| center of origin and centers of diversity in an ancient crop, brassica rapa (turnip rape). | brassica rapa is the most widely distributed and has the longest history of domestication of the agricultural brassica species. molecular genetic diversity, based on 51 simple sequence repeat primer pairs and 715 alleles at polymorphic loci, was used to predict the center of origin and centers of diversity in a global collection of 173 b. rapa accessions. the accessions were separated into 3 molecular genetic groups based on structure analysis-group 1 from the classical old world (europe and wes ... | 2017 | 24714366 |
| [isolation and characterization of bcmf3, a gene expressed only in maintainer line in chinese cabbage-pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee)]. | expression profiling was performed using cdna-aflp technology on floral buds of a genic male sterile line (a line) and a maintainer line (b line) in chinese cabbage pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee cv. aijiaohuang). a differentially expressed cdna fragment, ba18-t16, was obtained via selective amplification with a18/t16 primer pair. ba18-t16 was specifically associated with the maintainer line, and was expressed only in floral buds revealed by half ... | 2004 | 15651684 |
| trace gases generated in closed plant cultivation systems and their effects on plant growth. | interactions between plants and trace gases, especially ethylene, were investigated from two different viewpoints; ethylene is toxic for plant growth, whereas the ethylene release rate of plants can be utilized as a plant growth indicator. when lettuce plants and shiitake mushroom mycelium were cultivated in closed chambers, ethylene concentration increased with time. ethylene was released both from lettuce plant and from shiitake mushroom mycelium. dioctyl phthalate (dop) and dibutyl phthalate ... | 1995 | 11541892 |
| seed predation, pathogen infection and life-history traits in brassica rapa. | herbivory and disease can shape the evolution of plant populations, but their joint effects are rarely investigated. families of plants of brassica rapa (brassicaceae) were grown from seeds collected in two naturalized populations in an experimental garden. we examined leaf infection by the fungus alternaria, seed predation by a gall midge (cecidomyiidae) and plant life-history traits. plants from one population had heavier seeds, were more likely to flower, had less fungal infection, had more s ... | 1995 | 28306843 |
| relationship between protoplast size and critical field strength in protoplast electropulsing and application to reliable dna uptake in brassica. | protoplast diameter, a physical parameter controlling the susceptibility to electropermeation, was observed to vary between different batches of brassica napus and brassica campestris protoplasts. in order to control the permeation field strength associated with the protoplast size, we investigated the mathematical relationship between permeant field strength and protoplast size by measuring electroloading of the calcein dye under conditions that did not alter protoplast survival. a roughly line ... | 1991 | 24221493 |
| characterization of bcmf23a and bcmf23b, two putative pectin methylesterase genes related to pollen development in brassica campestris ssp. chinensis. | two homologous genes, brassica campestris male fertility 23a (bcmf23a) and brassica campestris male fertility 23b (bcmf23b), encoding putative pectin methylesterases (pmes) were isolated from brassica campestris ssp. chinensis (syn. brassica rapa ssp. chinensis). these two genes sharing high sequence identity with each other were highly expressed in the fertile flower buds but silenced in the sterile ones of genic male sterile line system ('bcajh97-01a/b'). results of rt-pcr and in situ hybridiz ... | 2017 | 27844189 |
| striking sequence similarity in inter- and intra-specific comparisons of class i slg alleles from brassica oleracea and brassica campestris: implications for the evolution and recognition mechanism. | self-incompatibility in brassica is controlled by a single multi-allelic locus (s locus), which contains at least two highly polymorphic genes expressed in the stigma: an s glycoprotein gene (slg) and an s receptor kinase gene (srk). the putative ligand-binding domain of srk exhibits high homology to the secretory protein slg, and it is believed that slg and srk form an active receptor kinase complex with a self-pollen ligand, which leads to the rejection of self-pollen. here, we report 31 novel ... | 1997 | 9207151 |
| microrna expression analysis of rosette and folding leaves in chinese cabbage using high-throughput solexa sequencing. | in this study, a global analysis of mirna expression from rosette leaves (rls) and folding leaves (fls) of chinese cabbage (brassica rapa l. ssp. pekinensis) was conducted using high-throughput solexa sequencing. in total, over 12 million clean reads were obtained from each library. sequence analysis identified 64 conserved mirna families in each leaf type and 104 and 95 novel mirnas from rls and fls, respectively. among these, 61 conserved mirnas and 61 novel mirnas were detected in both types ... | 2013 | 24055726 |
| instability in mitochondrial membranes in polima cytoplasmic male sterility of brassica rapa ssp. chinensis. | cytoplasmic male sterility (cms) is an important factor to observe heterosis in brassica rapa. although several studies have documented the rearrangements of mitochondrial dna and dysfunction in the mitochondria have been observed in most types of cms, the basis of the molecular mechanisms involved in these processes and other effects on cms remain unclear. in this study, suppression subtractive hybridization was performed in the flowers of an alloplasmic polima cms system from b. rapa ssp. chin ... | 2014 | 24652098 |
| host-associated genetic structure of mexican populations of the cabbage aphid brevicoryne brassicae l. (homoptera: aphididae). | phytophagous insects can use different host plant species across their geographic distribution. within a locality, however, their feeding can be restricted to one or two plant species. if host species constitute different selective regimes to herbivorous insects, genetic differentiation and host-associated local adaptation may occur. in this study, we describe the genetic structure of the aphid brevicoryne brassicae l. associated to brassica campestris l. and b. oleraceae var. capitata l., two p ... | 2003 | 14512958 |
| morphological, cytological, and molecular characterization of hybrids and their progenies derived from the somatic hybridization of brassica campestris and brassica oleracea. | in order to produce interspecific somatic hybrids between brassica campestris (2n = 20, aa) and brassica oleracea (2n = 18, cc), we isolated protoplasts from cotyledons and hypocotyls of young seedlings, and fused by 40% polyethylene glycol (peg). fused cells were cultured in modified k8p liquid medium supplemented with 0.2 mg/l 2,4-dichorophenoxyacetic acid (2,4-d) +0.5 mg/l 6-benzylaminopurine (6-ba)+0.1 mg/l naphthaleneacetic acid (naa)+ 0.1 mg/l kinetin (kin), 0.3 mol/l sucrose and 0.3 mol/l ... | 2011 | 22393713 |
| synthesis and herbicidal activity of novel 3-aminocarbonyl-2-oxazolidinethione derivatives containing a substituted pyridine ring. | a series of 3-aminocarbonyl-2-oxazolidinethione derivatives containing a substituted pyridine ring were designed and synthesized. the structures of all of the title compounds were characterized by 1h nmr, 13c nmr, ir, and hrms. their agricultural bioactivities were evaluated, and some of these compounds exhibited good herbicidal activities against echinochloa crusgalli, sorghum vulgare, digitaria sanguinalis, eclipta prostrasta, cucumis sativus, and brassica campestris, which were associated mai ... | 2006 | 16390188 |
