Publications

clones of cells from a human embryo lung: their growth and susceptibility to respiratory viruses.	colonies of cells were obtained from human fetal lung tissue and exposed to recently isolated respiratory viruses. there was a considerable variation in the number of rounded cells found in different colonies exposed to rhinovirus types 2 and 9 (rv2 and 9), human coronavirus 229e (hcv), adenovirus type 3 (ad3) and respiratory syncytial virus (rsv). smaller colonies had more rounded cells than larger colonies. clones were established from 9 out of 11 colonies. they varied in their rate of growth ...	1979	229795
[method for isolation of enteroviruses from edible mollusca].	a method for recovery of poliovirus from experimentally inoculated mussels has been evaluated. the method, based on adsorption-elution-acid precipitation of virus particles, allows a recovery of more than 93% of the added virus.	1979	229885
the nature of virus-like particles in the paraxial muscles of idiopathic scoliosis.	virus-like particles (vlp) have been identified by electronmicroscopy in the skeletal muscles (paraxials) of six cases of idiopathic scoliosis. these particles closely resembled vlp reported in the skeletal muscles in other conditions, e.g. reye's syndrome, polymyositis, malignant hyperthermia, and chronic myopathy. we have shown by specific staining that these structures are composed of glycogen in a crystalline form. using coxsackie b infected tissue culture cells as a control we have shown th ...	1979	230331
secondary myocardial disease. virus myocarditis and cardiomyopathy.	in an attempt to prove the hypothesis that virus myocarditis may be a cause of idiopathic cardiomyopathy, clinical and experimental studies were performed. eleven patients with a presumptive or proven diagnosis of virus myocarditis were followed for one and a half to 13 years after the acute illness. one patient died in the acute stage, six recovered completely and one continued to have bifascicular block without subjective symptoms. three patients had exertional dyspnea, cardiomegaly and an abn ...	1979	230367
experimental study of virus myocarditis in culture.	in this present paper, the authors would like to present here two points of results from the experimental study concerning virus myocarditis in culture. one is the direct delicate features of heart cells affected with coxsackie b3 (cox. b3) virus viewed through both light microscope and electron microscope. the other is the fact that marked proliferation of fibroblastic cells take place to replace the heart cells impaired by the virus suggesting to us the possibility of one of the genesisses, re ...	1979	230368
an outbreak of diarrhea in school children associated with coxsackie virus b-3 infection.		1979	231430
[persistent non polio enterovirus infections associated with glomerulonephritis. 9 cases (author's transl)].	all patients are excreting the virus in urines and in 4 cases in stools. the virus has also been isolated from the blood. these cases focused on the possible role of enteroviruses as causal agents for the glomerulonephritis.	1979	231760
viral heat resistance and infectious ribonucleic acid.	high-titer suspensions of poliovirus 1 and coxsackievirus b-2 were shown to contain a heat-resistant fraction when heated for 65 min at temperature ranging from 56 to 70 degrees c. the addition of ribonuclease to the heated suspensions eliminated plaque production in the cell cultures, indicating that the resistant fraction was infectious ribonucleic acid that had been liberated from ruptured viruses during the heating process.	1979	231935
adsorption of enteroviruses to soil cores and their subsequent elution by artificial rainwater.	the adsorption and elution of a variety of human enteroviruses in a highly permeable, sandy soil was studied by using cores (43 by 125 mm) collected from an operating recharge basin on long island. viruses studied included field and reference strains of polioviruses types 1 and 3 and reference strains of coxsackie virus b3 and echovirus types 1 and 6. viruses suspended in treated sewage effluent were allowed to percolate through soil cores, and the filtrate was assayed for unadsorbed viruses. to ...	1979	231936
type 1 diabetes and coxsackie virus infection.	the role played by viruses in the aetiopathogenesis of type 1 diabetes mellitus has been studied by several authors; in particular the importance of coxsackie virus b4 infection has been stated by some authors and not confirmed by others. 43 diabetic children were studied at the time of the diagnosis of the disease. no viruses could be isolated from stools; the titres of anti-coxsackie viruses b1 to 6 complement fixing antibodies and anti-coxsackie virus b4 neutralizing antibodies, compared to c ...	1979	232095
enhancement of the antiviral activity of pyrimidine derivatives against mengovirus by visible light.	eleven pyrimido-pyrimidine derivatives, seven with significant antiviral activity against mengovirus, five against coxsackie b1 virus and four antiviral negative compounds were tested for their photosensitizing ability. all seven compounds with antiviral activity in vitro showed an enhanced antiviral action against mengovirus under irradiation with visible light, a fact that may be caused by photodynamic processes. it was tried to correlate the oxidation potentials of sensitizers with their phot ...	1979	232339
development of a simple method for concentrating enteroviruses from oysters.	the development of a simple method for concentrating enteroviruses from oysters is described. in this method viruses in homogenized oyster tissues are efficiently absorbed to oyster solids at ph 5.5 and low salt concentration. after low-speed centrifugation, the supernatant is discarded and viruses are eluted from the sedimented oyster solids by resuspending them in ph 3.5 glycine-buffered saline. the solids are then removed by low-speed centrifugation, and the virus-containing supernatant is fi ...	1975	234154
persistent and fatal central-nervous-system echovirus infections in patients with agammaglobulinemia.	we observed persistent echovirus infection of the central nervous system, as defined by continued presence of isolatable virus in cerebrospinal fluid, in five patients with agammaglobulinemia. the immunologic deficit in each was characterized by absence of surface-immunoglobulin-bearing b lymphocytes and of lymph-node cortical follicles, but normal t-cell function. echoviruses 30, 19, 9 and 33 were recovered from cerebrospinal fluid for periods varying from two months to three years. the patient ...	1977	301244
