Publications

[acute transverse myelitis associated with echo-25 virus infection].	a case of acute transverse myelitis associated with echo-25 virus infection was reported here with a brief review of the literature. the patient was a 42-year-old house wife. without any antecedent symptoms, weakness and paresthesia of bilateral lower extremities, and sphincter disturbance developed suddenly. neurological examination revealed paraplegia of both legs, hyperreflexia of lower extremities, bilateral positive babinski sign and impairment of all sensory modalities below th4 level. no ...	1990	2242635
[the geographical spread of enterovirus uveitis in the ussr].	enteroviral uveitis is accompanied by intensive and long-term production of specific neutralizing antibodies. the antibody titer to the causative virus echo 19/k (siberia, krasnoyarsk, 1980-1981) was 1:1000-1:65,000 in 80% of children 5 to 6 1/2 years after the illness. in retrospective examinations of blood sera from children with the history of uveitis using a neutralization test with ophthalmotropic strains of echo 19 and echo 11 viruses, the echo-virus etiology of this disease was first esta ...	1990	2256316
interferon-sensitive coxsackievirus variants in nature.	variants of a clinical isolate of coxsackie b-4 virus that differ in interferon (ifn) sensitivity were selected from virus stock both before and after passage in mice. ifn sensitivity was a stable property on subsequent plaque selection. the ifn-sensitive variant was less virulent than its ifn-insensitive counterpart, whether from the original virus stock or from mouse passaged virus. none of these variants was diabetogenic in mice. these studies demonstrate that coxsackievirus variants of diffe ...	1985	2409191
t lymphocyte responses to coxsackie b4 and mumps virus. ii. immunoregulation by hla-dr3 and -dr4 associated restriction elements.	a major part of the t lymphocyte response to mumps and coxsackie b4 virus appears to be restricted by hla-dr associated restriction elements. this was further corroborated in inhibition experiments using monoclonal antibodies reactive with different hla class ii molecules. only antibodies reactive with dr molecules significantly inhibited the response. the frequencies of dr restricted antigen-reactive t lymphocytes (artl) to mumps and coxsackie b4 virus were then investigated, using a limiting d ...	1985	2413585
characterization of an echovirus type 30 variant isolated from patients with aseptic meningitis.	an outbreak of echovirus type 30 infection associated with aseptic meningitis occurred among newborn babies in a hospital neonatal room at fukui city in 1983. the isolated virus was identified as an antigenic variant of echovirus type 30 by cross-neutralization tests with antisera against the prototype bastianni strain and the present isolate. western blot analysis demonstrated that the antigenic determinants responsible for virus neutralization, some of which were type specific and others strai ...	1986	2425228
humoral immunity against viral antigens in type 1 diabetes: altered iga-class immune response against coxsackie b4 virus.	a group of 210 pediatric type 1 diabetic patients with long duration of illness and their matched controls (age range 2-19 years) were analysed for coxsackie b4 antibodies in igg-, igm- and iga-antibody classes by enzyme-linked immunosorbent assay (elisa). about 60% of both patients and controls were seropositive. however, patients had higher prevalence and mean levels of iga-class antibodies compared to controls. no such difference was found in igg- or igm-antibody classes. the elevation of iga ...	1986	2426921
antigen-specific suppressor t cells prevent cardiac injury in balb/c mice infected with a nonmyocarditic variant of coxsackievirus group b, type 3.	two variants of coxsackievirus group b, type 3 (cvb3o and cvb3m) infect balb/c cardiac tissue equally, but only one variant (cvb3m) induces myocarditis. various studies indicate that disease resistance in cvb3o-infected mice results from generation of suppressor cells. low-dose cyclophosphamide (50 mg/kg body weight) treatment enhances inflammation in both cvb3m and cvb3o-infected animals, which clearly demonstrates that cvb3o has the capacity to induce disease, but inhibitory factors must exist ...	1986	2432792
coxsackievirus b-3-induced myocarditis. virus and actinomycin d treatment of myocytes induces novel antigens recognized by cytolytic t lymphocytes.	balb/c mice inoculated with 6 x 10(4) plaque-forming units of a myocarditic variant of coxsackievirus, group b, type 3 (cvb3m) developed three distinct ctl populations recognizing putative virion (virus specific ctl, vctl), normally expressed heart (autoreactive ctl, actl), and aberrant metabolic (mctl) ag. induction of the mctl-specific ag on cardiocytes correlated with the ability of the myocarditic viruses and actinomycin d to interfere with cellular metabolism as measured by 3h-uridine and 3 ...	1988	2459248
intracellular detection of sense and antisense enteroviral rna by in situ hybridization.	we prepared sense (s) and anti-sense (as) 3h-labelled single-stranded rna probes by polymerase-directed in vitro transcription of a coxsackievirus b3-derived cdna fragment cloned in the pgem2 plasmid vector. the probes detected, by in situ hybridization, both s and as forms of viral rna in the cytoplasm of coxsackievirus-infected tissue culture cells. more s (genomic and messenger) rna was present than as (negative and replicative intermediate) rna. we confirmed and quantitated this observation ...	1988	2464608
murine cell-mediated immune response recognizes an enterovirus group-specific antigen(s).	splenocytes taken from mice inoculated with coxsackievirus b3 (cvb3) (nancy) developed an in vitro proliferative response against cvb3 antigen. this response could not be detected earlier than 8 days postinoculation but could be detected up to 28 days after exposure to cb3. cvb3-sensitized splenocytes responded not only to the cvb3 antigen but to other enteroviruses as well. this response was found to be enterovirus specific in that no response was detected to a non-enteroviral picornavirus, enc ...	1989	2476566
presence of an anti-viral factor in peritoneal dialysis effluent.	viral peritonitis is an exceptionally rare occurrence in peritoneal dialysis. in fact, up to now, only one case report has been documented in the literature. in a prospective study, peritoneal dialysis effluent (pde) was specifically cultured for the following viruses: the herpes group of viruses, including herpes simplex types i (hsv) and ii, cytomegalovirus (cmv) and varicella-zoster (v-z), and the enteroviruses group including coxsackie b-5 (cox b), echo, enterovirus and polio. cultures were ...	1989	2476580
