point mutations in cauliflower mosaic virus gene vi confer host-specific symptom changes.mutants of cauliflower mosaic virus (camv) strain d4 have been characterized with regard to host-specific phenotypes that resulted from specific changes in the viral dna sequence. both the mutant and the wild-type viruses infect a brassicaceous host, brassica campestris, systemically, giving indistinguishable symptoms. however, in the solanaceous host datura stramonium, which was systemically infectible by the wild-type virus, mutants induced necrotic local lesions at 21 degrees c and above, and ...20072134858
the use of lacz marker in enumeration of azotobacter chroococcum in carrier based inoculants.a transconjugant of azotobacter chroococcum mac 27 tagged with lac z(a. chroococcum mac27 l) was found to possess high levels of β-galactosidase activity constitutively. further, the lac z marker was found to be stably integrated into the chromosome of the a. chroococcum mac 27 and did not have any adverse effect on growth, nitrogen fixation and excretion of ammonia. a quick method to determine the viable cell number in broth culture and carrier based inoculants has been developed on the basis o ...201425242946
interaction of 8-hydroxyquinoline with soil environment mediates its ecological function.allelopathic functions of plant-released chemicals are often studied through growth bioassays assuming that these chemicals will directly impact plant growth. this overlooks the role of soil factors in mediating allelopathic activities of chemicals, particularly non-volatiles. here we examined the allelopathic potential of 8-hydroxyquinoline (hq), a chemical reported to be exuded from the roots of centaurea diffusa.201020877629
the polygalacturonase gene bcmf2 from brassica campestris is associated with intine development.brassica campestris male fertility 2 (bcmf2) is a putative polygalacturonase (pg) gene previously isolated from the flower bud of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). this gene was found to be expressed specifically in tapetum and pollen after the tetrad stage of anther development. antisense rna technology was used to study the function of bcmf2 in chinese cabbage. scanning and transmission electron microscopy revealed that there were defo ...200819039102
bcmf9, a novel polygalacturonase gene, is required for both brassica campestris intine and exine formation.the polygalacturonase (pg) gene family has been found to be enriched in pollen of several species; however, little is currently known about the function of the pg gene in pollen development. to investigate the exact role that the pg gene has played in pollen development and about this family in general, one putative pg gene, brassica campestris male fertility 9 (bcmf9), was isolated from chinese cabbage (brassica campestris ssp. chinensis, syn. b. rapa ssp. chinensis) and characterized.200919815569
bcpmi2, isolated from non-heading chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco.phosphomannose isomerase (pmi) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (asa) biosynthesis in plants. to clarify the physiological roles of pmi in asa biosynthesis, the cdna sequence of pmi was cloned from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) and overexpressed in tobacco transformed with agrobacterium tumefaciens. the asa and soluble sugar contents were lower in 35s::bcpmi2 tobacco than in wild-type tobacco. howeve ...201424430300
agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor').a procedure for the regeneration of fertile transgenic chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor') is presented in this report. the protocol is based on infection of cotyledon explants of 5-d-old seedlings with an agrobacterium tumefaciens strain lba4404 carrying a disarmed binary vector ptok/bks-1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower m ...199524194308
the effects of acetosyringone and ph on agrobacterium-mediated transformation vary according to plant species.expiants of five plant species (allium cepa, antirrhinum majus, brassica campestris. glycine max, and nicotiana tabacum) were co-cultivated with three agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium ph on strain virulence. tumours were incited on all dicotyledonous species by strains n2/73 and a281. the presence of acetosyringone during co-cultivation generally enhanced the virulence of these strains, most markedly n2/73 on a. majus ...199124213690
the endo-β-1,4-glucanase of bacillus amyloliquefaciens is required for optimum endophytic colonization of plants.the egls gene in bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. in this study, a disruption mutant of gene egls was constructed to examine its role in bacterial adaptation in plants. the mutant tb2k, egls gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. when inoculated on brassica campestris, the tb2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, ...201626907762
diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens.the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ...201627095454
evaluation of rhizospheric pseudomonas and bacillus as biocontrol tool for xanthomonas campestris pv campestris.xanthomonas campestris pv campestris (xcc), causing black rot, is one of the most yield-limiting and destructive pathogens of cruciferous crops. the intention of this study was to evaluate the potential of rhizobacteria in black rot management. fifty-four isolates from rhizosphere soil of brassica campestris were screened against xcc. two isolates namely, ka19 and se, with inhibition radius >11 mm were selected. the combined use of them produced an average inhibition zone of 18.1 ± 1.4 mm radius ...201222806865
genome sequence of a recombinant brassica yellows virus infecting chinese cabbage.rna from a chinese cabbage plant (brassica campestris ssp. pekinensis) showing leaf malformation and mottling was labeled and hybridized to a dna chip capable of detecting plant viruses and viroids. probes specific for beet mild yellowing virus (bmyv) and beet western yellows virus (bwyv) yielded positive results, suggesting that the plant was infected by a polerovirus. primers designed from the sequences of the positive probes were used to amplify and sequence one portion of the viral genome. t ...201525352211
the function of the flowering time gene agl20 is conserved in crucifers.the mads box gene, agamous-like 20 (agl20), integrates environmental and endogenous flowering signals in arabidopsis thaliana. in order to determine if its role is conserved in other plants, we isolated agl20 orthologs from brassica campestris, cardamine flexuosa and draba nemorosa. the putative amino acid sequences of the orthologs were 94 to 97% identical. we analyzed the flowering phenotype and expression level of the agl20 ortholog in c. flexuosa, a long day plant that does not respond to ve ...200314503858
detection of cleavage products from an in vivo transcribed cis hairpin ribozyme in turnips using the camv plant order to examine ribozyme (rz) activity in vivo, we have adapted a virus to deliver rz to plants. dna fragments that code for both active and mutant cis-hairpin rz were cloned into the double-stranded dna plant virus, cauliflower mosaic virus (camv). these rz constructs successfully infected brassica campestris rapa (turnip). the plants that were infected with the active-rz construct showed, on average, a one-week delay in the appearance of viral symptoms, when compared to the mutant-rz contr ...19957622041
the mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains.we inoculated the leaves of turnip plants (brassica campestris spp. rapa cv. just right) with two cauliflower mosaic viruses (camvs) with different small mutations in a dispensable region of the viral genome, and followed the spread of the virus infection through the plant. surprisingly, analysis of viral dna in single primary chlorotic lesions revealed the presence of both mutants. in contrast, the secondary chlorotic lesions and systemically infected leaves contained virus molecules of either ...19921607862
genetic analysis of determinants of disease severity and virus concentration in cauliflower mosaic virus.cauliflower mosaic virus (camv) strains cm1841 and w260 produced markedly different symptoms when inoculated onto turnips (brassica campestris l. 'just right'). the cm1841 strain induced a mild degree of stunting of infected plants while strain w260 caused moderate to severe stunting. although cm1841 was significantly milder than w260, it accumulated to a significantly higher concentration than w260 in systemically infected leaves. we constructed a series of hybrid viruses in order to map region ...19912014640
expression of mammalian metallothionein suppresses glucosinolate synthesis in brassica campestris.transfection of brassica campestris leaves with cauliflower mosaic virus (camv) harboring a mammalian metallothionein (mt) cdna at the orfii position lowered the glucosinolate (gs) concentration to approximately one-half the level in leaves infected with wild-type camv. this suppression was independent of the plant's sulfate status, suggesting that the pathways for protein (mt) and gs biosynthesis were competing for s on an equal basis. the expression of mt may have lowered the endogenous levels ...199016667497
genetic transformation of brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome.a hybrid cauliflower mosaic virus (camv) genome containing a selectable marker gene was constructed by replacing the gene vi coding region with the aminoglycoside (neomycin) phosphotransferase type ii [aph(3')ii] gene from tn5. this modified viral genome was tested for its infectivity both in planta and in a protoplast transformation system of brassica campestris var. rapa. stable, genetically transformed cell lines of b. campestris var. rapa were obtained after transformation. dna of the hybrid ...198624307380
the role of a dark septate endophytic fungus, veronaeopsis simplex y34, in fusarium disease suppression in chinese cabbage.the soil-inhabiting fungal pathogen fusarium oxysporum has been an increasing threat to chinese cabbage (brassica campestris l.). a dark septate endophytic fungus, veronaeopsis simplex y34, isolated from yaku island, japan, was evaluated in vitro for the ability to suppress fusarium disease. seedlings grown in the presence of the endophyte showed a 71% reduction in fusarium wilt disease and still had good growth. the disease control was achieved through a synergetic effect involving a mechanical ...201222923110
[identification of soft rot pathogens on chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing].this study aimed to identify soft rot pathogens of chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing.201526939453
occurrence of clubroot on pak-choi caused by plasmodiophora brassicae.clubroot symptoms occurred severely on roots of pak-choi (brassica campestris ssp. chinensis) grown in greenhouses in gwangju city, gyeonggi province, korea in september, 2008. the incidence of the disease symptoms reached as high as 90% in three greenhouses investigated. the root galls collected from the greenhouses were sectioned using a scalpel and observed by light microscope. many resting spores were found in the cells of the root gall tissues. suspension of resting spores was prepared from ...200923983511
the occurrence of free and bound cytokinins in plasmodia of plasmodiophora brassicae isolated from tissue cultures of clubroots.plasmodia have been isolated from brassica campestris tissue infected with plasmodiophora brassicae. cytokinins were extracted from plasmodia and from the remaining host cytoplasm. fractions were separated in a butanol soluble fraction containing free cytokinins and a water soluble fraction containing bound forms, presumably glucose-6-phosphate derivatives of zeatin and zeatin riboside. the butanol fraction was analysed by high pressure liquid chromatography (hplc). the different glucosides were ...198124258747
basic helix-loop-helix transcription factor bcbhlhpol functions as a positive regulator of pollen development in non-heading chinese cabbage.cytoplasmic male sterility (cms) is a common trait in higher plants, and several transcription factors regulate pollen development. previously, we obtained a basic helix-loop-helix transcription factor, bcbhlhpol, via suppression subtractive hybridization in non-heading chinese cabbage. however, the regulatory function of bcbhlhpol during anther and pollen development remains unclear. in this study, bcbhlhpol was cloned, and its tissue-specific expression profile was analyzed. the results of rea ...201425147023
identification of a novel jasmonate-responsive element in the atjmt promoter and its binding protein for atjmt repression.jasmonates (jas) are important regulators of plant biotic and abiotic stress responses and development. atjmt in arabidopsis thaliana and bcntr1 in brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (meja) biosynthesis and are involved in ja signaling. their expression is induced by meja application. to understand its regulatory mechanism, here we define a novel ja-responsive cis-element (jare), g(c)tcctga, in the atjmt and bcntr1 promoters, by ...201323393583
effects of temperature on systemic infection and symptom expression of turnip mosaic virus in chinese cabbage (brassica campestris).using the chinese cabbage (brassica campestris) cultivar 'chun-goang' as a host and turnip mosaic virus (tumv) as a pathogen, we studied the effects of ambient temperature (13°c, 18°c, 23°c, 28°c and 33°c) on disease intensity and the speed of systemic infection. the optimal temperature for symptom expression of tumv was 18-28°c. however, symptoms of viral infection were initiated at 23-28°c and 6 days post infection (dpi). plants maintained at 33°c were systemically infected as early as 6 dpi a ...201526673094
genome-wide identification of turnip mosaic virus-responsive micrornas in non-heading chinese cabbage by high-throughput sequencing.turnip mosaic virus (tumv) is the most prevalent viral pathogen infecting most cruciferous plants. micrornas (mirnas) are around 22 nucleotides long non-protein-coding rnas that play key regulatory roles in plants. recent research findings show that mirnas are involved in plant-virus interaction. however we know little about plant defense and viral offense system networks throughout microrna regulation pathway. in this study, two small rna libraries were constructed based on non-heading chinese ...201526115771
gene expression in brassica campestris showing a hypersensitive response to the incompatible pathogen xanthomonas campestris pv. vitians.xanthomonas campestris pv. vitians, a pathogen of lettuce, elicits a hypersensitive response within 12 hours of inoculation into brassica leaves, characterized by tissue collapse, loss of membrane integrity, vein blockage and melanin production. in contrast, the compatible pathogen, x. c. pv. campestris, has no visible effects on leaves for 48 hours, after which inoculated areas show chlorosis which eventually spreads, followed by rotting.mrna was prepared from leaves inoculated with suspensions ...198724301262
