Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| agrobacterium-mediated transformation of fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. | abstract agrobacterium tumefaciens-mediated transformation (atmt) has long been used to transfer genes to a wide variety of plants and has also served as an efficient tool for insertional mutagenesis. in this paper, we report the construction of four novel binary vectors for fungal transformation and the optimization of an atmt protocol for insertional mutagenesis, which permits an efficient genetic manipulation of fusarium oxysporum and other phytopathogenic fungi to be achieved. employing the ... | 2001 | 18944391 |
| identification of the novel penicillin biosynthesis gene aatb of aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene cluster. | the final step of penicillin biosynthesis in the filamentous fungus aspergillus nidulans is catalysed by isopenicillin n acyltransferase encoded by the aata gene. because there is no bacterial homologue, its evolutionary origin remained obscure. as shown here,disruption of aata still enabled penicillin production. genome mining led to the discovery of the aatb gene(an6775.3) which has a similar structure and expression pattern as aata. disruption of aatb resulted in a reduced penicillin titre. s ... | 2008 | 18942174 |
| mapping the interaction sites of aspergillus nidulans phytochrome fpha with the global regulator vea and the white collar protein lreb. | aspergillus nidulans senses red and blue-light and employs a phytochrome and a neurospora crassa white collar (wc) homologous system for light perception and transmits this information into developmental decisions. under light conditions it undergoes asexual development and in the dark it develops sexually. the phytochrome fpha consists of a light sensory domain and a signal output domain, consisting of a histidine kinase and a response regulator domain. previously it was shown that the phytochr ... | 2009 | 18936976 |
| aspergillus nidulans udp-galactopyranose mutase, encoded by ugma plays key roles in colony growth, hyphal morphogenesis, and conidiation. | growing resistance to current anti-fungal drugs is spurring investigation of new targets, including those in fungal wall metabolism. galactofuranose (galf) is found in the cell walls of many fungi including aspergillus fumigatus, which is currently the most prevalent opportunistic fungal pathogen in developed countries, and a. nidulans, a closely-related, tractable model system. udp-galactopyranose mutase (ugm) converts udp-galactopyranose into udp-galf prior to incorporation into the fungal wal ... | 2008 | 18935967 |
| the fungal phytochrome fpha from aspergillus nidulans. | the red light-sensing photoreceptor fpha from aspergillus nidulans is involved in the regulation of developmental processes in response to light. here we present extended biochemical and spectroscopic characterization of recombinant fpha using a synthetic gene with host-adapted codon usage. the recombinant photosensory domain fphan753 was shown to display all features of a bona fide phytochrome. it covalently binds biliverdin as chromophore and undergoes red/far-red light-inducible photoconversi ... | 2008 | 18931394 |
| copy number suppressors of the aspergillus nidulans nima1 mitotic kinase display distinctive and highly dynamic cell cycle-regulated locations. | the aspergillus nidulans nima kinase is essential for mitosis and is the founding member of the conserved nima-related kinase (nek) family of protein kinases. to gain insight into nima function, a copy number suppression screen has been completed that defines three proteins termed mcna, mcnb, and mcnc (multi-copy-number suppressor of nima1 a, b, and c). all display a distinctive and dynamic cell cycle-specific distribution. mcnc has weak similarity to saccharomyces cerevisiae def1 within a share ... | 2008 | 18931041 |
| characterization of the aspergillus niger prtt, a unique regulator of extracellular protease encoding genes. | expression of several aspergillus niger genes encoding major secreted, but not vacuolar, protease genes including the major acid protease gene pepa, was shown to be affected in the previously isolated a. niger protease mutant, ab1.13 [mattern, i.e., van noort, j.m., van den berg, p., archer, d.a., roberts, i.n., hondel, c.a.m.j.j., 1992. isolation and characterization of mutants of aspergillus niger deficient in extracellular proteases. molecular & general genetics 2, 332-336]. complementation c ... | 2008 | 18930158 |
| identification of genes differentially expressed in a strain of the mold aspergillus nidulans carrying a loss-of-function mutation in the pala gene. | to identify genes differentially expressed in a strain of the mold aspergillus nidulans carrying a loss-of-function mutation in pala, a gene in the ph-responsive signal transduction pathway, suppression subtractive hybridization was performed between rna isolated from the bia1 and bia1 pala1 strains grown under limiting inorganic phosphate at ph 5.0. we have identified several genes upregulated in the bia1 pala1 mutant strain that play important roles in mitotic fidelity, stress responses, enzym ... | 2008 | 18923548 |
| the pathogenicity of aspergillus nidulans. | 1948 | 18901060 | |
| mustard-gas mutation in aspergillus nidulans. | 1948 | 18898339 | |
| overexpression of a novel endogenous nadh kinase in aspergillus nidulans enhances growth. | the complete genome sequence of the filamentous fungi aspergillus nidulans has paved the way for fundamental research on this industrially important species. to the best of our knowledge, this is the first time a gene encoding for atp-dependent nadh kinase (atp:nadh 2'-phosphotransferase, ec 2.7.1.86) has been identified. the enzyme has a predicted molecular weight of 49 kda. we characterised the role of this nadh kinase by genomic integration of the putative gene an8837.2 under a strong constit ... | 2009 | 18840540 |
| effect of bioaugmentation and nitrogen supplementation on composting of paddy straw. | a composting experiment was conducted to evaluate the effect of a hyperlignocellulolytic fungal consortium and different nitrogen amendments on paddy straw composting in terms of changes in physicochemical and biological parameters. a fungal consortium comprising four lignocellulolytic mesophilic fungal cultures was used as inoculum for bioaugmentation of paddy straw in perforated pits. the comparative effect of farmyard manure (fym), soybean trash, poultry litter and urea on the composting proc ... | 2009 | 18839317 |
| characterization of bzip-type transcription factor atfa with reference to stress responses of conidia of aspergillus nidulans. | microorganisms growing in natural habitats are permanently confronted with a wide variety of external stresses. in fungi, including aspergillus nidulans, one of the general and ultimate strategies to survive in harsh habitats is the development of stress-tolerant conidia. in this study, we found that one of the basic-region leucine zipper transcription factors, named atfa, plays a crucial role in the acquisition of tolerance against oxidative and heat stress on conidia, but not on mycelia, altho ... | 2008 | 18838792 |
| depletion of the mobb and cota complex in aspergillus nidulans causes defects in polarity maintenance that can be suppressed by the environment stress. | polarized growth is a central feature in eukaryotes. establishment and maintenance of cell polarity are coordinated by signaling pathways. in this study, we have identified mobb is required for the regulation of cell polarity in aspergillus nidulans. depletion of mobb by alca (p) promoter repression or deletion of mobb abolished conidiation completely, and induced severe growth defects. mobb mutants showed abnormal nuclear segregation with increased number of nuclei in spores, but the formation ... | 2008 | 18832040 |
