Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| convergent evolution and orphan genes in the fur4p-like family and characterization of a general nucleoside transporter in aspergillus nidulans. | the function of seven paralogues phylogenetically related to the saccharomyces cerevisiae fur4p together with a number of functionally related transporters present in aspergillus nidulans has been investigated. after deletion of the cognate genes we checked the incorporation of radiolabelled substrates, utilization of nitrogen sources, resistance to toxic analogues and supplementation of auxotrophies. fura and furd encode allantoin and uracil transporters respectively. no function was found for ... | 2009 | 19460095 |
| the vea gene is necessary for the negative regulation of the vea expression in aspergillus nidulans. | the vea gene is one of the key genes in regulating sexual development of aspergillus nidulans. during the study on the vea gene, it was observed that the vea expression level is slightly higher in a vea1 mutant than in a wild type at 37 degrees c, suggesting that the wild type vea gene is necessary for the negative regulation of the vea expression. in the vea1 mutant, the vea expression was higher than in a wild type grown at 42 degrees c but equal at 30 degrees c. furthermore, in a vea deletion ... | 2009 | 19479257 |
| expression of a gfp gene in penicillium nordicum under control of the promoter of the ochratoxin a polyketide synthase gene. | a gfp reporter gene strain of penicillium nordicum was constructed in order to be able to study the influence of environmental parameters on ochratoxin a biosynthesis. to introduce the gfp gene an agrobacterium tumefaciens mediated transformation system (atmt) for p. nordicum was established resulting in a transformation efficiency of about 60 transformants per microg of dna. the selection principle was based on the hygromycin b resistance gene located on the ti-dna fragment of the binary vector ... | 2009 | 19525025 |
| modelling and mutational evidence identify the substrate binding site and functional elements in apc amino acid transporters. | the amino acid-polyamine-organocation (apc) superfamily is the main family of amino acid transporters found in all domains of life and one of the largest families of secondary transporters. here, using a sensitive homology threading approach and modelling we show that the predicted structure of apc members is extremely similar to the crystal structures of several prokaryotic transporters belonging to evolutionary distinct protein families with different substrate specificities. all of these prot ... | 2009 | 19670073 |
| functional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from penicillium paxilli. | the filamentous fungus penicillium paxilli contains two distinct geranylgeranyl diphosphate (ggpp) synthases, ggsa and ggsb (paxg). paxg and its homologues in neotyphodium lolii and fusarium fujikuroi are associated with diterpene secondary metabolite gene clusters. the genomes of other filamentous fungi including aspergillus fumigatus, aspergillus nidulans, aspergillus niger, aspergillus oryzae and fusarium graminearum also contain two or more copies of ggpp synthase genes, although the diterpe ... | 2009 | 19529962 |
| characterization of the aspergillus ochraceoroseus aflatoxin/sterigmatocystin biosynthetic gene cluster. | production of carcinogenic aflatoxins has been reported from members of aspergillus section flavi, aspergillus section nidulantes and a newly proposed aspergillus section ochraceorosei that consists of aspergillus ochraceoroseus and a. rambellii. unlike members of section flavi, a. ochraceoroseus and a. rambellii have been shown to accumulate both aflatoxin (af) and the aflatoxin precursor sterigmatocystin (st). alhough morphologically distinct from a. nidulans, molecular characterization of a. ... | 2009 | 19537208 |
| patterns of susceptibility of aspergillus isolates recovered from patients enrolled in the transplant-associated infection surveillance network. | we analyzed antifungal susceptibilities of 274 clinical aspergillus isolates from transplant recipients with proven or probable invasive aspergillosis collected as part of the transplant-associated infection surveillance network (transnet) and examined the relationship between mic and mortality at 6 or 12 weeks. antifungal susceptibility testing was performed by the clinical and laboratory standards institute (clsi) m38-a2 broth dilution method for amphotericin b (amb), itraconazole (itr), voric ... | 2009 | 19692558 |
| molecular characterization of a fungal gene paralogue of the penicillin pende gene of penicillium chrysogenum. | penicillium chrysogenum converts isopenicillin n (ipn) into hydrophobic penicillins by means of the peroxisomal ipn acyltransferase (iat), which is encoded by the pende gene. in silico analysis of the p. chrysogenum genome revealed the presence of a gene, pc13g09140, initially described as paralogue of the iat-encoding pende gene. we have termed this gene ial because it encodes a protein with high similarity to iat (ial for iat-like). we have conducted an investigation to characterize the ial ge ... | 2009 | 19470155 |
| the cell end marker protein teac is involved in growth directionality and septation in aspergillus nidulans. | polarized growth in filamentous fungi depends on the correct spatial organization of the microtubule (mt) and actin cytoskeleton. in schizosaccharomyces pombe it was shown that the mt cytoskeleton is required for the delivery of so-called cell end marker proteins, e.g., tea1 and tea4, to the cell poles. subsequently, these markers recruit several proteins required for polarized growth, e.g., a formin, which catalyzes actin cable formation. the latest results suggest that this machinery is conser ... | 2009 | 19429780 |
| restoration of net formation by gene therapy in cgd controls aspergillosis. | chronic granulomatous disease (cgd) patients have impaired nicotinamide adenine dinucleotide phosphate (nadph) oxidase function, resulting in poor antimicrobial activity of neutrophils, including the inability to generate neutrophil extracellular traps (nets). invasive aspergillosis is the leading cause of death in patients with cgd; it is unclear how neutrophils control aspergillus species in healthy persons. the aim of this study was to determine whether gene therapy restores net formation in ... | 2009 | 19541821 |
| effect of ozone on spore germination, spore production and biomass production in two aspergillus species. | the ability of ozone gas to reduce food spoilage is relatively well documented, but the developmental effects of the gas on food spoilage fungi are not well known. in this study two model aspergilli, aspergillus nidulans and aspergillus ochraceus were used to study the effects of ozone on spore germination, sporulation and biomass production. these effects were examined under three levels of ozone; two high level ozone exposures (200 and 300 micromol mol(-1)) and one low level exposure (0.2 micr ... | 2009 | 19533409 |
| kapi, a non-essential member of the pse1p/imp5 karyopherin family, controls colonial and asexual development in aspergillus nidulans. | asexual development in the filamentous fungus aspergillus nidulans is governed by the timely expression and cellular localization of multiple transcription factors. hence, factors mediating import and export across the nuclear pore complexes (karyopherins) are expected to play a key role in coordinating the developmental programme. here we characterize kapi, a putative homologue of the saccharomyces cerevisiae kap121/pse1p karyopherin. kapi is a non-essential importin-beta-like protein located i ... | 2009 | 19729403 |
