Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| partial characterization of the gene coding for subunit iv of soybean mitochondrial nadh dehydrogenase. | by using the spinach chloroplast atpe gene (epsilon-subunit coding gene) as a probe we have isolated, from a soybean mitochondrial dna library, a sequence containing a 405 base-pairs (bp) open-reading frame (orf). this orf, which is unique in the soybean mitochondrial genome, is probably part of an exon of the gene coding for subunit iv of the nadh dehydrogenase complex. the predicted protein shows 42% sequence similarity with the c-terminal region the aspergillus nidulans nad4 protein. the gene ... | 1989 | 2743433 |
| keratinolytic fungi of wadi qena in egypt. | forty-six soil samples collected from different sites of wadi qena were examined for keratinophilic fungi using the hair baiting technique. thirty-two species in addition to one variety of each of a. nidulans and a. flavus which belong to eighteen genera were recovered. aspergillus, chrysosporium, penicillium, microsporum and fusarium were the most frequent genera developed from baited soils. | 1989 | 2707686 |
| aspergillus nidulans as a test organism for the detection of chemically-induced mitotic crossing-over and chromosome malsegregation. | the genetic systems developed in the mould aspergillus nidulans to study the chemical induction of mitotic genetic segregation were used to investigate the mechanism of aneuploidy induction by the main benzene hydroxy metabolites. detailed genetic analysis of mitotic segregants produced by individual colonies revealed the simultaneous occurrence of both whole chromosome segregants and mitotic cross-overs, i.e. a pattern not compatible with the induction of numerical abnormalities as the primary ... | 1989 | 2698601 |
| [relation of bronchial asthma and allergic bronchopulmonary aspergillosis]. | an allergological examination was conducted in 30 out of 83 (36.07%) patients showing symptoms of bronchial asthma. it was found that skin hypersensitivity to aspergilla allergens coupled with a high serum ige level. the appropriateness of such affection referral to a separate nosological entity (allergic bronchopulmonary aspergillosis) is under discussion. | 1989 | 2697785 |
| on the mechanisms of induced aneuploidy in aspergillus nidulans and validation of tests for genomic mutations. | 1989 | 2696976 | |
| immunochemical assay applied to mycotoxin biosynthesis: elisa comparison of sterigmatocystin production by aspergillus versicolor and aspergillus nidulans. | conventional thin layer and instrumental methods for analyzing mycotoxins and their precursors are time-consuming and make the investigation of mycotoxin biosynthesis particularly difficult. as an alternative, sensitive enzyme-liked immunosorbent assays (elisas) can be utilized to analyze for these compounds. in this report, sterigmatocystin production in test tube cultures of aspergillus versicolor atcc 18643 and aspergillus nidulans atcc 32610 were compared using competitive elisa. polyclonal ... | 1989 | 2693965 |
| characterization of purine hydroxylase i from aspergillus nidulans. | purine hydroxylase i from aspergillus nidulans was purified 850-fold. the purified preparations exhibited the spectral and catalytic properties, including broad specificity for oxidizing and reducing substrates, typical of molybdenum/flavin/iron-sulphur-containing hydroxylases (oxotransferases). | 1989 | 2693595 |
| genetic transformation of the filamentous yeast, trichosporon cutaneum, using dominant selection markers. | an efficient transformation system for the filamentous yeast, trichosporon cutaneum, has been developed. transformation was obtained with plasmids carrying either the escherichia coli hygromycin b phosphotransferase-encoding gene (hph) or the streptoalloteichus hindustanus phleomycin-resistance gene (ble), as dominant selection markers. expression of both resistance-conferring genes was controlled by the gpd promoter and the trpc terminator, from aspergillus nidulans. the transformation frequenc ... | 1989 | 2693213 |
| beta-n-acetylglucosaminidase from aspergillus nidulans which degrades chitin oligomers during autolysis. | a hexosaminidase from autolyzed cultures of aspergillus nidulans was purified 196 fold and characterized as a beta-n-acetylglucosaminidase (ec 3.2.1.30). the enzyme has a mw of 190000, a pi of 4.3, and optimum ph of 5.0 and is unstable at temperatures above 50 degrees c. the enzyme is a glycoprotein with 19.5% sugars, mannose being the principal component. it binds strongly to chitin. the enzyme hydrolyzes different substrates. the ki with the competitive inhibitor 2-acetamido-2-deoxy-d-gluconol ... | 1989 | 2693201 |
| rna-mediated genetic transformation in aspergillus nidulans. | we developed a model-system for correcting genetic alterations of an aspergillus nidulans strain (ribo, paba, bio, w, acr) by treating protoplasts with total rna extracted from another a. nidulans strain bearing wild type alleles for the same genetic markers. the results revealed the occurrence of a true genetic transformation. the phenomenon was rna-dependent since it was abolished by pancreatic ribonuclease treatment. the term retrotransformation is proposed since the rna messages artificially ... | 1989 | 2692827 |
| rhizoxin resistant mutants with an altered beta-tubulin gene in aspergillus nidulans. | rhizoxin and ansamitocin p-3 (a maytansinoid compound), potent inhibitors of mammalian brain tubulin assembly, inhibit growth of a variety of fungi including aspergillus nidulans. mutants of a. nidulans, bena10 which is a benomyl resistant beta-tubulin gene mutant and tuba1 which is a benomyl supersensitive alpha-tubulin gene mutant, were both sensitive to rhizoxin and ansamitocin p-3 to the same extent as wild-type strains. we isolated 18 rhizoxin resistant mutants of a. nidulans. all of these ... | 1989 | 2691873 |
| cloning and characterization of the trpc gene from an aflatoxigenic strain of aspergillus parasiticus. | the trpc gene in the tryptophan biosynthetic pathway was isolated from an aflatoxigenic aspergillus parasiticus by complementation of an escherichia coli trpc mutant lacking phosphoribosylanthranilate isomerase (prai) activity. the cloned gene complemented an e. coli trpc mutant deficient in indoleglycerolphosphate synthase (igps) activity as well as an aspergillus nidulans mutant strain that was defective in all three enzymatic activities of the trpc gene (glutamine amidotransferase, igps, and ... | 1989 | 2690735 |
| a comparative study on ethanol and acetaldehyde as inducers of chromosome malsegregation in aspergillus nidulans. | the activity of ethyl alcohol and acetaldehyde on mitotic chromosome segregation and conidial germination in aspergillus nidulans was studied. ethanol effectively induced malsegregation in a narrow range of concentrations (4.5-5.5%, v/v) and was inactive at doses which arrested conidial germination (above 6%). the same bell-shaped dose-response curve was shown by the spindle poison chloral hydrate, which was active in the range 6-10 mm. acetaldehyde displayed a diphasic dose-response curve. gene ... | 1989 | 2689879 |