| the cypome (cytochrome p450 complement) of aspergillus nidulans. | the cytochromes p450 (cyps) are found in all biological kingdoms and genome sequencing projects continue to reveal an ever increasing number. the principle aim of this paper is to identify the complete cypome of aspergillus nidulans from the genome sequence version an.3 deposited at the broad institute, assign the appropriate cyp nomenclature and define function where possible. the completed analysis revealed a total of 111 cyp genes, 3 of which were previously unknown and 8 pseudogenes, represe ... | 2009 | 18824241 |
| purification and partial characterization of the xanthine-uric acid transporter (uapa) of aspergillus nidulans. | uapa, the uric acid-xanthine permease from the filamentous ascomycete aspergillus nidulans, is one of the most thoroughly characterized purine/h(+) transporters in eukaryotes. detailed studies have addressed its regulation of expression, at both the transcriptional and post-translational levels, in response to physiological and developmental signals. an extensive kinetic profile towards a plethora of purines and mutational analyses have established models on how uapa recognizes the purine ring a ... | 2009 | 18824106 |
| in vitro evaluation of penicillium digitatum sacc strains sensitivity to various fungicides from jordan. | this investigation is the first report in jordan, aimed for in vitro evaluation of six fungicides and their combinations, to control the post harvest green mold (penicillium digitatum) of citrus fruits. thirty one different concentrations (0.01-3000 microg ml(-1)) of each fungicide, in addition to 6 combined concentrations from each of 7 fungicide mixtures, were tested using agar well diffusion method against four fungal strains. regression analysis, one way anova and post hoc multiple compariso ... | 2008 | 18819638 |
| fe(ii)/alpha-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism. | fe(ii)/alpha-ketoglutarate-dependent hydroxylases uniformly possess a double-stranded beta-helix fold with two conserved histidines and one carboxylate coordinating their mononuclear ferrous ions. oxidative decomposition of the alpha-keto acid is proposed to generate a ferryl-oxo intermediate capable of hydroxylating unactivated carbon atoms in a myriad of substrates. this perspective focuses on a subgroup of these enzymes that are involved in pyrimidine salvage, purine decomposition, nucleoside ... | 2008 | 18813363 |
| a survey of nonribosomal peptide synthetase (nrps) genes in aspergillus nidulans. | the genome of aspergillus nidulans carries 27 genes encoding nonribosomal peptide synthetase (nrps) structures, although only five of these forming peptides and amino acid containing metabolites have been identified so far. this manuscript describes domain structures, substrate binding pockets and related genes and gene clusters and summarizes our current state of product prediction of fungal nrps systems. | 2009 | 18804170 |
| genotoxicity of achillea millefolium essential oil in diploid cells of aspergillus nidulans. | the essential oil of achillea millefolium is commonly used in folk medicine for the treatment of several diseases and has been demonstrated previously to exert an in vitro antimicrobial activity against human pathogens. current study investigates the genotoxic activity of a. millefolium oil. the oil's major constituents are: chamazulene (42.15%), sabinene (19.72%), terpin-4-ol (5.22%), beta-caryophyllene (4.44%) and eucalyptol (3.10%), comprising 74.63% of the total. the oil's genotoxic evaluati ... | 2009 | 18803228 |
| characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of aspergillus nidulans. | under carbon starvation, aspergillus nidulans produced a fungal/bacterial type chitinase, chib. the chib gene was cloned and subcloned into pjc40 expression vector containing a 10xhis fusion tag, and the chib protein was expressed heterologously in escherichia coli. recombinant and native chib enzymes shared the same optimal ph ranges and showed similar substrate specificities with endo-acting cleavage patterns. | 2008 | 18800599 |
| genomics of industrial aspergilli and comparison with toxigenic relatives. | aspergillus oryzae has been used in japanese fermentation industries for more than a thousand years. the species produces large amounts of various hydrolytic enzymes and has been successfully applied to modern biotechnology. the size of the a. oryzae genome (37.5 mb) is very close to that of a. flavus and a. niger, and 20-30% larger than that of either a. nidulans or a. fumigatus. a. oryzae and a. flavus have exactly the same number of aspartic proteinase genes, of which each orthologous pair sh ... | 2008 | 18798040 |
| invasive aspergillus nidulans infection in a patient with chronic granulomatous disease. | 2008 | 18783555 | |
| the level of sulfane sulfur in the fungus aspergillus nidulans wild type and mutant strains. | the interdependence of the sulfane sulfur metabolism and sulfur amino acid metabolism was studied in the fungus aspergillus nidulans wild type strain and in mutants impaired in genes encoding enzymes involved in the synthesis of cysteine (a precursor of sulfane sulfur) or in regulatory genes of the sulfur metabolite repression system. it was found that a low concentration of cellular cysteine leads to elevation of two sulfane sulfurtransferases, rhodanase and cystathionine gamma-lyase, while the ... | 2009 | 18781374 |
| an evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation. | transcriptome analysis of aspergillus niger transfer cultures grown on galacturonic acid media identified a highly correlating cluster of four strongly induced hypothetical genes linked with a subset set of genes encoding pectin degrading enzymes. three of the encoded hypothetical proteins now designated gaaa to gaac are directly involved in further galacturonic acid catabolism. functional and biochemical analysis revealed that gaaa is a novel d-galacturonic acid reductase. two non-allelic asper ... | 2008 | 18768163 |
| gdp-mannose pyrophosphorylase is essential for cell wall integrity, morphogenesis and viability of aspergillus fumigatus. | gdp-mannose pyrophosphorylase (gmpp) catalyses the synthesis of gdp-mannose, which is the precursor for the mannose residues in glycoconjugates, using mannose 1-phosphate and gtp as substrates. repression of gmpp in yeast leads to phenotypes including cell lysis, defective cell wall, and failure of polarized growth and cell separation. although several gmpps have been isolated and characterized in filamentous fungi, the physiological consequences of their actions are not clear. in this study, af ... | 2008 | 18757806 |
| the asexual pathogen aspergillus fumigatus expresses functional determinants of aspergillus nidulans sexual development. | the major fungal pathogen of humans, aspergillus fumigatus, lacks a defined sexual cycle, although the presence of genes encoding putative mating type idiomorphs and regulators of aspergillus sexual development heightens the potential for cryptic sexuality in this deuteromycete. to test the functionality of these genetic determinants, we transferred the alpha box-encoding mat1-1 idiomorph from an a. fumigatus isolate to the homothallic fertile species aspergillus nidulans. abundant formation of ... | 2008 | 18757566 |
