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expression profiles of the p38 mapk signaling pathway from chinese shrimp fenneropenaeus chinensis in response to viral and bacterial infections.mitogen-activated protein kinase (mapk) signaling pathway plays an important role in cellular response to inflammatory cytokines, environmental stress and pathogenic infection, however, compared with mammals and insects, the potential function of p38 mapk from chinese shrimp (fenneropenaeus chinensis) in response to viruses and bacterial infection is limited. in the study, the immune responses of four genes mkk3, mkk4, p38 and atf-2 from f. chinensis were investigated in defending against white ...201829155327
the roles of β-integrin of chinese shrimp (fenneropenaeus chinensis) in wssv infection.our previous study demonstrated that an integrin β subunit of chinese shrimp (fenneropenaeus chinensis) (fcβint) plays an important role in white spot syndrome virus (wssv) infection. in the present work, in order to further elucidate the potential role of fcβint in wssv infection, the recombinant extracellular domain of β integringene of f. chinensis (rfcβint-er) was expressed in escherichia coli bl21 (de3), and the eukaryotic expression plasmid pcdna3.1-fcβint-er (pfcβint-er) was also construc ...201728686185
the identification of micrornas involved in the response of chinese shrimp fenneropenaeus chinensis to white spot syndrome virus infection.microrna (mirna) is a class of small noncoding rna, which is involved in the post-transcriptional regulation in all metazoan eukaryotes. mirnas might play an important role in the host response to virus infection. however, mirnas in the aquatic crustacean species were not extensively investigated. to obtain a better understanding of the response of chinese shrimp fenneropenaeus chinensis to white spot syndrome virus (wssv) infection, the sequence and expression profile of mirnas in the hepatopan ...201728554838
cdna cloning and expression analysis of a phosphopyruvate hydratase gene from the chinese shrimp fenneropenaeus chinensis.in the present study a cdna encoding a phosphopyruvate hydratase (enolase) was cloned from the muscle of the chinese shrimp (fenneropenaeus chinensis) and named as fcenolase. the cdna of fcenolase encoded a protein of 434 amino acid residues with a molecular mass 47.22 kda. the residues 342-355 constituted the signature motif "lllkvnqigsvtes". a snp locus (c96t) in the orf at 96 bp was identified. the results showed that the fcenolase was a conserved gene. in the normal f. chinensis, the mrna le ...201728216323
complete genome sequence of an isolate of a novel genotype of yellow head virus from fenneropenaeus chinensis indigenous in china.genotype 8 of yellow head virus (yhv-8) was identified recently, but the complete genome sequence of this new genotype has not been reported. in this study, the complete genome of yhv-8 isolate 20120706 collected from hebei province of china in 2012 was sequenced. it was found to be 26,769 nucleotides (nt) in length, including a 20,060-nt open reading frame 1 (orf1), a 435-nt orf2, and a 4971-nt orf3. isolate 20120706 shared 79.7-83.9% nucleotide sequence identity with all seven of the complete ...201728044194
haemocyanin content of shrimp (fenneropenaeus chinensis) associated with white spot syndrome virus and vibrio harveyi infection process.haemocyanin (hc) is frequently reported to vary significantly by physiological status and environmental stress in crustaceans. in this paper, the shrimp fenneropenaeus chinensis was infected with different concentrations of white spot syndrome virus (wssv) and vibrio harveyi. then, the variation of hc and total protein content of the haemolymph (tpch) were investigated using the established double antibody sandwich enzyme linked immunosorbent assay (das-elisa) and coomassie brilliant blue method ...201626616234
sumo and sumo-conjugating enzyme e2 ubc9 are involved in white spot syndrome virus infection in fenneropenaeus chinensis.in previous work, small ubiquitin-like modifier (sumo) in hemocytes of chinese shrimp fenneropenaeus chinensis was found to be up-regulated post-white spot syndrome virus (wssv) infection using proteomic approach. however, the role of sumo in viral infection is still unclear. in the present work, full length cdnas of sumo (fcsumo) and sumo-conjugating enzyme e2 ubc9 (fcubc9) were cloned from f. chinensis using rapid amplification of cdna ends approach. the open reading frame (orf) of fcsumo enco ...201626927328
recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis.anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, w ...201627517939
a novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (yhv-8) of yellow head virus.a new genotype of yellow head virus (yhv), designated as yhv-8, was found in farmed shrimp fenneropenaeus chinensis suffering suspectedly from ems/ahpns (early mortality disease/acute hepatopancreatic necrosis disease) in china in 2012. in this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rrt-lamp) assay was developed for better detection of both genotypes of yhv-1 and yhv-8. a set of six specific primers was successfully designed targeting a conserv ...201627221155
comparative microarray profile of the hepatopancreas in the response of "huanghai no. 2" fenneropenaeus chinensis to white spot syndrome virus.white spot syndrome virus (wssv) infects all shrimp species and is the greatest detriment to shrimp culture. to better understand the mechanism of molecular responses to wssv infection in "huanghai no. 2" fenneropenaeus chinensis, a microarray technique was used. microarray gene expression profiling of 59,137 unigenes identified differentially expressed genes (degs) both in live and moribund shrimp at early, peak and late phases. in live shrimp, 1307, 1479 and 1539 degs were obtained in the earl ...201627591045
virus-derived small rnas in the penaeid shrimp fenneropenaeus chinensis during acute infection of the dna virus wssv.small interfering rnas (sirnas) and micrornas (mirnas) are two classes of small rnas (srnas) that are critical for virus-host interplay via the rna interference (rnai) pathway. one virus-derived sirna and numerous mirnas has been reported for the double-stranded dna virus white spot syndrome virus (wssv), however, the expression profiles of these different types of srnas have not been assessed. here, by sequencing the srnas and mrnas of wssv-infected chinese shrimp (fenneropenaeus chinensis), we ...201627349643
dietary supplementation of probiotic bacillus pc465 isolated from the gut of fenneropenaeus chinensis improves the health status and resistance of litopenaeus vannamei against white spot syndrome virus.this study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic bacillus pc465 on the growth, health status, and disease resistance of litopenaeus vannamei. shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) cfu g(-1)]. probiotic supplementation significantly increased the weight gain and survival of l. vannamei (p < 0.05). the effect of ...201627177431
isolation and expression analysis of an mapkk gene from fenneropenaeus chinensis in response to white spot syndrome virus infection.mitogen-activated kinase kinase (mapkk) is an important gene involved in the host-virus interaction process. to obtain a better understanding of mapkk in the interaction process between the chinese shrimp fenneropenaeus chinensis and white spot syndrome virus (wssv), we cloned the sequence of an mapkk cdna from f. chinensis (fcmapkk) and investigated the effect of fcmapkk on wssv infection. the results showed that the fcmapkk gene contained a 1227 bp open reading frame (orf), which encoded a hig ...201627164214
