Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| defect in translation of poliovirus rna at the restrictive temperature. | 1976 | 184290 | |
| physical and metabolic requirements for early interaction of poliovirus and human rhinovirus with hela cells. | attachment, ""tight binding'' and eclipse of radioactive poliovirus 2 (p2) and human rhinovirus 2 (hrv 2) were investigated. the activation energy for attachment of both hrv2 and p2 was about 13 kcal/mol. hrv2 differed from p2 in two respects: the arrhenius plot for attachment of hrv2 showed a break at 15 to 19 degrees c when the cells were first treated several hours at 0 degrees c, and attachment of hrv2 was inhibited by treatment of cells with metabolic poisons able to reduce cellular atp by ... | 1976 | 184301 |
| inactivation of poliovirus in water by ozonation. | 1976 | 184306 | |
| "fingerprinting" high molecular weight rna by two-dimensional gel electrophoresis: application to poliovirus rna. | conditions are described under which complete rnase t1 digests of high molecular weight rna can be separated into numerous components by two-dimensional gel electrophoresis. small and large oligonucleotides (n = 1 - 2c0) can be resolved without losses. the procedure yields fingerprints which are diagnostic for a particular species of rna and an index of its purity as will be shown for the genomes of poliovirus type 1 and 2. | 1976 | 184435 |
| interferon-induced inhibition of protein synthesis in l-cell extracts: an atp-dependent step in the activation of an inhibitor by double-stranded rna. | the translation of encephalomyocarditis virion rna in extracts from interferon-treated l-cells is inhibited by the addition of double-stranded rna (dsrna) at 400 ng/ml. a similar inhibition in response to dsrna is seen in control cell extracts supplemented with small amounts of a postribosomal supernatant fraction from interferon-treated cells (interferon cell sap): neither interferon cell sap nor dsrna alone is inhibitory in control systems. the inhibition is much reduced if translation is carr ... | 1976 | 184463 |
| seroimmunity of adult ghanian hospital staff and their wards to poliomyelitis. | the antibody content of sera collected from 104 adult staff of the korle bu teaching hospital and 16 of their wards were studied by microtitre neutralization test to poliovirus types 1, 2 and 3. the immune status of the study population was highly satisfactory; there was only one (0.83%) out of the 120 persons studied without antibodies to all three poliovirus types. the percentage of sero-positive subjects to the individual poliovirus types, that is 1, 2 and 3 were 75.83%, 70.00% and 86.16% of ... | 1975 | 184602 |
| stability of human enteroviruses in estuarine and marine waters. | studies of the effects of temperature and salinity on the survival of three enteric viruses (poliomyelitis type 1, echovirus-6, and coxsackievirus b-5) under controlled laboratory conditions and in situ indicate that temperature rather than salinity is the critical factor affecting their stability, in that the higher the temperature the more rapid was the loss of viral infectivity. in the laboratory studies, all three viruses were quite stable at 4 degrees c, with infectious virus still detectab ... | 1976 | 184736 |
| a complex between poliovirus rna and the structural polypeptide vp1. | 1976 | 184788 | |
| comparison of four eluents in the recovery of indigenous viruses from raw sludge. | the efficiency of 3% casein hydrolysate (ch), 3% lactalbumin hydrolysate (lh), 3% beef extract (be), and 10% fetal calf serum (fcs) was compared for the recovery of viruses from raw sludge. ch and lh proved to be inefficient and were eliminated from the study after initial testing. in tests with 20 different samples of raw sludge, beef extract eluted virus in 15 (75%) and fcs revealed virus in 19 (95%) of the samples using bs-c-1 cells. that different eluents were not eluting different viruses f ... | 1976 | 184901 |
| polyadenylate sequences of human rhinovirus and poliovirus rna and cordycepin sensitivity of virus replication. | the polyadenylate [poly(a)] content of the genome rna of human rhinovirus type 14 (hrv-14) is nearly twice as large as that of the genome rna of poliovirus type 2. the poly(a) content of viral rna was determined to be the rnase-resistant fraction of 32p-labeled viral rna extracted from purified virions. polyacrylamide gel electrophoresis indicated that the poly(a) sequences of hrv-14 are more heterogenous and on an average larger than those of poliovirus rna. on the basis of susceptibility to mi ... | 1976 | 185411 |
| [is the rheumatoid arthritis of children a late complication of rubella? (proceedings)]. | 1976 | 185840 | |
| [studies on virus inactivation by chlorine during water disinfection (author's transl)]. | the studies described indicate a potential for water-borne transmission of viral diseases and the problems involved in virus inactivation by means of water chlorination. in contrast to the amount of free chlorine, the value of the oxidation-reduction potential (orp) was found to be a criterion of virus inactivation. for virus inactivation, higher orp values and longer periods of contact than for the killing of bacteria, respectively, were found to be necessary. to ensure the inactivation of poli ... | 1976 | 185851 |