[echo-11 virus infection in a 6-month-old infant showing clinical signs of varicella].		1979	424258
echovirus 33 replication in human peripheral white blood cells.	echovirus 33 isolated from the cerebrospinal fluid of a patient with agammaglobulinemia was shown to replicate in vitro in peripheral leukocyte suspensions from a normal antibody negative donor. replication was demonstrated by use of sequential dilution experiments. washed glass adherent mononuclear cell cultures from a normal antibody negative donor were also capable of supporting echovirus 33 replication in vitro. leukocyte suspensions from the infected agammaglobulinemic patient extinguished ...	1979	479862
chronic echo type 5 virus meningoencephalitis in x-linked hypogammaglobulinemia: treatment with immune plasma.	a patient with x-linked hypogammaglobulinemia developed chronic meningoencephalitis. echo virus type 5 was repeatedly cultured from cerebrospinal fluid (csf). infusions of high-titer, specific plasma resulted in clinical improvement, but failed to eradicate the virus. after more intensive plasma infusions, the virus could not be cultured from the csf. the patient died 8 months after institution of intensive therapy. the cause of death was unknown. autopsy showed persistence of perivascular and m ...	1979	572004
[encephalomyelitis of atypical course probably caused by echovirus 7].	the authors report a cases of encephalomyelytis caused probably by echo7 virus which was isolated from the cerebrospinal fluid after 45 days of disease. during 120 days 4 relapses of the disease were observed associated with severe general and neurological signs and cerebral coma which ultimately was the cause of death.	1977	593511
[studies of an epidemic episode of serous meningitis caused by echo-virus 30].	the authors report an outbreak of echo 30 aseptic meningitis occurred in marche and umbria countries from november 1975 to june 1976. the clinic, epidemiologic characteristics and the serological and virological results of 28 cases are discussed.	1977	597392
[viral meningoencephalitis: late development].	two years ago, we performed a precocious evolutive eeg study of a specimen not selected from the virus me to echo 4 burst (cepa prima) late 1972 to early 1973 (6). some time between the appearance of the first symptoms and the 8 weeks following, alterations of cerebral electrogenesis became manifest in this study. these alterations disappeared or dimished in significance in the more tardy outlines: focalization tendency, tendency to change from "suffering" to "irritative" aspect. during this yea ...	1977	616553
[production of interferon and virus-neutralizing antibodies in adult mice infected with enteroviruses during the neonatal period].	newborn albino mice were inoculated with enteroviruses and in 6 weeks reinfected with homologous or heterologous strains. strains moderately pathogenic for newborn mice led to formation of specific immunologic memory. mice infected after birth with highly pathogenic strains produced specific antibodies for a long time and lacked secondary response. production of interferon was not associated either with the strain pathogenicity for newborn mice or with the presence of antibodies.	1978	667327
[echo type 30 virus as the etiological agent of meningitis in 1975 in warsaw].		1978	715232
structure and replication of echovirus type 12. 1. analysis of the polypeptides and rna of echovirus 12 particles.	the propagation of echovirus 12 in green monkey kidney (gmk) cells, a continuous cell line, is described. virions and empty capsids are purified to homogeneity by cscl density and by velocity centrifugation. the protein composition of the particles as compared to poliovirus 2 particles is analyzed by dodecyl sulfate/polyacrylamide gel electrophoresis. infectious virions were found to be composed of four different polypeptide chains of molecular weights 33,000, 31,000, 24,000 and 7,000. some addi ...	1978	729593
structure and replication of echovirus type 12. 2. viral polypeptides synthesized in the infected cell.	a technique has been devised which allows us to label predominantly echovirus-12-specific proteins: upon infection in the presence of d-(--)-2-(alpha-hydroxybenzyl)benzimidazole plus actinomycin d the shut-off of host-cell protein synthesis takes place as in infected, untreated cells, but the bulk of viral protein synthesis is inhibited. upon removal of 2-(alpha-hydroxybenzyl)benzimidazole a peak of viral protein synthesis is visible 3 h later. the time course of appearance of viral proteins is ...	1978	729594
myocarditis caused by echo type 11 virus.		1978	757013
electron microscopic study of the morphogenesis of echovirus 23.		1975	803900
febrile neonatal illness associated with echo virus type 5 in the cerebrospinal fluid.		1977	844242
isolation and characterization of an echovirus, intratypic variant of echovirus 33.		1977	851399
the use of rd cells in the isolation of echo type 30 virus from patients with aseptic meningitis.	between early july and august 1976, 60 cases of aseptic meningitis and pharyngitis in children from various parts of west germany were reported to this institute. successful virus isolation from spinal fluid in 12 out of 36 cases was greatly facilitated by the use of rd cell tissue cultures, whereas isolation attempts in hel, vero, and hfdk cells were unsuccessful. in all cases, the isolated virus was identified as echo type 30. neutralization studies with sera of 48 remaining cases also using r ...	1977	875886
echovirus type 7 isolated from conjunctival scrapings.	echovirus type 7 was isolated from 7 conjunctival scrapings obtained during an epidemic of meningoencephalitis caused by the same virus. the patients suffered from conjunctivitis or keratoconjunctivitis, and also had gastrointestinal symptoms, fever, headache and lymphadenopathy early in their illness. two characteristics of the isolated strains were not in agreement with those of the standard echovirus type 7: they adapted only slowly to cell cultures, and no viral hemagglutinin for human red b ...	1977	897572
surface charge of dengue virus hemagglutinins prepared from infected mouse brains.	surface charge of dengue virus hemagglutinin (ha) prepared from infected suckling mouse brains was studied by sucrose gradient electrophoresis. the findings were as follows: (1) both the slow- and rapid-sedimenting hemagglutinins (sha and rha, respectively) prepared after streptomycin treatment of the brain homogenate were distributed broadly within the gradient after electrophoresis, while echovirus type 7 ha which was used as a reference migrated narrowly, suggesting heterogeneity of the surfa ...	1977	916352
