Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| a non-destructive method based on the polymerase chain reaction for detection of hepatopancreatic parvovirus (hpv) of penaeid shrimp. | current methods to detect hepatopancreatic parvovirus (hpv) infection of penaeid shrimp depend on invasive techniques that require dissecting the organs infected by this virus. however, sacrificing valuable stocks in order to determine their hpv status can be a drawback in the case of breeding programs. a method was developed for hpv detection by applying a polymerase chain reaction (pcr) assay to fecal samples collected from live hpv-infected shrimp penaeus chinensis. a pair of pcr primers, 112 ... | 2000 | 10768285 |
| different reactions obtained using the same dna detection reagents for thai and korean hepatopancreatic parvovirus of penaeid shrimp. | hepatopancreatic parvovirus (hpv) can cause stunted growth and death in penaeid shrimp including penaeus monodon. we used pcr primers and a commercial dna probe designed from hpv of penaeus chinensis (hpvchin) to examine hpv-infected thai p. monodon (hpvmon). we found that the pcr primers produced a 732 bp dna amplicon rather than the 350 bp amplicon obtained with hpvchin template and that the dna probe gave weak to variable in situ dna hybridization results. in addition, hybridization to pcr pr ... | 2001 | 11678231 |
| [variation of bacteria numbers in fish-shrimp mix-culturing ecosystem]. | the study on variation of bacteria numbers in penaeus chinensis-fish mix-culturing ecosystem in 1997. indicated that at the beginning of culturing season, total number of heterotrophic bacteria and that of nitrate-reducing bacteria in mix-culturing ponds was low, but it was higher than that in mono-culturing shrimp pond. with time going on, the number of bacteria in mono-culturing pond increased rapidly and remained at a high level in august and september, an that in mix-culturing ponds also inc ... | 2002 | 12216404 |
| detection of hepatopancreatic parvovirus in thai shrimp penaeus monodon by in situ hybridization, dot blot hybridization and pcr amplification. | hepatopancreatic parvovirus (hpv) infects the hepatopancreas in penaeid shrimp and retards their growth. the dna sequence of hpv from thai shrimp penaeus monodon (hpvmon) differs from hpv of penaeus chinensis (hpvchin) by approximately 30%. in spite of this difference, commercial pcr primers (diagxotics) developed from hpvchin to yield a 350 bp pcr product do give a 732 bp product with hpvmon dna template. on the other hand, the sensitivity of hpvmon detection with these primers and with hybridi ... | 2002 | 12465880 |
| [infection of white spot baculo-like virus(wsbv) in penaeus chinensis: evidence from electron microscopy and dna hybridization]. | severe epizootic causing high mortalities in cultivated penaeus chinensis has occurred every year in mainland china since 1993 with great economic loss. naturally diseased p. chinensis sampled in qingdao shrimp farming regions between 1993 and 1998 were examined by electron microscopy. a similar non-occluded nuclear bacilliform virus like the white spot baculo-like virus (wsbv) reported in taiwan was mostly found in the gill, cuticular epidermis under the exoskeleton, stomach, lymphoid organ and ... | 2000 | 12548988 |
| gonad development characteristics and sex ratio in triploid chinese shrimp ( fenneropenaeus chinensis). | this paper details for the first time the gonad development characteristics and sex ratio of triploid shrimp ( fenneropenaeus chinensis). in triploid shrimp the development of gonad is apparently impaired, especially in females. in the ovary of triploids, germ cells mainly remain at oogonia stage during september through december. from january to february of the next year, partial primary oocytes developed in the ovary lobes. spermatocytes and spermatids could be observed in the testes of triplo ... | 2003 | 14583810 |
| white spot syndrome virus (wssv) detected by pcr in rotifers and rotifer resting eggs from shrimp pond sediments. | white spot syndrome virus (wssv) was detected by pcr-dot blot hybridization in rotifer resting eggs from shrimp penaeus chinensis culture-pond sediments. it was also detected in rotifers hatched from those eggs. surface disinfection before analysis indicated that wssv was probably present within the resting eggs. results suggested that rotifer resting eggs may be an overwintering reservoir for wssv in shrimp ponds. | 2004 | 15212294 |
| phylogenetic relationships of 12 penaeoidea shrimp species deduced from mitochondrial dna sequences. | dna sequences of an 847 bp fragment of mitochondrial cytochrome oxidase subunit i (coi) gene and a 514 bp fragment of 16s rrna gene were determined to examine the phylogenetic relationships of 12 penaeoidea shrimp species (penaeus chinensis, penaeus japonicus, penaeus penicillatus, penaeus vannamei, penaeus canaliculatus, trachypenaeus curvirostris, metapenaeus affinis, metapenaeus ensis, metapenaeopsis barbata, parapenaeus fissuroides, parapenaeopsis hardiwickii, solenocera crassicomis). both f ... | 2004 | 15524311 |
| molecular cloning and response to laminarin stimulation of arginine kinase in haemolymph in chinese shrimp, fenneropenaeus chinensis. | arginine kinase (ak) was previously reported as a phosphagen-atp phosphotransferase found in invertebrates. in this study, an 1184 bp cdna was cloned and sequenced. it contained an open reading frame of 1068 bp that coded for 356 deduced amino acids of ak in fenneropenaeus chinensis. the calculated molecular mass of ak is 40129.73 da and pi is 5.92. the predicted protein showed a high level of identity to known ak in invertebrates and creatine kinase from vertebrates, which belong to a conserved ... | 2005 | 15863013 |
| molecular cloning and expression profile of putative antilipopolysaccharide factor in chinese shrimp(fenneropenaeus chinensis). | a new antimicrobial protein gene of the anti-lipopolysaccharide factor family (tentatively named as alffc) has been cloned from hemocytes of the chinese shrimp fenneropenaeus chinensis by rapid amplification of 3' and 5' complementary dna ends with polymerase chain reaction. the full-length complementary dna of alffc consists of 600 bp with a 369-bp open reading frame, encoding 123 amino acids. the deduced peptide contains a putative signal peptide of 25 amino acids and mature peptide of 98 amin ... | 2005 | 16206014 |
| the microsatellites and minisatellites in the genome of fenneropenaeus chinensis. | through two-time sequencing randomly in fenneropenaeus chinensis, 2,597,000 bp cumulative length random genomic sequences about occupying 1.23 per thousand of the entire genome are obtained, in which the length of the first time sequencing is 884,000 bp, by cutting the genome dna with sau3ai enzyme, and the second is 1,713,000 bp by breaking the genome dna with the physical method, ultrasonic. using tandem repeat finder (trf) soft to analyze the sequences, 4,588 tandem repeats are found, in whic ... | 2005 | 16287622 |
| the effect of sargassum fusiforme polysaccharide extracts on vibriosis resistance and immune activity of the shrimp, fenneropenaeus chinensis. | immunostimulants are valuable for control of shrimp diseases and the immunostimulatory effects of some polysaccharide additives for shrimp have been reported. in this study, the sargassum fusiforme polysaccharide extract (sfpse) was assessed as a feed additive when supplemented in the diet (0%, 0.5%, 1.0%, and 2.0%) for juvenile shrimp, fenneropenaeus chinensis, in order to study the effects of sfpse on vibriosis resistance and immune activity. shrimp were cultured in the same pond with cages. t ... | 2006 | 16326114 |