| specific inactivation of herpes simplex virus by silver nitrate at low concentrations and biological activities of the inactivated virus. | the infectivities of herpes simplex virus types 1 and 2 were inactivated by silver nitrate at concentrations of 30 mum or less, which did not affect at all the infectivities of hemagglutinating virus of japan, vesicular stomatitis virus, poliovirus, vaccinia virus, and adenovirus. the inactivated virus retained the capability of adsorbing to the cell, with an adsorption kinetics quite similar to that of intact virus, and of inducing the concanavalin a agglutinability in the infected cells, where ... | 1976 | 185946 |
| heat inactivation of poliovirus in wastewater sludge. | the effect of raw and anaerobically digested sludge on heat inactivation of poliovirus was investigated. raw sludge was found to be very protective of poliovirus plaque-forming ability at all temperatures studied, but digested sludge had variable effects that were highly dependent upon the experimental conditions. in low concentrations and at relatively low inactivation temperatures, digested sludge is nearly as protective of poliovirus as raw sludge. however, at higher tempeatures and concentra ... | 1976 | 185956 |
| virus movement in soil columns flooded with secondary sewage effluent. | secondary sewage effluent containing about 3 x 10(4) plaque-forming units of polio virus type 1 (lsc) per ml was passed through columns 250 cm in length packed with calcareous sand from an area in the salt river bed used for ground-water recharge of secondary sewage effluent. viruses were not detected in 1-ml samples extracted from the columns below the 160-cm level. however, viruses were detected in 5 of 43 100-ml samples of the column drainage water. most of the viruses were adsorbed in the to ... | 1976 | 185960 |
| the morphology of tumors of the human gastrointestinal tract in short-term organ culture and the reaction of these tumors to infection with poliovirus. | a morphologic study of 57 cancers of the human gastrointestinal tract maintained in short-term organ culture was carried out, and the response of part of these tumors to infection with poliovirus was studied. the tumors, grown in vitro, mainly retained their original characteristics and histologic types. a certain correlation between the histologic type and its viability in short-term organ culture was revealed: the more differentiated forms were maintained better than the poorly differentiated ... | 1976 | 186175 |
| potency of field samples of oral poliovirus vaccine. | the transport and storage facilities and the potency of oral poliovirus vaccines currently administered in 108 centres in india were investigated. storage and distribution practices in many of the centres were far from ideal. there was no significant loss of potency in the vaccine samples collected from a few centres, while samples from other centres showed a 60-99% loss of virus particles per dose. a national monitoring system has since been established to check the potency of every batch of or ... | 1976 | 186207 |
| some characteristics of poliovirus strains isolated in uganda between 1966 and 1971. | sixty-five poliovirus strains were investigated in genetic marker tests in order to obtain information on the characteristics of polioviruses circulating in uganda where, owing to the insufficient use of live poliovirus vaccine, poliomyelitis remained a serious public health problem. of the type 1 strains predominant in both epidemic and non-epidemic years, 29 were studied for their antigenic fine structure. based on their intratypic character, these strains proved to represent six different ant ... | 1976 | 186208 |
| effect of hypertonic medium on protein synthesis of mahoney and lsc poliovirus. | the initiation of synthesis of mahoney virus protein is more sensitive to high osmolarity than initiation of synthesis of lsc virus protein. production of mahoney virus protein appears to be only slightly more resistant to high osmolarity than synthesis of hela-rhino cell protein. | 1976 | 186637 |
| concentration of viruses from large volumes of tap water using pleated membrane filters. | a method is described for the efficient concentration of viruses from large volumes of tap water in relatively short time periods. virus in acidified tap water in the presence of aluminum chloride is adsorbed to a 10-inch (ca. 25.4 cm) fiberglass depth cartridge and a 10-inch pleated epoxy-fiberglass filter in series at flow rates of up to 37.8 liters/min (10 gallons/min). this filter series is capable of efficiently adsorbing virus from greater than 19,000 liters (5,000 gallons) of treated tap ... | 1976 | 187115 |
| detection of virus in water: sensitivity of the tentative standard method for drinking water. | the sensitivity of several microporous virus-adsorbent media for reliably detecting low levels of poliovirus from 380 and 1,900 liters of drinking water by use of the tentative standard method was investigated. the virus-adsorbent media tested were (i) nitrocellulose membrane filters, (ii) epoxy-fiber glass-asbestos filters, (iii) yarn-wound fiber glass depth filters, and (iv) epoxy-fiber glass filter tubes. virus was adsorbed to the filter media at ph 3.5 and eluted with glycine buffer, ph 11.5 ... | 1976 | 187116 |
| a perfusion culture system for virus vaccine manufacture in diploid cell cultures. | development of a new perfusion culture system for the production of attenuated poliomyelitis virus in cultures of diploid cells is described. the growth characteristics of the diploid cells (mrc-5) were found to be normal in the perfused system. procedures for the routine production of cell cultures at twice the cell density of stationary bottle cultures were established. the yield of virus (lsc 2ab) per cell and per unit of surface growth area were observed to be significantly higher in the per ... | 1976 | 187274 |
