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effect of nacl on heat resistance, antibiotic susceptibility, and caco-2 cell invasion of salmonella.this study evaluated the effects of nacl on heat resistance, antibiotic susceptibility, and caco-2 cell invasion of salmonella. salmonella typhimurium nccp10812 and salmonella enteritidis nccp12243 were exposed to 0, 2, and 4% nacl and to sequential increase of nacl concentrations from 0 to 4% nacl for 24 h at 35°c. the strains were then investigated for heat resistance (60°c), antibiotic susceptibility to eight antibiotics, and caco-2 cell invasion efficiency. s. typhimurium nccp10812 showed in ...201323936782
sef14 fimbriae from salmonella enteritidis play a role in pathogenitic to cell model in vitro and host in vivo.the role of sef14 fimbriae in virulence remains to be elucidated and in this study, we showed that sefa mutant constructed in the wild-type (wt) salmonella enteritidis strain 50336 displayed increased invasion to ipec-j2 cell lines and survival in mouse peritoneal macrophages, and the lethal dose 50% (ld50) in 6-week-old balb/c mice intra-peritoneally injected with wt s. enteritidis strain decreased significantly upon deletion of sefa indicating their role in virulence. overall, these results de ...201323933167
infectious aortitis in a liver transplant recipient.the development of an abdominal aortic aneurysm secondary to infectious aortitis following solid organ transplantation is a rare event that in the absence of surgical intervention, can lead to uncontrolled sepsis, catastrophic hemorrhage and death. arterial allografts have been a viable surgical option for the past 30 years, although operative modalities have undergone a paradigm shift in recent years. we describe the first case in the literature of a liver transplant recipient who developed an ...201323919247
altered regulation of elavl1/hur in hla-b27-expressing u937 monocytic cells.to investigate the role of hla-b27 expression in the regulation of rna binding protein (rbp) embryonic lethal abnormal vision (elav) l1/human antigen r (hur) expression in salmonella-infected or lps-stimulated human monocytic cells, since hur is a critical regulator of the post-transcriptional fate of many genes (e.g. tnfα) important in inflammatory response.201323894643
mycotic aneurysm of the internal carotid artery.infected aneurysms of the extracranial carotid arteries are uncommon. this article presents a complete review of the literature on this condition, illustrated with a case report. this case report describes a mycotic aneurysm of carotid bifurcation with proven infection of the arterial wall from salmonella enteritidis. the treatment consisted of excision of the aneurysm, ligation of the external carotid, and realization of a common to internal carotid artery bypass with the greater saphenous vein ...201323880460
salmonella enteritidis infections associated with foods purchased from mobile lunch trucks--alberta, canada, october 2010-february 2011.during october 2010-february 2011, an outbreak of 91 salmonella enteritidis (se) infections in alberta, canada, was investigated by a local public health department (alberta health services, calgary zone). index cases initially were linked through a common history of consumption of food purchased from mobile food-vending vehicles (lunch trucks) operating at worksites in alberta. further investigation implicated one catering company that supplied items for the lunch trucks and other vendors. in 8 ...201323863703
survival of salmonella enteritidis on four types of stainless steel surface under a dry condition and recovery by swabbing.the survival and recovery of salmonella enteritidis inoculated on stainless steel surfaces with different metal contents and surface finishes were examined. two s. enteritidis strains possessing different levels of biofilm productivity were inoculated with tryptone soya broth (tsb) and egg yolk emulsion (ey) on the surface of stainless steel squares (1 cm × 1 cm) and stored at 22℃ under a dry condition. after storage, cells were recovered from the stainless steel surfaces by swabbing with a cott ...201323863367
[salmonella enteritidis infection complicated by acute myocarditis]. 201323849316
diversity among human non-typhoidal salmonellae isolates from zimbabwe.non-typhoidal salmonella infections are an important public health problem in sub-saharan africa, especially among children and hiv-seropositive patients in whom they may cause invasive disease.201323843558
a temporal study of salmonella serovars from fluff samples from poultry breeder hatcheries in ontario between 1998 and 2008.the objectives of this study were to determine the prevalence of salmonella, trends, major serovars, and their clusters from fluff samples, in poultry hatcheries in ontario between 1998 and 2008. multi-level logistic regression modelling with random effects for hatchery and sampling visit (day on which samples were collected from a hatchery) was used to identify factors [poultry breeder type, year (trend), and season] associated with the prevalence of salmonella and a cluster detection test was ...201323814352
a temporal study of salmonella serovars from environmental samples from poultry breeder flocks in ontario between 1998 and 2008.a temporal study was carried out to determine salmonella prevalence, trends, major serovars, and their clusters from environmental samples, in poultry breeder flocks in ontario between january 1998 and december 2008. surveillance data were obtained from the ontario hatchery and supply flock policy. logistic regression with a random effect for flock was used to identify factors [poultry type, year (trend) and season] associated with the prevalence of salmonella. a cluster detection test was used ...201323814351
[detection of foodborne salmonella typhimurium and salmonella enteritidis by diplex real-time pcr using taqman probe].to establish a method to detect salmonella typhimurium (st) and salmonella enteritidis (se) simultaneously with a dual real-time pcr assay using double-color fluorescent taqman probes.201323805534
prediction of salmonella enteritidis growth in pasteurized and unpasteurized liquid egg products with a growth model.growth prediction of a four-strain cocktail of salmonella enteritidis in commercial products of pasteurized and unpasteurized liquid whole egg was studied with the new logistic model that we developed. the growth data of the pathogen in the liquid egg products at constant temperatures in our recent study (sakha and fujikawa, biocont. sci., 2012) were used for prediction. with estimated values of the parameters in the model, it successfully predicted the salmonella growth in the liquid egg produc ...201323796640