| negative roles of a novel nitrogen metabolite repression-related gene, tar1, in laccase production and nitrate utilization by the basidiomycete cryptococcus neoformans. | the multicopper oxidase laccase is widespread in fungi and has great industrial importance. one puzzle regarding laccase production in the basidiomycetous yeast cryptococcus neoformans is that it is inhibited by high temperature (e.g., 37 degrees c). in this paper, we report identification of a nitrogen metabolite repression-related gene, tar1, which is responsible for laccase repression. disruption of tar1 results in a significant increase in the level of lac1 mrna at 37 degrees c. the putative ... | 2009 | 19734333 |
| first case of extensive spinal cord infection with aspergillus nidulans in a child with chronic granulomatous disease. | chronic granulomatous disease (cgd) is characterized by a defect in phagocytic cells that lead to recurrent bacterial and fungal infections. the etiology of most common fungal infections in cgd are aspergillus species. aspergillus nidulans is one of several species of aspergillus with low pathogenicity. however, it was reported to cause fatal invasive aspergillosis in patients with cgd. here we report the first cured invasive aspergillus nidulans infection with extensive involvement of the spina ... | 2009 | 19759498 |
| prophylaxis against aspergillus is not perfect: problems and perils in stem cell transplantation. | availability of newer, well tolerated anti-aspergillus drugs at a time of rising prevalence of invasive aspergillosis has sparked enthusiasm for chemoprophylaxis against aspergillus in allogeneic stem cell recipients. such a drug-oriented approach however is associated with problems. in the azole class, itraconazole has been shown to be effective, but hepatotoxicity and gastrointestinal intolerability have made the drug less attractive. posaconazole, approved for prophylaxis in allogeneic stem c ... | 2009 | 18663660 |
| mapping the interaction sites of aspergillus nidulans phytochrome fpha with the global regulator vea and the white collar protein lreb. | aspergillus nidulans senses red and blue-light and employs a phytochrome and a neurospora crassa white collar (wc) homologous system for light perception and transmits this information into developmental decisions. under light conditions it undergoes asexual development and in the dark it develops sexually. the phytochrome fpha consists of a light sensory domain and a signal output domain, consisting of a histidine kinase and a response regulator domain. previously it was shown that the phytochr ... | 2009 | 18936976 |
| functional diversity of chitin synthases of aspergillus nidulans in hyphal growth, conidiophore development and septum formation. | fungal chitin synthases are classified into seven classes. recent advances in genome sequence analyses have revealed that there are more than seven chitin synthase-encoding genes in the genome of filamentous ascomycete fungi. to clarify the functional differences among these genes in the morphogenesis and hyphal tip growth, we have cloned six chitin synthase-encoding genes from aspergillus nidulans and analyzed their functions. the results obtained suggest that chitin synthases are structurally ... | 2009 | 18651309 |
| molecular detection and identification of aspergillus spp. from clinical samples using real-time pcr. | the definite and rapid diagnosis of invasive aspergillosis is necessary because of the high mortality caused. the objective of this study was to evaluate a real-time pcr assay to detect aspergillus spp. in clinical samples, based on the light cycler technology. specificity was assessed by using dna extracted from pathogenic and non-pathogenic bacteria/fungi from spanish collection including: two aspergillus flavus, four aspergillus fumigatus, two aspergillus nidulans, two aspergillus niger and t ... | 2009 | 18643921 |
| effect of culture temperature on the heterologous expression of pleurotus eryngii versatile peroxidase in aspergillus hosts. | production of recombinant versatile peroxidase in aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. to further this purpose, the cdna encoding a versatile peroxidase of pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alca) promoter of aspergillus nidulans. a dependence of recombinant peroxidase production on cultivation temperature was found. lowering the culture temperature from 28 to 19 degrees c enhanced the lev ... | 2009 | 18481101 |
| proteins involved in microbody biogenesis and degradation in aspergillus nidulans. | fungal microbodies (peroxisomes) are inducible organelles that proliferate in response to nutritional cues. proteins involved in peroxisome biogenesis/proliferation are designated peroxins and are encoded by pex genes. an autophagy-related process, termed pexophagy, is responsible for the selective removal of peroxisomes from the cell. several genes involved in pexophagy are also required for autophagy and are collectively known as atg genes. we have re-analysed the aspergillus nidulans genome f ... | 2009 | 18694841 |
| recombinant bacterial hemoglobin alters metabolism of aspergillus niger. | the filamentous fungus aspergillus niger is used extensively for the production of enzymes and organic acids. a major problem in industrial fermentations with this fungus is to ensure sufficient supply of oxygen required for respiratory metabolism of the fungus. in case of oxygen limitation, the fungus will produce various by-products like organic acids and polyols. in order to circumvent this problem we here study the effects of the expression of a bacterial hemoglobin protein on the metabolism ... | 2009 | 18694843 |
| genome-wide analysis of microsatellite sequence in seven filamentous fungi. | abundance of microsatellites with repeated unit lengths of 1-6 base pairs in seven fungi: aspergillus nidulans, coprinus cinereus, cryptococcus neoformans (serotype a), fusarium graminearum, magnaporthe grisea, neurospora crassa and ustilago maydis were investigated on genomic scale. the results showed that each species has its specific profile for different types and different motifs of ssr loci. ascomycetes fungi m. grisea, n. crassa and basidiomycete fungus u. maydis adopt much more microsate ... | 2009 | 20640828 |
| molecular characterization of the niad and pyrg genes from penicillium camemberti, and their use as transformation markers. | genetic manipulation of the filamentous fungus penicillium camemberti has been limited by a lack of suitable genetics tools for this fungus. in particular, there is no available homologous transformation system. in this study, the nitrate reductase (niad) and orotidine-5'-monophosphate decarboxylase (pyrg) genes from penicillium camemberti were characterized, and their suitability as metabolic molecular markers for transformation was evaluated. the genes were amplified using pcr-related techniqu ... | 2009 | 19562269 |
| dynein light intermediate chain in aspergillus nidulans is essential for the interaction between heavy and intermediate chains. | cytoplasmic dynein is a complex containing heavy chains (hcs), intermediate chains (ics), light intermediate chains (lics), and light chains (lcs). the hcs are responsible for motor activity. the ics at the tail region of the motor interact with dynactin, which is essential for dynein function. however, functions of other subunits and how they contribute to the assembly of the core complex are not clearly defined. here, we analyzed in the filamentous fungus aspergillus nidulans functions of the ... | 2009 | 19837669 |