assessment of heavy metal pollution in vegetables and relationships with soil heavy metal distribution in zhejiang province, china.there are increasing concerns on heavy metal contaminant in soils and vegetables. in this study, we investigated heavy metal pollution in vegetables and the corresponding soils in the main vegetable production regions of zhejiang province, china. a total of 97 vegetable samples and 202 agricultural soil samples were analyzed for the concentrations of cd, pb, as, hg, and cr. the average levels of cd, pb, and cr in vegetable samples [chinese cabbage (brassica campestris spp. pekinensis), pakchoi ( ...201526013654
linkage mapping of genes controlling resistance to white rust (albugo candida) in brassica rapa (syn. campestris) and comparative mapping to brassica napus and arabidopsis thaliana.genes for resistance to white rust (albugo candida) in oilseed brassica rapa were mapped using a recombinant inbred (ri) population and a genetic linkage map consisting of 144 restriction fragment length polymorphism (rflp) markers and 3 phenotypic markers. young seedlings were evaluated by inoculating cotyledons with a. candida race 2 (ac2) and race 7 (ac7) and scoring the interaction phenotype (ip) on a 0-9 scale. the ip of each line was nearly identical for the two races and the population sh ...200211908664
synthesis and biological evaluations of a series of thaxtomin analogues.thaxtomins are a unique family of phytotoxins with unique 4-nitroindole and diketopiperazine fragments possessing potential herbicidal activities. this work presents the total synthesis of natural product thaxtomin c and its analogues. the extensive structure-activity relationship study screens four effective compounds, including thaxtomin a and thaxtomin c. it is indicated that 4-nitro indole fragment is essential for phytotoxicity, while benzyl and m-hydroxybenzyl substituents on the diketopip ...201525804187
ethnoveterinary plants for the treatment of camels in shiwalik regions of kathua district of jammu & kashmir, india.camel is an important mode of transportation in the hot and hilly tracts of shiwaliks of kathua districts. the camel owners of the region lack the modern veterinary facilities and therefore depend heavily upon local treatments for the animal. this ethnoveterinary knowledge of plants is acquired by them from their forefathers and generally moves from one generation to another orally. the oral mode of transferring this valuable knowledge is vulnerable to erosion with the passage of time and genera ...201525917839
diversity of flora used for the cure of equine diseases in selected peri-urban areas of punjab, pakistan.plants have widely been used and documented for their therapeutic potential in many parts of the world. there are, however, few reports on the use of plants for the treatment of diseases of equines. to this end, participatory epidemiology and rapid rural appraisal techniques were used to document the plants having pharmacotherapeutic significance against different ailments of equines in selected population of punjab, pakistan.201324283263
discovery of (2-benzoylethen-1-ol)-containing 1,2-benzothiazine derivatives as novel 4-hydroxyphenylpyruvate dioxygenase (hppd) inhibiting-based herbicide lead compounds.a series of (2-benzoylethen-1-ol)-containing benzothiazine derivatives was synthesized, and their herbicidal activities were first evaluated. the bioassay results indicated that some of 3-benzoyl-4-hydroxy-2-methyl-2h-1,2-benzothiazine-1,1-dioxide derivatives displayed good herbicidal activity in greenhouse testing, especially, compound 4w had good pre-emergent herbicidal activities against brassica campestris, amaranthus retroflexus and echinochloa crusgalli even at a dosage of 187.5 g ha(-1). ...201626682702
pyrimidinyl-substituted amides and thioureas: syntheses, crystal structure and herbicidal activities.the high herbicidal activities of [1,2,4]triazolo[1,5-c]pyrimidine and 2h-1,2,4-thiadiazolo[2,3-a]pyrimidine derivatives suggested the development of new fused heterocyclic compounds for application as herbicides.200818080290
medicinal uses of honey (quranic medicine) and its bee flora from dera ismail khan district, kpk, pakistan.this study was carried out during 2005-2008 to identify existing plant species visited by workers of honeybees for nectar and pollen collection in dera ismail khan (d.i.khan) district, pakistan. the honeybee species investigated in the area were, rock bee (apis dorsata f.), little bee (a. florea f.) and european honeybee (a. mellifera l.). a detailed list of 86 plant species both wild and cultivated was prepared, out of which 12 species, phulai (acacia modesta wall.), sarsoon (brassica campestri ...201323455201
antioxidant enzyme activities and lipid oxidation in rape (brassica campestris l.) bee pollen added to salami during processing.the present research investigated the antioxidant effect of rape (brassica campestris l.) bee pollen (rbp) on salami during processing. eight flavonoids in rbp ethanol extract were quantified by high-performance liquid chromatography-mass spectrometry (hplc-ms) analysis, and quercetin, rutin, and kaempferol were the major bioactive compounds. the rbp ethanol extract exhibited higher total antioxidant capacity than 6-hydroxy-2,5,7,8-tertramethylchromancarboxylic acid (trolox) at the same concentr ...201627801831
identification of dietetically absorbed rapeseed (brassica campestris l.) bee pollen micrornas in serum of mice.micrornas (mirnas) are a class of small noncoding rna that, through mediating posttranscriptional gene regulation, play a critical role in nearly all biological processes. over the last decade it has become apparent that plant mirnas may serve as a novel functional component of food with therapeutic effects including anti-influenza and antitumor. rapeseed bee pollen has good properties in enhancing immune function as well as preventing and treating disease. in this study, we identified the exoge ...201627597967
soxhlet-assisted matrix solid phase dispersion to extract flavonoids from rape (brassica campestris) bee pollen.soxhlet-assisted matrix solid phase dispersion (sa-mspd) method was developed to extract flavonoids from rape (brassica campestris) bee pollen. extraction parameters including the extraction solvent, the extraction time, and the solid support conditions were investigated and optimized. the best extraction yields were obtained using ethanol as the extraction solvent, silica gel as the solid support with 1:2 samples to solid support ratio, and the extraction time of one hour. comparing with the co ...201526454344
geographical influences on content of 10-hydroxy-trans-2-decenoic acid in royal jelly in china.the content of 10-hydroxy-trans-2-decenoic acid (10-hda), a marker compound in royal jelly (rj), is the most important criterion in grading rj for commercial trade and varies with its origin. to identify the effect of geographical origin on 10-hda content in rj, 138 samples were collected from 19 provinces of china (divided into three groups) produced by either apis mellifera ligustica spinola, 1806 or a hybrid of a. m. ligustica and apis mellifera carnica pollman, 1879 and analyzed for moisture ...201324224235