| inducible rna interference of brlabeta in aspergillus nidulans. | an inducible rna interference (rnai) construct composed of inverted repeating alca promoters flanking the developmental regulatory gene brlabeta was tested in aspergillus nidulans. on inducing medium, the rnai strains failed to sporulate and lacked brlaalpha and brlabeta expression. rnai was specific for brlabeta, but not brlaalpha, silencing, indicating brlaalpha regulation by brlabeta. | 2008 | 18757565 |
| elusive origins of the extra genes in aspergillus oryzae. | the genome sequence of aspergillus oryzae revealed unexpectedly that this species has approximately 20% more genes than its congeneric species a. nidulans and a. fumigatus. where did these extra genes come from? here, we evaluate several possible causes of the elevated gene number. many gene families are expanded in a. oryzae relative to a. nidulans and a. fumigatus, but we find no evidence of ancient whole-genome duplication or other segmental duplications, either in a. oryzae or in the common ... | 2008 | 18725939 |
| specific interdomain synergy in the uapa transporter determines its unique specificity for uric acid among nat carriers. | uapa, a uric acid-xanthine permease of aspergillus nidulans, has been used as a prototype to study structure-function relationships in the ubiquitous nucleobase-ascorbate transporter (nat) family. using novel genetic screens, rational mutational design, chimeric nat molecules, and extensive transport kinetic analyses, we show that dynamic synergy between three distinct domains, transmembrane segment (tms)1, the tms8-9 loop, and tms12, defines the function and specificity of uapa. the tms8-9 loop ... | 2008 | 18718842 |
| evolutionary history of the ancient cutinase family in five filamentous ascomycetes reveals differential gene duplications and losses and in magnaporthe grisea shows evidence of sub- and neo-functionalization. | * the cuticle is the first barrier for fungi that parasitize plants systematically or opportunistically. here, the evolutionary history is reported of the multimembered cutinase families of the plant pathogenic ascomycetes magnaporthe grisea, fusarium graminearum and botrytis cinerea and the saprotrophic ascomycetes aspergillus nidulans and neurospora crassa. * molecular taxonomy of all fungal cutinases demonstrates a clear division into two ancient subfamilies. no evidence was found for lateral ... | 2008 | 18713314 |
| ctf1, a transcriptional activator of cutinase and lipase genes in fusarium oxysporum is dispensable for virulence. | cutinolytic enzymes are secreted by fungal pathogens attacking the aerial parts of the plant, to facilitate penetration of the outermost cuticular barrier of the host. the role of cutinases in soil-borne root pathogens has not been studied thus far. here we report the characterization of the zinc finger transcription factor ctf1 from the vascular wilt fungus fusarium oxysporum, a functional orthologue of ctf1alpha that controls expression of cutinase genes and virulence in the pea stem pathogen ... | 2008 | 18705871 |
| annotation of stress-response proteins in the aspergilli. | stress-response proteins of aspergillus nidulans, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus oryzae, aspergillus terreus and neosartorya fischeri (3908 in total) were annotated and grouped according to stress types (http://193.6.155.82/aspergillusstress/). all genomes harboured elements of the sska-hoga/saka stress signalling pathway. there are accumulating data pointing at the importance of sska-hoga/saka signalling in different types of stre ... | 2009 | 18703157 |
| purification, characterization and crystallization of an extracellular alkaline protease from aspergillus nidulans ha-10. | aspergillus nidulans is a highly potent fungus used in the production of alkaline protease. extracellular alkaline protease was purified from a. nidulans in a two-step procedure involving ammonium sulphate precipitation and sephadex g-100 column chromatography. the molecular mass of the enzyme was determined to be 42 kda by sds-page. the enzyme activity was also analyzed by zymogram with gelatin. the enzyme was more stable over a wide range of ph (6-10) and the temperatures up to 50 degrees c. i ... | 2008 | 18702069 |
| recombinant bacterial hemoglobin alters metabolism of aspergillus niger. | the filamentous fungus aspergillus niger is used extensively for the production of enzymes and organic acids. a major problem in industrial fermentations with this fungus is to ensure sufficient supply of oxygen required for respiratory metabolism of the fungus. in case of oxygen limitation, the fungus will produce various by-products like organic acids and polyols. in order to circumvent this problem we here study the effects of the expression of a bacterial hemoglobin protein on the metabolism ... | 2009 | 18694843 |
| proteins involved in microbody biogenesis and degradation in aspergillus nidulans. | fungal microbodies (peroxisomes) are inducible organelles that proliferate in response to nutritional cues. proteins involved in peroxisome biogenesis/proliferation are designated peroxins and are encoded by pex genes. an autophagy-related process, termed pexophagy, is responsible for the selective removal of peroxisomes from the cell. several genes involved in pexophagy are also required for autophagy and are collectively known as atg genes. we have re-analysed the aspergillus nidulans genome f ... | 2009 | 18694841 |
| a single channel for nitrate uptake, nitrite export and nitrite uptake by escherichia coli naru and a role for nirc in nitrite export and uptake. | two related polytopic membrane proteins of the major facilitator family, nark and naru, catalyse nitrate uptake, nitrite export and nitrite uptake across the escherichia coli cytoplasmic membrane by an unknown mechanism. a 12-helix model of naru was constructed based upon six alkaline phosphatase and beta-galactosidase fusions to nark and the predicted hydropathy for the nark family. fifteen residues conserved in the nark-naru protein family were substituted by site-directed mutagenesis, includi ... | 2009 | 18691156 |
| regulation of apical dominance in aspergillus nidulans hyphae by reactive oxygen species. | in fungal hyphae, apical dominance refers to the suppression of secondary polarity axes in the general vicinity of a growing hyphal tip. the mechanisms underlying apical dominance remain largely undefined, although calcium signaling may play a role. here, we describe the localized accumulation of reactive oxygen species (ros) in the apical region of aspergillus nidulans hyphae. our analysis of atma (atm) and prpa (parp) mutants reveals a correlation between localized production of ros and enforc ... | 2008 | 18689883 |
| farnesol induces the transcriptional accumulation of the aspergillus nidulans apoptosis-inducing factor (aif)-like mitochondrial oxidoreductase. | farnesol (foh) is a non-sterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. these isoprenoids inhibit proliferation and induce apoptosis. it has been shown previously that foh triggers morphological features characteristic of apoptosis in the filamentous fungus aspergillus nidulans. here, we investigate which pathways are influenced through foh by examining the transcriptional profile of a. nidulans exposed to this isop ... | 2008 | 18681941 |