application of ph-sensitive fusogenic polymer-modified liposomes for development of mucosal vaccines.to evaluate the usefulness of ph-sensitive fusogenic polymer (succinylated poly(glycidol) (sucpg) and 3-methylglutarylated poly(glycidol) (mglupg))-modified liposomes as mucosal vaccine in the induction of a protective immune responses was evaluated. mice were nasally immunized with ova-containing sucpg-modified liposomes. after immunization, significant ag-specific abs were detected in the serum and intestine. when sera were analyzed for isotype distribution, antigen-specific igg1 ab responses ...201423790647
immune regulation of ovarian development: programming by neonatal immune challenge.neonatal immune challenge by administration of lipopolysaccharide (lps) produces enduring alterations in the development and activity of neuroendocrine, immune and other physiological systems. we have recently reported that neonatal exposure to an immune challenge by administration of lps results in altered reproductive development in the female wistar rat. specifically, lps-treated animals exhibited diminished ovarian reserve and altered reproductive lifespan. in the current study, we examined ...201323781169
sustained protection in mice immunized with fractional doses of salmonella enteritidis core and o polysaccharide-flagellin glycoconjugates.non-typhoidal salmonella (nts) serovars s. enteritidis and s. typhimurium are a major cause of invasive bacterial disease (e.g., bacteremia, meningitis) in infants and young children in sub-saharan africa and also occasionally cause invasive disease in highly susceptible hosts (young infants, the elderly, and immunocompromised subjects) in industrialized countries. no licensed vaccines exist against human nts infections. nts core and o polysaccharide (cops) and flic (phase 1 flagellin subunits) ...201323741368
combined effect of nisin, carvacrol and a previous thermal treatment on the growth of salmonella enteritidis and salmonella senftenberg.the aim of this study was to evaluate the combined effect of a previous mild heat treatment (15 min at 55 ) with the use of antimicrobials, nisin and carvacrol, on the growth of salmonella enteritidis and salmonella senftenberg. natural antimicrobials, alone or combined with a previous mild heat treatment, affected the growth of these two serovars in tryptone soy broth at 37 . increasing concentrations of carvacrol had a significant effect on both growth rate and lag phase duration of both strai ...201323729416
[an etiological survey on a foodborne disease epidemic outbreak caused by salmonella enteritidis].to conduct an etiological molecular epidemiological survey and laboratory test on a foodborne disease epidemic outbreak to make clear of the cause and implement effective prevention and control on it.201323719109
ruptured cryptogenic mycotic abdominal aortic aneurysm by salmonella enteritidis.the aim of this study is to describe a case of ruptured cryptogenic mycotic abdominal aortic aneurysm by salmonella enteritidis (se) and present a comprehensive review of the literature.201323706179
detection of salmonella enteritidis in pooled poultry environmental samples using a serotype-specific real-time-polymerase chain reaction assay.while real-time-polymerase chain reaction (rt pcr) has been used as a rapid test for detection of salmonella enteritidis in recent years, little research has been done to assess the feasibility of pooling poultry environmental samples with a salmonella enteritidis-specific rt pcr assay. therefore the objective of this study was to compare rt pcr salmonella enteritidis detection in individual and pooled (in groups of two, three, and four) poultry environmental drag swab samples to traditional cul ...201323678725
assessment of the anti-salmonella activity of commercial formulations of organic acid products.organic acid products are widely used in the uk poultry industry as feed or water additives. claims for salmonella control are made for some of these products, but there are few studies comparing the anti-salmonella effect of the various products in tests that reflect field application. the present studies examined the effects of 13 commercial blends (four water products and nine feed products) on salmonella enteritidis and typhimurium strains. initial screening, in nutrient broth, of all produc ...201323600468
functional validation of the genetic architecture of salmonella enteritidis persistence in 129s6 mice.the gram-negative bacteria, salmonella, cause a broad spectrum of clinical diseases in humans, ranging from asymptomatic carriage to life-threatening sepsis. we have designed an experimental model to study the contribution of genetic factors to the persistence of salmonella enteritidis during the late phase of infection in 129s6/svevtac and c57bl/6j mice. c57bl/6j mice cleared the bacteria from their reticuloendothelial system within a period of 42 days, whereas the 129s6 mice still presented a ...201323588612
an improved non-crosslinking gold nanoprobe-nasba based on 16s rrna for rapid discriminative bio-sensing of major salmonellosis pathogens.in the absence of an appropriate detection method for simple and rapid differentiation of major salmonellosis-causing agents, nano-bio-sensing could provide ideal molecular detection approaches for food-borne pathogens. in addition, the 16s ribosomal rna-based detection techniques would enhance specificity and sensitivity due to the presence of multiple copies of 16s rrna per cells and also the presence of hypervariable regions in the genes. in this study, we developed a novel detection techniqu ...201323584228
salmonella spp. in patients suffering from enteric fever and food poisoning in thamar city, yemen.salmonella remains a public health concern around the world, including yemen although data on its incidence are few. this study determined the incidence of salmonella infection in 250 enteric fever and 210 food poisoning cases attending thamar general hospital and dar alshafaa medical clinic in 2008. in total, 773 clinical specimens were taken: 250 blood, 187 urine and 336 stool samples. of the patients with enteric fever and food poisoning, 16.4% and 15.2% respectively were infected with salmon ...201323520911