| the properties of adaptive walks in evolving populations of fungus. | the rarity of beneficial mutations has frustrated efforts to develop a quantitative theory of adaptation. recent models of adaptive walks, the sequential substitution of beneficial mutations by selection, make two compelling predictions: adaptive walks should be short, and fitness increases should become exponentially smaller as successive mutations fix. we estimated the number and fitness effects of beneficial mutations in each of 118 replicate lineages of aspergillus nidulans evolving for appr ... | 2009 | 19956798 |
| bicarbonate gradients modulate growth and colony morphology in aspergillus nidulans. | fungal colonies expand through apically extending peripheral hyphae. these long and relatively unbranched leaders leave behind cells that show a higher degree of branching and lower growth rates. towards the centre of the colony, quiescent, but viable cells sustain processes such as transport of water, solutes and organelles, and support the concerted genesis of specialized reproductive structures. a survey of chemical signals controlling changes in hyphal growth and branching of aspergillus nid ... | 2009 | 19799635 |
| remediation of arsenic in soil by aspergillus nidulans isolated from an arsenic-contaminated site. | high concentrations of heavy metals, such as arsenic, in soils have potential long-term environmental and health consequences due to their persistence in the environment and their associated toxicity to biological organisms. aspergillus nidulans isolated from arsenic-contaminated soil has the potential to remove arsenic from soil. the isolated resistant strain showed resistance up to 500 ppm and the mean weight was found to be 1.309 g. the main objective of this research was to study the improve ... | 2009 | 19803330 |
| study of codon bias perspective of fungal xylanase gene by multivariate analysis. | fungal xylanases has important applications in food, baking, pulp and paper industries in addition to various other industries. xylanases are produced extensively by both bacterial and fungal sources and has tremendous potential of being active at extremes of temperature and ph. in the present study an effort has been made to explore the codon bias perspective of this potential enzyme using bioinformatics tools. multivariate analysis has been used as a tool to study codon bias perspectives of xy ... | 2009 | 19759864 |
| in vivo analysis of the functions of gamma-tubulin-complex proteins. | to enhance our understanding of the function(s) of gamma-tubulin-complex proteins (gcps), we identified and analyzed the functions of the aspergillus nidulans homologs of gcp2-gcp6 (here designated gcpb-gcbf). the gamma-tubulin small complex (gamma-tusc) components, gamma-tubulin, gcpb and gcpc, are essential for viability and mitotic spindle formation, whereas gcpd-gcpf are not essential for viability, spindle formation or sexual reproduction. gcpd-gcpf function in reducing the frequency of chr ... | 2009 | 19861490 |
| using a new gpi-anchored-protein identification system to mine the protein databases of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae. | computational approaches provide valuable information to start experimental surveys identifying glycosylphosphatidylinositol (gpi)-anchored proteins in protein sequence databases. we developed a new sequence-based identification system that uses an optimized classifier based on a support vector machine (svm) algorithm to recognize appropriate cooh-terminal sequences and uses a classifier implementing a simple majority voting strategy to recognize appropriate nh2-terminal sequences. the svm class ... | 2009 | 19940384 |
| a key role for vesicles in fungal secondary metabolism. | eukaryotes have evolved highly conserved vesicle transport machinery to deliver proteins to the vacuole. in this study we show that the filamentous fungus aspergillus parasiticus employs this delivery system to perform new cellular functions, the synthesis, compartmentalization, and export of aflatoxin; this secondary metabolite is one of the most potent naturally occurring carcinogens known. here we show that a highly pure vesicle-vacuole fraction isolated from a. parasiticus under aflatoxin-in ... | 2009 | 19889978 |
| selection to sequence: opportunities in fungal genomics. | 2009 | 20025617 | |
| modelling the survival of escherichia coli o157:h7 on raw portioned tomatoes, inoculated with aspergillus fumigatus and emericella nidulans. | the metabiotic interactions occurring among two fungi (aspergillus fumigatus and emericella nidulans) and escherichia coli o157:h7 on raw portioned tomatoes were studied. tomatoes, preinoculated with the moulds and inoculated with the pathogen, were packaged in air and stored at 4, 8 and 12( composite function)c for 9 days; pathogen cell number and ph were monitored throughout the storage and the data were modeled using three different equations (geeraerd, weibull, and modified weibull), to asse ... | 2009 | 20037729 |
| inhibition of histone deacetylase causes reduction of appressorium formation in the rice blast fungus magnaporthe oryzae. | post-translational modifications (ptms) are important for cellular functions. the regulation of histone acetyltransferases (hats) and histone deacetylases (hdacs) is one of important ptms for epigenetic control, protein activity and protein stability. the regulation of acetylation of the n-terminal histone tails of core histone affects gene expression. two class i hdac genes and two class ii hdac genes have been identified in the magnaporthe oryzae genome. treatment with rpd3/hda1 family (classi ... | 2009 | 20118613 |
| [recent advances in the study of amorpha-4,11-diene synthase and its metabolic engineering]. | amorpha-4,11-diene synthase (ads) can convert farnesyl pyrophosphate (fpp) to amorpha-4, 11-diene, a precursor of artemisinin. ads plays an important role in the biosynthesis of artemisinin. this review summarizes the molecular biology and metabolic engineering study of ads in recent years. the genomic dna and its cdna sequences of amorpha-4, 11-diene synthase were cloned from artemisia annua l. the cdna encoding amorpha-4, 11-diene synthase contains a 1 641 bp open reading frame coding for 546 ... | 2009 | 21351463 |
| induction of cell wall thickening by the antifungal compound dihydromaltophilin disrupts fungal growth and is mediated by sphingolipid biosynthesis. | dihydromaltophilin (heat-stable antifungal factor [hsaf]) is an antifungal metabolite produced in lysobacter enzymogenes biocontrol strain c3. this compound induces cell wall thickening in aspergillus nidulans. here we show that the cell wall thickening is a general response to hsaf in diverse fungal species. in the a. nidulans model, the thickened cell wall negatively affects hyphal growth. growth of hsaf-pre-treated hyphae failed to resume at hyphal tips with thick cell wall and the actin cabl ... | 2009 | 21462551 |
| mononucleotide repeats represent an important source of polymorphic microsatellite markers in aspergillus nidulans. | in fungi, microsatellites occur less frequently throughout the genome and tend to be less polymorphic compared with other organisms. most studies that develop microsatellites for fungi focus on dinucleotide and trinucleotide repeats, and thus mononucleotide repeats, which are much more abundant in fungal genomes, may represent an overlooked resource. this study examined the relative probabilities of polymorphism in mononucleotide, dinucleotide and trinucleotide repeats in aspergillus nidulans. a ... | 2009 | 21564695 |