cloning of litopenaeus vannamei cd63 and it's role in white spot syndrome virus infection.white spot syndrome virus (wssv) is currently the most serious shrimp pathogen, which has brought huge losses to shrimp industry worldwide. cd63 of shrimp belongs to the tetraspanin superfamily, which plays an important role in signal transduction and immune process. in this paper, cd63 cdna sequence of litopenaeus vannamei was cloned using race method. the amplified sequence is 1472 bp, with its orf 744 bp, encoding 247 amino acids. bioinformatics analysis showed that the sequence of lvcd63 has ...201626964710
pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of fenneropenaeus chinensis farming pond.a novel aerobic bacterium, gram-stain-negative, motile, oxidase- and catalase-positive, non-spore-forming, non-pigmented, designated rzy34t, was isolated from the sediment of fenneropenaeus chinensis farming pond, rizhao, china. the strain was able to grow at ph 6-10 (optimum ph 7), 20-40 °c (optimum 30 °c) and in the presence of 1.0-6.0% nacl (optimum 1.0-2.0%). phylogenetic analysis based on 16s rrna gene sequences revealed that the nearest relative of strain rzy34t was pseudoalteromonas xiame ...201627149865
recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (fcalf5) from chinese shrimp fenneropenaeus chinensis.antimicrobial peptides (amps) have a great potential to be used as a substitute for antibiotics since amps don't lead to bacteria's drug resistance. anti-lipopolysaccharide factors (alfs) are one type of amps and exist in crustaceans. in the present study, we produced a recombinant protein (rfcalf5) of an alf isoform (fcalf5) from chinese shrimp fenneropenaeus chinensis through a prokaryotic expression system. the rfcalf5 exhibited varied antibacterial activities against different bacteria. besi ...201526123888
genomic organization and promoter analysis of a transcriptional repressor gene from fenneropenaeus chinensis.in this study, we cloned and sequenced genomic sequences from a fenneropenaeus chinensis transcriptional repressor gene, fctr. the fctr gene is 2,671 bp in length and has four exons and three introns. the 873 bp promoter contains several transcription factor binding sites, including a tata box and a cyclic amp-responsive element. promoter deletion analysis using a luciferase reporter gene identified regulatory elements. challenge with white spot syndrome virus increased expression from the promo ...201525266240
cloning and characterization of calreticulin and its association with salinity stress in p. trituberculatus.calreticulin (crt) is a highly conserved and multifunctional endoplasmic reticulum (er) chaperone protein and plays important roles in salinity stress response. portunus trituberculatus is a commercially important fishery species, and water salinity conditions influence its commercial farming significantly. in order to research the function of calreticulin under salinity stress, the full-length cdna sequence of calreticulin from p. trituberculatus (ptcrt) was firstly cloned and characterized. th ...201525995067
a new nodavirus is associated with covert mortality disease of shrimp.a new nodavirus, named covert mortality nodavirus (cmnv), is associated with covert mortality disease of shrimp which has caused serious loss in china since 2009. histopathological examination of shrimp suffering the disease revealed coagulative necrosis of striated muscle similar to typical histopathology features of infectious myonecrosis virus (imnv), penaeus vannamei nodavirus (pvnv) and macrobrachium rosenbergii nodavirus (mrnv). however, shrimp suffering this disease tested negative for im ...201425205685
characterization and function analysis of an anti-lipopolysaccharide factor (alf) from the chinese shrimp fenneropenaeus chinensis.anti-lipopolysaccharide factor (alf) is one of the widely-studied antimicrobial peptides (amps) with broad-spectrum antibacterial activity and antiviral property. previous studies show the existence of multiform of alfs in crustacean which are important for immunity of the animals. in the present study, we characterized one isoform of alf from the chinese shrimp fenneropenaeus chinensis (fcalf2). tissue distribution analysis revealed that fcalf2 showed the highest expression level in the lymphoi ...201424879940
functional characterization of a c-type lysozyme from indian shrimp fenneropenaeus indicus.lysozyme gene from fenneropenaeus indicus was cloned, expressed in escherichia coli and characterized. the cdna consists of 477 base pairs and encodes amino acid sequence of 159 residues. f. indicus lysozyme had high identity (98%) with fenneropenaeus merguiensis and fenneropenaeus chinensis and exhibits low to moderate identities with lysozymes of other invertebrates and vertebrates. this lysozyme is presumed to be chicken types as it possesses two catalytic and eight cysteine residues that are ...201424676722
assessment of microwave cooking on the bioaccessibility of cadmium from various food matrices using an in vitro digestion model.bioaccessibility represents the maximum amount of pollutant ingested with food that is available for intestinal absorption. the measurement of bioaccessibility can achieve a more accurate risk assessment. thus, in this study, the bioaccessibility of raw/microwave-cooked store-bought food including carrot, potato, white radish, lotus root, sweet corn, long grain rice, soybean, fleshy prawn, eastern oyster, kelp, and common carp were investigated by applying an in vitro digestion method. a validat ...201424958019
cloning and characterization of cytoplasmic dynein intermediate chain in fenneropenaeus chinensis and its essential role in white spot syndrome virus infection.to investigate the role of cytoplasmic dynein in white spot syndrome virus (wssv) infection, the full-length cdna of cytoplasmic dynein intermediate chain (fcdynci) was cloned in fenneropenaeus chinensis, which consists of 2582 bp and encodes a polypeptide of 660 amino acids. sequence analysis and multiple sequence alignment displayed that fcdynci was a member of cytoplasmic dynein 1 family. the fcdynci mrna was most highly expressed in hemocytes, which was significantly up-regulated post wssv i ...201424925758
a new anti-lipopolysaccharide factor (alf) gene with its snp polymorphisms related to wssv-resistance of litopenaeus vannamei.anti-lipopolysaccharide factors (alfs) of crustacean play an important role against bacteria or virus infection. in this study, the cdna sequence and genomic sequence of one new isoform of alf designated as nlvalf1 were reported. the open reading frame (orf) of nlvalf1 consisted of 369 bp encoding 123 amino acids and the genomic structure of nlvalf1 comprised four introns and three exons. the predicted pi of the deduced protein was 8.82 and the molecular weight (mw) was 13.72 kda. the deduced am ...201424769128
full-length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the indian white prawn, fenneropenaeus indicus (h. milne edwards).elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture-reared shrimps is pivotal for the prevention and/or control of disease outbreaks. in this study, we examined the phylogenetic relatedness and compared the possible immune-related functional roles of both translationally controlled tumour protein (tctp) and ferritin genes with previous studies. both tctp and ferritin genes were substantially upregulated in the indian white prawn, fenneropenaeus ...201424329985