| immune response to acute otitis media in children iii. implications of viral antibody in middle ear fluid. | with the technique of radioimmunodiffusion and indirect fa staining, iga antibody to measles, mumps, rubella, and polio-1 were determined in serum and middle ear fluid (mef) of 103 patients with otitis media. the occurrence of iga viral antibody in mef and its absence in simultaneously drawn serum was used as an indicator of local antibody production. of the 401 assays performed, 41 instances of iga antibody exclusively in mef were found. only four of these occurred in specimens from unimmunized ... | 1977 | 187700 |
| amino acid composition of the poliovirus capsid polypeptides isolated as fluorescamine conjugates. | poliovirus was treated with fluorescamine and disrupted with sodium dodecyl sulphate (sds). the polypeptides were isolated by electrophoresis in a polyacrylamide gel (sds-page) and their amino acid compositions determined. the compositions of vp1, 2 and 3 were very similar but different from that of vp4. | 1976 | 187727 |
| provocation poliomyelitis and entry of poliovirus to the cns. | it is proposed that entry to the cns by poliovirus occurs from multiple sites which are seeded during viremia. at each site, an inflammatory reaction allows entry to peripheral nerves. differences in incubation times are related to the length of nerve to be followed. virus reaches the cns sequentially but does not in natural poliomyelitis spread within the cns. provoking agents are identified as immunogenic and their action explained. details of paralysis among cutter vaccinees are explained by ... | 1976 | 187911 |
| [the concept of risk in poliomyelitis (author's transl)]. | in poliomyelitis the risk is of neurological accidents resulting either from the circulation of wild viruses (spontaneous risk) or from the introduction of virus-vaccine (vaccine risk). the spontaneous risk varies both according to the virus type and according to various factors which determine host resistance. well known among these are: the intrinsic characteristics of the population at a given period (genetic factors and previous experiences of this population with poliomyelitis viruses); env ... | 1976 | 188083 |
| cell surface antigens induced by herpes simplex virus (hsv). | among the antigens induced by hsv on the surface of infected cells there are antigens binding anti-hsv antibodies by their combining (fab) site (hsv-ag), and others binding antibodies of various specificities, probably through their fc fragment (igr). hsv-ag were demonstrated by cytotoxicity and mixed agglutination tests; igr were made evident by passive hemadsorption. the ability to induce igr is especially characteristic of hsv-1 strains, being absent or limited with hsv-2 strains. igr were no ... | 1976 | 188230 |
| isolation of apparently wild strains of poliovirus type 1 from sewage in the ottawa area. | in the first 4 months of 1974, 140 gauze pad samples of sewage collected in the ottawa area were analysed by the bs-c-1 cell system for the presence of viruses pathogenic for humans. viruses were isolated from 111 (79%) of the samples. of the 72 (65%) isolates identified by serology and electron microscopic examination, 56 (78%) were reoviruses and 16 (22%), enteroviruses. the enterovirus isolates included one coxsackievirus b4, one vaccine strain of poliovirus type 3, nine vaccine strains of po ... | 1977 | 188533 |
| control of peptide chain initiation in uninfected and virus infected cells by membrane mediated events. | initiation of protein synthesis in tissue culture cells is rapidly inhibited or blocked by addition of either dmso, ethanol, tpck, cytochalasin b, or sucrose to the growth medium. in contrast, these agents do not interfere with the initiation of protein synthesis in cell-free extracts to a comparable extent. these results support the hypothesis that protein synthesis in tissue culture cells can be influenced by membrane mediated events. translation of viral mrna in rna virus infected cells is re ... | 1976 | 188735 |
| growth characteristics of acute hemorrhagic conjunctivitis (ahc) virus in monkey kidney cells. ii. temperature sensitivity of the isolates obtained at various epidemic areas. | acute hemorrhagic conjunctivitis (ahc) virus strains isolated in eight different areas during epidemics of ahc were tested for the reproductive capacity at 33, 37 and 39 degrees. all of the 25 strains tested grew better at 33 degrees but restrictively at 39 degrees. the degree of temperature sensitivity varied slightly from one strain to the other, but generally exceeded that of attenuated poliovirus type 1, strain lsc2ab. temperature-resistant clones were selected by repeated passages of origin ... | 1976 | 188780 |
| a note on single - and two - dose immunogenicity tests of inactive virus vaccine in chicks. | single dose extinction limit test of inactive polio vaccine in chicks lasts 4 days and offers the same results as the two-dose test which requires 14 days. when "long-term" incubation instead of "conventional" 2 hours-incubation of the serum/virus mixtures in the neutralization reaction was used, extinction limit values higher by 0,6-0,7 log 10 were regularly obtained. | 1976 | 188947 |
| inhibition of host cell protein synthesis by uv-inactivated poliovirus. | the ability of poliovirus that was irradiated with uv light at energies up to 2,160 ergs/mm2 to subsequently inhibit host cell protein synthesis was measured. the inactivation of the host cell shutoff function followed one-hit kinetics. increasing irradiation did not affect the rate of inhibition until the multiplicity of infection after irradiation was reduced to approximately 1 pfu/cell. at higher functional multiplicities, the rate was unchanged, but an increasing lag before the onset of inhi ... | 1977 | 189067 |