| mechanism of sterigmatocystin biosynthesis regulation by ph in aspergillus nidulans. | external ph constitutes one of the most important environmental factors that control growth, metabolism and differentiation in microorganisms, including fungi. we have analyzed the effect of external ph on sterigmatocystin biosynthesis in aspergillus nidulans. it was observed in repeated experiments that alkaline ph, in opposition to acid ph, increased sterigmatocystin production and the transcript levels of aflr, the master gene that regulates expression of the sterigmatocystin cluster in a. ni ... | 2009 | 24031444 |
| molecular genetics of emericella nidulans sexual development. | many aspergilli that belongs to ascomycetes have sexuality. in a homothallic or self-fertile fungus, a number of fruiting bodies or cleistothecia are formed in a thallus grown from a single haploid conidia or ascospores. genome-sequencing project revealed that two mating genes (mat) encoding the regulatory proteins that are necessary for controlling partner recognition in heterothallic fungi were conserved in most aspergilli. the mat gene products in some self-fertile species were not required f ... | 2009 | 23983529 |
| cytological characterization of an aspergillus nidulans mutant from a strain with chromosomic duplication. | a development mutant, named v103, was obtained spontaneously from the a strain of a. nidulans. the a strain contains a duplicated segment of chromosome i that has undergone translocation to chromosome ii (i ii). it is mitotically unstable and generates phenotypically deteriorated types, some with enhanced stability. the deteriorated variants of a. nidulans show abnormal development, exhibiting slower colony growth, variations in colony pigmentation and changes in conidiophore structure. the alte ... | 2010 | 24031489 |
| meab-dependent nutrition sensing regulates autolysis in carbon starved aspergillus nidulans cultures. | carbon starvation induced autolysis is an active process of self-digestion and is under complex regulation in aspergillus nidulans. in this study we investigated how autolysis depends on the composition of the culture medium, especially on the presence of yeast extract. we demonstrated that the rate of autolytic cell wall degradation as well as the extracellular chitinase and proteinase productions significantly decreased in the presence of this nutrient. the effect of yeast extract on carbon st ... | 2010 | 23100816 |
| [comparison of genomes between aspergillus nidulans and 30 filamentous ascomycetes]. | to investigate the conserved homologs of filamentous ascomycetes genomes, the local fungal genome database used in this analysis was established, which consisted of 31 latest and complete genome data publicly available on the internet. an expectation value cutoff of 0.1 was used to identify significant hits. each complete gene set of the query genome aspergillus nidulans genome with 10,560 annotated genes was splitted into individual fasta files with seqverter and then compared separately agains ... | 2010 | 21513172 |
| laea control of velvet family regulatory proteins for light-dependent development and fungal cell-type specificity. | vea is the founding member of the velvet superfamily of fungal regulatory proteins. this protein is involved in light response and coordinates sexual reproduction and secondary metabolism in aspergillus nidulans. in the dark, vea bridges velb and laea to form the velb-vea-laea (velvet) complex. the vea-like protein velb is another developmental regulator, and laea has been known as global regulator of secondary metabolism. in this study, we show that velb forms a second light-regulated developme ... | 2010 | 21152013 |
| phg/psilbaγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihprna - triggering in the mycorrhizal fungus laccaria bicolor. | psilbaγ silencing vector was constructed for efficient rna silencing triggering in the model mycorrhizal fungus laccaria bicolor. this cloning vector carries the agaricus bisporus gpdii promoter, two multiple cloning sites separated by a l. bicolor nitrate reductase intron and the aspergillus nidulans trpc terminator. psilbaγ allows an easy oriented two-step pcr cloning of hairpin sequences to be expressed in basidiomycetes. with one further cloning step into phg, a pcambia1300-based binary vect ... | 2010 | 21255319 |
| gene knockdown by ihprna-triggering in the ectomycorrhizal basidiomycete fungus laccaria bicolor. | ectomycorrhiza (ecm) is a mutualistic association between fungi and the roots of the vast majority of trees. these include numerous ecologically and economically relevant species and the participating fungal symbionts are predominantly filamentous basidiomycetes. in natural ecosystems the plant nutrient uptake from soil takes place via the extraradical mycelia of these ecm mycosimbionts as a trade for plant photosyntates. the symbiotic phase in the life cycle of ecm basidiomycetes is the dikaryo ... | 2010 | 21326837 |
| adaptative and developmental responses to stress in aspergillus nidulan. | development in the ascomycete a. nidulans is principally determined by environmental signals. adaptability to oxidative stimuli can derive in changes of growth patterns and /or the activation of sexual or asexual reproductive cycles but this model fungus might also respond to high osmotic or salt concentrations, the redox state, the availability and quantity of carbon or nitrogen sources and the degree or quality of illumination. since each cell within the colony follows a single morphogenetic p ... | 2010 | 21235506 |
| [screening and application of phosphate-dissolving microorganism suitable for corn production]. | to obtain phosphate-dissolving strains which can be suitable for corn production. | 2010 | 21365915 |
| kin3 protein, a nima-related kinase of saccharomyces cerevisiae, is involved in dna adduct damage response. | kin3 is a nonessential serine/threonine protein kinase of the budding yeast saccharomyces cerevisiae with unknown cellular role. it is an ortholog of the aspergillus nidulans protein kinase nima (never-in mitosis, gene a), which is involved in the regulation of g2/m phase progression, dna damage response and mitosis. the aim of this study was to determine whether kin3 is required for proper checkpoint activation and dna repair. here we show that kin3 gene deficient cells present sensitivity and ... | 2010 | 21577056 |
| enzymatic synthesis of β-xylosyl-oligosaccharides by transxylosylation using two β-xylosidases of glycoside hydrolase family 3 from aspergillus nidulans fgsc a4. | two β-xylosidases of glycoside hydrolase family 3 (gh 3) from aspergillus nidulans fgsc a4, bxla and bxlb were produced recombinantly in pichia pastoris and secreted to the culture supernatants in yields of 16 and 118 mg/l, respectively. bxla showed about sixfold higher catalytic efficiency (k(cat)/k(m)) than bxlb towards para-nitrophenyl β-d-xylopyranoside (pnpx) and β-1,4-xylo-oligosaccharides (degree of polymerisation 2-6). for both enzymes k(cat)/k(m) decreased with increasing β-1,4-xylo-oli ... | 2010 | 21215963 |
| simple identification of vea1 mutation in aspergillus nidulans. | the vea gene plays an important role in development of a homothallic filamentous fungus aspergillus nidulans. the vea1 phenotype can be difficult to distinguish from the wild-type vea. despite the importance of the vea allele, no efficient identification method has been reported besides dna sequencing. here, we present simple physiological and molecular biological ways to distinguish between the vea wild-type and vea1 allele. the novel approaches, which involve incubation in the presence of oxal ... | 2010 | 21221952 |