proteomic analysis of differentially expressed proteins in fenneropenaeus chinensis hemocytes upon white spot syndrome virus infection.to elucidate molecular responses of shrimp hemocytes to white spot syndrome virus (wssv) infection, two-dimensional gel electrophoresis was applied to investigate differentially expressed proteins in hemocytes of chinese shrimp (fenneropenaeus chinensis) at 24 h post infection (hpi). approximately 580 protein spots were detected in hemocytes of healthy and wssv-infected shrimps. quantitative intensity analysis revealed 26 protein spots were significantly up-regulated, and 19 spots were significa ...201424587154
cloning of hsp21 gene and its expression in chinese shrimp fenneropenaeus chinensis in response to wssv challenge.in the present study, a dna sequence encoding a small heat shock protein gene (fchsp21) in the chinese shrimp, fenneropenaeus chinensis, was cloned, and its expression was analyzed after white spot syndrome virus (wssv) infection. the fchsp21 gene contained an open reading frame (orf) of 555 bp in length, encoding a 184 amino acid protein with a theoretical size of about 21 kda and a predicted isoelectric point of 5.38. the mrna of the hsp21 had a long poly(a) tail (748 bp) with six polyadenylat ...201424442516
molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in fenneropenaeus chinensis and its antioxidant activity.peroxiredoxins (prx) are a family of antioxidant proteins and perform important functions in intracellular signal transduction. here, we report a prx gene from chinese shrimp fenneropenaeus chinensis. the full-length cdna of fcprx gene contained an open reading frame of 735 bp encoding a polypeptide of 275 amino acids. the molecular mass of the deduced amino acid of fcprx is 27445.43 da with an estimated pi of 5.71. sequence comparison showed that the fcprx shares high identities with prx ivs an ...201424418456
molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from oriental river prawn, macrobrachium nipponense.the lipopolysaccharide and β-1,3-glucan binding protein (lgbp), one of the pattern recognition proteins, plays an important role in the innate immune response of invertebrates. a 1,506 bp full-length cdna of a lgbp gene was cloned and characterized from the oriental river prawn macrobrachium nipponense (named as mnlgbp). analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,119 bp, encoding a protein of 372 amino acids including a 21-aa signal peptide. t ...201424584659
comparison of protein expression profiles of the hepatopancreas in fenneropenaeus chinensis challenged with heat-inactivated vibrio anguillarum and white spot syndrome virus.fenneropenaeus chinensis (chinese shrimp) culture industry, like other penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. to better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. firstly, the soluble hepatopancreas protein samples in adult chinese shrimp among control, heat-inactivated vibrio-challenged and white spot syndrome virus-infected groups were separated ...201424057166
characterization of an immune deficiency homolog (imd) in shrimp (fenneropenaeus chinensis) and crayfish (procambarus clarkii).the immune deficiency (imd) signal pathway mediates immunity against gram-negative bacteria in drosophila. recent studies show that the imd pathway also involves in antiviral innate immune responses. the functions of the pathway in crustacean immunity are largely unknown. in this paper, two imds (fcimd and pcimd), one of the key elements of the imd pathway, were identified from chinese white shrimp fenneropenaeus chinensis and red swamp crayfish procambarus clarkii. both proteins have a death do ...201323850721
shrimp myd88 responsive to bacteria and white spot syndrome virus.the myeloid differentiation factor 88 (myd88) is an important adapter protein which links members of the toll-like receptor (tlr) to the downstream components to activate related signaling pathways. in the present study, a myd88 homolog (fcmyd88) was cloned from penaeid shrimp fenneropenaeus chinensis. the orf of fcmyd88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (dd) and a typical tlr and interleukin-1 receptor (il-1r)-related (tir) domain. homo ...201323238389
the vp37-binding protein f1atp synthase β subunit involved in wssv infection in shrimp litopenaeus vannamei.to investigate the interaction between white spot syndrome virus (wssv)-vp37 and gill membrane proteins (gmps) of pacific white shrimp (litopenaeus vannamei), the vp37 protein was expressed and purified, and a distinct 53 kda vp37-binding protein band was identified in gmps by virus overlay protein binding assay and gst pull-down assay. by electroelution, the vp37 binding protein was purified and identified as f(1)atp synthase β (f(1)atpase β) subunit by mass spectrometry. the purified f(1)atpas ...201323108256
role of chymotrypsin-like serine proteinase in white spot syndrome virus infection in fenneropenaeus chinensis.white spot syndrome virus (wssv) caused a great economic loss in shrimp aquaculture. although great efforts have been undertaken to characterize the virus disease during the last two decades, there are still lack of effective methods to prevent or cure it. in this study, we investigated the transcriptional expression profiles of 18 key immune-related genes in the chinese shrimp fenneropenaeus chinensis which was severely infected by wssv. we found that the expression levels of 6 genes including ...201323142726
an evolutionarily ancient no synthase (nos) in shrimp.nitric oxide (no) is a well known essential molecule that is involved in multiple functions such as neuron transduction, cardiac disease, immune responses, etc.; nitric oxide synthase (nos) is a critical enzyme that catalyzes the synthesis of it. a very few crustacean nos molecules were biochemically characterized so far. in the present study, we cloned and characterized a nos cdna from haemocytes of tiger shrimp (penaeus monodon) (pmnos). the full-length of pmnos cdna contained 3997 bp, includi ...201323994281
detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time pcr assay.as one of the major pathogens, hepatopancreatic parvovirus (hpv) can cause severe diseases in penaeid shrimp. we developed a taqman-based real-time pcr assay for the hpv detection in china. a pair of primers (hpvf and hpvr) and a taqman probe were designed according to the hpv genomic sequence of chinese isolate (genbank: gu371276). our data showed that the primers and taqman probe were specific for hpv, and they exhibited no cross-reaction with infectious hypodermal and hematopoietic necrosis v ...201324090693
genomic organization and promoter characterization of fcctl, a c-type lectin-like protein in fenneropenaeus chinensis.diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. c-type lectins may play an important role in shrimp immunity. the gene encoding fcctl, a c-type lectin-like protein, was isolated from fenneropenaeus chinensis and characterized. this gene was composed of five exons spanning 2470 base pairs (bp). intron 1 contained a possible promoter and many potential transcription factor binding sites, such as those for tata binding protein, creb, srf, ttk and three sites f ...201323337594
identification and molecular characterization of a c-type lectin-like protein from chinese shrimp (fenneropenaeus chinensis).a c-type lectin-like protein was cloned and characterized from the chinese shrimp fenneropenaeus chinensis, and named as fcctl. the results indicated that the full length cdna of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved cys and one key motif epgd. the theoretical molecular weight and pi of mature protein was 25.3 kda and 5.4. sequence comparison of the deduced amino acid sequence of fcctl showed varied ident ...201323192616