| maize grain concentrations and above-ground shoot acquisition of micronutrients as affected by intercropping with turnip, faba bean, chickpea, and soybean. | most research on micronutrients in maize has focused on maize grown as a monocrop. the aim of this study was to determine the effects of intercropping on the concentrations of micronutrients in maize grain and their acquisition via the shoot. we conducted field experiments to investigate the effects of intercropping with turnip (brassica campestris l.), faba bean (vicia faba l.), chickpea (cicer arietinum l.), and soybean (glycine max l.) on the iron (fe), manganese (mn), copper (cu) and zinc (z ... | 2013 | 23900569 |
| effects of humic acid on phytodegradation of petroleum hydrocarbons in soil simultaneously contaminated with heavy metals. | the use of humic acid (ha) to enhance the efficiency of phytodegradation of petroleum hydrocarbons in soil contaminated with diesel fuel was evaluated in this study. a sample of soil was artificially contaminated with commercially available diesel fuel to an initial total petroleum hydrocarbons (tph) concentration of 2300 mg/kg and four heavy metals with concentrations of 400 mg/kg for pb, 200 mg/kg for cu, 12 mg/kg for cd, and 160 mg/kg for ni. three plant species, brassica campestris, festuca ... | 2011 | 22432335 |
| effect of different fertilizers on nitrogen isotope composition and nitrate content of brassica campestris. | the effect of different fertilizers on the δ(15)n value, nitrate concentration, and nitrate reductase activity of brassica campestris and the δ(15)n value of soil has been investigated through a pot experiment. the δ(15)n mean value of b. campestris at the seedling stage observed in the composted chicken treatment (+8.65‰) was higher than that of chemical fertilizer treatment (+5.73‰), compost-chemical fertilizer (+7.53‰), and control check treatment (+7.86‰). there were significantly different ... | 2012 | 22242784 |
| mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line. | a brassica campestris-alboglabra monosomic addition line (genome: aa + one chromosome from the c genome, 2n = 21) harbours the brassica alboglabra (cc, 2n = 18) chromosome with the gene for erucic acid. in order to identify this chromosome, we have studied the mitotic prometaphase chromosomes of brassica campestris (aa, 2n = 20), b. alboglabra, and the monosomic addition line. more pronounced differential staining and size differences of chromosomes were observed in b. campestris than in b. albo ... | 1995 | 18470170 |
| random amplified polymorphic dna markers of the brassica alboglabra chromosome of a b. campestris-alboglabra addition line. | the alien c-genome chromosome in a brassica campestris alboglabra monosomic addition line was characterized by random amplified polymorphic dna (rapd) analysis. the alien chromosome carried three loci, ec, wc and lap-1c, controlling synthesis of erucic acid, white flower colour and a fast-migrating band of leucine aminopeptidase (lap-1cc) respectively. the rapd analysis revealed 17 markers specific to the alien chromosome. among 45 offspring plants from the selfed addition line the alien c-chrom ... | 1996 | 8785604 |
| erratum: mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line. | | 1995 | 18470209 |
| patulibacter brassicae sp. nov., isolated from rhizosphere soil of chinese cabbage (brassica campestris). | a novel actinobacterial strain, designated sdt, was isolated from rhizosphere soil of chinese cabbage in shandong province, china. the cells were aerobic, gram-staining-positive, oxidase- and catalase-positive, short rods and formed white colonies on trypticase soy agar. the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine, glutamic acid and leucine. diphosphatidylglycerol was the predominant polar lipid. the predominant cellular fatty acid wa ... | 2016 | 27620694 |
| impact and correlation of environmental conditions on pollen counts in karachi, pakistan. | a quantitative and qualitative survey of airborne pollen was performed in the city of karachi, and the pollen counts were correlated with different climatic conditions. the aim of the study was to determine the possible effect of meteorological factors on airborne pollen distribution in the atmosphere of karachi city. pollen sampling was carried out by using burkard spore trap for the period of august 2009 to july 2010, and a total of 2,922 pollen grains/m(3) were recorded. in this survey, 22 po ... | 2015 | 25530143 |
| roseomonas soli sp. nov., isolated from an agricultural soil cultivated with chinese cabbage (brassica campestris). | a bacterial strain, designated 5n26(t), was isolated from an agricultural soil cultivated with chinese cabbage (brassica campestris). cells of this strain were gram-reaction-negative, strictly aerobic, motile, non-spore-forming rods, and catalase- and urease-negative. the major fatty acids of strain 5n26(t) were c16 : 0 (7.5 %), c18 : 1 2-oh (13.4 %) and summed feature 8 (c18:1ω6c and/or c18:1ω7c; 63.2%). the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidy ... | 2014 | 24363292 |
| anaerobically digested poultry slaughterhouse wastes as fertiliser in agriculture. | chemical and physical analysis, 27-d plant growth assays with carrot (daucus carota) and chinese cabbage (brassica campestris var. chinensis), and 5-d phytotoxicity assays with chinese cabbage and perennial ryegrass (lolium perenne) were used to investigate the suitability of anaerobically digested poultry slaughterhouse waste for fertiliser in agriculture and the effect of aerobic post-treatment on the properties of the digested material. the digested material appeared to be rich in nitrogen. i ... | 2001 | 11265792 |
| expression and occurrence of uracil-dna glycosylase in higher plants. | uracil-dna glycosylase (udg) is the first enzyme in the base excision repair pathway for removal of uracil in dna. dna repair capacity is likely to be a critical factor in mutagenesis and thereby in the capacity to prevent genetic damage and unwanted variation. we have studied expression of udg in 9 higher plant species. the highest expression of udg was measured in solanum tuberosum. a comparison of 6 solanum tuberosum cultivars showed that the specific activity ranged from 30 pmol mg1 protein ... | 1993 | 28741780 |
| generation and identification of brassica alboglabra-brassica campestris monosomic alien addition lines. | four monosomic alien addition lines (maals) for brassica alboglabra-brassica campestris were developed through digenomic triploid (acc) backcrossing with the recurrent parent b. alboglabra (cc). the objectives of this study were to compare morphological traits, microsatellite markers (simple sequence repeats), chromosomal karyotypes, and meiotic behaviors. based on the new chromosome nomenclature system established for brassica, we preliminarily identified these maals as cc+a1, cc+a3, cc+a6, and ... | 2013 | 23659701 |
| isolation and characterization of a phosphorus-solubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage (brassica campestris ssp. chinensis). | phosphate-solubilizing bacteria (psb) can promote the dissolution of insoluble phosphorus (p) in soil, enhancing the availability of soluble p. thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development. from the rhizosphere of chinese cabbage plants grown in yangling, we isolated a strain of psb (yl6) with a strong ability to dissolve p and showed that this strain promoted the growth of these plants under field conditions. however, systemati ... | 2017 | 28798725 |