| the aspergillus nidulans stress response transcription factor stza is ascomycete-specific and shows species-specific polymorphisms in the c-terminal region. | orthologues of the aspergillus nidulans gene stza were identified and characterised in an additional 19 fungi. these orthologues were restricted to, and found within all the pezizomycotina subphyla of the ascomycota, for which data are available, but not the saccharomycotina or taphrinomycotina subphyla. intron analysis indicated that both intron loss and gain have occurred in this gene. the orthologous proteins demonstrate considerable size variation (between 663 and 897 amino acids); with almo ... | 2008 | 18678248 |
| dissecting colony development of neurospora crassa using mrna profiling and comparative genomics approaches. | colony development, which includes hyphal extension, branching, anastomosis, and asexual sporulation, is a fundamental aspect of the life cycle of filamentous fungi; genetic mechanisms underlying these phenomena are poorly understood. we conducted transcriptional profiling during colony development of the model filamentous fungus neurospora crassa, using 70-mer oligonucleotide microarrays. relative mrna expression levels were determined for six sections of defined age excised from a 27-h-old n. ... | 2008 | 18676954 |
| dissecting individual steps of nitrogen transcription factor cooperation in the aspergillus nidulans nitrate cluster. | summary: in the ascomycete fungus aspergillus nidulans, the transcriptional activation of nitrate assimilating genes (niia, niad) depends on the cooperativity between a general nitrogen status-sensing regulator (the gata factor area) and a pathway-specific activator (the zn-cluster regulator nira). because nitrate assimilation leads to intracellular ammonium formation, it is difficult to determine the individual contributions of nira and area in this complex activation/inactivation process. in a ... | 2008 | 18673441 |
| norsolorinic acid inhibits proliferation of t24 human bladder cancer cells by arresting the cell cycle at the g0/g1 phase and inducing a fas/membrane-bound fas ligand-mediated apoptotic pathway. | 1. norsolorinic acid, isolated from aspergillus nidulans, has been shown to have antiproliferative activity in t24 human bladder cancer cells by arresting the cell cycle at the g(0)/g(1) phase and inducing apoptosis. the aim of the present study was to investigate the antiproliferative activity of norsolorinic acid in t24 human bladder cancer cells. 2. the effects of norsolorinic acid (1, 5, 10 and 20 micromol/l) on the proliferation of t24 cells and on the distribution of cells within different ... | 2008 | 18671724 |
| [evolution of meiosis of unicellulate and multicellular eucaryotes. aromorphosis at the cellular level]. | an attempt was undertaken to apply the concept elaborated for the evolution of multicellular organisms to that of unicellular eucaryotes. the latter's meiosis was formed on the basis of combination on three intracellular processes: 1) homologous dna recombination, 2) chromosome disjunction with the assistance of mitotic apparatus, and 3) formation of "linear" chromosome elements consisting of specific proteins. mechanism of homologous chromosome recombination was inherited from the archibacteria ... | 2008 | 18669302 |
| requirement of laea for secondary metabolism and sclerotial production in aspergillus flavus. | the nuclear regulator laea has been shown to govern production of multiple secondary metabolites in aspergillus nidulans and aspergillus fumigatus. herein we examine the role of this protein in aspergillus flavus. similarly as in other aspergilli, laea had a major effect on a. flavus secondary metabolism where deltalaea and over-expression laea (oe::laea) strains yielded opposite phenotypes resulting in decreased (increased) secondary metabolite production. the two mutant strains also exhibited ... | 2008 | 18667168 |
| prophylaxis against aspergillus is not perfect: problems and perils in stem cell transplantation. | availability of newer, well tolerated anti-aspergillus drugs at a time of rising prevalence of invasive aspergillosis has sparked enthusiasm for chemoprophylaxis against aspergillus in allogeneic stem cell recipients. such a drug-oriented approach however is associated with problems. in the azole class, itraconazole has been shown to be effective, but hepatotoxicity and gastrointestinal intolerability have made the drug less attractive. posaconazole, approved for prophylaxis in allogeneic stem c ... | 2009 | 18663660 |
| the design of oligonucleotide primers for the universal amplification of the n-acetylglucosaminidase gene (nag1) in chytridiomycetes with emphasis on the anaerobic neocallimastigales. | the common feature of all chytridiomycetous fungi, aerobic as well as anaerobic, is an abundance of chitin in their cell wall. the genes coding for chitinases have therefore been widely used as phylogenetic markers in ascomycetes. as their utility for chytridiomycetes has not been determined we chose the gene encoding an enzyme involved in chitin degradation and energy metabolism, the beta-(1,4)-n-acetylglucosaminidase (nag1). primer pair nag-forward and nag-reverse was used to create pcr produc ... | 2008 | 18661293 |
| functional analysis of the endoxylanase b (xynb) promoter from penicillium purpurogenum. | in penicillium purpurogenum, the gene encoding endoxylanase b (xynb) is highly expressed by xylan and repressed by glucose at the transcriptional level. the promoter of this gene has a modular structure, with eight putative xlnr binding sites in tandem (xlnr module), and upstream from them, four putative crea binding sites (crea module). promoter fragments containing different modules were inserted into a plasmid, pan49-1, which contains a basal fungal promoter linked to a reporter gene (lacz) a ... | 2008 | 18661134 |
| uvsf rfc1, the large subunit of replication factor c in aspergillus nidulans, is essential for dna replication, functions in uv repair and is upregulated in response to mms-induced dna damage. | uvsf201 was the first highly uv-sensitive repair-defective mutation isolated in aspergillus nidulans. it showed epistasis only with postreplication repair mutations, but caused lethal interactions with many other repair-defective strains. unexpectedly, closest homology of uvsf was found to the large subunit of human dna replication factor rfc that is essential for dna replication. sequencing of the uvsf201 region identified changes at two close base pairs and the corresponding amino acids in the ... | 2008 | 18655840 |
| role of the lolp cytochrome p450 monooxygenase in loline alkaloid biosynthesis. | the insecticidal loline alkaloids, produced by neotyphodium uncinatum and related endophytes, are exo-1-aminopyrrolizidines with an ether bridge between c-2 and c-7. loline alkaloids vary in methyl, acetyl, and formyl substituents on the 1-amine, which affect their biological activity. enzymes for key loline biosynthesis steps are probably encoded by genes in the lol cluster, which is duplicated in n. uncinatum, except for a large deletion in lolp2. the role of lolp1 was investigated by its repl ... | 2008 | 18655839 |
| functional diversity of chitin synthases of aspergillus nidulans in hyphal growth, conidiophore development and septum formation. | fungal chitin synthases are classified into seven classes. recent advances in genome sequence analyses have revealed that there are more than seven chitin synthase-encoding genes in the genome of filamentous ascomycete fungi. to clarify the functional differences among these genes in the morphogenesis and hyphal tip growth, we have cloned six chitin synthase-encoding genes from aspergillus nidulans and analyzed their functions. the results obtained suggest that chitin synthases are structurally ... | 2009 | 18651309 |