antimicrobial resistance in invasive non-typhoid salmonella from the democratic republic of the congo: emergence of decreased fluoroquinolone susceptibility and extended-spectrum beta lactamases.co-resistance against the first-line antibiotics ampicillin, chloramphenicol and trimethoprim/sulphamethoxazole or multidrug resistance (mdr) is common in non typhoid salmonella (nts). use of alternative antibiotics, such as fluoroquinolones or third generation cephalosporins is threatened by increasing resistance, but remains poorly documented in central-africa.201323516651
evaluation of anti-se bacteriophage as feed additives to prevent salmonella enteritidis (se) in broiler.this experiment was conducted to evaluate anti-salmonella enteritidis (anti-se) bacteriophage as feed additives to prevent salmonella enteritidis in broilers. the experimental diets were formulated for 2 phases feeding trial, and 3 different levels (0.05, 0.1 and 0.2%) of anti-se bacteriophage were supplemented in basal diet. the basal diet was regarded as the control treatment. a total of 320 1-d-old male broilers (ross 308) were allotted by randomized complete block (rcb) design in 8 replicate ...201325049801
intact but not denatured ovine serum immunoglobulins positively modulate mucosal immune mediators in the growing rat challenged with salmonella enteritidis.immunoglobulins are major glycoproteins that modulate the immune response of gut-associated lymphoid tissue. in the present study, we sought to determine whether orally administered ovine serum immunoglobulins modulate selected indices of mucosal immune function and immune mediators in the growing rat challenged with salmonella enteritidis. rats were fed a casein-based basal control diet (bd; unchallenged). three groups of rats were challenged orally with 1 x 10(7) viable s. enteritidis on day 1 ...201323433493
hypopituitarism occurring in neonatal sepsis.we report here a case of acquired hypopituitarism in a female neonate with salmonella enteritidis sepsis and cystic encephalomalacia, after prolonged dopamine administration. severe hyponatremia was suggestive of hypopituitarism development and the later was documented by hormonal status. this is the first report of infectious hypophysitis in neonatal age. the careful monitoring of pituitary function should be recommended for septic patients exposed to prolonged use of dopamine.201223426810
efficacy for a new live attenuated salmonella enteritidis vaccine candidate to reduce internal egg contamination.to evaluate the efficacy of a novel attenuated salmonella enteritidis (△lon△cpxr) vaccine candidate (jol919), chickens were immunized through oral and intramuscular routes to reduce egg contamination against s. enteritidis challenge. birds were orally immunized with jol919 on the first day of life and were subsequently boosted in the 6th and 16th weeks through oral (group b) or intramuscular (group c) route, while control birds were unimmunized (group a). the chickens of all groups were challeng ...201423409934
invasive non-typhoidal salmonella infection with multifocal seeding in an immunocompetent host: an emerging disease in the developed world.we report an immunocompetent 24-year-old man who presented with a severe, invasive non-typhoidal salmonella (ints) infection. he presented with lumbar back pain associated with fever and rigours, which had been preceded by diarrhoea. blood cultures grew salmonella enteritidis. an mri scan of his pelvis and spine showed that he had a small gluteal abscess and sacroiliitis. his condition subsequently deteriorated due to the development of a secondary pneumonia and respiratory failure. he was manag ...201323370956
investigation of an excess of salmonella enteritidis phage type 14b and mlva type 4-7-3-13-10-2-2 in luxembourg, belgium and germany during 2010.we investigated an increase of human cases of salmonella enteritidis occurring from august until november 2010 in belgium, luxembourg and germany involving an estimated three hundred laboratory confirmed cases. molecular typing indicated that the increase in luxembourg and belgium was due a particular strain having phage type 14b, mlva pattern 4-7-3-13-10-2-2 and fully susceptible to the enternet panel of antibiotics. mlva and phage typing were found to have similar discriminatory power on a col ...201223362566
salmonella sternoclavicular osteomyelitis in a patient with crohn's disease.we describe a 73-year-old man with crohn's disease and previous sternotomies, who developed salmonella sternoclavicular osteomyelitis subsequent to a salmonella enteritidis sepsis and closed fracture of his clavicle. we include evidence from several cases related to sternoclavicular osteomyelitis, and salmonella osteomyelitis. we continue by summarising the aetologies of these diseases, and risk factors that predispose to them.201323345484
salmonella enteritidis deposition in eggs after experimental infection of laying hens with different oral doses.the continuing attribution of human salmonella enteritidis infections to internally contaminated eggs has necessitated the commitment of substantial public and private resources to salmonella enteritidis testing and control programs in commercial laying flocks. cost-effective risk-reduction requires a detailed and comprehensive understanding of how salmonella enteritidis infections in hens result in deposition of the pathogen inside eggs. the present study sought to resolve some incompletely def ...201323317864
a review on development of novel strategies for controlling salmonella enteritidis colonization in laying hens: fiber-based molt diets.limiting salmonella enteritidis from table eggs can involve intervention approaches at several levels of the production cycle, beginning at the hatchery and ending at the processing or table egg production facilities. likewise, interventions that limit salmonella enteritidis dissemination can be implemented at various stages during the life cycle of infection of salmonella in the laying hen. however, achieving complete elimination of salmonella infestation in egg products has remained elusive. t ...201323300320
protective effect of mannan oligosaccharides against early colonization by salmonella enteritidis in chicks is improved by higher dietary threonine levels.to evaluate mannan oligosaccharide (mos) and threonine effects on performance, small intestine morphology and salmonella spp. counts in salmonella enteritidis-challenged birds.201323279100