pollenopyrroside a and b, novel pyrrole ketohexoside derivatives from bee-collected brassica campestris pollen.pollenopyrroside a and pollenopyrroside b, novel pyrrole ketohexoside derivatives, were isolated from the extract of bee-collected brassica campestris pollen. their structures were elucidated by spectroscopic analysis (uv, ir, ms, nmr and x-ray) and chemical evidence. pollenopyrrosides a and b represent a novel carbon skeleton with a six-six and five-six member dioxaspirocycle bearing nitrogen atom, respectively.201020606352
overexpression of a brassica campestris hsp70 in tobacco confers enhanced tolerance to heat stress.heat shock proteins (hsps) exist extensively in eukaryotes and are conserved molecular chaperones with important contribution to plant's survival under environmental stresses. here, the cloning and characterization of one complementary dna (cdna) designated as bchsp70 from young seedlings of brassica campestris were reported in the present work. bioinformatic analysis revealed that bchsp70 belongs to the plant hsp gene family and had the closest relationship with hsp70-4 from arabidopsis thalian ...201626298102
identification and expression analysis of bomf25, a novel polygalacturonase gene involved in pollen development of brassica oleracea.bomf25 acts on pollen wall. polygalacturonase (pg) is a pectin-digesting enzyme involved in numerous plant developmental processes and is described to be of critical importance for pollen wall development. in the present study, a pg gene, bomf25, was isolated from brassica oleracea. bomf25 is the homologous gene of at4g35670, a pg gene in arabidopsis thaliana with a high expression level at the tricellular pollen stage. collinear analysis revealed that the orthologous gene of bomf25 in brassica ...201525967087
molecular characterization of two glutathione peroxidase genes of panax ginseng and their expression analysis against environmental stresses.glutathione peroxidases (gpxs) are a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ros). gpx catalyzes the reduction of hydrogen peroxide (h2o2) or organic hydroperoxides to water or alcohols by reduced glutathione. the presence of gpxs in plants has been reported by several groups, but the roles of individual members of this family in a single plant species have not been studied. two gpx cdnas were isolated and characterized from the embryoge ...201424269671
[molecular cloning and characterization of bcmybogu, a novel member of the myb family involved in ogucms in brassica campestris ssp. chinensis].in the attempt to elucidate the molecular mechanism of cms. ogura cytoplasmic male sterile (ogucms) lines were obtained in chinese cabbage after interspecific hybridization between brassica. napus l. ogucms and b. campestris ssp. chinensis followed by recurrent backcross with b. campestris ssp. chinensis as the pollen donor. the cms lines were significantly characterized by the whitish anther and indehiscence of anther. the tapetal hypertrophy with excess vacuola-tion was the first observed defe ...200717548334
a rac-like small g-protein from brassica campestris activates a pkc-dependent phospholipase d.a cdna clone encoding a rac-like small gtp binding protein was isolated from a cdna library of chinese cabbage (brassica campestris l. ssp. pekinensis) flower buds and named brac1. the brac1 cdna contains an open reading frame encoding 198 amino acid residues with an estimated molecular mass of 21,690 da and this coding region has conserved residues and motifs unique to the rho subfamily of proteins. the deduced amino acid sequence of the brac1 protein is closely related to that of arabidopsis t ...200414697272
ntr1 encodes a floral nectary-specific gene in brassica campestris l. ssp. pekinensis.we have characterized a gene specifically expressed in the floral nectaries of brassica campestris l. ssp. pekinensis. differential screening led to the isolation of a floral nectary-specific cdna clone. northern hybridization indicated that its mrna transcript is 1450 nucleotides long and specific to the flower base. in situ hybridization and immunolocalization showed that its mrna and protein are localized specifically to both the lateral and median nectaries of flowers. the cdna codes for a 4 ...200010809010
a high frequency of intergenomic mitochondrial recombination and an overall biased segregation of b. campestris or recombined b. campestris mitochondria were found in somatic hybrids made within brassicaceae.mitochondrial segregation and rearrangements were studied in regenerated somatic hybrids from seven different species combinations produced using reproducible and uniform methods. the interspecific hybridizations were made between closely or more distantly related species within the brassicaceae and were exemplified by three intrageneric, two intergeneric and two intertribal species combinations. the intrageneric combinations were represented by brassica campestris (+) b. oleracea, b. napus (+) ...199424190472
"arabidobrassica": chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells.a somatic hybrid cell line, cloned from an individual protoplast-fusion product between arabidopsis thaliana and brassica campestris, gave rise to formation of numerous plants differing drastically in morphology. analysis of these various regenerants, all of which originated from one and the same heterokaryon derived from the fusion of two cells, shows the unspecific elimination of chromosomes of both parental species during the callus growth phase. whereas the parental cells have so far not bee ...198124275880
fraction i protein analysis of parasexual hybrid plants arabidopsis thaliana + brassica campestris.the polypeptide composition of fraction i protein (ribulose-1,5-bisphosphate carboxylase) prepared from leaves of two clones of the parasexual hybrid plant arabidopsis thaliana + brassica campestris as well as their parents was analyzed by isoelectric focusing. the protein in hybrid plants contained a heterogenous population of small subunits resulting from the expression of both arbabidopsis and brassica nuclear genes, whereas the large subunit polypeptides, and hence the functional chloroplast ...198124258862
"arabidobrassica": a novel plant obtained by protoplast fusion.using somatic hybrid cell lines arabidopsis thaliana+brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. most regenerants show morphological ...198024306240
"arabidobrassica": evidence for intergeneric somatic hybrid nature from electron microscopic morphometry of chromatin.the new method of species and hybrid characterization and determination by quantitative morphometry of chromatin texture in ultra-thin sections of nuclei was applied to the somatic hybrid "arabidobrassica" and its parents, arabidopsis thaliana and brassica campestris. the somatic hybrid exhibits the average amount of condensed chromatin, if compared to the proportion of condensed chromatin in nuclei of the parent species. this result corresponds to similar findings in natural allopolyploids and ...19807398664
a ceramide and cerebroside from the starfish asterias amurensis lütken and their plant-growth promotion activities.the new phytosphingosine-type ceramide asteriaceramide a (1) and glucocerebroside asteriacerebroside g (2), together with two known cerebrosides, asteriacerebrosides a and b, were isolated from lipophilic fractions of the whole bodies of the northern pacific starfish asterias amurensis lütken. the water-soluble fraction afforded two known asterosaponins, glycoside b(2) and asterosaponin-1. the structures of 1 and 2 were determined on the basis of chemical and spectroscopic evidence as (2s,3s,4r, ...200616872149