| expression of a synthetic artemesia annua amorphadiene synthase in aspergillus nidulans yields altered product distribution. | a gene encoding a plant terpene cyclase, artemisia annua amorpha-4,11-diene synthase (ads), was expressed in aspergillus nidulans under control of a strong constitutive promoter, (p)gpda. the transformants produced only small amounts of amorphadiene, but much larger amounts of similar sesquiterpenes normally produced as minor by-products in planta. in contrast, expression of ads in escherichia coli produced almost exclusively amorpha-4,11-diene. these results indicate that the host environment c ... | 2008 | 18651187 |
| comparison between polyethylene glycol- and polyethylenimine-mediated transformation of aspergillus nidulans. | genetic transformation of many filamentous fungi is carried out by a protocol that utilizes polyethylene glycol (peg) and calcium ion (ca2+). this method has remained practically unchanged for more than 20 years, but the roles these molecules play are not definitively understood. to gain a better understanding, we have compared peg transformation to a protocol using polyethylenimine (pei) that is the basis for non-viral transfection in mammals and which has a well established molecular model for ... | 2008 | 18649081 |
| molecular detection and identification of aspergillus spp. from clinical samples using real-time pcr. | the definite and rapid diagnosis of invasive aspergillosis is necessary because of the high mortality caused. the objective of this study was to evaluate a real-time pcr assay to detect aspergillus spp. in clinical samples, based on the light cycler technology. specificity was assessed by using dna extracted from pathogenic and non-pathogenic bacteria/fungi from spanish collection including: two aspergillus flavus, four aspergillus fumigatus, two aspergillus nidulans, two aspergillus niger and t ... | 2009 | 18643921 |
| a new protein carrying an nmra-like domain is required for cell differentiation and development in dictyostelium discoideum. | we have isolated a dictyostelium mutant unable to induce expression of the prestalk-specific marker ecmb in monolayer assays. the disrupted gene, pada, leads to a range of phenotypic defects in growth and development. we show that pada is essential for growth, and we have generated a thermosensitive mutant allele, pada(-). at the permissive temperature, mutant cells grow poorly; they remain longer at the slug stage during development and are defective in terminal differentiation. at the restrict ... | 2008 | 18638468 |
| expression of active spinach glycolate oxidase in aspergillus nidulans. | the biocatalytic production of glyoxylic acid from glycolic acid requires two enzymes: glycolate oxidase, which catalyzes the oxidation of glycolic acid by oxygen to produce glyoxylic acid and hydrogen peroxide, and catalase, which decomposes the byproduct hydrogen peroxide. as an alternative to isolation from the leaf peroxisomes of spinach, glycolate oxidase has now been cloned and expressed in transformants of aspergillus nidulans t580 at levels ranging from 1.7 to 36 iu/g dry wt. cells. the ... | 1996 | 18626962 |
| symmetric branching model for the kinetics of mycelial growth. | a mathematical model, linking microscopic to macroscopic parameters of the kinetics of mycelial growth is presented. the model consists of two parts: (a) a microscopic description, based on the assumption that growth of a mycelium can be represented approximately by the growth of a symmetric binary tree, where the branching level (microscopic state variable) is logarithmically related to the number of tips and segments; and (b) a macroscopic description which makes use of the microscopic descrip ... | 1993 | 18609641 |
| kinetics of cell growth and heterologous glucoamylase production in recombinant aspergillus nidulans. | in the work, a study of cell growth and the regulation of heterologous glucoamylase synthesis under the control of the positively regulated alca promoter in a recombinant aspergillus nidulans is presented. we found that similar growth rates were obtained for both the host and recombinant cells when either glucose or fructose was employed as sole carbon and energy source. use of the potent inducer cyclopentanone in concentrations greater than 3 mm resulted n maximum glucoamylase concentration and ... | 1993 | 18609547 |
| aspergillus fumigatus rasa regulates asexual development and cell wall integrity. | the ras family of proteins is a large group of monomeric gtpases. members of the fungal ras family act as molecular switches that transduce signals from the outside of the cell to signaling cascades inside the cell. a. fumigatus rasa is 94% identical to the essential rasa gene of aspergillus nidulans and is the ras family member sharing the highest identity to ras homologs studied in many other fungi. in this study, we report that rasa is not essential in a. fumigatus, but its absence is associa ... | 2008 | 18606827 |
| structural analysis of the recognition of the negative regulator nmra and dna by the zinc finger from the gata-type transcription factor area. | amongst the most common protein motifs in eukaryotes are zinc fingers (zfs), which, although largely known as dna binding modules, also can have additional important regulatory roles in forming protein:protein interactions. area is a transcriptional activator central to nitrogen metabolism in aspergillus nidulans. area contains a gata-type zf that has a competing dual recognition function, binding either dna or the negative regulator nmra. we report the crystal structures of three area zf-nmra c ... | 2008 | 18602114 |
| two gdp-mannose transporters contribute to hyphal form and cell wall integrity in aspergillus nidulans. | in order to identify novel genes affecting cell wall integrity, we have generated mutant strains of the filamentous fungus aspergillus nidulans that show hypersensitivity to the chitin-binding agent calcofluor white (cfw). affected loci are designated cal loci. the phenotype of one of these alleles, cali11, also includes shortened hyphal compartments and increased density of branching in the absence of cfw, as well as reduced staining of cell walls by the lectin fitc-concanavalin a (cona), which ... | 2008 | 18599832 |
| lactose and d-galactose catabolism in the filamentous fungus aspergillus nidulans. | the disaccharide lactose is a byproduct of cheese production accumulating to amounts of 800,000 tons per year worldwide, of which 15% is used as a carbon source for various microbial fermentations. nevertheless, little is known about the regulation of its metabolism in filamentous fungi. lactose is metabolized slowly, and some important fungi such as a. niger cannot use it at all. a more detailed knowledge on the rate-limiting steps would be helpful to improve its industrial application. we have ... | 2008 | 18595317 |
| heterotrimeric g protein mediated regulation of proteinase production in aspergillus nidulans. | extracellular proteinase production induced by carbon starvation was studied in a series of heterotrimeric g protein signaling pathway mutants of aspergillus nidulans. all the mutants tested--including deltafada (galpha), deltasfad (gbeta), deltagpga (ggamma) and deltasfga (regulator of fada signaling)--showed an elevated proteinase production after glucose depletion. our results strongly support the view that during growth, fada/sfad/gpga g protein signaling inhibits proteinase production via b ... | 2008 | 18595316 |