| possible in vitro repair of viral rna by ligase-like enzyme(s) in poliovirus-infected cells. | a soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive utp into trichloroacetic acid-insoluble rna linearly for 8 h in the presence of atp and mg2+. radioactive ctp or gtp was not incorporated under identical conditions. nearest-neighbor analysis of the in vitro product demonstrated that atp was added to the viral rna in the form of polyadenylic acid; utp was added internally to the 3'-oh group of all four nucelotides. the data can best ... | 1977 | 189080 |
| 5' termini of poliovirus rna: difference between virion and nonencapsidated 35s rna. | poliovirus cytoplasmic, nonencapsidated 35s rna yields approximately one pup per molecule upon t2 rnase digestion, indicating that this rna has the same 5' end as the polyribosome-associated viral rna fraction. double-stranded, replicative form rna after the same treatment yielded approximately four pnp structures per molecule, 65% of which was pup. in contrast, the 35s rna from mature virions contained no detectable pnp, indicating that the 5' end of the virion rna is different from that of the ... | 1977 | 189096 |
| [selection of virulent poliovirus strains in developing countries? a hypothesis]. | 1992 | 189233 | |
| a protein covalently linked to poliovirus genome rna. | poliovirion [32p]rna, after digestion with rnase t2, yields mononucleotides and a labeled compound "x," which is not negatively charged at ph 5. x contains, relative to the label in virion rna, one to two phosphates and is partially acid insoluble. it can be labeled with tritiated amino acids 3 hr after infection, is insoluble in chloroform/methanol, and can be digested with pronase. these observations suggest that x is a protein. the protein cannot be removed from the polio genome when the rna ... | 1977 | 189316 |
| persistence of poliovirus 1 in soil and on vegetables grown in soil previously flooded with inoculated sewage sludge or effluent. | land disposal of sewage sludge and effluent is becoming a common practice in the united states. the fertilizer content and humus value of such wastes are useful for agricultural purposes, and the recycling of sewage onto the land eliminates many of our stream pollution problems. the potential exists for crops grown in such irrigated soil to be contaminated by viruses that may be present in the sewage. studies were initiated to determine viral persistence in soil and on crops grown under natural ... | 1977 | 189685 |
| poliovirus aggregates and their survival in water. | inactivation of aggregated poliovirus by bromine is characterized by a continuously decreasing reaction rate. poliovirus released from infected cells in these experiments by alternate freezing and thawing in water without electrolytes has always been aggregated. the aggregates persist even on 7,000-fold dilution in ion-free water. virus similarly released into phosphate-buffered saline solution may be well dispersed, but it aggregates when sedimented into a salt-free sucrose gradient or when it ... | 1977 | 189686 |
| survival of some viruses in the sea-water samples collected from the gulf of gdańsk. preliminary studies. | 1976 | 189869 | |
| the size and location of the poly(a) tract in emc virus rna. | encephalomyocarditis (emc) virus rna, selected by its affinity for oligo(dt)-cellulose, contains poly(a) of size : (i) about 14 nucleotide residues long, based on the percentage of radioactivity in the rna resistant to digestion by a mixture of pancreatic and t1 rnases; (ii) about 15 residues long, as measured by the ratio of the amount of terminal adenosine to internal adenylic acid in isolated poly(a); and (iii) in the range 12 to 45 residues, the majority of tracts being about 16 to 18 residu ... | 1977 | 190347 |
| iodination of poliovirus capsid proteins. | 1977 | 190350 | |
| the genome of poliovirus is an exceptional eukaryotic mrna. | 1976 | 190646 | |
| [viral flora (coliphages and human enteroviruses) found in river water after an urban district (saint-etienne). ii. a virological and epidemiological survey (author's transl)]. | a survey was carried out from march 1972 to february 1973 to identify viral flora found in the river furan ater st. etienne. the 54 samples examined revealed the following data: 1) more accurate results are obtained when viral concentration values are expressed in terms of m3/sec., taking into account the flow of the river and eliminating the seasonal dilution factor; 2) rates of enteroviruses remain constant throughout the year, in spite of a relatively rapid spontaneous inactivation of the vir ... | 1976 | 190654 |
| [microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses]. | the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ... | 1976 | 190825 |
| [rational method of producing sera with high titers of virus neutralizing antibodies]. | a possibility of obtaining the diagnostic specific sera with a high titre against the poliovirus, type i, was studied experimentally. five different immunization schemes by the same antigen were tested in parallel experiments. a method of the viral antigen (both of the concentrated and of the unconcentrated) mixed with complete freund's adjuvant injection into the popliteal lymph nodes with the subsequent single reimmunization proved to be most effective. in this way it was possible to obtain ty ... | 1977 | 190829 |