growth characteristics of salmonella enteritidis in pasteurized and unpasteurized liquid egg products.growth characteristics of a four-strain cocktail of salmonella enteritidis in commercial products of pasteurized and unpasteurized liquid whole egg were studied with a growth model developed by us. the unpasteurized product contained total bacteria at 10(7.3) cfu/g, but no salmonella. when the products were spiked with salmonella at various doses ranging from 10(1) to 10(4) cfu/g, growth curves of the pathogen at 24°c were well described with our model. salmonella growth curves at constant tempe ...201223269220
salmonella detection in poultry samples. comparison of two commercial real-time pcr systems with culture methods for the detection of salmonella spp. in environmental and fecal samples of poultry.the efficiency of two commercial pcr methods based on real-time technology, the foodproof® salmonella detection system and the bax® pcr assay salmonella system was compared to standardized culture methods (en iso 6579:2002 - annex d) for the detection of salmonella spp. in poultry samples.201223242148
salmonella enteritidis induced myocarditis in a 16-year-old girl.myocarditis typically presents with non-specific clinical symptoms, and can easily be missed in the absence of a high index of clinical suspicion. myocarditis caused by bacterial pathogens is rare in immunocompetent individuals, more commonly seen following viral infection. although more classically associated with typhoid fever and gastroenteritis, salmonella species are a rare cause of myocarditis. we report a case of salmonella enteritidis-induced myocarditis after gastrointestinal infection ...201223188875
recurrent meningitis due to salmonella enteritidis: a case report from kashmir india.recurrent bacterial meningitis in children is potentially life-threatening and induces psychological trauma to the patients through repeated hospitalization. here we report a case of recurrent meningitis in a one month old baby. the csf and blood culture grew salmonella enteritidis. injection ciprofloxacin and ceftriaxone were given for 3 weeks. baby became symptomatically better and was afebrile at discharge. twenty eight days after discharge baby got readmitted with complaints of fever and ref ...201323183477
a 2010 austrian salmonella enteritidis pt4 outbreak associated with a laying hen holding previously involved in an s. enteritidis pt4 cluster: pitfalls of regulatory responses in risk management.we report on an outbreak caused by salmonella enteritidis phage type 4 (pt4) among 143 participants at a soccer camp in austria in august 2010. the outbreak affected 34 persons, including 24 epidemiologically related cases and 10 laboratory-confirmed cases. food-specific cohort analyses revealed spaetzle (homemade noodles) (relative risks (rr): 2.68; 95% ci: 1.13-6.45), hamburger (rr: 2.70; 95% ci: 1.13-6.45) and potato salad (rr: 2.91; 95% ci: 1.69-5.02) as the most biologically plausible infec ...201223164561
study of the effect exerted by fructo-oligosaccharides from yacon (smallanthus sonchifolius) root flour in an intestinal infection model with salmonella typhimurium.beneficial effects of prebiotics like inulin and fructo-oligosaccharides (fos) have been proven in health and nutrition. yacon (smallanthus sonchifolius), an andean crop, contains fos (50–70% of its dry weight) and, therefore, is considered a prebiotic. commercial fos can upregulate total secretory iga (s-iga) in infant mice, prevent infection with salmonella in swine or enhance immune response for salmonella vaccine in a mouse model. previously, we found that administration of yacon root flour ...201323137694
recurrent prosthetic joint infection due to salmonella enteritidis: case report and literature review.prosthetic joint infection (pji) due to salmonella spp. is rare. we report a case of a recurrent prosthetic infection with involvement of two joints in a 72-year-old man and review the literature. the review of all cases previously reported and our case shows that conservative approach using antibiotics alone is insufficient to cure the infection. prolonged antibiotic treatment and two-stage reconstruction with the use of joint spacer is the best choice for the treatment. salmonella spp. should ...201226662756
evaluating risk factors for endemic human salmonella enteritidis infections with different phage types in ontario, canada using multinomial logistic regression and a case-case study approach.identifying risk factors for salmonella enteritidis (se) infections in ontario will assist public health authorities to design effective control and prevention programs to reduce the burden of se infections. our research objective was to identify risk factors for acquiring se infections with various phage types (pt) in ontario, canada. we hypothesized that certain pts (e.g., pt8 and pt13a) have specific risk factors for infection.201223057531
detection of salmonella enteritidis (se) bacterin in environmental samples from poultry houses using the rapidchek se immunoassay.environmental swabs from pullet houses were tested for the presence of salmonella enteritidis (se) using the rapidchek se immunoassay test. of the 1162 samples tested in 2011, 20 samples were positive, but only two samples were confirmed positive for se by culture. seventeen positive samples were from pullet houses that had been vaccinated with se bacterin 2 to 3 days prior to submission to the lab. this study investigated the detection of se bacterin using the rapidchek se test system. swabs we ...201223050486
[mycotic pseudoaneurysm produced by salmonella enteritidis in a patient with fever of unknown origin]. 201623044283
molecular characterization of salmonella enteritidis: comparison of an optimized multi-locus variable-number of tandem repeat analysis (mlva) and pulsed-field gel electrophoresis.salmonella enteritidis (se) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. this study describes the development and evaluation of an optimized multiple-locus variable number tandem-repeat assay (mlva) for characterization of se. the typeability and discriminatory power of this mlva was determined on a selected collection of 60 se isolates and compared with pulsed-field gel electrophoresis (pfge) using restriction enzymes xbai ...201223035725