| studies on nitric oxide synthase activity in haemocytes of shrimps fenneropenaeus chinensis and marsupenaeus japonicus after white spot syndrome virus infection. | the nitric oxide synthase (nos) activity in the haemocytes of shrimps fenneropenaeus chinensis (osbeck) and marsupenaeus japonicus (bate) was studied after white spot syndrome virus (wssv) infection to determine its characteristics in response to virus infection. first, the nos activity in haemocytes of shrimps was determined by the means of nbt reduction and changes in cell conformation. and the variations of nos activity in shrimps after challenge with wssv intramuscularly were evaluated throu ... | 2006 | 16403661 |
| molecular cloning, characterization and expression analysis of a putative c-type lectin (fclectin) gene in chinese shrimp fenneropenaeus chinensis. | lectin is regarded as a potential molecule involved in immune recognition and phagocytosis through opsonization in crustacean. knowledge on lectin at molecular level would help us to understand its regulation mechanism in crustacean immune system. a novel c-type lectin gene (fclectin) was cloned from hemocytes of chinese shrimp fenneropenaeus chinensis by 3' and 5' rapid amplification of cdna ends (race) pcr. the full-length cdna consists of 1482 bp with an 861 bp open reading frame, encoding 28 ... | 2007 | 16530268 |
| cloning and expression analysis of an o-methyltransferase (omt) gene from chinese shrimp, fenneropenaeus chinensis. | o-methyltransferase (omt) is ubiquitously present in diverse organisms and plays an important regulatory role in plant and animal growth, development, reproduction and defence and has also been implicated in human emotion and disease. a putative o-methyltransferase (omt) gene has been cloned from the haemocytes of bacteria-infected chinese shrimp (fenneropenaeus chinensis) by suppression subtractive hybridisation (ssh) coupled with the smart cdna method. the isolated 944 bp full-length cdna cont ... | 2006 | 16530427 |
| cloning, expression and identification of ferritin from chinese shrimp, fenneropenaeus chinensis. | ferritin, the iron storage protein, plays a key role in iron metabolism. a cdna encoding ferritin (fcfer) was cloned from hepatopancreas of chinese shrimp, fenneropenaeus chinensis. the predicted protein contains 170 amino acid residues with a predicted molecular weight (mw) about 19, 422.89 da and theoretical isoelectric point (pi) of 4.73. amino acid alignment of fcfer revealed 97% homology with litopenaeus vannamei ferritin. results of the rt-pcr showed that the expression of fcfer mrna was u ... | 2006 | 16621097 |
| discovery of the genes in response to white spot syndrome virus (wssv) infection in fenneropenaeus chinensis through cdna microarray. | we used microarray technology to study differentially expressed genes in white spot syndrome virus (wssv)-infected shrimp. a total of 3136 cdna targets, including 1578 unique genes from a cephalothorax cdna library and 1536 cdna clones from reverse and forward suppression subtractive hybridization (ssh) libraries of fenneropenaeus chinensis, plus 14 negative and 8 blank control clones, were spotted onto a 18 x 18 mm area of nh(2)-modified glass slides. gene expression patterns in the cephalothor ... | 2006 | 16794954 |
| expression and characterisation of tiger shrimp penaeus monodon penaeidin (mo-penaeidin) in various tissues, during early embryonic development and moulting stages. | a penaeidin family, mo-penaeidin was cloned from the haemocytes of tiger shrimp penaeus monodon using genomic polymerase chain reaction (pcr) by gene specific primers. analysis of nucleotide sequence revealed that this mo-penaeidin consists of 1348 bp containing one intron (680 bp) and two exons (210 and 458 bp). it has an open reading frame (orf) of 222 p, which encodes a protein of 74 amino acids including a signal peptide of 19 amino acids. the calculated molecular mass of the mature protein ... | 2007 | 16820207 |
| the mitochondrial manganese superoxide dismutase gene in chinese shrimp fenneropenaeus chinensis: cloning, distribution and expression. | manganese superoxide dismutase (mnsod) plays an important role in crustacean immune defense reaction by eliminating oxidative stress. knowledge on mnsod at molecular level allows us to understand its regulatory mechanism in crustacean immune system. a novel mitochondrial manganese superoxide dismutase (mmnsod) was cloned from hepatopancreas of chinese shrimp fenneropenaeus chinensis by 3' and 5' rapid amplification of cdna ends (race) pcr. the full-length cdna consists of 1185bp with a 660bp ope ... | 2007 | 17097141 |
| protection of shrimp (penaeus chinensis) against white spot syndrome virus (wssv) challenge by double-stranded rna. | to determine whether penaeus chinensis can be protected against white spot syndrome virus (wssv) infection by intramuscular injection with long double-stranded rnas (dsrnas) as in other shrimp species and whether the protection degree by wssv-specific dsrnas is correlated with the roles of viral genes, p. chinensis juveniles were intramuscularly injected with long dsrnas corresponding to vp28, vp281, protein kinase genes of wssv, and an unrelated long dsrna corresponding to a green fluorescence ... | 2007 | 17175172 |
| heterosis of haemolymph analytes of two geographic populations in chinese shrimp fenneropenaeus chinensis. | fenneropenaeus chinensis is distributed along the coasts of the northern seacoast of china and the korean peninsula. according to their migration routes, spawning and overwintering places, f. chinensis is divided mainly into three geographic variety populations. these populations represent potentially different genetic resources important to hybridisation breeding programs. to evaluate heterosis of the total protein concentration (pc), haemocyanin concentration (hc), penoloxidase (po) activity, ... | 2007 | 17215142 |
| a transglutaminase from chinese shrimp (fenneropenaeus chinensis), full-length cdna cloning, tissue localization and expression profile after challenge. | transglutaminase can catalyze the cross-linking reaction between soluble clotting protein molecules from the plasma for prevention of excess blood loss from a wound and obstructing micro-organisms from invading the wound in crustaceans. a novel transglutaminase (fctg) gene was cloned from hemocytes of chinese shrimp fenneropenaeus chinensis by 3' and 5' rapid amplification of cdna ends (race) pcr. the full-length cdna consists of 2972bp, encoding 757 amino acids with a calculated molecular mass ... | 2007 | 17218114 |
| expression, purification, and characterization of recombinant chinese shrimp crustin-like protein (crufc) in pichia pastoris. | a crustin-like protein (crufc) from fenneropenaeus chinensis was expressed in pichia pastoris and then purified to electrophoretic homogeneity on a sephacryl s-100 column with a band corresponding to the expected one (13 kda) shown by 15% sds-page. western blot indicated that the rcrufc specifically reacted with polyclonal rabbit anti-fenneropenaeus chinensis crufc. production in a 5 l bioreactor gave 237 mg rcrufc/l. antimicrobial assay revealed that 4 microm rcrufc inhibited growth of staphylo ... | 2007 | 17333464 |
| antilipopolysaccharide factor (alf) of mud crab scylla paramamosain: molecular cloning, genomic organization and the antimicrobial activity of its synthetic lps binding domain. | antilipopolysaccharide factors (alfs) are small basic proteins that can bind and neutralize lipopolysaccharide (lps) and have broad spectrum antimicrobial activities. in this study, we describe the isolation of the full-length cdna encoding for alf peptide (alfsp) of mud crab, scylla paramamosain by sequencing a hemocyte cdna library and using the rapid amplification cdna end (race) method. a full-length alfsp cdna of 614 bp contains an open reading frame (orf) of 372 bp, encoding 123 amino acid ... | 2007 | 17368541 |