| penicillium marneffei actin expression during phase transition, oxidative stress, and macrophage infection. | penicillium marneffei is an opportunistic fungal pathogen that exhibits thermally regulated dimorphism. at 25°c, this fungus grows vegetatively as mycelia, but at 37°c or upon invasion of a host, a fission yeast form is established. yet, despite increased numbers of molecular studies involving this fungus, the role of p. marneffei stress response-related proteins is not well characterized. actin is one of the proteins that have been proposed to play a role not only in cell transition, but also i ... | 2010 | 21088905 |
| a set of aspartyl protease-deficient strains for improved expression of heterologous proteins in kluyveromyces lactis. | secretion of recombinant proteins is a common strategy for heterologous protein expression using the yeast kluyveromyces lactis. however, a common problem is degradation of a target recombinant protein by secretory pathway aspartyl proteases. in this study, we identified five putative pfam00026 aspartyl proteases encoded by the k. lactis genome. a set of selectable marker-free protease deletion mutants was constructed in the prototrophic k. lactis gg799 industrial expression strain background us ... | 2010 | 21166768 |
| recruitment of the inhibitor cand1 to the cullin substrate adaptor site mediates interaction to the neddylation site. | cand1 inhibits cullin ring ubiquitin ligases by binding unneddylated cullins. the cand1 n-terminus blocks the cullin neddylation site, whereas the c-terminus inhibits cullin adaptor interaction. these cand1 binding sites can be separated into two functional polypeptides which bind sequentially. c-terminal cand1 can directly bind to unneddylated cullins in the nucleus without blocking the neddylation site. the smaller n-terminal cand1 cannot bind to the cullin neddylation region without c-termina ... | 2010 | 21119001 |
| the aspergillus nidulans nuca(endog) homologue is not involved in cell death. | upon apoptosis induction, translocation of mammalian mitochondrial endonuclease g (endog) to the nucleus coincides with large-scale dna fragmentation. here, we describe for the first time a homologue of endog in filamentous fungi by investigating if the aspergillus nidulans homologue of the endog gene, named nuca(endog), is being activated during farnesol-induced cell death. our results suggest that nuca is not involved in cell death, but it plays a role in the dna-damaging response in a. nidula ... | 2010 | 21131437 |
| involvement of the aspergillus nidulans protein kinase c with farnesol tolerance is related to the unfolded protein response. | previously, we demonstrated that the aspergillus nidulans calc2 mutation in protein kinase c pkca was able to confer tolerance to farnesol (foh), an isoprenoid that has been shown to inhibit proliferation and induce apoptosis. here, we investigate in more detail the role played by a. nidulans pkca in foh tolerance. we demonstrate that pkca overexpression during foh exposure causes increased cell death. foh is also able to activate several markers of endoplasmic reticulum (er) stress and the unfo ... | 2010 | 21091509 |
| adaptative and developmental responses to stress in aspergillus nidulan. | development in the ascomycete a. nidulans is principally determined by environmental signals. adaptability to oxidative stimuli can derive in changes of growth patterns and /or the activation of sexual or asexual reproductive cycles but this model fungus might also respond to high osmotic or salt concentrations, the redox state, the availability and quantity of carbon or nitrogen sources and the degree or quality of illumination. since each cell within the colony follows a single morphogenetic p ... | 2010 | 21190514 |
| horizontal transfer of a large and highly toxic secondary metabolic gene cluster between fungi. | genes involved in intermediary and secondary metabolism in fungi are frequently physically linked or clustered. for example, in aspergillus nidulans the entire pathway for the production of sterigmatocystin (st), a highly toxic secondary metabolite and a precursor to the aflatoxins (af), is located in a ∼54 kb, 23 gene cluster. we discovered that a complete st gene cluster in podospora anserina was horizontally transferred from aspergillus. phylogenetic analysis shows that most podospora cluster ... | 2010 | 21194949 |
| crystallization and preliminary x-ray crystallographic analysis of the nmra-like ddb_g0286605 protein from the social amoeba dictyostelium discoideum. | the ddb_g0286605 gene product from dictyostelium discoideum, an nmra-like protein that belongs to the short-chain dehydrogenase/reductase family, has been crystallized by the hanging-drop vapour-diffusion method at 295 k. a 1.64 å resolution data set was collected using synchrotron radiation. the ddb_g0286605 protein crystals belonged to space group p2(1), with unit-cell parameters a=67.598, b=54.935, c=84.219 å, β = 109.620°. assuming the presence of two molecules in the asymmetric unit, the so ... | 2010 | 21206034 |
| transcriptome analysis of nitrate assimilation in aspergillus nidulans reveals connections to nitric oxide metabolism. | nitrate is a dominant form of inorganic nitrogen (n) in soils and can be efficiently assimilated by bacteria, fungi and plants. we studied here the transcriptome of the short-term nitrate response using assimilating and non-assimilating strains of the model ascomycete aspergillus nidulans. among the 72 genes positively responding to nitrate, only 18 genes carry binding sites for the pathway-specific activator nira. forty-five genes were repressed by nitrate metabolism. because nira(-) strains ar ... | 2010 | 20969648 |
| microbiological analysis of a mummy from the archeological museum in zagreb. | in this paper we report the results of the microbiological analysis of the samples taken from the mummy from the collection of the archaeological museum in zagreb, croatia. samples were taken from specific places such as oral, orbital, abdominal cavity and bandages surrounding the mummy, and analyzed in department of microbiology and hospital infections in university hospital "dubrava" in zagreb and in national reference laboratory for systemic mycoses of croatian national institute of public he ... | 2010 | 20977065 |
| an ortholog of fara of aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast yarrowia lipolytica. | the yeast yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. to identify a gene(s) regulating fatty acid utilization in y. lipolytica, we first studied homologous genes to oaf1 and pip2 of saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. we next characterized a y. lipolytica gene, por1 (primary oleate regulator 1), an ortholog of fara encoding a transcriptional activator that regulates fatty acid utilizatio ... | 2010 | 20977884 |
| type 2c protein phosphatases in fungi. | type 2c ser/thr phosphatases are a remarkable class of protein phosphatases, which are conserved in eukaryotes and involved in a large variety of functional processes. unlike in other ser/thr phosphatases, the catalytic polypeptide is not usually associated with regulatory subunits, and functional specificity is achieved by encoding multiple isoforms. for fungi, most information comes from the study of type 2c protein phosphatase (pp2c) enzymes in saccharomyces cerevisiae, where seven pp2c-encod ... | 2010 | 21076010 |
| global gene expression analysis of aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia. | hypoxia imposes a challenge upon most filamentous fungi that require oxygen for proliferation. here, we used whole genome dna microarrays to investigate global transcriptional changes in aspergillus nidulans gene expression after exposure to hypoxia followed by normoxia. aeration affected the expression of 2,864 genes (27% of the total number of genes in the fungus), of which 50% were either induced or repressed under hypoxic conditions. up-regulated genes included those for glycolysis, ethanol ... | 2010 | 20878186 |
