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enantioselective uptake and translocation of a novel chiral neonicotinoid insecticide cycloxaprid in youdonger (brassica campestris subsp. chinensis).for a novel potential commercial chiral pesticide, an independent study on the fate characteristics and residues of each stereoisomer is essential if the application rates for the pesticide and human exposure are to be reduced. the absorption and translocation behavior of a chiral insecticide, cycloxaprid, in plants treated by root immersion and blade smearing was studied using (14)c-labeling tracer techniques. with the root treatment, total absorption of (1r;8s)-cycloxaprid (rs) (12.39%) was mu ...201323873706
analysis of hydroxy fatty acids from the pollen of brassica campestris l. var. oleifera dc. by uplc-ms/ms.ultraperformance liquid chromatography coupled with negative electrospray tandem mass spectrometry (uplc-esi-ms/ms) was used to determine 7 hydroxy fatty acids in the pollen of brassica campestris l. var. oleifera dc. all the investigated hydroxy fatty acids showed strong deprotonated molecular ions [m-h](-), which underwent two major fragment pathways of the allyl scission and the β-fission of the alcoholic hydroxyl group. by comparison of their molecular ions and abundant fragment ions with th ...201326555998
genotypic differences in nitrate uptake, translocation and assimilation of two chinese cabbage cultivars [brassica campestris l. ssp. chinensis (l.)].a hydroponic trial was conducted to investigate genotypic differences in nitrate uptake, translocation and assimilation between low nitrate accumulator l18 and high accumulator h96 of chinese cabbage [brassica campestris l. ssp. chinensis (l.)]. the results suggested that h96 could uptake more nitrate than l18 in the root but lower transport inside leaf cells and assimilation in the leaf. it was showed that root morphology parameters - length, surface area and volume of h96 were 18.0%, 31.6% and ...201323770590
oxygen and air nanobubble water solution promote the growth of plants, fishes, and mice.nanobubbles (<200 nm in diameter) have several unique properties such as long lifetime in liquid owing to its negatively charged surface, and its high gas solubility into the liquid owing to its high internal pressure. they are used in variety of fields including diagnostic aids and drug delivery, while there are no reports assessing their effects on the growth of lives. nanobubbles of air or oxygen gas were generated using a nanobubble aerator (buvitas; ligaric company limited, osaka, japan). b ...201323755221
gc-ms analysis of the extracts from korean cabbage (brassica campestris l. ssp. pekinensis) and its seed.korean cabbage, a member of the brassicaceae family which also includes cauliflower, mustard, radish, and turnip plants, is a crucial leafy vegetable crop. korean cabbage is harvested after completion of the leaf heading process and is often prepared for use in "baechu kimchi", a traditional korean food. many of the components in korean cabbage are essential for proper human nutrition; these components can be divided into two groups: primary metabolites, which include carbohydrates, amino acids, ...201324471136
stereoselective uptake and distribution of the chiral neonicotinoid insecticide, paichongding, in chinese pak choi (brassica campestris ssp. chinenesis).neonicotinoid chiral insecticidal paichongding is a promising substitute for the widely used imidacloprid. four stereoisomers of paichongding, 5r,7r, 5s,7s, 5s,7r and 5r,7s, were employed in both foliage and roots of chinese pak choi to investigate their stereoselective uptake and distribution in pak choi. results showed that after foliar application, no stereoselective absorption into pak-choi plants was observed among the enantiomers. total absorptions were 35.40% of the applied amount for 5r, ...201324140538
commercial versus synthesized polymers for soil erosion control and growth of chinese cabbage.soil erosion leads to environmental degradation and reduces soil productivity. the use of anionic polyacrylamide (pam) and synthesized biopolymer (bp) using lignin, corn starch, acrylamide, and acrylic acid were tested to evaluate soil erosion, water quality, and growth of chinese cabbage (brassica campestris l.). each treatment of pam and bp was applied at 200 kg ha(-1) to loamy sand soil and subjected to a slope of 36% with a 20 mm h(-1) simulated rainfall. application of bp decreased soil ph ...201325674399
indirect prediction of surface ozone concentration by plant growth responses in east asia using mini-open top chambers.we developed small and mobile open top chambers (mini-otc) measuring 0.6 m (w) × 0.6 m (d) × 1.2 m (h) with an air duct of 0.6 m (w) × 0.23 m (d) × 1.2 m (h). the air duct can be filled with activated charcoal to blow charcoal filtered air (cf) into the chamber, as opposed to non-filtered ambient air (nf). ozone sensitive radish raphanus sativus cv. red chime and rosette pakchoi brassica campestris var. rosularis cv. atu171 were exposed to nf and cf in mini-otcs at different locations in east as ...201322752963
the maturation zone is an important target of piriformospora indica in chinese cabbage roots.the mutualistic symbiont piriformospora indica exhibits a great potential in agriculture. the interaction between p. indica and chinese cabbage (brassica campestris cv. chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development. the resulting highly bushy root phenotype of colonized chinese cabbage seedlings differs substantially from reports of other plant species, which prompted the more detailed study of this symbiosis. a large-scale expres ...201324006423
identification of a novel jasmonate-responsive element in the atjmt promoter and its binding protein for atjmt repression.jasmonates (jas) are important regulators of plant biotic and abiotic stress responses and development. atjmt in arabidopsis thaliana and bcntr1 in brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (meja) biosynthesis and are involved in ja signaling. their expression is induced by meja application. to understand its regulatory mechanism, here we define a novel ja-responsive cis-element (jare), g(c)tcctga, in the atjmt and bcntr1 promoters, by ...201323393583
diversity of flora used for the cure of equine diseases in selected peri-urban areas of punjab, pakistan.plants have widely been used and documented for their therapeutic potential in many parts of the world. there are, however, few reports on the use of plants for the treatment of diseases of equines. to this end, participatory epidemiology and rapid rural appraisal techniques were used to document the plants having pharmacotherapeutic significance against different ailments of equines in selected population of punjab, pakistan.201324283263
medicinal uses of honey (quranic medicine) and its bee flora from dera ismail khan district, kpk, pakistan.this study was carried out during 2005-2008 to identify existing plant species visited by workers of honeybees for nectar and pollen collection in dera ismail khan (d.i.khan) district, pakistan. the honeybee species investigated in the area were, rock bee (apis dorsata f.), little bee (a. florea f.) and european honeybee (a. mellifera l.). a detailed list of 86 plant species both wild and cultivated was prepared, out of which 12 species, phulai (acacia modesta wall.), sarsoon (brassica campestri ...201323455201