| molecular characteristics and expression analysis of calreticulin in chinese shrimp fenneropenaeus chinensis. | calreticulin (crt), as an endoplasmic reticulum luminal resident protein, plays important roles in ca(2+) homeostasis and molecular chaperoning. crt on the surface of the cell can modulate cell adhesion, phagocytosis and integrin-dependent ca(2+) signaling. the full length cdna of calreticulin (fccrt) was cloned from chinese shrimp fenneropenaeus chinensis. it consists of 1672 bp with an open reading frame of 1221 bp, encoding 406 amino acids. this is the first reported cdna sequence of calretic ... | 2007 | 17449312 |
| molecular cloning, expression of a peroxiredoxin gene in chinese shrimp fenneropenaeus chinensis and the antioxidant activity of its recombinant protein. | peroxiredoxin (prx) is known to be an antioxidant protein that protects the organisms against various oxidative stresses and functions in intracellular signal transduction. a prx gene was firstly isolated in the crustacean, chinese shrimp fenneropenaeus chinensis. the full-length cdna consists of 942bp with a 594bp open reading frame, encoding 198 amino acids. the molecular mass of the deduced amino acid is 22041.17da with an estimated pi of 5.17. sequence comparison showed that prx of f. chinen ... | 2007 | 17481733 |
| cloning and expression of glucose regulated protein 78 (grp78) in fenneropenaeus chinensis. | grp78 (78 kda glucose-regulated protein), also known as bip (immunoglobulin heavy-chain-binding protein), is an essential regulator of endoplasmic reticulum (er) homeostasis because of its multiple functions in protein folding, er calcium binding, and controlling of the activation of transmembrane er stress sensors. in this report, we cloned the full length cdna of grp78 (fcgrp78) from chinese shrimp fenneropenaeus chinensis. this cdna revealed a 2,325 bp with 1,968 bp open reading frame encodin ... | 2009 | 18038268 |
| molecular cloning and characterisation of a pattern recognition protein, lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) from chinese shrimp fenneropenaeus chinensis. | a pattern recognition protein (prp), lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte of chinese shrimp fenneropenaeus chinensis by the techniques of homology cloning and race. analysis of nucleotide sequence revealed that the full-length cdna of 1,275 bp has an open reading frame of 1,098 bp encoding a protein of 366 amino acids including a 17 amino acid signal peptide. sequence comparison of the deduced amino acid sequence of f. chinensis lgbp sh ... | 2009 | 18163220 |
| a novel immortalization vector for the establishment of penaeid shrimp cell lines. | cell immortalization technology based on gene transfer has been successfully used to generate cell lines from a wide variety of cell types. the inability to stably introduce and express foreign genes has hampered application of this strategy in shrimp cells. we report here the use of replication-defective pantropic retrovirus to achieve a novel immortalization vector in which simian virus 40 large t antigen (sv40t) gene is expressed from moloney murine leukemia virus (momlv) promoter. data confi ... | 2008 | 18214628 |
| a toll receptor from chinese shrimp fenneropenaeus chinensis is responsive to vibrio anguillarum infection. | toll-like receptors (tlrs) are an evolutionarily ancient family of pattern recognition receptors (prrs), playing a crucial role in innate immune responses. here we present a toll homolog from chinese shrimp fenneropenaeus chinensis, designated fctoll. the full-length cdna of fctoll is 4115 bp including a poly a-tail of 16 bp, encoding a putative protein of 931 amino acids. the predicted protein consists of an extracellular domain with a potential signal peptide, 16 leucine-rich repeats (lrr), tw ... | 2008 | 18321728 |
| cdna cloning, characterization and expression analysis of the antioxidant enzyme gene, catalase, of chinese shrimp fenneropenaeus chinensis. | catalase is an important antioxidant protein that protects organisms against various oxidative stresses by eliminating hydrogen peroxide. the full-length catalase cdna of chinese shrimp fenneropenaeus chinensis was cloned from the hepatopancreas using degenerate primers by the method of 3' and 5' rapid amplification of cdna ends pcr. the cdna sequence consists of 1892 bp with a 1560 bp open reading frame, encoding 520 amino acids with high identity to invertebrate, vertebrate and even bacterial ... | 2008 | 18353680 |
| synaptonemal complex analysis in spermatocytes of diploid and triploid chinese shrimp fenneropenaeus chinensis. | a modified surface spreading technique for synaptonemal complex (sc) analysis was tested to assess the process of chromosome synapsis in spermatocytes of diploid and induced triploid fenneropenaeus chinensis. spermatocytes of diploid shrimp showed typical morphological characteristics of eukaryote sc, with complete synapsis of bivalents. no recognizable bivalent associated with sex chromosomes was observed in spermatocytes of diploid shrimp. however, differences in morphology of sc, including un ... | 2008 | 18455209 |
| comparison of gene expression profiles of fenneropenaeus chinensis challenged with wssv and vibrio. | microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by wssv and heat-inactivated vibrio anguillarum, respectively. at 6 h post challenge (hpc), a total of 806 clones showed differential expression profile in wssv-challenged samples, but not in vibrio-challenged samples. the genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post wssv-challenged (hpc-wssv) tissues. however, a total of 155 cl ... | 2008 | 18551345 |
| molecular cloning and characterisation of prophenoloxidase (propo) cdna from fenneropenaeus chinensis and its transcription injected by vibrio anguillarum. | the prophenoloxidase(propo) gene was cloned from haemocytes of chinese shrimp fenneropenaeus chinensis by rapid amplification complementary dna ends (race) method. the full-length cdna of prophenoloxidase gene consists of 3040 bp with a 2061 bp open reading frame (orf), encoding 686 amino acids. phylogenetic analysis revealed that it belongs to insect-type invertebrate prophenoloxidase gene family. to understand propo reaction for pathogeny's challenge in shrimp, the expressions of propo in diff ... | 2009 | 18595005 |
| purification and characterization of a natural lectin from the plasma of the shrimp fenneropenaeus chinensis. | a natural lectin from the plasma of the shrimp fenneropenaeus chinensis was purified by singlestep affinity chromatography using fetuin-coupled agarose. the purified plasma lectin showed a strong affinity for human a/b/o erythrocytes (rbc), mouse rbc and chicken rbc. the hemagglutinating (ha) activity of the lectin was dependent on ca(2+) and reversibly sensitive to edta. this lectin was named fc-l and its inactive form had a molecular mass estimate of 168 kda. fifteen n-terminal amino acid sequ ... | 2008 | 18603002 |
| vaccination of shrimp (penaeus chinensis) against white spot syndrome virus (wssv). | two structural protein genes, vp19 and vp466, of white spot syndrome virus (wssv) were cloned and expressed in sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against wssv for shrimps. the cumulative mortalities of the shrimps vaccinated by the injection of rvp19 and rvp466 at 15 days after the challenge with wssv were 50.2% and 51.8%, respectively. for the vaccination by oral feeding of rvp19 and rvp466, the cumulative mortaliti ... | 2008 | 18633299 |
| cloning of cytoplasmic heat shock protein 90 (fchsp90) from fenneropenaeus chinensis and its expression response to heat shock and hypoxia. | heat shock protein 90 (hsp90) works as a multi-functional chaperone and is involved in the regulation of many essential cellular pathways. in this study, we have identified a full-length complementary dna (cdna) of hsp90 (fchsp90) from chinese shrimp fenneropenaeus chinensis. fchsp90 full-length cdna comprised 2,552 bp, including a 2,181-bp open reading frame encoding 726 amino acids. both homology analyses using alignment with previously identified hsp90 and a phylogeny tree indicated that fchs ... | 2008 | 18668349 |