transcriptome analysis on chinese shrimp fenneropenaeus chinensis during wssv acute infection.previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (wssv) infection in crustacean. however, little information is available in relation to underlying mechanisms of host responses during the wssv acute infection stage in naturally infected shrimp. in this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. a tot ...201323527000
identification, cloning and characterization of an extracellular signal-regulated kinase (erk) from chinese shrimp, fenneropenaeus chinensis.extracellular signal-regulated kinase (erk) is a serine/threonine-specific protein kinase, which participates in signaling transduction pathways that control intracellular events, including resumption of meiosis, embryogenesis, cell differentiation, cell proliferation, cell death and response to radiation. some virus species evolved the ability to hijack the host cell erk signaling transduction pathway for viral replications and gene expressions. to obtain a better understanding of erk, we clone ...201324084041
molecular cloning and characterization of a cathepsin b gene from the chinese shrimp fenneropenaeus chinensis.cathepsin b is a unique member of the cathepsin superfamily, which acts as both an endopeptidase and peptidyl-dipeptidase. to obtain a better understanding of this enzyme, we cloned a cdna encoding cathepsin b from the muscle of fenneropenaeus chinensis (fccb). fccb contained a 996-bp open reading frame (orf) encoding a protein of 331 amino acid residues with a putative signal peptide and a propeptide_c1 at the n-terminal, a glutamine oxyanion hole and active site cysteine, histidine and asparag ...201324041842
expression kinetics of β-integrin in chinese shrimp (fenneropenaeus chinensis) hemocytes following infection with white spot syndrome virus.our previous study has demonstrated that an integrin β subunit of chinese shrimp (fenneropenaeus chinensis) (fcβint) involved in wssv infection. in order to further elucidate the potential role of the fcβint in the wssv infection, expression response of fcβint to wssv infection in shrimp hemocytes was investigated after intra-muscular injection with the virus. following time-course hemocytes sampling, the expression variation of fcβint in hemocytes was examined by flow cytometric immunofluoresce ...201323711468
identification and characterization of a serine protease inhibitor esserpin from the chinese mitten crab eriocheir sinensis.serine protease inhibitors (serpins) represent an expanding superfamily of endogenous inhibitors that regulate proteolytic events and involve in a variety of physiological processes. a serine protease inhibitor, namely esserpin, was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) analysis. the full-length cdna of esserpin was of 2367 bp, including an open reading frame (orf) of 1371 bp encoding a polypeptide of 456 amino acids with estimated molecular ...201323567854
identification and characterization of the sex-determiner transformer-2 homologue in chinese shrimp, fenneropenaeus chinensis.the transformer-2 gene, encoding a protein (tra-2) which directs sex-specifically alternative splicing of doublesex (dsx) pre-mrna in combination with the transformer (tra) protein, has been proved to play important roles on sex differentiation and sex development in drosophila melanogaster. in the present study, a tra-2 homologue (fctra-2) was cloned and characterized in the chinese shrimp, fenneropenaeus chinensis. a fctra-2 genomic dna sequence with a length of 8,871 bp was obtained and verif ...201222846336
molecular cloning, expression analysis and characterization of peroxiredoxin during wssv infection in shrimp fenneropenaeus indicus.innate immunity is the first line of defense in shrimps against invading pathogenic microorganisms. peroxiredoxins (prx) are the family of antioxidant proteins that play a crucial role in reduction of oxidative stress in host during viral infection. peroxiredoxin from fenneropenaeus indicus was identified, cloned and expressed in prokaryotic expression system. the fi-prx protein consists of two conserved cys residues and belongs to typical 2-cys prx family. the phylogenetic analysis revealed a c ...201221964313
molecular cloning, sequence analysis and expression of fein-penaeidin from the haemocytes of indian white shrimp fenneropenaeus indicus.penaeidins are members of a special family of antimicrobial peptide existing in penaeid shrimp and play an important role in the immunological defense of shrimp. here, we report a penaeidin sequence cloned from the indian white shrimp fenneropenaus indicus (fein-penaeidin). the fein-penaeidin open reading frame encodes a 77 amino acid peptide including a 19 amino acid signal peptide. the deduced amino acid sequences of fein-penaeidin include a proline rich n-terminal domain and a carboxyl-domain ...201224371565
blocking the large extracellular loop (lel) domain of fctetraspanin-3 could inhibit the infection of white spot syndrome virus (wssv) in chinese shrimp, fenneropenaeus chinensis.tetraspanins belong to the transmembrane 4 superfamily (tm(4)sf), which span the cell membrane 4 times and act as bridges or connectors. increasing evidences have shown that tetraspanins play important role in virus infection. the large extracellular loop (lel) of a tetraspanin is considered as a possible target of some virus. tetraspanins are widely found in invertebrates, but the functional roles of most invertebrate tetraspanins have remained unknown. recently, a tetraspanin, called fctetrasp ...201222406449
involvement of fenneropenaeus chinensis cathepsin c in antiviral immunity.cathepsin c (cath c) is a lysosomal cysteine protease that belongs to the papain superfamily. cath c is capable of activating many chymotrypsin-like serine proteases and is reported to be a central coordinator for the activation of many serine proteinases in immune and inflammatory cells. in this study, cath c cdna was cloned from fenneropenaeus chinensis (fc). the complete cdna of fc-cath c in chinese white shrimp was found to be 1445-base pairs (bp) long. it contained an open reading frame (or ...201222885030
A novel pathogen-binding gC1qR homolog, FcgC1qR, in the Chinese white shrimp, Fenneropenaeus chinensis.In vertebrates, the globular "head" of complement component C1q receptor (gC1qR) is a versatile, multiligand binding protein. However, research on its function in invertebrates is limited. In the present study, a full-length cDNA sequence of a novel gC1qR homolog, FcgC1qR, from the Chinese white shrimp Fenneropenaeus chinensis was cloned. Semi-quantitative polymerase chain reaction (PCR) detected FcgC1qR in all examined tissues, with the highest level detected in the intestine. Western blot assa ...201221893092
an iκb homologue (fccactus) in chinese shrimp fenneropenaeus chinensis.this study firstly reports the characterization of a functional iκb homologue, fccactus in chinese shrimp fenneropenaeus chinensis. the full length cdna of fccactus consists of a 1359 bp open reading frame (orf) encoding a 453 amino acid protein with a predicted molecular weight (mw) of 48.46 kda and theoretical pi of 5.23. phylogenetic analysis and multiple alignments revealed that the deduced amino acid sequence of fccactus cdna had high similarities to cactus or iκb reported in seven other ar ...201223276882
