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detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time pcr assay.as one of the major pathogens, hepatopancreatic parvovirus (hpv) can cause severe diseases in penaeid shrimp. we developed a taqman-based real-time pcr assay for the hpv detection in china. a pair of primers (hpvf and hpvr) and a taqman probe were designed according to the hpv genomic sequence of chinese isolate (genbank: gu371276). our data showed that the primers and taqman probe were specific for hpv, and they exhibited no cross-reaction with infectious hypodermal and hematopoietic necrosis v ...201324090693
molecular cloning and characterization of a cathepsin b gene from the chinese shrimp fenneropenaeus chinensis.cathepsin b is a unique member of the cathepsin superfamily, which acts as both an endopeptidase and peptidyl-dipeptidase. to obtain a better understanding of this enzyme, we cloned a cdna encoding cathepsin b from the muscle of fenneropenaeus chinensis (fccb). fccb contained a 996-bp open reading frame (orf) encoding a protein of 331 amino acid residues with a putative signal peptide and a propeptide_c1 at the n-terminal, a glutamine oxyanion hole and active site cysteine, histidine and asparag ...201324041842
transcriptome analysis on chinese shrimp fenneropenaeus chinensis during wssv acute infection.previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (wssv) infection in crustacean. however, little information is available in relation to underlying mechanisms of host responses during the wssv acute infection stage in naturally infected shrimp. in this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. a tot ...201323527000
identification, cloning and characterization of an extracellular signal-regulated kinase (erk) from chinese shrimp, fenneropenaeus chinensis.extracellular signal-regulated kinase (erk) is a serine/threonine-specific protein kinase, which participates in signaling transduction pathways that control intracellular events, including resumption of meiosis, embryogenesis, cell differentiation, cell proliferation, cell death and response to radiation. some virus species evolved the ability to hijack the host cell erk signaling transduction pathway for viral replications and gene expressions. to obtain a better understanding of erk, we clone ...201324084041
identification and characterization of a serine protease inhibitor esserpin from the chinese mitten crab eriocheir sinensis.serine protease inhibitors (serpins) represent an expanding superfamily of endogenous inhibitors that regulate proteolytic events and involve in a variety of physiological processes. a serine protease inhibitor, namely esserpin, was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) analysis. the full-length cdna of esserpin was of 2367 bp, including an open reading frame (orf) of 1371 bp encoding a polypeptide of 456 amino acids with estimated molecular ...201323567854
shrimp myd88 responsive to bacteria and white spot syndrome virus.the myeloid differentiation factor 88 (myd88) is an important adapter protein which links members of the toll-like receptor (tlr) to the downstream components to activate related signaling pathways. in the present study, a myd88 homolog (fcmyd88) was cloned from penaeid shrimp fenneropenaeus chinensis. the orf of fcmyd88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (dd) and a typical tlr and interleukin-1 receptor (il-1r)-related (tir) domain. homo ...201323238389
characterization of an immune deficiency homolog (imd) in shrimp (fenneropenaeus chinensis) and crayfish (procambarus clarkii).the immune deficiency (imd) signal pathway mediates immunity against gram-negative bacteria in drosophila. recent studies show that the imd pathway also involves in antiviral innate immune responses. the functions of the pathway in crustacean immunity are largely unknown. in this paper, two imds (fcimd and pcimd), one of the key elements of the imd pathway, were identified from chinese white shrimp fenneropenaeus chinensis and red swamp crayfish procambarus clarkii. both proteins have a death do ...201323850721
characterization and function analysis of an anti-lipopolysaccharide factor (alf) from the chinese shrimp fenneropenaeus chinensis.anti-lipopolysaccharide factor (alf) is one of the widely-studied antimicrobial peptides (amps) with broad-spectrum antibacterial activity and antiviral property. previous studies show the existence of multiform of alfs in crustacean which are important for immunity of the animals. in the present study, we characterized one isoform of alf from the chinese shrimp fenneropenaeus chinensis (fcalf2). tissue distribution analysis revealed that fcalf2 showed the highest expression level in the lymphoi ...201424879940