| the manganese superoxide dismutase gene in bay scallop argopecten irradians: cloning, 3d modelling and mrna expression. | a novel manganese superoxide dismutase (mnsod) was cloned from bay scallop argopecten irradians by 3' and 5' rapid amplification of cdna ends (race) pcr. the full-length cdna of mnsod was of 1207 bp with a 678 bp open reading frame encoding 226 amino acids. the deduced amino acid sequence contained a putative signal peptide of 26 amino acids. sequence comparison showed that the mnsod of a. irradians shared high identity with mnsod in invertebrates and vertebrates, such as mnsod from abalone hali ... | 2008 | 18674624 |
| molecular cloning and characterization of proliferating cell nuclear antigen (pcna) from chinese shrimp fenneropenaeus chinensis. | the proliferating cell nuclear antigen gene was cloned from fenneropenaeus chinensis (fcpcna). the full-length cdna sequence of fcpcna encodes 260 amino acids showing high identity with pcnas reported in other species. fcpcna expressed especially high in proliferating tissues of shrimp such as haematopoietic tissue (hpt) and ovary. in order to understand the response of hpt to bacteria and virus challenge, mrna level of fcpcna in hpt was analyzed after shrimp were challenged by vibrio anguillaru ... | 2008 | 18678269 |
| molecular cloning and expression analysis of chymotrypsin-like serine protease from the chinese shrimp, fenneropenaeus chinensis. | a new member of the serine protease (sp) chymotrypsin (designated fc-chy) was isolated from the hepatopancreas of chinese shrimp fenneropenaeus chinensis. the full-length cdna of fc-chy contained 951 nucleotides with a polyadenylation sequence and a polya tail. it encoded a peptide of 271 amino acids with a signal peptide of 17 amino acids and an activation peptide of 28 amino acids. the mature peptide concludes 226 amino acids. it contained the conserved catalytic triad (h, d, and s). similarit ... | 2008 | 18694834 |
| [molecular cloning, recombinant expression and characterization of lysozyme from chinese shrimp fenneropenaeus chinensis]. | lysozyme hydrolyses bacterial cell walls and acts as a nonspecific innate immunity molecule against the invasion of bacterial pathogens. we cloned the cdna of lysozyme from fenneropenaeus chinensis and named fc-lysozyme (fclyz in short). the full length of the gene was of 709 bp, and the open reading frame (477 bp) encoded 158 amino acids. the predicted protein had a signal peptide (-1--18 residue) and molecular weight of the mature protein (residue 1-140) was of 16.2 kd. a lyz 1 domain (residue ... | 2008 | 18724689 |
| a serpin from chinese shrimp fenneropenaeus chinensis is responsive to bacteria and wssv challenge. | arthropod defence responses (e.g. prophenoloxidase (propo) activation and toll pathway initiation) are mediated by serine proteinase cascades and regulated by serpins in haemolymph. a serpin (fc-serpin) cdna was cloned from the haemocytes of fenneropenaeus chinensis by rapid amplification of cdna ends (race) pcr and haemocyte cdna library screening. the full-length cdna consists of 1734bp, encoding 411 amino acids with a calculated molecular mass of 46.55kda and a theoretical isoelectric point o ... | 2009 | 18817877 |
| molecular cloning and characterization of the translationally controlled tumor protein from fenneropenaeus chinensis. | the translationally controlled tumor protein (tctp) is a multi-functioning protein that performs vital roles, particularly in various complicated life processes. in this study, a new tctp cdna was cloned from fenneropenaeus chinensis and hence was designated as fc-tctp. its length is 711 bp, and it is characterized by 507-bp open reading frame that encodes a deduced 168-amino acid protein, including a tctp domain. moreover, this study analyzed the expression patterns of this gene when it respond ... | 2009 | 18853281 |
| molecular cloning of kazal-type proteinase inhibitor of the shrimp fenneropenaeus chinensis. | proteinase inhibitors play important roles in host defence systems involving blood coagulation and pathogen digestion. we isolated and characterized a cdna clone for a kazal-type proteinase inhibitor (kpi) from a hemocyte cdna library of the oriental white shrimp fenneropenaeus chinensis. the kpi gene consists of three exons and two introns. kpi cdna contains an open reading frame of 396 bp, a polyadenylation signal sequence aataaa, and a poly (a) tail. kpi cdna encodes a polypeptide of 131 amin ... | 2009 | 19041263 |
| cdna cloning and gene expression pattern following bacterial challenge of peroxinectin in chinese shrimp fenneropenaeus chinensis. | peroxinectin, a cell-adhesive hemoperoxidase that binds superoxide dismutase and mediates blood cells adhesion and migration in invertebrate, is believed to play an important role in cellular immune reaction. in this study, we reported a new peroxinectin gene homologue from chinese shrimp fenneropenaeus chinensis. based on expressed sequence tags (ests) of haemocyte cdna library, we cloned a 2,611 bps full-length cdna of peroxinectin gene homologue encoded 801 amino acids. motif scanning of the ... | 2009 | 19148772 |
| neutralization of white spot syndrome virus (wssv) for penaeus chinensis by antiserum raised against recombinant vp19. | this study was carried out to neutralize the wssv one of the most virulent pathogen causing large economic damage in shrimp culture industry using the antiserum produced against recombinant wssv envelope protein vp19 (rvp19) as a tool to evaluate wssv infection mechanism. a fragment of vp19 was expressed in sf21 insect cell using baculovirus expression system as fusion protein with 6 his-tag. then, polyclonal antiserum against rvp19 was raised in white rabbit. a constant amount of wssv (at 10(4) ... | 2008 | 19195390 |
| clip domain serine protease and its homolog respond to vibrio challenge in chinese white shrimp, fenneropenaeus chinensis. | clip domain serine proteases and their homologs are involved in invertebrate innate immunity, including hemolymph coagulation, antimicrobial peptide synthesis, cell adhesion, and melanization. recognition of pathogens by pattern recognition receptors can trigger activation of a serine protease cascade. we report here the cdna cloning of a serine protease (fcsp) and a serine protease homolog (fcsph) from chinese white shrimp, fenneropenaeus chinensis. both fcsp and fcsph possess a clip domain at ... | 2009 | 19332134 |
| a selenium-dependent glutathione peroxidase (se-gpx) and two glutathione s-transferases (gsts) from chinese shrimp (fenneropenaeus chinensis). | glutathione s-transferases (gsts) and glutathione peroxidases (gpxs) are essential components of cellular detoxification systems that defend cells against reactive oxygen species (ross). two gstgenes have recently been cloned from fenneropenaeus chinensis and blast p analysis shows that one gst, designated fcmugst, is similar to members of mugst while the other has similarities to thetagst (fcthetagst). a selenium-dependent glutathione peroxidase (se-gpx) has also been cloned from f. chinensis. ... | 2009 | 19358336 |
| trbp homolog interacts with eukaryotic initiation factor 6 (eif6) in fenneropenaeus chinensis. | the hiv transactivating response rna-binding protein (trbp) plays an important role in many biological processes. we have cloned three cdnas from newly identified genes in the trbp family from fenneropenaeus chinensis. these genes have been designated fc-trbp1-3. recombinant fc-trbp1, which was produced in escherichia coli, was used for panning of a t7 phage display library of the chinese shrimp hemocytes. from this panning, fc-eukaryotic initiation factor 6 (fc-eif6) was isolated and sequenced. ... | 2009 | 19380771 |