trbp and eif6 homologue in marsupenaeus japonicus play crucial roles in antiviral response.plants and invertebrates can suppress viral infection through rna silencing, mediated by rna-induced silencing complex (risc). trans-activation response rna-binding protein (trbp), consisting of three double-stranded rna-binding domains, is a component of the risc. in our previous paper, a trbp homologue in fenneropenaeus chinensis (fc-trbp) was reported to directly bind to eukaryotic initiation factor 6 (fc-eif6). in this study, we further characterized the function of trbp and the involvement ...201222279564
molecular cloning and expression analysis of signal transducer and activator of transcription (stat) from the chinese white shrimp fenneropenaeus chinensis.innate immunity is the first line of defense by a host against invading pathogens. several signaling pathways participate in the immune response, one of which is the janus kinase (jak)/signal transducer and activator of transcription (stat) pathway. various evidences have been provided to suggest that the jak/stat pathway is involved in both antibacterial and antiviral immunities. in this study, the full-length cdna and gene sequence of stat (designated as fcstat) was cloned from the chinese whi ...201121246285
cloning and characterization of rap gtpase from the chinese white shrimp fenneropenaeus chinensis.ras-related protein rap gtpase has been implicated in cell adhesion, cell proliferation, and cell junction formation. the first shrimp rap cdna (fcrap) was recently identified from the chinese white shrimp fenneropenaeus chinensis. the full length of fcrap is 1013bp, with a 561bp open reading frame that encodes a 186 amino acid protein. fcrap has a calculated molecular mass of 20.90kda and pi of 6.37. phylogenetic analysis shows that fcrap and other rap proteins are clustered into one group. res ...201121801745
a homolog of the cell apoptosis susceptibility gene involved in ovary development of chinese shrimp fenneropenaeus chinensis.the cell apoptosis susceptibility (cas) gene is a homolog of the yeast chromosome segregation (cse1) gene, which functions in cell proliferation and apoptosis. in the present study, a homolog of cas was cloned from chinese shrimp fenneropenaeus chinensis (fccas). the full-length fccas cdna is 3534 bp and contains an open reading frame encoding 968 amino acids. the predicted tertiary fccas structure is highly similar to that of cse1 from the yeast saccharomyces cerevisiae. rt-pcr analysis showed ...201121900679
molecular cloning and characterization of a receptor for activated protein kinase c1 (rack1) from chinese white shrimp; fenneropenaeus chinensis.receptor for activated c kinase 1 (rack1), which has seven tandem wd40 domains, is a scaffolding protein. rack1 plays different roles by binding to different partner proteins. it is involved in hormone signaling and development, and now some evidence indicates it may have a role in innate immunity. in this paper, rack1 cdna from chinese white shrimp (fcrack1) was identified. the full length of the fcrack1 gene is 1037 bp, including a 30 bp 5'utr, a 957 bp orf encoding a 318 amino acid protein, a ...201121238484
involvement of peroxinectin in the defence of red swamp crayfish procambarus clarkii against pathogenic aeromonas hydrophila.cell adhesion factors are important immune components for invertebrate to immobilize, phagocytose or encapsulate invasive microorganisms and foreign particles. in this study, a new cell adhesion factor, peroxinectin (refered as pcpxin) was isolated from hemocytes of red swamp crayfish (procambarus clarkii). the full-length cdna of pcpxin was 3014 bp encoding a protein of 819 amino acid residues with a predicted molecular weight of 89.0 kda and a calculational isoelectric point of 6.93. the putat ...201121539922
enzyme e2 from chinese white shrimp inhibits replication of white spot syndrome virus and ubiquitinates its ring domain proteins.recent studies have shown that the ubiquitin (ub) proteasome pathway (upp) is closely related to immune defense. we have identified a ubiquitin-conjugating enzyme, e2, from the chinese white shrimp, fenneropenaeus chinensis (fcubc). injection of recombinant fcubc protein (rfcubc) reduced the mortality of shrimp infected with white spot syndrome virus (wssv) and inhibited replication of wssv. rfcubc, but not a mutant fcubc (mfcubc), bound to wssv ring domains (wrds) from four potential e3 ligase ...201121680526
A TaqMan Real-Time PCR Assay for Quantifying Type III Hepatopancreatic Parvovirus Infections in Wild Broodstocks and Hatchery-Reared Postlarvae of Fenneropenaeus chinensis in Korea.A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatcheryreared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders w ...201122127120
complete nucleotide sequence analysis of a korean strain of hepatopancreatic parvovirus (hpv) from fenneropenaeus chinensis.hepatopancreatic parvovirus (hpv) of shrimp is distributed worldwide and the entire genome of thailand and indian strains (pmdnv) and one australian strain (pmergdnv) have now been reported. the complete nucleotide sequence of a hpv strain isolated from the fleshy prawn fenneropenaeus chinensis in korea (fcdnv) was determined and compared to previously reported sequences. the entire genome of fcdnv contains 6,336 nucleotides, with 40% g+c content, which is the biggest of the known hpv strains. t ...201121948006
multiple forms of alpha-2 macroglobulin in shrimp fenneropenaeus chinesis and their transcriptional response to wssv or vibrio pathogen infection.alpha-2 macroglobulin (a2m) is a non-specific protease inhibitor involved in host defense. by full length cloning and sequencing we identified three distinct cdnas for a2m in chinese shrimp fenneropenaeus chinesis, designated fca2m-1, fca2m-2 and fca2m-3, respectively. expression profiles in normal tissues as well as tissues after challenge by white spot syndrome virus (wssv) and vibrio pathogen were conducted for fca2m-1 and fca2m-2. the fca2m-1 and fca2m-2 cdnas encode proteins with 1501 or 15 ...201020105438
molecular cloning and expression pattern analysis of two novel disulfide isomerases in shrimp.protein disulfide isomerase (pdi) catalyzes formation and isomerization of disulfide bridges and has chaperone activity. currently, increasing evidence suggests the significance of pdi in immune and stress responses. to clarify the role of pdis in the innate immunity of shrimp, two pdi genes were isolated and identified from fenneropenaeus chinensis (fleshy prawn). fcpdi1 is 1878bp in length and encodes a protein of 383 amino acids. it has 18-amino acid signal peptide, 3 thioredoxin domains with ...201021130898
the possible role of penaeidin5 from the black tiger shrimp, penaeus monodon, in protection against viral infection.penaeidin class 5 (pen5) has so far only been reported in the chinese shrimp, fenneropenaeus chinensis, and the black tiger shrimp, penaeus monodon. the pen5 homolog from f. chinensis (fenchipen5) exhibits antimicrobial activities against both gram-positive and gram-negative bacteria as well as fungi. here, we characterized the pen5 gene from p. monodon (penmonpen5) and evaluated its potential involvement in antiviral immunity. the deduced open reading frame of penmonpen5 encodes for a predicted ...201021199664
vibrio atypicus sp. nov., isolated from the digestive tract of the chinese prawn (penaeus chinensis o'sbeck).a culture (designated strain hhs02(t)) was isolated from chinese prawn (penaeus chinensis, o'sbeck) and determined to be a member of the genus vibrio. strain hhs02(t) comprised slightly curved, rod-shaped, non-endospore-forming, gram-negative, catalase-negative, oxidase-positive, o/129-sensitive and facultatively anaerobic cells that were motile by means of a single polar flagellum. growth of strain hhs02(t) occurred in 0.5-7 % (w/v) nacl [optimally in 1-3 % (w/v) nacl] and between ph 7.0 and 10 ...201019965995