effect of sewage water on mineral nutritive potential of six fodder species grown under semiarid conditions.investigation was performed to assess the effect of different sewage water treatments on the metal status of different fodder species consumed by ruminants under semi-arid conditions. five samples each of six fodder species viz., trifolium alexandrinum, cichorium intybus, avena sativa, medicago polymorpha, brassica campestris and medicago sativa were collected from three fields irrigated with canal water, mix water (canal water and sewage water) and sewage water, respectively. fodder samples wer ...201123961142
cadmium phytotoxicity: quantitative sensitivity relationships between classical endpoints and antioxidative enzyme this work, cadmium phytotoxicity and quantitative sensitivity relationships between different hierarchical endpoints in plants cultivated in a contaminated soil were studied. thus, germination rate, biomass growth and antioxidative enzyme activity (i.e. superoxide dismutase, peroxidase, catalase and glutathione reductase) in three terrestrial plants (avena sativa l., brassica campestris l. cv. chinensis, lactuca sativa l. cv. hanson) were analyzed. plant growth tests were carried out accordin ...200615982719
damage potential of grasshoppers (orthoptera: acrididae) on early growth stages of small-grains and canola under subarctic conditions.we characterized the type and extent of grasshopper injury to above- and below-ground plant parts for four crops [barley (hordeum vulgare l.), oats (avena sativa l.), wheat (triticum aestivum l.), and canola (brassica campestris l.)] commonly grown, or with potential to grow, in central alaska. cages were placed on 48 pots containing plants in second to third leaf stages and stocked with 0, 2, 4, and 6 first-instar melanoplus sanguinipes f. pot(-1). plants were harvested 22 d after planting. ste ...200314503591
classical and biochemical endpoints in the evaluation of phytotoxic effects caused by the herbicide trichloroacetate.three terrestrial plant species, oat (avena sativa ), chinese cabbage (brassica campestris cv. chinensis) and lettuce (lactuca sativa), were exposed to different concentrations of herbicide tca (sodium trichloroacetate) in a growth test according to guideline oecd # 208. classical (i.e. germination and biomass) and biochemical (i.e., antioxydant enzyme activities) endpoints were investigated. germination rate decreased significantly at 3.9 mg tca kg dry soil(-1) (for oat and lettuce) and 62.5 mg ...200011064042
ethnoveterinary remedies of diseases among milk yielding animals in kathua, jammu and kashmir, india.the triangle of relationship between human beings, animals and plants has existed for ages, and has given rise to intense-relationships and consequently rich traditions of ethnoveterinary knowledge throughout the world. the predominantly rural population and the strong agricultural base have provided unique situation for rich ethnoveterinary practices in the study area.201222366093
heavy metals bioconcentration from soil to vegetables and assessment of health risk caused by their ingestion.the present study was undertaken to assess the non-carcinogenic human health risk of heavy metals through the ingestion of locally grown and commonly used vegetables viz. raphanus sativus (root vegetable), daucus carota (root vegetable), benincasa hispida (fruit vegetable) and brassica campestris leaves (leafy vegetable) in a semi-urbanized area of haryana state, india. heavy metal quantification of soil and vegetable samples was done using flame atomic absorption spectrophotometer. lead, cadmiu ...201424464601
uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for lowering dehp content of intercropped vegetables.uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for topical phytoremediation were investigated by cultivation of plants in dehp-contaminated environments. the results showed that major plant organs of b. hispida , including leaves, stems, and fruits, readily absorbed dehp from the air. the amount of dehp that accumulated in leaves, stems, and fruits was mainly dependent upon exposure time, and most dehp accumulated in their inner tissues. a single plant of ...201323675826
determination of three triterpene alcohols in rat plasma after oral administration of pollen of brassica campestris based on the utilization of fetal bovine serum as surrogate matrix.24-dehydropollinstanol (deh), 24-methylene cholesterol (met) and 31-norcycloartenol (nor) are the functional triterpene alcohols of pollen of brassica campestris. to study the pharmacokinetics of the above components of pollen of b. campestris in rats, a liquid chromatography tandem mass spectrometry (lc-ms/ms) method was developed. to avoid the interference of endogenous met in rat plasma, fetal bovine serum (fbs) was selected as surrogate matrix and validated. rat plasma was liquid-liquid extr ...201424291607
host selection behavior and the fecundity of plutella xylostella (lepidoptera: plutellidae) on multiple host plants.insect herbivores often have higher densities on host plants grown in monocultures than those in diverse environments. the underlying mechanisms are thought to be that polyphagous insects have difficulty in selecting food or oviposition sites when multiple host plants exist. however, this hypothesis needs to be extensively investigated. our field experiments revealed that the population of the diamondback moths, plutella xylostella (l.) (lepidoptera: plutellidae), significantly decreased in a mi ...201425527573
hypersensitivity to pollen of four different species of brassica: a clinico-immunologic evaluation in patients of respiratory allergy in india.rapeseed-mustard is the second most important source of edible oil in india. several species of brassica are grown in different parts of country for its oilseeds.201425379479
leaching behaviour of pendimethalin causes toxicity towards different cultivars of brassica juncea and brassica campestris in sandy loam experiment was conducted at the farm of zonal adaptive research station, uttar banga krishi viswavidhyalaya, pundibari, cooch behar, west bengal to evaluate the effect of pendimethalin on the yield, weed density and phytotoxicity in different varieties of rai (brassica juncea) and yellow sarson (b. campestris var. yellow sarson) under higher soil moisture regime in terai region of west bengal. pre-emergence application of pendimethalin at higher dose i.e. 1.0 kg/ha recorded higher plant morta ...200921217862
screening brassica species for glucosinolate content.glucosinolates (gsls), a group of compounds found in brassica plants, are toxic to some soil-borne plant pathogens because of the toxicity of their hydrolysis products, isothiocyanates. other phytochemicals found in brassica plants, such as phenols and ascorbic acid, may compliment the activity of gsls. a survey of brassica accessions from the national germplasm repository was conducted to identify potential cover crops that could be soil-incorporated for use as biofumigants. ten brassica access ...200919280485
free and bound phenolic compounds in leaves of pak choi (brassica campestris l. ssp. chinensis var. communis) and chinese leaf mustard (brassica juncea coss).eleven pak choi cultivars and two leaf mustard cultivars grown under field conditions in china were investigated for the free polyphenol content in their outer and inner leaves, as well as in their leaf blades and leaf stalks. in most cases, there were no significant differences between the hydroxycinnamic acid derivative and flavonoid derivative contents in the outer and inner leaves for the 13 cultivars. however, the contents of blades and stalks differed: hydroxycinnamic acids and flavonoids ...200826047268