| [progress in research on apoptosis in filamentous fungi--a review]. | apoptosis is a conserved mechanism that plays an essential role in eukaryotes. malfunction of apoptosis contributes to many human diseases including cancer and aids. compared with yeast, a monocellular eukaryote, which is the model organism for apoptosis research, investigation on filamentous fungi apoptosis has particular advantages, which remained unrecognized until recent years. filamentous fungi, especially aspergillus nidulans and aspergillus fumigatus, are expected to become new model spec ... | 2008 | 18590245 |
| genome mining of cyanide-degrading nitrilases from filamentous fungi. | a variety of fungal species are known to degrade cyanide through the action of cyanide hydratases, a specialized subset of nitrilases which hydrolyze cyanide to formamide. in this paper, we report on two previously unknown and uncharacterized cyanide hydratases from neurospora crassa and aspergillus nidulans. recombinant forms of four cyanide hydratases from n. crassa, a. nidulans, gibberella zeae, and gloeocercospora sorghi were prepared after their genes were cloned with n-terminal hexahistidi ... | 2008 | 18587571 |
| pichia expression and mutagenesis of 7,8-linoleate diol synthase change the dioxygenase and hydroperoxide isomerase. | linoleate diol synthases (lds) are homologous 8(r)-dioxygenases with hydroperoxide isomerase activities, expressed in fungal pathogens of humanitarian importance. we report for the first time expression and site-directed mutagenesis of lds. 7,8-lds of the take-all fungus, expressed in pichia pastoris, oxygenated 18:2n-6 to 8(r)-hydroperoxylinoleic acid, which was unexpectedly isomerized to 5,8(r)-dihydroxylinoleic acid (60% 5s) and to 8(r),13-dihydroxyoctadeca-9(e),11(e)-dienoic acid. the latter ... | 2008 | 18586008 |
| a cytosine methyltransferase homologue is essential for sexual development in aspergillus nidulans. | the genome defense processes rip (repeat-induced point mutation) in the filamentous fungus neurospora crassa, and mip (methylation induced premeiotically) in the fungus ascobolus immersus depend on proteins with dna methyltransferase (dmt) domains. nevertheless, these proteins, rid and masc1, respectively, have not been demonstrated to have dmt activity. we discovered a close homologue in aspergillus nidulans, a fungus thought to have no methylation and no genome defense system comparable to rip ... | 2008 | 18575630 |
| nadph oxidases nox-1 and nox-2 require the regulatory subunit nor-1 to control cell differentiation and growth in neurospora crassa. | we have proposed that reactive oxygen species (ros) play essential roles in cell differentiation. enzymes belonging to the nadph oxidase (nox) family produce superoxide in a regulated manner. we have identified three distinct nox subfamilies in the fungal kingdom and have shown that noxa is required for sexual cell differentiation in aspergillus nidulans. here we show that neurospora crassa nox-1 elimination results in complete female sterility, decreased asexual development, and reduction of hy ... | 2008 | 18567788 |
| induction of mitotic crossing-over in diploid strains of aspergillus nidulans using low-dose x-rays. | as a contribution towards detecting the genetic effects of low doses of genotoxic physical agents, this paper deals with the consequences of low-dose x-rays in the aspergillus nidulans genome. the irradiation doses studied were those commonly used in dental clinics (1-5 cgy). even very low doses promoted increased mitotic crossing-over frequencies in diploid strains heterozygous for several genetic markers including the ones involved in dna repair and recombination mechanisms. genetic markers of ... | 2008 | 18561380 |
| molecular genetic mining of the aspergillus secondary metabolome: discovery of the emericellamide biosynthetic pathway. | the recently sequenced genomes of several aspergillus species have revealed that these organisms have the potential to produce a surprisingly large range of natural products, many of which are currently unknown. we have found that a. nidulans produces emericellamide a, an antibiotic compound of mixed origins with polyketide and amino acid building blocks. additionally, we describe the discovery of four previously unidentified, related compounds that we designate emericellamide c-f. using recentl ... | 2008 | 18559263 |
| velb/vea/laea complex coordinates light signal with fungal development and secondary metabolism. | differentiation and secondary metabolism are correlated processes in fungi that respond to light. in aspergillus nidulans, light inhibits sexual reproduction as well as secondary metabolism. we identified the heterotrimeric velvet complex velb/vea/laea connecting light-responding developmental regulation and control of secondary metabolism. vea, which is primarily expressed in the dark, physically interacts with velb, which is expressed during sexual development. vea bridges velb to the nuclear ... | 2008 | 18556559 |
| developmental biology. sex and poison in the dark. | 2008 | 18556538 | |
| production of the polyketide 6-msa in yeast engineered for increased malonyl-coa supply. | the heterologous production of fungal polyketides was investigated using 6-methylsalicylic acid synthase (6-msas) as a model polyketide synthase and saccharomyces cerevisiae as a host. in order to improve the production of 6-msa by enhancing the supply of precursors, the promoter of the gene (acc1) encoding acetyl-coa carboxylase, which catalyzes the conversion of acetyl-coa to malonyl-coa, was replaced with a strong, constitutive promoter (tef1p) in a strain harboring two plasmids carrying the ... | 2008 | 18555717 |
| aspergillus nidulans arfb plays a role in endocytosis and polarized growth. | filamentous fungi undergo polarized growth throughout most of their life cycles. the spitzenkörper is an apical organelle composed primarily of vesicles that is unique to filamentous fungi and is likely to act as a vesicle supply center for tip growth. vesicle assembly and trafficking are therefore important for hyphal growth. adp ribosylation factors (arfs), a group of small gtpase proteins, play an important role in nucleating vesicle assembly. little is known about the role of arfs in filamen ... | 2008 | 18539885 |
| ambient ph signalling in the yeast yarrowia lipolytica involves ylrim23p/palc, which interacts with snf7p/vps32p, but does not require the long c terminus of ylrim9p/pali. | a conserved ambient ph signal transduction pathway has been evidenced in both ascomycetous yeasts and filamentous fungi, called the rim or pal pathway, respectively. however, closely related palc orthologues are found only in yarrowia lipolytica and in filamentous fungi, where the rim9p/pali factor has a much longer c-terminal tail than in other yeasts. we show here that, like aspergillus nidulans pali mutants, a ylrim9delta mutant has a less extreme phenotype than other mutants of the pathway, ... | 2008 | 18524921 |
| a single amino acid substitution in one of the lipases of aspergillus nidulans confers resistance to the antimycotic drug undecanoic acid. | a plausible approach to evaluate the inhibitory action of antifungals is through the investigation of the fungal resistance to these drugs. we describe here the molecular cloning and initial characterization of the a. nidulans lipa gene, where mutation (lipa1) conferred resistance to undecanoic acid, the most fungitoxic fatty acid in the c(7:0)-c(18:0) series. the lipa gene codes for a putative lipase with the sequence consensus gvsis and wifggg as the catalytic signature. comparison of the wild ... | 2008 | 18516670 |