| a gene transfer system based on the homologous pyrg gene and efficient expression of bacterial genes in aspergillus oryzae. | a homologous transformation system for aspergillus oryzae is described. the system is based on an a. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrg) and the vector pao4-2, which contains a functional a. oryzae pyrg gene as selection marker. transformation of the a. oryzae pyrg mutant with circular pao4-2 resulted in the appearance of pyr+ transformants at a frequency of up to 20 per micrograms of dna, whereas with linear pao4-2 up to 200 transformants per micrograms dna we ... | 1989 | 2688930 |
| aspergillus findings in aids patients suffering from cryptococcosis. | because of the known pathogenicity of cryptococcus neoformans and of aspergilli depending on defined but different immunodeficiencies of the host, the evaluation of their simultaneous cultural detection in specimens of the respiratory tract of aids patients is of epidemiological, diagnostic, pathogenetic and therapeutic interest. in 10 out of 15 aids patients the following species of the genus aspergillus could be isolated either once or repeatedly during the course of cr. neoformans infections ... | 1989 | 2685598 |
| isolation and analysis of the acetate regulatory gene, facb, from aspergillus nidulans. | the facb gene of aspergillus nidulans is thought to be involved in acetate induction of enzymes required for acetate utilization and of the acetamidase encoded by the multiply regulated amds gene. in addition, some evidence suggests that the facb gene has a structural as well as a regulatory role in acetate metabolism. the facb gene was cloned from a cosmid library by complementation of the facb101 loss-of-function mutation. transformants receiving multiple copies of facb displayed stronger grow ... | 1989 | 2685573 |
| the highly divergent beta-tubulins of aspergillus nidulans are functionally interchangeable. | an internal 1.4-kb bst eii fragment was used to disrupt the bena gene and establish heterokaryons. the heterokaryons demonstrated that the molecular disruption of bena results in a recessive bena null mutation. conidia from a heterokaryon swell and germinate but cannot undergo nuclear division and are thus inviable. a chimeric beta-tubulin gene was constructed with the bena promoter driving the tubc structural gene. this chimeric gene construction was placed on a plasmid containing a selectable ... | 1989 | 2681229 |
| endochitinase from aspergillus nidulans implicated in the autolysis of its cell wall. | an endochitinase from centrifuged autolyzed cultures of aspergillus nidulans has been purified 100 times. the enzyme has mw 27,000, pi of 4.8 units, ph optimum around 5 ph units. it is unstable at temperature greater than 70 degrees c and does not have a cation requirement. it is inhibited by hg2+, cu2+, ca2+ and ag+ and it does not have muramidase activity. the enzyme depolymerizes chitin rapidly with production of high molecular weight polysaccharides, and then slowly degrades these with produ ... | 1989 | 2676705 |
| disparate evolution of yeasts and filamentous fungi indicated by phylogenetic analysis of glyceraldehyde-3-phosphate dehydrogenase genes. | genes encoding glyceraldehyde-3-phosphate dehydrogenase (ec 1.2.1.12) from several evolutionarily disparate organisms were used to construct a phylogenetic tree by evolutionary parsimony. the gapdh tree indicates that, in contrast to the presently accepted taxonomy of fungi, the yeasts saccharomyces cerevisiae and zygosaccharomyces rouxii evolved separately from the filamentous ascomycetes (such as aspergillus nidulans) with which these yeasts are classified. according to this tree, the saccharo ... | 1989 | 2674943 |
| characterization of an inducible expression system in aspergillus nidulans using alca and tubulin-coding genes. | plasmids have been constructed in which expression of a gene can be placed under the control of the inducible promoter of the alca gene encoding alcohol dehydrogenase i in aspergillus nidulans. simplified shuttle vectors carrying pyr4 which complements pyrg89 mutations have also been constructed. these are based on puc19 and retain alpha-peptide expression. the beta-tubulin genes, tubc and bena, have been placed under the control of alca and their expression studied. levels of expression can be ... | 1989 | 2673931 |
| cloning of the crea gene from aspergillus nidulans: a gene involved in carbon catabolite repression. | the crea gene from a. nidulans has been cloned by complementation of a non-revertable mutant allele using a genomic library and marker rescue techniques. the rescued sequence was subcloned and a 2.3 kb fragment identified which complements several crea mutant alleles. northern analyses showed that crea encodes a transcript of approximately 1.8 kb in length and that the levels of this transcript varied by up to two fold depending on the carbon source. transformants containing more than two extra ... | 1989 | 2673558 |
| transformation of seven species of filamentous fungi using the nitrate reductase gene of aspergillus nidulans. | a gene transfer system originally developed for fusarium oxysporum has been applied to seven species of filamentous fungi of agricultural and industrial importance. this transformation system relies on the selection of mutants deficient in nitrate reductase by positive screening. such mutants were recovered easily in all the fungi tested--without mutagenic treatments--through their resistance to chlorate. they were transformed by a plasmid vector (pan301) carrying the aspergillus nidulans wild-t ... | 1989 | 2673557 |
| a single, phosphate-repressible deoxyribonuclease, dnase a, secreted in aspergillus nidulans. | high levels of nuclease activities were identified in filtrates of aspergillus cultures after growth in low-but not in high-phosphate media. deoxyribonuclease activities, characterized extensively by column chromatography, showed a coincident single peak for ss- and ds-dnase which was distinct from the peak for rnase. both ss-dnase and ds-dnase are endonucleolytic and showed the highest activity in the presence of ca2+ and mn2+ (at ph 8.0). they also showed identical heat sensitivities suggestin ... | 1989 | 2673210 |
| gene function identified by interspecific transformation. | aspergillus nidulans is able to utilize 2-pyrrolidinone as a nitrogen source while two related aspergillus species, a. niger and a. terreus, cannot. mutations in the lama gene of a. nidulans prevent growth on 2-pyrrolidinone. a plasmid (plam7) has been isolated containing the a. nidulans lama gene and a divergently transcribed adjacent gene of unknown function. transformation of a. terreus with subclones of plam7 showed that both genes are essential for the utilization of a new nitrogen source, ... | 1989 | 2670678 |