sanitization efficacy of anaerobic digestion and aeration of slurry from the aspect of limiting emission of salmonella into the environment.the aim of this study was to estimate the usefulness of mesophilic anaerobic digestion and aeration for sanitization of slurry from the aspect of limiting transmission of salmonella into the environment. material for the study was fresh pig slurry. collected samples were subjected to anaerobic digestion at 35°c and aeration with an initial temperature of 35°c. the efficacy of both methods was examined based on determination of the elimination rate and theoretical time of survival of salmonella s ...201223020034
microbial-resistant salmonella enteritidis isolated from poultry samples.multidrug resistance in salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain.201226989702
cutaneous leukocytoclastic vasculitis due to salmonella enteritidis in a child with interleukin-12 receptor beta-1 deficiency.defects in the interleukin 12 (il-12)/interferon gamma (ifn-γ) pathway result in mendelian susceptibility to mycobacterial disease (msmd). il-12 receptor beta 1 (il-12rβ1) deficiency, the most common form of msmd, is associated with weakly virulent mycobacteria and salmonella. infections in patients with this deficiency are extraintestinal, or septicemic, recurrent infections with nontyphoid salmonellae. here we report a case of an il-12rβ1 deficiency with cutaneous leukocytoclastic vasculitis d ...201423004925
serotypes of salmonella isolated from faeces of patients with acute diarrhoea in gwangju area, korea, during 2000-2009.the purpose of this study was to determine the changing pattern of salmonella serotypes causing acute diarrhoea in humans in gwangju area, korea, during 2000-2009. a total of 596 salmonella isolated from culture of 29,896 faecal samples of patients with acute diarrhoea were included in this study. faecal samples were collected from local hospitals and clinics in gwangju area during january 2000-december 2009. the mean annual frequency of isolates for the 10 years was 2.0% (range, 0.9-6.0). the i ...201222958339
salmonella mycotic aneurysm: a rare cause of fever and back pain in elderly.an 85-year-old man with history of hypertension presented with fever, cough and abdominal pain. unfortunately fever and leukocytosis persisted despite treatment. blood cultures obtained on admission grew salmonella enteritidis. subsequently he developed increasing back pain and transoesophageal echocardiography (toe) was performed, demonstrating a complex ulcer at the aortic arch with multiple small mobile strandlike densities suggestive of vegetation. contrast thoracic ct scan confirmed an ulce ...201322918572
heat stress impairs performance and induces intestinal inflammation in broiler chickens infected with salmonella enteritidis.stressful situations reduce the welfare, production indices and immune status of chickens. salmonella spp. are a major zoonotic pathogens that annually cause over 1 billion infections worldwide. we therefore designed the current experiment to analyse the effects of 31±1°c heat stress (hs) (from 35 to 41 days) on performance parameters, salmonella invasion and small intestine integrity in broiler chickens infected with salmonella enteritidis. we observed that hs decreased body weight gain and fee ...201222900578
lipopolysaccharide prolongs action potential duration in hl-1 mouse cardiomyocytes.sepsis has deleterious effects on cardiac function including reduced contractility. we have shown previously that lipopolysaccharides (lps) directly affect hl-1 cardiac myocytes by inhibiting ca(2+) regulation and by impairing pacemaker "funny" current, i(f). we now explore further cellular mechanisms whereby lps inhibits excitability in hl-1 cells. lps (1 μg/ml) derived from salmonella enteritidis decreased rate of firing of spontaneous action potentials in hl-1 cells, and it increased their pa ...201222895260
salmonella aortitis treated with endovascular aortic repair: a case report.salmonella is a typical cause of aortitis, which is associated with high morbidity and mortality. in infrarenal disease, besides open surgery, endovascular aortic repair as an alternative treatment has been reported. to the best of our knowledge, we report the first successful endovascular aortic repair documented by necropsy to date.201222894684
[surveillance and analysis on the pathogenic features of salmonella in guangdong province in 2010].in order to better understand the nature of salmonella infection in diarrheal patients in guangdong province, the study analyzed the serum types, antibiotic resistance and molecular determinants of the isolated salmonella strains.201222883729
transmission and shedding patterns of salmonella in naturally infected captive wild roof rats (rattus rattus) from a salmonella-contaminated layer farm.rodents play a major role in the transmission and maintenance of salmonella contamination cycles in poultry facilities. however, very limited field data are available regarding the transmission routes, infection cycle, and shedding patterns of salmonella by naturally infected wild rodents from commercial layer farms. in this study, a total of 128 resident wild roof rats (rattus ratus) were captured from a salmonella-contaminated layer facility. all roof rats were divided into 51 laboratory cages ...201222856184
development of a new loop-mediated isothermal amplification assay for prt (rfbs) gene to improve the identification of salmonella serogroup d.loop-mediated isothermal amplification (lamp) is a promising nucleic acid assay for rapid and cost-effective detection of pathogen-specific sequences within a sample. development of an appropriate taxonomic group-specific lamp assay highly relies on the design of proper primers to cover all major members of the taxon. regarding this fact, we designed and evaluated a new lamp primer set specific to prt (rfbs) gene for rapid identification of salmonella serogroup d serotypes. unlike the previously ...201222806032
blood heat shock proteins evoked by some salmonella strains infection in ducks.bacterial heat-shock response is a global regulatory system required for effective adaptation to changes (stress) in the environment. an in vitro study was conducted to investigate the impact of a sublethal temperature (42°c) on heat shock protein (hsp) expression in 6 salmonella strains (salmonella enteritidis, s. typhimurium, s. virchow, s. shubra, s. haifa and s. eingedi). the 6 salmonella strains were isolated from the tissues of ducklings that had died from avian salmonellosis. to determine ...201222806021