| the interaction of induction, repression and starvation in the regulation of extracellular proteases in aspergillus nidulans: evidence for a role for crea in the response to carbon starvation. | in aspergillus nidulans, production of extracellular proteases in response to carbon starvation and to a lesser extent nitrogen starvation is controlled by xprg, a putative transcriptional activator. in this study the role of genes involved in carbon catabolite repression and the role of protein as an inducer of extracellular protease gene expression were examined. the addition of exogenous protein to the growth medium did not increase extracellular protease activity whether or not additional ca ... | 2008 | 18512059 |
| structural characterisation of pina ww domain and a comparison with other group iv ww domains, pin1 and ess1. | the nmr solution structure of the pina ww domain from aspergillus nidulans is presented. the backbone of the pina ww domain is composed of a triple-stranded anti-parallel beta-sheet and an alpha-helix similar to ess1 and pin1 without the alpha-helix linker. large rms deviations in loop i were observed both from the nmr structures and molecular dynamics simulation suggest that the loop i of pina ww domain is flexible and solvent accessible, thus enabling it to bind the ps/pt-p motif. the ww domai ... | 2008 | 18503784 |
| more than a repair enzyme: aspergillus nidulans photolyase-like crya is a regulator of sexual development. | cryptochromes are blue-light receptors that have presumably evolved from the dna photolyase protein family, and the genomes of many organisms contain genes for both types of molecules. both protein structures resemble each other, which suggests that light control and light protection share a common ancient origin. in the genome of the filamentous fungus aspergillus nidulans, however, only one cryptochrome/photolyase-encoding gene, termed crya, was identified. deletion of the crya gene triggers s ... | 2008 | 18495868 |
| sexual and vegetative compatibility genes in the aspergilli. | gene flow within populations can occur by sexual and/or parasexual means. analyses of experimental and in silico work are presented relevant to possible gene flow within the aspergilli. first, the discovery of mating-type (mat) genes within certain species of aspergillus is described. the implications for self-fertility, sexuality in supposedly asexual species and possible uses as phylogenetic markers are discussed. second, the results of data mining for heterokaryon incompatibility (het) and pr ... | 2007 | 18490952 |
| effect of culture temperature on the heterologous expression of pleurotus eryngii versatile peroxidase in aspergillus hosts. | production of recombinant versatile peroxidase in aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. to further this purpose, the cdna encoding a versatile peroxidase of pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alca) promoter of aspergillus nidulans. a dependence of recombinant peroxidase production on cultivation temperature was found. lowering the culture temperature from 28 to 19 degrees c enhanced the lev ... | 2009 | 18481101 |
| apparent mrna instability in aspergillus nidulans and aspergillus terreus of a heterologous cdna encoding the major capsid antigen of rotavirus. | two expression plasmids designed to produce the rotaviral vp6 protein in aspergillus nidulans and aspergillus terreus have been constructed. in one of these plasmids the inducible a. terreus gla1 glucoamylase gene promoter and gla1 signal sequence are fused to the vp6 cdna to enable induction and extracellular secretion of the final protein product; in the other, the strong, constitutive a. nidulans gpda gene promoter has been employed. a. nidulans and a. terreus transformants containing intact ... | 1999 | 18473559 |
| two zinc finger transcription factors, crza and slta, are involved in cation homoeostasis and detoxification in aspergillus nidulans. | to investigate cation adaptation and homoeostasis in aspergillus nidulans, two transcription-factor-encoding genes have been characterized. the a. nidulans orthologue crza of the saccharomyces cerevisiae crz1 gene, encoding a transcription factor mediating gene regulation by ca(2+), has been identified and deleted. the crza deletion phenotype includes extreme sensitivity to alkaline ph, ca(2+) toxicity and aberrant morphology connected with alterations of cell-wall-related phenotypes such as red ... | 2008 | 18471095 |
| the vea regulatory system and its role in morphological and chemical development in fungi. | in fungi, the velvet gene, or vea, is involved in the regulation of diverse cellular processes, including control of asexual and sexual development as well as secondary metabolism. this global regulator is conserved in numerous fungal species. interestingly, in aspergilli, where most of the studies on vea have been carried out, this gene has been described to mediate development in response to light. in recent years the knowledge of this important regulatory system has expanded through the use o ... | 2008 | 18457967 |
| ambient ph gene regulation in fungi: making connections. | many fungi grow over a wide ph range and their gene expression is tailored to the environmental ph. in aspergillus nidulans, the transcription factor pacc, an activator of genes expressed in alkaline conditions and a repressor of those expressed in acidic conditions, undergoes two processing proteolyses, the first being ph-signal dependent and the second proteasomal. signal transduction involves a 'go-between' connecting two complexes, one of which comprises two plasma membrane proteins and an a ... | 2008 | 18457952 |
| crystal structure of a new type of nadph-dependent quinone oxidoreductase (qor2) from escherichia coli. | escherichia coli qor2 [nad(p)h-dependent quinone oxidoreductase; a ytfg gene product], which catalyzes two-electron reduction of methyl-1,4-benzoquinone, is a new type of quinone-reducing enzyme with distinct primary sequence and oligomeric conformation from previously known quinone oxidoreductases. the crystal structures of native qor2 and the qor2-nadph (nicotinamide adenine dinucleotide phosphate, reduced form) complex reveal that qor2 consists of two domains (n-domain and c-domain) resemblin ... | 2008 | 18455185 |
| rapid tip-directed movement of golgi equivalents in growing aspergillus nidulans hyphae suggests a mechanism for delivery of growth-related materials. | golgi equivalents (ges) process materials in the fungal secretory pathway. despite the importance of localized secretion in fungal tip growth, ge behaviour in living hyphae has not been documented. the distribution was monitored of an aspergillus nidulans putative ge-associated protein, copa, tagged with gfp (copa-gfp). this co-localized with a golgi body/ge marker established in other systems, alpha-2,6-sialyltransferase, tagged with red fluorescent protein (st-rfp). copa-gfp and st-rfp distrib ... | 2008 | 18451063 |
| characterization of a highly active promoter, pbbgpd, in beauveria bassiana. | the promoter of glyceraldehyde-3-phosphate dehydrogenase (gpd) gene from aspergillus nidulans (pgpda) is widely used to direct expression of target genes constitutively in fungi. however, in some species, a heterogeneous promoter is found to be of low efficiency. to obtain a high-efficiency promoter for transformation of beauveria bassiana, an entomopathogenic fungus widely used as an mycoinsecticide, a glyceraldehyde-3-phosphate dehydrogenase gene (bbgpd) promoter, was cloned and characterized. ... | 2008 | 18443858 |
