PMID(sorted ascending)
reversion of non-nitrate utilizing (nia d) mutants of aspergillus nidulans. 19695796514
permeability properties of aspergillus nidulans protoplasts.the regeneration of mycelial forms from protoplasts of mutant and wild-type strains of aspergillus nidulans was followed microscopically under conditions with which the rate of regeneration depends upon a specific transport function. this method has shown that at least four uptake systems-one for divalent anions, two for purines, and one for sugars-remain functional after removal of the cell wall.19695802597
organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi.centrifugation in sucrose density gradients of partially purified extracts from six species of fungi, i.e., rhizopus stolonifer, phycomyces nitens, absidia glauca (phycomycetes), aspergillus nidulans (ascomycetes), coprinus lagopus, and ustilago maydis (basidiomycetes), indicate that the five enzymes catalyzing steps two to six in the prechorismic acid part of the polyaromatic synthetic pathway sediment together. the sedimentation coefficients for these enzymes are very similar in the six specie ...19695802608
uv-sensitive mutants increasing mitotic crossing-over in aspergillus nidulans. 19695807344
melanin production in aspergillus nidulans. 19695810076
a kinetic study of the growth of aspergillus nidulans and other fungi. 19695822157
isolation and inhibitory effects of kb cell cultures of 3'-deoxyandenosine from aspergillus nidulans (eidam) wint. 19645836540
the two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in aspergillus nidulans. 19655848711
the use of p-fluorophenylalanine with 'master strains' of aspergillus nidulans for assigning genes to linkage groups. 19655848714
phosphatase mutants in aspergillus nidulans.three mutants at three different phosphatase loci produce inactive enzymes at 35 degrees c but partially or fully active enzynmes at 25 degrees c. furthermore, a suppressor mutant (su5pala1) which restores alkaline phosphatase activity in the pala1 mtutant is an allele of palcc4, a mzutant with a simultaneous reduction in alkaline and acid phosphatase activity. these data suggest that the phosphataseproteins may be made up of two or more different polypeptide chains, and that some of the polypep ...19655852975
origins of translocations in aspergillus nidulans. 19655857597
wild-type and mutant stocks of aspergillus nidulans. 19655857598
purification of nitrate reductase and cytochrome c reductase from aspergillus nidulans. 19655866385
resistance to various inhibitors in aspergillus nidulans. 19655867759
polarized negative interference in the lys-51 region of aspergillus nidulans. 19655872613
an investigation of the suitability of the suppressors of meth 1 in aspergillus nidulans for the study of induced and spontaneous mutation. 19655878248
complementation at the adenylosuccinase locus in aspergillus nidulans. 19655882197
isolation of 6,7-dimethyl-8-ribityl lumazine from a riboflavin-less mutant of aspergillus nidulans. 19665915130
observations on the effects of a chromosome duplication in aspergillus nidulans. 19665915374
somatically unstable mutants of aspergillus nidulans. 19665925191
recessive mutants at unlinked loci which complement in diploids but not in heterokaryons of aspergillus nidulans. 19665929247
analysis of induced mutations in aspergillus nidulans. i. uv- and hno2-induced mutations. 19665929395
studies on the inhibition and mutation of aspergillus nidulans by acridines. 19665931141
the induction and repression of nitrate reductase in the fungus aspergillus nidulans. 19665940632
kinetics of fungal growth and phosphatase formation in aspergillus nidulans.dorn, g. (albert einstein college of medicine, new york, n.y.), and w. rivera. kinetics of fungal growth and phosphatase formation in aspergillus nidulans. j. bacteriol. 92:1618-1622. 1966.-a liquid culture system is described for growing aspergillus nidulans in a manner amenable to kinetic studies. changes in dry weight, protein, deoxyribonucleic acid (dna), inorganic phosphate, alkaline phosphatase, and acid phosphatase activity were followed over a 40-hr interval. in both limiting- and high-p ...19665958101
thin-layer chromatographic separation of the metabolic products of aspergillus nidulans. 19665958835
interlocus specificity for chemical mutagens in aspergillus nidulans. 19665964558
isolation of 5-amino-4-ribitylaminouracil from a riboflavineless mutant of aspergillus nidulans. 19665971789
the influence of the cytoplasm on mutation in aspergillus nidulans. 19665972693
genetical analysis of proline mutants and their suppressors in aspergillus nidulans. 19665980118
formation of 4,5-diaminouracil in a riboflavineless mutant of aspergillus nidulans. 19665987043
a pair of pteridine derivatives in a heterokaryon of two mutants of aspergillus nidulans. 19665987145
