PMID(sorted descending)
effect of light on growth, intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium.the competence of the living creatures to sense and respond to light is well known. the effect of darkness and different color light quality on biomass, extracellular and intracellular pigment yield of five potent pigment producers monascus purpureus, isaria farinosa, emericella nidulans, fusarium verticillioides and penicillium purpurogenum, with different color shades such as red, pink, reddish brown and yellow, were investigated. incubation in total darkness increased the biomass, extracellul ...201020226375
involvement of transposon-like elements in penicillin gene cluster regulation.subtelomeric secondary metabolite (sm) gene clusters are frequently surrounded by dna repeats and transposon-like elements. the aspergillus nidulans penicillin cluster, 30kb from the telomere of chromosome vi, is bordered by such elements. deletions of penicillin telomere proximal and distal border regions resulted in decreased penicillin production. a 3.7kb distal region called pbia, consisting of the putative transposable element dna-2, was examined further where its replacement by a pyrg mark ...201020219692
aspergillus nidulans udp-glucose-4-epimerase ugea has multiple roles in wall architecture, hyphal morphogenesis, and asexual development.aspergillus nidulans udp-glucose-4-epimerase ugea interconverts udp-glucose and udp-galactose and participates in galactose metabolism. the sugar moiety of udp-galactose is predominantly found as galactopyranose (galp, the six-membered ring form), which is the substrate for udp-galactopyranose mutase (encoded by ugma) to generate udp-galactofuranose (galf, the five-membered ring form) that is found in fungal walls. in a. fumigatus, galf residues appear to be important for virulence. the a. nidul ...201020211750
proteome analysis of fungal and bacterial involvement in leaf litter decomposition.fungi and bacteria are key players in the decomposition of leaf litter, but their individual contributions to the process and their interactions are still poorly known. we combined semi-quantitative proteome analyses (1-d page-lc-ms/ms) with qualitative and quantitative analyses of extracellular degradative enzyme activities to unravel the respective roles of a fungus and a bacterium during litter decomposition. two model organisms, a mesophilic gram-negative bacterium (pectobacterium carotovoru ...201020198641
mitotic crossing-over induced by two commercial herbicides in diploid strains of the fungus aspergillus nidulans.some herbicides are suspected of promoting teratogenic, carcinogenic and mutagenic events. detection of induced mitotic crossing-over has proven to be an indirect way of testing the carcinogenic properties of suspicious substances, because mitotic crossing-over is involved in the multistep process of carcinogenesis. we examined mitotic crossing-over induced by two commercial herbicides (diuron and trifluralin) in diploid strains of aspergillus nidulans based on the homozygotization index. low do ...201020198578
dynamic elements at both cytoplasmically and extracellularly facing sides of the uapa transporter selectively control the accessibility of substrates to their translocation the uapa uric acid-xanthine permease of aspergillus nidulans, subtle interactions between key residues of the putative substrate binding pocket, located in the tms8-tms9 loop (where tms is transmembrane segment), and a specificity filter, implicating residues in tms12 and the tms1-tms2 loop, are critical for function and specificity. by using a strain lacking all transporters involved in adenine uptake (deltaazga deltafcyb deltauapc) and carrying a mutation that partially inactivates the uapa ...201020188741
regulation of septum formation by the bud3-rho4 gtpase module in aspergillus nidulans.the ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. the bud site selection system of saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. however, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. here, we describe the functional characterization and localization of the aspergill ...201020176976
molecular genetic analysis of the orsellinic acid/f9775 gene cluster of aspergillus nidulans.f-9775a and f-9775b are cathepsin k inhibitors that arise from a chromatin remodelling deletant strain of aspergillus nidulans. a polyketide synthase gene has been determined to be responsible for their formation and for the simpler, archetypical polyketide orsellinic acid. we have discovered simple culture conditions that result in the production of the three compounds, and this facilitates analysis of the genes responsible for their synthesis. we have now analysed the f9775/orsellinic acid gen ...201020174687
metabolic and developmental effects resulting from deletion of the cita gene encoding citrate synthase in aspergillus nidulans.citrate synthase is a central activity in carbon metabolism. it is required for the tricarboxylic acid (tca) cycle, respiration, and the glyoxylate cycle. in saccharomyces cerevisiae and arabidopsis thaliana, there are mitochondrial and peroxisomal isoforms encoded by separate genes, while in aspergillus nidulans, a single gene, cita, encodes a protein with predicted mitochondrial and peroxisomal targeting sequences (pts). deletion of cita results in poor growth on glucose but not on derepressin ...201020173036
antimicrobial, wound healing and antioxidant activities of anthocephalus cadamba.anthocephalus cadamba (roxb.) miq. syn a. chinensis (lamk) a. rich (rubiaceae) is ethnomedicinally widely used in the form of paste by tribe in western ghats for treating skin diseases. in this context, antimicrobial potential of a. cadamba against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing. the alchoholic and aqueou ...200720161916
evaluation of performance and community dynamics of microorganisms during treatment of distillery spent wash in a three stage bioreactor.the ability of emericella nidulans var. lata, neurospora intermedia and bacillus sp. to treat distillery spent wash in a three stage bioreactor was investigated. process parameters were optimized in shake flask cultures with the individual strains before treatment of the effluent in a 15-l bioreactor. treatment was first carried out by the fungi followed by bacteria. the treated effluent showed significant reduction in color (82%) and cod (93%) after 30 h. metabolites formed after degradation of ...201020156685
expression and purification of a functional uric acid-xanthine transporter (uapa).the nucleobase-ascorbate transporters (nats) family includes carriers with fundamental functions in uptake of key cellular metabolites, such as uric acid or vitamin c. the best studied example of a nat transporter is the uric acid-xanthine permease (uapa) from the model ascomycete aspergillus nidulans. detailed genetic and biochemical analyses have revealed much about the mechanism of action of this protein; however, the difficulties associated with handling eukaryotic membrane proteins have lim ...201020153431
overexpression of bacterial ethylene-forming enzyme gene in trichoderma reesei enhanced the production of order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from pseudomonas syringae pv. glycinea were constructed. the target gene was respectively controlled by different promoters: cbh i promoter from trichoderma reesei cellobiohydrolases i gene, gpd promoter from aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase gene and pgk i promoter from t. reesei 3-phosphoglycerate kinase i gene. ...201020150979