| abscisic acid treatment alleviates cadmium toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis var. purpurea hort.) seedlings. | the aim of this research was to investigate how exogenous abscisic acid (aba) alleviates cadmium (cd) toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis) and evaluate whether it could be a potential choice for phytoremediation. purple flowering stalk seedlings were cultivated in a hydroponic system with cd at various concentrations (0-100 μmol l-1) as controls and cd plus aba as the treatment in the growth media. the soluble proteins, chlorophyll contents and the activity ... | 2017 | 28755629 |
| elevation of secondary metabolites synthesis in brassica campestris ssp. chinensis l. via exogenous inoculation of piriformospora indica with appropriate fertilizer. | this work evaluated the impact of exogenous soil inoculation of beneficial fungal strain piriformospora indica on phytochemical changes and the related genes expression of chinese cabbage (brassica campestris ssp. chinensis l.) by greenhouse pot experiments. high performance liquid chromatography (hplc) affirmed that among the different combinations of fungal and organic fertilizer treatments, the phenolic acids and flavonoids were considerably enriched in organic fertilizer and fungi (op) follo ... | 2017 | 28493970 |
| transcriptomic profiling of two pak choi varieties with contrasting anthocyanin contents provides an insight into structural and regulatory genes in anthocyanin biosynthetic pathway. | the accumulation of anthocyanin in horticultural crops not only improves their stress tolerances but also their nutritional values. many key regulatory and structural genes in anthocyanin biosynthesis have been identified in model plants, but limited information is available for non-model plant species featured with colored leaves. in this study, two pak choi varieties with green or purple leaves were selected to analyze the anthocyanin biosynthesis through rna-seq. | 2017 | 28399809 |
| over-expression of mir158 causes pollen abortion in brassica campestris ssp. chinensis. | we identified and cloned the two precursors of mir158 and its target gene in brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. further study revealed that over-expression of mir158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. these results first suggest the role of mir158 in pollen development of brassica campestris ssp. chinensis. micrornas (mirnas) play crucial role ... | 2017 | 27909970 |
| the effectiveness of spent coffee grounds and its biochar on the amelioration of heavy metals-contaminated water and soil using chemical and biological assessments. | spent coffee grounds (scg) and charred spent coffee grounds (scg-char) have been widely used to adsorb or to amend heavy metals that contaminate water or soil and their success is usually assessed by chemical analysis. in this work, the effects of scg and scg-char on metal-contaminated water and soil were evaluated using chemical and biological assessments; a phytotoxicity test using bok choy (brassica campestris l. ssp. chinensis jusl.) was conducted for the biological assessment. when scg and ... | 2014 | 25242543 |
| expression analysis of self-incompatibility-associated genes in non-heading chinese cabbage. | in brassicaceae, a self-incompatibility (si) system mediates pollen-pistil interactions. self-pollen could be recognized and rejected by incompatible pistils. several components involved in the si response have been determined, including s-locus receptor kinase (srk), s-locus cysteine-rich protein/s-locus protein 11, and arm repeat-containing protein 1 (arc1). however, the components involved in the si system of brassicaceae are not fully understood. here, we detected expression patterns of 24 s ... | 2014 | 25062491 |
| transcriptomic analysis of pak choi under acute ozone exposure revealed regulatory mechanism against ozone stress. | ground-level ozone (o3) is one of the major air pollutants, which cause oxidative injury to plants. the physiological and biochemical mechanisms underlying the responses of plants to o3 stress have been well investigated. however, there are limited reports about the molecular basis of plant responses to o3. in this study, a comparative transcriptomic analysis of pak choi (brassica campestris ssp. chinensis) exposed to different o3 concentrations was conducted for the first time. | 2017 | 29216819 |
| identification and fine mapping of a stay-green gene (brnye1) in pakchoi (brassica campestris l. ssp. chinensis). | using bulked segregant analysis combined with next-generation sequencing, we delimited the brnye1 gene responsible for the stay-green trait of nye in pakchoi. sequence analysis identified bra019346 as the candidate gene. "stay-green" refers to a plant trait whereby leaves remain green during senescence. this trait is useful in the cultivation of pakchoi (brassica campestris l. ssp. chinensis), which is marketed as a green leaf product. this study aimed to identify the gene responsible for the st ... | 2017 | 29209732 |
| characterization and co-expression analysis of wrky orthologs involved in responses to multiple abiotic stresses in pak-choi (brassica campestris ssp. chinensis). | the wrky transcription factor is an important member of the stress-related transcription factors, which mediate diverse abiotic stresses in many plants. however, up until now, the number of wrky members, and the regulatory mechanisms involved in abiotic stress responses in pak-choi (brassica campestris ssp. chinensis), remained unknown. | 2013 | 24267479 |
| design, synthesis, and quantitative structure-activity relationship study of herbicidal analogues of pyrazolo[5,1-d][1,2,3,5]tetrazin-4(3h)ones. | a series of pyrazolo[5,1-d][1,2,3,5]tetrazin-4(3h)one derivatives were designed, synthesized, and evaluated for their herbicidal activities where some of these compounds provided >80% control of brassica campestris at 10 microg/ml. quantitative structure-activity relationship studies were performed on these compounds using physicochemical parameters (electronic, verloop, or hydrophobic) as independent parameters and herbicidal activity as a dependent parameter, where herbicidal activity correlat ... | 2007 | 17300154 |
| brassica compestris var. span: i. fractionation of rapeseed oil by molecular distillation and adsorption chromatography. | procedures for the large scale isolation of pure triglycerides and fractions rich in nontriglyceride components from span rapeseed oil are described. fractionation of brassica campestris var. span rapeseed oil by molecular distillation yielded 4 triglyceride fractions, all of which contained traces of sterol esters. an additional triglyceride fraction rich in free and esterified sterols and other volatile components was obtained from the oil. separation by adsorption chromatography of span rapes ... | 1975 | 1177662 |
| molecular cloning and expression of the male sterility-related ctyabby1 gene in flowering chinese cabbage (brassica campestris l. ssp chinensis var. parachinensis). | expression of the yabby gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. however, the yabby gene has not been studied in male sterility and fertility restoration. we homologously cloned the ctyabby1 gene of male-sterile tc1 in brassica campestris l. ssp chinensis var. parachinensis; its expression was analyzed by real-time pcr. a 93 ... | 2014 | 25036178 |
