| natural anaplasmataceae infection in rhipicephalus bursa ticks collected from sheep in the french basque country. | rhipicephalus bursa is one of 79 species of the genus rhipicephalus in the family of ixodidae. in this study, we investigated anaplasmataceae bacteria associated with r. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as r. bursa, all females were partially engorged. we found that 50% of the ticks were positive in the initial anaplasmataceae qpcr screening. bacterial species ... | 2017 | 27666778 |
| anaplasma, ehrlichia and rickettsial pathogens in ixodid ticks infesting cattle and sheep in western oromia, ethiopia. | although ticks are widely distributed in all agro-ecological zones of ethiopia, information on tick-borne pathogens is scarce. this study was conducted to determine the presence of anaplasma spp., ehrlichia spp., and rickettsia spp. in rhipicephalus evertsi and rhipicephalus (boophilus) decoloratus collected from cattle and sheep at bako, western oromia, ethiopia, using polymerase chain reaction and sequencing. anaplasma ovis and anaplasma spp., ehrlichia ruminantium and ehrlichia spp. were dete ... | 2016 | 27411938 |
| evaluation of oxidant/antioxidant status, trace mineral levels, and erythrocyte osmotic fragility in goats naturally infected with anaplasma ovis. | anaplasma ovis, an arthropod-borne pathogen that infects erythrocytes, is the major cause of ovine and caprine anaplasmosis. this study was performed to assess in goats infected with a. ovis the osmotic fragility of erythrocytes, antioxidant status, and serum levels of microminerals. blood samples were collected from 104 mixed breed goats in ahvaz area, southwest iran and subjected to parasitologic, hematologic, oxidant/antioxidant, and micromineral assessment. anaplasma infection was detected i ... | 2016 | 27142027 |
| analysis of risk factors and prevalence of haemoplasma infection in dogs. | mycoplasma haemocanis (mhc) and 'candidatus mycoplasma haematoparvum' (cmhp) are canine haemoplasma species that can induce anaemia in immunocompromised and/or splenectomised dogs. this study aimed to determine the prevalence and phylogeny of canine haemoplasma species in dogs from nigeria and describe any risk factors for infection. canine haemoplasma species-specific and generic haemoplasma qpcr assays were used. the species-specific qpcr assays found mhc infection in 18 of 245 dogs (7.3%), an ... | 2016 | 27084481 |
| evidence of co-infection with mycobacterium bovis and tick-borne pathogens in a naturally infected sheep flock. | ticks are responsible for the transmission of pathogens of veterinary importance, including those affecting sheep. the current study was designed to investigate co-infections with tick-borne and other pathogens in a naturally infected sheep flock with poor health condition using serology and pcr. infection with anaplasma ovis was detected by serology and pcr in 56% of the animals. the presence of rickettsia spp. of the spotted fever group (sfg) was detected by pcr and sequence analysis in 31% of ... | 2016 | 26726806 |
| serological investigation for west nile virus, anaplasma ovis and leishmania infantum in greek cattle. | this study investigates the seroprevalence of greek adult cattle against west nile virus, anaplasma ovis and lehismania infantum. in total, 156 serum samples were examined, drawn from cattle between 2-4 years old. all the examined cattle originated from slaughterhouses of 4 prefectures in northern greece (thessaloniki, pella, chalkidiki, kilkis), in 2 of which (thessaloniki, pella) human cases of west nile virus had been recorded some months before. thirty out of the 156 (18.6%) samples have tes ... | 2015 | 26455373 |
| human infection with a novel tick-borne anaplasma species in china: a surveillance study. | anaplasma phagocytophilum and anaplasma ovis cause human infections. we investigated the potential for human pathogenicity of a newly discovered anaplasma species infecting goats in china. | 2015 | 25833289 |
| theileria sp. ot3 and other tick-borne pathogens in sheep and ticks in italy: molecular characterization and phylogeny. | pcr reverse line blot (rlb) hybridization and sequencing were used to determine the dynamics of infection with tick-borne pathogens in one hundred apparently healthy sheep in italy. blood samples were tested once prior to the onset of the grazing season (june 2010) and once after the end of the grazing season (august 2010). ticks collected from sheep and from the vegetation were also tested by pcr/rlb. before grazing, 56% of the sheep harbored several tick-borne pathogens: anaplasma ovis was the ... | 2015 | 25448422 |
| risks of suffering tick-borne diseases in sheep translocated to a tick infested area: a laboratory approach for the investigation of an outbreak. | this study was designed to investigate an outbreak of high mortality that occurred in naïve assaf sheep introduced into a latxa sheep flock in the basque country, a region where piroplasmosis is endemic. to identify the causes of this outbreak, a panel of different methods, including traditional pathological, biopathological and parasitological analyses combined with recently developed molecular methods, was used. these novel molecular methods included a multiplex real-time pcr assay to screen f ... | 2015 | 25257849 |
| coinfection of sheep with anaplasma, theileria and babesia species in the kurdistan region, iraq. | infections of small ruminants with anaplasma, theileria and babesia species are widely distributed in the old world and are of great economic impact. in iraq, data on disease occurrence in sheep caused by above-mentioned infectious agents are scarce. this study provides information on various haemoparasitic agents infecting sheep in the kurdistan region, iraq, using molecular diagnostic tools. altogether, 195 samples originating from three governorates in the kurdistan region, namely duhok, erbi ... | 2013 | 24589110 |
| can anaplasma ovis in small ruminants be neglected any longer? | anaplasma species are obligate intracellular rickettsial pathogens transmitted by ticks with an impact on human and animal health. anaplasma ovis infects sheep and goats in many regions of the world, and it can be diagnosed by different methods like giemsa staining, pcr or competitive elisa. in this study, a pcr based on the gene coding for major surface protein 4 (msp-4) was used to examine field samples collected from sheep in different countries. altogether, 1161 blood samples from turkey (n ... | 2013 | 24589109 |