the conserved and divergent roles of carbonic anhydrases in the filamentous fungi aspergillus fumigatus and aspergillus nidulans.carbon dioxide (co(2)) and its hydration product bicarbonate (hco(3)(-)) are essential molecules in various physiological processes of all living organisms. the reversible interconversion between co(2) and hco(3)(-) is in equilibrium. this reaction is slow without catalyst, but can be rapidly facilitated by zn(2+)-metalloenzymes named carbonic anhydrases (cas). to gain an insight into the function of multiple clades of fungal ca, we chose to investigate the filamentous fungi aspergillus fumigatu ...201020149101
asperfuranone from aspergillus nidulans inhibits proliferation of human non-small cell lung cancer a549 cells via blocking cell cycle progression and inducing apoptosis.asperfuranone, a novel compound of genomic mining in aspergillus nidulans, was investigated for its anti-proliferative activity in human non-small cell lung cancer a549 cells. to identity the anti-cancer mechanism of asperfuranone, we assayed its effect on apoptosis, cell cycle distribution, and levels of p53, p21 waf1/cip1, fas/apo-1 receptor and fas ligand. enzyme-linked immunosorbent assay showed that the g0/g1 phase arrest might be due to p53-dependent induction of p21 waf1/cip1. an enhancem ...201020148857
genotoxic activity of eucalyptus globulus essential oil in aspergillus nidulans diploid cells.eucalyptus globulus essential oil was evaluated for its genotoxic potential using a somatic segregation assay and a diploid strain of the fungus aspergillus nidulans, heterozygous for nutritional and conidia color markers. the main compounds of the current essential oil sample were eucalyptol (49.0 %), alpha-pinene (8.9), beta-pinene (1.5), globulol (6.9), alpha-eudesmol (1.12), spathulenol (1.42), gamma-cadinene (1.45), trans-beta-elemenone (1.23) and aromandendrene (2.3), totaling 74 % of oil. ...201020140715
characterization of the aspergillus nidulans monodictyphenone gene cluster.deletion of ccla, a component of the compass complex of aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. through a set of targeted deletions in a ccla deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. identification of an intermediate, endocrocin, from an mdphdelta strain suggests that mdph might encode a decarboxylase. furthermore, by replacing the promoter of mdpa (a putative aflj homolog) and ...201020139316
the concerted action of bzip and cmyb transcription factors flbb and flbd induces brla expression and asexual development in aspergillus nidulans.fungi are capable of generating diverse cell types through developmental processes that stem from hyphae, acting as pluripotent cells. the formation of mitospores on emergence of hyphae to the air involves the participation of transcription factors, which co-ordinate the genesis of new cell types, eventually leading to spore formation. in this investigation, we show that bzip transcription factor flbb, which has been attributed to participate in transducing the aerial stimulus signal, activates ...201020132447
heterochromatic marks are associated with the repression of secondary metabolism clusters in aspergillus nidulans.fungal secondary metabolites are important bioactive compounds but the conditions leading to expression of most of the putative secondary metabolism (sm) genes predicted by fungal genomics are unknown. here we describe a novel mechanism involved in sm-gene regulation based on the finding that, in aspergillus nidulans, mutants lacking components involved in heterochromatin formation show de-repression of genes involved in biosynthesis of sterigmatocystin (st), penicillin and terrequinone a. durin ...201020132440
atfa bzip-type transcription factor regulates oxidative and osmotic stress responses in aspergillus nidulans.the aim of the study was to demonstrate that the bzip-type transcription factor atfa regulates different types of stress responses in aspergillus nidulans similarly to atf1, the orthologous 'all-purpose' transcription factor of schizosaccharomyces pombe. heterologous expression of atfa in a s. pombe deltaatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation ...201020131067
bioinspired deposition of tio2 thin films induced by hydrophobins.the deposition of ceramic thin films from aqueous solutions at low temperature using biopolymers as templates has attracted much attention due to economic and environmental benefits. titanium dioxide is one of the most attractive functional materials and shows a wide range of applications across vastly different areas because of its unique chemical, optical, and electrical properties. in the present work, we deposited smooth, nanocrystalline titania thin films by an aqueous deposition method on ...201020121159
inhibition of histone deacetylase causes reduction of appressorium formation in the rice blast fungus magnaporthe modifications (ptms) are important for cellular functions. the regulation of histone acetyltransferases (hats) and histone deacetylases (hdacs) is one of important ptms for epigenetic control, protein activity and protein stability. the regulation of acetylation of the n-terminal histone tails of core histone affects gene expression. two class i hdac genes and two class ii hdac genes have been identified in the magnaporthe oryzae genome. treatment with rpd3/hda1 family (classi ...200920118613
photodynamic inactivation of conidia of the fungi metarhizium anisopliae and aspergillus nidulans with methylene blue and toluidine blue.antimicrobial photodynamic treatment (pdt) is a promising method that can be used to control localized mycoses or kill fungi in the environment. a major objective of the current study was to compare the conidial photosensitization of two fungal species (metarhizium anisopliae and aspergillus nidulans) with methylene blue (mb) and toluidine blue (tbo) under different incubation and light conditions. parameters examined were media, photosensitizer (ps) concentration and light source. pdt with mb a ...201020113427
molecular cloning, characterization and expression analysis of two beta-n-acetylhexosaminidase homologs of coccidioides posadasii.two full-length cdnas were isolated from coccidioides posadasii that encode two deduced proteins (cphex1 and cphex2) with homology to the glycosyl hydrolase 20 family of beta-n-acetylhexosaminidases. cphex1 consists of 595 amino acids, has a predicted molecular mass of 68 kda and shares the highest identity with the n-acetylhexosaminidase (naga) of aspergillus nidulans, while cphex2 consists of 603 amino acids, has a predicted molecular mass of 68.5 kda and shares the highest identity with nag1 ...201020109094
lipoperoxidation affects ochratoxin a biosynthesis in aspergillus ochraceus and its interaction with wheat aspergillus nidulans, aspergillus flavus, and aspergillus parasiticus, lipoperoxidative signalling is crucial for the regulation of mycotoxin biosynthesis, conidiogenesis, and sclerotia formation. resveratrol, which is a lipoxygenase (lox) and cyclooxygenase inhibitor, downmodulates the biosynthesis of ochratoxin a (ota) in aspergillus ochraceus. in the genome of a. ochraceus, a lox-like sequence (aoloxa; national center for biotechnology information (ncbi) accession number: dq087531) for a l ...201020101489