geographical influences on content of 10-hydroxy-trans-2-decenoic acid in royal jelly in china.the content of 10-hydroxy-trans-2-decenoic acid (10-hda), a marker compound in royal jelly (rj), is the most important criterion in grading rj for commercial trade and varies with its origin. to identify the effect of geographical origin on 10-hda content in rj, 138 samples were collected from 19 provinces of china (divided into three groups) produced by either apis mellifera ligustica spinola, 1806 or a hybrid of a. m. ligustica and apis mellifera carnica pollman, 1879 and analyzed for moisture ...201324224235
bcmf11, a novel non-coding rna gene from brassica campestris, is required for pollen development and male fertility.key message : bcmf11 as a non-coding rna gene has an essential role in pollen development, and might be useful for regulating the pollen fertility of crops by antisense rna technology. we previously identified a 828-bp full-length cdna of bcmf11, a novel pollen-specific non-coding mrna-like gene from chinese cabbage (brassica campestris l. ssp. chinensis makino). however, little information is known about the function of bcmf11 in pollen development. to investigate its exact biological roles in ...201323064614
identification of micrornas potentially involved in male sterility of brassica campestris ssp. chinensis using microrna array and quantitative rt-pcr assays.micrornas (mirnas) are a class of newly identified, noncoding, small rna molecules that negatively regulate gene expression. many mirnas are reportedly involved in plant growth, development and stress response processes. however, their roles in the sexual reproduction mechanisms in flowering plants remain unknown. pollen development is an important process in the life cycle of a flowering plant, and it is closely related to the yield and quality of crop seeds. this study aimed to identify mirnas ...201323864334
the dynamic growth exhibition and accumulation of cadmium of pak choi (brassica campestris l. ssp. chinensis) grown in contaminated soils.the accumulation of heavy metals, especially cadmium (cd), in leafy vegetables was compared with other vegetables. pak choi (brassica campestris l. ssp. chinensis) is a leafy vegetable consumed in taiwan and its safety for consumption after growing in contaminated soils is a public concern. a pot experiment (50 days) was conducted to understand the dynamic accumulation of cd by pak choi grown in artificially contaminated soils. the edible parts of pak choi were sampled and analyzed every 2-3 day ...201324284350
characterization and co-expression analysis of wrky orthologs involved in responses to multiple abiotic stresses in pak-choi (brassica campestris ssp. chinensis).the wrky transcription factor is an important member of the stress-related transcription factors, which mediate diverse abiotic stresses in many plants. however, up until now, the number of wrky members, and the regulatory mechanisms involved in abiotic stress responses in pak-choi (brassica campestris ssp. chinensis), remained unknown.201324267479
comparison of ascorbic acid biosynthesis in different tissues of three non-heading chinese cabbage cultivars.ascorbic acid (l-asa) is an important antioxidant in plants and humans. vegetables are one of the main sources of ascorbic acid for humans. for instance, non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is considered as one of the most important vegetables in south china. to elucidate the mechanism by which asa accumulates, we systematically investigated the expression profiles of d-mannose/l-galactose pathway-related genes. we also investigated the recycling-related genes ...201324157701
identification of gene expression profile during fertilization in brassica campestris subsp. chinensis.fertilization is controlled by a complex gene regulatory network. to study the fertilization mechanism, we determined time courses of the four developmental stages of fertilization in chinese cabbage pak-choi (brassica campestris subsp. chinensis) by cytological observation. we then used the arabidopsis ath1 microarray to characterize the gene expression profiles of pollinated and unpollinated pistils in b. campestris subsp. chinensis. the result showed 44 up-regulated genes and 33 down-regulate ...201323379337
generation and identification of brassica alboglabra-brassica campestris monosomic alien addition lines.four monosomic alien addition lines (maals) for brassica alboglabra-brassica campestris were developed through digenomic triploid (acc) backcrossing with the recurrent parent b. alboglabra (cc). the objectives of this study were to compare morphological traits, microsatellite markers (simple sequence repeats), chromosomal karyotypes, and meiotic behaviors. based on the new chromosome nomenclature system established for brassica, we preliminarily identified these maals as cc+a1, cc+a3, cc+a6, and ...201323659701
microrna expression analysis of rosette and folding leaves in chinese cabbage using high-throughput solexa sequencing.in this study, a global analysis of mirna expression from rosette leaves (rls) and folding leaves (fls) of chinese cabbage (brassica rapa l. ssp. pekinensis) was conducted using high-throughput solexa sequencing. in total, over 12 million clean reads were obtained from each library. sequence analysis identified 64 conserved mirna families in each leaf type and 104 and 95 novel mirnas from rls and fls, respectively. among these, 61 conserved mirnas and 61 novel mirnas were detected in both types ...201324055726
maize grain concentrations and above-ground shoot acquisition of micronutrients as affected by intercropping with turnip, faba bean, chickpea, and soybean.most research on micronutrients in maize has focused on maize grown as a monocrop. the aim of this study was to determine the effects of intercropping on the concentrations of micronutrients in maize grain and their acquisition via the shoot. we conducted field experiments to investigate the effects of intercropping with turnip (brassica campestris l.), faba bean (vicia faba l.), chickpea (cicer arietinum l.), and soybean (glycine max l.) on the iron (fe), manganese (mn), copper (cu) and zinc (z ...201323900569
phytoextraction of zinc, copper, nickel and lead from a contaminated soil by different species of brassica.in a pot culture experiment, five different species of brassica (brassica juncea, brassica campestris, brassica carinata, brassica napus, and brassica nigra) were grown for screening possible accumulators of heavy metals, viz. zn, cu, ni, and pb. the plants were grown to maturity in a soil irrigated with sewage effluents for more than two decades in west delhi, india. the soil analysis showed enhanced accumulation of zn, cu, ni, and pb in this sewage-irrigated soil. among all species, b. carinat ...201318709932
characterisation of cell wall polysaccharides from rapeseed (brassica napus) meal.to enable structural characteristics of individual cell wall polysaccharides from rapeseed (brassica napus) meal (rsm) to be studied, polysaccharide fractions were sequentially extracted. fractions were analysed for their carbohydrate (linkage) composition and polysaccharide structures were also studied by enzymatic fingerprinting. the rsm fractions analysed contained pectic polysaccharides: homogalacturonan in which 60% of the galacturonic acid residues are methyl-esterified, arabinan branched ...201324053853
uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for lowering dehp content of intercropped vegetables.uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for topical phytoremediation were investigated by cultivation of plants in dehp-contaminated environments. the results showed that major plant organs of b. hispida , including leaves, stems, and fruits, readily absorbed dehp from the air. the amount of dehp that accumulated in leaves, stems, and fruits was mainly dependent upon exposure time, and most dehp accumulated in their inner tissues. a single plant of ...201323675826