| expression of four trypsin-like serine proteases from the chinese shrimp, fenneropenaeus chinensis, as regulated by pathogenic infection. | four trypsin-like serine proteases, designated as fctry1, fctry2, fctry3, and fctry4, were obtained from the hepatopancreas of the chinese shrimp, fenneropenaeus chinensis. three trypsin-like serine proteases, specifically fctry1, 2, and 3 were observed to have the conserved catalytic triad (h, d, and s). regarding fctry4, the catalytic triad s was substituted by f, and the mature peptide was found to be inactive. further analysis for similarities indicated that fctry1, 2 and 4 were 89-92% ident ... | 2009 | 19416700 |
| molecular cloning and characterization of the lipopolysaccharide and beta-1, 3-glucan binding protein in chinese mitten crab (eriocheir sinensis). | the lipopolysaccharide and beta-1, 3-glucan binding protein (lgbp) is a pattern recognition protein which is fundamental for the innate immune response of crustaceans. a partial cdna produced by the random sequencing of cdna clones from a haemocyte cdna library of eriocheir sinensis showed similarity to the lgbp gene of the chinese shrimp (fenneropenaeus chinensis). the full-length cdna was subsequently cloned and sequenced by the technique of rapid amplification of cdna ends (race). the e. sine ... | 2009 | 19426826 |
| a novel c-type lectin (fclec4) facilitates the clearance of vibrio anguillarum in vivo in chinese white shrimp. | c-type lectins play important roles in innate immunity of invertebrates. in the present study, we report a novel c-type lectin, named fclec4, from the chinese white shrimp fenneropenaeus chinensis. fclec4 contains a single carbohydrate recognition domain (crd) with a putative signal peptide. phylogenetic analysis indicated that fclec4 was distant from most reported c-type lectins from shrimps. the expression of fclec4 increased at both mrna and protein level after stimulation of vibrio anguillar ... | 2009 | 19447130 |
| identification of a novel inducible cytosolic hsp70 gene in chinese shrimp fenneropenaeus chinensis and comparison of its expression with the cognate hsc70 under different stresses. | the heat shock protein 70 (hsp70) family is widely expressed in eukaryotic cells as the major chaperone protein. in this study, the full-length complementary dna (cdna) of a novel inducible cytosolic hsp70 family member (fchsp70) was cloned from fenneropenaeus chinensis. fchsp70 full-length cdna consists of 2,511 bp with a 1,890-bp open reading frame encoding 629 amino acids. three hsp70 protein family signatures, idlgttys, iidlgggtfdvsil, and ivlvggstripkvqk, were found in the predicted fchsp70 ... | 2010 | 19496024 |
| identification of a novel relish homolog in chinese shrimp fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides. | penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. antimicrobial peptides (amp) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the shrimp amp gene expression is still absent up to date. in the current study, a relish homolog (fcrelish) was cloned from chinese shrimp fenneropenaeus chinensis. the full length cd ... | 2009 | 19520110 |
| a double wap domain (dwd)-containing protein with proteinase inhibitory activity in chinese white shrimp, fenneropenaeus chinensis. | the whey major component, whey acidic protein (wap), has one or more wap domains characterized by a four-disulfide core (4-dsc) structure. these kinds of proteins are involved in multiple functions, including proteinase inhibitor activity, antimicrobial activity, atpase inhibitor activity, and regulatory function in cell proliferation. recent research indicates that wap domain-containing proteins play an important role in the innate immunity of crustaceans. in this study, a novel double wap doma ... | 2009 | 19545640 |
| identification and molecular characterization of a spätzle-like protein from chinese shrimp (fenneropenaeus chinensis). | in invertebrates, the toll signaling pathway is important for activation of antimicrobial peptides in the innate immune system. activation of the toll pathway requires binding of toll with its ligand spätzle. here we described a spätzle-like protein, designated as fc-spz, from hemocytes of chinese shrimp, fenneropenaeus chinensis. the deduced amino acid sequence of fc-spz shares 54% identity with spätzle-like protein of salmon louse (lepeophtheirus salmonis). semi-quantitative reverse transcript ... | 2009 | 19616633 |
| cloning of profilin (fcpfn) from the shrimp fenneropenaeus chinensis, a highly expressed protein in white spot syndrome virus (wssv)-infected shrimp. | we isolated and characterized the profilin (fcpfn) cdna from hemocytes of fenneropenaeus chinensis, a unique shrimp species from the yellow sea. the fcpfn cdna consists of 830 bp and encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.06. the deduced amino acid sequence of fcpfn shows 36% and 90% amino acid sequence identity to the profilin genes of pacific white shrimp litopenaeus vannamei and black tiger shrimp penaeus monodon, respectively. the fcpfn mrna was h ... | 2009 | 19638679 |
| molecular characterization and expression analysis of chitinase (fcchi-3) from chinese shrimp, fenneropenaeus chinensis. | complementary dna (cdna) and genomic dna, including flanking regions of the chitinase gene (fcchi-3) of fenneropenaeus chinensis, were cloned and sequenced. fcchi-3 was found to have 92.0 and 91.4% identity at the cdna level to that of litopenaeus vannamei and marsupenaeus japonicus, respectively. the predicted amino acid sequence had an overall similarity with a comparable region of l. vannamei (96.8%) and m. japonicus (93.4%). based on the cdna sequence, the genomic structure of the gene was c ... | 2010 | 19642017 |
| a c-type lectin is involved in the innate immune response of chinese white shrimp. | c-type lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this work, a cdna for a new c-type lectin, fclec3, was obtained from chinese white shrimp fenneropenaeus chinensis using expressed sequence tag analysis and rapid amplification of the cdna ends. fclec3 contains an n-terminal signal peptide and a carbohydrate recognition domain (crd). rt-pcr analysis showed that fclec3 was mainly expressed in hepatopancreas and that the expression ... | 2009 | 19647083 |
| a thioredoxin response to the wssv challenge on the chinese white shrimp, fenneropenaeus chinensis. | thioredoxin (trx) is involved in cell redox homeostasis. in addition, it is responsible for maintaining proteins in their reduced state. in our study, a fenneropenaeus chinensis thioredoxin (fctrx) gene was identified from the chinese white shrimp. the full length of fctrx was 777 bp, including a 60 bp 5' untranslated region (utr), a 318 bp open reading frame (orf) encoding a 105 amino acids protein, and a 399 bp 3' utr. fctrx contained a trx domain with a conserved motif of cys-gly-pro-cys (cgp ... | 2010 | 19747568 |
| an acyl-coa-binding protein (fcacbp) and a fatty acid binding protein (fcfabp) respond to microbial infection in chinese white shrimp, fenneropenaeus chinensis. | acyl-coa-binding protein (acbp) and fatty acid-binding protein (fabp) are involved in lipid metabolism. acbp plays a key role in multiple cellular tasks including modulation of fatty acid biosynthesis, enzyme regulation, vesicular trafficking, and gene regulation. in our study, a 536 bp cdna of acbp (fcacbp) was cloned and identified as a widely distributed gene in the chinese white shrimp, fenneropenaeus chinensis. its expression in intestine was upregulated in response to white spot syndrome v ... | 2009 | 19766195 |