the transposon impala is activated by low temperatures: use of a controlled transposition system to identify genes critical for viability of aspergillus fumigatus.genes that are essential for viability represent potential targets for the development of anti-infective agents. however, relatively few have been determined in the filamentous fungal pathogen aspergillus fumigatus. a novel solution employing parasexual genetics coupled with transposon mutagenesis using the fusarium oxysporum transposon impala had previously enabled the identification of 20 essential genes from a. fumigatus; however, further use of this system required a better understanding of ...201020097738
design, synthesis and biological evaluation of carboxy analogues of arginine methyltransferase inhibitor 1 (ami-1).here we report the synthesis of a number of compounds structurally related to arginine methyltransferase inhibitor 1 (ami-1). the structural alterations that we made included: 1) the substitution of the sulfonic groups with the bioisosteric carboxylic groups; 2) the replacement of the ureidic function with a bis-amidic moiety; 3) the introduction of a n-containing basic moiety; and 4) the positional isomerization of the aminohydroxynaphthoic moiety. we have assessed the biological activity of th ...201020091730
mechanism of de novo branched-chain amino acid synthesis as an alternative electron sink in hypoxic aspergillus nidulans cells.although branched-chain amino acids are synthesized as building blocks of proteins, we found that the fungus aspergillus nidulans excretes them into the culture medium under hypoxia. the transcription of predicted genes for synthesizing branched-chain amino acids was upregulated by hypoxia. a knockout strain of the gene encoding the large subunit of acetohydroxy acid synthase (ahas), which catalyzes the initial reaction of the synthesis, required branched-chain amino acids for growth and excrete ...201020081005
identification of possible targets of the aspergillus fumigatus crz1 homologue, crza.calcineurin, a serine/threonine-specific protein phosphatase, plays an important role in the control of cell morphology and virulence in fungi. calcineurin regulates localization and activity of a transcription factor called crz1. recently, we characterize aspergillus fumigatus crz1 homologue, afcrza. here, we investigate which pathways are influenced by a. fumigatus afcrza during a short pulse of calcium by comparatively determining the transcriptional profile of a. fumigatus wild type and delt ...201020078882
reaction coordinate of isopenicillin n synthase: oxidase versus oxygenase activity.isopenicillin n synthase (ipns) can have both oxidase and oxygenase activity depending on the substrate. for the native substrate, acv, oxidase activity exists; however, for the substrate analogue acov, which lacks an amide nitrogen, ipns exhibits oxygenase activity. the potential energy surfaces for the o-o bond elongation and cleavage were calculated for three different reactions: homolytic cleavage via traditional fenton chemistry, heterolytic cleavage, and nucleophilic attack. these surfaces ...201020078029
regulation of ustilago maydis dimorphism, sporulation, and pathogenic development by a transcription factor with a highly conserved apses ustilago maydis, the causal agent of corn smut, the morphological transition from yeast to filamentous growth is inextricably linked to pathogenicity; budding haploid cells are saprobic and, upon mating of compatible strains, the fungus converts to dikaryotic filamentous growth and obligate parasitism. the filamentous dikaryon proliferates in the host plant, inducing tumor formation and undergoing additional morphological changes that eventually result in the production of melanized diploid t ...201020064064
reduced expression of the o-mannosyltransferase 2 (afpmt2) leads to deficient cell wall and abnormal polarity in aspergillus fumigatus.protein o-mannosyltransferases (pmts) initiate o-mannosylation of secretory proteins, which are of fundamental importance in eukaryotes. the human fungal pathogen aspergillus fumigatus possesses three genes encoding for pmts, namely, afpmt1, afpmt2 and afpmt4. we have previously shown that lack of afpmt1 leads to a temperature-sensitive phenotype featured with severe defects in hyphal growth, conidiation, cell wall integrity and morphology at elevated temperatures. in this study, a conditional m ...201020053626
parp is involved in replicative aging in neurospora crassa.modification of proteins by the addition of poly(adp-ribose) is carried out by poly(adp-ribose) polymerases (parps). parps have been implicated in a wide range of biological processes in eukaryotes, but no universal function has been established. a study of the aspergillus nidulans parp ortholog (prpa) revealed that the protein is essential and involved in dna repair, reminiscent of findings using mammalian systems. we found that a neurospora parp orthologue (npo) is dispensable for cell surviva ...201020045739
aoxa is a major peroxisomal long chain fatty acyl-coa oxidase required for beta-oxidation in a. nidulans.filamentous fungi can use a variety of fatty acids (fa) as sole carbon and energy sources. aspergillus nidulans has been shown to possess both peroxisomal and mitochondrial beta-oxidation pathways. in these studies, the major peroxisomal long chain fatty acyl coenzyme a oxidase aoxa was identified. aoxa was shown to be localised to peroxisomes and deletion of the aoxa gene leads to reduced growth on long chain fa, but not on short chain fa. aoxa is predicted to be part of the same peroxisomal be ...201020043225
modelling the survival of escherichia coli o157:h7 on raw portioned tomatoes, inoculated with aspergillus fumigatus and emericella nidulans.the metabiotic interactions occurring among two fungi (aspergillus fumigatus and emericella nidulans) and escherichia coli o157:h7 on raw portioned tomatoes were studied. tomatoes, preinoculated with the moulds and inoculated with the pathogen, were packaged in air and stored at 4, 8 and 12( composite function)c for 9 days; pathogen cell number and ph were monitored throughout the storage and the data were modeled using three different equations (geeraerd, weibull, and modified weibull), to asse ...200920037729
localization and function of calmodulin in live-cells of aspergillus nidulans.calmodulin (cam) is a small, eukaryotic protein that reversibly binds ca(2+). study of cam localization in genetically tractable organisms has yielded many insights into cam function. here, we described the dynamic localization of aspergillus nidulans cam (ancam) in live-cells by using recombination strains with homologous, single cross-over insertions at the target gene which placed the gfp fused copy under the inducible alca promoter and the rfp-cam integration under the native cam promoter. w ...201020034586
a cryptic role of a glycolytic-gluconeogenic enzyme (aldolase) in amino acid transporter turnover in aspergillus aspergillus nidulans the fbaa1013 mutation results in reduced or total loss of growth on glycolytic and gluconeogenic carbon sources, respectively. it also negatively affects growth on several amino acids (including l-proline, l-glutamate or l-aspartate) that the fungus can use as nitrogen source on glycolytic carbon sources. complementation of the fbaa1013 mutation using an a. nidulans genomic library resulted in cloning of the fbaa gene, which encodes a putative fructose 1,6-biphosphate ald ...201020026236
selection to sequence: opportunities in fungal genomics. 200920025617