predicted sub-populations in a marine shrimp proteome as revealed by combined est and cdna data from multiple penaeus species.abstract:201021067619
a new c-type lectin (fclec5) from the chinese white shrimp fenneropenaeus chinensis.c-type lectins are one family of pattern recognition receptors (prrs) that play important roles in innate immunity. in this work, cdna and genomic sequences for a new c-type lectin (fclec5) were obtained from the chinese white shrimp fenneropenaeus chinensis. fclec5 cdna contains an open reading frame of 1,008 bp and its genomic sequence is 1,137 bp with 4 exons and 3 introns. the predicted fclec5 protein contains a signal peptide and two carbohydrate recognition domains (crds). the n-terminal c ...201020349323
proteomic analysis of differentially expressed proteins in lymphoid organ of fenneropenaeus chinensis response to vibrio anguillarum stimulation.to gain an insight into the function of shrimp lymphoid organ at protein level, we analyzed the proteome of lymphoid organ in healthy chinese shrimp fenneropenaeus chinensis (f. chinensis) through two-dimensional gel electrophoresis (2-de) based proteomic approach. a total of 95 spots representing 75 protein entries were identified by liquid chromatography tandem mass spectrometry (lc-ms/ms) with both online and in-house database. according to gene ontology (go) annotation of biological process, ...201020394824
identification of a novel inducible cytosolic hsp70 gene in chinese shrimp fenneropenaeus chinensis and comparison of its expression with the cognate hsc70 under different stresses.the heat shock protein 70 (hsp70) family is widely expressed in eukaryotic cells as the major chaperone protein. in this study, the full-length complementary dna (cdna) of a novel inducible cytosolic hsp70 family member (fchsp70) was cloned from fenneropenaeus chinensis. fchsp70 full-length cdna consists of 2,511 bp with a 1,890-bp open reading frame encoding 629 amino acids. three hsp70 protein family signatures, idlgttys, iidlgggtfdvsil, and ivlvggstripkvqk, were found in the predicted fchsp70 ...201019496024
immune responses of fenneropenaeus chinensis against white spot syndrome virus after oral delivery of vp28 using bacillus subtilis as vehicles.the protective efficacy of oral administration of vp28 using bacillus subtilis as vehicles (rvp28-bs) in shrimp, fenneropenaeus chinensis, upon challenge with white spot syndrome virus (wssv) was investigated. the calculated relative percent survival (rps) value of rvp28-bs fed shrimp was 83.3% when challenged on the 14th day post-administration, which is significantly higher (p < 0.001) than that of the group administered recombinant escherichia coli over-expressing rvp28 (rvp28-e21). after imm ...201019800009
three tetraspanins from chinese shrimp, fenneropenaeus chinensis, may play important roles in wssv infection.three members of the tetraspanin/tm(4)sf superfamily were cloned from chinese shrimp, fenneropenaeus chinensis. the deduced amino acid sequences of the three proteins have typical motifs of the tetraspanin/tm(4)sf superfamily. phylogenetic analysis of the proteins, together with the known tetraspanins of invertebrates and vertebrates, revealed that they belong to different tetraspanin subfamilies: cd9, cd63 and tetraspanin-3. the three cloned genes of cd9, cd63 and tetraspanin-3 showed apparentl ...201019943839
a dorsal homolog (fcdorsal) in the chinese shrimp fenneropenaeus chinensis is responsive to both bacteria and wssv challenge.rel/nfkappab is a family of transcription factors. in the present study, a rel/nfkappab family member, dorsal homolog (fcdorsal) was cloned from the chinese shrimp fenneropenaeus chinensis. the full length cdna of fcdorsal consists of 1627bp, revealed a 1071bp open reading frame encoding 357 aa. the predicted molecular weight (mw) of the deduced amino acid sequence of fcdorsal was 39.78kda, and its theoretical pi was 8.85. amino acid sequence analysis showed that fcdorsal contains a rel homolog ...201020363249
comparative proteomic profiles of the hepatopancreas in fenneropenaeus chinensis response to white spot syndrome virus.white spot syndrome virus (wssv) infects all shrimp species and is the greatest detriment to shrimp culture. to better understand the mechanism of molecular responses to wssv infection in chinese white shrimp fenneropenaeus chinensis, two dimensional electrophoresis (2-de) was used. differentially expressed proteins in the hepatopancreas of control and wssv-injected chinese white shrimp between (6, 12 and 24 h post-injection) were screened. quantitative intensity analysis and mass spectrometry r ...201020580833
molecular characterization and expression analysis of chitinase (fcchi-3) from chinese shrimp, fenneropenaeus chinensis.complementary dna (cdna) and genomic dna, including flanking regions of the chitinase gene (fcchi-3) of fenneropenaeus chinensis, were cloned and sequenced. fcchi-3 was found to have 92.0 and 91.4% identity at the cdna level to that of litopenaeus vannamei and marsupenaeus japonicus, respectively. the predicted amino acid sequence had an overall similarity with a comparable region of l. vannamei (96.8%) and m. japonicus (93.4%). based on the cdna sequence, the genomic structure of the gene was c ...201019642017
a thioredoxin response to the wssv challenge on the chinese white shrimp, fenneropenaeus chinensis.thioredoxin (trx) is involved in cell redox homeostasis. in addition, it is responsible for maintaining proteins in their reduced state. in our study, a fenneropenaeus chinensis thioredoxin (fctrx) gene was identified from the chinese white shrimp. the full length of fctrx was 777 bp, including a 60 bp 5' untranslated region (utr), a 318 bp open reading frame (orf) encoding a 105 amino acids protein, and a 399 bp 3' utr. fctrx contained a trx domain with a conserved motif of cys-gly-pro-cys (cgp ...201019747568
two cysteine proteinases respond to bacterial and wssv challenge in chinese white shrimp fenneropenaeus chinensis.the cdnas encoding cathl and legumain from chinese white shrimp fenneropenaeus chinensis (fccathl, fclegu) were obtained. both fccathl and fclegu mrna were expressed mainly in the hepatopancreas of unchallenged shrimp. time-course analysis of fccathl showed that fccathl was upregulated in the hepatopancreas of shrimp challenged with white spot syndrome virus (wssv) at 12 h. fclegu mrna in hepatopancreas was down-regulated by vibrio. fclegu transcript first declined from 2 h to 6 h and then recov ...201020362060
a real-time pcr for the detection of hepatopancreatic parvovirus (hpv) of penaeid shrimp.hepatopancreatic parvovirus (hpv) causes a common shrimp disease that occurs in many shrimp farming regions, especially in the indo pacific, and infects most of the cultured penaeid species. there are seven geographic hpv isolates known, so a method to detect different hpv types is needed. we developed a sensitive and generic real-time pcr assay for the detection of hpv. a pair of primers and taqman probe based on an hpv sequence obtained from samples of fenneropenaeus chinensis from korea were ...201020367743