severe echo 19 virus infection in a neonatal unit.	an epidemic of echo 19 virus infection in a neonatal unit affecting 12 babies with one death is described. with one exception it was confined to the neonatal unit and medical and nursing staff were also affected. the unit was closed for 9 days, then was disinfected, and there was no recurrence.	1976	962375
enterovirus infections in neonates.	twenty-seven ill neonates with enterovirus infections were studied to learn if enterovirus infection can be distinguished from neonatal sepsis. enterovirus infection was associated with the summer-fall season (93%), recent maternal illness (59%), absence of other perinatal problems (81%), and findings of fever (93%), viral meningitis (62%), diarrhea (81%), and rash (41%). four children developed thrombocytopenia and three necrotizing enterocolitis. three children died, all with coxsackie b virus ...	1976	978329
acute myopathy in three brothers.	three brothers are reported who presented simultaneously with acute myopathy, which resolved rapidly. muscle biopsy showed vacuolar degeneration, with no inflammatory changes. an echo virus was grown from the stools of two of them. their course, together with that of a fourth brother, who had an acute illness with rash, lymphadenopathy and splenomegaly, suggests a possible viral aetiology.	1976	988912
enterovirus infections in acute pancreatitis - a possible etiological connection.	all patients admitted with a preliminary diagnosis of acute pancreatitis during a one-year period were subjected to virus investigations. 142 patients were included in the study and of these 91 were found to have acute pancreatitis. evidence of enteroviral infection was found in 18 of the 91 patients (19.8 per cent). from 17 of the 18 patients enterovirus was isolated from feces or urine, and in one a significant titer rise (cf-test) against coxsackie virus b5 was found. a significant titer rise ...	1976	996429
inhibition of echovirus-12 multiplication by n-carbobenzoxy-d-glucosamine.	the glucosamine derivative, n-carbobenzoxy-d-glucosamine (ncbzg) inhibits the multiplication of echovirus-12 and the synthesis of both virus rna and protein at a stage in the virus growth cycle after attachment and penetration. however, the compound does not inhibit virus multiplication after the appearance of progeny virus nor after virus rna has accumulated. incorporation of radioactive glucosamine and choline into infected and uninfected cultures is inhibited by ncbzg as is the virus-induced ...	1976	1003167
[viral myocardium pericarditis. study of 11 cases].		1976	1031035
epidemic of echovirus 19 in the north-east of england.	we report the first large-scale outbreak of echovirus 19 infection. it occurred in the north-east of england during the summer and autumn of 1974. the virus was isolated from 268 patients in the region. the infection spread from the urban to more rural areas, reaching a peak in mid-august. males were affected more often than females in the ratio 1-6:1. half of the patients were under eight years of age, relatively few were over 35 years. aseptic meningitis and upper respiratory infections were t ...	1976	1063219
myocarditis associated with echo type 7 infection in a leukemic child.	a 3 1/2-year-old boy, during the course of acute lymphocytic leukemia presented clinical, roentgenological and ecg signs of myocarditis, which disappeared completely within 1 1/2 month. echo type 7 virus was isolated from the faeces during the acute stage of the disease and rise in echo 7 neutralizing antibodies was demonstrated in paired sera of the patient. this unusual pathogenicity of echo 7 virus could be explained with the impairment of the host resistance induced by leukemia and immunosup ...	1976	1066956
an epidemic of echovirus 18 meningitis.	the first reported outbreak of echovirus 18 meningitis in the united states occurred during the summer of 1972 in durham, north carolina. one hundred three cases of aseptic meningitis were seen at duke university medical center over a period of four months. most of the patients were less than 25 years old, black, and residents of durham county or nearby counties.. symptoms included headache (92%), fever (76%), nuchal rigidity (67%), and nausea and/or vomiting (51%). in contrast to previously pub ...	1975	1167369
electrophoretic analysis of capsid and non-capsid polypeptides of echovirus 12, and selective inhibtion of the formation of virus particles by actinomycin d.	electrophoretic analysis of purified echovirus virus particles yielded four polypeptides of mol. wt. 37000, 30000, 25000 and 7600. the 75s empty capsids of echovirus 12 lack the 25000 and 7600 mol. wt. polypeptides, and possess polypeptides of 41000 mol. wt. a total of 14 virus-induced polypeptide species was found in the cytoplasm of infected cells. actinomycin d reduced the synthesis of virus, virus rna, and virus polypeptides and also reduced the proportion of virus particles to empty capsid ...	1975	1168242
virologic and serologic studies on an outbreak of echovirus type 11 infection in a hospital maternity unit.	an outbreak of echovirus type 11 (e-11) infection occurred among newborn babies in a hospital maternity unit in the summer of 1971. the results of studies are as follows: 1) forty-one of 188 infants developed febrile illness with stomatitis during one and a half months from july to september. e-11 was isolated from stool specimens of 14 infants and two throat swabs. antibody response to the virus was shown in all the 19 cases examined. some of their mothers were suffering from subclinical infect ...	1975	1195517
a comparative study of meningoencephalitis epidemics caused by echovirus type 7 and coxsackievirus type b5. clinical and virological observations during two epidemics in northern sweden.	two epidemics of meningoencephalitis caused by echovirus type 7 and coxsackievirus type b 5 in the summer and autumn of 1973 in umeå in northern sweden were compared. most patients with echovirus 7 meningoencephalitis were neck stiff and 50% had a polymorphonuclear pleocytosis in the cerebrospinal fluid (csf). the illness was usually mild. it appears to be the first time that an epidemic caused by this virus is described from scandinavia. on the other hand most patients with coxsackievirus b 5 m ...	1975	1209167
studies on some biological properties of heteroploidal cell -- pzh-1 line derived from human embryo lung.	the morphological and karyological properties as well as susceptibility to virus strain of tissue culture derived from human embryo lung that underwent a transformation becoming a continuous line defined as pzh-1 were observed. the observations cover 120 passages divided into four phases: one before and three after transformation. each of these phases was characterised by different biological strain properties. loss of pzh-1 susceptibility to echo virus was the first change of biological propert ...	1975	1211221