the crystal structure of an lll-configured depsipeptide substrate analogue bound to isopenicillin n synthase.isopenicillin n synthase (ipns) is a non-heme iron(ii) oxidase, which catalyses the biosynthesis of isopenicillin n (ipn) from the tripeptide delta-l-alpha-aminoadipoyl-l-cysteinyl-d-valine (lld-acv) in a remarkable oxidative bicyclisation reaction. the natural substrate for ipns is the lld-configured tripeptide. lll-acv is not turned over by the enzyme, but inhibits turnover of the lld-tripeptide. the mechanism by which this inhibition takes place is not fully understood. recent studies have em ...201020024142
caspofungin etest endpoint for aspergillus isolates shows poor agreement with the reference minimum effective concentration.the clinical and laboratory standards institute (clsi) m38-a2 reference broth microdilution (bmd) method for the antifungal susceptibility testing of filamentous fungi now includes guidelines for testing echinocandin activity using the minimum effective concentration (mec) as the endpoint measurement. in this study, we compared the caspofungin etest mic on rpmi agar and mueller-hinton agar (supplemented with glucose and methylene blue [mgm]) to the bmd mec for 345 clinical aspergillus isolates, ...201020007389
formate-nitrite transporters: optimisation of expression, purification and analysis of prokaryotic and eukaryotic representatives.the formate-nitrite transporter family is composed of integral membrane proteins that possess six to eight alpha-helical transmembrane domains. genes encoding these proteins are observed widely in prokaryotic genomes as well as certain groups of lower eukaryotes. thus far, no structural information is available for this transporter family. towards this aim, and to provide protein for biophysical studies, overexpression of a prokaryotic (tpnirc, from the hyperthermophilic archaebacterium thermofi ...201020006708
transport-dependent endocytosis and turnover of a uric acid-xanthine this work we unmask a novel downregulation mechanism of the uric acid/xanthine transporter uapa, the prototype member of the ubiquitous nucleobase-ascorbate transporter family, directly related to its function. in the presence of substrates, uapa is endocytosed, sorted into the multivesicular body pathway and degraded in vacuoles. substrate-induced endocytosis, unlike ammonium-induced turnover, is absolutely dependent on uapa activity and several lines of evidence showed that the signal for i ...201020002879
the ccaat-binding complex coordinates the oxidative stress response in eukaryotes.the heterotrimeric ccaat-binding complex is evolutionary conserved in eukaryotic organisms. the corresponding aspergillus nidulans ccaat-binding factor (ancf) consists of the subunits hapb, hapc and hape. all of the three subunits are necessary for dna binding. here, we demonstrate that ancf senses the redox status of the cell via oxidative modification of thiol groups within the histone fold motif of hapc. mutational and in vitro interaction analyses revealed that two of these cysteine residues ...201019965775
erl1, a novel era-like gtpase from magnaporthe oryzae, is required for full root virulence and is conserved in the mutualistic symbiont glomus intraradices.comparative analyses of genome sequences from several plant-infecting fungi have shown conservation and expansion of protein families with plant disease-related functions. here, we show that this hypothesis can be extended to mutualistic symbiotic fungi. we have identified a gene encoding an era (escherichia coli ras)-like gtpase in the rice blast fungus magnaporthe oryzae and found that it is orthologous to the mature amino terminal part of the gin1 protein from the arbuscular mycorrhizal (am) ...201019958140
the properties of adaptive walks in evolving populations of fungus.the rarity of beneficial mutations has frustrated efforts to develop a quantitative theory of adaptation. recent models of adaptive walks, the sequential substitution of beneficial mutations by selection, make two compelling predictions: adaptive walks should be short, and fitness increases should become exponentially smaller as successive mutations fix. we estimated the number and fitness effects of beneficial mutations in each of 118 replicate lineages of aspergillus nidulans evolving for appr ...200919956798
septins aspa and aspc are important for normal development and limit the emergence of new growth foci in the multicellular fungus aspergillus nidulans.septins are cytoskeletal proteins found in fungi, animals, and microsporidia, where they form multiseptin complexes that act as scaffolds recruiting and organizing other proteins to ensure normal cell division and development. here we characterize the septins aspa and aspc in the multicellular, filamentous fungus aspergillus nidulans. mutants with deletions of aspa, aspc, or both aspa and aspc show early and increased germ tube and branch emergence, abnormal septation, and disorganized conidioph ...201019949047
using a new gpi-anchored-protein identification system to mine the protein databases of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae.computational approaches provide valuable information to start experimental surveys identifying glycosylphosphatidylinositol (gpi)-anchored proteins in protein sequence databases. we developed a new sequence-based identification system that uses an optimized classifier based on a support vector machine (svm) algorithm to recognize appropriate cooh-terminal sequences and uses a classifier implementing a simple majority voting strategy to recognize appropriate nh2-terminal sequences. the svm class ...200919940384
a novel motif in fungal class 1 histone deacetylases is essential for growth and development of aspergillus.acetylation of the n-terminal tails of core histones is an important regulatory mechanism in eukaryotic organisms. in filamentous fungi, little is known about the enzymes that modify histone tails. however, it is increasingly evident that histone deacetylases and histone acetyltransferases are critical factors for the regulation of genes involved in fungal pathogenicity, stress response, and production of secondary metabolites such as antibiotics or fungal toxins. here, we show that depletion of ...201019940017
protoplast isolation from cultured lichen usnea ghattensis, their fusion with protoplasts of aspergillus nidulans, fusant regeneration and production of usnic acid.protoplasts isolated from the mycobiont of a cultured lichen usnea ghattensis were fused with protoplasts of the fungus aspergillus nidulans in order to increase the growth rate of the cultured lichen mycobiont in vitro. the maximum protoplast yield (102 x 10(4)/g fresh cell mass) was reached in citrate buffer with 50 mmol/l 2-sulfanylethanol ('2-mercaptoethanol') containing 0.1 % novozym after 1.5 h at ph 5 and <or=25 degrees c. the increase in the concentration of the above effectors or the ad ...200919937214
proteomic analysis of early phase of conidia germination in aspergillus order to investigate proteins involved in early phase of conidia germination, proteomic analysis was performed using two-dimensional gel electrophoresis (2d-ge) in conjunction with maldi-tof mass spectrometry (ms). the expression levels of 241 proteins varied quantitatively with statistical significance (p<0.05) at the early phase of the germination stage. out of these 57 were identified by maldi-tof ms. through classification of physiological functions from conserved domain database analysis ...201019919853
