Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [correlation of serous meningitis morbidity with the prevalence of enteroviruses in water]. | investigation of the relationship between the incidence of aseptic meningitis etiologically due to coxsackie b1 virus and circulation of enteroviruses in the water indicates not only a correlation between enteroviruses isolation from sewage and from patients, but also a direct relationship between the number of virus excretors and prevalence of enteroviruses in open water bodies in respect of seasonal distribution, type composition, frequency of detection and concentration of viruses in differen ... | 1988 | 2842964 |
| [effect of coxsackie a13 and b3 virus infections on the primary immune response to sheep erythrocytes]. | inbred balb/c mice of various ages (1 1/2, 3, and 5 months) inoculated with different doses of coxsackie b3 virus showed reduced antibody responsiveness after immunization with sheep red blood cells 3-10 days after the primary infection. reduced antibody response exhibited by spleen cells was observed in 7-month-old mice infected with coxsackie a13 virus 10-11 days after infection. | 1988 | 2842965 |
| novel approach for modifying microporous filters for virus concentration from water. | electronegative microporous filters composed of epoxyfiberglass (filterite) were treated with cationic polymers to enhance their virus-adsorbing properties. this novel and inexpensive approach to microporous filter modification entails soaking filters in an aqueous solution of a cationic polymer such as polyethyleneimine (pei) for 2 h at room temperature and then allowing the filters to air dry overnight on absorbent paper towels. pei-treated filters were evaluated for coliphage (ms2, t2, and ph ... | 1988 | 2843091 |
| coxsackievirus-cell interactions that initiate infection in porcine ileal explants. | coxsackievirus b5 (cb5) labeled with tritiated uridine was used to trace the interaction of the virus with explant cultures of porcine ileum. similarly labeled human poliovirus 1 (po 1), which is not specifically retained by porcine tissue, was used as a control. the explant procedure employed could maintain ileal tissue in a differentiated state for up to 48 hours. porcine ileum was acquired from both young (4-6 week-old) and adult (9-11 month-old) animals. inoculated explants of either absorpt ... | 1988 | 2843148 |
| hydrops due to myocarditis in a fetus. | at 18 weeks of gestation a fetus was studied sonographically because of advanced maternal age and found to have hydrops of unknown etiology with ascites, pleural, and pericardial effusions. an abortion was performed and in the fetal/placental material myocarditis and thyroiditis were documented. maternal antibodies to coxsackie virus b5 showed a onefold rise. tentatively, the hydrops is assigned to coxsackie virus myocarditis, a lesion not previously identified in fetuses. | 1987 | 2843206 |
| flow-cytometric analysis of lymphocyte subsets in relation to virus infections at the onset of type 1 (insulin-dependent) diabetes. | alterations in t-lymphocyte subsets have been connected to the autoimmune pathogenesis of type 1 (insulin-dependent) diabetes. in this study peripheral blood lymphocytes were analysed by flow cytometry using okt3, okt4, okt8, anti-hla-dr, anti-il-2 receptor and anti-membrane immunoglobulin antibodies in newly diagnosed type 1 diabetic children, their healthy siblings and healthy control children. the results were compared to the occurrence of serologically verified recent virus infections, some ... | 1988 | 2843209 |
| effect of coxsackievirus b4 infection in mice on expression of 64,000-mr autoantigen and glucose sensitivity of islets before development of hyperglycemia. | diabetogenic strains of coxsackievirus b4 (cb4) produce a diabetes syndrome in susceptible mice that resembles insulin-dependent diabetes mellitus. to assess the possible role of autoimmunity, the expression of a 64,000-mr islet antigen in sjl/j and cd1 mice infected with a diabetogenic strain of cb4 was monitored in early and late infection. additionally, virus-induced abnormalities in glucose metabolism were correlated with several changes in purified islets to assess beta-cell physiology. ove ... | 1988 | 2843410 |
| type b-2 coxsackievirus meningitis in pregnancy. a case report. | although enteroviral meningitis complicating pregnancy is a rare occurrence, it can lead to serious antenatal and neonatal complications. the majority of neonatal enteroviral infections are transmitted from a perinatally infected mother. we treated a woman for type b-2 coxsackievirus meningitis diagnosed in pregnancy. three weeks after the acute maternal infection was diagnosed, a preterm neonate with no evidence of enteroviral infection was delivered. the neonate had no detectable igm or virus- ... | 1988 | 2845075 |
| coxsackie b3 virus: an unusual cause of unilateral mediastinal nodal enlargement. | we report on a patient with febrile illness, right unilateral paratracheal widening, pulmonary infiltrates, pleural effusions, exanthema and diarrhea. the right paratracheal widening was due to lymph node enlargement confirmed by ct scan. symptoms disappeared subsequently without specific treatment. on the basis of serological tests, we are able to diagnose a coxsackie b3 virus infection. no previous case report of unilateral mediastinal nodal enlargement contemporary to a coxsackie b3 virus inf ... | 1988 | 2845536 |
| coxsackievirus b-3 selection of virus resistant buffalo green monkey kidney cells and chromosome analysis of parental and resistant cells. | following coxsackievirus b-3 (cbv-3) infection and lysis of highly susceptible buffalo green monkey kidney (bgmk) cells, there was a regrowth of cells. cultures of regrown cells were established and they continued to release infectious cbv-3 for up to 20 weeks. the parental bgmk cells were susceptible to cbv-3, cbv-4 and poliovirus type 2 induced cytopathic effect (cpe), while the cured cells were resistant to cbv-3 and cbv-4 but not to poliovirus type 2. attachment of cbv-3 was restricted on cu ... | 1988 | 2845893 |