[the value of the coxsackie b virus complement fixation test (author's transl)].	enteroviruses were isolated from 27 patients during 1973 and 1974, 21 of these were coxsackie b, 4 were echo and 2 were polio viruses, 5 further viruses could not be identified. sera from 14 of the coxsackie patients were investigated twice by means of the complement fixation test (cft). 11 of them showed a significant rise in antibody titre against one of the 6 different coxsackie b viruses. no strong correlation was established between the result of the cft and the isolated type of virus. neve ...	1975	1226771
protective effect of a monoclonal antibody specific for an echovirus cellular receptor in human fibroblast and simian kidney cell lines.	we previously reported that infection of kb cells by echoviruses (ev) was inhibited by a kb-derived ev receptor murine monoclonal antibody (mab 143). this antibody enabled the identification of a cellular receptor common to all echoviruses (with the exception of ev-22 and -23) and coxsackievirus (cv) a9, but different from the receptor of other picornaviruses. we now present results of cell protection assays conducted with human and simian cell lines different from the kb cell line used for prod ...	1992	1297174
ongoing enterovirus-induced myocarditis is associated with persistent heart muscle infection: quantitative analysis of virus replication, tissue damage, and inflammation.	coxsackievirus b3-induced myocarditis in different immunocompetent mouse strains was used as a model to investigate interrelationships between virus replication and development of chronic enteroviral heart disease. using in situ hybridization to detect enteroviral rna, we show that heart muscle infection is not only detected in acute myocarditis but is also detected during the chronic phase of the disease. coxsackievirus b3 could evade immunological surveillance in a host-dependent fashion, thus ...	1992	1309611
viral myocarditis--a tale of two diseases.		1992	1309925
enteroviral infection of mice with severe combined immunodeficiency. evidence for direct viral pathogenesis of myocardial injury.	inbred mice with genetically determined severe combined immunodeficiency (scid) lack mature t and b lymphocyte functions. to distinguish direct viral effects in the pathogenesis of myocarditis from those mediated by antigen-specific and histocompatibility-complex-restricted host immunity, we inoculated coxsackievirus b3 into homozygous young adult scid mice. we found that infected scid mice invariably developed extensive myocarditis between 7 and 14 days postinoculation with high subsequent mort ...	1992	1309927
association of heat shock protein 70 with enterovirus capsid precursor p1 in infected human cells.	members of the human heat shock (hsp) family of related proteins are involved in the intracellular folding, transport, and assembly of proteins and protein complexes. we have observed that human heat shock protein 70 (hsp70) is associated with the capsid precursor p1 of poliovirus and coxsackievirus b1 in infected hela cells. antiserum generated against hsp70 coimmunoprecipitated the poliovirus protein p1, an intermediate in capsid assembly. similarly, alpha-virion serum coimmunoprecipitated hsp ...	1992	1310763
cytotoxic and viral neutralizing antibodies crossreact with streptococcal m protein, enteroviruses, and human cardiac myosin.	the development of autoimmunity in certain instances is related to infectious agents. in this report, cytotoxic monoclonal antibodies (mabs) that recognize epitopes on both enteroviruses and the bacterium streptococcus pyogenes are described. murine anti-streptococcal mabs that were crossreactive with streptococcal m protein, human cardiac myosin, and other alpha-helical coiled-coil molecules were found to neutralize coxsackieviruses b3 and b4 or poliovirus type 1. the viral-neutralizing anti-st ...	1992	1311095
search for coxsackievirus b3 rna in idiopathic dilated cardiomyopathy using gene amplification by polymerase chain reaction.	a polymerase chain reaction (pcr) amplification assay was developed to detect coxsackievirus b3 ribonucleic acid (rna) in blood and myocardial tissue of explanted hearts from 40 patients who underwent cardiac transplantation and in 1 normal heart. twenty-one patients were affected by idiopathic dilated cardiomyopathy of different duration and 19 by coronary artery disease. coxsackievirus b3 in vitro infected vero cells and cells infected by related human enteroviruses (coxsackievirus b2, b4, and ...	1992	1311139
crystallization and preliminary x-ray diffraction studies of coxsackievirus b1.	preparations of coxsackievirus b1 (cvb1) derived from an infectious cdna clone have been crystallized in multiple crystal forms. using high intensity synchrotron radiation, an orthorhombic form of the crystals was shown to diffract x-rays to at least 2.9 a resolution. the unit cell has a primitive lattice with dimensions a = 323 a, b = 450 a, and c = 522 a. a crystallographic asymmetric unit of these cvb1 crystals probably contains an entire virus particle, implying the presence of 60-fold non-c ...	1992	1311388
focal ventricular thinning caused by indomethacin in the late phase of coxsackievirus b4 murine myocarditis.	indomethacin has been shown to increase virus titers and to worsen cardiac injury in the acute phase of coxsackievirus b4 murine myocarditis. the authors evaluated the effects of indomethacin on the histopathologic changes in a later phase of this disease after virus clearance. two-day old cd1 mice were infected with coxsackievirus b4. ten days later, surviving animals were randomized to receive indomethacin or saline intraperitoneally for 10 days. they were then euthanatized, and their hearts w ...	1992	1311498
mumps virus infects beta cells in human fetal islet cell cultures upregulating the expression of hla class i molecules.	the ability of mumps virus to infect pancreatic beta cells and cause alterations in their hla expression was evaluated in cultured human fetal islet cell clusters. mumps virus could be isolated during the whole culture period (6-8 days) and 60% of cells, including beta cells, contained viral nucleocapsid protein at the end of the culturing. a minor decrease in insulin secretion was observed in some of the infected cultures. the infection was invariably associated with an increase in the expressi ...	1992	1311693