features of coxsackie b virus (cbv) infection in children with prolonged physical and psychological morbidity.	a retrospective study of the clinical features in 39 children who were investigated for evidence of coxsackie b virus (cbv) infection is reported. eighteen children were found to have serological evidence of infection. an extensive range of features was elicited in both seropositive and seronegative patients, most children complaining of abnormalities referable to muscle and, in particular, of weakness and easy fatiguability. children with evidence of cbv infection were significantly more likely ...	1989	2538617
use of betapropiolactone to disinfect fresh tissue without impairing antigenicity: method applicable to human immunodeficiency virus (hiv) positive tissue.	a method for inactivating viruses in tissues is reported that does not impair the antigenicity of the coxsackie virus or of some common tissue antigens, a common problem with standard tissue fixation methods. tissues can be placed briefly in betapropiolactone before cryostat sectioning without any adverse effect on preservation or antigen expression. it is suggested that use of betapropiolactone is applicable to tissues harbouring or exposed to the human immunodeficiency virus (hiv). as betaprop ...	1989	2539401
chronic neonatal coxsackie myocarditis.	a baby girl, with a birth weight of 2540 g, developed myocarditis with gross, permanent myocardial calcification. the clinical course was progressively downhill, and she died at 14 months of age. neutralisation antibody to coxsackie virus group b1 rose from 1:80 after delivery to 1:1280 at age 5 months. the protracted course of this infant's disease represents a new clinical form of fatal neonatal coxsackie virus group b infection.	1989	2539789
effects of the immunomodulator ls 2616 on lymphocyte subpopulations in murine coxsackievirus b3 myocarditis.	quinoline-3-carboxamide (ls 2616) is a broadly acting immunostimulator with anti-inflammatory effects in coxsackie virus b3-induced myocarditis in female balb/c mice. this infection caused extensive inflammatory and necrotic lesions in the myocardium 7 days after inoculation (6.8% of tissue section area). the damaged area was reduced (to 3.7% (p less than 0.05] and the lethality decreased when ls 2616 was administered over 14 days, starting 7 days before the inoculation. the response pattern of ...	1989	2540239
cell-mediated immune cardiocyte injury in viral myocarditis of mice and patients.	the cellular immune mechanism of cardiocyte injury in viral myocarditis was investigated by observing and analyzing the interactions among cardiocytes, t cells, b cells, natural killer (nk) cells and macrophages in situ in the myocardium of our murine model (c3h/he mice) and of human patients. in murine coxsackie b3 virus myocarditis, lymphocyte subsets were identified by light and electron microscopic immunohistochemical techniques with antibodies against specific antigens of pan t (thy 1.2), h ...	1989	2541265
laboratory diagnosis of viral myocarditis. a review.	this review delineates the impact of molecular biology on the diagnosis and pathogenesis of coxsackie b viral myocarditis. the etiological role of the coxsackie b viruses in acute myocarditis is well established. a hallmark of most coxsackie b virus infections is extension cytopathology of the host cell. recent evidence from studies of a nonlytic infection show that a coxsackie b virus can alter an infected cell which results from persistent infection. there are two other mechanisms for cardiac ...	1989	2541266
cytoplasmic islet cell antibodies in children with type i diabetes.	in a group of 72 type i juvenile diabetics we investigated the prevalence of islet cell antibodies (ica). their occurrence rate was followed up over 1-3 years. in 66 patients also neutralizing antibodies and igm antibodies to coxsackie b viruses were determined. ica positivity was recorded in 21 out of 38 coxsackie positive diabetics (55%), compared to 13 out of 28 coxsackie negative diabetics (46%). ica were found in a total of 73% of diabetic patients. the postinitial course of diabetes, asses ...	1989	2541873
monoclonal antibody therapy for prevention of acute coxsackievirus b3 myocarditis in mice.	the efficacy of monoclonal antibodies against t cell subsets in the therapy of experimental myocarditis caused by coxsackievirus b3 (cb3) was investigated. two-week-old male c3h/he mice were inoculated with cb3 virus. treatment was begun in the viremic stage (starting on the day of inoculation) in experiment 1 and in the later aviremic stage (starting on day 10) in experiment 2. rat anti-mouse monoclonal antibodies, lyt 1 (helper/inducer t) at 1 microgram/mouse (group 2 in experiment 1; group 6 ...	1989	2541943
in vivo infection of mice with coxsackie b4 virus induces long-term functional changes in pancreatic islets with minimal alteration in blood glucose.	the long-term effects of coxsackie b4 (cb4) infection of mice on pancreatic islet function were investigated. mice were inoculated with various strains of cb4 virus and 2, 3 and 6 months later islet insulin synthesis and release from isolated islets were measured. insulin release at basal glucose concentration (2 mmol l-1) was higher in islets from mice inoculated with pancreas-adapted cb4 strains than in control islets or those from mice inoculated with tissue culture-adapted cb4. thus, two str ...	1989	2541964
exercise in coxsackie b3 myocarditis: effects on heart lymphocyte subpopulations and the inflammatory reaction.	to investigate whether exercise in coxsackie b3 virus infection is detrimental to the myocardium, balb/c mice were inoculated with the virus and exercised to exhaustion on a motor-driven treadmill up to 48 hours after the inoculation. this infection evokes myocarditis. the inflammatory and necrotic lesions in the ventricular myocardium 7 days after the inoculation covered 4.32% of the tissue section area in the nonexercised group. exercise at 0 hours did not affect this myocardial damage (4.77%) ...	1989	2543197
coxsackie b virus assays in iddm families: the gaw5 data on antibody prevalence.	there is evidence that infection by coxsackie viruses can serve as an environmental "trigger" for insulin-dependent diabetes mellitus (iddm). this background information is summarized briefly, and the data in the iddm component of genetic analysis workshop 5 (gaw5) concerning serum antibodies to coxsackie b viruses are described.	1989	2543606
[the role of prenatal coxsackievirus infection in the etiology of congenital heart defects in children].	sixty-two children with congenital heart diseases and 100 normal children were examined, their age ranging from 5 days to 6 years. indicators of intrauterine enterovirus infection risk were detected in 93.5% of children with congenital heart diseases and in just 21.9% of the controls. coxsackie a and b viruses were detected by immunofluorescence in urinary sediment cells of 84.4% of children with congenital heart diseases vs. 7.7% of the controls. none of the 20 serotypes of coxsackie a virus wa ...	1989	2543861