| purification and characterization of a strain of coxsackievirus b4 of human origin that induces diabetes in mice. | a diabetogenic strain of coxsackievirus b4 of human origin has been purified to study its biochemistry and diabetogenicity. tissue culture cells infected with the virus contain two distinct types of particles--virions and membrane-bound virions (mbv). mbvs are lighter (p = 1.29) than virions (p = 1.34), and they contain relatively more protein than rna. virons contain four capsid proteins, vpi-4, of various molecular weights: vp1, 37,500; vp2, 36,000; vp3, 26,000; and vp4, 5,500. mbvs contain th ... | 1988 | 2846770 |
| the role of coxsackie b viruses in the pathogenesis of myocarditis, dilated cardiomyopathy and inflammatory muscle disease. | coxsackie b viruses are members of the family picornaviridae which have been associated by retrospective serology with a range of muscle diseases, particularly myocarditis, dilated cardiomyopathy and epidemic pleurodynia (epidemic myalgia or bornholm disease). it has been proposed that virus-induced myocarditis disposes to the development of idiopathic dilated cardiomyopathy. however, despite many attempts, isolation of infectious virus or immunofluorescent detection of virus-specific antigens i ... | 1987 | 2847741 |
| a murine model of acute myocarditis infected by coxsackie virus b3m. | 1988 | 2847902 | |
| pathogenesis of acute myocardial necrosis in inbred mice infected with coxsackievirus b3. | the pathogenesis of myocardial necrosis due to cb3w infection was studied in balb/c and c3h/hej mice. balb/c mice infected with 5 x 10(4) pfu were found to die of massive hepatic coagulative necrosis before myocardial changes occurred. reducing the inoculum size to 5 x 10(2) pfu resulted in sublethal hepatic involvement and multifocal myocardial coagulative necrosis by day 7 p.i. in contrast, c3h/hej mice survived infection and developed multifocal myocardial coagulative necrosis, but not liver ... | 1988 | 2848174 |
| [diabetogenic properties of coxsackie a13 and coxsackie b4 viruses in experimental infection in mice]. | studies of diabetogenic properties of coxsackie a13 and b4 viruses in mice sensitive to diabetes (males, dba line) and resistant (males and females f1(cba x c57bl/6), females dba/2 using in the latter case the subdiabetogenic doses of alloxan revealed in the infected animals biochemical changes manifested by reduction of glucose tolerance and disorders in the synthesis of immunoreactive insulin. most marked changes were observed in males of dba/2 line infected with coxsackie b4 virus and in male ... | 1988 | 2848368 |
| [diagnosis of viral myocarditis by enzyme-linked immunosorbent assay (elisa)]. | 1988 | 2848674 | |
| cardiovascular effects of congenital infections. | in the course of gestation, many bacteria, parasites, and viruses may infect the pregnant woman, but few cross the placenta to affect the fetus and fewer still affect the fetal heart. although the incidence of fetal cardiac infection is low, the effect on the fetus is major. in terms of frequency, rubella virus, toxoplasma gondii, and coxsackie virus b are the principal infectious agents affecting the fetal heart, but any number of organisms may cross the placenta to affect the fetus. the pathog ... | 1987 | 2849958 |
| a highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and tween 80. | addition of tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. when reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. this floating layer can be obtained with several nonionic detergents including tween 80 and under a salt saturation of 55% with ammonium sulfate, potassium tartrate, and sodium phosphate. virus recovery ranged fr ... | 1988 | 2850100 |
| presence of insulin binding sites on viral particles. | using a standard radio-receptor assay, we have demonstrated that [125i]insulin can bind specifically to each of two types of purified enveloped viruses, influenza a virus and rous sarcoma virus. a non-enveloped icosahedral virus (echovirus 11) and herpes simplex virus type 2, which acquires its envelope from the nuclear membrane of the cell, did not possess insulin receptor activity. displacement of specifically bound radiolabelled insulin from the viral surface was achieved by addition of an ex ... | 1988 | 2851292 |
| [clinical significance of the spontaneous change in serum viral antibody titers in patients with acute coxsackie b virus myocarditis]. | 1988 | 2851423 | |
| virus infections and sports performance--a prospective study. | there are numerous anecdotal reports relating infection to deterioration in sporting performance. unexplained failures by top sportsmen are often attributed to recent or current infections. we have carried out a prospective investigation to determine the effect of viral infections on the performance of a group of 68 elite track and field athletes. athletes were monitored for evidence of viral infection during winter training and their form was assessed subjectively and also semi-objectively by a ... | 1988 | 2852528 |
| comparison of cell cultures for rapid isolation of enteroviruses. | cell culture isolation is still the most reliable method for the detection of enteroviruses from clinical specimens. rapid diagnosis of enterovirus infection affects patient management. to increase yield and enhance the rapidity of enterovirus isolation in cell cultures, we used buffalo green monkey kidney (bgm) cells and subpassages of primary human embryonic kidney (hek) cells in addition to the human diploid fibroblast (mrc-5) cells and primary cynomolgus or rhesus monkey kidney (mk) cells ro ... | 1988 | 2852672 |
| the anti-inflammatory effect of ls 2616 and poly i:c in coxsackievirus b3 induced murine myocarditis. | we have studied the effects of immunotherapy in coxsackievirus b3-induced myocarditis in male balb/c mice. a single i.p. injection of the synthetic interferon inducer poly i:c conferred an almost total protection from lethality when administered at 0 h or 24 h after infection. poly i:c treatment at 48 h after infection, as well as daily i.p. injections of the quinoline-3-carboxamide ls 2616, a new stimulator of nk-cell activity, gave no protection from lethality. the inflammatory lesions and nec ... | 1988 | 2852917 |