| hapx-mediated adaption to iron starvation is crucial for virulence of aspergillus fumigatus. | iron is essential for a wide range of cellular processes. here we show that the bzip-type regulator hapx is indispensable for the transcriptional remodeling required for adaption to iron starvation in the opportunistic fungal pathogen aspergillus fumigatus. hapx represses iron-dependent and mitochondrial-localized activities including respiration, tca cycle, amino acid metabolism, iron-sulfur-cluster and heme biosynthesis. in agreement with the impact on mitochondrial metabolism, hapx-deficiency ... | 2010 | 20941352 |
| hypertonic conditions trigger transient plasmolysis, growth arrest and blockage of transporter endocytosis in aspergillus nidulans and saccharomyces cerevisiae. | by using aspergillus nidulans strains expressing functional gfp-tagged transporters under hypertonic conditions, we noticed the rapid appearance of cortical, relatively static, fluorescent patches (0.5-2.3 μm). these patches do not correspond to transporter microdomains as they co-localize with other plasma membrane-associated molecules, such as the pleckstrin homology (ph) domain and the ssoa t-snare, or the lipophilic markers fm4-64 and filipin. in addition, they do not show characteristics of ... | 2010 | 20919858 |
| suppressor mutagenesis identifies a velvet complex remediator of aspergillus nidulans secondary metabolism. | fungal secondary metabolites (sm) are bioactive compounds that are important in fungal ecology and, moreover, both harmful and useful in human endeavors (e.g., as toxins and pharmaceuticals). recently a nuclear heterocomplex termed the velvet complex, characterized in the model ascomycete aspergillus nidulans, was found to be critical for sm production. deletion of two members of the velvet complex, laea and vea, results in near loss of sm and defective sexual spore production in a. nidulans and ... | 2010 | 20935144 |
| fungal tyrosine betaine, a novel secondary metabolite from conidia of entomopathogenic metarhizium spp. fungi. | fungi, including the entomopathogenic deuteromycete metarhizium anisopliae, produce a wide diversity of secondary metabolites that either can be secreted or stored in specific developmental structures, e.g., conidia. some secondary metabolites, such as pigments, polyols and mycosporines, are associated with pathogenicity and/or fungal tolerance to several stress-inducing environmental factors, including temperature and solar radiation extremes. extracts of m. anisopliae var. anisopliae (strain e ... | 2010 | 20943158 |
| functional characterization of a new member of the cdk9 family in aspergillus nidulans. | cdk9-like kinases in complex with t-type cyclins are essential components of the eukaryotic transcription elongation machinery. the full spectrum of cdk9/cyclin t targets, as well as the specific consequences of phosphorylations, is still largely undefined. we identify and characterize here a cdk9 kinase (ptka) in the filamentous ascomycete aspergillus nidulans. deletion of ptka had a lethal effect in later stages of vegetative growth and completely impeded asexual development. overexpression of ... | 2010 | 20952582 |
| activation of a silent fungal polyketide biosynthesis pathway through regulatory cross talk with a cryptic nonribosomal peptide synthetase gene cluster. | filamentous fungi produce numerous natural products that constitute a consistent source of potential drug leads, yet it seems that the majority of natural products are overlooked since most biosynthesis gene clusters are silent under standard cultivation conditions. screening secondary metabolite genes of the model fungus aspergillus nidulans, we noted a silent gene cluster on chromosome ii comprising two nonribosomal peptide synthetase (nrps) genes, inpa and inpb, flanked by a regulatory gene t ... | 2010 | 20952652 |
| novel mutations reveal two important regions in aspergillus nidulans transcriptional activator metr. | expression of the sulfur assimilation pathway in aspergillus nidulans is under control of sulfur metabolite repression, which is composed of scon genes encoding subunits of ubiquitin ligase and the metr gene coding for a transcriptional activator. in this paper we report three dominant suppressors of methionine requirement isolated from a metb3 diploid strain. all three mutations lead to the substitution of phenylalanine 48 by serine or leucine in the conserved n-terminal region of the metr prot ... | 2010 | 20955810 |
| aspergillus nidulans asexual development: making the most of cellular modules. | asexual development in aspergillus nidulans begins in superficial hyphae as the programmed emergence of successive pseudohyphal modules, collectively known as the conidiophore, and is completed by a layer of specialized cells (phialides) giving rise to chains of aerial spores. a discrete number of regulatory factors present in hyphae play different stage-specific roles in pseudohyphal modules, depending on their cellular localization and protein-protein interactions. their multiple roles include ... | 2010 | 21035346 |
| the cop9 signalosome mediates transcriptional and metabolic response to hormones, oxidative stress protection and cell wall rearrangement during fungal development. | the cop9 signalosome complex (csn) is a crucial regulator of ubiquitin ligases. defects in csn result in embryonic impairment and death in higher eukaryotes, whereas the filamentous fungus aspergillus nidulans survives without csn, but is unable to complete sexual development. we investigated overall impact of csn activity on a. nidulans cells by combined transcriptome, proteome and metabolome analysis. absence of csn5/csne affects transcription of at least 15% of genes during development, inclu ... | 2010 | 21062371 |
| bioinspired deposition of tio2 thin films induced by hydrophobins. | the deposition of ceramic thin films from aqueous solutions at low temperature using biopolymers as templates has attracted much attention due to economic and environmental benefits. titanium dioxide is one of the most attractive functional materials and shows a wide range of applications across vastly different areas because of its unique chemical, optical, and electrical properties. in the present work, we deposited smooth, nanocrystalline titania thin films by an aqueous deposition method on ... | 2010 | 20121159 |
| [guideline based treatment of invasive aspergillosis]. | invasive fungus infections caused by aspergillus spp. occur most frequently in immunocompromised patients. a high infection-associated death rate of up to and over 50% is attributed even today to these fungi. the disease in humans is caused mainly by aspergillus fumigatus, aspergillus flavus and aspergillus niger. other species, for example, aspergillus terreus or aspergillus nidulans are quantitatively less prevalent. evidence based treatment of invasive aspergillosis has become safer and more ... | 2010 | 20433655 |
| atfa bzip-type transcription factor regulates oxidative and osmotic stress responses in aspergillus nidulans. | the aim of the study was to demonstrate that the bzip-type transcription factor atfa regulates different types of stress responses in aspergillus nidulans similarly to atf1, the orthologous 'all-purpose' transcription factor of schizosaccharomyces pombe. heterologous expression of atfa in a s. pombe deltaatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation ... | 2010 | 20131067 |