national surveillance of salmonella enteritidis in commercial eggs in japan.a total of 105 033 eggs were collected across japan from june 2010 to january 2011 and tested for salmonella enteritidis to provide data for the risk profiling of s. enteritidis in eggs by the food safety commission of japan. s. enteritidis isolates were recovered from three samples (20 eggs/sample) and these samples were different in regard to sampling period, grading and packaging centre and farm. the prevalence of s. enteritidis in commercial eggs in japan is estimated at ~0.003% which was a ...201322793254
salmonella enteritidis bacteremia with septic arthritis of the sacroiliac joint in a patient with systemic lupus erythematosus: case report and review of the literature.an 18-year-old female presented with a ten days history of high grade fever, chills and pain of the left sacroiliac joint. the patient has systemic lupus erythematosus (sle) and is on chronic immunosuppressive therapy (steroids, antimalarial and antimetabolites). imaging of the left sacroiliac joint revealed inflammation. blood cultures and an aspirate of a small gluteal abscess that she developed later grew salmonella enteritidis resistant to nalidixic acid. the patient was treated conservative ...201222746014
epidural abscess due to salmonella enteritidis in a patient with systemic lupus erythematosus.increased susceptibility to infection has long been observed among patients with systemic lupus erythematosus, and still represents a chief cause of morbidity and mortality in these patients. this is due in part to the severity of infection and to the difficulty of early diagnosis owing to the similarity between sle flares and infection. epidural infection is an uncommon condition, and a most rare condition caused by salmonella, which accounts for a broad spectrum of human illnesses from gastroe ...201222736747
severe infections caused by salmonella enteritidis pt8/7 linked to a private barbecue.a cohort study on a barbecue-associated salmonella outbreak was conducted to describe the burden of disease and to identify the outbreak vehicle. dose-response relationships were tested with fisher's exact and wilcoxon rank sum tests (alpha = 0·05). s. enteritidis isolates were cultured and phage-typed. information was available for 11 out of 14 individuals attending the barbecue; all were healthy young adults (median age 27 years). the attack rate was 100%. three cases were hospitalized and two ...201322717224
estimating the true incidence of campylobacteriosis and salmonellosis in the european union, 2009.we estimated the true incidence of campylobacteriosis and salmonellosis in the european union (eu) in 2009. the estimate was based on disease risks of returning swedish travellers, averaged over the years 2005-2009, and anchored to a dutch population-based study on incidence and aetiology of gastroenteritis. for the 27 eu member states the incidence of campylobacteriosis was about 9·2 (95% ci 2·8-23) million cases, while the incidence of salmonellosis was 6·2 (95% ci 1·0-19) million cases. only ...201322717051
[salmonellosis outbreaks and the size and role of the chilean state].during year 2011 two outbreaks of salmonella infection captured media attention in the metropolitan area (ma) in chile: one of typhoid fever associated to salmonella serotype typhi, and the other, of gastroenteritis related to salmonella serotype enteritidis, both with decreasing or stable rates in the previous years. the aim of this work is to analyze probable causes of their reemergence.201222689037
[clinical and epidemiological description of severe outbreak of foodborne infection by salmonella enteritidis].foodborne diseases have increased considerably.201222689025
[salmonella enteritidis bacteraemia as clinical onset of acquired immune deficiency syndrome].the case is presented of a 38 year-old patient who was admitted in the emergency department due to a severe acute respiratory failure and who was transferred to the critical care unit with a suspected initial diagnosis of community acquired pneumonia caused by an atypical microorganism, which was complicated with an acute respiratory distress syndrome. this was able to be treated with non-invasive mechanical ventilation. at 48 hours after admission, the growth of gram negative bacilli in the blo ...201322657351
investigation of the concurrent emergence of salmonella enteritidis in humans and poultry in british columbia, canada, 2008-2010.an increase in the rate of human infections with salmonella enteritidis (se) occurred between 2007 and 2010 in british columbia (bc). during the same time period, an increase in se from poultry-sourced isolates and increased clinical severity in poultry were also observed in bc. this article describes a multi-sectoral collaboration during a 3-year investigation, and the actions taken by public health and animal health professionals. human cases were interviewed, clusters were investigated, and a ...201222639971
the effects of sage extract feed supplementation on biochemical parameters, weight of internal organs and salmonella counts in chickens.we investigated the effects of dietary addition of sage extract on the biochemical parameters, weight of some body organs and changes in the counts of salmonella enteritidis pt4 (se) in experimentally infected chickens. the following diets were used: basal diet, basal diet with addition of an extract of salvia officinalis l. (s), basal diet and se, and basal diet and s and se (sse). compared to the se group, sage extract in the sse group decreased activities of alp and alt and concentrations of ...201222587934
[salmonella enteritidis is a rare cause of neck abscesses].a case of a neck abscess caused by salmonella enteritidis in a 48 year-old diabetic patient is presented. salmonella neck abscesses are rare and often occur in association with infection in other parts of the body. the condition is treated with drainage and antibiotics. it should be included in the differential diagnosis of abscesses of the head and neck - especially in immunosuppressed patients.201222564692
the epidemiology of travel-related salmonella enteritidis in ontario, canada, 2010-2011.increases in the number of salmonellosis cases due to salmonella enteritidis (se) in 2010 and 2011 prompted a public health investigation in ontario, canada. in this report, we describe the current epidemiology of travel-related (tr) se, compare demographics, symptoms and phage types (pts) of tr and domestically-acquired (da) cases, and estimate the odds of acquiring se by region of the world visited.201222537320