ethnoveterinary remedies of diseases among milk yielding animals in kathua, jammu and kashmir, india.the triangle of relationship between human beings, animals and plants has existed for ages, and has given rise to intense-relationships and consequently rich traditions of ethnoveterinary knowledge throughout the world. the predominantly rural population and the strong agricultural base have provided unique situation for rich ethnoveterinary practices in the study area.201222366093
effect of different fertilizers on nitrogen isotope composition and nitrate content of brassica campestris.the effect of different fertilizers on the δ(15)n value, nitrate concentration, and nitrate reductase activity of brassica campestris and the δ(15)n value of soil has been investigated through a pot experiment. the δ(15)n mean value of b. campestris at the seedling stage observed in the composted chicken treatment (+8.65‰) was higher than that of chemical fertilizer treatment (+5.73‰), compost-chemical fertilizer (+7.53‰), and control check treatment (+7.86‰). there were significantly different ...201222242784
bcnrt1, a plasma membrane-localized nitrate transporter from non-heading chinese cabbage.a nitrate transporter, bcnrt1, was isolated from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) cultivar 'suzhouqing'. the full-length cdna was obtained using the rapid amplification of cdna ends technique and contains an open reading frame of 1,770 bp that predicts a protein of 589 acid residues that possesses 12 putative transmembrane domains. using the gus marker gene driven by the bcnrt1 promoter, we found bcnrt1 expression to be concentrated in primary and lateral r ...201222539185
sequence characterization and expression pattern of bcmf21, a novel gene related to pollen development in brassica campestris ssp. chinensis.in this report a full length cdna, brassica campestris male fertile 21 (bcmf21) was successfully isolated from one of the cdna-amplified fragment length polymorphism (cdna-aflp) transcript-derived fragments (tdfs), bbp10, that was found down-regulated in the flower buds of sterile plants in brassica campestris l. ssp. chinensis makino genic male sterile (gms) ab line system (bcajh 97-01a/b). bcmf21 protein structure analysis showed a signal peptide at the n-terminus; two protein kinase c phospho ...201222311045
microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading chinese cabbage.microtubules are the basic components of the cytoskeleton in eukaryotic cells and are made up of 13 parallel protofilaments, each composed of α- and β-tubulin unit molecules aligned along the longitudinal axis of the microtubule.201222581783
the role of a dark septate endophytic fungus, veronaeopsis simplex y34, in fusarium disease suppression in chinese cabbage.the soil-inhabiting fungal pathogen fusarium oxysporum has been an increasing threat to chinese cabbage (brassica campestris l.). a dark septate endophytic fungus, veronaeopsis simplex y34, isolated from yaku island, japan, was evaluated in vitro for the ability to suppress fusarium disease. seedlings grown in the presence of the endophyte showed a 71% reduction in fusarium wilt disease and still had good growth. the disease control was achieved through a synergetic effect involving a mechanical ...201222923110
evaluation of rhizospheric pseudomonas and bacillus as biocontrol tool for xanthomonas campestris pv campestris.xanthomonas campestris pv campestris (xcc), causing black rot, is one of the most yield-limiting and destructive pathogens of cruciferous crops. the intention of this study was to evaluate the potential of rhizobacteria in black rot management. fifty-four isolates from rhizosphere soil of brassica campestris were screened against xcc. two isolates namely, ka19 and se, with inhibition radius >11 mm were selected. the combined use of them produced an average inhibition zone of 18.1 ± 1.4 mm radius ...201222806865
[allelopathic effects of streptomyces sp. 6803 on plants].streptomyces can produce an overwhelming majority of known antibiotics and several biologically active compounds, but whether streptomyces can display allelopathic effects on higher plants is largely unknown. in this study, seven actinomyces strains isolated from soils showed inhibitory effect on plant seedlings growth, among which, streptomyces sp. 6803 had strong capability in inhibiting the seedlings growth of brassica campestris and echinochloa crusgalli in both solid and liquid cultures. th ...201223359933
[uptake effect of cd and pb by rape under single cd/pb and cd-pb combined stress].this paper investigated the effects of single cd/pb and cd-pb combined pollution of desert grey soils from the oasis regions on the uptake and translocation of cd and pb by rape (brassica campestris l.), and analyzed the interaction between cd and pb. the results of pot experiment showed that the concentration of cd or pb was promoted when the concentration of cd in soil was less than 7.0 mg x kg(-1) and the concentration of pb in soil was less than 1 500 mg x kg(-1) in single cd/pb and cd-pb co ...201223243889
effects of ambient and elevated level of ozone on brassica campestris l. with special reference to yield and oil quality parameters.tropospheric ozone (o(3)) has become a serious threat to growth and yield of important agricultural crops over asian regions including india. effect of elevated o(3) (ambient+10ppb) was studied on brassica campestris l. (cv. sanjukta and vardan) in open top chambers under natural field conditions. eight hourly mean ambient o(3) concentration varied from 26.3ppb to 69.5ppb during the growth period. plants under o(3) exposure showed reductions in photosynthetic rate, reproductive parameters, yield ...201222986091
[a novel method for extracting leaf-level solar-induced fluorescence of typical crops under cu stress].the leaf-level solar-induced fluorescence changes when the typical crops are under cu stress, which can be considered as a sensitive indicator to estimate the stress level. in the present study, wheat (triticum aestivum l.), pea (pisum sativum l.) and chinese cabbage (brassica campestris l.) were selected and cultured with copper solutions or copper polluted soil with different cu stress. the apparent reflectance of leaves was measured by an asd fieldspec spectrometer and an integrating sphere. ...201222827073
[degradation and bioaccumulation characteristics of ciprofloxacin in soil-vegetable system].a pot experiment was conducted to investigate the effects of applying ciprofloxacin (cip) on the growth of flowering chinese cabbage (brassica campestris), radish (raphanus sativus), tomato (solanum lycopersicum) and long bean (vigna unguiculata), the bioaccumation of cip in the vegetables, and the degradation of the antibiotics in soil. the results showed that applying 5, 30 and 75 mg x kg(-1) of cip had no significant effects on the plant biomass of flowering chinese cabbage, tomato and long b ...201223359930
production and characterization of a somatic hybrid of chinese cabbage and cabbage.in order to broaden chinese cabbage gene pool, we conducted interspecific somatic hybridization between chinese cabbage (brassica campestris, 2n=20, aa) and cabbage (b. oleracea, 2n=18, cc). protoplasts were isolated from 10-day-old cotyledons and hypocotyls of young seedlings, and fused by 40% polyethylene glycol (peg). fused cells were cultured in modified k8p liquid medium supplemented with some plant growth regulators. fusion products were characterized by their morphological, cytological an ...201223289310