| heterochromatic marks are associated with the repression of secondary metabolism clusters in aspergillus nidulans. | fungal secondary metabolites are important bioactive compounds but the conditions leading to expression of most of the putative secondary metabolism (sm) genes predicted by fungal genomics are unknown. here we describe a novel mechanism involved in sm-gene regulation based on the finding that, in aspergillus nidulans, mutants lacking components involved in heterochromatin formation show de-repression of genes involved in biosynthesis of sterigmatocystin (st), penicillin and terrequinone a. durin ... | 2010 | 20132440 |
| the concerted action of bzip and cmyb transcription factors flbb and flbd induces brla expression and asexual development in aspergillus nidulans. | fungi are capable of generating diverse cell types through developmental processes that stem from hyphae, acting as pluripotent cells. the formation of mitospores on emergence of hyphae to the air involves the participation of transcription factors, which co-ordinate the genesis of new cell types, eventually leading to spore formation. in this investigation, we show that bzip transcription factor flbb, which has been attributed to participate in transducing the aerial stimulus signal, activates ... | 2010 | 20132447 |
| analysis of a novel calcium auxotrophy in aspergillus nidulans. | in aspergillus nidulans a combination of null mutations in hala, encoding a protein kinase, and slta, encoding a zinc-finger transcription factor having no yeast homologues, results in an elevated calcium requirement ('calcium auxotrophy') without impairing net calcium uptake. slta(-) (+/-hala(-)) mutations result in hypertrophy of the vacuolar system. in hala(-)slta(-) (and slta(-)) strains, transcript levels for pmca and pmcb, encoding vacuolar ca(2+)-atpase homologues, are highly elevated, su ... | 2010 | 20438880 |
| in vitro susceptibility testing in aspergillus species: an update. | aspergillus species are the most common causes of invasive mold infections in immunocompromised patients. the introduction of new antifungal agents and recent reports of resistance emerging during treatment of aspergillus infections have highlighted the need for in vitro susceptibility testing. various testing procedures have been proposed, including macro- and micro-dilution, disk diffusion, etest (ab biodisk, sweden) and other commercial tests. although aspergillus species are generally suscep ... | 2010 | 20441550 |
| molecular genetic analysis of the orsellinic acid/f9775 gene cluster of aspergillus nidulans. | f-9775a and f-9775b are cathepsin k inhibitors that arise from a chromatin remodelling deletant strain of aspergillus nidulans. a polyketide synthase gene has been determined to be responsible for their formation and for the simpler, archetypical polyketide orsellinic acid. we have discovered simple culture conditions that result in the production of the three compounds, and this facilitates analysis of the genes responsible for their synthesis. we have now analysed the f9775/orsellinic acid gen ... | 2010 | 20174687 |
| regulation of septum formation by the bud3-rho4 gtpase module in aspergillus nidulans. | the ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. the bud site selection system of saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. however, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. here, we describe the functional characterization and localization of the aspergill ... | 2010 | 20176976 |
| expression and purification of a functional uric acid-xanthine transporter (uapa). | the nucleobase-ascorbate transporters (nats) family includes carriers with fundamental functions in uptake of key cellular metabolites, such as uric acid or vitamin c. the best studied example of a nat transporter is the uric acid-xanthine permease (uapa) from the model ascomycete aspergillus nidulans. detailed genetic and biochemical analyses have revealed much about the mechanism of action of this protein; however, the difficulties associated with handling eukaryotic membrane proteins have lim ... | 2010 | 20153431 |
| metabolic and developmental effects resulting from deletion of the cita gene encoding citrate synthase in aspergillus nidulans. | citrate synthase is a central activity in carbon metabolism. it is required for the tricarboxylic acid (tca) cycle, respiration, and the glyoxylate cycle. in saccharomyces cerevisiae and arabidopsis thaliana, there are mitochondrial and peroxisomal isoforms encoded by separate genes, while in aspergillus nidulans, a single gene, cita, encodes a protein with predicted mitochondrial and peroxisomal targeting sequences (pts). deletion of cita results in poor growth on glucose but not on derepressin ... | 2010 | 20173036 |
| the conserved and divergent roles of carbonic anhydrases in the filamentous fungi aspergillus fumigatus and aspergillus nidulans. | carbon dioxide (co(2)) and its hydration product bicarbonate (hco(3)(-)) are essential molecules in various physiological processes of all living organisms. the reversible interconversion between co(2) and hco(3)(-) is in equilibrium. this reaction is slow without catalyst, but can be rapidly facilitated by zn(2+)-metalloenzymes named carbonic anhydrases (cas). to gain an insight into the function of multiple clades of fungal ca, we chose to investigate the filamentous fungi aspergillus fumigatu ... | 2010 | 20149101 |
| mitotic crossing-over induced by two commercial herbicides in diploid strains of the fungus aspergillus nidulans. | some herbicides are suspected of promoting teratogenic, carcinogenic and mutagenic events. detection of induced mitotic crossing-over has proven to be an indirect way of testing the carcinogenic properties of suspicious substances, because mitotic crossing-over is involved in the multistep process of carcinogenesis. we examined mitotic crossing-over induced by two commercial herbicides (diuron and trifluralin) in diploid strains of aspergillus nidulans based on the homozygotization index. low do ... | 2010 | 20198578 |
| proteome analysis of fungal and bacterial involvement in leaf litter decomposition. | fungi and bacteria are key players in the decomposition of leaf litter, but their individual contributions to the process and their interactions are still poorly known. we combined semi-quantitative proteome analyses (1-d page-lc-ms/ms) with qualitative and quantitative analyses of extracellular degradative enzyme activities to unravel the respective roles of a fungus and a bacterium during litter decomposition. two model organisms, a mesophilic gram-negative bacterium (pectobacterium carotovoru ... | 2010 | 20198641 |
| involvement of transposon-like elements in penicillin gene cluster regulation. | subtelomeric secondary metabolite (sm) gene clusters are frequently surrounded by dna repeats and transposon-like elements. the aspergillus nidulans penicillin cluster, 30kb from the telomere of chromosome vi, is bordered by such elements. deletions of penicillin telomere proximal and distal border regions resulted in decreased penicillin production. a 3.7kb distal region called pbia, consisting of the putative transposable element dna-2, was examined further where its replacement by a pyrg mark ... | 2010 | 20219692 |
| effect of light on growth, intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium. | the competence of the living creatures to sense and respond to light is well known. the effect of darkness and different color light quality on biomass, extracellular and intracellular pigment yield of five potent pigment producers monascus purpureus, isaria farinosa, emericella nidulans, fusarium verticillioides and penicillium purpurogenum, with different color shades such as red, pink, reddish brown and yellow, were investigated. incubation in total darkness increased the biomass, extracellul ... | 2010 | 20226375 |