fine structure analysis of the gene for p-fluoro-phenylalanine resistance in aspergillus nidulans. 19666004989
fine analysis of mitotic intracistronic crossing-over in aspergillus nidulans. 19666004996
electron transport pathways in riboflavinless mutants of aspergillus nidulans. 19666005316
use of analogues and the substrate-sensitivity of mutants in analysis of purine uptake and breakdown in aspergillus nidulans.aspergillus mutants resistant to various purine analogues (purine, 8-azaguanine, 2-thioxanthine, and 2-thiouric acid) are defective in at least one step of purine uptake or breakdown. the properties of these mutants show that there are two uptake systems for purines, one which mediates the uptake of hypoxanthine, guanine, and adenine, and the other, xanthine and uric acid. allantoinase-less strains are sensitive to the toxic effects of allantoin accumulation. they are severely inhibited when gro ...19676025432
complementation analysis of the tryptophan pathway in aspergillus nidulans. 19676029969
enzyme analysis of the tryptophan pathway in aspergillus nidulans. 19676029970
purification of two alkaline phosphatases from aspergillus nidulans. 19676030350
the mechanism of action of proline suppressors in aspergillus nidulans. 19676034041
genetic and cytoplasmic control of undifferentiated growth in aspergillus nidulans. 19676034180
inhibition of the lysis of fungi by melanins.evidence is presented that the resistance of aspergillus nidulans hyphae to lysis by a beta-(1-->3) glucanase-chitinase mixture results from the presence of melanin in the fungal walls. the resistance of the walls to digestion was directly correlated with the melanin content of the mycelium. a melanin-less mutant of a. nidulans was highly susceptible to hydrolysis by the enzyme mixture. preincubation of a synthetic melanin with the glucanase, chitinase, and a protease, before addition of the sub ...19676035264
kinetic studies of the induction of nitrate reductase and cytochrome c reductase in the fungus aspergillus an earlier paper (cove, 1966) it was reported that the kinetics of appearance of nitrate reductase (nadph-nitrate oxidoreductase, ec on the addition of nitrate to a growing culture of aspergillus nidulans were different in certain respects from those found for many escherichia coli enzymes. when urea is used as an initial nitrogen source, a further difference is found: enzyme synthesis is no longer continuous. this interruption of synthesis does not appear to be due to synchronous ce ...19676049855
regulation of nitrate reduction in aspergillus nidulans. 19676052720
a system for the study of interlocus specificity for both forward and reverse mutation in at least eight gene loci in aspergillus nidulans. 19676054957
synchrony of nuclear replication in individual hyphae of aspergillus nidulans.the synchrony of nuclear replication in individual, multinucleate hyphae of aspergillus nidulans has been investigated. samples were taken from cultures of germinating conidiospores, and the relative frequency of hyphae containing two to eight nuclei was determined. because the conidiospores are mononucleate, complete synchrony will yield populations of hyphae containing only 2(n) nuclei, n being the number of doublings after germination. the appearance of hyphae with total numbers of nuclei oth ...19676057801
a revised map of the eight linkage groups of aspergillus nidulans. 19676061654
chromosome instability related to gene suppression in aspergillus nidulans. 19676064104
lethal mutations and balanced lethal systems in aspergillus nidulans. 19676064441
three variant introns of the same general class in the mitochondrial gene for cytochrome oxidase subunit 1 in aspergillus nidulans.the oxia gene of aspergillus nidulans, coding for cytochrome oxidase subunit 1, is shown by dna sequencing to contain three introns. an aug start codon is not present at the beginning of the sequence, suggesting that either another codon, possibly the four base codon auga, is used for initiation or there is a further short intron between the true start codon and the beginning of the recognisable coding region. the second and third introns have long open reading frames, which could code for matur ...19846092056
the mitochondrial genome of the fission yeast schizosaccharomyces pombe: highly homologous introns are inserted at the same position of the otherwise less conserved cox1 genes in schizosaccharomyces pombe and aspergillus nidulans.the dna sequence of the second intron in the mitochondrial gene for subunit 1 of cytochrome oxidase (cox1), and the 3' part of the structural gene have been determined in schizosaccharomyces pombe. comparing the presumptive amino acid sequence of the 3' regions of the cox1 genes in fungi reveals similarly large evolutionary distances between aspergillus nidulans, saccharomyces cerevisiae and s. pombe. the comparison of exon sequences also reveals a stretch of only low homology and of general siz ...19846092057