detection of coxsackie b virus myocarditis on tc-99m pyp myocardial imaging.		1992	1312000
coxsackievirus b3-induced production of tumor necrosis factor-alpha, il-1 beta, and il-6 in human monocytes.	infections by coxsackievirus b3 (cvb3) have previously been shown to cause acute and chronic myocarditis characterized by a heavy mononuclear leukocyte infiltration and myocyte necrosis. because clinical and experimental evidence suggested that cardiac damage may result from immunologic rather than viral mechanisms, we examined in this study the in vitro interaction of cvb3 with human monocytes. cvb3 was capable of infecting freshly harvested monocytes as revealed by immunofluorescence and relea ...	1992	1312105
the possible role of coxsackie a and echo viruses in the pathogenesis of type i diabetes mellitus studied by igm analysis.	igm antibodies to coxsackie b virus (cbv) have recently been observed in the serum of a relatively high proportion of children with newly diagnosed insulin-dependent diabetes mellitus (iddm). in the present study, 108 iddm patients below the age of 15 years, diagnosed during the period 1976 to 1985, were investigated at the onset of their disease by mu-antibody-capture radioimmunoassay (ria) of igm against seven different enterovirus antigens, namely virions of cbv serotypes 1-5 (cbv 1-5) and pr ...	1992	1312561
viral genomic detection in the hearts of c3h/he mice with experimental coxsackievirus b3 myocarditis by gene amplification using the polymerase chain reaction.	on the basis of the detection of the enteroviral rna in the hearts of patients with healed myocarditis and dilated cardiomyopathy, we investigated cardiac viral persistence in experimental myocarditis. weanling c3h/he mice were given myocarditis by inoculation with coxsackievirus b3 (nancy strain), and their hearts were examined by genomic studies and viral isolation up to the 180th day after inoculation. the virus was isolated from the heart until the 9th day. by slot-blot hybridization, viral ...	1992	1312649
a mouse model of coxsackievirus myocarditis.	to develop a mouse model of coxsackievirus b3 (cvb3) myocarditis.	1992	1313735
correlation between coxsackie b1 virus replication and enhanced invasiveness of shigella flexneri.	coxsackie b1 virus infection enhances the susceptibility of cultured hep-2 cells to shigella flexneri invasiveness. this can be reproduced partially with uv-inactivated virus, particularly the effect observed shortly after viral inoculation. the following phases of viral multiplication were correlated with the enhancing effect: uncoating of viral particles, synthesis of viral rna and proteins, and assembly of newly produced virus particles. uncoating of virus particles was completed within 60 mi ...	1992	1314061
coxsackie b virus igm in children at onset of type 1 (insulin-dependent) diabetes mellitus: evidence for igm induction by a recent or current infection.	thirty-five children with newly-diagnosed type 1 (insulin-dependent) diabetes mellitus and their 47 siblings were investigated for the presence of igm antibodies to coxsackie b virus serotypes 1-5 (cbv 1-5) with the aid of mu-antibody-capture radioimmunoassays. when a high cut-off value was used, 16 (46%) diabetic children and 16 (34%) siblings showed cbv-igm. of the siblings of diabetic patients positive for cbv-igm, 11 (44%) were cbv-igm-positive; the corresponding figure for the siblings of n ...	1992	1314203
persistent rubella-specific igm reactivity in the absence of recent primary rubella and rubella reinfection.	between two and seven sera from cases of persistent detection of rubella-specific igm for periods in excess of 2.5 months, but in the absence of recent primary rubella or rubella reinfection, were examined for rheumatoid factor, heterophile antibody, and igm reactivity against toxoplasma and a number of viruses. the relative avidity of the rubella-specific igg1 has been assessed in all the sera by two methods. none of the sera contained rheumatoid factor or heterophile antibody, nor did any cont ...	1992	1314284
lipopolysaccharide suppresses cytokine release from coxsackie virus-infected human monocytes.	infections by coxsackie virus b3 (cvb3) have been reported to be associated with an enhanced influx of mononuclear leukocytes into afflicted tissue. current evidence indicates that monocytes/macrophages are specifically involved in cvb3-induced myocarditis by maintaining a chronic inflammatory response. to examine susceptibility and reactivity to cvb3, freshly isolated human monocytes were exposed to various virus doses (0.1-10 moi) in the presence or absence of macrophage-activating lipopolysac ...	1992	1314406
increased frequency of coxsackie b virus igm in women with spontaneous abortion.	igm antibodies to coxsackie b virus serotypes 1-5 (cbv 1-5) were studied by radioimmunoassay (ria) of blood samples from women who had undergone a spontaneous abortion or given birth to a stillborn child. results were compared with those of controls comprising healthy pregnant women who had not suffered such experiences. among 48 women with abortions, 16 (33%) had cbv-igm, while among the controls only three of 37 (8%) had these antibodies, a statistically significant difference (p less than 0.0 ...	1992	1314861
mobilization of cutaneous immunity for systemic protection against infections.	this laboratory reported that a single subcutaneous (sc) injection of the natural steroid hormone dehydroepiandrosterone (dhea) resulted in significant protection against a lethal herpes virus type 2 encephalitis or a systemic coxsackievirus b4 infection. our previous results have shown that sc injection of dhea resulted in upregulation of the specific host immune response resulting in protection against a lethal infection. this hormone did not have any direct antiviral effects in vitro. further ...	1992	1318664
coxsackie b1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness.	to analyze the possible mechanisms by which coxsackie b1 virus infection affects the invasiveness of shigella flexneri, we have studied the influence of intracellular levels of na+ and k+, atpase activity, cytoplasmic membrane potential, camp level and cell communication through gap junctions. 3h after adsorption of viable or uv-inactivated coxsackie b1 virus the na(+)-k+ gradient of the cell collapsed, atpase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion ...	1992	1318709