coxsackie b3 virus encephalitis in a patient with agammaglobulinaemia.	a 19-month-old boy with agammaglobulinaemia contracted a coxsackie b3 virus infection which caused meningoencephalitis. his neurological status deteriorated over the following 4 years and he died aged 6 years. treatment with gammaglobulin i.m. and igg-fab2 fragments i.v. was not effective. enterovirus can cause life-threatening infections in immunodeficient patients.	1989	2545452
coxsackie virus and urogenital pathology.	in 43 women (average age 49.6 years), and 70 men (average age 55.2 years) with pathology of the genito-urinary apparatus, seroantibodies to coxsachie virus b were measured using the passive hemoagglutination method and the virus was isolated in the uterine. viral isolation test was negative in all urine samples tested. seropositivity for coxsackie virus was reported in 26 women (60.46%) and in 51 men (72.85%). positivity to b1 was 37.16% (42 cases), b2 in 38.05% (43 cases), and to b4 in 35.39% ( ...	1989	2547535
monoclonal anti-idiotypic antibodies regulate the expression of virus-induced murine myocarditis.	a monoclonal anti-idiotypic antibody (anti-id), produced by electrofusion and designated anti-id88, was able to modulate expression of murine autoimmune myocarditis mediated by coxsackievirus b3 (cvb3). the anti-id was characterized as an immunoglobulin g2b species possessing kappa light chains and was able to reduce expression of inflammatory myocarditis in anti-id-pretreated mice challenged with cvb3. anti-id88 was able to stimulate specific cell-mediated immunity against anti-id88, as well as ...	1989	2547722
factors affecting virus plaque confirmation procedures.	direct plaque counts obtained by using the monolayer cell culture assay technique reliably confirmed the number of viruses isolated. analysis revealed some significant differences in the false-positive rate, depending on the test method used or virus samples evaluated. plaques from laboratory stock viruses showed a higher confirmation rate than sewage plaque isolates. test results with laboratory stock viruses suggested that confirmation rates may be affected by virus types present in the sample ...	1989	2547809
enterovirus igm detection: specificity of mu-antibody-capture radioimmunoassays using virions and procapsids of coxsackie b virus.	a predominantly type-specific mu-capture radioimmunoassay (ria) of igm antibodies to coxsackie b1-b5 (cb1-cb5) viruses was previously described (frisk et al., 1984). the present study is concerned with the specificity of this assay, using as antigen different strains of one serotype (cb5) and procapsids of two serotypes (cb3 and cb5). eight strains of cb5 virions were tested against acute and/or convalescent sera from 10 patients from whom cb5 had been isolated. seven patients' sera were tested ...	1989	2547815
coxsackievirus b3-induced acute pancreatitis: analysis of histopathological and viral parameters in a mouse model.	coxsackievirus b3 infection in mice was studied histopathologically, by virus isolation and by nucleic acid hybridization after intraperitoneal inoculation of the virus. extensive viraemia was detected for 1-3 days post-infection. all mice developed necrotizing acute pancreatitis and focal myocarditis. pancreatitis eventually lead to complete atrophy of the exocrine pancreas. however, the islets of langerhans and pancreatic ducts remained morphologically intact. virus could be demonstrated in pa ...	1989	2548562
use of synthetic oligodeoxyribonucleotides for type-specific identification of coxsackie b viruses.	synthetic oligodeoxyribonucleotides were used for type-specific identification of members of the coxsackie b virus group by nucleic acid hybridization. two pairs of oligonucleotide chains were constructed based on nucleotide sequences in the vp1 regions of coxsackieviruses b3 and b4. each labelled probe had a length of 24 nucleotides. the results showed that the oligonucleotide hybridized in a type-specific manner when assayed with extracts from cells infected with all different coxsackie b viru ...	1989	2549405
[the morphology of coxsackie b virus infections of the nervous system].	neuropathological studies were conducted into 20 virologically secured coxsackie-b virus infections of infants and adults, with the nervous system being involved. inflammatory processes affect both gray and white cerebral regions as well as the peripheral nervous system, and in adulthood they may take the form of severe necrotising encephalitis. necroses of that kind may be recordable from various regions of the central nervous system. notwithstanding tempestuous progress in the development of m ...	1989	2549750
[effect of astragalus membranaceus on electrical activities of coxsackie b-2 virus-infected rat myocardial cells in culture].	beating myocardial cell cultures of neonatal rats were prepared in vitro and infected with coxsackie b-2 virus. the cells were evaluated in the post-infected period for changes in beating percentage and cytopathic effect (cpe), alterations in the electrical activities by standard microelectrode techniques, and the protective effect of astragalus membranaceus (am) on coxsackie b-2 virus-infected neonatal rat myocardial cell cultures was observed. the beating percentage began to decrease in the in ...	1989	2550160
enteroviral-related antigen in circulating immune complexes of amyotrophic lateral sclerosis patients.	circulating immune complexes were isolated by polyethylene glycol precipitation from the sera of patients with amyotrophic lateral sclerosis. rabbits immunized with circulating immune complexes from 3 of 5 amyotrophic lateral sclerosis patients induced antisera that specifically reacted with enterovirus-infected cells by immunofluorescence and enzyme-linked immunosorbent assay. these antisera were nonneutralizing and did not react with purified virus. in addition, peripheral lymphocytes of amyot ...	1989	2550387
coxsackievirus-b3-induced myocarditis: virus receptor antibodies modulate myocarditis.	two variants of coxsackievirus group b, type 3 (cvb3) differ in ability to induce myocarditis in balb/ccum mice. infection with the highly pathogenic variant (cvb3m) stimulates autoimmunity to normal cardiocyte antigens, and tissue injury results primarily from an autoreactive cytolytic t lymphocyte (actl). animals infected with the less pathogenic cvb3o variant do not develop actl, although cvb3o replicates to high titers in the heart and polyclonal neutralizing antisera fail to distinguish bet ...	1989	2550544
fatal coxsackie b1 virus infection in neonates.		1989	2552390