triton x-114 phase fractionation of membrane proteins of the cyanobacterium anacystis nidulans r2.the thylakoid polypeptides of the cyanobacterium anacystis nidulans r2 were analyzed by triton x-114 phase fractionation [c. bordier (1981) j. biol. chem. 256, 1604-1607, as adapted for photosynthetic membranes by t.m. bricker and l.a. sherman (1982) febs lett. 149, 197-202]. in this procedure, polypeptides with extensive hydrophobic regions (i.e., intrinsic proteins) form mixed micelles with triton x-114, and are separated from extrinsic proteins by temperature-mediated precipitation of the mix ...19846093709
genetic analysis of mutants of aspergillus nidulans blocked at an early stage of sporulation.three mutants of aspergillus nidulans, selected to have a block at an early stage of conidiation (asexual sporulation), exhibit similar pleiotropic phenotypes. each of these mutants, termed preinduction mutants, also are blocked in sexual sporulation and secrete a set of phenolic metabolites at level much higher than wild type or mutants blocked at later stages of conidiation. backcrosses of these mutants to wild type showed that the three phenotypes always cosegregated. diploids containing the ...19846094473
regulation of gene expression in aspergillus nidulans. 19846101115
[the cyclic nucleotide system of aspergillus nidulans under the influence of methylbenzimidazol-2-ylcarbamate (mbc). i. a hypothetical mitosis model].the influence of methylbenzimidazol-2-yl carbamate (mbc) on the cyclic nucleotide system of germinating aspergillus nidulans conidia has been investigated throughout the cell cycle. the content in cyclic adenosine 3',5'-monophosphate (camp) and cyclic guanosine 3',5'-monophosphate (cgmp) is rapidly decreased in the course of conidia swelling and has been found not to be influenced by mbc. on the other hand, influence of mbc leads, in inhibited nuclear division, to a significant increase of the i ...19806106987
l-histidine utilization in aspergillus nidulans.histidase activity rather than uptake of l-histidine is the limiting factor for the utilization of histidine as the sole nitrogen source for aspergillus nidulans. histidine cannot act as the sole carbon source, and evidence is presented indicating that this is attributable to an inability to convert histidine to l-glutamate in vivo. it has been shown that this fungus lacks an active urocanase enzyme and that histidine is quantitatively converted to urocanate, which accumulates in the extracellul ...19826120926
the regulation of nadp-linked isocitrate dehydrogenase in aspergillus nidulans.the regulation of nadp-linked isocitrate dehydrogenase (nadp-idh) has been studied in wild-type and mutant strains of aspergillus nidulans. in the wild-type strain studied, the levels of nadp-idh vary in a similar way to those of acetamidase, acetyl-coa synthase, isocitrate lyase and malate synthase under all growth conditions used. similarly, fac mutants, which are altered in the regulation of these enzymes of acetate utilization, are affected in nadp-idh levels in a parallel fashion, as are cr ...19826123545
regulation of alcohol dehydrogenase (adh) and aldehyde dehydrogenase (alddh) in aspergillus nidulans. 19836132393
new leucine auxotrophs of aspergillus nidulans.a series of aspergillus nidulans leucine auxotrophs were isolated after nitroquinoline-1-oxide treatment and analyzed. four complementation groups could be distinguished in addition to the previously known leua group. a putative mutant for isopropylmalate isomerase (leub) was mapped on chromosome i and found to be closely linked to leua.19806155050
the preparation and partial characterization of antigenic fractions obtained from the mycelial walls of several aspergillus species.extracts with immunological activity were prepared from aspergillus fumigatus, a. flavus, a. terreus, a. niger and a. nidulans. in each case crude mycelial wall was extracted with an aqueous solution of triton x-100 giving detergent-soluble material. further fractionation was achieved by removing the detergent from this solution; the resultant precipitate was removed by centrifugation, and the aqueous supernatant was used as a source of soluble antigens. the sensitivity of these preparations was ...19806157778
the effect of the synergy between sterigmatocystin and a fungal metabolite on bacillus subtilis. 19826175614