| [ultrastructural changes in the central nervous system of monkeys in poliomyelitis]. | the ultrastructure of cells of the central nervous system of rhesus monkeys in experimental poliomyelitis caused by virus of poliomyelitis type 3 was studied. it was found out that the virus of poliomyelitis was localized in nerve cells, astocytes, oligodendrocytes, the endothelium of capillaries and macrophages. this justifies the conclusion that pathological changes in the cells referred to the above result from reproduction of the virus therein. ultrastructural changes in infected cells are d ... | 1976 | 190980 |
| [action of emetine hydrochloride in some experimental viral infections]. | the authors tested the virulicidal effect of emetine hydrochloride (eh) observed by fusillo in clinical practice. by adding eh to he p-2 cellular system, in a concentration such as to cause some inhibition of cellular growth, there is no meaningfull virostatic or virulicidal action against poliovirus, mahoney's strain. eh, in the same proportion as that employed by fusillo in human therapy, damages in the albino mouse liver. by halving the concentration of eh such a damage is not evident but no ... | 1976 | 191045 |
| seroimmunity to polioviruses in an urban population of italy. | in a seroepidemiological survey on immunity to polioviruses, carried out in rome, neutralizing antibodies were titrated on 602 serum samples collected from individuals aged from 6 months to 88 years. the geometric mean titres were 25.24, 25.92, and 18.12 for poliovirus types 1, 2, and 3, respectively. the number of seronegative results for each virus strain was very low (</=1.5%). the immune status of the study population could therefore be considered satisfactory. the distribution of antibody r ... | 1976 | 191208 |
| jc virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships. | jc virus, a human polyomavirus, failed to grow or produce cytopathic effects in any of a variety of cells tested other than primary human fetal glial (phfg) cells. cells tested included other primary human cells and glial cells from other animals. only a rare cell in inoculated insusceptible human cell cultures produced t or virion antigen. in phfg cell cultures jc virus produced subtle cytopathic effects, and the majority of progeny remained cell associated. only a few cells in the heterogenous ... | 1977 | 191404 |
| the persistence of poliovirus of poliovirus in activated sludge treatment. | a model activated sludge treatment plant was used which was functionally very similar to a full scale plant. it was inoculated with poliovirus either continuously or with a single dose and the amounts of virus in the influent, mixed liquor and effluent were monitored regularly. the distribution of the virus in the liquid and solid phases of the mixed liquor was very unequal with about 85% of the virus associated with the suspended solids fraction. only small amounts of virus were recovered from ... | 1977 | 191521 |
| covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate rnas of poliovirus. | the 5'-terminus of poliovirus polyribosomal rna is pup. a candidate for the 5'-terminus of poliovirion rna was recovered as a compound migrating toward the cathode when 32p-labeled virion rna was completely digested with ribonucleases t1, t2 and a and analyzed by paper ionophoresis at ph 3.5. treatment with proteinase k reversed its direction of migration, indicating the presence of protein. treatment with venom phosphodiesterase liberated all of the radioactivity as pup, suggesting that poliovi ... | 1977 | 191841 |
| importance of associated viral and bacterial infections in the production of acute pneumonias. | bacteriologic and virologic investigations carried out in a group of 85 patients with acute pneumonia have allowed the determination of the etiologic agent in 52.9% of the cases. of these, 40% showed combined viral and bacterial infections. mixovirus infections were frequently associated with staphylococcic ones (7 out of 8 cases) and adenovirus infections with the pneumococcic ones (6 out of 7 cases). the most severe and longdrawn clinical pictures with frequent pleuropulmonary complications (i ... | 1976 | 191900 |
| virological studies of amyotrophic lateral sclerosis: an overview. | 1976 | 191964 | |
| poliovirus antibody status after vaccination with trivalent oral vaccine. | 1976 | 192040 | |
| inactivation of polioviruses and coxsackieviruses in surface water. | inactivation rates of polioviruses 1 and 3 and coxsackieviruses a-13 and b-1 were determined in situ in the rio grande in southern new mexico, using membrane dialysis chambers. inactivation of the viruses was exponential, and the rates of inactivation were apparently affected principally by the water temperature. stability of the viruses in river water differed, with poliovirus 1 and coxsackie b-1 being most stable. typically 1-log reductions of infectivity at water temperatures ranging between ... | 1977 | 192146 |
| [denaturation maps of the replicative form of rna of 2 picornaviruses]. | 1977 | 192525 | |
| the influence of bacillary dysentery on the efficiency of oral poliovaccine in egypt. | the rate of poliovirus excretion was found to be 64.6% in a group of 108 normal children and 11.95% in 184 diarrheal children. diarrhea was due to shigella in 70% of cases. this drew our attention to the presence of an etiologic relationship between the two findings, which may have a direct effect on the low efficiency of oral poliovaccine in our country. effect of shigella infection on the take of oral poliovaccine was investigated in 14 normal and 10 children having acute diarrhea due to shige ... | 1976 | 192625 |