| immune responses of fenneropenaeus chinensis against white spot syndrome virus after oral delivery of vp28 using bacillus subtilis as vehicles. | the protective efficacy of oral administration of vp28 using bacillus subtilis as vehicles (rvp28-bs) in shrimp, fenneropenaeus chinensis, upon challenge with white spot syndrome virus (wssv) was investigated. the calculated relative percent survival (rps) value of rvp28-bs fed shrimp was 83.3% when challenged on the 14th day post-administration, which is significantly higher (p < 0.001) than that of the group administered recombinant escherichia coli over-expressing rvp28 (rvp28-e21). after imm ... | 2010 | 19800009 |
| three tetraspanins from chinese shrimp, fenneropenaeus chinensis, may play important roles in wssv infection. | three members of the tetraspanin/tm(4)sf superfamily were cloned from chinese shrimp, fenneropenaeus chinensis. the deduced amino acid sequences of the three proteins have typical motifs of the tetraspanin/tm(4)sf superfamily. phylogenetic analysis of the proteins, together with the known tetraspanins of invertebrates and vertebrates, revealed that they belong to different tetraspanin subfamilies: cd9, cd63 and tetraspanin-3. the three cloned genes of cd9, cd63 and tetraspanin-3 showed apparentl ... | 2010 | 19943839 |
| vibrio atypicus sp. nov., isolated from the digestive tract of the chinese prawn (penaeus chinensis o'sbeck). | a culture (designated strain hhs02(t)) was isolated from chinese prawn (penaeus chinensis, o'sbeck) and determined to be a member of the genus vibrio. strain hhs02(t) comprised slightly curved, rod-shaped, non-endospore-forming, gram-negative, catalase-negative, oxidase-positive, o/129-sensitive and facultatively anaerobic cells that were motile by means of a single polar flagellum. growth of strain hhs02(t) occurred in 0.5-7 % (w/v) nacl [optimally in 1-3 % (w/v) nacl] and between ph 7.0 and 10 ... | 2010 | 19965995 |
| molecular cloning and characterization of three crustins from the chinese white shrimp, fenneropenaeus chinensis. | antibacterial peptides crustins are the effector molecules of innate immunity in decapods. in this study, three crustin cdna sequences (fc-crus 1, fc-crus 2, and fc-crus 3) were cloned from the chinese white shrimp fenneropenaeus chinensis. the full-length cdnas of fc-crus 2 and 3 are 473 bp and 574 bp, respectively. the deduced peptides of fc-crus 2 and 3 contain a signal peptide and a crustin domain at the c-terminal formed by twelve conserved cysteine residues. the partial sequence of fc-cru ... | 2010 | 20036745 |
| multiple forms of alpha-2 macroglobulin in shrimp fenneropenaeus chinesis and their transcriptional response to wssv or vibrio pathogen infection. | alpha-2 macroglobulin (a2m) is a non-specific protease inhibitor involved in host defense. by full length cloning and sequencing we identified three distinct cdnas for a2m in chinese shrimp fenneropenaeus chinesis, designated fca2m-1, fca2m-2 and fca2m-3, respectively. expression profiles in normal tissues as well as tissues after challenge by white spot syndrome virus (wssv) and vibrio pathogen were conducted for fca2m-1 and fca2m-2. the fca2m-1 and fca2m-2 cdnas encode proteins with 1501 or 15 ... | 2010 | 20105438 |
| a new c-type lectin (fclec5) from the chinese white shrimp fenneropenaeus chinensis. | c-type lectins are one family of pattern recognition receptors (prrs) that play important roles in innate immunity. in this work, cdna and genomic sequences for a new c-type lectin (fclec5) were obtained from the chinese white shrimp fenneropenaeus chinensis. fclec5 cdna contains an open reading frame of 1,008 bp and its genomic sequence is 1,137 bp with 4 exons and 3 introns. the predicted fclec5 protein contains a signal peptide and two carbohydrate recognition domains (crds). the n-terminal c ... | 2010 | 20349323 |
| two cysteine proteinases respond to bacterial and wssv challenge in chinese white shrimp fenneropenaeus chinensis. | the cdnas encoding cathl and legumain from chinese white shrimp fenneropenaeus chinensis (fccathl, fclegu) were obtained. both fccathl and fclegu mrna were expressed mainly in the hepatopancreas of unchallenged shrimp. time-course analysis of fccathl showed that fccathl was upregulated in the hepatopancreas of shrimp challenged with white spot syndrome virus (wssv) at 12 h. fclegu mrna in hepatopancreas was down-regulated by vibrio. fclegu transcript first declined from 2 h to 6 h and then recov ... | 2010 | 20362060 |
| a dorsal homolog (fcdorsal) in the chinese shrimp fenneropenaeus chinensis is responsive to both bacteria and wssv challenge. | rel/nfkappab is a family of transcription factors. in the present study, a rel/nfkappab family member, dorsal homolog (fcdorsal) was cloned from the chinese shrimp fenneropenaeus chinensis. the full length cdna of fcdorsal consists of 1627bp, revealed a 1071bp open reading frame encoding 357 aa. the predicted molecular weight (mw) of the deduced amino acid sequence of fcdorsal was 39.78kda, and its theoretical pi was 8.85. amino acid sequence analysis showed that fcdorsal contains a rel homolog ... | 2010 | 20363249 |
| a real-time pcr for the detection of hepatopancreatic parvovirus (hpv) of penaeid shrimp. | hepatopancreatic parvovirus (hpv) causes a common shrimp disease that occurs in many shrimp farming regions, especially in the indo pacific, and infects most of the cultured penaeid species. there are seven geographic hpv isolates known, so a method to detect different hpv types is needed. we developed a sensitive and generic real-time pcr assay for the detection of hpv. a pair of primers and taqman probe based on an hpv sequence obtained from samples of fenneropenaeus chinensis from korea were ... | 2010 | 20367743 |
| proteomic analysis of differentially expressed proteins in lymphoid organ of fenneropenaeus chinensis response to vibrio anguillarum stimulation. | to gain an insight into the function of shrimp lymphoid organ at protein level, we analyzed the proteome of lymphoid organ in healthy chinese shrimp fenneropenaeus chinensis (f. chinensis) through two-dimensional gel electrophoresis (2-de) based proteomic approach. a total of 95 spots representing 75 protein entries were identified by liquid chromatography tandem mass spectrometry (lc-ms/ms) with both online and in-house database. according to gene ontology (go) annotation of biological process, ... | 2010 | 20394824 |
| screening of genes related to ovary development in chinese shrimp fenneropenaeus chinensis by suppression subtractive hybridization. | the ovary of triploid shrimp fenneropenaeus chinensis was apparently impaired compared to that of the diploid shrimp at the same age. therefore triploid shrimp ovary is possible to be taken as a model to understand the mechanism of ovary development of shrimp compared to that of the ovary of diploid shrimp at the same age. in the present study, a suppression subtractive hybridization (ssh) technique was applied to identify differentially expressed genes in the ovary between diploid and triploid ... | 2010 | 20403774 |
| molecular cloning of daphnia magna catalase and its biomarker potential against oxidative stresses. | catalase (ec 1.11.1.6) is an important antioxidant enzyme that protects aerobic organisms against oxidative damage by degrading hydrogen peroxide to oxygen and water. catalase mrnas have been cloned from many species and employed as useful biomarkers of oxidative stress. in the present study, we cloned the cdna from the catalase gene in daphnia magna, analyzed its catalytic properties, and investigated mrna expression patterns after the exposure to known oxidative stressors. the catalase proxima ... | 2010 | 20470898 |
| comparative proteomic profiles of the hepatopancreas in fenneropenaeus chinensis response to white spot syndrome virus. | white spot syndrome virus (wssv) infects all shrimp species and is the greatest detriment to shrimp culture. to better understand the mechanism of molecular responses to wssv infection in chinese white shrimp fenneropenaeus chinensis, two dimensional electrophoresis (2-de) was used. differentially expressed proteins in the hepatopancreas of control and wssv-injected chinese white shrimp between (6, 12 and 24 h post-injection) were screened. quantitative intensity analysis and mass spectrometry r ... | 2010 | 20580833 |