| a survey of ixodid tick species and molecular identification of tick-borne pathogens. | this study was undertaken in two different climatic areas of turkey to determine the presence of tick-borne pathogens of medical and veterinary importance. the ticks were removed from humans, pooled according to species and developmental stages, and analyzed by pcr, reverse line blot (rlb) and sequencing. of the 2333 removed ticks from 10 species, 1238 (53.06%) were obtained from the arid cold zone, and the remaining 1095 (46.93%) were obtained from the humid zone. the removed ticks were identif ... | 2014 | 24424312 |
| looking for prognosticators in ovine anaplasmosis: discriminant analysis of clinical and haematological parameters in lambs belonging to differently susceptible breeds experimentally infected with anaplasma ovis. | a study was carried out to evaluate the response of different native sheep breeds to experimental infection with anaplasma ovis, the most prevalent sheep tick-borne pathogen in apulia (southern italy). thirty-four lambs belonging to a northern european breed (suffolk) and two southern italian breeds (comisana and altamurana) were infected. eleven clinical as well as haematological parameters were monitored at different temporal resolutions on the same subjects before and after the infection, res ... | 2013 | 24053615 |
| anaplasma infections in ticks and reservoir host from slovakia. | anaplasma phagocytophilum is a worldwide distributed bacterium with a significant medical and veterinary importance. it grows within the phagosome of infected neutrophils and is responsible for human granulocytic anaplasmosis (hga), tick-borne fever (tbf) of small ruminants and cattle, canine and equine granulocytic anaplasmosis, but infects also a great variety of wildlife species. wild ungulates and rodents are considered reservoirs of infection in natural foci. the objective of this study was ... | 2014 | 23770268 |
| survey of anaplasmataceae bacteria in sheep from senegal. | the authors studied the role of bacteria belonging to anaplasmataceae family as the causes of acute illnesses of sheep in west africa. | 2013 | 23553260 |
| analysis of pathogen co-occurrence in host-seeking adult hard ticks from serbia. | past studies in serbia have reported concurrent infections of ixodes ricinus ticks with borrelia burgdorferi sensu lato genospecies, anaplasma phagocytophilum and francisella tularensis. as a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in serbia. five tick species were identified (dermacentor marginatus, d. reticulatus, haemaphysalis punctata, h. concinna and i. ricinus) and analyzed for the presence of seven pathogen ... | 2013 | 22773070 |
| a recently identified ovine babesia in china: serology and sero-epidemiology. | babesia sp. in xinjiang, transmitted by hyalomma, is a large babesia that is infective for small ruminants, but it has almost no pathogenicity in healthy sheep. on the basis of the sequences of the 18s rrna and internal transcribed spacer (its) genes, morphological characteristics, vector tick species and pathogenicity it was identified recently as a novel babesia species. in the present study, an enzyme-linked immunosorbent assay (elisa) was developed using soluble merozoite antigens of babesia ... | 2012 | 22579523 |
| molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france. | corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ... | 2017 | 28645313 |
| molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake victoria and lake baringo, kenya. | although diverse tick-borne pathogens (tbps) are endemic to east africa, with recognized impact on human and livestock health, their diversity and specific interactions with tick and vertebrate host species remain poorly understood in the region. in particular, the role of reptiles in tbp epidemiology remains unknown, despite having been implicated with tbps of livestock among exported tortoises and lizards. understanding tbp ecologies, and the potential role of common reptiles, is critical for ... | 2017 | 28620610 |
| prevalence of tick-borne haemoparasites in small ruminants in turkey and diagnostic sensitivity of single-pcr and rlb. | tick-borne haemoparasitic diseases (tbhds), caused by theileria, babesia, anaplasma and ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. many of these diseases threaten livestock production and some also represent a concern to human public health. the primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) ... | 2017 | 28449722 |
| molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock. | anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ... | 2017 | 28678004 |
| validation of an improved anaplasma antibody competitive elisa for detection of anaplasma ovis antibody in domestic sheep. | an accurate and simple-to-perform new version of a competitive elisa (celisa) kit that became commercially available in 2015 for testing of cattle for antibody to anaplasma marginale was validated for detection of anaplasma ovis antibody in domestic sheep. true positives and negatives were identified using nested pcr (npcr) as the gold standard. negative bovine control sera supplied with the kit were used to calculate % inhibition (%i), designated bovine control elisa (bcelisa), and this was com ... | 2017 | 28480803 |
| molecular detection of pathogens in ticks infesting cattle in nampula province, mozambique. | ticks are ectoparasites that can act as vectors of a large number of pathogens in wild and domestic animals, pets, and occasionally humans. the global threat of emerging or re-emerging tick-borne diseases supports the need for research focused in the zoonotic transmission, especially in countries like mozambique where rural populations are in close contact with domestic animals. the present study aims to: (1) identify tick species infesting cattle from monapo and nacala porto, districts of nampu ... | 2017 | 28856544 |
| identification of anaplasma ovis appendage-associated protein (aaap) for development of an indirect elisa and its application. | ovine anaplasmosis is a tick-borne disease that is caused by anaplasma ovis in sheep and goats. the pathogen is widely distributed in tropical and subtropical regions of the world. at present, diagnosis of the disease mainly depends on microscopy or nucleic acid based molecular tests, although a few serological tests have been applied for the detection of a. ovis infection. | 2017 | 28754151 |