| [the role of coxsackie viruses in the pathogenesis of diffuse toxic goiter]. | altogether 15 untreated patients aged 16 to 55 with diffuse toxic goiter had morphologically confirmed diagnosis of the presence of antibodies to coxsackie virus (group b); titers of group a (1:128 and more) were revealed in 3 of them. it indicated recent (not more than 2 yrs.) coxsackie virus infection. such titers were absent in the control group (18 healthy persons). difference in the study and control groups was statistically significant (p less than 0.05). the role of coxsackie virus in the ... | 1988 | 2853353 |
| [indices of the interferon system in patients with viral myocarditis and dilated cardiomyopathy]. | the authors presented some data on the investigation of the interferon system in patients with dilatation cardiomyopathy (dcm) and viral myocarditis (vm). diagnosis was verified according to the who criteria. altogether 10 dcm patients and 24 vm patients with a grave course were investigated. antibodies to coxsackie b virus and influenza were detected in the vm patients. a decrease in the alpha- and gamma-interferon activity was shown in all dcm patients. the vm patients with a grave course also ... | 1988 | 2853455 |
| igm against coxsackie b viruses in children developing type i diabetes mellitus--a seven-year retrospective study. | igm antibodies to coxsackievirus type b 1-5 (cb 1-5) have recently been observed in sera from children with newly diagnosed insulin-dependent diabetes mellitus (iddm). in the present study iddm patients below 15 years of age diagnosed between 1978 and 1984 inclusive in two different areas (counties) of sweden, uppsala and linköping, were studied at the onset of their disease. the incidence of iddm per 100,000 children below the age of 15 years varied between 11.1 and 40.8, with a mean of 26.3 in ... | 1988 | 2854031 |
| detection of enteroviruses using subgenomic probes of coxsackie virus b4 by hybridization. | the objective of this research was to develop group- and type-specific probes for the detection of enteroviruses. coxsackie virus b4 rna was cloned, and a series of subgenomic clones were generated. six of these clones, containing sequences from the 3' end or the 5' end of the genome, were tested for their ability to detect these viruses in a small number of infected cells employing nucleic acid hybridization technique and total cytoplasmic rna from a panel of 11 serotypes of enteroviruses. the ... | 1988 | 2854510 |
| [isolation of immunoglobulins from the egg yolk of hens immunized with intestinal viruses]. | antibodies from the yolk of eggs of chicken immunized with enteric viruses (simian rotavirus sa 11, poliomyelitis virus type 2, and coxsackie b2) were obtained. the time course of the emergence and amplification of antibody in the yolk was followed for several months by neutralization tests and enzyme immunoassay. the long-term persistence of a high antibody level in the yolk, the simplicity of generation of large amounts of chromatographically pure preparations, as well as specificity of chicke ... | 1988 | 2854680 |
| [the virus content of running water (the aare river) in switzerland]. | 1988 | 2854914 | |
| prevention of murine coxsackie b3 viral myocarditis and associated lymphoid organ atrophy with recombinant human leucocyte interferon alpha a/d. | the effects of recombinant human leucocyte interferon alpha a/d on experimental myocarditis due to coxsackie virus b3 were investigated. specific plaque reduction assays showed that 50% of in vitro plaque formation in vero (kidney of african green monkey) cells was inhibited by interferon alpha a/d 48 u.ml-1 when administered 24 h before infection with coxsackie virus b3. three week old male c3h/he mice were inoculated intraperitoneally with 3 x 10(2) plaque-forming units (pfu) of coxsackie viru ... | 1988 | 2855719 |
| coxsackie b, mumps, rubella, and cytomegalovirus specific igm responses in patients with juvenile-onset insulin-dependent diabetes mellitus in britain, austria, and australia. | patients from england, austria, and australia with recently diagnosed juvenile-onset insulin-dependent diabetes (type 1) mellitus (iddm) and matched controls were tested for specific igm responses to coxsackie b1-5 viruses. 37 of 122 (30%) patients aged less than 15, but only 15 of 204 (6%) controls, were positive (p less than 0.005). differences in coxsackie b virus specific igm responses between patients and controls were statistically significant for patients in england and austria (p less th ... | 1985 | 2861361 |
| insulin, glucagon and somatostatin content of the non-obese diabetic (nod) mouse pancreas and plasma virus antibodies to coxsackie b- and reoviruses. | the amounts of insulin, glucagon and somatostatin in the pancreas of nod mice were determined and the results were compared with those of normal icr-strain mice, and plasma antibodies to coxsackie b-3 and reovirus types 1, 2 and 3 were measured. in the pancreas of nod mice with fasting plasma glucose (fpg) less than 140 mg/100 ml, the insulin content of the male mice was similar to that of the normal controls, ranging to 3.55 +/- 0.31 u/g wet weight of pancreas, but it was already significantly ... | 1985 | 2862717 |
| coxsackie b virus and juvenile-onset insulin-dependent diabetes. | 1985 | 2863487 | |
| coxsackie-b-virus-specific igm responses, complement-fixing islet-cell antibodies, hla dr antigens, and c-peptide secretion in insulin-dependent diabetes mellitus. | to evaluate the role of coxsackie b viruses in the pathogenesis of insulin-dependent (juvenile-onset, type 1) diabetes mellitus (iddm), attempts were made to correlate virus-specific igm responses with hla genes, autoimmune responses, and c-peptide secretion. hla dr3, dr4, or both were present in 73 of 90 (81%) diabetic patients; 22 of 23 (96%) with coxsackie-b-virus-specific igm had at least one of these hla types, compared with 51 of 67 (76%) without virus-specific igm. there was no correlatio ... | 1985 | 2863632 |
| detection of coxsackie-b-virus-specific rna sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy. | full-length virus genomic rna (7.4 kilobases; kb) was isolated from coxsackie b2 virus purified from infected monkey kidney cells in culture. dna complementary to 6.3 kb of virus rna was prepared by reverse transcription and cloned in a plasmid vector. a 1.6 kb coxsackie-b-virus-specific dna clone derived from the conserved 3' region of the virus genome was used as a hybridisation probe to test for the presence of virus nucleic acid sequences in myocardial biopsy samples. positive hybridisation ... | 1986 | 2871380 |