| development of est-ssr markers in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) based on de novo transcriptomic assemblies. | flowering chinese cabbage is one of the most important vegetable crops in southern china. genetic improvement of various agronomic traits in this crop is underway to meet high market demand in the region, but the progress is hampered by limited number of molecular markers available in this crop. this study aimed to develop est-ssr markers from transcriptome sequences generated by next-generation sequencing. rna-seq of eight cabbage samples identified 48,975 unigenes. of these unigenes, 23,267 we ... | 2017 | 28902884 |
| directional transfer of a multiple-allele male sterile line in brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee. | to produce hybrid seeds of wutacai (brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee), a "directional transfer program" was designed to breed the multiple-allele male sterile line of wutacai. a multiple-allele male sterile line of naibaicai (brassica campestris l. ssp. chinensis l., s01) was used as the male sterile resource, and an inbred line of wutacai (wt01) was used as the target line. recurrent backcrossing was employed to transfer the male sterility and other b ... | 2014 | 24987301 |
| proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions. | the physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (gly-n) treatments were determined. two pakchoi (brassica campestris ssp. chinensis l. makino. var. communis tsen et lee) cultivars, 'huawang' and 'wuyueman', were grown under sterile hydroponic conditions with different n forms (gly-n and nitrate-n). gly-n-treated pakchoi exhibited decreased fresh weights, total n uptake, leaf areas, and net photosynthetic rates than those treated with nitrate ... | 2014 | 24429133 |
| composite of pah-degrading endophytic bacteria reduces contamination and health risks caused by pahs in vegetables. | vegetables accumulate polycyclic aromatic hydrocarbons (pahs) at high concentrations when grown in contaminated sites. inoculation with pah-degrading endophytic bacteria (ebpah) has been recognized as one of the most promising ways to remove pahs from plant bodies; however, the performance of single endophytic bacteria is generally limited. this investigation used a composite of eight ebpah to reduce the contamination and health risk posed by 16 epa priority pahs in vegetables including chinese ... | 0 | 28448936 |
| identification of novel and conserved mirnas involved in pollen development in brassica campestris ssp. chinensis by high-throughput sequencing and degradome analysis. | micrornas (mirnas) are endogenous, noncoding, small rnas that have essential regulatory functions in plant growth, development, and stress response processes. however, limited information is available about their functions in sexual reproduction of flowering plants. pollen development is an important process in the life cycle of a flowering plant and is a major factor that affects the yield and quality of crop seeds. | 2014 | 24559317 |
| characterization of the small rna transcriptome in plant-microbe (brassica/erwinia) interactions by high-throughput sequencing. | non-coding, small rnas (srnas) have been identified in a wide spectrum of organisms ranging from bacteria to humans; however, the role and mechanisms of these srna in plant immunity is largely unknown. to determine possible roles of srna in plant-pathogen interaction, we carried out a high-throughput srna sequencing of brassica campestris using non-infected plants and plants infected with erwinia carotovora. consistent with our hypothesis that distinct classes of host srnas alerts their expressi ... | 2014 | 24126536 |
| a repetitive sequence from diplotaxis erucoides is highly homologous to that of brassica campestris and b. oleracea. | | 1990 | 2103429 |
| comparison of ascorbic acid biosynthesis in different tissues of three non-heading chinese cabbage cultivars. | ascorbic acid (l-asa) is an important antioxidant in plants and humans. vegetables are one of the main sources of ascorbic acid for humans. for instance, non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is considered as one of the most important vegetables in south china. to elucidate the mechanism by which asa accumulates, we systematically investigated the expression profiles of d-mannose/l-galactose pathway-related genes. we also investigated the recycling-related genes ... | 2013 | 24157701 |
| identification of micrornas potentially involved in male sterility of brassica campestris ssp. chinensis using microrna array and quantitative rt-pcr assays. | micrornas (mirnas) are a class of newly identified, noncoding, small rna molecules that negatively regulate gene expression. many mirnas are reportedly involved in plant growth, development and stress response processes. however, their roles in the sexual reproduction mechanisms in flowering plants remain unknown. pollen development is an important process in the life cycle of a flowering plant, and it is closely related to the yield and quality of crop seeds. this study aimed to identify mirnas ... | 2013 | 23864334 |
| bcnrt1, a plasma membrane-localized nitrate transporter from non-heading chinese cabbage. | a nitrate transporter, bcnrt1, was isolated from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) cultivar 'suzhouqing'. the full-length cdna was obtained using the rapid amplification of cdna ends technique and contains an open reading frame of 1,770 bp that predicts a protein of 589 acid residues that possesses 12 putative transmembrane domains. using the gus marker gene driven by the bcnrt1 promoter, we found bcnrt1 expression to be concentrated in primary and lateral r ... | 2012 | 22539185 |
| reducing plant uptake of pahs by cationic surfactant-enhanced soil retention. | reducing the transfer of contaminants from soils to plants is a promising approach to produce safe agricultural products grown on contaminated soils. in this study, 0-400 mg/kg cetyltrimethylammonium bromide (ctmab) and dodecylpyridinium bromide (ddpb) were separately utilized to enhance the sorption of pahs onto soils, thereby reducing the transfer of pahs from soil to soil solution and subsequently to plants. concentrations of phenanthrene and pyrene in vegetables grown in contaminated soils t ... | 2009 | 19232798 |
| alleviation of heavy metal toxicity and phytostimulation of brassica campestris l. by endophytic mucor sp. mhr-7. | heavy metal (hm) pollution is of great concern in countries like pakistan where a huge proportion of human population is exposed to it. these toxic metals are making their way from water bodies to soil where it not only interferes with plant growth and development but also initiates serious health issues in human consuming the produce of such soils. bioremediation is one of the most viable and efficient solution for the problem. purpose of the current study was to isolate endophytic fungi from p ... | 2017 | 28407499 |
| microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading chinese cabbage. | microtubules are the basic components of the cytoskeleton in eukaryotic cells and are made up of 13 parallel protofilaments, each composed of α- and β-tubulin unit molecules aligned along the longitudinal axis of the microtubule. | 2012 | 22581783 |
| sequence characterization and expression pattern of bcmf21, a novel gene related to pollen development in brassica campestris ssp. chinensis. | in this report a full length cdna, brassica campestris male fertile 21 (bcmf21) was successfully isolated from one of the cdna-amplified fragment length polymorphism (cdna-aflp) transcript-derived fragments (tdfs), bbp10, that was found down-regulated in the flower buds of sterile plants in brassica campestris l. ssp. chinensis makino genic male sterile (gms) ab line system (bcajh 97-01a/b). bcmf21 protein structure analysis showed a signal peptide at the n-terminus; two protein kinase c phospho ... | 2012 | 22311045 |