molecular cloning of daphnia magna catalase and its biomarker potential against oxidative stresses.catalase (ec 1.11.1.6) is an important antioxidant enzyme that protects aerobic organisms against oxidative damage by degrading hydrogen peroxide to oxygen and water. catalase mrnas have been cloned from many species and employed as useful biomarkers of oxidative stress. in the present study, we cloned the cdna from the catalase gene in daphnia magna, analyzed its catalytic properties, and investigated mrna expression patterns after the exposure to known oxidative stressors. the catalase proxima ...201020470898
screening of genes related to ovary development in chinese shrimp fenneropenaeus chinensis by suppression subtractive hybridization.the ovary of triploid shrimp fenneropenaeus chinensis was apparently impaired compared to that of the diploid shrimp at the same age. therefore triploid shrimp ovary is possible to be taken as a model to understand the mechanism of ovary development of shrimp compared to that of the ovary of diploid shrimp at the same age. in the present study, a suppression subtractive hybridization (ssh) technique was applied to identify differentially expressed genes in the ovary between diploid and triploid ...201020403774
identification of three different types of serine proteases (one sp and two sphs) in chinese white shrimp.serine proteases (sps) and serine protease homologs (sphs) participate in digestion, embryonic development, blood coagulation, and immune defense responses. in this paper, we identify one sp and two sphs, including a masquerade sph (fcmas), a cub domain containing sp (fccubsp), and a single domain containing sph (fcsph2) in chinese white shrimp, fenneropenaeus chinensis. fcmas has a gly-rich region formed by three repeats of lggqggg, a clip domain and a c-terminal sp-like domain. absence of ser ...201021109005
molecular cloning and characterization of three crustins from the chinese white shrimp, fenneropenaeus chinensis.antibacterial peptides crustins are the effector molecules of innate immunity in decapods. in this study, three crustin cdna sequences (fc-crus 1, fc-crus 2, and fc-crus 3) were cloned from the chinese white shrimp fenneropenaeus chinensis. the full-length cdnas of fc-crus 2 and 3 are 473 bp and 574 bp, respectively. the deduced peptides of fc-crus 2 and 3 contain a signal peptide and a crustin domain at the c-terminal formed by twelve conserved cysteine residues. the partial sequence of fc-cru ...201020036745
a c-type lectin is involved in the innate immune response of chinese white shrimp.c-type lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this work, a cdna for a new c-type lectin, fclec3, was obtained from chinese white shrimp fenneropenaeus chinensis using expressed sequence tag analysis and rapid amplification of the cdna ends. fclec3 contains an n-terminal signal peptide and a carbohydrate recognition domain (crd). rt-pcr analysis showed that fclec3 was mainly expressed in hepatopancreas and that the expression ...200919647083
an acyl-coa-binding protein (fcacbp) and a fatty acid binding protein (fcfabp) respond to microbial infection in chinese white shrimp, fenneropenaeus chinensis.acyl-coa-binding protein (acbp) and fatty acid-binding protein (fabp) are involved in lipid metabolism. acbp plays a key role in multiple cellular tasks including modulation of fatty acid biosynthesis, enzyme regulation, vesicular trafficking, and gene regulation. in our study, a 536 bp cdna of acbp (fcacbp) was cloned and identified as a widely distributed gene in the chinese white shrimp, fenneropenaeus chinensis. its expression in intestine was upregulated in response to white spot syndrome v ...200919766195
a novel c-type lectin (fclec4) facilitates the clearance of vibrio anguillarum in vivo in chinese white shrimp.c-type lectins play important roles in innate immunity of invertebrates. in the present study, we report a novel c-type lectin, named fclec4, from the chinese white shrimp fenneropenaeus chinensis. fclec4 contains a single carbohydrate recognition domain (crd) with a putative signal peptide. phylogenetic analysis indicated that fclec4 was distant from most reported c-type lectins from shrimps. the expression of fclec4 increased at both mrna and protein level after stimulation of vibrio anguillar ...200919447130
identification and molecular characterization of a spätzle-like protein from chinese shrimp (fenneropenaeus chinensis).in invertebrates, the toll signaling pathway is important for activation of antimicrobial peptides in the innate immune system. activation of the toll pathway requires binding of toll with its ligand spätzle. here we described a spätzle-like protein, designated as fc-spz, from hemocytes of chinese shrimp, fenneropenaeus chinensis. the deduced amino acid sequence of fc-spz shares 54% identity with spätzle-like protein of salmon louse (lepeophtheirus salmonis). semi-quantitative reverse transcript ...200919616633
cloning and expression of glucose regulated protein 78 (grp78) in fenneropenaeus chinensis.grp78 (78 kda glucose-regulated protein), also known as bip (immunoglobulin heavy-chain-binding protein), is an essential regulator of endoplasmic reticulum (er) homeostasis because of its multiple functions in protein folding, er calcium binding, and controlling of the activation of transmembrane er stress sensors. in this report, we cloned the full length cdna of grp78 (fcgrp78) from chinese shrimp fenneropenaeus chinensis. this cdna revealed a 2,325 bp with 1,968 bp open reading frame encodin ...200918038268
molecular cloning and characterisation of a pattern recognition protein, lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) from chinese shrimp fenneropenaeus chinensis.a pattern recognition protein (prp), lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte of chinese shrimp fenneropenaeus chinensis by the techniques of homology cloning and race. analysis of nucleotide sequence revealed that the full-length cdna of 1,275 bp has an open reading frame of 1,098 bp encoding a protein of 366 amino acids including a 17 amino acid signal peptide. sequence comparison of the deduced amino acid sequence of f. chinensis lgbp sh ...200918163220
molecular cloning and characterisation of prophenoloxidase (propo) cdna from fenneropenaeus chinensis and its transcription injected by vibrio anguillarum.the prophenoloxidase(propo) gene was cloned from haemocytes of chinese shrimp fenneropenaeus chinensis by rapid amplification complementary dna ends (race) method. the full-length cdna of prophenoloxidase gene consists of 3040 bp with a 2061 bp open reading frame (orf), encoding 686 amino acids. phylogenetic analysis revealed that it belongs to insect-type invertebrate prophenoloxidase gene family. to understand propo reaction for pathogeny's challenge in shrimp, the expressions of propo in diff ...200918595005
a serpin from chinese shrimp fenneropenaeus chinensis is responsive to bacteria and wssv challenge.arthropod defence responses (e.g. prophenoloxidase (propo) activation and toll pathway initiation) are mediated by serine proteinase cascades and regulated by serpins in haemolymph. a serpin (fc-serpin) cdna was cloned from the haemocytes of fenneropenaeus chinensis by rapid amplification of cdna ends (race) pcr and haemocyte cdna library screening. the full-length cdna consists of 1734bp, encoding 411 amino acids with a calculated molecular mass of 46.55kda and a theoretical isoelectric point o ...200918817877
molecular cloning and characterization of the translationally controlled tumor protein from fenneropenaeus chinensis.the translationally controlled tumor protein (tctp) is a multi-functioning protein that performs vital roles, particularly in various complicated life processes. in this study, a new tctp cdna was cloned from fenneropenaeus chinensis and hence was designated as fc-tctp. its length is 711 bp, and it is characterized by 507-bp open reading frame that encodes a deduced 168-amino acid protein, including a tctp domain. moreover, this study analyzed the expression patterns of this gene when it respond ...200918853281