| persistent coxsackie b virus specific igm response in patients with recurrent pericarditis. | 1986 | 2873293 | |
| persistence of coxsackie b virus-specific igm. | 1986 | 2874305 | |
| coxsackievirus b4 heterogeneity: effect of passage on neutralization and mortality. | we have compared two cb4 isolates for virulence, tissue tropism, and antigenic drift using monoclonal antibodies. both isolates replicated in c57b1/6 and balb/c mice. the human isolate edwards, recovered from a fatal case of encephalohepatomyocarditis, produced lethal infection in adult animals. lethal infections were associated with high viral titers in visceral organs but not with the presence of specific neutralizing epitopes. virulence seemed stable upon passage, and also the avirulent jvb i ... | 1986 | 2881467 |
| dermatomyositis, polymyositis, and coxsackie-b-virus infection. | coxsackie-b-virus-specific probes prepared by reverse transcription of purified virus genomic rna and molecular cloning techniques were used in quantitative slot-blot hybridisations to test for the presence of virus rna in skeletal muscle biopsy samples. the samples tested were from two patients with adult polymyositis, seven with juvenile dermatomyositis, four with duchenne muscular dystrophy, and six normal controls. of the nine patients with inflammatory muscle disease, five were positive for ... | 1987 | 2883345 |
| matching of host genotype and serotypes of coxsackie b virus in the development of juvenile diabetes. | thirty-six consecutive paediatric patients (0-16 years old) with recently contracted juvenile diabetes (iddm) during 1982-84 were included in the study. sera were assayed for recent or current coxsackie b virus (cbv) infection using a specific and sensitive igm ria. eighteen patients (50%) had igm against cbv 1-5. the patients were also assayed for restriction fragment length polymorphism (rflp) patterns with dna probes coding for hla-dr and dq beta chains. the cbv-positive patients (n = 18) had ... | 1987 | 2888188 |
| chronic enterovirus infection in patients with postviral fatigue syndrome. | 76 patients with the postviral fatigue syndrome (pvfs) and 30 matched controls were investigated. virus isolation was attempted from concentrated faecal samples by direct culture and after acid dissociation of virus from antibody. positive cultures of enteroviruses were obtained from 17 (22%) patients and 2 (7%) controls. an enterovirus-group-specific monoclonal antibody, 5-d8/1, directed against the vp1 polypeptide, was used to detect enteroviral antigen in the circulation, either free or compl ... | 1988 | 2892990 |
| interaction between enteroviruses and human endothelial cells in vitro. alterations in the physical properties of endothelial cell plasma membrane and adhesion of human granulocytes. | fluorescent molecular probes were used for study of the interaction between enteroviruses (echo 9, echo 12, and coxsackie b3 virus) and human endothelial cells in monolayer culture. with the use of the monomer-excimer method with pyrene decanoic acid it was shown that a marked dose-dependent restructuring of the plasma membrane occurred following addition of virus to the endothelial cells. this took the form of an increase in the lipid surface available to the lipophil reporter molecules, probab ... | 1985 | 2981472 |
| aseptic meningitis in a neonate with an oral vesicular lesion. | enteroviruses may be responsible for severe, life-threatening diseases, such as meningitis, within the neonatal period. differentiation of an enteroviral etiology from that of herpes simplex virus may be difficult initially. we describe a case of aseptic meningitis in which the use of a viral blood culture was helpful. | 1985 | 2981653 |
| nmr parameters of local anesthetics as biological markers of the cell-virus interactions. | the selective proton nmr relaxation rates were measured in the system made by the local anesthetic procaine, human erythrocytes and the hemagglutinating echo virus type 11. the nmr technique provided a very good tool for using drugs firmly bound to receptor membranes as biological "markers" of the virus-cell interaction. | 1985 | 2982355 |
| properties of the deoxycholate-solubilized hela cell plasma membrane receptor for binding group b coxsackieviruses. | physical and chemical properties of deoxycholate-solubilized hela cell plasma membrane receptors for binding group b coxsackieviruses were determined. receptors eluted from sepharose 4b with an apparent molecular weight of 275,000 and sedimented with an s value of between 14.7 and 4.9 and a buoyant density of 1.06 to 1.10 g/cm3. virus-binding activity was destroyed after treatment with proteases, glycosidases, and periodate but was unaffected by lipases or reducing or alkylating agents. addition ... | 1985 | 2983096 |
| invasiveness of salmonella typhimurium in hep-2 cell cultures pretreated with uv-inactivated coxsackie virus. | the invasiveness of salmonella typhimurium was significantly enhanced in cell cultures pretreated with uv-inactivated virus. during the first 3 hours of virus infection there was no difference between the enhancement achieved with non-inactivated and that achieved with uv-inactivated virus. after 4 and 5 hours pretreatment the effect of non-inactivated virus was more pronounced than that of uv-inactivated virus. the results indicate that during the early period of virus infection the enhancement ... | 1985 | 2984878 |
| virion conformational forms and the complex inactivation kinetics of echovirus by chlorine in water. | aberrant inactivation kinetics were observed when monodispersed echovirus type 1 (farouk) was inactivated with chlorine. an initial 1- to 2-log10-unit decrease in titer was followed by lag period, during which the titer stayed the same or increased, and this was followed by a final decline in titer. first-order kinetics were obtained with poliovirus type 1 under the same conditions. isoelectric focusing studies of echovirus before chlorine treatment showed that the virus distributed into two ph- ... | 1985 | 2984991 |