| cloning of the nitrate reductase gene (niad) of aspergillus nidulans and its use for transformation of fusarium oxysporum. | an heterologous transformation system for the phytopathogenic fungus fusarium oxysporum has been developed based on the use of the aspergillus nidulans nitrate reductase gene (niad). f. oxysporum nia- mutants were easily selected by chlorate resistance. the a. nidulans niad gene was isolated from a gene library by complementation of an a. nidulans niad mutant. the cloned gene is capable of transforming f. oxysporum nia- mutants at a frequency of up to ten transformants per microgram of dna. sout ... | 1989 | 2670677 |
| a gene coding for the uric acid-xanthine permease of aspergillus nidulans: inactivational cloning, characterization, and sequence of a cis-acting mutation. | in aspergillus nidulans, integration of transforming sequences can proceed through recombination with homologous sequences or at heterologous sites in the genome. in a strain with a large deletion in the gene coding for acetamidase (amds), a plasmid carrying this gene integrates into and inactivates uapa, the putative structural gene for uric acid-xanthine permease, with a frequency of 0.3%. the integration event occurs 3' to the open reading frame of amds. a 10-nucleotide sequence which occurs ... | 1989 | 2670668 |
| characterization of the amdr-controlled lama and lamb genes of aspergillus nidulans. | four aspergillus nidulans genes are known to be under the control of the trans-acting regulatory gene amdr. we describe the isolation and initial characterization of one of these amdr-regulated genes, lama. the lam locus, however, was found to consist of two divergently transcribed genes, the lama gene, and a new gene, also under amdr control, which we have designated lamb. using recombinant dna techniques we have constructed a strain of a. nidulans lacking a functional lamb gene. experiments co ... | 1989 | 2670667 |
| isolation and characterization of the 3-phosphoglycerate kinase gene (pgk) from the filamentous fungus trichoderma reesei. | the 3-phosphoglycerate kinase gene (pgk) from trichoderma reesei was isolated by hybridization with the corresponding saccharomyces cerevisiae pgk gene. the 1,545 nt long nucleotide sequence of the cloned gene codes for a 416 amino acid protein. the coding sequence contains two introns of 219 and 75 nt, respectively, at positions identical to those corresponding genes from the other filamentous fungi aspergillus nidulans and penicillum chrysogenum. this gene codes for two mrnas of about 1.65 kb ... | 1989 | 2670282 |
| electrophoretic karyotype of aspergillus nidulans. | an electrophoretic karyotype of aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. six chromosomal bands were separated, with two of the bands migrating as doublets. using the schizosaccharomyces pombe and saccharomyces cerevisiae chromosomes as size standards, we estimate the sizes of the chromosomes to be between 2.9 and 5.0 megabase pairs (mb) with a total genome size of approximately 31 mb. four of the eight genetic linkage groups wer ... | 1989 | 2668960 |
| nucleotide sequence of the only unidentified reading frame in the aspergillus nidulans mitochondrial genome. | 1989 | 2668894 | |
| cloning and physical characterization of the l-proline catabolism gene cluster of aspergillus nidulans. | the proline catabolism gene cluster of aspergillus nidulans was cloned using a 'brute force' technique which detects clones hybridizing to restriction fragments overlapping chromosomal rearrangements. a number of deletion mutations and a translocation mutation in the cluster have been physically mapped, and an excellent correlation between the genetic and physical maps was established. transcripts have been identified and orientated for each of the four genes of the cluster. all are monocistroni ... | 1989 | 2668692 |
| the proline transport protein of aspergillus nidulans is very similar to amino acid transporters of saccharomyces cerevisiae. | in aspergillus nidulans, the gene prnb encoding the major proline transport system is one of a cluster of four genes necessary and sufficient for the utilization of proline as sole nitrogen and/or carbon source. the prn cluster has been cloned and the sequence and transcript map of the prnb gene are presented in this paper. the predicted translated sequence consists of 570 amino acids, resulting in a molecular weight of 63,028 daltons. its hydropathy profile shows 10 hydrophobic segments typical ... | 1989 | 2664423 |
| developmentally related changes in the production and expression of endo-beta-1,4-glucanases in aspergillus nidulans. | the production and electrophoretic expression of endoglucanase(s) were compared in the wild-type and three developmental mutants of aspergillus nidulans. in the wild type, the production of endoglucanase and its distribution in extracellular and intracellular fractions varied with the age of the culture and the yield was better in stable cultures (production of conidia and cleistothecia) as compared with shake cultures (vegetative hyphae only). two developmental mutants, aco-t69 and aco-40, whic ... | 1989 | 2663642 |
| chromosomal mapping and gene disruption of the adhiii gene in aspergillus nidulans. | there are at least three alcohol dehydrogenases in aspergillus nidulans. adhiii has no obvious physiological function. we describe here the cloning of the adhiii gene (alcc), its mapping on linkage group vii by "reverse genetics", and the properties of multicopy transformants tested for their ability to grow on a range of alcohols (butan-1-ol being the best substrate tested for growth). we were unable to detect any obvious alteration in phenotype of a strain carrying a disrupted copy of the adhi ... | 1989 | 2663191 |
| the genetic analysis of mitosis in aspergillus nidulans. | we describe here recent work on the molecular genetics of mitosis in the filamentous fungus aspergillus nidulans. aspergillus is one of three simple eukaryotes with powerful genetic systems that have been used to analyze mitosis. the modern molecular biological techniques available with this organism have made it possible to use mutations to identify genes and proteins that play an important role in mitosis. three aspergillus genes that affect mitosis are described. one gene, nima, is specifical ... | 1989 | 2662965 |
| nucleotide sequence of the aspergillus nidulans mitochondrial gene for subunit 5 of nadh dehydrogenase. | 1989 | 2662141 | |
| sterigmatocystin production on complex and defined substrates. | 1989 | 2662007 | |
| isolation of a sexual sporulation hormone from aspergillus nidulans. | psi factor is a substance produced by aspergillus nidulans that induces premature sexual sporulation. chromatographic analysis of psi-active extracts showed that psi activity resides in several different forms. two of the forms, psia1 and psib1, have been isolated and have been shown to have closely similar compositions. the most abundant form, psia1, reacts with alcohols in acidic solution by the addition of one entire molecule of the alcohol. this reaction, which is reversible, suggests that p ... | 1989 | 2661541 |