synthesis and herbicidal potential of substituted aurones.with the objective of exploring the herbicidal activity of substituted aurones, a series of 4,6-disubstituted and 4,5,6-trisubstituted aurones were synthesised, and their herbicidal activities against brassica campestris l. and echinochloa crusgalli (l.) beauv. were evaluated in laboratory bioassays. effects of some of the compounds were evaluated on seed germination. the most active compounds in the laboratory were evaluated in the greenhouse.201222718431
deciphering the traits associated with pah degradation by a novel serratia marcesencs l-11 strain.polycyclic aromatic hydrocarbons (pahs) are wide spread industrial pollutants that are released into the environment from burning of coal, distillation of wood, operation of gas works, oil refineries, vehicular emission, and combustion process. in this study a lipolytic bacterium was isolated from mixed stover compost of saccharum munja and brassica campestris. this strain was identified by both classical and 16s ribosomal dna sequencing method and designated as serratia marcesencs l-11. hplc-ba ...201222416870
synthesis, structure, and biological activity of novel (oxdi/tri)azoles derivatives containing 1,2,3-thiadiazole or methyl moiety.to develop novel inhibitors of ketol-acid reductoisomerase, a series of (oxdi/tri)azoles derivatives was synthesized and characterized by (1)h  nmr, ms, elemental analyses, and crystallography. according to the biological activities of these compounds obtained both in vivo and in vitro, compound 4-cyclopropyl-3-((4-fluorobenzyl)thio)-5-methyl-4h-1,2,4-triazole showed excellent kari inhibitory activity (100% at 100 μg ml (-1)  in vitro). in addition, most of the title compounds exhibited good her ...201222249419
the pollen wall and tapetum are altered in the cytoplasmic male sterile line rc₇ of chinese cabbage (brassica campestris ssp pekinensis).cytoplasmic male sterile line rc(7) of chinese cabbage produces mature anthers without pollen. to understand the mechanisms involved, we examined the ultrastructural changes during development of the microspores. development of microspores was not affected at the early tetrad stage. during the ring-vacuolated period, some large vacuoles appeared in the tapetum cells, making them larger, extending to the anther sac center during the monocyte period. at the same time, the tapetum degenerated as th ...201223079967
assessment of the interactive effects of ambient o₃ and npk levels on two tropical mustard varieties (brassica campestris l.) using open-top chambers.rising o(3) concentrations in agricultural areas have been identified as a significant threat to crop production in asia including india. the present work reports the results of a field study conducted to assess the usefulness of higher than recommended npk dose in modifying the physiological, growth, yield, and seed quality responses of two mustard (brassica campestris l. var. vardan and aashirwad) varieties under ambient ozone level at a rural site of india, using open-top chambers. twelve hou ...201222072445
phytoextraction of endosulfan a remediation technique.endosulfan is a cyclodiene insecticide used all over the world for the control of various insect pests on variety of food and non crop products. despite judicious use endosulfan has been detected in atmosphere, soil, water, sediment, surface water rain water and food stuffs, which is of concern. in view of the above the use of mustard and maize plants as potential phytoremediation inputs have been evaluated. the potential of mustard (brassica campestris linn.) and maize (zea maize) to remove a o ...201222052137
uptake of human pharmaceuticals and personal care products by cabbage (brassica campestris) from fortified and biosolids-amended soils.human pharmaceuticals and personal care products (ppcps) are routinely found in biosolids from wastewater treatment plants (wwtps). once land applied, the ppcps in biosolids are potentially available for plant uptake and bioaccumulation. this study used a greenhouse model to investigate uptake of ppcps commonly detected in biosolids by the agricultural plant chinese cabbage (brassica campestris). two series of greenhouse experiments were conducted as part of this project. in the first set of exp ...201223051741
chemical fractionations and bioavailability of cadmium and zinc to cole (brassica campestris l.) grown in the multi-metals contaminated oasis soil, northwest of china.a pot experiment was conducted to study the relationship between distribution of cadmium (cd) and zinc (zn) and their availability. to cole (brassica campestris l.) grown in the multi-metal contaminated oasis soil in northwest of china. the results showed that cd and zn in the unpolluted oasis soil was mainly found in the residual fractionation, however, with increasing contents of cd and zn in the oasis soil, the distribution of cd and zn changed significantly. the growth of cole could be promo ...201121517001
production of well-matured compost from night-soil sludge by an extremely short period of thermophilic composting.the effect of various operational conditions on the decomposition of organic material during the composting of night-soil treatment sludge was quantitatively examined. the optimum composting conditions were found to be a temperature of ca. 60°c and an initial ph value of 8. rapid decomposition of organic matter ceased by the sixth day of composting under these optimum conditions, and the final value of the cumulative emission of carbon (e(c)), which represents the degree of organic matter decomp ...201121134735
lead (pb)-inhibited radicle emergence in brassica campestris involves alterations in starch-metabolizing enzymes.lead (pb) is a toxic heavy metal released into the natural environment and known to cause oxidative damage and alter antioxidant mechanism in plants. however, not much is known about the interference of pb with the biochemical processes and carbohydrate metabolism during seed germination. we, therefore, investigated the effect of pb (50-500 μm) upon biochemical alterations in germinating seeds (at 24-h stage) of brassica campestris l. pb treatment significantly enhanced protein and carbohydrate ...201121735112
roles of xanthophylls and exogenous aba in protection against nacl-induced photodamage in rice (oryza sativa l) and cabbage (brassica campestris).changes in actual efficiency of ps ii photochemistry, non-photochemical quenching (npq), content of xanthophylls and kinetics of de-epoxidation were studied in aba-fed and non-aba-fed leaves of rice and cabbage under nacl stress. salt stress induced more progressive decrease in actual efficiency of ps ii photochemistry (фps ii), higher reduction state of ps ii, and a small significant increase in npq in nacl-sensitive rice plants as compared with nacl-tolerant cabbage plants, whereas exogenously ...201121642236
[effects of exogenous dimethylarsinic acid on brassica campestris growth and soil arsenic bioavailability].a pot experiment was conducted to study the effects of exogenous dimethylarsinic acid (dma) on the growth of brassica campestris and the bioavailability of soil arsenic (as). with the increasing concentration of applied dma, the emergence rate and biomass of b. campestris increased at low concentration dma, but decreased at high concentration dma. when the dma concentration reached 90 mg x kg(-1), the emergence rate and biomass of b. campestris in the second cropping decreased by 9.5% and 57.0%, ...201121608259