[macrophage disappearance reaction in mice during an infection caused by coxsackie b3 virus].	intraperitoneal inoculation of 2 1/2-3-month-old male balb/c mice with coxsackie b3 virus in a dose of 10(7.2) tcd50 was demonstrated to be accompanied by inhibition of the starch-induced exudation of leukocytes into the peritoneal cavity. a decrease in the number of macrophages was observed in 3-day exudates induced at 4 and 21 days but not at 0, 7, and 14 days of infection. the macrophage disappearance reaction showed that the intraperitoneal inoculation of the challenge virus dose (10(7.2) tc ...	1989	2552686
end-stage dilated cardiomyopathy. persistence of enterovirus rna in myocardium at cardiac transplantation and lack of immune response.	tissue from the explanted hearts of 21 patients with idiopathic dilated cardiomyopathy or 19 patients with other specific heart muscle diseases were investigated for presence of enterovirus-specific rna with an enterovirus group-specific cdna probe. this was complementary to coxsackievirus b2 rna sequences between nucleotide numbers 6,550 and 7,400, which are highly conserved between enteroviruses. hearts from six patients with dilated cardiomyopathy and one patient with ischemic heart disease w ...	1989	2553297
intrathecal synthesis of virus-specific oligoclonal antibodies in patients with enterovirus infection of the central nervous system.	cerebrospinal fluid (csf) and serum samples from six patients with enterovirus infections were investigated by isoelectric focusing (ief) and affinity-mediated immunoblot ami) for the clonal distribution of entervirus-specific antibodies. in two patients with either acute meningitis or encephalitis and in one patient with a relapse of multiple sclerosis, oligoclonal igg bands specific for enteroviruses were found predominantly in the csf, revealing intrathecal synthesis of these antibodies. in t ...	1989	2553877
the swedish childhood diabetes study iii: igm against coxsackie b viruses in newly diagnosed type 1 (insulin-dependent) diabetic children--no evidence of increased antibody frequency.	sera from essentially all swedish children aged 0-14 years with type 1 (insulin-dependent) diabetes mellitus with onset during an autumn period (october-december 1985) and a late spring period (may-june 1986) were selected. in all, 98 patients were analysed for igm antibodies against coxsackie b virus serotypes 1 through 5 by a mu-antibody capture radio immunoassay technique. sera from 94 referent children matched for age, sex and residential area, collected during the same period, were also ana ...	1989	2556308
enterovirus-specific igm in the diagnosis of meningitis.	samples of serum from 557 patients with a clinical diagnosis of meningitis or encephalitis and referred to the epsom public health laboratory during a period of 3 years were tested for enterovirus-specific igm in a mu capture enzyme-linked immunosorbent assay (elisa). enterovirus-specific igm was detected in 45% samples from all age groups. in the 3-5-year age group, 67% specimens were positive. a notable male predominance (73%) was seen in the age group 0-15 years. as predicted, a seasonal incr ...	1989	2557369
chronic coxsackie b infection mimicking primary fibromyalgia.	we describe a patient with primary fibromyalgia according to smythe's criteria who had evidence of chronic infection with coxsackie virus over a 4-year period. we discuss the association of coxsackie infection and myalgic syndromes and review the literature.	1989	2557447
[the hemagglutinating properties and adsorption on erythrocytes of coxsackie b-3 viruses and their selected bentonite variants].	the paper is devoted to the study of the coxsackie b-3 viruses and their bentonite variants passaged on the primary and transplanted cell cultures for their hemagglutinating (ha) properties and a degree of adsorption on erythrocytes. it is shown possible to detect the ha activity of the coxsackie b-3 viruses which are passaged on the transplanted cell cultures: the variant abent has the hemagglutinating activity relative to human and rabbit erythrocytes. the variant abent+ of the coxsackie b-3 v ...	1989	2558279
[study of the antiviral activity of nonsteroidal anti-inflammatory agents in cell culture].		1989	2558448
analysis of virus protein heterogeneity among group b coxsackie viruses using a "mini" two-dimensional gel electrophoresis system.	the application of a small format two-dimensional polyacrylamide gel electrophoresis (2d-page) system to the study of protein heterogeneity among group b coxsackie virus (cvb) isolates is described. under the conditions of electrophoresis developed during this study, protein samples could be processed within 7 h and up to 300 intracellular proteins were resolved from uninfected hep-2 cell lysates. 2d-page was used to characterise the intracellular proteins of clinical cvb isolates of serotypes 4 ...	1989	2558885
[dilated cardiomyopathy: sequela of viral myocarditis?].	the study was undertaken to examine 100 patients with dilated cardiomyopathy (dcm) and 62 with viral myocarditis (vm). serological studies revealed that 60% of the vm patients and 25% of the dcm patients had diagnostically significant antibody titers to coxsackie b virus. the patients from the two groups displayed nk cell deficiency, quantitative and functional suppressor defect, and severe derangements in the system of interferons. unlike dcm patients, some vm patients showed greater quantities ...	1989	2559232
pig erythrocyte ghost cells used for concentration of enteric viruses from experimentally contaminated clinical specimens.	a simple, rapid and efficient procedure of virus concentration from urine, cerebrospinal fluid (csf), and feces was developed. pig erythrocyte ghost cells were used to adsorb and elute such viruses as poliovirus lsc-1, echovirus 6, and human rotavirus (clinical isolate). in urine and csf, the adsorption efficiency range was 80-100% and elution was from 85% to greater than 100%. in addition, poliovirus lsc-1 was used as an experimental model to examine this procedure under various clinical condit ...	1989	2559952
anti-mouse igm immunoglobulin protects weanling mice from coxsackievirus infection.	mice treated from birth with rabbit anti-mouse igm antiserum (anti-mu), although immunosuppressed in regard to b cell function, may paradoxically be protected when challenged with certain viruses. our early studies indicated that short term treatment of weanling mice protected against semliki forest virus, herpes simplex virus, and coxsackievirus b1. for this study, the murine model of coxsackievirus b1 infection was chosen to determine if specific anti-mu immunoglobulin (anti-mu ig) would prote ...	1989	2560114
severity of disease induced by a pancreatropic coxsackie b4 virus correlates with the h-2kq locus of the major histocompatibility complex.	coxsackie b viruses are known etiological agents of pancreatic diseases, including diabetes. the pathogenesis of these infections is influenced by both host and viral factors. in this report, we examined whether the outcome of coxsackie b4 virus infection is dependent on the genes within the major histocompatibility complex (mhc). we generated a pancreatic variant, cb4-v and established an animal model system of pancreatitis with concurrent hypoglycemia in mice. infection of various b10 h-2 cong ...	1989	2560295