| molecular characterization of the aspergillus nidulans ya locus. | we investigated the molecular organization of the region of aspergillus nidulans chromosome i containing ya, a gene encoding the developmentally regulated enzyme conidial laccase. dna fragments were identified that complemented the ya2 mutation and were shown to correspond to ya by genetic mapping and gene disruption experiments. the molecular map of the region was oriented to the genetic map by testing dna fragments for their ability to complement a mutation in the tightly linked ade gene. the ... | 1989 | 2659435 |
| the mitochondrial ribosomal rna molecules of aspergillus nidulans. | the 16s and 23s mitochondrial rrnas of aspergillus nidulans have been identified by northern hybridisation and the ends of the molecules mapped onto the mitochondrial genome by s1 nuclease analysis. the results show that both the rrna molecules are longer than originally reported, forcing a reassessment of the potential secondary structures that can form in the terminal regions. in particular, structures resembling the 5.8s- and 4.5s-like domains of the bacterial large rrna can now be recognised ... | 1989 | 2656406 |
| interactions of three sequentially expressed genes control temporal and spatial specificity in aspergillus development. | aspergillus nidulans brla, abaa, and weta form a dependent pathway that regulates asexual reproductive development. the order in which these genes are expressed determines the outcome of development. expression of brla in vegetative cells leads to activation of abaa and weta, cessation of vegetative growth, cellular vacuolization, and spore formation. by contrast, expression of abaa in vegetative cells does not result in conidial differentiation but does lead to activation of brla and weta, cess ... | 1989 | 2655931 |
| different action of mms and ems in uv-sensitive strains of aspergillus nidulans. | the repair of methyl methanesulfonate (mms) and ethyl methanesulfonate (ems) damages has been investigated in the fungus aspergillus nidulans. 4 uv-sensitive mutants, namely uvsb, uvsd, uvsf and uvsh have been tested for their sensitivity and mutability to the above-mentioned agents. the results obtained show that: (1) uvsb and uvsd mutants are no more sensitive than the wild-type strain to the lethal action of ems. in contrast, they are more sensitive to mms; (2) uvsf and uvsh mutants are more ... | 1989 | 2654626 |
| beta-lactam biosynthetic genes. | 1989 | 2654524 | |
| interrelated regulation of sulphur-containing amino-acid biosynthetic enzymes and folate-metabolizing enzymes in aspergillus nidulans. | in aspergillus nidulans homocysteine can be metabolized both to cysteine and methionine. mutants impaired in the main pathway of cysteine synthesis or in the sulphate assimilation pathway show a low pool of glutathione and elevated levels of homocysteine synthase and of the homocysteine-to-cysteine pathway enzymes. on the other hand, the level of methionine synthase and other enzymes of folate metabolism is depressed in these mutants. this anticoordinated regulation provides a mechanism controll ... | 1989 | 2653822 |
| regulatory genes in aspergillus nidulans. | a major area for the study of gene regulation in lower eukaryotes has been the coordinated control of catabolic enzyme synthesis. studies of catabolic gene regulation aim to define how interactions between input signals and regulatory proteins are transmitted to the transcription machinery to bring about changes in gene expression. in the past, mutants altered in the utilization of a wide variety of substrates have been characterized in aspergillus nidulans. recently, the development of a transf ... | 1989 | 2652389 |
| a translocation associated, loss-of-function mutation in the nitrogen metabolite repression regulatory gene of aspergillus nidulans can revert intracistronically. | the arear-18 mutation is a loss-of-function mutation in area, the positive acting regulatory gene mediating nitrogen metabolite repression in aspergillus nidulans. it results from a reciprocal translocation which splits the coding region into 5' and 3' moieties. surprisingly, we have selected rare intracistronic revertants of arear-18. from crosses heterozygous for arear-18 revertant alleles, duplication-deficiency progeny containing two copies of a substantial portion of chromosome iv but lacki ... | 1989 | 2651886 |
| transformation in fungi. | transformation with exogenous deoxyribonucleic acid (dna) now appears to be possible with all fungal species, or at least all that can be grown in culture. this field of research is at present dominated by saccharomyces cerevisiae and two filamentous members of the class ascomycetes, aspergillus nidulans and neurospora crassa, with substantial contributions also from fission yeast (schizosaccharomyces pombe) and another filamentous member of the class ascomycetes, podospora anserina. however, tr ... | 1989 | 2651864 |
| identification of gamma-tubulin, a new member of the tubulin superfamily encoded by mipa gene of aspergillus nidulans. | microtubules, which are essential for mitosis and many other cytoskeletal functions, are composed primarily of alpha- and beta-tubulin. the properties of microtubules are due, in part, to proteins other than tubulins that are part of, or interact with, microtubules and the identification and characterization of such proteins is important to understanding how microtubules function. analyses of mutations at the mipa (microtubule interacting protein) locus of aspergillus nidulans have suggested tha ... | 1989 | 2649796 |
| genotoxic effects of niclosamide in aspergillus nidulans. | a 2-5-month treatment with niclosamide, a widely used drug in developing countries, has been reported to induce lymphosarcomas in toad liver and kidney. the genotoxic effects of this drug have also been evaluated in salmonella typhimurium, in somatic and germinal cells of mice and in human lymphocytes exposed in vitro and in vivo. the present study shows that niclosamide is also capable of inducing mitotic crossing-over and non-disjunction in aspergillus nidulans, which points to the wide potent ... | 1989 | 2649793 |
| delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine synthetase from aspergillus nidulans. the first enzyme in penicillin biosynthesis is a multifunctional peptide synthetase. | a multienzyme catalyzing the formation of delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine, the first free intermediate in penicillin biosynthesis, was detected in an assay measuring the formation of tripeptide from l-[u-14c]valine in the presence of l-alpha-aminoadipic acid, l-cysteine, atp, mg2+ ions, and dithioerythritol. enzyme was extracted from dry mycelium using a buffer with a high glycerol concentration and thiol protective agent to stabilize enzyme activity. in five steps the enzyme wa ... | 1989 | 2645274 |
| amplified expression of ribulose bisphosphate carboxylase/oxygenase in pbr322-transformants of anacystis nidulans. | prior research suggested that the genes for large (l) and small (s) subunits of ribulose bisphosphate carboxylase/oxygenase (rubisco) are amplified in ampicillin-resistant pbr322-transformants of anacystis nidulans 6301. we now report that chromosomal dna from either untransformed or transformed a. nidulans cells hybridizes with nick-translated [32p]-pbr322 at moderately high stringency. moreover, nick-translated [32-p]-pcs75, which is a puc9 derivative containing a psti insert with l and s subu ... | 1989 | 2644909 |