| virological and serological studies of neurological complications of acute hemorrhagic conjunctivitis in thailand. | an extensive outbreak of acute hemorrhagic conjunctivitis (ahc) occurred from september to december 1974 in thailand. at least 29 patients with polio-like motor paralysis that complicated ahc were hospitalized in bangkok. paired or triplicate samples of serum from 16 patients were tested for neutralizing antibody to enterovirus type 70 (ev70). a significant rise in titer of antibody was found for two patients, and the other 14 had neutralizing antibody titers ranging from 1:8 to 1:512 without an ... | 1977 | 192809 |
| differences in the physical properties of dense and standard poliovirus particles. | dense poliovirus particles (dp) differ in buoyant density, sedimentation coefficient and lability from standard poliovirus particles. dense particles band at a density of 1-44 g/ml in isopycnic cscl gradients and sediment in sucrose gradients at 220s. however, when dp are centrifuged in sucrose gradients containing 1-5 m-kcl, nacl or licl, two types of particles are observed, one sedimenting at 220s and the other at 160s. particles sedimenting at 220s are converted into particles sedimenting at ... | 1977 | 192836 |
| [use of a microtechnic for the serological intratype identification of poliovirus strains 1 and 3]. | 1977 | 193175 | |
| isoelectric focusing and 2d-analysis of poliovirus proteins. | 1977 | 193275 | |
| use of elution marker for the intratypic characterization of poliovirus strains. | elution marker was used for intratypic characterization of poliovirus strains with al(oh)3 gel as adsorbent. the virion suspensions to be tested were partially purified by chromatography and labelled with 32p. in the labelled preparations of wild virus strains practically all radioactivity was found in virus-specific bond, whereas in those of the vaccine strains and isolates of vaccine origin a considerable, but variable, proportion of the activity was bound to residual cell components. for this ... | 1976 | 193364 |
| enteroviruses other than poliovirus. | the role of enteroviruses in certain specific disease--cardiac disease, nephritis, diabetes, and hemorrhagic conjunctivitis--is examined. it has now been well documented that coxsackievirus b (types cb1 through cb5 but not cb6) is the main pathogen involved in various clinical forms of viral heart disease. coxsackievirus a (ca4 and ca16) and echovirus (types 9 and 22) may also be associated with viral heart disease. in regard to the etiologic role of enteroviruses in nephritis, pancreatitis, and ... | 1976 | 193602 |
| antibody production and complement system in protein energy malnutrition. | antibody production in kwashiorkor and marasmic infants was studied by dividing them into three groups and giving the first group a single dose of trivalent live attenuated polio virus, the second group live attenuated measles virus and the third group diphtheriatoxoid. the production of antibodies was found to be deficient in pem as compared to normals and diminished more in kwashiorkor than in marasmus cases. the individuals of the complement system were significantly lower in kwashiorkor than ... | 1977 | 194050 |
| detection of poliovirus antigens and antibodies: microindirect haemagglutination and haemagglutination inhibition tests for poliovirus types i, ii, and iii. | microtitre indirect haemagglutination (iha) and iha-inhibition (ihai) procedures were adapted to determine the reactivities of type i, ii, and iii poliovirus antibodies and antigens. glutaraldehyde-fixed sheep erythrocytes were sensitized for these tests with concentrated, partially purified preparations of type i, ii, and iii poliovirus. antibody titres by iha were generally 10 to 100 times greater than serum microneutralization (sn) titres. the sn and iha reactivities of three kinds of sera we ... | 1976 | 194134 |
| echovirus 12-induced host cell shutoff is prevented by rhodanine. | 1977 | 194165 | |
| the role of some viruses in acute pneumonias in adults. | virological and bacteriological investigations were performed in 85 patients with acute pneumonias and virus isolation or serological evidence of virus infection were obtained in 37.6% of the cases. influenza a2 and b viruses were incriminated in 14.1% of the patients; parainfluenza viruses in 7% and adenoviruses in 17.2% of the cases. coxsackie virus was isolated from one patient's blood, and poliovirus 3 was recovered in 3 cases. in 5 cases associated virus infections were detected. | 1977 | 194393 |
| studies on the in vitro uncoating of poliovirus. ii. characteristics of the membrane-modified particle. | 1977 | 194397 | |
| [virus elimination through purification of drinking water]. | 1977 | 194424 | |
| evolution of poliovirus since introduction of attenuated vaccine. | genetic marker tests were performed on 997 strains of poliovirus isolated from patients with neurological disease and from healthy people in england and wales. before the introduction of live attenuated vaccine most strains could multiply at raised temperatures. now, however, many strains isolated from cases of poliomyelitis or from healthy persons with no known contact with vaccine cannot grow above 37 degrees c, and in this respect resemble the vaccine strains. the three serotypes are also muc ... | 1977 | 194650 |