unilateral ureteral obstruction caused by aspergillus, subgenus nidulantes in a patient on steroid therapy: a case report and review of the literature.urinary tract obstructions caused by aspergillus bezoars has been reported on rare occasions. we describe in this paper an unusual case caused by an isolate of the aspergillus nidulantes subgenus, and review the literature on 13 additional cases of ureteral obstruction due to renoureteric aspergillosis so as to provide the characteristics of this disease entity. our case presented with a unilateral ureteral obstruction and acute renal failure due to aspergillus bezoars. the patient was immunocom ...201019905965
cucu modification of mrna promotes decapping and transcript degradation in aspergillus eukaryotes, mrna decay is generally initiated by the shortening of the poly(a) tail mediated by the major deadenylase complex ccr4-caf1-not. the deadenylated transcript is then rapidly degraded, primarily via the decapping-dependent pathway. here we report that in aspergillus nidulans both the caf1 and ccr4 orthologues are functionally distinct deadenylases in vivo: caf1 is required for the regulated degradation of specific transcripts, and ccr4 is responsible for basal degradation. intriguin ...201019901075
a key role for vesicles in fungal secondary metabolism.eukaryotes have evolved highly conserved vesicle transport machinery to deliver proteins to the vacuole. in this study we show that the filamentous fungus aspergillus parasiticus employs this delivery system to perform new cellular functions, the synthesis, compartmentalization, and export of aflatoxin; this secondary metabolite is one of the most potent naturally occurring carcinogens known. here we show that a highly pure vesicle-vacuole fraction isolated from a. parasiticus under aflatoxin-in ...200919889978
the antifungal protein paf interferes with pkc/mpk and camp/pka signalling of aspergillus nidulans.the penicillium chrysogenum antifungal protein paf inhibits polar growth and induces apoptosis in aspergillus nidulans. we report here that two signalling cascades are implicated in its antifungal activity. paf activates the camp/protein kinase a (pka) signalling cascade. a pkaa deletion mutant exhibited reduced sensitivity towards paf. this was substantiated by the use of pharmacological modulators: paf aggravated the effect of the activator 8-br-camp and partially relieved the repressive activ ...201019889092
aspergillus fumigatus meda governs adherence, host cell interactions and medically important fungi, regulatory elements that control development and asexual reproduction often govern the expression of virulence traits. we therefore cloned the aspergillus fumigatus developmental modifier meda and characterized its role in conidiation, host cell interactions and virulence. as in the model organism aspergillus nidulans, disruption of meda in a. fumigatus dramatically reduced conidiation. however, the conidiophore morphology was markedly different between the two spec ...201019889083
heterologous expression of an agaricus meleagris pyranose dehydrogenase-encoding gene in aspergillus spp. and characterization of the recombinant enzyme.pyranose dehydrogenase (pdh) is a flavin-dependant sugar oxidoreductase found in the family agaricaceae, basidiomycetes that degrade lignocellulose-rich forest litter, and is catalytically related to the fungal enzymes pyranose 2-oxidase and cellobiose dehydrogenase. it has broad substrate specificity and displays similar activities with most sugar constituents of lignocellulose including disaccharides and oligosaccharides, a number of (substituted) quinones, and metal ions are suitable electron ...201019888575
genome mining of mycosporine-like amino acid (maa) synthesizing and non-synthesizing cyanobacteria: a bioinformatics study.mycosporine-like amino acids (maas) are a family of more than 20 compounds having absorption maxima between 310 and 362 nm. these compounds are well known for their uv-absorbing/screening role in various organisms and seem to have evolutionary significance. in the present investigation we tested four cyanobacteria, e.g., anabaena variabilis pcc 7937, anabaena sp. pcc 7120, synechocystis sp. pcc 6803 and synechococcus sp. pcc 6301, for their ability to synthesize maa and conducted genomic and phy ...201019879348
ppoc from aspergillus nidulans is a fusion protein with only one active aspergillus nidulans ppos [psi (precocious sexual inducer)-producing oxygenases] are required for the production of so-called psi factors, compounds that control the balance between the sexual and asexual life cycle of the fungus. the genome of a. nidulans harbours three different ppo genes: ppoa, ppob and ppoc. for all three enzymes two different haem-containing domains are predicted: a fatty acid haem peroxidase/dioxygenase domain in the n-terminal region and a p450 haem-thiolate domain in ...201019878096
aspects on evolution of fungal beta-lactam biosynthesis gene clusters and recruitment of trans-acting factors.penicillins and cephalosporins are beta-lactam antibiotics. the formation of hydrophobic penicillins has been reported in fungi only, notably penicillium chrysogenum and aspergillus (emericella) nidulans, whereas the hydrophilic cephalosporins are produced by both fungi, e.g., acremonium chrysogenum (cephalosporin c), and bacteria. the producing bacteria include gram-negatives and gram-positives, e.g., streptomyces clavuligerus (cephamycin c) and lysobacter lactamgenus (cephabacins), respectivel ...200919863978
in vivo analysis of the functions of gamma-tubulin-complex enhance our understanding of the function(s) of gamma-tubulin-complex proteins (gcps), we identified and analyzed the functions of the aspergillus nidulans homologs of gcp2-gcp6 (here designated gcpb-gcbf). the gamma-tubulin small complex (gamma-tusc) components, gamma-tubulin, gcpb and gcpc, are essential for viability and mitotic spindle formation, whereas gcpd-gcpf are not essential for viability, spindle formation or sexual reproduction. gcpd-gcpf function in reducing the frequency of chr ...200919861490
peroxisomal localization and function of nadp+ -specific isocitrate dehydrogenases in yeast.yeast peroxisomal nadp(+)-specific isocitrate dehydrogenase (idp3) contains a canonical type i peroxisomal targeting sequence (a carboxyl-terminal cys-lys-leu tripeptide), and provides the nadph required for beta-oxidation of some fatty acids in that organelle. cytosolic yeast idp2 carrying a pts1 (idp2(+ckl)) was only partially localized to peroxisomes, and the enzyme was able to function in lieu of either peroxisomal idp3 or cytosolic idp2. the analogous isocitrate dehydrogenase enzyme (idpa) ...201019854152
cloning of aspergillus nidulans dna. i. selection and analysis of recombinant plasmids capable of complementing pyrf, argif and proab mutations in escherichia coli.fragments of aspergillus nidulans dna obtained after digestion with ecori, bamhi and hindiii endonucleases were cloned in escherichia coli in plasmid pbr322. these gene banks were used for transformation of 15 e. coli auxotrophic mutants and in 5 cases prototrophic clones containing recombinant plasmids were selected. three different recombinant plasmids conferring prototrophy to pyrf, proab and argif mutants were analyzed. hybridization experiments indicated that in two of these plasmids the in ...198019852107