clip domain serine protease and its homolog respond to vibrio challenge in chinese white shrimp, fenneropenaeus chinensis.clip domain serine proteases and their homologs are involved in invertebrate innate immunity, including hemolymph coagulation, antimicrobial peptide synthesis, cell adhesion, and melanization. recognition of pathogens by pattern recognition receptors can trigger activation of a serine protease cascade. we report here the cdna cloning of a serine protease (fcsp) and a serine protease homolog (fcsph) from chinese white shrimp, fenneropenaeus chinensis. both fcsp and fcsph possess a clip domain at ...200919332134
a selenium-dependent glutathione peroxidase (se-gpx) and two glutathione s-transferases (gsts) from chinese shrimp (fenneropenaeus chinensis).glutathione s-transferases (gsts) and glutathione peroxidases (gpxs) are essential components of cellular detoxification systems that defend cells against reactive oxygen species (ross). two gstgenes have recently been cloned from fenneropenaeus chinensis and blast p analysis shows that one gst, designated fcmugst, is similar to members of mugst while the other has similarities to thetagst (fcthetagst). a selenium-dependent glutathione peroxidase (se-gpx) has also been cloned from f. chinensis. ...200919358336
trbp homolog interacts with eukaryotic initiation factor 6 (eif6) in fenneropenaeus chinensis.the hiv transactivating response rna-binding protein (trbp) plays an important role in many biological processes. we have cloned three cdnas from newly identified genes in the trbp family from fenneropenaeus chinensis. these genes have been designated fc-trbp1-3. recombinant fc-trbp1, which was produced in escherichia coli, was used for panning of a t7 phage display library of the chinese shrimp hemocytes. from this panning, fc-eukaryotic initiation factor 6 (fc-eif6) was isolated and sequenced. ...200919380771
identification of a novel relish homolog in chinese shrimp fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides.penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. antimicrobial peptides (amp) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the shrimp amp gene expression is still absent up to date. in the current study, a relish homolog (fcrelish) was cloned from chinese shrimp fenneropenaeus chinensis. the full length cd ...200919520110
molecular cloning and characterization of the lipopolysaccharide and beta-1, 3-glucan binding protein in chinese mitten crab (eriocheir sinensis).the lipopolysaccharide and beta-1, 3-glucan binding protein (lgbp) is a pattern recognition protein which is fundamental for the innate immune response of crustaceans. a partial cdna produced by the random sequencing of cdna clones from a haemocyte cdna library of eriocheir sinensis showed similarity to the lgbp gene of the chinese shrimp (fenneropenaeus chinensis). the full-length cdna was subsequently cloned and sequenced by the technique of rapid amplification of cdna ends (race). the e. sine ...200919426826
molecular cloning of kazal-type proteinase inhibitor of the shrimp fenneropenaeus chinensis.proteinase inhibitors play important roles in host defence systems involving blood coagulation and pathogen digestion. we isolated and characterized a cdna clone for a kazal-type proteinase inhibitor (kpi) from a hemocyte cdna library of the oriental white shrimp fenneropenaeus chinensis. the kpi gene consists of three exons and two introns. kpi cdna contains an open reading frame of 396 bp, a polyadenylation signal sequence aataaa, and a poly (a) tail. kpi cdna encodes a polypeptide of 131 amin ...200919041263
cdna cloning and gene expression pattern following bacterial challenge of peroxinectin in chinese shrimp fenneropenaeus chinensis.peroxinectin, a cell-adhesive hemoperoxidase that binds superoxide dismutase and mediates blood cells adhesion and migration in invertebrate, is believed to play an important role in cellular immune reaction. in this study, we reported a new peroxinectin gene homologue from chinese shrimp fenneropenaeus chinensis. based on expressed sequence tags (ests) of haemocyte cdna library, we cloned a 2,611 bps full-length cdna of peroxinectin gene homologue encoded 801 amino acids. motif scanning of the ...200919148772
expression of four trypsin-like serine proteases from the chinese shrimp, fenneropenaeus chinensis, as regulated by pathogenic infection.four trypsin-like serine proteases, designated as fctry1, fctry2, fctry3, and fctry4, were obtained from the hepatopancreas of the chinese shrimp, fenneropenaeus chinensis. three trypsin-like serine proteases, specifically fctry1, 2, and 3 were observed to have the conserved catalytic triad (h, d, and s). regarding fctry4, the catalytic triad s was substituted by f, and the mature peptide was found to be inactive. further analysis for similarities indicated that fctry1, 2 and 4 were 89-92% ident ...200919416700
a double wap domain (dwd)-containing protein with proteinase inhibitory activity in chinese white shrimp, fenneropenaeus chinensis.the whey major component, whey acidic protein (wap), has one or more wap domains characterized by a four-disulfide core (4-dsc) structure. these kinds of proteins are involved in multiple functions, including proteinase inhibitor activity, antimicrobial activity, atpase inhibitor activity, and regulatory function in cell proliferation. recent research indicates that wap domain-containing proteins play an important role in the innate immunity of crustaceans. in this study, a novel double wap doma ...200919545640
cloning of profilin (fcpfn) from the shrimp fenneropenaeus chinensis, a highly expressed protein in white spot syndrome virus (wssv)-infected shrimp.we isolated and characterized the profilin (fcpfn) cdna from hemocytes of fenneropenaeus chinensis, a unique shrimp species from the yellow sea. the fcpfn cdna consists of 830 bp and encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.06. the deduced amino acid sequence of fcpfn shows 36% and 90% amino acid sequence identity to the profilin genes of pacific white shrimp litopenaeus vannamei and black tiger shrimp penaeus monodon, respectively. the fcpfn mrna was h ...200919638679
neutralization of white spot syndrome virus (wssv) for penaeus chinensis by antiserum raised against recombinant vp19.this study was carried out to neutralize the wssv one of the most virulent pathogen causing large economic damage in shrimp culture industry using the antiserum produced against recombinant wssv envelope protein vp19 (rvp19) as a tool to evaluate wssv infection mechanism. a fragment of vp19 was expressed in sf21 insect cell using baculovirus expression system as fusion protein with 6 his-tag. then, polyclonal antiserum against rvp19 was raised in white rabbit. a constant amount of wssv (at 10(4) ...200819195390
vaccination of shrimp (penaeus chinensis) against white spot syndrome virus (wssv).two structural protein genes, vp19 and vp466, of white spot syndrome virus (wssv) were cloned and expressed in sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against wssv for shrimps. the cumulative mortalities of the shrimps vaccinated by the injection of rvp19 and rvp466 at 15 days after the challenge with wssv were 50.2% and 51.8%, respectively. for the vaccination by oral feeding of rvp19 and rvp466, the cumulative mortaliti ...200818633299
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