Publications

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lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces.five strains of thermotolerant lactic acid bacteria (g 12, g 22, g 35t, g 43 and g 44) isolated from chicken faeces were characterized taxonomically. the strains were facultatively anaerobic, gram-positive, catalase-negative, non-motile, non-spore-forming rods. they were heterofermentative lactobacilli that produced dl-lactic acid. growth of the strains occurred at 45 degrees c but not at 15 degrees c. the optimum temperature for growth was 42 degrees c, as determined from the specific growth ra ...200312656183
lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons.lactic acid bacteria were isolated from the crop and intestines of pigeons. one group of strains, showing similar genomic patterns after screening with trna intergenic spacer pcr, could not be identified to the species level. sequencing of the 16s rrna gene of one representative strain revealed about 96% similarity to sequences from lactobacillus fermentum and lactobacillus mucosae. determination of the dna base composition, dna-dna hybridization experiments, sds-page of whole-cell proteins and ...200312656164
development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, lactobacillus thermotolerans, in chicken feces.a new real-time pcr assay was successfully developed using a taqman fluorescence probe for specific detection and enumeration of a novel bacterium, lactobacillus thermotolerans, in chicken feces. the specific primers and probe were designed based on the l. thermotolerans 16s rrna gene sequences, and these sequences were compared to those of all available 16s rrna genes in the genbank database. the assay, targeting 16s rrna gene, was evaluated using dna from a pure culture of l. thermotolerans, d ...200516085805
reclassification of lactobacillus thermotolerans niamsup et al. 2003 as a later synonym of lactobacillus ingluviei baele et al. 2003.the relatedness of the species lactobacillus ingluviei and lactobacillus thermotolerans was investigated by comparing partial sequences of the 16s rrna gene (99.9 % similarity over 1504 bp), the hsp60 gene (98.8 % similarity over 954 bp) and the reca gene (98.5 % similarity over 452 bp) and by determining dna-dna binding levels (79+/-3 %) and genomic dna g+c contents (50 and 49 mol%, respectively). these data, in addition to their similar biochemical characteristics, suggest that the two taxa co ...200616585696
lactobacillus secaliphilus sp. nov., isolated from type ii sourdough fermentation.two strains of gram-positive, catalase-negative, lactic acid bacteria, strains tmw 1.1309(t) and tmw 1.1313, were isolated at an interval of several years from an industrial type ii sourdough. they occurred at cell numbers of 8x10(8) c.f.u. g(-1) and therefore were considered to be one of the dominant members of the microbiota in this type of fermentation. cells of both strains grow exclusively on modified mrs containing trypsin-digested rye-bran extract. both strains possessed identical 16s rrn ...200717392199
microbial community composition of the crop and ceca contents of laying hens fed diets supplemented with chlorella vulgaris.it is generally accepted that the intestinal microbiota plays an important role in sustaining health and productivity of animals. chlorella vulgaris, a naturally occurring green microalga, is believed to influence performance and health, including bird reproduction and egg quality. the nutritive value of open or indoor cultured c. vulgaris depends upon the technological process used to treat the algal mass. in the present paper, it is presented and discussed how 2 differentially processed c. vul ...200919834082
diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores.the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ...201021034840
lactobacillus alvi sp. nov., isolated from the intestinal tract of chicken.strain r54(t) was isolated from the gizzard of hens. the isolate was gram-positive, facultative anaerobic, gas-forming, catalase-negative, nonmotile, nonspore-forming and short-rod-shaped. the optimal temperature for growth was 40 °c and the dna g+c content was 42.7 mol%. the 16s rrna gene sequences similarity showed that strain r54(t) was most closely related to lactobacillus ingluviei lmg 20380(t) (97.5%), followed by lactobacillus coleohominis cip 106820(t) (96.1%), lactobacillus secaliphilus ...201122092683
An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice.Food can modify the intestinal flora, and Lactobacillus ingluviei has been shown to cause weight gain in chicks and ducks but not in mammals.201222020311