a fungal ph-responsive signaling pathway regulating aspergillus adaptation and invasion into the investigate the role of palb and pacc, two components of a ph-responsive signal-transduction pathway of aspergillus nidulans, during the pathogenesis of fungal infection of the cornea.201019850840
genetic analysis of conidiation regulatory pathways in koji-mold aspergillus oryzae.conidia of koji-mold aspergillus oryzae are often used as starters in the fermented food industry. however, little is known about conidiation regulation in a. oryzae. to improve the productivity of conidia in a. oryzae, it is necessary to understand conidiation regulation in the strain. therefore, we analyzed the conidiation regulatory system in a. oryzae using 10 kinds of conidiation regulatory gene disruptants. the phenotypes of aorflug, aorflba, aorflbb, aorflbc, aorflbd, aorflbe, aorbrla, ao ...201019850144
dynein light intermediate chain in aspergillus nidulans is essential for the interaction between heavy and intermediate chains.cytoplasmic dynein is a complex containing heavy chains (hcs), intermediate chains (ics), light intermediate chains (lics), and light chains (lcs). the hcs are responsible for motor activity. the ics at the tail region of the motor interact with dynactin, which is essential for dynein function. however, functions of other subunits and how they contribute to the assembly of the core complex are not clearly defined. here, we analyzed in the filamentous fungus aspergillus nidulans functions of the ...200919837669
reconstitution of protection against aspergillus infection in chronic granulomatous disease (cgd). 200919833851
remediation of arsenic in soil by aspergillus nidulans isolated from an arsenic-contaminated site.high concentrations of heavy metals, such as arsenic, in soils have potential long-term environmental and health consequences due to their persistence in the environment and their associated toxicity to biological organisms. aspergillus nidulans isolated from arsenic-contaminated soil has the potential to remove arsenic from soil. the isolated resistant strain showed resistance up to 500 ppm and the mean weight was found to be 1.309 g. the main objective of this research was to study the improve ...200919803330
bicarbonate gradients modulate growth and colony morphology in aspergillus nidulans.fungal colonies expand through apically extending peripheral hyphae. these long and relatively unbranched leaders leave behind cells that show a higher degree of branching and lower growth rates. towards the centre of the colony, quiescent, but viable cells sustain processes such as transport of water, solutes and organelles, and support the concerted genesis of specialized reproductive structures. a survey of chemical signals controlling changes in hyphal growth and branching of aspergillus nid ...200919799635
recombinogenic activity of fluoxetine in aspergillus nidulans.the recombinogenic potential of fluoxetine, an antidepressant widely prescribed in the treatment of depressive disorders in cancer patients, was investigated in this study. a heterozygous diploid strain of aspergillus nidulans was utilized. fluoxetine at 7.5, 15, and 30 microm concentrations induced homozygosity of several nutritional genetic markers and significantly increased their homozygotization index values. since mitotic recombination is a mechanism leading to malignant growth through the ...200919793026
uncovering transcriptional regulation of glycerol metabolism in aspergilli through genome-wide gene expression data analysis.glycerol is catabolized by a wide range of microorganisms including aspergillus species. to identify the transcriptional regulation of glycerol metabolism in aspergillus, we analyzed data from triplicate batch fermentations of three different aspergilli (aspergillus nidulans, aspergillus oryzae and aspergillus niger) with glucose and glycerol as carbon sources. protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having ...200919784673
the aspergillus genome database, a curated comparative genomics resource for gene, protein and sequence information for the aspergillus research community.the aspergillus genome database (aspgd) is an online genomics resource for researchers studying the genetics and molecular biology of the aspergilli. aspgd combines high-quality manual curation of the experimental scientific literature examining the genetics and molecular biology of aspergilli, cutting-edge comparative genomics approaches to iteratively refine and improve structural gene annotations across multiple aspergillus species, and web-based research tools for accessing and exploring the ...201019773420
cloning of a heat-stable chitin deacetylase gene from aspergillus nidulans and its functional expression in escherichia coli.a gene encoding chitin deacetylase was cloned by polymerase chain reaction from aspergillus nidulans. sequencing result showed 40% homology to the corresponding gene from colletotrichum lindemuthianum. the complete gene contains an open reading frame of 747 nucleotides encoding a sequence of 249 amino acid residues. the chitin deacetylase gene was subcloned into a pet28a expression vector and expressed in escherichia coli bl21 and then purified by metal affinity chromatography using a his-bind c ...201019760058
study of codon bias perspective of fungal xylanase gene by multivariate analysis.fungal xylanases has important applications in food, baking, pulp and paper industries in addition to various other industries. xylanases are produced extensively by both bacterial and fungal sources and has tremendous potential of being active at extremes of temperature and ph. in the present study an effort has been made to explore the codon bias perspective of this potential enzyme using bioinformatics tools. multivariate analysis has been used as a tool to study codon bias perspectives of xy ...200919759864
first case of extensive spinal cord infection with aspergillus nidulans in a child with chronic granulomatous disease.chronic granulomatous disease (cgd) is characterized by a defect in phagocytic cells that lead to recurrent bacterial and fungal infections. the etiology of most common fungal infections in cgd are aspergillus species. aspergillus nidulans is one of several species of aspergillus with low pathogenicity. however, it was reported to cause fatal invasive aspergillosis in patients with cgd. here we report the first cured invasive aspergillus nidulans infection with extensive involvement of the spina ...200919759498
negative roles of a novel nitrogen metabolite repression-related gene, tar1, in laccase production and nitrate utilization by the basidiomycete cryptococcus neoformans.the multicopper oxidase laccase is widespread in fungi and has great industrial importance. one puzzle regarding laccase production in the basidiomycetous yeast cryptococcus neoformans is that it is inhibited by high temperature (e.g., 37 degrees c). in this paper, we report identification of a nitrogen metabolite repression-related gene, tar1, which is responsible for laccase repression. disruption of tar1 results in a significant increase in the level of lac1 mrna at 37 degrees c. the putative ...200919734333
kapi, a non-essential member of the pse1p/imp5 karyopherin family, controls colonial and asexual development in aspergillus nidulans.asexual development in the filamentous fungus aspergillus nidulans is governed by the timely expression and cellular localization of multiple transcription factors. hence, factors mediating import and export across the nuclear pore complexes (karyopherins) are expected to play a key role in coordinating the developmental programme. here we characterize kapi, a putative homologue of the saccharomyces cerevisiae kap121/pse1p karyopherin. kapi is a non-essential importin-beta-like protein located i ...200919729403