phytoextraction of zinc, copper, nickel and lead from a contaminated soil by different species of a pot culture experiment, five different species of brassica (brassica juncea, brassica campestris, brassica carinata, brassica napus, and brassica nigra) were grown for screening possible accumulators of heavy metals, viz. zn, cu, ni, and pb. the plants were grown to maturity in a soil irrigated with sewage effluents for more than two decades in west delhi, india. the soil analysis showed enhanced accumulation of zn, cu, ni, and pb in this sewage-irrigated soil. among all species, b. carinat ...201318709932
composition of the phyllospheric microbial populations on vegetable plants with different glucosinolate and carotenoid compositions.the plant phyllosphere is intensely colonized by a complex and highly diverse microbial population and shows pronounced plant-species-specific differences. the mechanisms and influencing factors determining whether and in which density microorganisms colonize plant phyllosphere tissues are not yet fully understood. one of the key influencing factors is thought to be phytochemical concentration and composition. therefore, correlations between various concentrations of individual glucosinolates an ...200818183453
impact of fermentation on phenolic compounds in leaves of pak choi (brassica campestris l. ssp. chinensis var. communis) and chinese leaf mustard (brassica juncea coss).four different cultivars of chinese brassica vegetables (two pak choi cultivars and two chinese leaf mustard cultivars) were fermented according to a traditional chinese method called pickling. the plant material was investigated before and after the fermentation procedure to determine the qualitative and quantitative changes in its polyphenols. a detailed description of the identified phenolic compounds of leaf mustard by hplc-esi-ms(n) is presented here for the first time, including hydroxycin ...200818078315
the influence of plant architecture on the foraging efficiencies of a suite of ladybird beetles feeding on manipulating plant variety and predator species, we investigated the interactions of plant and predator traits in determining predation effectiveness. the predators were all coccinellid adults (hippodamia convergens, hippodamia variegata, coccinella apunctata, and coccinella septempunctata) and the prey were cabbage aphids (brevicoryne brassicae). foraging behavior of the four predators was observed on four crucifers that differed widely in their structures and surface textures (brassica oler ...199228312606
molecular cloning and expression analysis of a cu/zn sod gene (bccsd1) from brassica campestris ssp. chinensis.superoxide dismutases (sods) are a family of metalloproteins extensively exists in eukaryote, which plays an essential role in stress-tolerance of higher plants. a full-length cdna encoding cu/zn sod (bccsd1) was isolated from young seedlings of non-heading chinese cabbage (brassica campestris ssp. chinensis) by rapid amplification of cdna ends (race). bioinformatics analysis revealed that bccsd1 belonged to the plant sod super family and had the closest relationship with sod from brassica napus ...201525976826
accumulation and translocation of 198hg in four crop species.the uptake and transport of mercury (hg) through vegetation play an important role in the biogeochemical cycling of hg. however, quantitative information regarding hg translocation in plants is poorly understood. in the present study, hg uptake, accumulation, and translocation in 4 crops-rice (oryza.sativa l.), wheat (triticum l.), corn (zea mays l.), and oilseed rape (brassica campestris l.)-grown in hoagland solution were investigated using a stable isotope ((198)hg) tracing technique. the dis ...201424173818
characterisation of cell wall polysaccharides from rapeseed (brassica napus) enable structural characteristics of individual cell wall polysaccharides from rapeseed (brassica napus) meal (rsm) to be studied, polysaccharide fractions were sequentially extracted. fractions were analysed for their carbohydrate (linkage) composition and polysaccharide structures were also studied by enzymatic fingerprinting. the rsm fractions analysed contained pectic polysaccharides: homogalacturonan in which 60% of the galacturonic acid residues are methyl-esterified, arabinan branched ...201324053853
swede midge (diptera: cecidomyiidae), ten years of invasion of crucifer crops in north america.the swede midge, contarinia nasturtii kieffer (diptera: cecidomyiidae), a common insect pest in europe, is a newly invasive pest in north america that constitutes a major threat to cruciferous vegetable and field crops. since its first identification in ontario, canada, in 2000, it has rapidly spread to 65 counties in the provinces of ontario and quebec and has recently been found in canola (one of two cultivars of rapeseed, brassica napus l. and brassica campestris l.) in the central prairie re ...201121735885
using matrix solid-phase microextraction (matrix-spme) to estimate bioavailability of ddts in soil to both earthworm and vegetables.this study was conducted to find an appropriate approach for the assessment of bioavailability of ddts in soil to both earthworm and vegetables. four chemical approaches--soxhlet extraction with n-hexane, n-butanol agitation extraction, water agitation extraction, and matrix solid-phase microextraction (matrix-spme)--were used to assess the relationships between the extractability of 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (p,p'-dde), 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) e ...201019418090
[feeding preference of plutella xylostella (lepidoptera: plutellidae) larva to its hosts].the feeding experiment with different sorts of brassica vegetable crops showed that plutella xylostella larva preferred the seedlings of chinese cabbage (brassica campestris l.), radish (raphanus sativus l.) and caixin (brassica campestris l.) to those of rape (brassica napus l.) and cabbage (brassica olereacea l.), with a selection proportion of 93.33% and 6.67% between chinese cabbage and rape, and of 16.67% and 83.33% between cabbage and caixin. on the whole, the feeding preference of p. xylo ...200616964942
chemical changes induced by methyl jasmonate in oilseed rape grown in the laboratory and in the field.the effect of methyl jasmonate (mj) spraying on the chemistry of brassica plants was investigated. glucosinolates (gls) in the leaves, stems, and roots of laboratory-grown oilseed rape (brassica rapa subsp. oleifera cv. tuli and valo) 3 and 7 days after mj treatment were analyzed. volatile organic compounds (vocs) from whole oilseed rape plants were collected 3 days after mj treatment. gls were also analyzed from field-grown oilseed rape (cv. valo) treated with mj. the production of indolyl gls ...200415675811
elevated atmospheric co(2) affects the chemical quality of brassica plants and the growth rate of the specialist, plutella xylostella, but not the generalist, spodoptera littoralis.cabbage, brassica oleracea subsp. capitata (cv. lennox and rinda), and oilseed rape, brassica rapa subsp. oleifera (cv. valo and tuli), plants were grown under ambient co(2) (360 ppm) or elevated co(2) (720 ppm) at 23/18 degrees c and under a photoperiod of 22/2 h light (250 micromol m(-)(2) s(-)(1))/dark regime for up to 5 weeks. afterward, the performance of the crucifer specialist plutella xylostella (lepidoptera: plutellidae) and the generalist spodoptera littoralis (lepidoptera: noctuidae) ...200415212467