| dynamic elements at both cytoplasmically and extracellularly facing sides of the uapa transporter selectively control the accessibility of substrates to their translocation pathway. | in the uapa uric acid-xanthine permease of aspergillus nidulans, subtle interactions between key residues of the putative substrate binding pocket, located in the tms8-tms9 loop (where tms is transmembrane segment), and a specificity filter, implicating residues in tms12 and the tms1-tms2 loop, are critical for function and specificity. by using a strain lacking all transporters involved in adenine uptake (deltaazga deltafcyb deltauapc) and carrying a mutation that partially inactivates the uapa ... | 2010 | 20188741 |
| distinct roles for caf1, ccr4, edc3 and cuta in the co-ordination of transcript deadenylation, decapping and p-body formation in aspergillus nidulans. | transcript degradation is a key step in gene regulation. in eukaryotes, mrna decay is generally initiated by removal of the poly(a) tail mediated by the ccr4-caf1-not complex. deadenylated transcripts are then rapidly degraded, primarily via the decapping-dependent pathway. components of this pathway can be localized into highly dynamic cytoplasmic foci, the mrna processing (p)-bodies. we have undertaken confocal fluorescence microscopy to monitor p-bodies in aspergillus nidulans. as in other or ... | 2010 | 20233300 |
| novel insights into the functional role of three protein arginine methyltransferases in aspergillus nidulans. | protein arginine methylation has been implicated in different cellular processes including transcriptional regulation by the modification of histone proteins. here we demonstrate significant in vitro activities and multifaceted specificities of aspergillus protein arginine methyltransferases (prmts) and we provide evidence for a role of protein methylation in mechanisms of oxidative stress response. we have isolated all three aspergillus prmts from fungal extracts and could assign significant hi ... | 2010 | 20338257 |
| interaction of the aspergillus nidulans microtubule-organizing center (mtoc) component apsb with gamma-tubulin and evidence for a role of a subclass of peroxisomes in the formation of septal mtocs. | peroxisomes are a diverse class of organelles involved in different physiological processes in eukaryotic cells. although proteins imported into peroxisomes carry a peroxisomal targeting sequence at the c terminus (pts1) or an alternative one close to the n terminus (pts2), the protein content of peroxisomes varies drastically. here we suggest a new class of peroxisomes involved in microtubule (mt) formation. eukaryotic cells assemble mts from distinct points in the cell. in the fungus aspergill ... | 2010 | 20348383 |
| antifungal activity of eugenol against penicillium, aspergillus, and fusarium species. | the antifungal activity of eugenol in a model system against aspergilli (aspergillus niger, aspergillus terreus, and emericella nidulans), penicilli (penicillium expansum, penicillium glabrum, and penicillium italicum), and fusaria (fusarium oxysporum and fusarium avenaceum) was investigated. minimum detection time (time to attain a colony diameter of 1 cm) and the kinetic parameters were evaluated. the effectiveness of the active compound seemed to be strain or genus dependent; 100 mg/liter rep ... | 2010 | 20537272 |
| a septin from the filamentous fungus a. nidulans induces atypical pseudohyphae in the budding yeast s. cerevisiae. | septins, novel cytoskeletal proteins, form rings at the bases of emerging round buds in yeasts and at the bases of emerging elongated hyphal initials in filamentous fungi. | 2010 | 20360845 |
| gdp-mannose transporter paralogues play distinct roles in polarized growth of aspergillus nidulans. | gdp-mannose transporters (gmt) carry gdp-mannose nucleotide sugars from the cytosol across the golgi apparatus membrane for use as substrates in protein glycosylation in plants, animals and fungi. genomes of some fungal species, such as the yeast saccharomyces cerevisiae, contain only one gene encoding a gmt, while others, including aspergillus nidulans, contain two (gmta and gmtb). we previously showed that cell wall integrity and normal hyphal morphogenesis in a. nidulans depend upon the funct ... | 2010 | 20361498 |
| asperfuranone from aspergillus nidulans inhibits proliferation of human non-small cell lung cancer a549 cells via blocking cell cycle progression and inducing apoptosis. | asperfuranone, a novel compound of genomic mining in aspergillus nidulans, was investigated for its anti-proliferative activity in human non-small cell lung cancer a549 cells. to identity the anti-cancer mechanism of asperfuranone, we assayed its effect on apoptosis, cell cycle distribution, and levels of p53, p21 waf1/cip1, fas/apo-1 receptor and fas ligand. enzyme-linked immunosorbent assay showed that the g0/g1 phase arrest might be due to p53-dependent induction of p21 waf1/cip1. an enhancem ... | 2010 | 20148857 |
| high-throughput screening of dna binding sites for transcription factor amyr from aspergillus nidulans using dna beads display system. | we established a high-throughput screening method for the dna binding sequence of a eukaryotic transcription factor by using the bead display system with emulsion pcr and flow cytometry and applied it for identifying a eukaryotic transcriptional activator amyr, which is known to regulate amylolytic gene expression in aspergillus species. segmented parts of the binding site of amyr were randomized to make a dna library on beads, onto which male-tagged amyr protein and fluorescent anti-male tag an ... | 2010 | 20471587 |
| deletion of the ring-finger peroxin 2 gene in aspergillus nidulans does not affect meiotic development. | peroxins are required for protein import into peroxisomes as well as for peroxisome biogenesis and proliferation. loss-of-function mutations in genes for the ring-finger peroxins pex2, pex10 and pex12 lead to a specific block in meiosis in the ascomycete podospora anserina. however, loss of protein import into peroxisomes does not result in this meiotic defect. therefore, it has been suggested that these peroxins have a specific function required for meiosis. to determine whether this role is co ... | 2010 | 20236327 |
| aspergillus nidulans contains six possible fatty acyl-coa synthetases with faab being the major synthetase for fatty acid degradation. | aspergillus nidulans can use a variety of fatty acids as sole carbon and energy sources via its peroxisomal and mitochondrial beta-oxidation pathways. prior to channelling the fatty acids into beta-oxidation, they need to be activated to their acyl-coa derivates. analysis of the genome sequence identified a number of possible fatty acyl-coa synthetases (fata, fatb, fatc, fatd, faaa and faab). faab was found to be the major long-chain synthetase for fatty acid degradation. faab was shown to local ... | 2010 | 20354844 |
| the fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts. | saccharomyces cerevisiae acquires a resistance to high, toxic levels of acetic acid by destabilizing fps1p, the plasma membrane aquaglyceroporin through which this acid - in its undissociated state - enters the cell. in this study, fps1p loss was shown to confer resistances to acetic acid, acrolein and allyl alcohol, not just in s. cerevisiae but also in the osmotolerant spoilage yeast zygosaccharomyces rouxii. however, in z. rouxii, the loss of fps1p severely compromised the use of acetamide an ... | 2010 | 20491941 |