arfb links protein lipidation and endocytosis to polarized growth of aspergillus nidulans.aspergillus nidulans undergoes polarized hyphal growth during the majority of its life cycle. regulatory mechanisms for hyphal polarity have been intensively investigated in a variety of filamentous fungi. two important cellular processes, which have received recent attention, include protein myristoylation and endocytosis. it is clear that protein myristoylation is essential for polarity establishment because germinating a. nidulans conidia lost polarity in the presence of cerulenin, a lipid me ...200819704790
the transcription factor homolog ctf1 regulates {beta}-oxidation in candida albicans.carbon starvation is one of the many stresses to which microbial pathogens are subjected while in the host. pathways necessary for the utilization of alternative carbon sources, such as gluconeogenesis, the glyoxylate cycle, and beta-oxidation of fatty acids, have been shown to be required for full virulence in several systems, including the fungal pathogen candida albicans. we have investigated the regulatory network governing alternative carbon metabolism in this organism through characterizat ...200919700635
transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress.the lysine biosynthetic pathway has to supply large amounts of alpha-aminoadipic acid for penicillin biosynthesis in penicillium chrysogenum. in this study, we have characterized the p. chrysogenum l2 mutant, a lysine auxotroph that shows highly increased expression of several lysine biosynthesis genes (lys1, lys2, lys3, lys7). the l2 mutant was found to be deficient in homoaconitase activity since it was complemented by the aspergillus nidulans lysf gene. we have cloned a gene (named lys3) that ...200919696106
organization and dynamics of the aspergillus nidulans golgi during apical extension and mitosis.aspergillus nidulans hyphae grow exclusively by apical extension. golgi equivalents (ges) labeled with mrfp-tagged ph(osbp) domain form a markedly polarized, dynamic network of ring-shaped and fenestrated cisternae that remains intact during "closed" mitosis. mrfp-ph(osbp) ges advance associated with the growing apex where secretion predominates but do not undergo long-distance movement toward the tip that could account for their polarization. mrfp-ph(osbp) ges overlap with the trans-golgi resid ...200919692566
patterns of susceptibility of aspergillus isolates recovered from patients enrolled in the transplant-associated infection surveillance network.we analyzed antifungal susceptibilities of 274 clinical aspergillus isolates from transplant recipients with proven or probable invasive aspergillosis collected as part of the transplant-associated infection surveillance network (transnet) and examined the relationship between mic and mortality at 6 or 12 weeks. antifungal susceptibility testing was performed by the clinical and laboratory standards institute (clsi) m38-a2 broth dilution method for amphotericin b (amb), itraconazole (itr), voric ...200919692558
heterologous production of non-ribosomal peptide lld-acv in saccharomyces cerevisiae.non-ribosomal peptides (nrps) are a diverse family of secondary metabolites with a broad range of biological activities. we started to develop an eukaryotic microbial platform based on the yeast saccharomyces cerevisiae for heterologous production of nrps using delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine (acv) as a model nrp. the penicillium chrysogenum gene pcbab encoding acv synthetase was expressed in s. cerevisiae from a high-copy plasmid together with phosphopantetheinyl transferase (p ...200919686863
aspergillus nidulans genes encoding reverse transsulfuration enzymes belong to homocysteine regulon.homocysteine is an intermediate in methionine synthesis in aspergillus nidulans, but it can also be converted to cysteine by the reverse transsulfuration pathway involving cystathionine beta-synthase (cbs) and cystathionine gamma-lyase (cgl). because homocysteine is toxic to the cell at high concentrations, this pathway also functions as a means of removal of its excess. we found that the transcription of the meca and mecb genes encoding cbs and cgl was upregulated by excess of homocysteine as w ...200919685245
modelling and mutational evidence identify the substrate binding site and functional elements in apc amino acid transporters.the amino acid-polyamine-organocation (apc) superfamily is the main family of amino acid transporters found in all domains of life and one of the largest families of secondary transporters. here, using a sensitive homology threading approach and modelling we show that the predicted structure of apc members is extremely similar to the crystal structures of several prokaryotic transporters belonging to evolutionary distinct protein families with different substrate specificities. all of these prot ...200919670073
aspergillus nidulans protein o-mannosyltransferases play roles in cell wall integrity and developmental patterning.protein o-mannosyltransferases (pmts) initiate o-mannosyl glycan biosynthesis from ser and thr residues of target proteins. fungal pmts are divided into three subfamilies, pmt1, -2, and -4. aspergillus nidulans possesses a single representative of each pmt subfamily, pmta (subfamily 2), pmtb (subfamily 1), and pmtc (subfamily 4). in this work, we show that single deltapmt mutants are viable and have unique phenotypes and that the deltapmta deltapmtb double mutant is the only viable double mutant ...200919666781
intimate bacterial-fungal interaction triggers biosynthesis of archetypal polyketides in aspergillus nidulans.fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. however, mining the full-genome sequences of fungi indicates that their potential to produce secondary metabolites is greatly underestimated. because most of the biosynthesis gene clusters are silent under laboratory conditions, one of the major challenges is to understand the physiological conditions under which these genes are activated. thus, we cocultivated the important model fungus aspe ...200919666480
expressing a fusion protein with protease and chitinase activities increases the virulence of the insect pathogen beauveria bassiana.entomopathogenic fungi, such as beauveria bassiana and metarhizium anisopliae are being developed as alternatives to chemical insecticides. they infect insects by direct penetration of the cuticle using a combination of physical pressure and extracellular hydrolytic enzymes such as proteases and chitinases. previously we found that overexpression of a subtilisin-like protease (pr1a) or a chitinase (bbchit1) resulted in increased virulence of m. anisopliae and b. bassiana, respectively. in this s ...200919666027
a fungal phylogeny based on 82 complete genomes using the composition vector method.molecular phylogenetics and phylogenomics have greatly revised and enriched the fungal systematics in the last two decades. most of the analyses have been performed by comparing single or multiple orthologous gene regions. sequence alignment has always been an essential element in tree construction. these alignment-based methods (to be called the standard methods hereafter) need independent verification in order to put the fungal tree of life (tol) on a secure footing. the ever-increasing number ...200919664262