[effect of dexamethasone on coxsackie b-2 virus-infected rat beating heart cells in culture].	the effects of dexamethasone (dex) on cultured rat beating heart cells infected with 100 tcid-50 coxsackie virus b-2 (cb2v) were observed. the beating % began to decrease in the infected group 2 or 3 d post-challenge. meanwhile, the cytopathic effect (cpe) appeared rapidly from 1+ to 3+. in the infected and dex-treated group 1 h after inoculation, the beating % and cpe in the whole flask were significantly higher and less, respectively, than that in the group infected (p less than 0.05) at the s ...	1989	2560313
viral contamination of some drinking waters in romania: a twenty years survey.	a synthesis is made on the 20 years virological survey of the drinking water from some towns of romania. the sampling of the water was made by the gauze-pad method. the virus concentration method by adsorption-elution with the yeast cells was applied concomitantly, at first with the pe60 method, then, during the last years, either with aluminum hydroxide or with the polymer pv methods, and the concentrates were inoculated both into suckling mice and into cell cultures. various types of coxsackie ...	1989	2560479
coxsackie virus and rheumatic fever. a correlative study.	twenty-one patients of whom 13 had acute rheumatic fever and 8 had recurrence of rheumatic fever were studied for the evidence of coxsackie b viral infection using neutralisation test. a significant rise was noted in 17 cases (81%) and two cases had very high initial titre of neutralising antibodies to coxsackie b viruses type 1 to 6. mixed infection with more than one serotype was seen in 11 cases. coxsackie b2 was the commonest type and 14 patients had antistreptolysin 'o' anti-bodies. the hig ...	1989	2561125
[the role of coxsackie b viruses in the etiology of dilated cardiomyopathy].	in order to establish the relationship between coxsackie b virus infection and dilated cardiomyopathy (dcmp), 53 patients suffering from dcmp underwent serological tests. the rate of demonstration of virus-neutralizing antibodies at different titres was compared with the respective values in the previously examined 180 patients with myocarditis, 58 patients with myocardial sclerosis and 150 normal persons. in dcmp, antibodies against b2 type virus were detectable more frequently (p less than 0.0 ...	1989	2561232
low levels of mumps virus antigen induced interferon-alpha production in insulin-dependent diabetes.	the in vitro production of interferon-alpha was studied in cultures of peripheral blood mononuclear cells from children with insulin-dependent diabetes. significantly lower levels (p less than 0.01) of interferon (median 64 iu/ml) were found in mumps antigen stimulated cultures of iddm patients compared to control children (median 256 iu/ml) whereas no differences between groups were found in the amount of interferon induced by coxsackie b pool antigen or live sendai virus.	1989	2561352
[determination of antibody levels against coxsackie b virus type 2 and type 3 in sera from blood donors using the microneutralization test].	the presence of antibodies and their titers against coxsackie b virus type 2 and type 3 (cbv-2 and cbv-3) have been investigated in 208 blood donor's sera obtained from blood bank of gülhane military medical academy. the seropositivity against coxsackie b virus type 2 and type 3 have been determined 59.1% (123/208) and 7.2% (15/208) respectively. these findings suggest that coxsackie b virus type 2 infections may be present more widely than coxsackie b virus type 3 infections in our country.	1989	2562136
chronic relapsing pericarditis and dilated cardiomyopathy: serological evidence of persistent enterovirus infection.	by means of two different igm-capture assays, enterovirus-specific igm responses were shown in 9 of 14 (64%) patients with chronic relapsing pericarditis. this finding suggests persistent enterovirus infection, particularly coxsackie b virus infection. igm responses persisted for at least 1 year and for up to 10 years after onset of symptoms. in contrast, patients with acute enterovirus infections, including acute pericarditis, had transient responses. among patients with acute pericarditis, the ...	1989	2564902
coxsackievirus-induced disease. cd4+ cells initiate both myocarditis and pancreatitis in dba/2 mice.	dba/2 male mice inoculated intraperitoneally with 1.8 x 10(5) plaque-forming units (pfu) coxsackievirus b-3 (cvb3) showed extensive inflammatory cell infiltration of the myocardium and acinar tissue of the pancreas in 7 days. selective depletion of t lymphocyte subpopulations indicated that cd4 cells were either completely or partially responsible for cell damage in both organs. other organs such as the liver were infected and contained virus titers equivalent to those seen in the heart and panc ...	1989	2573284
antimyocarditic activity of the guanine derivative biolf-70 in a coxsackievirus b3 murine model.	prophylactic administration of a nontoxic dose of 9-[[2-benzyloxyl-1-(benzyloxymethyl)ethoxy]methyl]-6-chlo roguanine (biolf-70) to mice reduced the number of myocarditic lesions induced by coxsackievirus b3 (cvb3). biolf-70 exhibited minimal antiviral activity against cvb3 in hela cells and murine neonatal skin fibroblasts and minimally reduced cvb3 yields in heart tissues. the drug had no effect on serum anti-cvb3 neutralizing antibody titers and did not induce the production of interferon. fl ...	1985	2580480
steady-state infection by echovirus 6 associated with nonlytic viral rna and an unprocessed capsid polypeptide.	we established a human cell line which was persistently infected (pi) by the normally cytolytic echovirus 6. all of the cultured pi cells contained genome-size viral rna which was synthesized continuously and incorporated into virus particles. this steady-state infection has been maintained for more than 6 years. in contrast to rna of wild-type echovirus 6, the viral rna from pi cells was not lytic when transfected into uninfected, susceptible cells. the capsid polypeptides of the virus particle ...	1989	2585604
fatal outcome of echovirus 7 infection.	a 9-month-old boy died of a disseminated echovirus 7 (e7) infection with virus being cultured from his liver and spleen. his hepatic failure was complicated by candidiasis and marrow hypoplasia and aggravated by a possible immune deficiency.	1989	2587948
severe encephalitis associated with disseminated echovirus 22 infection.	severe encephalitis associated with disseminated echovirus 22 infection occurred in a previously healthy 5-month-old boy. echovirus 22 was diagnosed by a seroconversion both in serum and cerebrospinal fluid and by isolation of the virus from several stool samples. the child damaged severely and at the age of 8 1/2 months infantile spasms developed.	1989	2587949