| tryptophan auxotrophic mutants in aspergillus niger: inactivation of the trpc gene by cotransformation mutagenesis. | aspergillus niger tryptophan auxotrophic mutants have been isolated after uv irradiation of conidiospores. the mutants belong to two different complementation groups, trpa and trpb, which complement each other in heterokaryons. neither of the mutations could be complemented with the cloned a. niger trpc gene. to obtain a. niger trpc mutants in a direct way, gene inactivation by cotransformation was performed. for this purpose an in-frame gene fusion between the a. niger trpc and escherichia coli ... | 1989 | 2615762 |
| physical characterization of the aldehyde-dehydrogenase-encoding gene of aspergillus niger. | to facilitate a better understanding of the regulation of alda, the gene encoding aldehyde dehydrogenase (alddh) in the ascomycete fungus, aspergillus niger, the gene has been physically characterized. the complete nucleotide (nt) sequence of the gene and its flanking regions has been determined. analysis of the gene has revealed the presence of three introns. the homologous gene in the related fungus, aspergillus nidulans, contains only two introns. the coding regions of these genes, excluding ... | 1989 | 2606357 |
| heterogeneity of 5s rna in fungal ribosomes. | neurospora crassa has at least seven types of 5s rna genes (alpha, beta, gamma, epsilon, delta, zeta, and eta) with different coding regions. a high resolution gel electrophoresis system was developed to separate minor 5s rna's from the major 5s rna (alpha). a study of several neurospora crassa strains, four other species in the genus neurospora, members of two closely related genera, and three distantly related genera demonstrated that 5s rna heterogeneity is common among fungi. in addition, di ... | 1985 | 2579431 |
| ammonia assimilation by aspergillus nidulans: [15n]ammonia study. | 15n kinetic labelling studies were done on liquid cultures of wild-type aspergillus nidulans. the labelling pattern of major amino acids under 'steady state' conditions suggests that glutamate and glutamine-amide are the early products of ammonia assimilation in a. nidulans. in the presence of phosphinothricin, an inhibitor or glutamine synthetase, 15n labelling of glutamate, alanine and aspartate was maintained whereas the labelling of glutamine was low. this pattern of labelling is consistent ... | 1989 | 2574737 |
| isolation of the faca (acetyl-coenzyme a synthetase) and acue (malate synthase) genes of aspergillus nidulans. | acetate inducible genes of aspergillus nidulans were cloned via differential hybridization to cdna probes. using transformation of mutant strains the genes were identified as faca (acetyl-coenzyme a synthetase) and acue (malate synthase). the levels of rna encoded by these genes were shown to be acetate inducible and subject to carbon catabolite repression. induction is abolished in a facb mutant and carbon catabolite repression is relieved in a crea mutant. | 1989 | 2571070 |
| cloning of a new bidirectionally selectable marker for aspergillus strains. | mutants that lack adenosine triphosphate sulfurylase (atpsase; ec 2.7.7.4) are unable to use sulfate as sole source of sulfur and are also resistant to selenate. these mutants, denoted sc-, are readily obtained from any strain of aspergillus niger or aspergillus nidulans by the strong selection for selenate resistance. we have cloned the gene encoding atpsase from a. nidulans by complementation of an sc mutant strain of a. nidulans with a gene library and show that plasmids containing this gene ... | 1989 | 2558969 |
| invasive aspergillus infection in chronic granulomatous disease: treatment with itraconazole. | 1989 | 2555469 | |
| nucleotide sequence and regulation of expression of the aspergillus nidulans gdha gene encoding nadp dependent glutamate dehydrogenase. | the nucleotide sequence of the aspergillus nidulans gdha gene encoding nadp linked glutamate dehydrogenase has been determined and northern blot analysis used to study the regulation of expression of this gene. the gdha gene is 1485 nucleotides long and, by comparison with the corresponding neurospora crassa am gene, has two putative introns of 53 nucleotides and a protein encoding region of 1380 nucleotides that codes for an inferred protein of 49.63 kda which shows regions of homology with glu ... | 1989 | 2550758 |
| the bimg gene of aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1. | in aspergillus nidulans, the temperature-sensitive, recessive cell cycle mutation bimg11 causes an elevated mitotic index at restrictive temperature and an inability to complete the anaphase separation of daughter nuclei. we have shown that this mutation has an abnormally high content of nuclear phosphoproteins and that the wild-type gene encodes a type 1 protein phosphatase. we conclude that dephosphorylation of a key protein(s) is required to complete mitosis. | 1989 | 2544297 |
| protein encoded by the third intron of cytochrome b gene in saccharomyces cerevisiae is an mrna maturase. analysis of mitochondrial mutants, rna transcripts proteins and evolutionary relationships. | we have established the nucleotide sequence of the wild-type and that of a trans-acting mutant located in the third (bi3) intron of the saccharomyces cerevisiae mitochondrial cytochrome b gene. the intron, 1691 base-pairs long, has an open reading frame 1045 base-pairs long, in phase with the preceding exon and the mutation replaces the evolutionarily conserved gly codon of the second consensus motif by an asp codon and blocks the formation of mature cytochrome b mrna. splicing intermediates of ... | 1989 | 2538624 |
| regulation of the aspergillus nidulans pectate lyase gene (pela). | aspergillus nidulans pectate lyase was purified from culture filtrates. the enzyme catalyzed a random eliminative cleavage reaction, had an apparent molecular weight of 40,000, and a pl of 4.2. pectate lyase antisera were produced and used to identify pectate lyase clones in a cdna expression library. thirteen of 14 clones identified immunologically cross-hybridized. the identity of the single-copy pectate lyase gene, which we designated pela, was confirmed in two ways. first, several cdna clone ... | 1989 | 2535502 |
| production of cell wall-degrading enzymes by aspergillus nidulans: a model system for fungal pathogenesis of plants. | the cell wall-degrading enzymes polygalacturonase and pectate lyase have been suggested to be crucial for penetration and colonization of plant tissues by some fungal pathogens. we have found that aspergillus nidulans (= emericella nidulans), a saprophytic ascomycete, produces levels of these enzymes equal to those produced by soft-rotting erwinia species. induction of polygacturonase and pectate lyase in a. nidulans requires substrate and is completely repressed by glucose. surprisingly, inocul ... | 1989 | 2535501 |