| membrane-bound virions of coxsackievirus b4: cellular localization, analysis of the genomic rna, genome-linked protein, and effect on host macromolecular synthesis. | hela cells infected with several group b coxsackieviruses contain, in addition to standard virions, a population of virus-specific ribonucleoprotein particles which we (5) designated membrane-bound virions (mbv). mbvs differ from standard virions in buoyant density, yield, appearance, protein composition and infectivity. here we present several new features of mbvs of coxsackievirus b4. the mbvs are lighter (rho about 1.30) and are localized in rough membranes, intermixed with virions. they cont ... | 1985 | 2985028 |
| biochemical changes induced by coxsackie b4 virus in short-term culture of mouse pancreatic islets. | isolated mouse pancreatic islets were infected in vitro with two strains of coxsackie b4 virus--a tissue culture-adapted strain and a mouse pancreas-adapted strain. within 48 h of infection changes had occurred in the biochemical activities of islets infected with the mouse pancreas-adapted strain of virus. basal insulin release was increased two-fold in these islets, while glucose-induced insulin secretion remained unchanged. insulin biosynthesis was greatly reduced at a stimulatory concentrati ... | 1985 | 2985140 |
| seasonal distribution of enteroviruses and adenoviruses in domestic sewage. | a seasonal distribution of enteroviruses and adenoviruses in raw sewage effluents of athens, greece, was observed over a 15-month surveillance period. all 36 samples tested were positive for both virus groups. adenovirus concentration levels ranged from 70 to 3200 cytopathic units per litre of sample, whereas the corresponding values for enteroviruses were 90-900 cytopathic units per litre. peak values for adenoviruses were recorded during the months of april and june 1983, whereas for enterovir ... | 1985 | 2985226 |
| ameliorating effect of ifn-beta and anti-ifn-beta on coxsackievirus b3-induced myocarditis in mice. | a significant reduction in the number of virus-induced myocardial lesions was effected by administration of murine interferon beta (ifn-beta) or polyriboinosinic: polyribocytidylic acid copolymer (pi:pc) at -24, 0, or 24 h but not 72 h postinoculation (p.i.) of coxsackie-virus b3 (cvb3) to adolescent cd-1 mice. inoculation of interferon at any of the four times did not reduce virus titers in heart tissues at three or seven days p.i., but inoculation of pi:pc at -24, 0, or 24 h p.i. significantly ... | 1985 | 2985716 |
| hyperglycemia in balb/c mice after pretreatment with one subdiabetogenic dose of streptozotocin and subsequent infection with a coxsackie b4 strain. | male balb/c mice were injected with one subdiabetogenic dose of streptozotocin followed by coxsackie b4 virus infection 7 days later. the animals developed a transient hyperglycemia after streptozotocin-pretreatment and infection with a human coxsackie b4 isolate. frozen sections of pancreata stained with fitc-labeled antibodies showed an intensive infection of the exocrine tissue. immunofluorescence studies with isolated islets obtained from streptozotocin-treated or untreated animals demonstra ... | 1985 | 2986611 |
| systemic lymphoid atrophy in coxsackievirus b3-infected mice: effects of virus and immunopotentiating agents. | adult mice infected with coxsackievirus b3 (cb3) showed a generalized lymphoid involution. this effect was produced by most of the isolates and clones of cb3 and, to a lesser extent, by coxsackievirus b1 but not by other highly pathogenic picornaviruses. while moderate involution of the thymus also occurred in mild cb3 infections, peripheral changes strictly correlated with the severity of disease. the major alteration of the thymus was a massive cortical depletion, whereas the reduction of sple ... | 1985 | 2987368 |
| is insulin-dependent diabetes mellitus caused by coxsackievirus b infection? a review of the epidemiologic evidence. | the evidence that coxsackievirus b plays a causal role in the etiology of insulin-dependent diabetes mellitus (iddm) is reviewed. this hypothesis is biologically plausible; one variant of another picornavirus, encephalomyocarditis virus, causes diabetes in genetically susceptible mice, but prior infection with another, serologically indistinguishable variant prevents this. the seasonal distributions of infection due to coxsackievirus b and of iddm are similar. case reports document coxsackieviru ... | 1985 | 2988099 |
| [use of human embryonic fibroblasts for the laboratory examination of serious meningitis patients]. | 1985 | 2988213 | |
| serum antibodies against coxsackie b1-6 viruses in type 1 diabetics. | twenty-two newly diagnosed insulin-dependent diabetics and 46 control subjects have been examined for coxsackie b1-6 serum antibodies. evidence for coxb4 neutralizing antibodies at titers greater than or equal to 16 and two seroconversions for this single virus type were shown in the diabetic group (31.8% of patients with titers greater than or equal to 16 in comparison with 10% of controls, p = 0.08). reduced titers against coxb2 and coxb5 in diabetics were also observed. in agreement with the ... | 1985 | 2988253 |
| still's disease associated with coxsackie infection and haemophagocytic syndrome. | at the onset of still's disease (systemic-onset juvenile arthritis) in a 12-year-old girl, serological evidence of coxsackie b virus infection was found. two weeks later she developed a haemophagocytic syndrome which was then treated with cytotoxic therapy. her arthritis is still active six years later. | 1985 | 2988465 |
| cardiac involvement in coxsackie virus infection. | 1985 | 2989158 | |
| [dilated cardiomyopathy. an analysis of the viral-immunological theory]. | 1985 | 2990061 | |
| elimination of viruses and indicator bacteria at each step of treatment during preparation of drinking water at seven water treatment plants. | seven drinking water treatment plants were sampled twice a month for 12 months to evaluate the removal of indicator bacteria and cytopathogenic enteric viruses. samples were obtained at each level of treatment: raw water, postchlorination, postsedimentation, postfiltration, postozonation, and finished (tap) water. raw water quality was usually poor, with total coliform counts exceeding 105 to 106 cfu/liter and the average virus count in raw water of 3.3 most probable number of cytopathogenic uni ... | 1985 | 2990337 |