| predicted sub-populations in a marine shrimp proteome as revealed by combined est and cdna data from multiple penaeus species. | abstract: | 2010 | 21067619 |
| identification of three different types of serine proteases (one sp and two sphs) in chinese white shrimp. | serine proteases (sps) and serine protease homologs (sphs) participate in digestion, embryonic development, blood coagulation, and immune defense responses. in this paper, we identify one sp and two sphs, including a masquerade sph (fcmas), a cub domain containing sp (fccubsp), and a single domain containing sph (fcsph2) in chinese white shrimp, fenneropenaeus chinensis. fcmas has a gly-rich region formed by three repeats of lggqggg, a clip domain and a c-terminal sp-like domain. absence of ser ... | 2010 | 21109005 |
| molecular cloning and expression pattern analysis of two novel disulfide isomerases in shrimp. | protein disulfide isomerase (pdi) catalyzes formation and isomerization of disulfide bridges and has chaperone activity. currently, increasing evidence suggests the significance of pdi in immune and stress responses. to clarify the role of pdis in the innate immunity of shrimp, two pdi genes were isolated and identified from fenneropenaeus chinensis (fleshy prawn). fcpdi1 is 1878bp in length and encodes a protein of 383 amino acids. it has 18-amino acid signal peptide, 3 thioredoxin domains with ... | 2010 | 21130898 |
| the possible role of penaeidin5 from the black tiger shrimp, penaeus monodon, in protection against viral infection. | penaeidin class 5 (pen5) has so far only been reported in the chinese shrimp, fenneropenaeus chinensis, and the black tiger shrimp, penaeus monodon. the pen5 homolog from f. chinensis (fenchipen5) exhibits antimicrobial activities against both gram-positive and gram-negative bacteria as well as fungi. here, we characterized the pen5 gene from p. monodon (penmonpen5) and evaluated its potential involvement in antiviral immunity. the deduced open reading frame of penmonpen5 encodes for a predicted ... | 2010 | 21199664 |
| molecular cloning and characterization of a receptor for activated protein kinase c1 (rack1) from chinese white shrimp; fenneropenaeus chinensis. | receptor for activated c kinase 1 (rack1), which has seven tandem wd40 domains, is a scaffolding protein. rack1 plays different roles by binding to different partner proteins. it is involved in hormone signaling and development, and now some evidence indicates it may have a role in innate immunity. in this paper, rack1 cdna from chinese white shrimp (fcrack1) was identified. the full length of the fcrack1 gene is 1037 bp, including a 30 bp 5'utr, a 957 bp orf encoding a 318 amino acid protein, a ... | 2011 | 21238484 |
| molecular cloning and expression analysis of signal transducer and activator of transcription (stat) from the chinese white shrimp fenneropenaeus chinensis. | innate immunity is the first line of defense by a host against invading pathogens. several signaling pathways participate in the immune response, one of which is the janus kinase (jak)/signal transducer and activator of transcription (stat) pathway. various evidences have been provided to suggest that the jak/stat pathway is involved in both antibacterial and antiviral immunities. in this study, the full-length cdna and gene sequence of stat (designated as fcstat) was cloned from the chinese whi ... | 2011 | 21246285 |
| involvement of peroxinectin in the defence of red swamp crayfish procambarus clarkii against pathogenic aeromonas hydrophila. | cell adhesion factors are important immune components for invertebrate to immobilize, phagocytose or encapsulate invasive microorganisms and foreign particles. in this study, a new cell adhesion factor, peroxinectin (refered as pcpxin) was isolated from hemocytes of red swamp crayfish (procambarus clarkii). the full-length cdna of pcpxin was 3014 bp encoding a protein of 819 amino acid residues with a predicted molecular weight of 89.0 kda and a calculational isoelectric point of 6.93. the putat ... | 2011 | 21539922 |
| enzyme e2 from chinese white shrimp inhibits replication of white spot syndrome virus and ubiquitinates its ring domain proteins. | recent studies have shown that the ubiquitin (ub) proteasome pathway (upp) is closely related to immune defense. we have identified a ubiquitin-conjugating enzyme, e2, from the chinese white shrimp, fenneropenaeus chinensis (fcubc). injection of recombinant fcubc protein (rfcubc) reduced the mortality of shrimp infected with white spot syndrome virus (wssv) and inhibited replication of wssv. rfcubc, but not a mutant fcubc (mfcubc), bound to wssv ring domains (wrds) from four potential e3 ligase ... | 2011 | 21680526 |
| cloning and characterization of rap gtpase from the chinese white shrimp fenneropenaeus chinensis. | ras-related protein rap gtpase has been implicated in cell adhesion, cell proliferation, and cell junction formation. the first shrimp rap cdna (fcrap) was recently identified from the chinese white shrimp fenneropenaeus chinensis. the full length of fcrap is 1013bp, with a 561bp open reading frame that encodes a 186 amino acid protein. fcrap has a calculated molecular mass of 20.90kda and pi of 6.37. phylogenetic analysis shows that fcrap and other rap proteins are clustered into one group. res ... | 2011 | 21801745 |
| A novel pathogen-binding gC1qR homolog, FcgC1qR, in the Chinese white shrimp, Fenneropenaeus chinensis. | In vertebrates, the globular "head" of complement component C1q receptor (gC1qR) is a versatile, multiligand binding protein. However, research on its function in invertebrates is limited. In the present study, a full-length cDNA sequence of a novel gC1qR homolog, FcgC1qR, from the Chinese white shrimp Fenneropenaeus chinensis was cloned. Semi-quantitative polymerase chain reaction (PCR) detected FcgC1qR in all examined tissues, with the highest level detected in the intestine. Western blot assa ... | 2012 | 21893092 |
| a homolog of the cell apoptosis susceptibility gene involved in ovary development of chinese shrimp fenneropenaeus chinensis. | the cell apoptosis susceptibility (cas) gene is a homolog of the yeast chromosome segregation (cse1) gene, which functions in cell proliferation and apoptosis. in the present study, a homolog of cas was cloned from chinese shrimp fenneropenaeus chinensis (fccas). the full-length fccas cdna is 3534 bp and contains an open reading frame encoding 968 amino acids. the predicted tertiary fccas structure is highly similar to that of cse1 from the yeast saccharomyces cerevisiae. rt-pcr analysis showed ... | 2011 | 21900679 |
| complete nucleotide sequence analysis of a korean strain of hepatopancreatic parvovirus (hpv) from fenneropenaeus chinensis. | hepatopancreatic parvovirus (hpv) of shrimp is distributed worldwide and the entire genome of thailand and indian strains (pmdnv) and one australian strain (pmergdnv) have now been reported. the complete nucleotide sequence of a hpv strain isolated from the fleshy prawn fenneropenaeus chinensis in korea (fcdnv) was determined and compared to previously reported sequences. the entire genome of fcdnv contains 6,336 nucleotides, with 40% g+c content, which is the biggest of the known hpv strains. t ... | 2011 | 21948006 |
| molecular cloning, expression analysis and characterization of peroxiredoxin during wssv infection in shrimp fenneropenaeus indicus. | innate immunity is the first line of defense in shrimps against invading pathogenic microorganisms. peroxiredoxins (prx) are the family of antioxidant proteins that play a crucial role in reduction of oxidative stress in host during viral infection. peroxiredoxin from fenneropenaeus indicus was identified, cloned and expressed in prokaryotic expression system. the fi-prx protein consists of two conserved cys residues and belongs to typical 2-cys prx family. the phylogenetic analysis revealed a c ... | 2012 | 21964313 |