simple physical treatment as an effective tool to improve the functional properties of rapeseed (brassica campestris var. toria) and sesame seed (sesamum indicum) meals.dry and 24-h imbibed rapeseeds and sesame seeds were defatted with hexane, and freeze-dried powder was analysed for the functional properties of the meals. the water absorption capacity of the imbibed rapeseed meal and the fat absorption capacity of both the imbibed meals were higher than dry meals. protein solubility of the rapeseed meal was improved by imbibition and both the imbibed meals exhibited maximum protein solubility at ph 12. the rapeseed meal possessed better foaming properties and ...200212590740
association of rflp markers and biomass heterosis in trigenomic hybrids of oilseed rape ( brassica napus x b. campestris).laboratory screening with dna-based markers and field measurements of biomass production were carried out on each of the 120 trigenomic hybrids, obtained by interspecific hybridization between brassica napus (aacc) and brassica campestris (a'a'). the objective of this study was to elucidate the relationship between molecular markers and biomass heterosis of the interspecific hybrid between b. napus and b. campestris, which has been explored practically in rapeseed production for many years. the ...200212582933
[production and cytogenetics of hybrids of ogura cms brassica campestris var. purpuraria x raphanus sativus x brassica napus].crosses of ogura cms brassica campestris var. purpuraria x raphanus sativus x brassica napus were made and four hybrids were produced. one plant (prn-1) was mosaic with yellow and milk white flowers and some flowers had both yellow and white petals. the others (prn-2, -3, -4) had white flowers. prn-4 had degenerated anthers, the other three had three to six anthers and could produce some pollens, but the pollens of prn-2 were unstainable by i2-ki solution. prn-2 had four normal honey glands, prn ...200212043578
simple physical treatment as an effective tool to improve the functional properties or rapeseed (brassica campestris var. toria) and sesame seed (sesamum indicum) meals.dry and 24 h imbibed rapeseeds and sesame seeds were defatted with hexane and the resulting freeze-dried powder was analysed for the functional properties of the meals. water absorption capacity (wac) of imbibed rapeseed meal and fat absorption capacity (fac) of both the imbeded meals were higher than those for dry meals. protein solubility of rapeseed meals was improved by imbibition and both the imbibed meals exhibited maximum protein solubility at ph 12. rapeseed meal possessed better foaming ...200211939112
characterization of three anther-specific genes isolated from chinese cabbage.two cdna libraries were constructed from poly(a)+ rnas isolated from each of immature flowers (less than 2.0 mm long buds) and anthers (2.0-5.0 mm long buds) of chinese cabbage (brassica campestris l. ssp. pekinensis). using dot-differential hybridization, three cdna clones, designated bif38, ban54, and ban237, have been isolated from the constructed cdna libraries and sequenced completely in both directions. northern blot analyses indicate that all three cdna clones are abundantly expressed in ...19979037172
an open reading frame for a protein involved in cytochrome c biogenesis is split into two parts in brassica mitochondria.a plant mitochondrial gene with sequence similarity to ccl1, a bacterial gene involved in cytochrome c biogenesis, occurs as a single open reading frame in wheat, oenothera, carrot and marchantia mtdnas. in brassica napus "polima" or brassica campestris mtdna, however, this open reading frame is split into two segments that are located 60 kb apart on the master-circle form of the genome. although transcripts of the split orf are edited in a manner similar to that of a functional gene, transcript ...19979000383
inheritance of oilseed rape (brassica napus) rapd markers in a backcross progeny with brassica campestris.different cultivars/transgenic lines of oilseed rape (brassica napus) were crossed (as females) with different cultivars/populations of brassica campestris. all cross combinations produced seed, with an average seed set per pollination of 9.8. backcrossing of selected interspecific hybrids (as females) to b. campestris resulted in a much lower seed set, average 0.7 seed per pollination. in the single backcross progeny where a large enough population (92 plants) was obtained for analysis, 33 b. n ...199624166276
effects of inhibitors of protein serine/threonine phosphatases on pollination in brassica.we have examined the effect of the protein phosphatase inhibitors okadaic acid and microcystin on pollen-pistil interactions in brassica. inhibitor-treated flowers or floral buds were pollinated with untreated pollen and examined for pollen tube growth by fluorescence microscopy. our results show that type 1 or type 2a serine/threonine phosphatases play a crucial role in the pollination responses of brassica. we observed two distinct effects of protein phosphatase inhibitors on pollination: (a) ...199312232009
novel mitochondrial genomes in brassica napus somatic hybrids.the mitochondrial genomes of nine male-fertile and two ogura cytoplasmic male-sterile (cms) brassica napus somatic hybrids were probed with 46 mitochondrial dna fragments. the distribution of information obtained from each fusion partner was not random. several regions, including the coxi gene and a major recombination repeat sequence, were always derived from the brassica campestris fusion partner, and some regions were always derived from the ogura mitochondrial genome. novel fragments occurre ...19921356079
atrazine-resistant cytoplasmic male-sterile-nigra broccoli obtained by protoplast fusion between cytoplasmic male-sterile brassica oleracea and atrazine-resistant brassica campestris.protoplast fusion was used to combine the cytoplasmic traits of atrazine resistance and male sterility in brassica oleracea var. italica (broccoli). leaf protoplasts from broccoli with the petaloid b. nigra type of cytoplasmic male sterility were fused with hypocotyl protoplasts from an atrazine-resistant biotype of b. campestris var. oleifera cv candle (oilseed rape). a total of 19 colonies regenerated shoots, all of which were broccolilike in phenotype, i.e., lacked trichomes. four shoots, all ...199124202359
relationship between protoplast size and critical field strength in protoplast electropulsing and application to reliable dna uptake in brassica.protoplast diameter, a physical parameter controlling the susceptibility to electropermeation, was observed to vary between different batches of brassica napus and brassica campestris protoplasts. in order to control the permeation field strength associated with the protoplast size, we investigated the mathematical relationship between permeant field strength and protoplast size by measuring electroloading of the calcein dye under conditions that did not alter protoplast survival. a roughly line ...199124221493