| growth promotion of chinese cabbage and arabidopsis by piriformospora indica is not stimulated by mycelium-synthesized auxin. | piriformospora indica, an endophytic fungus of the order sebacinales, interacts with the roots of a large variety of plant species. we compared the interaction of this fungus with chinese cabbage (brassica campestris subsp. chinensis) and arabidopsis seedlings. the development of shoots and roots of chinese cabbage seedlings was strongly promoted by p. indica and the fresh weight of the seedlings increased approximately twofold. the strong stimulation of root hair development resulted in a bushy ... | 2011 | 21375386 |
| bcmf20, a putative pollen-specific transcription factor from brassica campestris ssp. chinensis. | the arabidopsis transcription factor (at1g26610) was shown to be down-regulated in male sterile lines compared to their maintainer line in chinese cabbage (brassica campestris ssp. chinensis) in our previous study. the bcmf20 gene, homologue of the at1g26610 gene was isolated from chinese cabbage. it encodes a putative c2h2 zinc finger protein. the spatial and temporal expression patterns examined by qrt-pcr and in situ hybridization, indicated bcmf20 is specifically expressed in the developing ... | 2011 | 21327825 |
| isolation and characterization of a novel bcmf14 gene from brassica campestris ssp. chinensis. | a putative ralf (rapid alkalinization factor)-like gene (genbank accession number ef523517), named bcmf14, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) by rapid amplification of cdna ends (race) based on a cdna-aflp differential fragment exclusively expressed in fertile line. semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) discovered that bcmf14 was prominently expressed in stage four and five flower buds ... | 2011 | 20857218 |
| a polygalacturonase inhibitory protein gene (bcmf19) expressed during pollen development in chinese cabbage-pak-choi. | the level of polygalacturonase inhibitory protein (pgip) genes involved in pollen development remains unclear. characterization of the different pgip genes that are expressed in pollen is necessary in understanding the similarities and differences of functions between the members of this gene family, as well as the underlying mechanism of pollen development. a gene-encoding putative pgip, bcmf19 was successfully cloned on a cdna-amplified fragment length polymorphism fragment after it was found ... | 2011 | 20373034 |
| differential expression of salt tolerance related genes in brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee. | we examined salt tolerance responsive genes in pak-choi under salt stress and analyze their potential function. the mrna differential display was used to screen the transcript derived fragments (tdfs) related to salinity tolerance in tolerant and moderately tolerant pak-choi germplasm. seventy-eight primer combinations generated 101 differential cdna fragments, which were divided into 10 expression types. seven cdna sequences (genbank accession nos. dq006915~dq006921) obtained and sequenced were ... | 2009 | 19882760 |
| the construction of a genetic linkage map of non-heading chinese cabbage (brassica campestris ssp. chinensis makino). | non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is one of the most important vegetables in eastern china. a genetic linkage map was constructed using 127 doubled haploid (dh) lines, and the dh population was derived from a commercial hybrid "hanxiao" (lines sw-13 x l-118). out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups(lgs). chi-square tests showed that 42.67% markers were distorted from expected mendelian segregation ratios, and ... | 2009 | 19683673 |
| molecular cloning and characterization of a novel pollen predominantly membrane protein gene bcmf12 from brassica campestris ssp. chinensis. | a novel membrane protein gene, bcmf12, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends based on a pollen-specific cdna fragment (dn237936). the cdna was 1,155 bp in length with an open reading frame of 894 bp capable of encoding a putative polypeptide of 297 amino acids with an estimated molecular mass of 34.6 kda and a predicted isoelectric point of 9.6. comparative and bioinformatics analyses revealed that bcmf12 showe ... | 2009 | 19169847 |
| chromosomal fragmentation: a possible marker for the selection of high gymnemic acid yielding accessions of gymnema sylvestre r. br. | gymnema sylvestre r. br. a member of family asclepiadaceae as mentioned in indian pharmacopoeia popular among the researchers because of stimulatory effect of its phytoconstituent on pancreatic cells and potential to treat type i and ii type of diabetes. | 2017 | 29142402 |
| transcriptomic analysis of the regulation of stalk development in flowering chinese cabbage (brassica campestris) by rna sequencing. | flowering chinese cabbage is a stalk vegetable whose quality and yield are directly related to stalk development. however, no comprehensive investigations on stalk development have been performed. to address this issue, the present study used rna sequencing to investigate transcriptional regulation at three key stages (seedling, bolting, and flowering) of stalk development in flowering chinese cabbage. anatomical analysis revealed that cell division was the main mode of stalk thickening and elon ... | 2017 | 29138433 |
| morphological and functional characterization of bcmf13 in the antisense-silenced plants of brassica campestris ssp. chinensis var. parachinensis. | the gene brassica campestris male fertility 13 (bcmf13, genbank accession number ef158459) was isolated as a reproductive organ-specific gene from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). it is exclusively expressed in stage four and five flower buds of fertile lines and is most strongly expressed in stamens. here, we report a functional characterization of this bcmf13 gene in the antisense-silenced plants. the inflorescence of the bcmf13 mutan ... | 2009 | 18592396 |
| transcriptional differences between the male-sterile mutant bcms and wild-type brassica campestris ssp. chinensis reveal genes related to pollen development. | a novel male-sterile mutant which lacks mature pollen, brassisa campestris male sterile (bcms), was identified in brassica campestris l. ssp. chinensis makino (syn. b. rapa ssp. chinensis). genetic analysis revealed that bcms was controlled by a single recessive mutation locus. genome-wide transcriptional profiling was performed on the flower buds of both the bcms mutant and the wild-type from which it originated, and profiling analysis indicated that there were numerous changes in gene expressi ... | 2008 | 18426481 |
| functional analysis of a pollen-expressed polygalacturonase gene bcmf6 in chinese cabbage (brassica campestris l. ssp. chinensis makino). | in our earlier work, a pollen-expressed polygalacturonase gene bcmf6 was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino) by cdna-amplified fragment length polymorphism (cdna-aflp) transcript profiling and rapid amplification of cdna ends (race). to unravel the biological function of bcmf6 gene, the antisense fragment from the bcmf6 gene with a9 promoter and camv35s promoter was transferred into flowering chinese cabbage (b. campestris ssp. chinensis var ... | 2008 | 18415101 |