identification of candidate sequences that determine virulence in coxsackievirus b4.	we have previously shown that a major determinant of virulence for coxsackievirus b4 mapped to the 5' end of the viral genome. comparison of the corresponding cdna sequences of a virulent and a non-virulent virus has allowed the identification of candidate determinants of virulence in the 5' untranslated region and the capsid proteins vp1, vp2 and vp4. thirteen nucleotide substitutions were observed in a region spanning 3298 nucleotides. four mutations were detected in the non-coding region. of ...	1992	1320798
evaluation of the effects of low molecular weight heparin on inflammation and collagen deposition in chronic coxsackievirus b3-induced myocarditis in a/j mice.	coxsackievirus, group b, type 3 (cvb3) infection of a/j male mice induces chronic myocarditis with increased interstitial fibrosis and collagen deposition. heparin, a naturally occurring sulfated glycosaminoglycan, has both anti-inflammatory and antifibrotic activities besides its well-known anticoagulant activity. this study determined whether heparin treatment could decrease either cardiac inflammation or fibrosis in chronic cvb3-induced myocarditis. control mice were either untreated or treat ...	1992	1321562
immunoglobulin class and subclass-specific monoclonal antibody sandwich elisa for the detection of antibodies against coxsackieviruses b, types 1-5.	immunoglobulin subclass-specific elisas were developed for human igg1, igg2, igg3, igg4, igatotal, and igm directed against coxsackie b (cb) virus types 1, 2, 3, 4, and 5. in all the assays the solid phase was coated with immunoglobulin class/subclass-specific monoclonal antibodies, followed by an incubation with the serum specimens. incubation with one of the cb viruses, as well as an incubation with biotinylated serotype-specific monoclonal antibodies to the same virus type provided the virus ...	1992	1321835
acute sensory and autonomic neuropathy: possible association with coxsackie b virus infection.	this report describes a 26 year old woman with a coxsackie b virus infection complicated by an acute pandysautonomic and sensory neuropathy. electrophysiological studies suggested an axonal neuropathy. a sural nerve biopsy performed early in the disease showed axonal degeneration with a virtual absence of unmyelinated fibres and moderate loss of myelinated fibres, mainly affecting the small fibres; this differs from previous reports. an immune-mediated or direct virus action might explain the pa ...	1992	1322452
mumps and coxsackie b3 virus infection of human fetal pancreatic islet-like cell clusters.	islet-like cell clusters (iccs) prepared from human fetal pancreases were infected with mumps or coxsackie b3 virus. double-labeled antibody technique showed that the viruses infected both insulin-secreting and other pancreatic cells and that secretion of immunoreactive insulin into the culture medium of the mumps virus-infected cells had already ceased on day 7. the mumps virus-infected icc clusters produced virus for 14 days, and the mumps virus antigen was detected in the iccs through the who ...	1992	1322538
heat-shock protein induction in adriamycin and picornavirus-infected cardiocytes.	both chemicals, such as the chemotherapy agent adriamycin, and viruses, such as the picornaviruses coxsackievirus b3 and encephalomyocarditis virus, cause metabolic injury in myocardial cells. this injury includes depression of cellular rna and protein synthesis and production of oxygen free radicals which are known to induce increased expression of "heat-shock" or stress proteins (hsp). these hsp can stimulate potent t lymphocyte responses that may then contribute to cardiac damage associated w ...	1992	1323730
successive infection of coxsackievirus b3 and encephalomyocarditis virus: an animal model of chronic myocarditis.	successive infection of coxsackievirus b3 and encephalomyocarditis virus was investigated as a disease model of chronic myocarditis. four-week-old c3h/he mice were inoculated with coxsackievirus b3 and then inoculated with encephalomyocarditis virus at 8 weeks old. the hearts were evaluated on histopathological changes compared with those of non-infected mice and mice infected with either virus alone. at 10 weeks old, the hearts of the mice infected successively with both viruses showed co-exist ...	1992	1325552
[ocular histoenzymatic research on an experimental viral attack].	we sought to investigate enzyme response appearing subsequent to sub-conjunctival administration of the coxsackie b3 virus. this virus stimulates oxidising enzymes, diaphorase and leucin aminopeptidase, dihydrofolate reductase, and adenosinetriphosphatase. the most typical enzyme changes are been in the chorion of the conjunctival mucose and the corneal parenchyma there by showing that the virus, triggers local immune defence mechanisms. the appearance of highly active langerhans cells around bo ...	1992	1325834
[diaphorase activity at the conjunctival level in an experimentally produced viral infection].	by coxsackie virus type b3 subconjunctival administration it has been noticed the appearance of a antiviral defense mechanism, revealed by the presence in conjunctival chorion of lymphocytes, plasmocytes and active macrophages. it had also been studied by histochemical methods the responsive way at conjunctival level of nadh2-cytocrom-c-reductase (diaphorase) in experimentally obtained viral aggression. the injected virus has determined the stimulation of the activity of this enzyme at epithelia ...	1992	1325835
non-cytopathic infection of rhabdomyosarcoma cells by coxsackie b5 virus.	infection of rhabdomyosarcoma (rd) cells by coxsackie b5 virus (cbv5) was non-cytopathic, although low titres of infectious virus were produced after 24 h post-infection. the extent of cbv5 replication in rd cells increased after sequential passage of the virus in these cells. the rd cells from the first cycle of cbv5 infection were recovered and maintained in culture for 3 months (equivalent to 21 passages) releasing infectious virus throughout this period; these cells were considered to be per ...	1992	1326261
mapping of the rd phenotype of the nancy strain of coxsackievirus b3.	the rd variants of group b coxsackieviruses differ from their parental strains in having the ability to replicate in a human rhabdomyosarcoma cell line, rd. the nucleotide sequence of the p1 region of the rd variant of coxsackievirus b3 strain nancy (cb3nrd) was determined by sequencing cloned cdnas, obtained by pcr amplification. a comparison between the established nucleotide sequence and that of the p1 region from the parental virus revealed 12 point mutations which corresponded to six amino ...	1992	1326828