recombinogenic and mutagenic effects of the antitumor antibiotic bleomycin in aspergillus nidulans.bleomycin, an antibiotic and antineoplastic drug that inhibits dna synthesis and causes several types of chromosomal aberration, was found to increase mitotic recombination in aspergillus nidulans. heterozygous prototrophic diploid strains grown on media containing bleomycin produced significant increases of yellow and white sectors compared with controls. further, the increased colour segregants were due to mitotic crossing-over, whereas the non-dis junctional segregants remained at the control ...19826181398
dna sequence of the region adjacent to the oxi1 and oxi2 genes from aspergillus nidulans mitochondrial dna. 19826184922
effect of alpha-amanitin and actinomycin d on rna synthesis in the protoplasts of aspergillus nidulans.rna synthesis was studied in protoplasts from aspergillus nidulans, which remained metabolically active for several days under the appropriate conditions. it is concluded, that at certain actinomycin d concentrations the ribosomal rna synthesis can be selectively inhibited. however, alpha-amanitin at concentrations up to 400 micrograms/ml neither influenced the total nor the rna polymerase ii directed rna synthesis. it was possible to discriminate between the rna polymerase i or ii directed rna ...19826186893
development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues.we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ...19836195179
high-frequency conversion to a "fluffy" developmental phenotype in aspergillus spp. by 5-azacytidine treatment: evidence for involvement of a single nuclear gene.transient exposure of mycelia from aspergillus niger and aspergillus nidulans to the cytidine analog 5-azacytidine, leading to no more than 0.3 to 0.5% substitution for cytosine by 5-azacytosine in a. nidulans dna, resulted in the conversion of a high fraction of the cell population (more than 20%) to a mitotically and meiotically stable "fluffy" developmental phenotype. the phenotypic variants are characterized by the developmentally timed production of a profuse fluffy network of undifferentia ...19836197627
cloning of aspergillus nidulans ribosomal dna in shotgun experiments using the ek2 vector lambda gtwest.t5-622.ecori fragments of aspergillus nidulans dna were cloned in a shotgun type experiment using the ek2 vector lambda gtwest.t5-622. in situ plaque hybridization was used to screen for hybrid phage particles containing sequences coding for ribosomal rna. four clones selected from the whole gene bank were characterized further. two of them contain sequences homologous to a 3.6 kb fragment of a. nidulans rdna.19836198874
cloning and mapping of fourteen different dna fragments containing aspergillus nidulans 5s rrna genes.fourteen different plasmids hybridizing to aspergillus nidulans 5s rrna were isolated from a gene bank obtained after cloning sau3a partial digests of a. nidulans dna in a yeast--escherichia coli vector, pbb29. the restriction maps of these plasmids were determined. the size of the cloned fragments was 2.7-9.5 kb, 12 of the plasmids were found to code for single 5s rrna genes and 2 coded for 2 genes. no similarity of the sequences surrounding the 5s rrna genes was found by restriction mapping.19846205550
polyamines. 19846206782
specificity of antigens from pathogenic aspergillus species. ii. studies with line immunoelectrophoresis.somatic (mycelial) and metabolic (culture filtrate) antigens of aspergillus flavus, a. fumigatus, a. nidulans, a. niger and a. terreus were compared by line immunoelectrophoresis with sera from patients with allergic bronchopulmonary aspergillosis (abpa) or aspergilloma, or from immunized animals. number of lines observed when tested with human sera were similar for somatic and metabolic preparations of a. fumigatus, but up to 33 lines were present when both types of antigens were tested simulta ...19846209814
active rna: rna enzymes in rna splicing and processing. 19846210116
aspergillus contains multiple tubulin genes.previous work with benomyl-resistant mutants of aspergillus nidulans has demonstrated that the bena locus is a structural gene for beta-tubulin. two of the bena mutants, bena22 and bena85, show altered electrophoretic mobilities on two-dimensional gels for two beta-tubulins (designated beta 1 and beta 2). these shifts of the beta 1- and beta 2-tubulins uncover a spot in the region where wild-type beta-tubulins migrate that is occluded on gels of wild-type extracts by the beta 1- and beta 2-tubul ...19846210290
international commission for protection against environmental mutagens and carcinogens. committee 2 final report. mutagenesis testing as an approach to carcinogenesis. 19826214712
different biosynthetic pathways of the pyrimidine moiety of thiamin in procaryotes and eucaryotes.[14c]formate is incorporated into the c-2 of the pyrimidine moiety of thiamin by escherichia coli and salmonella typhimurium. in saccharomyces cerevisiae, it is incorporated into c-4. radioactive carbons of [1-14c]glycine and [2-14c]glycine are incorporated by s. typhimurium into the c-4 and c-6 of the pyrimidine, respectively, but not by s. cerevisiae. these facts suggest that procaryotes and eucaryotes have different biosynthetic pathways for pyrimidine. in this study, the procaryotes tested i ...19836218832