| method for selecting optimal cells for enterovirus isolation as determined in an outbreak of echovirus type 33 meningitis. | an outbreak of echovirus type 33-induced meningitis which occurred in belgium in 1982 is reported. to identify the causative agent, titers of an early isolate were measured on a variety of cells in order to select the optimal cells. a human rhabdomyosarcoma cell line was found to be the substrate of choice due to its proficient isolation of the virus. this method of determining infectious titres is recommended for improving enterovirus isolation in other epidemics. | 1985 | 2990920 |
| viral etiology of acute respiratory infections in south indian children. | 1985 | 2991137 | |
| purification of a hela cell receptor protein for group b coxsackieviruses. | coxsackievirus b3 (cb3) firmly attaches to hela cells, forming a specific complex between the virus and its receptor on the cell surface. we extracted this virus-receptor complex (vrc) with the detergents sodium deoxycholate and triton x-100. the vrc was identified by its sedimentation coefficient (140s), which was less than that of virions (155s). formation of the vrc from cell lysates and 3h-cb3 occurred with the same specificity as did attachment of virions to cells, in that its formation was ... | 1985 | 2991580 |
| steatitis in mice infected with coxsackie virus b3. | 1985 | 2991709 | |
| immunology of viral diabetes. | 1985 | 2992061 | |
| [pathology of organs of the urinary system in children with high risk of prenatal coxsackie virus infection]. | 1985 | 2992182 | |
| the postviral fatigue syndrome--an analysis of the findings in 50 cases. | the clinical, pathological, electrophysiological, immunological and virological abnormalities in 50 patients with the postviral fatigue syndrome are recorded. these findings confirm the organic nature of the disease. a metabolic disorder, caused by persistent virus infection and associated with defective immunoregulation, is suggested as the pathogenetic mechanism. | 1985 | 2993423 |
| electrophysiological studies in the post-viral fatigue syndrome. | single fibre electromyography (sfemg) was studied in 40 patients with the post-viral fatigue syndrome. these patients were also assessed clinically, serologically, virologically and immunologically. about 75% of the patients had definitely abnormal sfemg results. this was regarded as evidence of abnormality in the peripheral part of the motor unit. the muscle fibre was the likely site of involvement. | 1985 | 2993527 |
| [in vitro effectiveness of new antiviral substances. 2. studies with enteroviruses and rhinovirus lb]. | the antiviral efficacy of several compounds was demonstrated against coxsackie viruses type a9, b1, b3, b4 and b5, echoviruses type 6, 11, 30, and 33, attenuated polioviruses and rhinovirus 1b. in one-step growth cycle experiments virus multiplication was clearly inhibited by application of the compounds immediately after virus adsorption. | 1985 | 2994289 |
| an immunofluorescent study of generalized coxsackie virus b3 infection in a newborn infant. | an autopsy case of a 10-day-old newborn with generalized infection of coxsackie virus b3 (cbv3) was reported. cbv3 was isolated from the blood before death. the patient died of cardiac failure. an immunofluorescent study was carried out on autopsy specimens fixed in formalin and embedded in paraffin. cbv3 antigen was detected in the heart, brain, kidney, lungs, spleen, thymus, and pancreas. in the pancreas cbv3 antigen was predominantly seen in the islet cells. no cbv3 antigen was found in the l ... | 1985 | 2994361 |
| neutralization kinetic analysis of echovirus 30 and coxsackievirus b4 strains revealed little antigenic variation amongst the echovirus strains. | an antigenic analysis was made of echovirus 30 and coxsackievirus b4 isolates by determining neutralization rate constants in neutralization kinetic tests. the seven echovirus 30 isolates included the prototype strain and six others isolated in melbourne, australia, between 1959 and 1982. little antigenic heterogeneity was observed in contrast to the evidence of antigenic variation recorded in similar tests on seven coxsackievirus b4 isolates. these isolates also included the prototype strain, a ... | 1985 | 2994613 |
| hla-dr3 and -dr4 control t-lymphocyte responses to mumps and coxsackie b4 virus: studies on patients with type 1 (insulin-dependent) diabetes and healthy subjects. | to study the relationships between the responses to viral antigens and the hla-dr3 and -dr4 associations in type 1 (insulin-dependent) diabetes mellitus, the frequency of t-lymphocyte proliferating in response to mumps, coxsackie b4 and varicella-zoster antigens was determined. a decreased frequency was found in t lymphocytes able to respond to mumps or coxsackie b4 when presented together with dr3, as compared with the frequency of t lymphocytes able to respond to these viruses together with ot ... | 1985 | 2995183 |
| functional alterations in pancreatic beta cells as a factor in virus-induced hyperglycemia in mice. | alterations in the functional capacity of pancreatic beta cells appear to contribute to coxsackievirus b4-induced, long-term hyperglycemia in mice. mice infected with prototype b4 or its diabetogenic e2 variant were monitored for abnormalities in sugar metabolism (by the glucose tolerance test), for total protein and insulin synthesis in intact beta cells, for alterations in beta cell proteins, and for virus replication. the infected mice were hypoglycemic at 72 h postinfection and hyperglycemic ... | 1985 | 2995390 |
| a nonsteroid anti-inflammatory drug exacerbates coxsackie b3 murine myocarditis. | nonsteroid anti-inflammatory drugs are often used to treat myalgias and arthralgias in enteroviral infections, but their effects on acute viral myocarditis are unknown. the effect of the nonsteroidal anti-inflammatory drug, ibuprofen, on acute viral myocarditis was studied in 75 four week old male balb/c mice infected with 1.75 x 10(7) plaque-forming units of coxsackie virus b3 on day 0. ibuprofen was given intraperitoneally at a dose of 15 mg/kg body weight daily. the mice were assigned to four ... | 1985 | 2995470 |
| [humoral and cellular immunity in perimyocarditis and congestive cardiomyopathy]. | in coxsackie b-, influenza and mumps-induced perimyocarditis the characteristic immunological features were cross-reacting cytolytic antimyolemmal antibodies, circulating immune complexes and no nk- or k-cell activity. cytolytic antimyolemmal antibodies are markers of a secondary immunopathogenesis in perimyocarditis. in cytomegalovirus myocarditis antiinterfibrillary antibodies could be found and immune complexes were rarely observed. postmyocarditic cardiomyopathy could be identified by circul ... | 1985 | 2995721 |