[possible relationship between acute viral myocarditis and dilated cardiomyopathy].	to explore the possible relationship between acute viral myocarditis and dilated cardiomyopathy (dcm), 35 acute diffuse viral myocarditis (advm) patients with cardiac enlargement were studied for 6 years on average. the results showed that: (1) in 22 advm patients, the dilated hearts had returned to normal on x-ray films. the other 13 cases still had cardiac enlargement complicated with various degrees of cardiac insufficiency (nyha ii/iii) and ecg abnormalities. the manifestation of these 13 pa ...	1992	1327706
spectrum of monoclonal antibodies to coxsackievirus b-3 includes type- and group-specific antibodies.	fifteen monoclonal antibodies (mabs) made to coxsackievirus b-3 were tested against a panel of enteroviruses by indirect immunofluorescence. the mabs included seven which reacted with coxsackievirus b-3 only, five which reacted with more than one enterovirus included in the panel, one which reacted with broad enteroviral specificity and did not react with any heterologous virus (group specific), and two which reacted with all enteroviruses tested and at least one heterologous virus. the group-sp ...	1992	1328290
synergistic interaction of interferon-beta and interferon-gamma in coxsackievirus b3-infected carrier cultures of human myocardial fibroblasts.	the antiviral effects of human interferon-beta (ifn-beta) and human recombinant interferon-gamma (rifn-gamma) were studied in persistently coxsackievirus b3-infected carrier cultures of human myocardial fibroblasts over a period of 21 days. synergism was observed with concentrations as low as 30 iu of ifn-beta plus 10 iu of rifn-gamma/ml, reducing mean viral titers from 6.0 x 10(7) to 1.3 x 10(4) pfu/ml and number of infected cells from 14.4% to 0.1% as determined by quantitative in situ hybridi ...	1992	1328409
combination treatment with ribavirin and interferon for coxsackievirus b3 replication.	the effects of combined treatment with ribavirin and recombinant human leukocyte interferon-alpha a/d against coxsackievirus b3 replication were investigated in cultured cells. recombinant human leukocyte interferon-alpha a/d was applied 12 hours before coxsackievirus b3 inoculation and ribavirin was applied 1 hour after coxsackievirus b3 inoculation on fl (human amnion) cell monolayers. these drugs inhibited coxsackievirus b3 replication synergistically by plaque-reduction assay. this method of ...	1992	1328433
t cells expressing the gamma delta t-cell receptor potentiate coxsackievirus b3-induced myocarditis.	initial studies determined whether intraperitoneal (i.p.) injection of balb/c mice with 0.1, 1.0, and 10 mg of adriamycin (a cardiotoxic anthracycline antibiotic) for times ranging between 1 and 9 weeks prior to i.p. injection of 10(5) pfu of coxsackievirus b3 (cvb3) would alter the severity of virus-induced myocarditis. prior adriamycin exposure enhanced pathogenicity of a poorly pathogenic cvb3 variant (h310a1) but had no effect on myocarditis produced by the pathogenic variant (h3). cardiac v ...	1992	1328680
interferon treatment reduced adherence, invasiveness and intracellular multiplication of shigella flexneri in coxsackie b1 virus-infected cells.	the effect of interferon treatment on interaction of shigella flexneri with in vitro cultured cells was investigated. pretreatment of hep-2 cells with human interferons had no effect on the susceptibility of cells to s. flexneri, measured by invasiveness and adhesiveness. human leukocyte interferon and human recombinant interferon-alpha-a reduced adhesiveness, intracellular multiplication and invasiveness of s. flexneri in hep-2 cells preinfected with coxsackie b1 virus. also non-receptor mediat ...	1992	1329437
extracellular matrix remodelling after coxsackievirus b3-induced murine myocarditis.	weanling inbred balb/c mice were intraperitoneally inoculated with a myocarditic variant of coxsackievirus b3. at days 1, 2, 4, 6, 8, 10, 14, 24 and 30 post-infection (p.i.), myocardial tissue was harvested for viral infectivity titrations and histological studies, including routine techniques (haematoxylin-eosin, masson trichrome and von kossa) and specialized procedures (silver impregnation for reticulin, picrosirius red stain for collagen and immunoperoxidase labelling for laminin). virus was ...	1992	1329915
[acute malignant myocarditis and pleurodynia as the manifestation of a probable coxsackie b viral infection].	the authors describe a 45-year-old female patient suffering from rapid-progressing myocarditis with growing disorders in conduction, repolarization alterations and heart dilatation seen for 3 days. the patient died on the 10th day of the disease which started from fever and catarrhal phenomena. attack-like pains in the right half of the chest and abdomen, removable by narcotics, were a remarkable disease manifestation. the diagnosis of myocarditis was verified morphologically. the disease may be ...	1992	1332204
demonstration of the distribution of coxsackie virus rna in neonatal mice by non-isotopic in situ hybridization.	a simple method for the demonstration of coxsackie virus rna by in situ hybridization is described. oligonucleotides complimentary to conserved sequences of coxsackie b genome were synthesised and labelled with digoxigenin using commercially available reagents. in addition to detecting all five coxsackie b strains examined, six strains of coxsackie a were also demonstrated by these probes. using one of the oligonucleotides separately it was possible to distinguish coxsackie a strains from the st ...	1992	1333472
[the effect of a coxsackie viral infection on the chromosomal apparatus of the cells of fetuses and newborn mice].	the coxsackie virus b1 has been studied for its effect on the chromosome apparatus in cells of mouse feti and newborn mice in case of intraperitoneal infection of females on the 3d week of pregnancy. it is stated that the virus readily moves across the placental barrier and induces chromosome aberrations in the cells of feti, their number being 2.5 times higher than the control level. the maximal number of structural chromosome aberrations was registered during the first three days from the mome ...	1992	1333568
virus infection and hand papular dermatoses in young children.	four strains of enterovirus and 1 strain of adenovirus were isolated from stool and pharyngeal swabs by inoculation into primary human embryonic kidney cell culture in 25 cases of dorsum manus infantile papular dermatitis. two strains of coxsackie a-9 virus and 1 strain of echovirus-25 were identified. with the isolated virus as antigen, a matched pair study by sera antibody test was carried out in 43 children with papular dermatitis and 40 normal individuals as controls. in 26 cases of coxsacki ...	1992	1333941