intron within the large rrna gene of n. crassa mitochondria: a long open reading frame and a consensus sequence possibly important in splicing.we describe the sequence of the 2295 nucleotide long intron and 245 nucleotides of the flanking exon sequences within the large (24s) rrna gene of neurospora crassa mitochondria. the intron contains a long open reading frame, which could correspond to ribosomal protein s5. comparison with the corresponding intron of the large rrna gene of yeast mitochondria reveals a single highly homologous 57 nucleotide long sequence, including the sequence (formula; see text), which is present in virtually al ...19826218884
cell wall degradation in the autolysis of filamentous fungi.a systematic study on autolysis of the cell walls of fungi has been made on neurospora crassa, botrytis cinerea, polystictus versicolor, aspergillus nidulans, schizophyllum commune, aspergillus niger, and mucor mucedo. during autolysis each fungus produces the necessary lytic enzymes for its autodegradation. from autolyzed cultures of each fungus enzymatic precipitates were obtained. the degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell ...19826219290
transformation of aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of neurospora crassa.relief of an auxotrophic requirement for uridine in aspergillus nidulans strain g191 has been achieved by transformation with a segment of neurospora crassa dna containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. the mitotic stability of such transformants suggests that the dna has integrated into the genome. southern hybridisation analysis of dna isolated from transformants revealed the presence of pbr322 sequences which have integrated into the host genome along ...19836220717
cytochrome b gene of neurospora crassa mitochondria. partial sequence and location of introns at sites different from those in saccharomyces cerevisiae and aspergillus nidulans.we have sequenced a 2614-base pair fragment of the neurospora crassa mitochondrial dna which contains part of the structural gene for apocytochrome b. this gene is split by at least two introns. the sequence reported here begins within one intron, extends through the next exon, another intron 1276 base pairs long, and the last exon which encodes the cooh terminus of cytochrome b. within the 254 amino acids encoded by the two exons, there is a high degree of sequence conservation, 81%, with cytoc ...19846231283
genetic studies in micro-organisms producing beta-lactam antibiotics. 19846237946
cellular and extracellular siderophores of aspergillus nidulans and penicillium chrysogenum.aspergillus nidulans and penicillium chrysogenum produce specific cellular siderophores in addition to the well-known siderophores of the culture medium. since this was found previously in neurospora crassa, it is probably generally true for filamentous ascomycetes. the cellular siderophore of a. nidulans is ferricrocin; that of p. chrysogenum is ferrichrome. a. nidulans also contains triacetylfusigen, a siderophore without apparent biological activity. conidia of both species lose siderophores ...19816242827
physical map of aspergillus nidulans mitochondrial genes coding for ribosomal rna: an intervening sequence in the large rrna cistron.a detailed map of the 32 kb mitochondrial genome of aspergillus nidulans has been obtained by locating the cleavage sites for restriction endonucleases pst i, bam h i, hha i, pvu ii, hpa ii and hae iii relative to the previously determined sites for eco r i, hind ii and hind iii. the genes for the small and large ribosomal subunit rnas were mapped by gel transfer hybridization of in vitro labelled rrna to restriction fragments of mitochondrial dna and its cloned eco r i fragment e3, and by elect ...19806246396
introduction of transposon tn901 into a plasmid of anacystis nidulans: preparation for cloning in cyanobacteria.we have used the tem beta-lactamase transposon tn901, located on escherichia coli plasmid pri46, to introduce in vivo a genetic marker into plasmid puh24, present in the cyanobacterial strain anacystis nidulans r-2. restriction enzyme analysis and heteroduplex studies of the 8.3 x 10(6)-dalton plasmids pch1-pch5, present in the ampicillin-resistant a. nidulans r-2 colonies obtained after transformation with pri46, demonstrated that these plasmids consist of the complete sequence of tn901 inserte ...19806246495