| characterization of a male sterile related gene bcmf15 from brassica campestris ssp. chinensis. | data from cdna-aflp analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of brassica campestris l. ssp. chinensis makino, syn. b. rapa l. ssp. chinensis, indicated that mutation of the mmc gene resulted in changes in expression of a variety of genes. a transcript-derived fragment specifically accumulated in the wild-type flower buds was isolated, and the corresponding full-length cdna and dna was subsequentl ... | 2009 | 18034318 |
| characterization and functional analysis of a novel pcp gene bcmf5 from chinese cabbage (brassica campestris l. ssp. chinensis makino). | brassica campestris male fertile 5 (bcmf5), a novel member of the pollen coat protein class a (pcp-a) gene family, was identified from brassica campestris l. ssp. chinensis makino (chinese cabbage-pak-choi). temporal and spatial expression analysis showed that bcmf5 is a late-expressed pcp gene related to the process of determining pollen fertility. functional analysis by hairpin rna (hprna)-mediated rna interference also showed that the expression of bcmf5 is inhibited, which resulted in the lo ... | 2008 | 17945379 |
| [genetic model analysis of leaf-weight traits in non-heading chinese cabbage (brassica campestris ssp. chinensis makino)]. | the mixed major-gene plus polygene inheritance model was used to analyze the genetics of leafblade-weight and petiole-weight trait in sixqiu017 of non-heading chinese cabbage. the results showed that the leafblade-weight trait was controlled by one pair of negative complete dominant major genes plus additive-dominant polygene (d-4). the additive effect in the major gene was 1.8991, the dominant effect was -1.8991; the additive effect in the polygene was -1.2934, and the dominant effect was 1.793 ... | 2007 | 17855268 |
| identification of flavonoids and hydroxycinnamic acids in pak choi varieties (brassica campestris l. ssp. chinensis var. communis) by hplc-esi-msn and nmr and their quantification by hplc-dad. | twenty-eight polyphenols (11 flavonoid derivatives and 17 hydroxycinnamic acid derivatives) were detected in different cultivars of the chinese cabbage pak choi ( brassica campestris l. ssp. chinensis var. communis) by hplc-dad-esi-ms(n). kaempferol was found to be the major flavonoid in pak choi, glycosylated and acylated with different compounds. smaller amounts of isorhamnetin were also detected. a structural determination was carried out by (1)h and (13)c nmr spectroscopy for the main compou ... | 2007 | 17848079 |
| bcmf21 is important for pollen development and germination in brassica campestris ssp. chinensis. | brassica campestris male fertility 21 (bcmf21) was previously isolated from the flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. here, we used antisense rna technology to knock down the expression level of bcmf21 in b. campestris and analyzed the phenotype of the transgenic plants. alexander staining and scan ... | 2014 | 24323195 |
| the dynamic growth exhibition and accumulation of cadmium of pak choi (brassica campestris l. ssp. chinensis) grown in contaminated soils. | the accumulation of heavy metals, especially cadmium (cd), in leafy vegetables was compared with other vegetables. pak choi (brassica campestris l. ssp. chinensis) is a leafy vegetable consumed in taiwan and its safety for consumption after growing in contaminated soils is a public concern. a pot experiment (50 days) was conducted to understand the dynamic accumulation of cd by pak choi grown in artificially contaminated soils. the edible parts of pak choi were sampled and analyzed every 2-3 day ... | 2013 | 24284350 |
| effects of soil properties on heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) in pearl river delta, china. | this study was conducted to investigate the effects of soil properties on the heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) at the field scale. the concentrations of cadmium (cd), mercury (hg), and chromium (cr) in topsoil and vegetable samples from nanhai district of foshan city in the pearl river delta (prd) were analyzed. the results showed that 56.5% of the soil samples exceeded the grade ii of the chinese soil environme ... | 2007 | 17365337 |
| bcmf11, a putative pollen-specific non-coding rna from brassica campestris ssp. chinensis. | a full-length cdna, bcmf11, has been cloned from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends (race) based on a pollen-specific cdna fragment (dn237921). the bcmf11 cdna has a total length of 828bp with poly (a) tail. analysis of the sequence demonstrated that bcmf11 is a novel non-coding rna which has no prominent open reading frame (orf) or coding capacity. no significant similarities were observed between bcmf11 and previously publi ... | 2007 | 17207554 |
| [the ultrastructural observation of anthers of chinese cabbage's mail-sterility]. | the fertile and sterile anthers of a chinese cabbage (brassica campestris l. ssp. chinensis makino) were observed using electron microscope to find the ultrastructural feature of sterile anthers. the earliest abnormal phenomenon in sterile anther was nucleolus of sporogenous cells locating in the edge of nucleus. during microspore mother cell development, callus wall surrounding the cell displayed uneven in the thick ness and was discontinuous,and the some cytoplasm leaked out of the cell from s ... | 2005 | 16416967 |
| [calcium distribution in fertile and sterile anthers of a genic male sterile chinese cabbage]. | potassium antimonite was used to locate calcium in the fertile and sterile anthers of a genic male sterile chinese cabbage (brassica campestris l. ssp. chinensis makino) to probe the relation between ca(2+) and fertility and sterility of anthers of the cabbage. during fertile anther development, calcium granules increase in number in anther wall cells after meiosis, and then appeared also in locule, suggesting a calcium influx into locule from anther wall cells (plate i-4). then the number of ca ... | 2005 | 16361789 |
| functional analysis of a novel male fertility cyp86mf gene in chinese cabbage (brassica campestris l. ssp. chinensis makino). | in our earlier work, a cytochrome p450 cyp86mf gene was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa l.) by mrna differential display pcr (dd-pcr) and rapid amplification of cdna ends (race). to unravel the biological function of cyp86mf gene, the antisense fragment from the cyp86mf gene was transferred into chinese cabbage pak-choi (b. campestris ssp. chinensis var. communis tsen et lee). out of 22 plants transformed with the antisense ... | 2006 | 16075226 |
| role of nitrification inhibitor dmpp (3, 4-dimethylpyrazole phosphate) in no(3-)-n accumulation in greengrocery( brassica campestris l. ssp. chinensis) and vegetable soil. | the influence of nitrification inhibitor (ni) 3, 4-dimethylpyrazole phosphate (dmpp) on nitrate accumulation in greengrocery (brassica campestris l. ssp. chinensis) and vegetable soil at surface layer were investigated in field experiments in 2002 and 2003. results showed that ni dmpp took no significant effect on yields of edible parts of greengrocery, but it could significantly decrease no(3-)-n concentration in greengrocery and in vegetable soil at surface layer. in addition, ni dmpp could re ... | 2005 | 15900763 |
| plant wounding affects the oviposition of hellula undalis(f.). the impact of leaf chemistry. | crucifer specialists, such as the tropical pest hellula undalis (f.), developed a close association to their hosts and depend on characteristic secondary plant compounds, the glucosinolates (gs), in many cases. following tissue damage, gs undergo hydrolysis catalyzed by the enzyme myrosinase to produce a complex array of products which include isothiocyantes, cyanides, and thiocyanates depending on reaction condition and the chemical nature of the side chain. we found that mechanical plant damag ... | 2004 | 15759429 |