the isolation and sequence analysis of two seed-expressed acyl carrier protein genes from brassica napus.genomic southern blot analysis of brassica napus dna indicates that seed-expressed acyl carrier protein (acp) is encoded by a multigene family of some 35 genes/haploid genome. two genomic clones encoding b. napus acp have been isolated and sequenced. the coding sequences of the 2 respective genes were found to be perfectly homologous to 2 distinct b. napus seed-expressed cdnas and therefore represent seed-expressed forms of acp. the 2 genomic acp sequences share 94% homology within their coding ...19902102833
diploid brassica napus somatic hybrids: characterization of nuclear and organellar dna.five somatic hybrids between brassica campestris and b. oleracea were obtained. molecular, morphological and cytological information all suggest that the resynthesized b. napus plants were hybrids. all five plants were diploid (2n=38) and had mainly bivalents at meiosis. seedset was low after selfing but normal after crossing with b. napus. molecular proof of the hybrid nature of these plants was obtained by hybridization of a rdna repeat to total dna. analysis of chloroplast dna restriction pat ...198824232106
mapping the chloroplast genome of triazine resistant canola.the chloroplast of a triazine resistant weed biotype of brassica campestris (bird's rape) has been transferred by repeated back-crossing into an agriculturally important strain, 'tower', of brassica napus to form a triazine resistant cultivar of canola, 'triton', that is low in both erucic acid and thioglucosinolate. in this report, the b. campestris derived chloroplast chromosome of b. napus (cv 'triton') has been cloned into bacterial plasmids and physically mapped for eight restriction enzyme ...198624247607
stability of chloroplastic triazine resistance in rutabaga backcross generations.triazine resistance originally observed in a weed biotype of birdsrape (brassica campestris l.) has been transferred through cytoplasmic substitution into rutabaga (brassica napus ssp. rapifera [metzg.] minsk.) by conventional backcrossing. photosynthetic function and resistance to triazines were examined in six backcross generations of rutabaga as well as in the original parents. chloroplast thylakoid membranes were isolated and their sensitivity to atrazine, metribuzin, and diuron assayed by m ...198616664653
initiation of postmeiotic beta-galactosidase synthesis during microsporogenesis in oilseed rape.the synthesis of beta-galactosidase during brassica campestris pollen development results from the transcription of the haploid genome. a quantitative cytochemical method has been developed in which 5-bromo-4-chloro-3-indoxyl-beta-d-galactoside is used as substrate giving a blue-green final reaction product. we have recently detected oilseed rape plants which are heterozygous for the beta-galactosidase locus, in which 50% of the pollen grains produced are gal (having enzyme activity), while the ...198516664014
influence of canola hulls on gain in weight of larvae of the yellow mealworm, tenebrio molitor l.larvae of the yellow mealworm, tenebrio molitor l., gembloux strain, race f, were reared on diets in which the protein component was supplied by defatted ground seed, defatted ground dehulled fraction, or defatted ground hulls of brassica napus l. cv. tower or brassica campestris l. cv. candle, obtained from autoclaved seed. they were also fed casein diets to which defatted ground hulls of tower or candle seed were added. gain in weight was equally good for all diets containing candle seed fract ...19836204614
morphological, cytological, and molecular characterization of hybrids and their progenies derived from the somatic hybridization of brassica campestris and brassica order to produce interspecific somatic hybrids between brassica campestris (2n = 20, aa) and brassica oleracea (2n = 18, cc), we isolated protoplasts from cotyledons and hypocotyls of young seedlings, and fused by 40% polyethylene glycol (peg). fused cells were cultured in modified k8p liquid medium supplemented with 0.2 mg/l 2,4-dichorophenoxyacetic acid (2,4-d) +0.5 mg/l 6-benzylaminopurine (6-ba)+0.1 mg/l naphthaleneacetic acid (naa)+ 0.1 mg/l kinetin (kin), 0.3 mol/l sucrose and 0.3 mol/l ...201122393713
differential parasitism of plutella xylostella (lepidoptera: plutellidae) larvae by the parasitoid cotesia plutellae (hymenoptera: braconidae) on two host plant species.laboratory experiments were conducted to examine host selection by cotesia plutellae kurdjumov when larvae of its host, plutella xylostella (linnaeus), fed on chinese cabbage, brassica campestris l. ssp. pekinensis and those fed on common cabbage, brassica oleracea l. var. capitata were provided simultaneously, and to investigate the roles of plant and host volatiles in mediating host selection. when c. plutellae were provided with equal numbers of host larvae on plants of the two species in one ...200312593684
identification and classification of s haplotypes in raphanus sativus by pcr-rflp of the s locus glycoprotein (slg) gene and the s locus receptor kinase (srk) gene.polymorphism of the s-locus glycoprotein (slg) and s-locus receptor kinase (srk) genes in raphanus sativus was analyzed by pcr-rflp using slg- and srk-specific primers. twenty four inbred lines of r. sativus could be grouped into nine s haplotypes. dna fragments of slg alleles specifically amplified from five s haplotypes by pcr with class-i slg-specific primers showed different profiles upon polyacrylamide-gel electrophoresis after digestion with restriction endonucleases. the five r. sativus s ...200212582578
isolation and characterization of pollen coat proteins of brassica campestris that interact with s locus-related glycoprotein 1 involved in pollen-stigma adhesion.adhesion of pollen grains to the stigmatic surface is a critical step during sexual reproduction in plants. in brassica, s locus-related glycoprotein 1 (slr1), a stigma-specific protein belonging to the s gene family of proteins, has been shown to be involved in this step. however, the identity of the interacting counterpart in pollen and the molecular mechanism of this interaction have not been determined. using an optical biosensor immobilized with s gene family proteins, we detected strong sl ...200010716697
striking sequence similarity in inter- and intra-specific comparisons of class i slg alleles from brassica oleracea and brassica campestris: implications for the evolution and recognition mechanism.self-incompatibility in brassica is controlled by a single multi-allelic locus (s locus), which contains at least two highly polymorphic genes expressed in the stigma: an s glycoprotein gene (slg) and an s receptor kinase gene (srk). the putative ligand-binding domain of srk exhibits high homology to the secretory protein slg, and it is believed that slg and srk form an active receptor kinase complex with a self-pollen ligand, which leads to the rejection of self-pollen. here, we report 31 novel ...19979207151
inheritance of isozyme and rflp markers in brassica campestris and comparison with b. oleracea.using primarily cdna restriction fragment length polymorphism markers (rflps) previously located to brassica oleracea (cabbage, 2n=18) chromosomes, we initiated a comparative rflp map in an f2 population of b. campestris (turnip x mock pak-choi, 2n=20). as with b. oleracea, the genome of b. campestris showed extensive gene duplication, and the majority of detected duplicated loci were unlinked. only 6 of the 49 identified loci were represented as a single copy, and 3 of these 6 were clustered on ...199124213439
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