| chronic progressive poliomyelitis secondary to vaccination of an immunodeficient child. | we investigated an immunodeficient child in whom chronic progressive poliomyelitis developed after she had received live oral poliovirus vaccine. poliovirus, type ii, was isolated from throat and stool during life and from several sites within the brain at autopsy. the brain isolate was classified as vaccine-like on the basis of temperature sensitivity and antigenic markers. however, in the monkey neurovirulence test, the brain isolate produced moderately severe lesions throughout the spinal cor ... | 1977 | 195206 |
| recommendations for a national policy on poliomyelitis vaccination. | declining numbers of adequately vaccinated persons, new data about the comparative safety and effectiveness of live, attenuated and killed poliomyelitis-virus vaccines, increased consumer awareness of adverse reactions and pressure from manufacturers seeking protection from liability were factors leading the institute of medicine to re-examine poliomyelitis vaccination programs. the relative merits of live and killed virus vaccines as immunizing agents were reviewed within the context of the 60 ... | 1977 | 195207 |
| distribution of poliovirus in a small community. | 1977 | 195253 | |
| antibody persistence after primary immunization with trivalent oral poliovirus vaccine. | five years after primary infant immunization with trivalent oral poliovirus vaccine, employing either a three-dose primary series as recommended by the u.s. public health service advisory committee on immunization practices (acip) or a four-dose series as recommended by the committee on infectious diseases of the american academy of pediatrics. 115 children were serologically tested for persistence of neutralizing antibodies by the microneutralization test. of the 57 individuals immunized accord ... | 1977 | 195265 |
| seroepidemiology of the poliovirus in monrovia (liberia). | neutralizing antibodies against poliovirus have been studied in 265 persons in monrovia (liberia). 34% of the individuals studied possessed antibodies against the three polioviruses, 23% against two, 26% against one; the remaining 17% showed none. the three poliovirus antibodies were evenly distributed amongst the population, although there was an indication that antibodies to polio 2 occurred more commonly in females than in males. the distribution of the antibodies did not seem to be influence ... | 1992 | 195370 |
| comparison of primary rhesus and cynomolgus monkey kidney cell cultures for viral isolation from clinical specimens. | rhesus monkey kidney and cynomolgus monkey kidney cell cultures were compared for viral isolation by using clinical specimens that yielded 203 viral isolates. cynomolgus and rhesus monkey kidney cells were comparable for the isolation of 22 adenoviruses, 12 coxsackieviruses, and one poliovirus. four of 50 echoviruses and seven of ten herpesviruses were detected only in cynomolgus monkey kidney cells. influenza virus was isolated in 84 instances, of which eight were detected only in rhesus and fo ... | 1977 | 195459 |
| inactivation of poliovirus in wastewater sludge with radiation and thermoradiation. | sludge is highly protective of poliovirus against both heat and ionizing radiation. this protection could be largely overcome when these two treatments were given simultaneously. | 1977 | 195518 |
| stimulation of synthesis of the proteins of 30-s nuclear ribonucleoprotein particles in human amnion u cells by viral infection. | early increase in rna synthesis induced in human amnion u cells by infection with poliovirus is accompanied by an increased incorporation of amino acids into non-histone nuclear proteins with an approximate molecular weight of 40 000. these proteins are the main polypeptides of the 30-s nuclear ribonucleoprotein particles. after fractionation of nuclear proteins by extraction with solutions of different ionic strength, these polypeptides are present in the fraction of nuclear sap proteins solubl ... | 1977 | 195613 |
| morphogenesis of poliovirus. iv. existence of particles sedimenting at 150s and having the properties of provirion. | an apparent precursor to the poliovirion that cosediments with the virion at 150s was identified by its content of vp-0. it has properties previously associated with the provirion, a structure that sedimented at 125s, and it may be an alternate form of provirion. like virions, the 150s precursor binds to and elutes from cells, after which it sediments at about 125s. | 1977 | 196114 |
| the location of the polio genome protein in viral rnas and its implication for rna synthesis. | evidence is presented that a small protein (vpg) is covalently attached to the 5'-terminal oligonucleotide vpg-pu-u-a-a-a-a-c-a-gp of the polio genome, to nascent strands of the polio replicative intermediate and to poly(u) of minus strands. a model of polio rna replication is proposed implicating vpg in initiation of rna symthesis, possibly as primer. | 1977 | 196204 |
| 5'-terminal nucleotide sequences of polio virus polyribosomal rna and virion rna are identical. | 1977 | 196211 | |
| untranslated vesicular stomatitis virus messenger rna after poliovirus infection. | 1977 | 196392 | |
| poliovirus coat protein as the site of guanidine action. | 1977 | 196400 | |
| [genetic analysis of the poliovirus]. | 1977 | 196449 | |
| simple mathematical deductions in the seroepidemiology of viral infections. ii. (para) myxoviruses (measles, mumps, influenza b), rubella, enteroviruses (polio, coxsackie b), adenoviruses, and mycoplasma pneumoniae. | large samples of nonselected persons collected in south-west germany were investigated for the prevalence of serum antibodies to poliovirus 1-3 and coxsackievirus b 1-5 (neutralisation test), to measles, rubella, and mumps (hemagglutination inhibition test), mumps, influenza b, adenovirus, and mycoplasma pneumoniae (complement-fixation test). according to "catalytic models", which compare the infection spread to simple chemical reactions of molecules as self-limiting procedures, a mathematical a ... | 1977 | 196454 |