| processing of mitochondrial rna in aspergillus nidulans. | genes for cytochrome oxidase subunit i (oxia), atpase subunit 9, nadh dehydrogenase subunit 3 (ndhc) and cytochrome oxidase subunit ii (oxib) are located within a 7.2 kb (1 kb = 10(3) bases or base-pairs) segment of the aspergillus nidulans mitochondrial genome. northern hybridization shows that abundant rna molecules of 4.0, 2.5 and 1.5 kb, each containing copies of two or more genes, are transcribed from this region. the 4.0 kb molecule, which contains copies of each of the four genes but lack ... | 1989 | 2530353 |
| genetic engineering of filamentous fungi. | filamentous fungi are important in medicine, industry, agriculture, and basic biological research. for example, some fungal species are pathogenic to humans, whereas others produce beta-lactam antibiotics (penicillin and cephalosporin). industrial strains produce large amounts of enzymes, such as glucoamylase and proteases, and low molecular weight compounds, such as citric acid. the largest and most economically important group of plant pathogens are fungi. several fungal species have biologica ... | 1989 | 2525275 |
| purification and characterisation of nad-glutamate dehydrogenase from aspergillus nidulans. | nad-glutamate dehydrogenase has been purified from mycelia of a. nidulans. the enzyme comprises subunits of 110 kda. it is located in the cytosol. it is completely denatured by 1.0 m guanidine hydrochloride, and is not renatured by subsequent dilution. isophthalate is a strong competitive inhibitor and the enzyme is also inhibited by thiol reagents. the properties of the enzyme were compared to those from other fungi in terms of size, sensitivity to inhibitors, intracellular distribution and mod ... | 1989 | 2524418 |
| cobalt-induced inhibition of growth in cyanobacteria anabaena doliolum and anacystis nidulans: interaction with sulphur containing amino acids. | cobalt, above 1 microm concentration was growth inhibitory for both a. doliolum and a. nidulans. its toxicity was mitigated by sulphur containing amino acids (cystine and cysteine), however, methionine could not mitigate the cobalt toxicity at all. | 1989 | 2517422 |
| expression of an anacystis nidulans photolyase gene in escherichia coli; functional complementation and modified action spectrum of photoreactivation. | the anacystis nidulans photolyase gene inserted in an expression vector plasmid was introduced into escherichia coli cells and the production of anacystis photolyase protein was confirmed by reaction with antibodies raised against photolyase purified from a. nidulans cells. the anacystis photolyase functioned in photoreactivation repair defective e. coli cells. the e. coli transformants exhibited an action spectrum with a maximum around 380 nm similar to that of e. coli photolyase in contrast wi ... | 1989 | 2516329 |
| high-performance liquid chromatographic determination of delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine in complex media by precolumn derivatisation with dansylaziridine. | a novel method is described for the trace level quantitation of the tripeptide delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine (acv) in complex fermentation media, using a high-performance liquid chromatographic, pre-column derivatisation technique. the procedure is based upon the reaction of the acv monomer with 5-dimethylaminonaphthalene-1-sulphonylaziridine (dansylaziridine) and produces a highly fluorescent product. reaction conditions between the reagent and tripeptide were investigated an ... | 1989 | 2512319 |
| [properties of 2,5-diamino-4-oxy-6-ribosylaminopyrimidine-5'- phosphate reductase, a enzyme of the second stage of flavinogenesis in pichia guilliermondii yeasts]. | 2,5-diamino-4-oxy-6-ribosylaminopyrimidine-5'-phosphate reductase has been isolated from cells of pichia guilliermondii and subjected to 20-fold purification by treating extracts with streptomycin sulphate, frationating proteins (nh4)2so4 at 45-75% of saturation and chromatography on blue sepharose cl-6b. the use of gel filtration through sephadex g-150 and chromatography on deae-cellulose proved to be less effective for the enzyme purification. it has been established that it is 2,5-diamino-4-o ... | 1989 | 2511652 |
| aspergillus and mouse share a new class of 'zinc finger' protein. | 1989 | 2511649 | |
| duplication of the phycocyanin operon in the unicellular cyanobacterium anacystis nidulans r2. | two phycocyanin (pc) operons, each containing alpha- and beta-subunit genes, have been isolated from the unicellular cyanobacterium anacystis nidulans r2. using oligodeoxyribonucleotide probes for the pc-coding regions, three psti fragments were obtained and shown to contain the two operons, which are 2.7 kb apart, with a proposed gene order of 5'-(beta i-alpha i)-(beta ii-alpha ii)-3'. the nucleotide sequences of both alpha-subunit genes are identical, as are the beta-sequences and the 51-bp in ... | 1989 | 2511077 |
| expression of the trpc gene from penicillium chrysogenum in aspergillus nidulans. | the heterologous expression in aspergillus nidulans of a gene involved in tryptophan biosynthesis from penicillium chrysogenum is described. with the chimeric plasmid ppc-31, which carries the cloned trpc gene, approximately 10-40 "stable" transformants per microgram of dna were obtained, with selection for complementation of the mutant allele. this frequency was increased 10-fold by the insertion of the ans1 fragment into the transformation vector. southern hybridization analysis revealed that ... | 1989 | 2508698 |
| cloning and molecular characterisation of the amdr controlled gata gene of aspergillus nidulans. | the gamma-amino-n-butyrate transaminase gene (gata) of aspergillus nidulans is one of several genes under positive control by the regulatory gene amdr (also called inta). the gata gene has been cloned from a cosmid library by complementation of a gata mutation. the sequence of a 2.6 kb genomic fragment containing gata has been determined. an open reading frame of 1497 bp within this sequence is interrupted by three putative introns and predicts a protein of 55 kda. northern analysis confirms con ... | 1989 | 2505051 |
| expression of the escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi. | a chimaeric beta-glucuronidase (gus) gene has been created by ligating the aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the e. coli uida gene. co-transformation of this vector into a. nidulans, a. niger and the tomato pathogen fulvia fulva (syn. cladosporium fulvum (cooke] resulted in the expression of beta-glucuronidase. gus activity was detected by growth on agar media containing x-gluc and by enzyme assays of mycelial extracts. expression of ... | 1989 | 2504501 |
| development of an expression system in aspergillus nidulans. | 1989 | 2502450 | |
| cloning and characterization of an anacystis nidulans r2 superoxide dismutase gene. | the e. coli iron superoxide dismutase gene (sodb) was utilized as a heterologous probe to isolate a superoxide dismutase (sod) gene from anacystis nidulans r2. nucleotide sequence analysis revealed a 603 bp open reading frame with deduced amino acid sequence similar to other sod genes and to cyanobacterial superoxide dismutase amino-terminal sequences. assuming proteolytic cleavage of the initial methionine residue, the molecular mass of the mature a. nidulans r2 sodb polypeptide is 22,000 dalto ... | 1989 | 2501651 |