mitochondrial heterogeneity in aspergillus nidulans: evidence of in vivo transformation among different mitochondrial populations.the distribution of mitochondria from aspergillus nidulans on a dextran-sucrose gradient resulted in the fractionation of three bands. the lightest band presented the highest respiratory and cytochrome c oxidase activities and was also the most active in the incorporation of tritiated glycerol. pulse-chase experiments with 3h-glycerol suggested that mitochondria from the light band was transformed to denser organelles as the organism grew.19806248928
conservation and rearrangement of mitochondrial structural gene sequences.mitochondria contain the simplest dna molecules that are present in eukaryotes. mitochondrial dna (mtdna) is easily purified, and is an important model system for studying eukaryote gene structure and basic molecular processes. the protein sequences of mitochondrial gene products have been shown to be conserved from yeast to man, and there are definite similarities at the dna sequence level. in contrast, the overall organization of the mitochondrial genome is drastically different in these organ ...19806253835
mitochondrial heterogeneity in aspergillus nidulans: in vivo protein biosynthetic activities of the mitochondrial populations.two mitochondrial populations with cytochrome c oxidase activity, and densities of 1.07 and 1.08 g/ml have been separated by means of a dextran-sucrose gradient. the highest enzymatic specific activity appeared in the 1.07 g/ml band. the mitochondria assayed by their in vivo cycloheximide resistant leucine incorporation appeared at the 1.08 g/ml density band of the gradient. some amount of activity of cytochrome c oxidase, and leucine incorporation appeared at the bottom of the gradient. the cyt ...19806261314
nuclear and mitochondrial suppression of a mitochondrially inherited cold-sensitive mutation in aspergillus nidulans.partial suppressors of a mitochondrially inherited mutation, [cs-67], conferring cold-sensitivity at 20 degrees c were identified. these mapped at one mitochondrial and four unlinked nuclear loci. most suppressors partially restored the cytochrome aa3 deficiency of the cold-sensitive strain at 20 degrees c. strains carrying two or more suppressors and [cs-67] showed considerably impaired growth. this effect was temperature-dependent, being more severe at 37 degrees c, and was not expressed in th ...19806262432
the cloning of aspergillus nidulans mitochondrial dna in escherichia coli on plasmid pbr322. 19816270505
nucleotide sequence of the aspergillus nidulans mitochondrial gene coding for the small ribosomal subunit rna: homology to e. coli 16s rrna.the complete primary structure of the 1437 bp gene coding for mitochondrial 15s rrna and its flanking regions was determined by maxam-gilbert sequencing of cloned hindiii fragment h3 of a. nidulans mtdna. the gene product reveals significant homology (59%) to e. coli 16s rrna, and the potential secondary structures of both rrna molecules are very similar, except that the hairpin structures 7, 8 and 30 of the brimacombe 16s rrna model are deleted, and that two sequences of 8 and 31 nucleotides ar ...19816273804
vectors for cloning in cyanobacteria: construction and characterization of two recombinant plasmids capable of transformation of escherichia coli k12 and anacystis nidulans r2.two plasmids were constructed consisting of the e. coli vector pacyc184 and the cyanobacterial plasmid puc1. these recombinants, designated puc104 and puc105, can be transformed to e. coli k12 as well as to the cyanobacterium anacystis nidulans r2 and in both hosts they express their antibiotic markers. puc104 and puc105 differ with respect to the location and the orientation of the pacyc184 segment in puc1. puc104 was found to be stable under all circumstances. transformation of puc105 to a. ni ...19816276694
construction of a hybrid plasmid capable of replication in the bacterium escherichia coli and the cyanobacterium anacystis nidulans.a hybrid plasmid was constructed between the 5.3-megadalton plasmid (puh24) of anacystis nidulans r2 and the escherichia coli plasmid pbr322. this was accomplished by adding a transposon to pbr322 and transforming this dna into a. nidulans. one resultant hybrid, pls103, had a molecular weight of 6.8 x 10(6), replicated in both organisms, had unique sites for two restriction endonucleases, conferred ampicillin resistance on both organisms, and could be used as a cloning vector in a. nidulans.19826277870
restriction enzyme analysis of mitochondrial dna of members of the genus aspergillus as an aid in taxonomy.restriction endonuclease patterns of mitochondrial dna from seven species of the genus aspergillus (a. nidulans, a. wentii, a. awamori, a. niger, a. oryzae, a. tamarii and a. echinulatus) have been compared in order to test the phylogenetic relationships between these species. the fraction of restriction fragments common between all pairs of species from this set ranged from 0 to 1. the use of this approach for the taxonomy of the genus aspergillus is discussed. the postulated phylogenetic relat ...19826281364