promoting crystallisation of the salmonella enteritidis fimbriae 14 pilin sefd using deuterium oxide.the use of heavy water (d(2)o) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. a significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. here we illustrate the use of d(2)o in additive screening for the production of reproducible diffraction-quality crystals for the salmonella enteritidis fimbriae 14 (sef14) putative tip adhesin, sefd.201222497887
[necrotizing fasciitis caused by salmonella enteritidis].a 73 year-old immunocompromised male was admitted to the intensive care unit with signs of necrotising fasciitis and septic shock. prompt surgery was performed. cultures showed salmonella enteritidis. relevant antibiotics were given in a course of 14 days. a few weeks later the patient had a relapse of fever. again blood cultures showed s. enteritidis. this case presents a rare manifestation of extraintestinal infection with s. enteritidis, and implicates the need for long-term antibiotic therap ...201222487410
development of a sequence typing scheme for differentiation of salmonella enteritidis strains.a dna sequence typing scheme based on the caic and sen0629 loci was developed for differentiation of salmonella enteritidis strains and validated using a diverse collection of 102 isolates representing 38 phage types from different sources, year of isolation, geographical locations and epidemiological backgrounds. caic encodes a probable crotonobetaine/carnitine-coa ligase, and sen0629 is a pseudogene. our system allowed for discrimination of 16 sequence types (sts) among the 102 isolates analys ...201222486191
dietary supplementation with ovine serum immunoglobulin attenuates acute effects on growth, organ weights, gut morphology and intestinal mucin production in the growing rat challenged with salmonella enteritidis.the aim was to determine the effect of orally administered ovine serum immunoglobulin (ig) on growth performance, organ weight, gut morphology and mucin production in the salmonella enteritidis--gavaged growing rat. four groups consisted of non-gavaged rats fed a casein-based control basal diet (bd) and three groups of rats gavaged with 1×10(7) cfu s. enteritidis and fed a casein-based diet, a diet containing freeze-dried ovine ig (fdoi) or a casein-based diet containing inactivated ovine ig (io ...201122440348
survival of salmonella in home-style mayonnaise and acid solutions as affected by acidulant type and preservatives.mayonnaise made from contaminated eggs has been linked to outbreaks of salmonella infections. this study was undertaken to determine the fate of salmonellae in home-style mayonnaise and acid solutions with or without chemical preservatives. egg yolks were inoculated with different levels of a three-serotype (typhimurium, heidelberg, and enteritidis [untypeable phage type]) mixture of salmonella or a three-phage-type (4, 8, and 13) mixture of salmonella enteritidis. the inoculated yolks were used ...201222410219
propylparaben sensitizes heat-resistant salmonella enteritidis and salmonella oranienburg to thermal inactivation in liquid egg albumen.propyl p-hydroxybenzoic acid (propylparaben [prpa]) is a phenolic antioxidant, known to occur in nature and used as a microbiostat in foods, feeds, pharmaceuticals, cosmetics, and medications. the u.s. department of agriculture, food safety and inspection service (fsis) requires that liquid egg white (lew) be pasteurized at 56.7°c for 3.5 min. this study evaluated the effects of prpa on the pasteurization sensitivity of salmonella in lew. when lew (ph 7.8) was pasteurized under fsis conditions, ...201222410216
investigation of rpos and dps genes in sodium hypochlorite resistance of salmonella enteritidis se86 isolated from foodborne illness outbreaks in southern brazil.in rio grande do sul, southern brazil, salmonella enteritidis is one of the principal microorganisms responsible for foodborne disease. the present study was conducted to compare the sodium hypochlorite resistance of salmonella enteritidis se86 with that of other strains of salmonella enteritidis isolated from different regions of the world and to investigate the involvement of the rpos and dps genes in resistance to this disinfectant. we tested five salmonella enteritidis wild-type (wt) strains ...201222410215
transfer of salmonella enteritidis to four types of surfaces after cleaning procedures and cross-contamination to tomatoes.the objectives of the present study were to evaluate the spread of salmonella enteritidis to different cutting boards (wood, triclosan-treated plastic, glass, and stainless steel) from contaminated poultry skin (5 log cfu/g) and then to tomatoes and to analyze the effect of different protocols used to clean these surfaces to control contamination. the following procedures were simulated: (1) no cleaning after handling contaminated poultry skin; (2) rinsing in running water; (3) cleaning with dis ...201222365360
inactivation of salmonella spp. in liquid whole egg using pulsed electric fields, heat, and additives.this paper evaluates the lethal effectiveness on 7 different salmonella serovars of the application, in static and continuous conditions, of pulsed electric fields (pef) followed by heat treatments in liquid whole egg (lwe) with additives (edta or triethyl citrate-tc-). compared to heat treatments, the pef (25 kv/cm and 75-100 kj/kg) followed by heat (52°c/3.5', 55°c/2', or 60°c/1') in lwe with 2% tc permitted the reduction of heat treatment time from 92 fold at 52°c to 3.4 fold at 60°c, and 4.8 ...201222365352
transgenerational transmission of anxiety induced by neonatal exposure to lipopolysaccharide: implications for male and female germ lines.neonatal lipopolysaccharide (lps) exposure increases anxiety-like behaviour and alters neuroendocrine responses to stress in adult rats. the current study assessed whether this anxiety-related phenotype observed in rats neonatally exposed to lps is transferable to subsequent generations. wistar rats were exposed to lps (0.05 mg/kg, salmonella enteritidis) or non-pyrogenic saline (equivolume) on postnatal days 3 and 5. in adulthood, animals were subjected to restraint and isolation stress or no s ...201222342246
high pressure treatments on the inactivation of salmonella enteritidis and the physicochemical, rheological and color characteristics of sliced vacuum-packaged dry-cured ham.the effect of high pressure (hp) on salmonella enteritidis in sliced dry-cured ham stored under temperature abuse (8°c) during 60d was investigated. after treatment, reductions of s. enteritidis were 1.06, 2.54 and 4.32 log units in ham treated at 400, 500 and 600mpa for 5min at 12°c, compared to non-pressurized samples. after 60d, counts of s. enteritidis in ham treated at 400 and 500mpa were 2.56 and 2.66 log units lower than in non-treated ham, whereas the pathogen was only detected after enr ...201222330945