genome sequence of lactobacillus ingluviei, a bacterium associated with weight gain in animals.we report the draft genome sequence of lactobacillus ingluviei strain autruche 4 (csurp209) isolated from an ostrich. l. ingluviei is associated with weight gain in mice. this genome sequence may help us understand the obesity-induced mechanisms of intestinal bacteria.201223012279
comparative meta-analysis of the effect of lactobacillus species on weight gain in humans and animals.obesity is associated with alteration of the gut microbiota. in order to clarify the effect of lactobacillus-containing probiotics (lcp) on weight we performed a meta-analysis of clinical studies and experimental models. we intended to assess effects by lactobacillus species.201222634320
comparison of microbial diversity of korean commercial makgeolli showing high β-glucan content and high antihypertensive activity, respectively.we measured physiological functionalities, including antihypertensive angiotensin i-converting enzyme inhibitory activity and immun-stimulating β-glucan content for sixty kinds of makgeolli that is commercially available from the market. as a result, we selected r-12 commercial raw makgeolli, with a high content of immuno-stimulating β-glucan, and r-14 commercial raw makgeolli, exhibiting high antihypertensive activity. due to the similarities in their overall physicochemical properties and raw ...201222870058
effect of newly isolated lactobacillus ingluviei adk10, from chicken intestinal tract on acetaminophen induced oxidative stress in wistar rats.the total antioxidative activity of l. ingluviei adk10 isolated from chicken intestine intact cells and cell free culture supernatant (cfcs) was 54- 67.95%. the ability to scavenge a,a-diphenyl-b-picrylhydrazyl free radical ranged from 71 and 64% in intact cells and cfcs respectively. total reducing activity of bacteria was equivalent to 290 microm/l of cysteine. reducing glutathione activity was equivalent to 93.95 microg/ml. oral administration of the strain at a dose of 10(9) cfu/kg body weig ...201323923611
succession of the turkey gastrointestinal bacterial microbiome related to weight gain.because of concerns related to the use of antibiotics in animal agriculture, antibiotic-free alternatives are greatly needed to prevent disease and promote animal growth. one of the current challenges facing commercial turkey production in minnesota is difficulty obtaining flock average weights typical of the industry standard, and this condition has been coined "light turkey syndrome" or lts. this condition has been identified in minnesota turkey flocks for at least five years, and it has been ...201324432198
identification of lactobacillus strains of goose origin using maldi-tof mass spectrometry and 16s-23s rdna intergenic spacer pcr analysis.the objective of our study was to identify lactobacillus sp. strains of goose origin using maldi-tof mass spectrometry, its-pcr and its-pcr/rflp. all three techniques proved to be valuable tools for identification of avian lactobacilli and produced comparable classification results. lactobacillus strains were isolated from 100% of geese aged 3 weeks to 4 years, but from only 25% of chicks aged 1-10 days. among the 104 strains isolated, we distinguished 14 lactobacillus species. the dominant spec ...201424607713
screening of lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics.lactobacilli are natural inhabitants of human and animal mucous membranes, including the avian gastrointestinal tract. recently, increasing attention has been given to their probiotic, health-promoting capacities, among which their antagonistic potential against pathogens plays a key role. a study was conducted to evaluate probiotic properties of lactobacillus strains isolated from feces or cloacae of domestic geese. among the 104 examined isolates, previously identified to the species level by ...201425104766
phenotypic and genotypic characterization of some lactic acid bacteria isolated from bee pollen: a preliminary study.in the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. all isolates were screened for their antagonistic activity against both gram-positive and gram-negative pathogenic bacteria. neutralized supernatants of 54 lactic acid bacteria (lab) cultures from 216 active isolates inhibited the growth of indicator bacteria. they were phenotypically characterized, based on the fermentation of 39 carbohydrates. using the simple matching ...201424936378
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