methylcitrate cycle activation during adaptation of fusarium solani and fusarium verticillioides to propionyl-coa-generating carbon sources.propionyl-coa is an inhibitor of both primary and secondary metabolism in aspergillus species and a functional methylcitrate cycle is essential for the efficient removal of this potentially toxic metabolite. although the genomes of most sequenced fungal species appear to contain genes coding for enzymes of the methylcitrate cycle, experimental confirmation of pathway activity in filamentous fungi has only been provided for aspergillus nidulans and aspergillus fumigatus. in this study we demonstr ...200919661181
the bzip-type transcription factor flbb regulates distinct morphogenetic stages of colony formation in aspergillus nidulans.conidiophore formation in aspergillus nidulans involves a developmental programme in which vegetative hyphae give rise to an ordered succession of differentiated cells: foot cell, stalk, vesicle, metulae, phialides and conidia. the developmental transition requires factors that are expressed in vegetative hyphae that activate the expression of the main regulator of conidiation, brla. one such element is the bzip-type transcription factor flbb. we found that flbb(-) mutants show defective branchi ...200919656299
identification of novel cell wall destabilizing antifungal compounds using a conditional aspergillus nidulans protein kinase c mutant.despite the need for novel drugs to combat fungal infections, antifungal drug discovery is currently limited by both the availability of suitable drug targets and assays to screen corresponding targets. the aim of this study was to screen a library of small chemical compounds to identify cell wall inhibitors using a conditional protein kinase c (pkc)-expressing strain of aspergillus nidulans. this mutant is specifically susceptible to cell wall damaging compounds under pkc-repressive growth cond ...200919648579
protein o-mannosyltransferases b and c support hyphal development and differentiation in aspergillus nidulans.aspergillus nidulans possesses three pmt genes encoding protein o-d-mannosyltransferases (pmt). previously, we reported that pmta, a member of the pmt2 subfamily, is involved in the proper maintenance of fungal morphology and formation of conidia (t. oka, t. hamaguchi, y. sameshima, m. goto, and k. furukawa, microbiology 150:1973-1982, 2004). in the present paper, we describe the characterization of the pmta paralogues pmtb and pmtc. pmtb and pmtc were classified as members of the pmt1 and pmt4 ...200919648468
partitioning the apical domain of the arabidopsis embryo requires the bobber1 nudc domain protein.the apical domain of the embryo is partitioned into distinct regions that will give rise to the cotyledons and the shoot apical meristem. in this article, we describe a novel screen to identify arabidopsis thaliana embryo arrest mutants that are defective in this partitioning, and we describe the phenotype of one such mutant, bobber1. bobber1 mutants arrest at the globular stage of development, they express the meristem-specific shootmeristemless gene throughout the top half of the embryo, and t ...200919648297
deletion and overexpression of the aspergillus nidulans gata factor areb reveals unexpected pleiotropy.the aspergillus nidulans transcription factor area is a key regulator of nitrogen metabolic gene expression. area contains a c-terminal gata zinc finger dna-binding domain and activates expression of genes necessary for nitrogen acquisition. previous studies identified areb as a potential negative regulator of nitrogen catabolism showing similarity with penicillium chrysogenum nreb and neurospora crassa asd4. the areb gene encodes multiple products containing an n-terminal gata zinc finger and a ...200919628561
the spitzenkörper: a signalling hub for the control of fungal development?in aspergillus nidulans, asexual development culminates in the formation of a multicellular conidiophore that bears spores known as conidia. although several factors required for conidiophore formation have been characterized, the mechanisms underlying the transition from vegetative growth to development remain obscure. however, the recent characterization of two of these factors, flbb and flbe, provides important new insight. notably, these studies suggest that the spitzenkörper (i.e. apical bo ...200919627503
[two-step synthesis of the full length aspergillus niger lipase gene lipa leads to high-level expression in pichia pastoris].aspergillus niger lipases are important biocatalysis widely used in industries for food processing and pharmaceutical preparation. high-level expression recombinants can lead to cost effective lipase large scale production. full length gene synthesis is an efficient measure to enhance the expression level of the gene. in order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of dna and excessive pcr amplification, a frequ ...200919621578
post-genomic insights into the plant polysaccharide degradation potential of aspergillus nidulans and comparison to aspergillus niger and aspergillus oryzae.the plant polysaccharide degradative potential of aspergillus nidulans was analysed in detail and compared to that of aspergillus niger and aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. manual verification indicated that 28.4% of the a. nidulans orfs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method.while significant differences were found between the species in the numbers of orfs ass ...200919618505
morphology and development in aspergillus nidulans: a complex other filamentous fungi, aspergillus nidulans forms a multitude of cell types that facilitate colonization and development. the molecular basis of cellular morphogenesis in a. nidulans is not well, we summarize results obtained from detailed annotation of the a. nidulans genome sequence for genes with predicted roles in morphogenesis, with primary focus on polarized growth, calcium signaling, and development. we draw three broad conclusions from our results. first, the compo ...200919610202
biodiversity and evolution of primary carbon metabolism in aspergillus nidulans and other aspergillus spp.some of the aspergilli are reputed for their versatile and efficient catabolism of soluble carbon sources and related metabolites as well as raw polymeric materials. here, we present a detailed investigation of the genomic and evolutionary basis for this versatility, using seven aspergillus and one neosartorya genome sequences. we manually annotated about 155 genes per genome covering glycolysis, the pentose phosphate cycle, alternative routes of d-glucose metabolism, catabolism of d-galactose a ...200919610199
experimental evolution of resistance against a competing fungus in drosophila melanogaster.competition between microorganisms and arthropods has been shown to be an important ecological interaction determining animal development and spatial distribution patterns in saprophagous communities. in fruit-inhabiting drosophila, variation in insect developmental success is not only determined by species-specific effects of various noxious filamentous fungi but, as suggested by an earlier study, also by additive genetic variation in the ability to successfully withstand the negative impact of ...200919597847
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