of o-acetylserine sulfhydrylase by endogeneous cysteine and methionine pools. the reaction catalysed by o-acetylserine sulfhydrylase is postulated as the only anabolic pathway of cysteine bi ...	1975	1108603
hybridization and selection for increased penicillin titre in wile-type isolates of aspergillus nidulans.	repeated hybridization and selection among wild-type isolates produced strains of aspergillus nidulans with increased penicillin titre. four independent selection lines were established, each originating from a sexual cross between two different heterokaryon-incompatible wild-type isolates. in each generation, two selected high-titre sister strains were crossed to produce the next generation. an initial increase in titre was obtained in each line, but after four or five generations of selection ...	1975	1107474
the inheritance of penicillin titre in crosses between lines of aspergillus nidulans selected for increased productivity.	selection for increased penicillin production among the progeny of pairwise crosses between wild-type isolates of aspergillus nidulans resulted in the production of high-titre strains. three crosses were made between strains with increased titre derived from independent selection lines. significant genetic variation was found among the progeny of each cross, indicating that different genes for increased titre had been selected in each line. gene action was additive in each cross. renewed selecti ...	1975	1107475
metabolism of delta-aminolevulinic acid in red and blue-green algae.	delta-aminolevulinic acid was incorporated in vivo into c-phycocyanin and b-phycoerythrin in two species of the rhodophyta (cyanidium caldarium, porphyridium cruentum) and three species of the cyanophyta (anacystis nidulans, plectonema boryanum, phormidium luridum). amino acid analysis of phycocyanin-(14)c from c. caldarium cells which had been incubated with delta-aminolevulinate-4-(14)c showed that 84% of the radioactivity incorporated was present in the phycocyanobilin chromophore and less th ...	1975	16659103
uv mutagenesis in inhibitor depleted conidia of aspergillus nidulans.	uv treated conidia of a strain of aspergillus nidulans (meth gl. bial) depleted of germination inhibitory substances have been examined for inactivation and mutation induction at groups of suppressor gene loci defining three classes of methionine revertants. an exponential decline in the colony forming ability and quadratic increase in mutation frequency (for each class of revertant) as the incident dose increased were observed. the induced mutation frequency for each class and the loss of colon ...	1976	14997599
presence of histones in aspergillus nidulans.	five major histone proteins have been extracted from chromatin isolated from purified nuclei of the fungus, aspergillus nidulans. these proteins had chromatographic properties which were similar to reference calf thymus histones and were purified to electrophoretic homegeneity by gel chromatography of bio-gel p10, bio-gel p60, and sephadex g-100. electrophoresis of these proteins in three different systems (urea-starch, urea-acetic acid polyacrylamide, and discontinuous sds polyacrylamide) showe ...	1976	799641
tumor induction by a single subcutaneous injection of sterigmatocystin in newborn mice.	sterigmatocystin, a mycotoxin produced by aspergillus versicolor, aspergillus sydowi, aspergillus nidulans, and a species of bipolaris, was given to newborn balb/c x dba/2f1 (hereafter referred to as cd2f1) mice by a single s.c. administration in 1% gelatin suspension. in an acute toxicity study, the maximum tolerated dose of sterigmatocystin was 5 mug/g body weight. in a chronic study, a single s.c. injection of 5, 1, or 0.5 mug/g body weight gave rise to high incidences of lung and liver adeno ...	1976	1268822
cytochrome abnormalities and cyanide-resistant respiration in extranuclear mutants of aspergillus nidulans.	the cytochrome spectra of two extranuclear mutants of aspergillus nidulans and the double-mutant recombinant formed from them have been examined both at room temperature and at the temperature of liquid n2 and compared with those of the wild-type strain. the oligomycin-resistant, slow growing mutant contained an increased amount of cytochrome c without any loss of cytochromes b and a,a3. the cold-sensitive mutant, apparently normal when grown at 37 c, showed an increased amount of cytochrome c a ...	1976	1107321