| the unprocessed preprotein form iatc103s of the isopenicillin n acyltransferase is transported inside peroxisomes and regulates its self-processing. | previous studies in penicillium chrysogenum and aspergillus nidulans suggested that self-processing of the isopenicillin n acyltransferase (iat) is an important differential factor in these fungi. expression of a mutant pende(c103s) gene in p. chrysogenum gave rise to an unprocessed inactive variant of iat (iat(c103s)) located inside peroxisomes, which indicates that transport of the proiat inside these organelles is not dependent on the processing state of the protein. co-expression of the pend ... | 2008 | 18439860 |
| ndel1 promotes axon regeneration via intermediate filaments. | failure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. however, the underlying mechanisms of the latter remain unclear. in this study, we have investigated the role of the mammalian homologue of aspergillus nidulans nude, ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. ndel1 was synthesized de novo and upregulated in crushed and transected sci ... | 2008 | 18431495 |
| localization and function of adp ribosylation factor a in aspergillus nidulans. | filamentous fungi undergo polarized hyphal growth throughout the majority of their life cycle. the spitzenkörper is a structure unique to filamentous fungi that participates in hyphal growth and is composed largely of vesicles. an important class of proteins involved in vesicle assembly and trafficking are the adp-ribosylation factors (arfs). in saccharomyces cerevisiae, arf1p and arf2p are involved in secretion. aspergillus nidulans arfa is a homolog of scarf1p and scarf2p with 75% of amino aci ... | 2008 | 18430001 |
| [molecular mechanism of lissencephaly--how lis1 and ndel1 regulate cytoplasmic dynein?]. | lissencephaly is a devastating neurological disorder characterized by smooth cerebral surface, thick cortex and dilated lateral ventricules due to defective neuronal migration. lis1 was identified as a mutated gene in classical lissencephaly patients, and turned out to be a beta-subunit of platelet activating factor acetylhydrolase. studies in model organisms, particularly aspergillus nidulans, as well as those in the mouse, have uncovered an evolutionarily conserved pathway that involves lis1 a ... | 2008 | 18421979 |
| aspergillus nidulans chia is a glycosylphosphatidylinositol (gpi)-anchored chitinase specifically localized at polarized growth sites. | it is believed that chitinases play important physiological roles in filamentous fungi since chitin is one of the major cell wall components in these organisms. in this paper we investigated a chitinase gene, chia, of aspergillus nidulans and found that the gene product of chia consists of a signal sequence, a region including chitinase consensus motifs, a ser/thr/pro-rich region and a glycosylphosphatidylinositol (gpi)-anchor attachment motif. phosphatidylinositol-specific phospholipase c treat ... | 2008 | 18420434 |
| crea mediates repression of the regulatory gene xlnr which controls the production of xylanolytic enzymes in aspergillus nidulans. | the aspergillus nidulans xlnr gene encodes a zn(2)cys(6) transcription activator necessary for the synthesis of the main xylanolytic enzymes, i.e. endo-xylanases x(22), x(24) and x(34), and beta-xilosidase xlnd. expression of xlnr is not sufficient for induction of genes encoding the xylanolytic complex, the presence of xylose is absolutely required. it has been established previously that the wide-domain carbon catabolite repressor crea indirectly represses xlna (encodes x(22)) and xlnb (encode ... | 2008 | 18420433 |
| functional equivalence of translation factor eif5b from candida albicans and saccharomyces cerevisiae. | eukaryotic translation initiation factor 5b (eif5b) plays a role in recognition of the aug codon in conjunction with translation factor eif2, and promotes joining of the 60s ribosomal subunit. to see whether the eif5b proteins of other organisms function in saccharomyces cerevisiae, we cloned the corresponding genes from oryza sativa, arabidopsis thaliana, aspergillus nidulans and candida albican and expressed them under the control of the galactose-inducible gal promoter in the fun12delta strai ... | 2008 | 18414002 |
| identification and evolution of fungal mitochondrial tyrosyl-trna synthetases with group i intron splicing activity. | the bifunctional neurospora crassa mitochondrial tyrosyl-trna synthetase (cyt-18 protein) both aminoacylates mitochondrial trna(tyr) and acts as a structure-stabilizing splicing cofactor for group i introns. previous studies showed that cyt-18 has distinct trna(tyr) and group i intron-binding sites, with the latter formed by three small "insertions" in the nucleotide-binding fold and other structural adaptations compared with nonsplicing bacterial tyrosyl-trna synthetases. here, analysis of geno ... | 2008 | 18413600 |
| arp11 affects dynein-dynactin interaction and is essential for dynein function in aspergillus nidulans. | the dynactin complex contains proteins including p150 that interacts with cytoplasmic dynein and an actin-related protein arp1 that forms a minifilament. proteins including arp11 and p62 locate at the pointed end of the arp1 filament, but their biochemical functions are unclear (schroer ta. dynactin. annu rev cell dev biol 2004;20:759-779). in aspergillus nidulans, loss of arp11 or p62 causes the same nuclear distribution (nud) defect displayed by dynein mutants, indicating that these pointed-en ... | 2008 | 18410488 |
| egld, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of aspergillus nidulans. | although the process of conidial germination in filamentous fungi has been extensively studied, many aspects remain to be elucidated since the asexual spore or conidium is vital in their life cycle. breakage and reformation of cell wall polymer bonds along with the maintenance of cell wall plasticity during conidia germination depend upon a range of hydrolytic enzymes whose activity is analogous to that of expansins, a highly conserved group of plant cell wall proteins with characteristic wall l ... | 2008 | 18406638 |
| analysis of aspergillus nidulans metabolism at the genome-scale. | aspergillus nidulans is a member of a diverse group of filamentous fungi, sharing many of the properties of its close relatives with significance in the fields of medicine, agriculture and industry. furthermore, a. nidulans has been a classical model organism for studies of development biology and gene regulation, and thus it has become one of the best-characterized filamentous fungi. it was the first aspergillus species to have its genome sequenced, and automated gene prediction tools predicted ... | 2008 | 18405346 |
| polarized growth in fungi--interplay between the cytoskeleton, positional markers and membrane domains. | one kind of the most extremely polarized cells in nature are the indefinitely growing hyphae of filamentous fungi. a continuous flow of secretion vesicles from the hyphal cell body to the growing hyphal tip is essential for cell wall and membrane extension. because microtubules (mt) and actin, together with their corresponding motor proteins, are involved in the process, the arrangement of the cytoskeleton is a crucial step to establish and maintain polarity. in saccharomyces cerevisiae and schi ... | 2008 | 18399939 |