pedobacter rhizosphaerae sp. nov. and pedobacter soli sp. nov., isolated from the rhizosphere soil of chinese cabbage (brassica campestris).two bacterial strains 01-96(t) and 15-51(t) isolated from the rhizosphere soil of chinese cabbage (brassica campestris), were characterized using a polyphasic approach. the 16s rrna gene sequence analysis showed that strain 01-96(t) was phylogenetically related to pedobacter suwonensis 15-52(t) and pedobacter roseus 25b(t) (96.7 % and 96.7 % sequence similarity, respectively), and strain 15-51(t) was closely related to pedobacter borealis tfb(t) and pedobacter suwonensis 15-52(t) (97.2 % and 97. ...201121257686
1,8-cineole inhibits both proliferation and elongation of by-2 cultured tobacco cells.volatile monoterpenes such as 1,8-cineole inhibit the growth of brassica campestris seedlings in a dose-dependent manner, and the growth-inhibitory effects are more severe for roots than hypocotyls. the preferential inhibition of root growth may be explained if the compounds inhibit cell proliferation more severely than cell elongation because root growth requires both elongation and proliferation of the constituent cells, whereas hypocotyl growth depends exclusively on elongation of existing ce ...201121344180
synthesis, structure and biological activity of novel 1,2,4-triazole mannich bases containing a substituted benzylpiperazine moiety.a series of novel mannich bases with trifluoromethyl-1,2,4-triazole and substituted benzylpiperazine moieties were synthesized. their structures were confirmed by ir, (1) h nmr and elemental analysis. the single crystal structure of compound 4r was also determined. the preliminary bioassays showed that most of the lead compounds had low herbicidal activity against brassica campestris, echinochloa crusgalli, and kari enzyme. however, most of them exhibited significant fungicidal activity at the d ...201121521489
characterization of alginase and elicitor-active oligosaccharides from gracilibacillus a7 in alleviating salt stress for brassica campestris l.alginase was purified from gracilibacillus a7 and evaluated for its ability to produce elicitor-active oligosaccharides. the optimum conditions for the alginase reaction are as follows: temperature, 40 -¦c; ph, 8.0; alginate content, 0.3-0.7%; and the presence of na(+) and mg(2+) metal ions. the degree of polymerization (dp) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. the relative root length (rrl) of the brassic ...201121696216
A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco.WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced q ...201121938429
bioaccumulation and translocation of cadmium in cole (brassica campestris l.) and celery (apium graveolens) grown in the polluted oasis soil, northwest of china.a pot experiment was conducted to study the bioaccumulation and translocation of cadmium (cd) in cole (brassica campestris l.) and celery (apium graveolens) grown in the cd-polluted oasis soil, northwest of china. the results showed that cd in the unpolluted oasis soil was mainly bound to carbonate fraction (f2) and fe-mn oxide fraction (f3). however, marked change of cd fractions was observed with increasing soil cd concentrations, in which the concentration of cd in fl (exchangeable fraction), ...201122128545
influence of various host plants on the consumption and utilization of food by pieris brassicae (linn.).pieris brassicae (linn.) is a destructive cosmopolitan pest of cruciferous crops. it is present wherever its host plants occur, and it is considered to be one of the most widely distributed of all the lepidoptera. we investigated the affect of various host plants on the food consumption and utilization by p. brassicae. we quantified consumption of food, larval duration, pupal duration and weight on cabbage (brassica oleracea var. capitata), cauliflower (brassica oleracea var. botrytis), radish ( ...201122030306
effects of humic acid on phytodegradation of petroleum hydrocarbons in soil simultaneously contaminated with heavy metals.the use of humic acid (ha) to enhance the efficiency of phytodegradation of petroleum hydrocarbons in soil contaminated with diesel fuel was evaluated in this study. a sample of soil was artificially contaminated with commercially available diesel fuel to an initial total petroleum hydrocarbons (tph) concentration of 2300 mg/kg and four heavy metals with concentrations of 400 mg/kg for pb, 200 mg/kg for cu, 12 mg/kg for cd, and 160 mg/kg for ni. three plant species, brassica campestris, festuca ...201122432335
growth promotion of chinese cabbage and arabidopsis by piriformospora indica is not stimulated by mycelium-synthesized auxin.piriformospora indica, an endophytic fungus of the order sebacinales, interacts with the roots of a large variety of plant species. we compared the interaction of this fungus with chinese cabbage (brassica campestris subsp. chinensis) and arabidopsis seedlings. the development of shoots and roots of chinese cabbage seedlings was strongly promoted by p. indica and the fresh weight of the seedlings increased approximately twofold. the strong stimulation of root hair development resulted in a bushy ...201121375386
bcmf20, a putative pollen-specific transcription factor from brassica campestris ssp. chinensis.the arabidopsis transcription factor (at1g26610) was shown to be down-regulated in male sterile lines compared to their maintainer line in chinese cabbage (brassica campestris ssp. chinensis) in our previous study. the bcmf20 gene, homologue of the at1g26610 gene was isolated from chinese cabbage. it encodes a putative c2h2 zinc finger protein. the spatial and temporal expression patterns examined by qrt-pcr and in situ hybridization, indicated bcmf20 is specifically expressed in the developing ...201121327825
isolation and characterization of a novel bcmf14 gene from brassica campestris ssp. chinensis.a putative ralf (rapid alkalinization factor)-like gene (genbank accession number ef523517), named bcmf14, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) by rapid amplification of cdna ends (race) based on a cdna-aflp differential fragment exclusively expressed in fertile line. semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) discovered that bcmf14 was prominently expressed in stage four and five flower buds ...201120857218
a polygalacturonase inhibitory protein gene (bcmf19) expressed during pollen development in chinese cabbage-pak-choi.the level of polygalacturonase inhibitory protein (pgip) genes involved in pollen development remains unclear. characterization of the different pgip genes that are expressed in pollen is necessary in understanding the similarities and differences of functions between the members of this gene family, as well as the underlying mechanism of pollen development. a gene-encoding putative pgip, bcmf19 was successfully cloned on a cdna-amplified fragment length polymorphism fragment after it was found ...201120373034
morphological, cytological, and molecular characterization of hybrids and their progenies derived from the somatic hybridization of brassica campestris and brassica oleracea.in order to produce interspecific somatic hybrids between brassica campestris (2n = 20, aa) and brassica oleracea (2n = 18, cc), we isolated protoplasts from cotyledons and hypocotyls of young seedlings, and fused by 40% polyethylene glycol (peg). fused cells were cultured in modified k8p liquid medium supplemented with 0.2 mg/l 2,4-dichorophenoxyacetic acid (2,4-d) +0.5 mg/l 6-benzylaminopurine (6-ba)+0.1 mg/l naphthaleneacetic acid (naa)+ 0.1 mg/l kinetin (kin), 0.3 mol/l sucrose and 0.3 mol/l ...201122393713