| [experiments on poliomyelitis surveillance in an urban population of the ruhr valley (essen) by means of virological investigations of the sewage water (author's transl)]. | 1977 | 196458 | |
| differences in multiplication of virulent and vaccine strains of poliovirus type i, ii, and iii in laboratory animals. | multiplication of virulent and vaccine strains of poliovirus type i, ii and iii in laboratory animals of different species was studied comparatively. the main criterion of virus reproduction was the production of the photoresistant virus progeny after inoculation of the animals with proflavin-photosensitized virus strains. on the whole, virulent poliovirus strains were characterized by replication in a wide range of hosts (monkeys, cotton rats, white mice, guinea pigs, rabbits, chickens, chick e ... | 1977 | 196575 |
| separation and quantitation of intracellular forms of poliovirus rna by agarose gel electrophoresis. | intracellular poliovirus-specific rna species can be measured directly by electrophoresis of total cytoplasmic nucleic acids through 1% agarose gels, resulting in the separation of single- and double-stranded forms of poliovirus rna from each other and from hela cell 28s ribosomal rna. single-stranded rna molecules differing by only 15% in length are resolved in this gel system. rna species can be visualized as fluorescen bands appearing after staining of the gels with ethidium bromide and obser ... | 1977 | 196620 |
| enhancement of virus growth produced by thiols and disulphides. | several thiols and disulphides have been found able both to shorten the latency phase and to increase the growth of several virus strains in cell cultures. | 1977 | 196894 |
| chromosomal site(s) of integration of herpes simplex virus type 2 thymidine kinase gene in biochemically transformed human cells. | 1977 | 197027 | |
| comparison of poliovirus detection in sewage and stool samples; a study in a crèche in the third week after vaccination. | in a model experiment conducted in a prague crèche, the detection rate for polioviruses in partially purified, non-concentrated sewage as sampled by means of gauze pads (moore's method) was compared with that for individual stool samples. in the third week after the vaccination of children with live attenuated type 1 poliovirus (march 1975) and at an equal interval after their vaccination with a combination of types 2 and 3 (may 1975), corresponding poliovirus types were detected both in individ ... | 1977 | 197033 |
| vaccine-associated poliomyelitis in a child with sex-linked agammaglobulinemia. | paralytic poliomyelitis was observed in a child with a sex-linked defect in immunoglobulin synthesis. evidence is presented that this was secondary to administration of oral, live poliovaccine. the demonstration of a familial sex-linked gammaglobulin deficiency and the failure to document a defect in cell-mediated immunity in this child extends the risk of vaccine associated poliomyelitis to virtually all forms of immunodeficiency. the critical host factors in the pathogenesis of poliovirus vacc ... | 1977 | 197220 |
| correlation of virus polypeptide structure with attenuation of poliovirus type 1. | several avirulent samples of poliovirus type 1 derived in the process attenuating the neurovirulent mahoney strain show an altered virus capsid polypeptide, vp-1, on polyacrylamide gel electrophoresis of sodium dodecyl sulfate-disrupted virions. | 1977 | 197272 |
| radiation enhanced reactivation of nuclear replicating mammalian viruses. | 1977 | 197551 | |
| a plasma membrane component able to bind and alter virions of poliovirus type 1: studies on cell-free alteration using a simplified assay. | 1977 | 197703 | |
| [cytochalasins in virologic research]. | 1977 | 197711 | |
| [several virologic and biochemical aspects of vaccination with antipoliomyelitis vaccine. fate of the parent virus]. | in the present study we attempted to follow the fate of the parental poliomyelitis virus of immunological type ii labeled with 3h-uridine in vaccinated animals. for this purpose, 3h-uridine labeled purified and concentrated virus was inoculated intramuscularly into cotton rats (sensitive to poliomyelitis virus) and guinea pigs (insensitive to poliomyelitis virus). the controls consisted of two groups of the same animal species: the animals of the first group were inoculated intramuscularly with ... | 1977 | 197713 |
| [several virologic and biochemical aspects of vaccination with antipoliomyelitis vaccine. characteristics of intracellular virus-specific structures]. | the data are presented concerning infectious potentials of subvirus particles isolated from cytoplasmic extracts of various organs of cotton rats and guinea pigs inoculated intramuscularly with purified and concentrated poliomyelitis virus type ii. as described earlier, from various organs of the animals (the brain, spinal cord, lungs, and spleen) subvirus particles were isolated which in cesium chloride gradient had a density higher by 0.05-0.1 g/ml than that of the intact virus. determinations ... | 1977 | 197714 |
| evolution of poliovirus since introduction of attenuated vaccine. | 1977 | 198059 | |
| virus detection on grapes. | grapes inoculated with poliovirus 1 and coxsackievirus b5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. these washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. | 1977 | 198080 |