functional characterization of a c-type lysozyme from indian shrimp fenneropenaeus indicus.lysozyme gene from fenneropenaeus indicus was cloned, expressed in escherichia coli and characterized. the cdna consists of 477 base pairs and encodes amino acid sequence of 159 residues. f. indicus lysozyme had high identity (98%) with fenneropenaeus merguiensis and fenneropenaeus chinensis and exhibits low to moderate identities with lysozymes of other invertebrates and vertebrates. this lysozyme is presumed to be chicken types as it possesses two catalytic and eight cysteine residues that are ...201424676722
comparison of protein expression profiles of the hepatopancreas in fenneropenaeus chinensis challenged with heat-inactivated vibrio anguillarum and white spot syndrome virus.fenneropenaeus chinensis (chinese shrimp) culture industry, like other penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. to better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. firstly, the soluble hepatopancreas protein samples in adult chinese shrimp among control, heat-inactivated vibrio-challenged and white spot syndrome virus-infected groups were separated ...201424057166
molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from oriental river prawn, macrobrachium nipponense.the lipopolysaccharide and β-1,3-glucan binding protein (lgbp), one of the pattern recognition proteins, plays an important role in the innate immune response of invertebrates. a 1,506 bp full-length cdna of a lgbp gene was cloned and characterized from the oriental river prawn macrobrachium nipponense (named as mnlgbp). analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,119 bp, encoding a protein of 372 amino acids including a 21-aa signal peptide. t ...201424584659
assessment of microwave cooking on the bioaccessibility of cadmium from various food matrices using an in vitro digestion model.bioaccessibility represents the maximum amount of pollutant ingested with food that is available for intestinal absorption. the measurement of bioaccessibility can achieve a more accurate risk assessment. thus, in this study, the bioaccessibility of raw/microwave-cooked store-bought food including carrot, potato, white radish, lotus root, sweet corn, long grain rice, soybean, fleshy prawn, eastern oyster, kelp, and common carp were investigated by applying an in vitro digestion method. a validat ...201424958019
cloning and characterization of cytoplasmic dynein intermediate chain in fenneropenaeus chinensis and its essential role in white spot syndrome virus infection.to investigate the role of cytoplasmic dynein in white spot syndrome virus (wssv) infection, the full-length cdna of cytoplasmic dynein intermediate chain (fcdynci) was cloned in fenneropenaeus chinensis, which consists of 2582 bp and encodes a polypeptide of 660 amino acids. sequence analysis and multiple sequence alignment displayed that fcdynci was a member of cytoplasmic dynein 1 family. the fcdynci mrna was most highly expressed in hemocytes, which was significantly up-regulated post wssv i ...201424925758
a new anti-lipopolysaccharide factor (alf) gene with its snp polymorphisms related to wssv-resistance of litopenaeus vannamei.anti-lipopolysaccharide factors (alfs) of crustacean play an important role against bacteria or virus infection. in this study, the cdna sequence and genomic sequence of one new isoform of alf designated as nlvalf1 were reported. the open reading frame (orf) of nlvalf1 consisted of 369 bp encoding 123 amino acids and the genomic structure of nlvalf1 comprised four introns and three exons. the predicted pi of the deduced protein was 8.82 and the molecular weight (mw) was 13.72 kda. the deduced am ...201424769128
cloning of hsp21 gene and its expression in chinese shrimp fenneropenaeus chinensis in response to wssv challenge.in the present study, a dna sequence encoding a small heat shock protein gene (fchsp21) in the chinese shrimp, fenneropenaeus chinensis, was cloned, and its expression was analyzed after white spot syndrome virus (wssv) infection. the fchsp21 gene contained an open reading frame (orf) of 555 bp in length, encoding a 184 amino acid protein with a theoretical size of about 21 kda and a predicted isoelectric point of 5.38. the mrna of the hsp21 had a long poly(a) tail (748 bp) with six polyadenylat ...201424442516
molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in fenneropenaeus chinensis and its antioxidant activity.peroxiredoxins (prx) are a family of antioxidant proteins and perform important functions in intracellular signal transduction. here, we report a prx gene from chinese shrimp fenneropenaeus chinensis. the full-length cdna of fcprx gene contained an open reading frame of 735 bp encoding a polypeptide of 275 amino acids. the molecular mass of the deduced amino acid of fcprx is 27445.43 da with an estimated pi of 5.71. sequence comparison showed that the fcprx shares high identities with prx ivs an ...201424418456
full-length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the indian white prawn, fenneropenaeus indicus (h. milne edwards).elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture-reared shrimps is pivotal for the prevention and/or control of disease outbreaks. in this study, we examined the phylogenetic relatedness and compared the possible immune-related functional roles of both translationally controlled tumour protein (tctp) and ferritin genes with previous studies. both tctp and ferritin genes were substantially upregulated in the indian white prawn, fenneropenaeus ...201424329985
a new nodavirus is associated with covert mortality disease of shrimp.a new nodavirus, named covert mortality nodavirus (cmnv), is associated with covert mortality disease of shrimp which has caused serious loss in china since 2009. histopathological examination of shrimp suffering the disease revealed coagulative necrosis of striated muscle similar to typical histopathology features of infectious myonecrosis virus (imnv), penaeus vannamei nodavirus (pvnv) and macrobrachium rosenbergii nodavirus (mrnv). however, shrimp suffering this disease tested negative for im ...201425205685
proteomic analysis of differentially expressed proteins in fenneropenaeus chinensis hemocytes upon white spot syndrome virus infection.to elucidate molecular responses of shrimp hemocytes to white spot syndrome virus (wssv) infection, two-dimensional gel electrophoresis was applied to investigate differentially expressed proteins in hemocytes of chinese shrimp (fenneropenaeus chinensis) at 24 h post infection (hpi). approximately 580 protein spots were detected in hemocytes of healthy and wssv-infected shrimps. quantitative intensity analysis revealed 26 protein spots were significantly up-regulated, and 19 spots were significa ...201424587154
genomic organization and promoter analysis of a transcriptional repressor gene from fenneropenaeus chinensis.in this study, we cloned and sequenced genomic sequences from a fenneropenaeus chinensis transcriptional repressor gene, fctr. the fctr gene is 2,671 bp in length and has four exons and three introns. the 873 bp promoter contains several transcription factor binding sites, including a tata box and a cyclic amp-responsive element. promoter deletion analysis using a luciferase reporter gene identified regulatory elements. challenge with white spot syndrome virus increased expression from the promo ...201525266240
recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (fcalf5) from chinese shrimp fenneropenaeus chinensis.antimicrobial peptides (amps) have a great potential to be used as a substitute for antibiotics since amps don't lead to bacteria's drug resistance. anti-lipopolysaccharide factors (alfs) are one type of amps and exist in crustaceans. in the present study, we produced a recombinant protein (rfcalf5) of an alf isoform (fcalf5) from chinese shrimp fenneropenaeus chinensis through a prokaryotic expression system. the rfcalf5 exhibited varied antibacterial activities against different bacteria. besi ...201526123888
cloning and characterization of calreticulin and its association with salinity stress in p. trituberculatus.calreticulin (crt) is a highly conserved and multifunctional endoplasmic reticulum (er) chaperone protein and plays important roles in salinity stress response. portunus trituberculatus is a commercially important fishery species, and water salinity conditions influence its commercial farming significantly. in order to research the function of calreticulin under salinity stress, the full-length cdna sequence of calreticulin from p. trituberculatus (ptcrt) was firstly cloned and characterized. th ...201525995067
recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis.anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, w ...201627517939
sumo and sumo-conjugating enzyme e2 ubc9 are involved in white spot syndrome virus infection in fenneropenaeus chinensis.in previous work, small ubiquitin-like modifier (sumo) in hemocytes of chinese shrimp fenneropenaeus chinensis was found to be up-regulated post-white spot syndrome virus (wssv) infection using proteomic approach. however, the role of sumo in viral infection is still unclear. in the present work, full length cdnas of sumo (fcsumo) and sumo-conjugating enzyme e2 ubc9 (fcubc9) were cloned from f. chinensis using rapid amplification of cdna ends approach. the open reading frame (orf) of fcsumo enco ...201626927328
a novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (yhv-8) of yellow head virus.a new genotype of yellow head virus (yhv), designated as yhv-8, was found in farmed shrimp fenneropenaeus chinensis suffering suspectedly from ems/ahpns (early mortality disease/acute hepatopancreatic necrosis disease) in china in 2012. in this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rrt-lamp) assay was developed for better detection of both genotypes of yhv-1 and yhv-8. a set of six specific primers was successfully designed targeting a conserv ...201627221155
comparative microarray profile of the hepatopancreas in the response of "huanghai no. 2" fenneropenaeus chinensis to white spot syndrome virus.white spot syndrome virus (wssv) infects all shrimp species and is the greatest detriment to shrimp culture. to better understand the mechanism of molecular responses to wssv infection in "huanghai no. 2" fenneropenaeus chinensis, a microarray technique was used. microarray gene expression profiling of 59,137 unigenes identified differentially expressed genes (degs) both in live and moribund shrimp at early, peak and late phases. in live shrimp, 1307, 1479 and 1539 degs were obtained in the earl ...201627591045
dietary supplementation of probiotic bacillus pc465 isolated from the gut of fenneropenaeus chinensis improves the health status and resistance of litopenaeus vannamei against white spot syndrome virus.this study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic bacillus pc465 on the growth, health status, and disease resistance of litopenaeus vannamei. shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) cfu g(-1)]. probiotic supplementation significantly increased the weight gain and survival of l. vannamei (p < 0.05). the effect of ...201627177431
cloning of litopenaeus vannamei cd63 and it's role in white spot syndrome virus infection.white spot syndrome virus (wssv) is currently the most serious shrimp pathogen, which has brought huge losses to shrimp industry worldwide. cd63 of shrimp belongs to the tetraspanin superfamily, which plays an important role in signal transduction and immune process. in this paper, cd63 cdna sequence of litopenaeus vannamei was cloned using race method. the amplified sequence is 1472 bp, with its orf 744 bp, encoding 247 amino acids. bioinformatics analysis showed that the sequence of lvcd63 has ...201626964710
pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of fenneropenaeus chinensis farming pond.a novel aerobic bacterium, gram-stain-negative, motile, oxidase- and catalase-positive, non-spore-forming, non-pigmented, designated rzy34t, was isolated from the sediment of fenneropenaeus chinensis farming pond, rizhao, china. the strain was able to grow at ph 6-10 (optimum ph 7), 20-40 °c (optimum 30 °c) and in the presence of 1.0-6.0% nacl (optimum 1.0-2.0%). phylogenetic analysis based on 16s rrna gene sequences revealed that the nearest relative of strain rzy34t was pseudoalteromonas xiame ...201627149865
virus-derived small rnas in the penaeid shrimp fenneropenaeus chinensis during acute infection of the dna virus wssv.small interfering rnas (sirnas) and micrornas (mirnas) are two classes of small rnas (srnas) that are critical for virus-host interplay via the rna interference (rnai) pathway. one virus-derived sirna and numerous mirnas has been reported for the double-stranded dna virus white spot syndrome virus (wssv), however, the expression profiles of these different types of srnas have not been assessed. here, by sequencing the srnas and mrnas of wssv-infected chinese shrimp (fenneropenaeus chinensis), we ...201627349643