[non-poliomyelitis viruses in poland 1982-1987].	diseases caused by non-polio enteroviruses (enp) are diagnosed virologically and serologically in 17 virology laboratories of the province sanitary-epidemiological stations and the department of virology, state institute of hygiene. the results of these investigations are available in the form of documentation including the type of the isolated virus, the result of serological investigation, the date of disease onset, the clinical diagnosis, the age and sex of the patient. these data were analyz ...	1989	2616797
[role of viral reactivation in recurrent adenoiditis and tonsillitis in children].	we have been made virus cultures, excepting ebv, in adenoid and palatine tonsil tissue come from adenotonsillectomy. they were practised to eighteen patients between 4 and 17 years old who suffered recurrent infections processes of these organs. the total was 22 specimens to estimate the reactivation function of latent virus in the etiology of this pathology.	1989	2629934
[echo-ii virus, the pathogen of the third outbreak of uveitis in children in kransnoyarsk, 1986].	the third outbreak of an acute infection accompanied in 3 cases by inflammation of the uveal membrane (uveitis) was recorded among infants in krasnoyarsk in 1986. from the infants with the eye involvement as well as from those in contact with the former, 36 strains of echo-11 virus were isolated, and seroconversion to this virus was demonstrated. all the infants in contact with the eye patients were given gamma-globulin which seemed to limit the spread of the infection. the third outbreak of uve ...	1989	2728407
[immunomodulation mechanisms in the anti-tumor effect of the echo-7 enterovirus].	it has been established that the complex systemic and local administration of human echo-7 virus exerts an inhibitory effect on the growth of mx-17 tumour in the balb/c mice without the signs of viral oncolysis. the antitumour effect is due to a modulation of the antitumour immunity (immunological regression) against a background of the viral immunogenesis.	1989	2791955
[morphofunctional changes in a culture of pancreatic islet cells from newborn piglets as affected by coxsackie b3 viruses].	the direct influence of coxsackie virus b-3 and its selected variants abent+ and abent- on the function and structure of neonatal pig pancreatic islet cells in monolayer cultures was studied. the viruses under study possessed cytopathogenic activity and were capable of reproduction. the abent- variant of coxsackie virus b-3 possessed the most pronounced cytopathogenic activity. direct correlation between virus reproduction and insulin concentration in a culture medium was established.	1987	2821534
evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease.	we previously noted a region of amino acid sequence homology between a-gliadin, a major alpha-gliadin component known to activate coeliac disease, and the early region e1b protein of human adenovirus serotype 12 (ad12), an adenovirus isolated from the human intestinal tract. in the present study sera from coeliac disease patients from the united kingdom and the united states were assayed for neutralising antibody to ad12 as evidence of past exposure to that virus and for antibody to synthetic pe ...	1987	2822550
the histopathology of enterovirus infections of new-born mice.	for 32 years we have provided a routine histopathological service on animals for the regional virus laboratory, ruchill hospital, glasgow. this article concerns mainly experimental coxsackievirus a, coxsackievirus b and echovirus infections in new-born mice. in addition to previously reported findings, we have sometimes observed degenerative and inflammatory changes in neurons related to the gasserian, posterior root or autonomic ganglia. not all deposits of brown fat are simultaneously the seat ...	1987	2822811
a novel presentation of coxsackie b2 virus infection during pregnancy.	a 39-year-old woman presented at 34 weeks gestation with a febrile illness. this initially settled but recurred 6 days later, when uncontrolled vaginal bleeding necessitated caesarian section. at operation, vesicles were seen on the mother's hyperaemic uterus. the infant developed tachypnoea, bradycardia, cerebral irritation, an enlarged liver and spleen, intravascular coagulation and a transient rash. mother and child made a full recovery. coxsackie b2 virus was isolated from the infant and ris ...	1987	2822813
coxsackie b2 virus causing simultaneous hand, foot and mouth disease and encephalitis.		1987	2822814
possible mechanisms in the pathogenesis of virus-induced diabetes mellitus.	insulin-dependent diabetes mellitus results from destruction of pancreatic beta cells. viruses and autoimmunity have been implicated as possible causes of beta cell destruction in genetically predisposed individuals. the evidence for viruses comes largely from experiments in animals, but several studies in humans point to viruses as triggers in the pathogenesis of diabetes in some cases. in animal models, at least 4 different possible mechanisms for virus-induced diabetes have been proposed. the ...	1987	2824113
cardiac myosin and autoimmune myocarditis.	infection with type 3 of the group b coxsackieviruses (cb3) sometimes leads to the development of myocarditis in humans. circumstantial evidence in the form of heart-reactive antibodies in these cases of human myocarditis suggests that the later phases of the disease may be due to autoimmunization. since human myocarditis is a relatively rare sequel to infection with cb3 virus, we propose that it reflects a genetic predisposition in some individuals. to investigate this possibility we establishe ...	1987	2824143
coxsackie virus infection in association with heart affection in man.	the frequency of actual infections by selected types of coxsackie virus (b1-b6, a7, a9) has been followed up in adult patients hospitalized for reason of heart disease. criteria of actual coxsackie virus infection have been defined, including a significant rise of titer of virus neutralizing antibodies and/or presence of virus-specific igm antibodies. an actual coxsackie virus infection has been found in 71 (46.3%) out of 153 patients. most frequently coxsackie b1 virus infection (24 times) and ...	1987	2824602
virus infection and knee injury.	serological evidence of virus infection was sought in 31 consecutive patients presenting with knee swelling and compared with age/sex-matched controls. in a normal age/sex-matched control group, 42% of patients had evidence of recent or past infection with coxsackie b virus, emphasising the care required in the evaluation of the significance of coxsackie b neutralization titres in individual patients. of 12 patients presenting with knee swelling and a history of a twisting injury, eight had sero ...	1987	2825728