| cloning, sequence analysis and transcriptional study of the isopenicillin n synthase of penicillium chrysogenum as-p-78. | a gene (ips) encoding the isopenicillin n synthase of penicillium chrysogenum as-p-78 was cloned in a 3.9 kb sali fragment using a probe corresponding to the amino-terminal end of the enzyme. the sali fragment was trimmed down to a 1.3 kb ncoi-bglii fragment that contained an open reading frame of 996 nucleotides encoding a polypeptide of 331 amino acids with an mr of 38012 dalton. the predicted polypeptide encoded by the ips gene of strain as-p-78 contains a tyrosine at position 195, whereas th ... | 1989 | 2499766 |
| genes for the ribosomal proteins s12 and s7 and elongation factors ef-g and ef-tu of the cyanobacterium, anacystis nidulans: structural homology between 16s rrna and s7 mrna. | a 6.5 kb region from the genome of the cyanobacterium, anacystis nidulans 6301 was cloned using the tobacco chloroplast gene for ribosomal protein s12 as a probe. sequence analysis revealed the presence of genes for ribosomal proteins s12 and s7 and elongation factors ef-g and ef-tu in this dna region. the arrangement is rps12 (124 codons) - 167 bp spacer - rps7 (156 codons) - 77 bp spacer - fus (694 codons) - 26 bp spacer - tufa (409 codons), which is similar to that of the escherichia coli str ... | 1989 | 2499762 |
| dna sequence and secondary structures of the large subunit rrna coding regions and its two class i introns of mitochondrial dna from podospora anserina. | dna sequence analysis has shown that the gene coding for the mitochondrial (mt) large subunit ribosomal rna (rrna) from podospora anserina is interrupted by two class i introns. the coding region for the large subunit rrna itself is 3715 bp and the two introns are 1544 (r1) and 2404 (r2) bp in length. secondary structure models for the large subunit rrna were constructed and compared with the equivalent structure from escherichia coli 23s rrna. the two structures were remarkably similar despite ... | 1989 | 2494353 |
| thioredoxin is essential for photosynthetic growth. the thioredoxin m gene of anacystis nidulans. | we have taken advantage of the transformation properties of the cyanobacterium anacystis nidulans r2 to investigate the importance of thioredoxin for photosynthetic growth. the gene encoding thioredoxin m, designated trxm, was cloned from a. nidulans using a synthetic oligonucleotide probe. based on the nucleotide sequence, thioredoxin m of a. nidulans is composed of 107 amino acids and shares 84, 48, and 48% sequence identity with thioredoxins from anabaena, spinach, and escherichia coli, respe ... | 1989 | 2492995 |
| relationship between a 47-kda cytoplasmic membrane polypeptide and nitrate transport in anacystis nidulans. | the polypeptide composition of cytoplasmic membranes of the cyanobacterium anacystis nidulans changes in response to variations in the nitrogen source available to the cells, differing specifically in the amount of a polypeptide of 47-kda molecular mass. synthesis of the polypeptide and expression of nitrate transport activity are repressed by ammonium. transfer of ammonium-grown cells to a medium containing nitrate as the sole nitrogen source results in parallel development of the 47-kda polype ... | 1989 | 2492194 |
| enhancement of aspergillus nidulans transformation by the ans1 sequence. | we have shown that the aspergillus nidulans ans1 sequence enhances the efficiency of transformation when introduced into vectors containing argb or trpc genes. increased efficiency of transformation is also observed when ans1 is present on a second cotransforming plasmid. in an attempt to explains the ans1 transactivity we have performed analysis of some cotransformants. | 1989 | 2484740 |
| a large cluster of highly expressed genes is dispensable for growth and development in aspergillus nidulans. | we investigated the functions of the highly expressed, sporulation-specific spoc1 genes of aspergillus nidulans by deleting the entire 38-kb spoc1 gene cluster. the resultant mutant strain did not differ from the wild type in (1) growth rate, (2) morphology of specialized reproductive structures formed during completion of the asexual or sexual life cycles, (3) sporulation efficiency, (4) spore viability or (5) spore resistance to environmental stress. thus, deletion of the spoc1 gene cluster, r ... | 1989 | 2471671 |
| developmental characterization and chromosomal mapping of the 5-azacytidine-sensitive fluf locus of aspergillus nidulans. | in aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-ac) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (m. tamame, f. antequera, j. r. villanueva, and t. santos, mol. cell. biol. 3:2287-2297, 1983). this new 5-ac-altered locus, designated here fluf1, was mapped as the closest marker to the centromere that has been i ... | 1988 | 2463470 |
| tallysomycin-induced mitotic aneuploidy and point mutations in aspergillus nidulans. | tallysomycin is an antibiotic compound structurally related to bleomycin, and like bleomycins and phleomycins also shows antitumour activity. we have investigated the genetic activity of tallysomycin in the ascomycete aspergillus nidulans for malsegregation of chromosomes at mitosis and for point mutations. we found that the antibiotic at very low concentrations from 0.025 to 0.2 micrograms/ml had an inhibitory effect on colonial growth of up to 50% and it increased the number of mitotic malsegr ... | 1986 | 2457783 |
| distinction of cnxh cofactor gene-specified protomers with monoclonal antibodies to aspergillus nitrate reductase. | the nitrate reductase (nadph) (ec 1.6.6.3) from aspergillus nidulans is influenced directly by mutations in the structural gene (niad) for the major subunit of the enzyme and indirectly by mutation in any of several molybdenum cofactor loci (cnx). the cnxe-14 and the cnxh-3 mutants have been noted to contain the enzyme in two distinct forms following induction with nitrate. with the cnxh-3 as a prototype cnxh mutant, 10 other cnxh were found to be devoid of the assembled (dimeric) form of the en ... | 1988 | 2454152 |
| repair of alkylation damage in the fungus aspergillus nidulans. | the repair of alkylation damage in aspergillus nidulans was investigated. we have assayed soluble protein fractions for enzymes known to be involved in the repair of this type of damage in dna. the presence of a glycosylase activity that can remove 3-methyladenine from dna was demonstrated, as well as a dna methyltransferase activity that appears to act against o6-methylguanine. in addition to this approach, a series of mutants were isolated which display increased sensitivity to alkylating agen ... | 1988 | 2452348 |