organization of the ribosomal rna gene cluster in aspergillus nidulans.dna coding for ribosomal rna in aspergillus nidulans was found to consist of a unit 7.8 kb in size which is tandemly repeated in the genome and codes for 5.8s, 18s and 26s rrna. the repeat unit has been cloned, and its restriction map and the location of the individual rrna coding sequences within the unit have been established.19826282710
nucleotide sequence of a region of the mitochondrial genome of aspergillus nidulans including the gene for atpase subunit 6.a 1500 bp fragment of the aspergillus nidulans mitochondrial genome contains genes for arginine and asparagine trnas, an unassigned reading frame, and the structural gene for atpase subunit 6. the trna genes possess 66% nucleotide homology and possibly originated by a relatively recent duplication event. the unassigned reading frame displays a low level of homology with the human urf a6l. the predicted amino acid sequence of the a-nidulans atpase subunit 6 gene is 40% homologous to the yeast pol ...19826285306
cloning and expression in escherichia coli k-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, aspergillus nidulans.a 1.35 md dna hindiii fragment containing part of the arom gene cluster or cluster gene of aspergillus nidulans encoding biosynthetic dehydroquinase (5-dehydroquinate hydrolyase) has been cloned in plasmid pbr322 on the basis of functional expression in escherichia coli. the fungal fragment on pbr322, designated phk29, complements a corresponding e. coli dehydroquinase structural gene (arod) mutation. phk29 contains one bamhi, hpaii, psti, smai, xhoi and surprisingly, one hindiii site since phk2 ...19826287167
nucleotide sequence of aspergillus nidulans mitochondrial genes coding for atpase subunit 6, cytochrome oxidase subunit 3, seven unidentified proteins, four trnas and l-rrna.the complete nucleotide sequence of a 14 kb segment of a. nidulans mtdna reveals a rather compact organization of genes transcribed from the same strand and coding for two functionally known proteins, seven unidentified polypeptides (urfs), 24 trnas and two rrnas. one of the urfs is located in the intron of the l-rrna gene and codes for a basic protein of 410 residues. the other urfs are in spacer regions and code for hydrophobic proteins. urfa is homologous to human urf4, and urfb produces a po ...19826290989
proton pump coupled to cytochrome c oxidase in the cyanobacterium anacystis nidulans.intact spheroplasts of the cyanobacterium anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. in the coupled state, h+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. it is concluded that the proton translocation observed is an intrinsic property of the cytoplasmic membrane-bound cytochrome c oxidase of a. nidulans.19836294060
genetic approaches to the analysis of microbial development. 19826297378
a new approach for molecular cloning in cyanobacteria: cloning of an anacystis nidulans met gene using a tn901-induced mutant.a new strategy for molecular cloning in the cyanobacterium anacystis nidulans r-2 is described. this strategy involved the use of a transposon and was developed for the cloning of a gene encoding methionine biosynthesis. a met::tn901 mutant was isolated. chromosomal dna fragments were cloned in the escherichia coli plasmid vector pacyc184. a recombinant plasmid carrying the inactivated met::tn901 gene was selected after transformation to e. coli. the cloned met::tn901 dna fragment was used as a ...19826298064
a physical map of the ribosomal dna repeat unit of aspergillus nidulans.the ribosomal dna repeat unit of aspergillus nidulans has been cloned in pbr322 and a restriction map constructed. the genes coding for the 17s, 5.8s and 25s rrnas are found in blocks separated by a 1.7 kb spacer region, with the 5.8s rna gene lying between the genes for the two larger rnas. the total length of the repeat unit is 7.7 kb. the 5s rrna is not present in the repeat unit.19826299883
a new hybrid plasmid capable of transforming escherichia coli and anacystis nidulans.we have constructed a hybrid plasmid, pdf30, by combining the 8-kb pdf3 plasmid derived from the cyanobacterium anacystis nidulans 6311 with the escherichia coli vector pbr325. pdf30 transforms, replicates and confers chloramphenicol resistance (cmr) and ampicillin resistance (apr) on both a. nidulans and e. coli. the level of resistance to ampicillin in a. nidulans transformants, although low, is above background resistance and an apr activity was demonstrated in cell-free extracts of a. nidula ...19836307827
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