viral infections in beta-thalassemia/hemoglobin e patients.	one hundred ten patients with beta-thal/hb e disease and 60 normal controls matched for age and socioeconomic status were followed for 1.5 years. they were examined clinically, and blood and plasma were studied for coxsackie b viruses and others. the findings suggest that the patients are more susceptible to coxsackie b virus but not to rubella, herpes simplex, cytomegalovirus, adenovirus, and m. pneumoniae. in contrast to bacterial infections, splenectomized patients did not show evidence of in ...	1987	2825835
persistent infection of yac-1 cells by coxsackievirus b3.	persistent infection (pi) of yac-1 cells by coxsackievirus b3 (cbv-3) was characterized. cbv-3 pis were maintained for 7 months or more, although in two other cases cells were cured of virus at 6 and 6.5 months of pi. the titre of infectious virus peaked during the first week of the infection and then gradually decreased. the proportion of cells producing infectious centres increased to 100% by 48 h after infection, remained at that level up to the seventh day, and then rapidly decreased. suscep ...	1988	2826670
effect of astragalus membranaceus injecta on coxsackie b-2 virus infected rat beating heart cell culture.		1987	2827966
the detection of coxsackievirus rna in cardiac tissue by in situ hybridization.	a cloned cdna probe derived from coxsackie b4 virus-infected cell rna was shown to hybridize to the rna of a number of different enteroviruses including coxsackie a and b viruses, echoviruses and poliovirus. the probe was used to detect virus-specific rna sequences in cardiac tissue obtained from patients diagnosed as having a coxsackievirus infection. virus rna was detected using the technique of in situ hybridization in 46% (6/13) cases, but none was found in normal, control, cardiac samples. ...	1988	2828513
human immunodeficiency virus, coxsackievirus, and cardiomyopathy.		1988	2829676
study of mouse spleen "natural cytotoxic" (nc) cell activity related to in vivo administration of b4 coxsackievirus.	by using a2g 40-day-old mice as responders, the influence of in vivo infection with a human strain of b4 coxsackievirus on "natural cytotoxic" (nc) cell activity was assayed, in relation to other immunomodulating treatments (beta-interferon, interleukin-1, or prostaglandin-e2). a significant increase of nc cell cytolysis was noticed in virus-infected mouse group, as compared to the control. the nc cell stimulation exerted by in vivo administration of beta-interferon or of interleukin-1 was not m ...	1987	2830715
[experimental and bacterial conditioned coxsackie b virus-induced pancreatitis].		1987	2830736
natural and lectin-dependent cytotoxicity in virus-induced human myocarditis.	the human spontaneous cell-mediated cytotoxicity (scmc) and lectin dependent cell-mediated cytotoxicity (ldcmc) of peripheral blood leukocytes (pbl) were studied in patients with different forms of postviral myocarditis. the pbl cytotoxicity was evaluated using 51cr-labelled crbc (chicken red blood cells) as targets and results were expressed as a percentage of specific release. the control group was represented by 150 healthy persons of both sexes. no significant age-related differences were fo ...	1987	2830855
asbestos-mediated transfection of mammalian cell cultures.	the capacity of asbestos to mediate transfection was tested in a rapid and relatively simple system: picornavirus rnas and mammalian cells in vitro. thirteen asbestos samples, including amosite, anthophyllite, chrysotile, and crocidolite, 4 picornaviruses (poliovirus 1 and 2, echovirus 7, and encephalomyocarditis virus), and 4 cell lines (cli, chimpanzee liver; kb, human carcinoma; eta, monkey kidney; nih 3t3, mouse embryo) were tested. the results showed that all asbestos samples mediated trans ...	1988	2832359
assessment of recovery efficiency of beef extract reagents for concentrating viruses from municipal wastewater sludge solids by the organic flocculation procedure.	this study was designed to assess the capacity of beef extract reagents to form flocs suitable for virus adsorption. reagent comparisons resulted in the establishment of a modified organic flocculation procedure to concentrate viruses desorbed from sewage sludge solids with currently available modified powdered beef extracts. the method, based on supplementation with paste beef extract floc, achieved virus recoveries comparable to those obtained with powdered beef extract produced before a 1979 ...	1988	2833159
insulin mrna content in pancreatic beta cells of coxsackievirus b4-induced diabetic mice.	molecular hybridization was used to measure poly(a)-containing mrna and insulin mrna, and to evaluate viral persistence, in pancreatic beta cells of coxsackievirus b4-induced diabetic mice. cellular rna was hybridized with [3h]poly(u) to measure poly(a)-containing total mrna, 32p-labeled preproinsulin i and ii probes to measure insulin mrna, and a 32p-labeled virus-specific probe to evaluate persistence. the infected mice (80-90%) showed subnormal blood glucose at 72 h postinfection and were hyp ...	1988	2833417
the relationship between coxsackie-b-virus-specific igg responses and genetic factors (hla-dr, gm, km) in insulin-dependent diabetes mellitus.	igg antibody titres to coxsackie b1-b6 were measured in 113 insulin-dependent diabetes mellitus (iddm) patients whose mean age was 12.2 years and mean duration of iddm was 4.6 years, and in 87 normal sibling controls whose mean age was 13.8 years. compared with their normal siblings, the diabetics had a significantly increased frequency of high response (titre greater than or equal to 320) to coxsackie b2 (8% versus 1%, p = 0.028), to coxsackie b4 (15% versus 1%, p = 0.0006), and to coxsackie b ...	1987	2834133
serological evidence of coxsackie b virus associated with viral cardiovascular diseases in major hospitals of delhi.		1988	2834295
light and electron microscope observation of virus-induced tetrahymena pyriformis in newborn mice (mus musculus albinicus) brain.	newborn mice were infected intracerebrally with abacterial cultures of the gl strain of tetrahymena pyriformis, induced experimentally six years ago in vitro with coxsackie b-5 virus. in the brain of the test animals there were pathologic changes similar to those found in the primary investigation of the pathogenicity of this strain, i.e., after 96 h of contact with the virus. thus, the pathogenicity acquired by t. pyriformis, as well as the persistence of coxsackie b-5 virus in this ciliate, ca ...	1988	2834714
enterovirus in pregnant women and the perfused placenta.	a survey of all nonpolio enterovirus infections in adults for a 10-year period revealed that most such infections will occur in the child-bearing age group during the third quarter of the year. coxsackieviruses occurred more often than echovirus infection. fourteen pregnant women, including four with meningitis, who had an enterovirus infection from 16 to 37 weeks of gestation delivered uninfected, healthy infants. attempts to demonstrate transplacental passage of coxsackie b-3 or echo-11 viruse ...	1988	2834954