isolation and expression analysis of an mapkk gene from fenneropenaeus chinensis in response to white spot syndrome virus infection.mitogen-activated kinase kinase (mapkk) is an important gene involved in the host-virus interaction process. to obtain a better understanding of mapkk in the interaction process between the chinese shrimp fenneropenaeus chinensis and white spot syndrome virus (wssv), we cloned the sequence of an mapkk cdna from f. chinensis (fcmapkk) and investigated the effect of fcmapkk on wssv infection. the results showed that the fcmapkk gene contained a 1227 bp open reading frame (orf), which encoded a hig ...201627164214
haemocyanin content of shrimp (fenneropenaeus chinensis) associated with white spot syndrome virus and vibrio harveyi infection process.haemocyanin (hc) is frequently reported to vary significantly by physiological status and environmental stress in crustaceans. in this paper, the shrimp fenneropenaeus chinensis was infected with different concentrations of white spot syndrome virus (wssv) and vibrio harveyi. then, the variation of hc and total protein content of the haemolymph (tpch) were investigated using the established double antibody sandwich enzyme linked immunosorbent assay (das-elisa) and coomassie brilliant blue method ...201626616234
complete genome sequence of an isolate of a novel genotype of yellow head virus from fenneropenaeus chinensis indigenous in china.genotype 8 of yellow head virus (yhv-8) was identified recently, but the complete genome sequence of this new genotype has not been reported. in this study, the complete genome of yhv-8 isolate 20120706 collected from hebei province of china in 2012 was sequenced. it was found to be 26,769 nucleotides (nt) in length, including a 20,060-nt open reading frame 1 (orf1), a 435-nt orf2, and a 4971-nt orf3. isolate 20120706 shared 79.7-83.9% nucleotide sequence identity with all seven of the complete ...201728044194
the identification of micrornas involved in the response of chinese shrimp fenneropenaeus chinensis to white spot syndrome virus infection.microrna (mirna) is a class of small noncoding rna, which is involved in the post-transcriptional regulation in all metazoan eukaryotes. mirnas might play an important role in the host response to virus infection. however, mirnas in the aquatic crustacean species were not extensively investigated. to obtain a better understanding of the response of chinese shrimp fenneropenaeus chinensis to white spot syndrome virus (wssv) infection, the sequence and expression profile of mirnas in the hepatopan ...201728554838
cdna cloning and expression analysis of a phosphopyruvate hydratase gene from the chinese shrimp fenneropenaeus chinensis.in the present study a cdna encoding a phosphopyruvate hydratase (enolase) was cloned from the muscle of the chinese shrimp (fenneropenaeus chinensis) and named as fcenolase. the cdna of fcenolase encoded a protein of 434 amino acid residues with a molecular mass 47.22 kda. the residues 342-355 constituted the signature motif "lllkvnqigsvtes". a snp locus (c96t) in the orf at 96 bp was identified. the results showed that the fcenolase was a conserved gene. in the normal f. chinensis, the mrna le ...201728216323
the roles of β-integrin of chinese shrimp (fenneropenaeus chinensis) in wssv infection.our previous study demonstrated that an integrin β subunit of chinese shrimp (fenneropenaeus chinensis) (fcβint) plays an important role in white spot syndrome virus (wssv) infection. in the present work, in order to further elucidate the potential role of fcβint in wssv infection, the recombinant extracellular domain of β integringene of f. chinensis (rfcβint-er) was expressed in escherichia coli bl21 (de3), and the eukaryotic expression plasmid pcdna3.1-fcβint-er (pfcβint-er) was also construc ...201728686185
expression profiles of the p38 mapk signaling pathway from chinese shrimp fenneropenaeus chinensis in response to viral and bacterial infections.mitogen-activated protein kinase (mapk) signaling pathway plays an important role in cellular response to inflammatory cytokines, environmental stress and pathogenic infection, however, compared with mammals and insects, the potential function of p38 mapk from chinese shrimp (fenneropenaeus chinensis) in response to viruses and bacterial infection is limited. in the study, the immune responses of four genes mkk3, mkk4, p38 and atf-2 from f. chinensis were investigated in defending against white ...201829155327
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