effect of sewage water on mineral nutritive potential of six fodder species grown under semiarid conditions.investigation was performed to assess the effect of different sewage water treatments on the metal status of different fodder species consumed by ruminants under semi-arid conditions. five samples each of six fodder species viz., trifolium alexandrinum, cichorium intybus, avena sativa, medicago polymorpha, brassica campestris and medicago sativa were collected from three fields irrigated with canal water, mix water (canal water and sewage water) and sewage water, respectively. fodder samples wer ...201123961142
swede midge (diptera: cecidomyiidae), ten years of invasion of crucifer crops in north america.the swede midge, contarinia nasturtii kieffer (diptera: cecidomyiidae), a common insect pest in europe, is a newly invasive pest in north america that constitutes a major threat to cruciferous vegetable and field crops. since its first identification in ontario, canada, in 2000, it has rapidly spread to 65 counties in the provinces of ontario and quebec and has recently been found in canola (one of two cultivars of rapeseed, brassica napus l. and brassica campestris l.) in the central prairie re ...201121735885
using matrix solid-phase microextraction (matrix-spme) to estimate bioavailability of ddts in soil to both earthworm and vegetables.this study was conducted to find an appropriate approach for the assessment of bioavailability of ddts in soil to both earthworm and vegetables. four chemical approaches--soxhlet extraction with n-hexane, n-butanol agitation extraction, water agitation extraction, and matrix solid-phase microextraction (matrix-spme)--were used to assess the relationships between the extractability of 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (p,p'-dde), 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) e ...201019418090
pollenopyrroside a and b, novel pyrrole ketohexoside derivatives from bee-collected brassica campestris pollen.pollenopyrroside a and pollenopyrroside b, novel pyrrole ketohexoside derivatives, were isolated from the extract of bee-collected brassica campestris pollen. their structures were elucidated by spectroscopic analysis (uv, ir, ms, nmr and x-ray) and chemical evidence. pollenopyrrosides a and b represent a novel carbon skeleton with a six-six and five-six member dioxaspirocycle bearing nitrogen atom, respectively.201020606352
diffusion and accumulation in cultivated vegetable plants of di-(2-ethylhexyl) phthalate (dehp) from a plastic production factory.di-(2-ethylhexyl) phthalate (dehp) concentrations in the atmosphere and in four vegetable crops including brassica chinensis l. (bok choy), brassica campestris l. (field mustard), vigna unguiculata walp. (cowpea), and solanum melongena l. (eggplant) cultivated on land surrounding a plastic production factory were determined. the air dehp concentrations (means) at the sites 0.2, 0.4, 0.8, and 1.6 km away from the plastic production building were about 9.4-12.8, 5.8-9.6, 1.6-5.0, and 0.04-0.27 mic ...201020496185
soil and vegetables enrichment with heavy metals from geological sources in gilgit, northern pakistan.this study was conducted to investigate the concentrations of heavy metals in soil and vegetables, and human health risks through ingestion of contaminated vegetables. soil and vegetable samples were collected from different locations in gilgit, northern pakistan, and analyzed for cd, cu, ni, pb and zn. plant transfer factors (ptf), daily intake of metals (dim) and health risk index (hri) were also calculated. the concentrations of cd, cu and zn exceeded their respective permissible limits in so ...201020810165
interaction of 8-hydroxyquinoline with soil environment mediates its ecological function.allelopathic functions of plant-released chemicals are often studied through growth bioassays assuming that these chemicals will directly impact plant growth. this overlooks the role of soil factors in mediating allelopathic activities of chemicals, particularly non-volatiles. here we examined the allelopathic potential of 8-hydroxyquinoline (hq), a chemical reported to be exuded from the roots of centaurea diffusa.201020877629
toxicities of fipronil enantiomers to the honeybee apis mellifera l. and enantiomeric compositions of fipronil in honey plant flowers.fipronil is a chiral phenylpyrazole insecticide that is effective for control of a wide range of agricultural and domestic pests at low application rates. wide application of fipronil also causes poisoning of some nontarget insects, such as honeybees. in the present study, toxicities of fipronil enantiomers and racemate to the honeybee apis mellifera l. were determined to examine whether using formulations of single or enriched fipronil enantiomer is a possible option to reduce risks to bees. co ...201020821427
a novel method for constructing pathogen-regulated small rna cdna library.pathogen-responsive endogenous small non-coding rnas regulate gene expression in relation to plant immune responses by serving as rna silencing machinery. decay caused by the bacterium, erwinia carotovora subsp. carotovora (ecc), often leads to soft rot disease in the plant brassica campestris l. ssp. pekinensis (bcp). to discover endogenous small rna species in bcp in response to ecc infection, we developed a highly efficient approach for cloning pathogen-regulated small rnas. a group of degene ...201020515661
ethanolic extracts of brassica campestris spp. rapa roots prevent high-fat diet-induced obesity via beta(3)-adrenergic regulation of white adipocyte lipolytic activity.the influence of ethanolic extracts of brassica campestris spp. rapa roots (ebr) on obesity was examined in imprinting control region (icr) mice fed a high-fat diet (hfd) and in 3t3-l1 adipocytes. the icr mice used were divided into regular diet, hfd, ebr (50 mg/kg/day ebr administered orally), and orlistat (10 mg/kg/day orlistat administered orally) groups. the molecular mechanism of the anti-obesity effect of ebr was investigated in 3t3-l1 adipocytes as well as in hfd-fed icr mice. in the obes ...201020132043
responses of mikania micrantha, an invasive weed to elevated co₂: induction of β-caryophyllene synthase, changes in emission capability and allelopathic potential of β-caryophyllene.to better understand the effect of predicted elevated levels of carbon dioxide (co₂) on an invasive weed mikania micrantha, we constructed a suppressive subtractive hybridization (ssh) library from the leaves of m. micrantha exposed to co₂ at 350 and 750 ppm for 6 d, and isolated a novel gene named β-caryophyllene synthase. β-caryophyllene synthase catalyses the conversion of farnesyl diphosphate to β-caryophyllene, a volatile sesquiterpene with allelopathic potential. real-time pcr analysis rev ...201020821346
role of ca2+ on growth of brassica campestris l. and b. juncea (l.) czern & coss under na+ stress.root and shoot growth of brassica campestris l. and b. juncea increased significantly (p < 0.01) with enhanced ca(2+) treatment along with 60 mm nacl in the root medium. the maximum fresh mass of shoot and root in b. juncea was recorded at 10 mm ca(2+) concentration. the relative growth rate of shoot of both species reached its maximum at 8 mm of ca(2+) concentration. average rate of ca(2+) intake (i(ca)) was higher in b. juncea than b. campestris. in b. juncea, the average transport of ca(2+) t ...201020590985