coxsackie virus-induced acute pancreatitis in a long-term dialysis patient.	this report describes a patient on continuous ambulatory peritoneal dialysis (capd) who developed acute pancreatitis with pseudocyst formation and cloudy dialysate with pleocytosis in the absence of bacterial or fungal organisms. to our knowledge, pancreatitis, in the absence of hypertriglyceridemia in a capd patient, has not been previously reported. there was a significant increase in coxsackie b1 and b6 viral titers by complement fixation test, suggesting coxsackie virus-induced pancreatitis.	1988	2835903
protective effect of recombinant alpha interferon on coxsackievirus b3 myocarditis in mice.	the effects of recombinant human leukocyte interferon alpha-a/d on experimental myocarditis caused by coxsackievirus b3 were investigated. four-week-old male c3h/he mice were inoculated intraperitoneally with 10(5.5) 50% tissue culture infective dose (tcd50) in 0.1 ml of coxsackievirus b3 (nancy strain). interferon alpha-a/d, 10(4) u/gm/day, was administered subcutaneously daily, starting 1 day before (group 1) or on the day of (group 2) infection. animals were killed on day 7. the infectivity o ...	1988	2837073
t cells are required for coxsackievirus b1 induced murine polymyositis.	murine polymyositis (pm) induced by coxsackievirus b1 (cvb1) has been used as a model of human pm. our study was undertaken to investigate the role of t cells in cvb1 induced pm by examining disease development in neonatally thymectomized mice. clinical weakness and histological inflammatory myositis occurred in 42.7% of sham operated animals but only 7.7% of thymectomized mice. these experiments demonstrate the importance of t cells in the pathogenesis of this virus induced autoimmune disorder.	1988	2837572
importance of virus passage history on the growth of coxsackie b4 in human skin fibroblasts.	human skin fibroblasts have previously been reported to display an age-dependent resistance to infection with coxsackie b4 virus. we have shown that the virus will replicate and produce cpe in human skin fibroblasts regardless of the age of the donor of the cells. the passage history of the virus was found to influence the titre of the virus in these cells.	1988	2838430
use of a-549 cells in a clinical virology laboratory.	a-549 cells were compared with other cell lines for virus recovery, except from specimens submitted specifically for detection of cytomegalovirus. of 589 specimens submitted specifically for detection of herpes simplex virus (hsv), 163 (28%) were positive for hsv--159 (97.5%) in a-549 cells and 156 (96%) in primary rabbit kidney cells. hsv cytopathic effect was identified an average of 0.6 day earlier in a-549 cells. virus was recovered from 194 (11%) of 1,790 specimens submitted for general vir ...	1988	2838502
infection by multiple viruses and lymphocyte abnormalities at the diagnosis of diabetes.	evidence for an ongoing adenovirus infection and preceding eb and coxsackie b virus infections was found in a 2-year-old boy at the diagnosis of insulin-dependent diabetes. an inverted t-helper/t-suppressor lymphocyte ratio and activation of t cells with a suppressor-cytotoxic phenotype were detected. a combination of multiple viral infections may be responsible for the rapid destruction of pancreatic beta cells in this case.	1988	2839003
[immunological characteristics of viral myocarditis].	a study of cellular and humoral immunity was conducted in patients with viral myocarditis. quantitative and functional deficiency of t-suppressors and an increase in the number of cytotoxic lymphocytes were revealed in most patients. disorder in the t-cellular link of immunity correlated with a clinical course of the disease and was found more frequently in patients with a grave course of myocarditis.	1988	2839908
a solid-phase reverse immunosorbent test for the detection of enterovirus igm.	igm antibodies against enterovirus antigen were determined by solid-phase reverse immunosorbent test (sprist). the 145 sera studied were sampled from cases of enterovirus infections diagnosed by virus isolation and/or complement fixation. in 91 sera from enterovirus infections diagnosed by virus isolation 11/40, 16/28 and 9/23 were positive in sprist against echo 3 and/or coxsackie b3 antigen during the first, during the second and third, and after 3 weeks of illness, respectively. the correspon ...	1988	2840453
expression of coxsackievirus b3 capsid proteins in escherichia coli and generation of virus-specific antisera.	subgenomic fragments of cloned infectious coxsackievirus b3 (cvb3) cdna up to the size of the complete coding sequence of the viral polyprotein were inserted into the prokaryotic expression vector pplc24 and expressed in escherichia coli. fusion proteins, containing 54 amino acids of ms2 replicase at their amino terminus followed by different parts of the cvb3 structural proteins, were expressed from several constructs. the expression product of a plasmid encoding the capsid proteins vp4, vp2, a ...	1988	2841081
coxsackie b viruses and myalgic encephalomyelitis.	data collected over the past 6 years suggest that coxsackie b viruses (cbv) play an important role in myalgic encephalomyelitis (me). since psychological upset is a feature of this illness, 247 patients, recently admitted to a psychiatric hospital, were tested for neutralizing antibodies to cbv. a total of 12.5% had significantly raised cbv titres compared with 4-5% of 'well' control groups; the percentage positive was greatest (21%) in those aged 30-39 years. during 1985 and 1986 sera from 290 ...	1988	2841461
[meningomyocarditis caused by coxsackie virus b4 in perinatology. value of anti-coxsackie virus b igm].	the authors report a case of meningocarditis in a neonate caused by a coxsackie virus b4. outcome was favorable. diagnosis was possible by detecting coxsackie b4 specific igm using an elisa test with the sera of the infant and his mother. this assay allows rapid diagnosis and prevention of maternal and neonatal enterovirus infection.	1988	2841914
comparison of five elisa assays for igg antibody against coxsackievirus b1.	enterovirus type and group specificities of five different igg elisa methods were compared, using neutralization titration tests as an indicator of the presence or absence of antibodies to coxsackie b (cb) viruses. one of the elisa assays was a "standard" igg assay, where the solid phase was coated directly with the purified virus, followed by incubations with human serum, biotinylated anti-human-igg, streptavidin-peroxidase, and the substrate/chromogen. in a modified standard assay, blocking of ...	1988	2842447