| identification of gene expression profile during fertilization in brassica campestris subsp. chinensis. | fertilization is controlled by a complex gene regulatory network. to study the fertilization mechanism, we determined time courses of the four developmental stages of fertilization in chinese cabbage pak-choi (brassica campestris subsp. chinensis) by cytological observation. we then used the arabidopsis ath1 microarray to characterize the gene expression profiles of pollinated and unpollinated pistils in b. campestris subsp. chinensis. the result showed 44 up-regulated genes and 33 down-regulate ... | 2013 | 23379337 |
| identification of differentially accumulating pistil proteins associated with self-incompatibility of non-heading chinese cabbage. | non-heading chinese cabbage (brassica campestris l. ssp. chinensis makino), an important vegetable crop in china, exhibits a typical sporophytic self-incompatibility (si) system. to better understand the mechanism of si response and identify potential candidate proteins involved in the si system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatib ... | 2014 | 23581423 |
| bcmf11, a novel non-coding rna gene from brassica campestris, is required for pollen development and male fertility. | key message : bcmf11 as a non-coding rna gene has an essential role in pollen development, and might be useful for regulating the pollen fertility of crops by antisense rna technology. we previously identified a 828-bp full-length cdna of bcmf11, a novel pollen-specific non-coding mrna-like gene from chinese cabbage (brassica campestris l. ssp. chinensis makino). however, little information is known about the function of bcmf11 in pollen development. to investigate its exact biological roles in ... | 2013 | 23064614 |
| [the cytochemical observation of anthers of chinese cabbage's male-sterile]. | a chinese cabbage (brassica campestris l. ssp. chinensis makino) produces 1/4 male sterile and 3/4 fertility in offspring. the sterile plant can be identified from the color of corolla that is some white when it grows out. the fertile and sterile anthers were researched using cytological and cytochemical methods. thick sections of both anthers of different developmental stages were stained with toluidine blue for general cytological observation and stained with the periodic-acid-schiff's (pas) t ... | 2004 | 15511072 |
| genotypic differences in effects of cadmium exposure on plant growth and contents of cadmium and elements in 14 cultivars of bai cai. | fourteen cultivars of bai cai (brassica campestris l. ssp. chinensis var. communis) were grown in the nutrient solutions containing 0-0.5 microg ml(-1) of cadmium (cd) to investigate genotypic differences in the effects of cd exposure on the plant growth and uptake and distribution of cd in bai cai plants. the cd exposure significantly reduced the dry and fresh weights of roots and shoots, the dry weight ratio of shoot/root (s/r), total biomass, and chlorophyll content (spad value). cd concentra ... | 2004 | 15473646 |
| [establishment of an efficient regeneration system for chinese cabbage cultured in vitro and histological observation of shoot development]. | an efficient plant regeneration system from explants of petiole with cotyledon was developed for chinese cabbage (brassica campestris ssp. chinensis makino) in our study. high frequency of shoot regeneration was obtained in the medium containing bap 2 mg/l, naa 0.45 mg/l and 5-7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium, with 84.3 percent being the highest frequency of shoot regeneration in seven chinese cabbage cultivars and one parentage, and the new formed shoo ... | 2004 | 15259989 |
| [reclamation of the plant regeneration efficiency of brassica campestris subsp. chinensis var.parachinensis]. | this investigation has developed an efficient and fast method for plant regeneration from petiole of cotyledon explants of brassica campestris l. subsp. chinensis makino var. parachinensis tsen et lee. a medium was designed for b. campestris subsp. chinensis var. parachinensis to obtain the high frequency of shoot regeneration, which contained bap 2 mg/l, naa 0.75-1.0 mgl and 7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium. 60 ml/l coconut milk were added to all of me ... | 2001 | 12549111 |
| statistical modeling for estimating glucosinolate content in chinese cabbage by growth conditions. | glucosinolate in chinese cabbage (brassica campestris l. ssp. pekinensis (lour.) rupr) has potential benefits for human health, and its content is affected by growth conditions. in this study, we used a statistical model to identify the relationship between glucosinolate content and growth conditions, and to predict glucosinolate content in chinese cabbage. | 2018 | 29315681 |
| the properties and dynamic changes of dom subfractions during food waste and sugarcane leaves co-composting. | the purpose of this research was to evaluate the properties and dynamic changes of humic acids (ha), fulvic acids (fa), hydrophobic neutrals (hon), and hydrophilic (hi) fractions of dissolved organic matter (dom) during food waste and sugarcane leaves co-composting process. the pools of ha, fa, hon, and hi were separated from dom by fractionation method, and characterized using spectroscopic (uv-vis, ftir) and pyrolysis-gc/ms analyses. the least peaks in the ha pool were found in ftir spectra wi ... | 2017 | 29280102 |
| increased biomass and quality and reduced heavy metal accumulation of edible tissues of vegetables in the presence of cd-tolerant and immobilizing bacillus megaterium h3. | a cd-resistant and immobilizing bacillus megaterium h3 was characterized for its impact on the biomass and quality and heavy metal uptake of edible tissues of two vegetables (brassica campestris l. var. aijiaohuang and brassica rapa l. var. shanghaiqing) grown in heavy metal-polluted soil. the impact of strain h3 on the soil quality was also evaluated. the increase in the edible tissue biomass and the contents of soluble proteins and vitamin c of the vegetables inoculated with strain h3 ranged f ... | 2018 | 29069614 |
| evaluation of leafy vegetables as bioindicators of gaseous mercury pollution in sewage-irrigated areas. | mercury (hg) can evaporate and enter the plants through the stomata of plant leaves, which will cause a serious threat to local food safety and human health. for the risk assessment, this study aimed to investigate the concentration and accumulation of total gaseous mercury (tgm) in five typical leafy vegetables (chinese chives (allium tuberosum rottler), amaranth (amaranthus mangostanus l.), rape (brassica campestris l.), lettuce (lactuca sativa l.), and spinach (spinacia oleracea l.)) grown on ... | 2018 | 29043585 |
| amendment of vanadium-contaminated soil with soil conditioners: a study based on pot experiments with canola plants (brassica campestris l.). | we performed pot experiments with canola plants (brassica campestris l.) to evaluate the effect of eight soil conditioners on the amendment of vanadium (v)-contaminated soil based on analysis of the growth of canola plants and the uptake, bioaccumulation, and translocation of heavy metals. tested soil conditioners included polyacrylamide (pam), sepiolite, humic acid (hac), peat, sludge compost (sc), bentonite, lime, and fly ash. results from the analysis of the growth of canola plants and the an ... | 2017 | 29039973 |