| A TaqMan Real-Time PCR Assay for Quantifying Type III Hepatopancreatic Parvovirus Infections in Wild Broodstocks and Hatchery-Reared Postlarvae of Fenneropenaeus chinensis in Korea. | A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatcheryreared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders w ... | 2011 | 22127120 |
| trbp and eif6 homologue in marsupenaeus japonicus play crucial roles in antiviral response. | plants and invertebrates can suppress viral infection through rna silencing, mediated by rna-induced silencing complex (risc). trans-activation response rna-binding protein (trbp), consisting of three double-stranded rna-binding domains, is a component of the risc. in our previous paper, a trbp homologue in fenneropenaeus chinensis (fc-trbp) was reported to directly bind to eukaryotic initiation factor 6 (fc-eif6). in this study, we further characterized the function of trbp and the involvement ... | 2012 | 22279564 |
| blocking the large extracellular loop (lel) domain of fctetraspanin-3 could inhibit the infection of white spot syndrome virus (wssv) in chinese shrimp, fenneropenaeus chinensis. | tetraspanins belong to the transmembrane 4 superfamily (tm(4)sf), which span the cell membrane 4 times and act as bridges or connectors. increasing evidences have shown that tetraspanins play important role in virus infection. the large extracellular loop (lel) of a tetraspanin is considered as a possible target of some virus. tetraspanins are widely found in invertebrates, but the functional roles of most invertebrate tetraspanins have remained unknown. recently, a tetraspanin, called fctetrasp ... | 2012 | 22406449 |
| identification and characterization of the sex-determiner transformer-2 homologue in chinese shrimp, fenneropenaeus chinensis. | the transformer-2 gene, encoding a protein (tra-2) which directs sex-specifically alternative splicing of doublesex (dsx) pre-mrna in combination with the transformer (tra) protein, has been proved to play important roles on sex differentiation and sex development in drosophila melanogaster. in the present study, a tra-2 homologue (fctra-2) was cloned and characterized in the chinese shrimp, fenneropenaeus chinensis. a fctra-2 genomic dna sequence with a length of 8,871 bp was obtained and verif ... | 2012 | 22846336 |
| involvement of fenneropenaeus chinensis cathepsin c in antiviral immunity. | cathepsin c (cath c) is a lysosomal cysteine protease that belongs to the papain superfamily. cath c is capable of activating many chymotrypsin-like serine proteases and is reported to be a central coordinator for the activation of many serine proteinases in immune and inflammatory cells. in this study, cath c cdna was cloned from fenneropenaeus chinensis (fc). the complete cdna of fc-cath c in chinese white shrimp was found to be 1445-base pairs (bp) long. it contained an open reading frame (or ... | 2012 | 22885030 |
| the vp37-binding protein f1atp synthase β subunit involved in wssv infection in shrimp litopenaeus vannamei. | to investigate the interaction between white spot syndrome virus (wssv)-vp37 and gill membrane proteins (gmps) of pacific white shrimp (litopenaeus vannamei), the vp37 protein was expressed and purified, and a distinct 53 kda vp37-binding protein band was identified in gmps by virus overlay protein binding assay and gst pull-down assay. by electroelution, the vp37 binding protein was purified and identified as f(1)atp synthase β (f(1)atpase β) subunit by mass spectrometry. the purified f(1)atpas ... | 2013 | 23108256 |
| role of chymotrypsin-like serine proteinase in white spot syndrome virus infection in fenneropenaeus chinensis. | white spot syndrome virus (wssv) caused a great economic loss in shrimp aquaculture. although great efforts have been undertaken to characterize the virus disease during the last two decades, there are still lack of effective methods to prevent or cure it. in this study, we investigated the transcriptional expression profiles of 18 key immune-related genes in the chinese shrimp fenneropenaeus chinensis which was severely infected by wssv. we found that the expression levels of 6 genes including ... | 2013 | 23142726 |
| identification and molecular characterization of a c-type lectin-like protein from chinese shrimp (fenneropenaeus chinensis). | a c-type lectin-like protein was cloned and characterized from the chinese shrimp fenneropenaeus chinensis, and named as fcctl. the results indicated that the full length cdna of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved cys and one key motif epgd. the theoretical molecular weight and pi of mature protein was 25.3 kda and 5.4. sequence comparison of the deduced amino acid sequence of fcctl showed varied ident ... | 2013 | 23192616 |
| shrimp myd88 responsive to bacteria and white spot syndrome virus. | the myeloid differentiation factor 88 (myd88) is an important adapter protein which links members of the toll-like receptor (tlr) to the downstream components to activate related signaling pathways. in the present study, a myd88 homolog (fcmyd88) was cloned from penaeid shrimp fenneropenaeus chinensis. the orf of fcmyd88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (dd) and a typical tlr and interleukin-1 receptor (il-1r)-related (tir) domain. homo ... | 2013 | 23238389 |
| an iκb homologue (fccactus) in chinese shrimp fenneropenaeus chinensis. | this study firstly reports the characterization of a functional iκb homologue, fccactus in chinese shrimp fenneropenaeus chinensis. the full length cdna of fccactus consists of a 1359 bp open reading frame (orf) encoding a 453 amino acid protein with a predicted molecular weight (mw) of 48.46 kda and theoretical pi of 5.23. phylogenetic analysis and multiple alignments revealed that the deduced amino acid sequence of fccactus cdna had high similarities to cactus or iκb reported in seven other ar ... | 2012 | 23276882 |
| genomic organization and promoter characterization of fcctl, a c-type lectin-like protein in fenneropenaeus chinensis. | diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. c-type lectins may play an important role in shrimp immunity. the gene encoding fcctl, a c-type lectin-like protein, was isolated from fenneropenaeus chinensis and characterized. this gene was composed of five exons spanning 2470 base pairs (bp). intron 1 contained a possible promoter and many potential transcription factor binding sites, such as those for tata binding protein, creb, srf, ttk and three sites f ... | 2013 | 23337594 |
| transcriptome analysis on chinese shrimp fenneropenaeus chinensis during wssv acute infection. | previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (wssv) infection in crustacean. however, little information is available in relation to underlying mechanisms of host responses during the wssv acute infection stage in naturally infected shrimp. in this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. a tot ... | 2013 | 23527000 |
| identification and characterization of a serine protease inhibitor esserpin from the chinese mitten crab eriocheir sinensis. | serine protease inhibitors (serpins) represent an expanding superfamily of endogenous inhibitors that regulate proteolytic events and involve in a variety of physiological processes. a serine protease inhibitor, namely esserpin, was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) analysis. the full-length cdna of esserpin was of 2367 bp, including an open reading frame (orf) of 1371 bp encoding a polypeptide of 456 amino acids with estimated molecular ... | 2013 | 23567854 |