| perspectives on the role of viruses in insulin-dependent diabetes. | insulin-dependent diabetes mellitus (iddm) results from the destruction of pancreatic beta cells. viruses have been suggested as one of the possible causes. the evidence for viruses comes largely from experiments in animals, but several studies in humans also point to viruses as a trigger of this disease in some cases. encephalomyocarditis (emc) virus, mengovirus (2t), and coxsackie b4 virus infect and destroy pancreatic beta cells when inoculated into mice. this results in hypoinsulinemia and h ... | 1985 | 2996865 |
| [sensitivity of group a coxsackieviruses and echo viruses, and isolation from clinical specimens by rd-18s cells derived by cloning from rd cells]. | 1985 | 2997341 | |
| group b coxsackieviruses readily establish persistent infections in human lymphoid cell lines. | exposing human lymphoid cell lines to uncloned or recently cloned group b coxsackieviruses results in the frequent establishment of chronically infected cultures. persistence is maintained by a carrier culture mechanism involving virus spread through the medium and replication among a minority of cells at any given time. these studies provide a model for persistence by highly cytocidal viruses. | 1985 | 2997486 |
| diabetogenic potential of coxsackie b viruses in nature. | thirty-seven clinical isolates of coxsackievirus (cv) serotypes b-1, b-3, b-4, and b-5 were inoculated into male sjl mice. twelve strains resulted in minor abnormalities of glucose metabolism in one or more of six infected mice (tables 1 and 2). sequential infection of male sjl mice with cvb-3, cvb-4, and cvb-5 resulted in abnormal glucose metabolism in 25 percent of the mice (fig. 1). the glucose index of the abnormal animals was similar to that produced by sequential infection with reovirus an ... | 1985 | 2998300 |
| hla heterogeneity of insulin-dependent diabetes mellitus at diagnosis. the pittsburgh iddm study. | although some previous studies have suggested that insulin-dependent diabetes mellitus (iddm) is a heterogeneous condition with variant forms being associated with hla-dr types, the evidence, thus far, is conflicting. to address this issue, we have examined the presenting characteristics of a consecutive admission series of 200 newly diagnosed cases of iddm from the children's hospital of pittsburgh. because hla-dr frequencies vary by race, data are presented only for the 172 white cases with co ... | 1985 | 2998911 |
| epidemic coxsackie b virus infection in johannesburg, south africa. | a particularly extensive epidemic of coxsackie b3 virus infection occurred in johannesburg in the spring and summer of 1984. a total of 142 positive cases were diagnosed by isolation of the virus from stools and other specimens (60) or by serology (82). coxsackie b3 accounted for 87% of the isolations and was also the dominant serotype on serology. the outbreak involved predominantly children and young adults, with no apparent sex differences being noted. the majority of specimens came from the ... | 1985 | 2999227 |
| cross-reactions of immunoglobulin m and g antibodies with enterovirus-specific viral structural proteins. | we analysed the reactivity of enterovirus-specific human igm and igg antibodies with the structural proteins of different enteroviruses by the immunoblot technique. in general, all immunoglobulin g antibodies of the tested sera reacted with capsid polypeptide vp 1 of the viruses tested (echoviruses 9 and 11, coxsackievirus b3 and poliovirus 2). in contrast, enterovirus specific immunoglobulin m antibodies of adults reacted with capsid polypeptides vp 1, vp 2, and/or vp 3 of the viruses mentioned ... | 1985 | 2999228 |
| high frequency of coxsackie-b-virus-specific igm in children developing type i diabetes during a period of high diabetes morbidity. | twenty-four consecutive children with newly diagnosed insulin-dependent (type i) diabetes mellitus (iddm) were investigated for a history of infectious disease. thirteen of the 24 (54%) patients reported symptoms of acute infection within two months before diabetes was diagnosed. the mean age was 8.5 years and 15 (63%) of the patients were girls. no clear seasonal variation in onset was seen. coxsackie b (cb)-virus-specific igm responses were detected by reverse radioimmunoassay (ria) in 16 of t ... | 1985 | 2999322 |
| persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83. | virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ... | 1985 | 3000066 |
| [ultrastructural alterations of rat myocardial cells in cell culture of the beating heart infected with coxsackie b-2 virus]. | 1985 | 3000541 | |
| cellular immune responses in mice challenged with an amyocarditic variant of coxsackievirus b3. | an amyocarditic variant of a temperature-sensitive (ts) mutant derived from the parent myocarditic variant coxsackievirus b3 (cvb3m) was studied in a murine model of cvb3m-induced myocarditis to assess virus-induced antigens and their possible role in the disease process. amyocarditic variant ts5r induced a heart tissue antigen(s), extractable by hypertonic kc1, which inhibited migration of peritoneal exudate cells from cvb3-inoculated myocarditic mice in an agarose droplet cell-migration-inhibi ... | 1985 | 3001222 |
| natural history of left ventricular function in neonatal coxsackie myocarditis. | three neonates are described who had severe congestive cardiac failure following coxsackie-b virus infection. overall left ventricular function was depressed and accompanied by regional differences in wall motion. recovery has been gradual, and after 40 or more months of follow-up, all three infants still have evidence of myocardial damage. this provides further evidence linking myocarditis with dilated cardiomyopathy. | 1985 | 3001658 |