| heat shock phenomena in aspergillus nidulans. ii. combined effect of heat and bleomycin to heat shock protein synthesis, survival rate and induction of mutations. | the combined action of hyperthermia and bleomycin on aspergillus nidulans was studied at three different levels: mycelial protein synthesis, spore viability and induction of mutations. it was found that bleomycin treatment of preincubated mycelia during the heat shock enhances the incorporation of 35s-methionine into heat shock bands. furthermore, simultaneous treatment with hyperthermia (43 degrees c) and bleomycin results in greater cytotoxic activity in spores and in a higher induction rate o ... | 1987 | 2452026 |
| the mitochondrial genome of the fission yeast, schizosaccharomyces pombe. sequence of the large-subunit ribosomal rna gene, comparison of potential secondary structure in fungal mitochondrial large-subunit rrnas and evolutionary considerations. | the dna sequence of the mitochondrial large subunit (lsu) rrna gene of schizosaccharomyces pombe has been determined. in the direction of transcription, this gene is located between the gene coding for subunit ii of cytochrome oxidase and a cluster of three trna genes. both the 5' and 3' ends of the lsu rrna have been mapped precisely: whereas the 5' end can be assigned unambiguously to a single nucleotide position, multiple 3' ends occur within a run of eight u residues. based on these results, ... | 1987 | 2446871 |
| transformation of aspergillus nidulans by the argb gene. | aspergillus nidulans argb mutant was transformed with the plasmid dna containing the argb gene. analysis of transformants revealed that transformation was due to integration of either argb gene alone or the whole plasmid dna into the a. nidulans genome. in 5 out of 23 transformants studied, integration took place in the locus different than the original argb locus. the amplification of integrated sequences was often observed. integrated dna was found to be mitotically stable, while the meiotic s ... | 1987 | 2442970 |
| 5s rrna genes from aspergillus nidulans are not transcribed in saccharomyces cerevisiae. | several different 5s rrna genes from aspergillus nidulans cloned in an escherichia coli--saccharomyces cerevisiae shuttle vector were introduced into s. cerevisiae cells by transformation. the a. nidulans 5s rrna genes were not transcribed in s. cerevisiae. | 1986 | 2436448 |
| probing fungal mitochondrial evolution with trna. | sequence data are now available for almost the entire complement of mitochondrial rrnas from five fungi: schizosaccharomyces pombe, saccharomyces cerevisiae, toropulis glabrata, aspergillus nidulans and neurospora crassa. analysis of these data show that the five mitochondria can be related to a common ancestor. the unusually high similarity between some s. pombe mt trnas may be due to a process similar to gene conversion. using the number of differences between trna pairs as a measure of the ev ... | 1985 | 2417640 |
| selective overexpression of the qute gene encoding catabolic 3-dehydroquinase in multicopy transformants of aspergillus nidulans. | the three enzymes necessary to catabolize quinate to protocatechuate are inducible by quinic acid, and transcription of their corresponding genes is controlled by the action of a positively acting activator gene and a negatively acting repressor gene. transformed strains of aspergillus nidulans containing multiple copies of the activator gene (quta) but single copies of the other qut genes retain normal regulation of the gene cluster and do not show any overexpression of the three quinic acid ca ... | 1990 | 2405841 |
| the aspergillus nidulans npea locus consists of three contiguous genes required for penicillin biosynthesis. | clones of aspergillus nidulans genomic dna spanning 20 kb have been isolated and shown by a combination of classical and molecular genetic means to represent the npea locus, previously found to be one of four loci (npea, npeb, npec and nped) involved in the synthesis of penicillin. as well as containing the gene encoding the second enzyme for penicillin biosynthesis, namely isopenicillin n synthetase (ipns) (designated ipna), our results show that these clones (psta200, psta201 and psta207) cont ... | 1990 | 2403928 |
| homologous transformation of cephalosporium acremonium with the nitrate reductase-encoding gene (niad). | we report the development of a homologous transformation system for cephalosporium acremonium using the niad gene of the nitrate assimilation (na) pathway. mutants in the na pathway were selected on the basis of chlorate resistance by conventional means. screening procedures were developed to differentiate between nitrate reductase apoprotein structural gene mutants (niad) and molybdenum cofactor gene mutants (cnx) as wt c. acremonium, unlike most filamentous fungi, fails to grow on minimal medi ... | 1990 | 2401400 |
| cloning and analysis of beta-tubulin gene from a protoctist. | we have isolated and characterized by restriction endonuclease mapping, transcription pattern, and dna sequencing a beta-tubulin gene from the coenocytic freshwater protoctist, achlya klebsiana. the gene is intronless and has a single open reading frame that encodes a 444-amino acid residue polypeptide of mr 49,856. the protein shows a high degree of homology to other beta-tubulins, 85% identity to human beta-tubulin and 89% identity to beta-tubulin of the sporozoan (also a protoctist) plasmodiu ... | 1990 | 2394720 |
| survey of the mycoflora and mycotoxins of cotton seeds and cotton seed products in egypt. | thirty-nine species and 16 fungal genera were isolated from egyptian cotton seeds, cotton seed meal and cotton seed cake on 1% glucose-czapek's agar medium incubated at 28 degrees c. aspergillus was the most frequent genus and it emerged in 87-100% of the samples contributing 70-98% of total fungi in the three substrates tested. the most common species were a. niger, a. flavus, a. fumigatus, a. terreus and rhizopus stolonifer; a. niger, a. fumigatus and penicillium corylophilum; and a. niger, a. ... | 1990 | 2388680 |
| high-efficiency transformation system for the biocontrol agents, trichoderma spp. | we have developed an efficient transformation system based on the use of polyethylene glycol and cacl2 for the biocontrol agents, trichoderma spp. transformation was obtained with the plasmid pan7-1, carrying a bacterial hygromycin-resistance gene as a selectable marker, under the control of aspergillus nidulans heterologous expression signals. the system described here yielded 200-800 transformants per microgram of dna. transformants contained several copies of the plasmid integrated into their ... | 1990 | 2388561 |
| localization of pyruvate carboxylase in organic acid-producing aspergillus strains. | the localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different aspergillus species (14 strains). in some species (a. aculeatus, a. flavus, a. foetidus, a. nidulans, a. ochraceus, and a. sojae), the pyruvate carboxylase activity could be detected only in the cytosolic fraction of the cells. pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of aspergillus wentii. in aspergillus oryzae and in five strains of aspergillus niger, ... | 1990 | 2383004 |