drying behaviour of rapeseed under thin layer conditions.thin layer drying behaviour of rapeseed (toria, brassica campestris) varieties like 'tl-15', 'th-68' and 'sangam' in the temperature range of 30 to 70°c and at constant air velocity of 2 m/sec was studied. the validity of modified page's equation for prediction of drying time was assessed by fitting the experimental drying data. the drying air temperature affected drying time significantly. the effect of variety on drying time was not significant. drying constants of modified page's model were c ...201023572648
residue analysis of acephate and its metabolite methamidophos in open field and greenhouse pakchoi (brassica campestris l.) by gas chromatography-tandem mass spectrometry.to analyze the dynamic degradation and final residues of acephate and its metabolite methamidophos, field-experiments with pakchoi (brassica campestris l.) in open field and greenhouse were carried out in beijing, china in 2004 and 2005. the degradation dynamics and final residues were determined by gas chromatography (gc) equipped with a pulsed flame photometric detector and gc coupled to mass spectrometry (ms)/ms after acephate was applied on open field and green house pakchoi (b. campestris l ...201019496002
aromatase inhibitory fatty acid derivatives from the pollen of brassica campestris l. var. oleifera dc.two new fatty acid derivatives, 9z,12z,15z-octadecatrienoic acid sorbitol ester (1) and (10,11,12)-trihydroxy-(7z,14z)-heptadecadienoic acid (2), were isolated from the pollen of brassica campestris l. var. oleifera dc., along with the four known fatty acid derivatives, 9z,12z,15z-octadecatrienamide, n-(2-hydroxyethyl) (3), hexadecanoic acid sorbitol ester (4), 15,16-dihydroxy-9z,12z-octadecadienoic acid (5), and 9z,12z,15z-octadecatrienoic acid 2,3-dihydroxypropyl ester (6). their structures we ...200919219725
eco-toxic effects of sulfadiazine sodium, sulfamonomethoxine sodium and enrofloxacin on wheat, chinese cabbage and tomato.investigation of the toxic effects of three veterinary drugs [sulfadiazine sodium (sds), sulfamonomethoxine sodium (smms), and enrofloxacin (eflx)] on seed germination, root elongation and shoot elongation of wheat (triticum aestivum l.), chinese cabbage (brassica campestris l.) and tomato (cyphomandra betacea) was carried out. significant linear relationships between the root and shoot elongation and the concentration of veterinary drugs addition were observed. the effects of the three veterina ...200919554446
evaluation of physiological, growth and yield responses of a tropical oil crop (brassica campestris l. var. kranti) under ambient ozone pollution at varying npk levels.a field study was conducted to evaluate the impact of ambient ozone on mustard (brassica campestris l. var. kranti) plants grown under recommended and 1.5 times recommended npk doses at a rural site of india using filtered (fcs) and non-filtered open top chambers (nfcs). ambient mean o(3) concentration varied from 41.65 to 54.2ppb during the experiment. plants growing in fcs showed higher photosynthetic rate at both npk levels, but higher stomatal conductance only at recommended npk. there were ...200919070410
accumulation and distribution of toxic metals in wheat (triticum aestivum l.) and indian mustard (brassica campestris l.) irrigated with distillery and tannery effluents.in the present study, accumulation and distribution of toxic metals (cu, cd, cr, zn, fe, ni, mn, and pb) and their biochemical effect on wheat and mustard plants irrigated with mixed distillery and tannery effluents are reported. analyses of effluents and soil samples have shown high metal content than the permissible limit except pb. further, analyses of plant samples have indicated the maximum accumulation of fe (340 mg kg(-1) in wheat root and 560 mg kg(-1) in mustard leaves) followed by mn a ...200918650002
over-expression of putative transcriptional coactivator kelp interferes with tomato mosaic virus cell-to-cell movement.tomato mosaic virus (tomv) encodes a movement protein (mp) that is necessary for virus cell-to-cell movement. we have demonstrated previously that kelp, a putative transcriptional coactivator of arabidopsis thaliana, and its orthologue from brassica campestris can bind to tomv mp in vitro. in this study, we examined the effects of the transient over-expression of kelp on tomv infection and the intracellular localization of mp in nicotiana benthamiana, an experimental host of the virus. in co-bom ...200919236566
the lcb2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and bax-induced cell death.previous results showed that expression of the gene encoding the long-chain base2 (lcb(2)) subunit of serine palmitoyltransferase (spt), designated bclcb(2), from nonheading chinese cabbage (brassica campestris ssp. chinensis) was up-regulated during hypersensitive cell death (hcd) induced by the phytophthora boehmeriae elicitor pb90. overexpression of bclcb(2) in nicotiana tabacum leaves suppressed the hcd normally initiated by elicitors and pb90-triggered h(2)o(2) accumulation. bclcb(2) also f ...200919076721
campesin, a thermostable antifungal peptide with highly potent antipathogenic activities.an 9.4-kda antifungal peptide designated as campesin was isolated from seeds of the cabbage brassica campestris. the isolation procedure involved affinity chromatography on affi-gel blue gel, ion exchange chromatography on q-sepharose and mono s, and gel filtration on superdex 75 and superdex peptide. the peptide was adsorbed on the first three chromatographic media. it exerted an inhibitory action on mycelial growth including fusarium oxysporum and mycosphaerella arachidicola, with an ic(50) of ...200919664563
bcmf9, a novel polygalacturonase gene, is required for both brassica campestris intine and exine formation.the polygalacturonase (pg) gene family has been found to be enriched in pollen of several species; however, little is currently known about the function of the pg gene in pollen development. to investigate the exact role that the pg gene has played in pollen development and about this family in general, one putative pg gene, brassica campestris male fertility 9 (bcmf9), was isolated from chinese cabbage (brassica campestris ssp. chinensis, syn. b. rapa ssp. chinensis) and characterized.200919815569
occurrence of clubroot on pak-choi caused by plasmodiophora brassicae.clubroot symptoms occurred severely on roots of pak-choi (brassica campestris ssp. chinensis) grown in greenhouses in gwangju city, gyeonggi province, korea in september, 2008. the incidence of the disease symptoms reached as high as 90% in three greenhouses investigated. the root galls collected from the greenhouses were sectioned using a scalpel and observed by light microscope. many resting spores were found in the cells of the root gall tissues. suspension of resting spores was prepared from ...200923983511
effect of bacillus thuringiensis naturally colonising brassica campestris var. chinensis leaves on neonate larvae of pieris brassicae.the feeding of neonate larvae of pieris brassicae (order lepidoptera) on leaves of brassica plants that had been colonised by bacillus thuringiensis resulted in the death of 35% of the population within 72h. the bacteria multiplied in the cadavers, resulting in an increase of about 50-fold compared to the living insects. surviving insects showed no ill effects during the time of the study. there was negligible multiplication of b. thuringiensis in the frass.200919232351
isolation and characterization of juncin, an antifungal protein from seeds of japanese takana (brassica juncea var. integrifolia).an 18.9 kda antifungal protein designated juncin was isolated from seeds of the japanese takana (brassica juncea var. integrifolia). the purification protocol employed comprised anion-exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, cation exchange chromatography on sp-sepharose, and gel filtration on superdex 75. juncin was adsorbed on affi-gel blue gel and sp-sepharose but unadsorbed on q-sepharose. the protein exhibited antifungal activity toward the phyto ...200919354248