| [viral myocarditis (the etiologic, clinical, diagnostic and treatment problems)]. | to elucidate the etiology of respiratory infection and pharyngitis associated myocarditis a serological study was made of 201 patients who were successively admitted with a clinical diagnosis of myocarditis. coxsackie viral infection of group b, influenza a and b, para-influenza and adenoviral infection and beta-hemolytical streptococcus of group a were determined. preceding coxsackie infection was established in 38,3% of the patients, influenza a and b in 27.5%, adenoviral infection in 3.6% and ... | 1985 | 3001963 |
| [effect of human leukocyte interferon on coxsackie b-2 virus-infected rat beating heart cells in culture]. | 1985 | 3002122 | |
| persistent infection of rat insulinoma cells with coxsackie b4 virus. brief report. | plaque purified coxsackie b4 virus (cb4) caused a persistent infection of rat insulinoma (rin) cells which lasted for the 70 day observation period. infectious virus was produced and no cytopathic effect was observed. indirect immunofluorescence and infectious center assays demonstrated that a majority of rin cells were infected. defective interfering particles were not demonstrated. despite persistent infection, insulin synthesis by rin cells was not altered. | 1986 | 3002301 |
| monoclonal antibody that inhibits infection of hela and rhabdomyosarcoma cells by selected enteroviruses through receptor blockade. | balb/c mice were immunized with hela cells, and their spleen cells were fused with myeloma cells to produce hybridomas. initial screening of culture fluids from 800 fusion products in a cell protection assay against coxsackievirus b3 (cb3) and the cb3-rd virus variant yielded five presumptive monoclonal antibodies with three specificities: protection against cb3 on hela, protection against cb3-rd on rhabdomyosarcoma (rd) cells, and protection against both viruses on the respective cells. only on ... | 1986 | 3003376 |
| detection by immunofluorescence of possible viral implications in "idiopathic" peripheral facial paralysis. | the presence of viral antigens was detected by the indirect immunofluorescence technique in exfoliated cells occurring in the pharyngeal exudate of 18 out of 29 patients with peripheral facial paralysis. the most frequently encountered antigens were: coxsackie a and b virus (33.3%), adenovirus (16.7%), and the association coxsackie b virus + adenovirus (16.7%). the possibility that some of the so-called "idiopathic" peripheral facial paralyses may have a viral etiology is discussed. | 1985 | 3004020 |
| [pathogenesis of virus-induced diabetes (a review of the literature)]. | 1985 | 3004041 | |
| coxsackievirus b-3 myocarditis. identification of different pathogenic mechanisms in dba/2 and balb/c mice. | dba/2 and balb/c mice, both h-2d, develop myocardial inflammation and necrosis when infected with a heart-adapted strain of coxsackievirus group b, type 3. similar inoculation of c57bl/6 (h-2b) animals results in minimal myocarditis despite equivalent heart virus titers in the three stains. thus, the host's genetic constitution influences the pathogenesis of the infection. anti-mouse thymocyte serum and monoclonal iad antibody effectively prevent myocarditis induction in dba/2 and balb/c mice, w ... | 1986 | 3004227 |
| concentration of viruses from water by using cellulose filters modified by in situ precipitation of ferric and aluminum hydroxides. | untreated cellulose filters adsorbed only small amounts of poliovirus 1, echovirus 5, coxsackievirus b5, or bacteriophage ms2 that were added to tap water or to solutions of imidazole-glycine buffer at ph 5 to 7. modification of filters by in situ flocculation of ferric and aluminum hydroxides greatly increased the ability of the filters to adsorb viruses. viruses adsorbed to the modified filters could be recovered by treating the filters with 3% beef extract (ph 9.5). greater than 60% of the en ... | 1985 | 3004332 |
| influence of inoculum size, incubation temperature, and cell culture density on virus detection in environmental samples. | the influence of inoculum size and cell culture density on virus titer by cytopathic effect or plaque assay was studied using poliovirus type 1 and bgm (buffalo green monkey) cells as a model for this evaluation. with a plaque assay system, a linear relationship was observed for an inoculum size of up 1 ml/25 cm2; a marked decrease in the number of plaques was observed when over 1 ml of sample was inoculated on this surface area. cell culture density also affected virus titer; maximal titers wer ... | 1985 | 3004683 |
| variations in the susceptibility to coxsackievirus b3-induced myocarditis among different strains of mice. | this study was undertaken to examine the inherent predisposition of different inbred strains of mice to develop coxsackievirus b3-induced myocarditis. a time course study established the pertinent, differential parameters of the disease and their corresponding genetic control. the a.by/snj (h-2b), a.sw/snj (h-2s), a.ca/snj (h-2f), b10.s/sgsf (h-2s), b10.pl/sgsf (h-2u), and c3h.nb/snj (h-2p) strains were found to vary widely in the extent and duration of viremia, in the temporal appearance and ti ... | 1986 | 3005402 |
| coxsackie b virus-specific igm responses in coronary care unit patients. | an enzyme-linked immunosorbent assay (elisa) test using polyvalent antigens and antisera was used to detect coxsackie b virus-specific igm responses in 329 patients admitted to the coronary care unit, wellington hospital, new zealand over a 12-month period. the sera of 30 of 153 (19.6%) patients with acute myocardial infarction (ami), 16 of 98 (18.4%) with chest pain, and 7 of 46 (15.2%) patients with arrhythmia were positive for coxsackie b virus-specific igm. four of 12 (25%) patients with hea ... | 1986 | 3005490 |
| [comparison of serous meningitis morbidity and the concentration of the coxsackie b virus in sewage]. | the paper shows that the frequency of detection and concentration of coxsackie b virus in sewage correlate with the number of patients and asymptomatic virus excretors. | 1986 | 3008437 |
| severe pancreatitis and fatty liver progressing to cirrhosis associated with coxsackie b4 infection in a three year old with alpha-1-antitrypsin deficiency. | a 3-year-old girl in whom severe acute pancreatitis was associated with evidence of coxsackie b4 virus infection was alpha-1-antitrypsin deficient. lack of this modulator of proteolysis may have been responsible for her severe course. fatty liver at presentation progressed to cirrhosis in the ensuing 18 months. | 1986 | 3008494 |