variation in salmonella enteritidis rapd-pcr patterns may not be due to genetic differences.salmonella enteritidis is a leading cause of gastroenteritis associated with consumption of contaminated poultry meat and eggs. because pulsed-field gel electrophoresis (pfge) has limited utility in distinguishing between clonal salmonella enteritidis isolates, random amplified polymorphic dna (rapd) pcr has been recommended as an alternative molecular fingerprinting tool. this study's objective was to determine whether increasing pcr stringency would improve the repeatability of rapd dna patter ...201122312982
colonization of a marker and field strain of salmonella enteritidis and a marker strain of salmonella typhimurium in vancomycin-pretreated and nonpretreated laying hens.this study was conducted to evaluate the influence of a vancomycin pretreatment on the ability of marker (nalidixic-acid resistant) salmonella enteritidis (se(m)), field salmonella enteritidis (se(e)), and marker salmonella typhimurium (st(m)) strains to colonize within the intestinal and reproductive tracts and translocate to other organs of leghorn laying hens. in each of three trials, caged laying hens (76, 26, and 33 wk ofage) were divided into six groups designated to receive se(m), se(f), ...201122312978
development of rna aptamers for detection of salmonella enteritidis.we developed and evaluated rna aptamers to analyze their potential for use in detecting salmonella enteritidis. the selected aptamer was observed to specifically bind to salmonella enteritidis without any cross-reactivity to other salmonella serovars. thus, this study suggests that aptamers specific to salmonella enteritidis have a high potential for use in presumptive presumptive screening methods or alternative serotyping methods.201222310030
classification and structural analysis of live and dead salmonella cells using fourier transform infrared spectroscopy and principal component analysis.fourier transform infrared spectroscopy (ft-ir) was used to detect salmonella typhimurium and salmonella enteritidis food-borne bacteria and to distinguish between live and dead cells of both serotypes. bacteria cells were prepared in 10(8) cfu/ml concentration, and 1 ml of each bacterium was loaded individually on the znse attenuated total reflection (atr) crystal surface (45° znse, 10 bounces, and 48 mm × 5 mm effective area of analysis on the crystal) and scanned for spectral data collection ...201222257216
splenic abscess due to salmonella enteritidis.splenic abscess is a very rare complication of non-typhoidal salmonella infections. we report a case of splenic abscess caused by salmonella enteritidis. the patient is a 63-year-old woman with diabetes mellitus and underwent splenectomy. this case suggests that the patients with comorbities are at increased risk for invasive infections in non-typhoidal salmonella infections.201224470934
[phage types recognized within salmonella enteritidis strains isolated in poland in 1996-2007].phage typing carried out according to the well-defined schemes is still recommended as a standard, fast and inexpensive method for epidemiological investigations all over the world to control salmonella infections. however, the method should be in the hands of well-trained staff. this means that it is generally limited to reference laboratories.201223484389
behavior of salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns.over the years, salmonella heidelberg (sh) has gained prominence in north america poultry production and in the poultry production of other countries. salmonella heidelberg has been isolated and reported from poultry and poultry products in brazil since 1962, whereas salmonella enteritidis (se) has only emerged as a serious problem in poultry and public health since 1993. these strains of salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. ...201124031631
[rare complications of salmonella enteritidis infection].we report 64-year old patient who was admitted in icu with left side ileofemoral thrombosis and splenic abscess during salmonella enteritidis infection.200923120852
[toxin food production by salmonella enterítidis]. 200528272389
cfd approach for modelling microbial inactivation during thermal processing of intact eggs.combined conductive and convective heat transfer during thermal pasteurisation of intact eggs was studied using a commercial computational fluid dynamics (cfd) package. a kinetic inactivation model for salmonella enteritidis was combined with the cfd model, enabling evaluation and assessment of egg pasteurisation processes. simulated temperature profiles were found to be in good agreement with experimentally observed data. the obtained results offer a useful tool for assessment and optimisation ...200324757782
genetic resistance to mortality of day-old chicks and carrier-state of hens after inoculation with salmonella enteritidis.the heritability of resistance of poultry to salmonella enteritidis (se) was investigated. three measurements of resistance were made: survival after intramuscular inoculation of 419 day-old chicks, absence versus presence of salmonella in spleens and caeca 4 weeks after oral inoculation of 304 hens at peak of laying, and antibody response of 228 hens following two inoculations of an aroa mutant of this serotype. in the first two models of infection, resistance appeared to be heritable. the heri ...199926911499
outbreak of salmonella food poisoning at high altitude.an outbreak of food poisoning occurred among soldiers of an army unit located at high altitude (3300m) in western himalayas. a total of 78 of the 103 soldiers who had consumed the incriminated meal suffered from the illness. sixteen of the 78 had symptoms severe enough to warrant admission to hospital. the symptoms consisted of diarrhoea, vomiting, fever, headache, vertigo and abdominal cramps of varying intensity. salmonella enteritidis was isolated from 6 stool samples and in one blood sample. ...199828775436
salmonella septicaemia in a patient with burns.a report of a case of salmonella enteritidis which caused septicaemia in a patient who had suffered major burns.199227911173
antibiotic interrelationships among the enteric group of bacteria. 194716561421
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