interaction between plants and bacteria: glucosinolates and phyllospheric colonization of cruciferous vegetables by enterobacter radicincitans dsm 16656.for determining interactive plant-bacterial effects between glucosinolates and phyllospheric colonization by a plant growth-promoting strain, enterobacter radicincitans dsm 16656, in cruciferous vegetables, the extent of bacterial colonization was assessed in 5 cruciferous vegetables (brassica juncea, brassica campestris, brassica oleracea var. capitata, brassica rapa var. alboglabra, nasturtium officinale) using a species-specific taqman probe and quantitative real-time pcr. colonization abilit ...200919556746
leaching behaviour of pendimethalin causes toxicity towards different cultivars of brassica juncea and brassica campestris in sandy loam soil.an experiment was conducted at the farm of zonal adaptive research station, uttar banga krishi viswavidhyalaya, pundibari, cooch behar, west bengal to evaluate the effect of pendimethalin on the yield, weed density and phytotoxicity in different varieties of rai (brassica juncea) and yellow sarson (b. campestris var. yellow sarson) under higher soil moisture regime in terai region of west bengal. pre-emergence application of pendimethalin at higher dose i.e. 1.0 kg/ha recorded higher plant morta ...200921217862
screening brassica species for glucosinolate content.glucosinolates (gsls), a group of compounds found in brassica plants, are toxic to some soil-borne plant pathogens because of the toxicity of their hydrolysis products, isothiocyanates. other phytochemicals found in brassica plants, such as phenols and ascorbic acid, may compliment the activity of gsls. a survey of brassica accessions from the national germplasm repository was conducted to identify potential cover crops that could be soil-incorporated for use as biofumigants. ten brassica access ...200919280485
reducing plant uptake of pahs by cationic surfactant-enhanced soil retention.reducing the transfer of contaminants from soils to plants is a promising approach to produce safe agricultural products grown on contaminated soils. in this study, 0-400 mg/kg cetyltrimethylammonium bromide (ctmab) and dodecylpyridinium bromide (ddpb) were separately utilized to enhance the sorption of pahs onto soils, thereby reducing the transfer of pahs from soil to soil solution and subsequently to plants. concentrations of phenanthrene and pyrene in vegetables grown in contaminated soils t ...200919232798
differential expression of salt tolerance related genes in brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee.we examined salt tolerance responsive genes in pak-choi under salt stress and analyze their potential function. the mrna differential display was used to screen the transcript derived fragments (tdfs) related to salinity tolerance in tolerant and moderately tolerant pak-choi germplasm. seventy-eight primer combinations generated 101 differential cdna fragments, which were divided into 10 expression types. seven cdna sequences (genbank accession nos. dq006915~dq006921) obtained and sequenced were ...200919882760
molecular cloning and characterization of a novel pollen predominantly membrane protein gene bcmf12 from brassica campestris ssp. chinensis.a novel membrane protein gene, bcmf12, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends based on a pollen-specific cdna fragment (dn237936). the cdna was 1,155 bp in length with an open reading frame of 894 bp capable of encoding a putative polypeptide of 297 amino acids with an estimated molecular mass of 34.6 kda and a predicted isoelectric point of 9.6. comparative and bioinformatics analyses revealed that bcmf12 showe ...200919169847
the construction of a genetic linkage map of non-heading chinese cabbage (brassica campestris ssp. chinensis makino).non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is one of the most important vegetables in eastern china. a genetic linkage map was constructed using 127 doubled haploid (dh) lines, and the dh population was derived from a commercial hybrid "hanxiao" (lines sw-13 x l-118). out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups(lgs). chi-square tests showed that 42.67% markers were distorted from expected mendelian segregation ratios, and ...200919683673
characterization of a male sterile related gene bcmf15 from brassica campestris ssp. chinensis.data from cdna-aflp analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of brassica campestris l. ssp. chinensis makino, syn. b. rapa l. ssp. chinensis, indicated that mutation of the mmc gene resulted in changes in expression of a variety of genes. a transcript-derived fragment specifically accumulated in the wild-type flower buds was isolated, and the corresponding full-length cdna and dna was subsequentl ...200918034318
morphological and functional characterization of bcmf13 in the antisense-silenced plants of brassica campestris ssp. chinensis var. parachinensis.the gene brassica campestris male fertility 13 (bcmf13, genbank accession number ef158459) was isolated as a reproductive organ-specific gene from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). it is exclusively expressed in stage four and five flower buds of fertile lines and is most strongly expressed in stamens. here, we report a functional characterization of this bcmf13 gene in the antisense-silenced plants. the inflorescence of the bcmf13 mutan ...200918592396
transcriptional differences between the male-sterile mutant bcms and wild-type brassica campestris ssp. chinensis reveal genes related to pollen development.a novel male-sterile mutant which lacks mature pollen, brassisa campestris male sterile (bcms), was identified in brassica campestris l. ssp. chinensis makino (syn. b. rapa ssp. chinensis). genetic analysis revealed that bcms was controlled by a single recessive mutation locus. genome-wide transcriptional profiling was performed on the flower buds of both the bcms mutant and the wild-type from which it originated, and profiling analysis indicated that there were numerous changes in gene expressi ...200818426481
functional analysis of a pollen-expressed polygalacturonase gene bcmf6 in chinese cabbage (brassica campestris l. ssp. chinensis makino).in our earlier work, a pollen-expressed polygalacturonase gene bcmf6 was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino) by cdna-amplified fragment length polymorphism (cdna-aflp) transcript profiling and rapid amplification of cdna ends (race). to unravel the biological function of bcmf6 gene, the antisense fragment from the bcmf6 gene with a9 promoter and camv35s promoter was transferred into flowering chinese cabbage (b. campestris ssp. chinensis var ...200818415101
characterization and functional analysis of a novel pcp gene bcmf5 from chinese cabbage (brassica campestris l. ssp. chinensis makino).brassica campestris male fertile 5 (bcmf5), a novel member of the pollen coat protein class a (pcp-a) gene family, was identified from brassica campestris l. ssp. chinensis makino (chinese cabbage-pak-choi). temporal and spatial expression analysis showed that bcmf5 is a late-expressed pcp gene related to the process of determining pollen fertility. functional analysis by hairpin rna (hprna)-mediated rna interference also showed that the expression of bcmf5 is inhibited, which resulted in the lo ...200817945379
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