| synthesis and biological activity of novel (z)- and (e)-verbenone oxime esters. | twenty-seven (z)- and (e)-verbenone derivatives bearing an oxime ester moiety were designed and synthesized in search of novel bioactive molecules. their structures were confirmed by uv-vis, ftir, nmr, esi-ms, and elemental analysis. the antifungal and herbicidal activities of the target compounds were preliminarily evaluated. as a result, compound (e)-4n (r = β-pyridyl) exhibited excellent antifungal activity with growth inhibition percentages of 92.2%, 80.0% and 76.3% against alternaria solani ... | 2017 | 29023419 |
| black plastic mulch combined with summer cover crop increases the yield and water use efficiency of apple tree on the rainfed loess plateau. | water deficit significantly limits dryland rainfed fruit production, so increasing water conservation is crucial for improving fruit productivity in arid and semiarid areas. in this study, we tested two treatments in an apple orchard: 1) pc treatment comprising black plastic mulch (bpm) (in-row) with weed control (inter-row); 2) and pgc treatment comprising bpm (in-row) combined with a summer cover crop (inter-row) of rape (brassica campestris l.), which was sown in mid-june and was living from ... | 2017 | 28957428 |
| characterization of lignins isolated with alkali from the hydrothermal or dilute-acid pretreated rapeseed straw during bioethanol production. | a better understanding of the lignin in the straw of rapeseed, brassica campestris l., is a prerequisite for promoting the biorefinery industry of rapeseed. two different methods for fractionating lignin from rapeseed straw were proposed in this study. lignin in the raw material was isolated with alkaline solution and recovered by acid precipitation. a comparison between two lignin preparations obtained from two different methods has been made in terms of yield and purity. the structural feature ... | 2018 | 28830775 |
| characterization of a putative pollen-specific arabinogalactan protein gene, bcmf8, from brassica campestris ssp. chinensis. | the bcmf8 (brassica campestris male fertility 8) gene, possessing the features of 'classical' arabinogalactan protein (agp) was isolated from brassica campestris l. ssp. chinensis, makino syn. b. rapa l. ssp. chinensis. this gene was highly abundant in the fertile flower buds but silenced in the sterile ones of genic male sterile a/b line ('zubajh97-01a/b') in b. campestris. expression patterns analysis suggested bcmf8 was a pollen-specific gene, whose transcript started to be expressed at the u ... | 2008 | 17786584 |
| characterization of a novel gene, bcmf7, that is expressed preferentially in pollen of brassica campestris l. ssp. chinensis makino. | pollen formation is important for plant sexual reproduction. to identify the genes that are involved in pollen formation, we performed the genome-wide transcriptional profiling in the flower buds of both male meiotic cytokinesis (mmc) mutant and its wild-type plants of brassica campestris l. ssp. chinensis, syn. b. rapa l. ssp. chinensis. cdna-amplified fragment length polymorphism (cdna-aflp) analysis showed that the mmc mutation resulted in changes in expression of a variety of genes. bcmf7, a ... | 2007 | 17653671 |
| effects of low light on photosynthetic properties, antioxidant enzyme activity, and anthocyanin accumulation in purple pak-choi (brassica campestris ssp. chinensis makino). | anthocyanins are secondary metabolites that contribute to red, blue, and purple colors in plants and are affected by light, but the effects of low light on the physiological responses of purple pak-choi plant leaves are still unclear. in this study, purple pak-choi seedlings were exposed to low light by shading with white gauze and black shading in a phytotron. the responses in terms of photosynthetic properties, carbohydrate metabolism, antioxidant enzyme activity, anthocyanin biosynthetic enzy ... | 2017 | 28609452 |
| in vitro anticancer activity and cytotoxicity screening of phytochemical extracts from selected traditional chinese medicinal plants. | the purpose of this study was to evaluate the anticancer activity of 10 selected traditional chinese medicinal plants on 4 cancer cell lines, namely mcf-7 (breast carcinoma), ball-1 (acute lymphoblastic leukemia), huh-7 (hepatocellular carcinoma), hela (cervical carcinoma) and their cytotoxicity on a normal cell line, namely mcf-10a (non-tumorigenic mammary epithelial line), about which there were few reports previously. | 2017 | 28534383 |
| comparative transcript profiling of fertile and sterile flower buds from multiple-allele-inherited male sterility in chinese cabbage (brassica campestris l. ssp. pekinensis). | we studied the underlying causes of multiple-allele-inherited male sterility in chinese cabbage (brassica campestris l. ssp. pekinensis) by identifying differentially expressed genes (degs) related to pollen sterility between fertile and sterile flower buds. in this work, we verified the stages of sterility microscopically and then performed transcriptome analysis of mrna isolated from fertile and sterile buds using illumina hiseq 2000 platform sequencing. approximately 80% of ~229 million high- ... | 2017 | 28492984 |
| management of textile wastewater for improving growth and yield of field mustard (brassica campestris l.). | disposal of industrial wastewater is a current issue of urbanization. however, this problem can be sorted out by using wastewater as an alternate source of irrigation after the addition of some amendment. in this way, the problem of disposal of wastewater not only will be resolved but also scarcity of irrigation water can be kept off in the future. the current research study was performed to evaluate the influence of different concentrations of wastewater along with canal water for enhancing gro ... | 2017 | 28448168 |
| transcriptome analysis of a female-sterile mutant (fsm) in chinese cabbage (brassica campestris ssp. pekinensis). | female-sterile mutants are ideal materials for studying pistil development in plants. here, we identified a female-sterile mutant fsm in chinese cabbage. this mutant, which exhibited stable inheritance, was derived from chinese cabbage dh line 'ft' using a combination of isolated microspore culture and ethyl methanesulfonate mutagenesis. compared with the wild-type line 'ft,' the fsm plants exhibited pistil abortion, and floral organs were also relatively smaller. genetic analysis indicated that ... | 2017 | 28443127 |
| effects of foliar dressing of selenite and silicate alone or combined with different soil ameliorants on the accumulation of as and cd and antioxidant system in brassica campestris. | this study was conducted to investigate the possibility of using a combined technology to synchronously reduce as and cd accumulation in the edible parts of brassica campestris. the results showed that a foliar application of selenite (se) and silicon (si) combined with soil ameliorants (including ca-mg-p fertilizer, sodium silicate and red mud) showed limited effects on the growth of b. campestris. the as concentration in the leaves of b. campestris in all treatments was below the chinese safet ... | 2017 | 28411516 |
| the genome sequence of brassica campestris chrysovirus 1, a novel putative plant-infecting tripartite chrysovirus. | a putative chrysovirus recovered from brassica campestris var. purpurea and provisionally named "brassica campestris chrysovirus 1" (brccv1) was sequenced. the genome of the putative brccv1 consists of three double-stranded rnas (dsrnas) comprising 3,639 (dsrna 1), 3,567 (dsrna 2) and 3,337 (dsrna 3) base pairs, respectively, each containing a single open reading frame (orf 1-3). the putative proteins encoded by orf 1-3 show homologies to rdrp, cp and chryso-p3 of approved or tentative chrysovir ... | 2017 | 28004250 |