coxsackievirus b4 infection alters thymic, splenic, and peripheral lymphocyte repertoire preceding onset of hyperglycemia in mice.	diabetogenic coxsackievirus b4 infection may trigger autoimmune islet loss in diabetes-susceptible mice, resulting in hyperglycemia in nearly 90% of the animals at 6-8 weeks postinfection (p.i.). to ascertain whether changes in lymphocyte repertoire following infection could predispose these animals to diabetes, alterations in their thymic, splenic, and peripheral lymphocytes were analyzed. additionally, lymphocyte changes were correlated with the virus load in these tissues and with lymphocyte ...	1992	1334127
placental phagocytic cells infected with herpes simplex type 2 and echovirus type 19: virological and ultrastructural aspects.	placental macrophage cells were kept in a short-term culture and infected with herpes simplex type 2 virus and echovirus type 19. these were observed under optical and electron microscopy. immunofluorescence, virus titration and autoradiographic technique were used to determine if the virus was replicating in the system. the results showed that the placental phagocytic cells do not allow virus growth and that the virus particles are destroyed right after virus uptake, within 4 h post-infection. ...	1992	1335148
[using dot-immunobinding assay for the determination of coxsackie b virus specific igg in patients with viral myocarditis].		1992	1335409
t-lymphocyte mediated injury of beta cells in dual-aetiology diabetes mellitus in swiss albino mouse.	earlier we had described a dual aetiology diabetes mellitus (dadm) in mice injected with a sub-diabetogenic dose of streptozotocin (sd-szn) and afterwards infected with coxsackie b3 virus (cbv). further experiments were conducted to understand the mechanism of diabetogenesis. in in vitro stimulation and proliferation tests, the splenic lymphocytes (slc) of mice given either sd-szn or cbv infection showed lower responses to two t cell mitogens than those of control mice, indicating an immunosuppr ...	1992	1335964
effects of polyethylene glycol conjugated superoxide dismutase on coxsackievirus b3 myocarditis in mice.	the aim was to examine the role of oxygen derived free radicals in the development of myocarditis by investigating the effects of polyethylene glycol conjugated superoxide dismutase (peg-sod), a potent scavenger of oxygen free radicals, upon coxsackievirus b3 (cb3) myocarditis.	1992	1336713
detection of viral rna in experimental coxsackievirus b3 myocarditis of mice using the polymerase chain reaction.	the presence of the viral rna in the myocardium in experimental coxsackievirus b3 myocarditis of mice was investigated using the polymerase chain reaction (pcr). four-week-old c3h/he mice (n = 35) were inoculated with coxsackievirus b3 (nancy strain, 10(5) plaque-forming units/mouse). we used a pair of primers, which encompass a part of the 5' end sequence of the coxsackievirus b3 genome and can also detect many enteroviral rnas. we found that hearts were positive for the viral rna from 2 to 21 ...	1992	1337265
effect of verapamil on acute coxsackievirus b3 murine myocarditis.	the effect of verapamil (ver) on cvb3 murine myocarditis was investigated. it was found that ver could aggravate the myocardial inflammation, increase the viral replication in myocardium, and raise mortality in mice with viral myocarditis when the drug was injected within the first 6 days after the cvb3 inoculation.	1992	1337875
high frequency of igm antibodies to coxsackie b virus in sarcoidosis patients and patients with asbestos-related lesions.	by using radioimmunoassay (ria) for detection of igm antibodies to coxsackie b viruses (cbv), the occurrence of these antibodies was investigated in patients with sarcoidosis and asbestos-related lesions. sixty-one per cent of the patients with sarcoidosis, all patients with benign asbestos pleural effusion, and 67% of those with diffuse asbestos-related pleural thickening showed cbv-igm. patients with healed sarcoidosis or pleural plaques were all negative, and among the "healthy" controls seve ...	1992	1344042
polyclonal immunoglobulin therapy protects against cardiac damage in experimental coxsackievirus-induced myocarditis.	balb/c male mice infected i.p. with 2 x 10(5) plaque forming units (pfu) of coxsackievirus b3 (cvb3) develop severe myocarditis 7 days later. studies were performed to determine whether therapy with normal polyclonal immunoglobulin would prevent cardiac inflammation. partially purified immunoglobulin was derived from pooled mouse serum by ammonium sulphate precipitation. infected animals given either 100 or 1000 micrograms of this preparation for 2 days prior to infection showed greater than 50% ...	1992	1349527
effect of enterovirus infection on susceptibility of hela cells to shigella flexneri invasivity.	invasiveness of shigella flexneri m90t in hela cells was significantly increased when cells were preinfected with poliovirus 1, coxsackievirus b3 and echovirus 6. this effect was dependent on the dose of virus used, evident at early stages of viral infection and lasted hours before the appearance of a cytopathic effect. an increase of bacterial invasion ability was also noticed when hela cells were incubated with uv-inactivated enteroviruses. this enhancing effect obtained with both viable and u ...	1992	1364020
a method for the preparation of purified antigens of coxsackievirus b3 from a large volume of cell culture supernatant.	a simple procedure was used for the concentration and partial purification of coxsackievirus b3 (nancy strain). for a large-scale production of virus. vero cells grown in roller bottles were used. virus concentrate from a large volume of cell culture supernatant was prepared by precipitation with 6% (w/w) polyethylene glycol. this crude antigen was further purified by banding in cesium chloride gradient using ultracentrifugation. the infectivity and haemagglutination activity of virus were check ...	1992	1364026
detection of enteroviruses in cell cultures by using in situ transcription.	in situ transcription (ist) was shown to be useful for the detection of human enteroviral rna in cultured cells. a primer to detect a wide variety of enteroviral genomes and a coxsackievirus type b3 genome-specific primer were demonstrated to be efficient in ist assays. transcription times greater than 10 to 30 min did not significantly improve the acquisition of a specific signal, whereas the signal-to-noise ratio decreased with time. inclusion of actinomycin d to suppress dna-dependent dna pol ...	1992	1370849