Publications

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occurrence of cherry mottle leaf virus in poland. 19695380519
cherry virus a: cdna cloning of dsrna, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry.the nucleotide sequence (7383 nucleotides) of a newly identified member of the genus capillovirus, cherry virus a (cva), was obtained from cdna clones. the cdna was generated from dsrna extracted from plant tissue infected with little cherry virus (lcv). small amounts of lcv dsrna served as template nucleic acid and enabled the construction of a library of which, unexpectedly, 7.5% of the recombinant plasmids were specific for cva. the genome organization of cva resembles that of apple stem groo ...19957636482
single primer pair designs that facilitate simultaneous detection and differentiation of peach mosaic virus and cherry mottle leaf virus.peach mosaic virus (pmv) and cherry mottle leaf virus (cmlv) are viruses which are related serologically and share common prunus hosts, but cause distinct diseases. an rt-pcr procedure using a single oligonucleotide primer pair that allows simultaneous detection and differentiation of the two viruses was developed. a sense primer with 100% complementarity to pmv and 83% complementarity to the corresponding site of the cmlv genome was combined with either of two antisense primers (one of pmv orig ...199910598088
a simple and reliable protocol for the detection of apple stem grooving virus by rt-pcr and in a multiplex pcr assay.primers were identified which amplify specifically a 499 bp fragment in the coat protein coding region of apple stem grooving virus (asgv) genome. these primers were used in various rt-polymerase chain reaction (pcr) analyses for the detection of asgv in chenopodium quinoa, nicotiana occidentalis, and in species of malus and pyrus. isolates of asgv in malus and pyrus from locations in canada, china, israel, japan, nepal, pakistan, south africa, and the u.s.a. were reliably detected in leaf and b ...199910598077
nucleotide sequence and genome organisation of cherry mottle leaf virus and its relationship to members of the trichovirus genus.the nucleotide sequence of cherry mottle leaf virus (cmlv) was determined and compared to sequences of a number of plant viruses including the type member of the trichovirus genus (apple chlorotic leafspot virus, aclsv), and members of the vitivirus genus including grapevine virus b, (gvb). the cmlv genome was determined to consist of 8003 nt excluding the poly(a) tail at the 3' end of the genome. the overall a + u content of cmlv genomic rna was 59.1%, which is similar to aclsv, but significant ...200010881685
molecular characterization, phylogenetic relationships, and specific detection of peach mosaic virus.abstract peach mosaic virus (pcmv) and cherry mottle leaf virus (cmlv) are serologically related viruses that cause distinct diseases, have a different host range, and are vectored by different eriophyid mites. sequence analysis of the genome of pcmv indicates that it is closely related genetically to cmlv but distinct, with similar genome organization and a member of the genus trichovirus. the genome of pcmv consists of 7,988 nucleotides, excluding a poly(a) tail at the 3' end of the genome. fo ...200618943916
molecular variability analyses of apple chlorotic leaf spot virus capsid protein.the complete sequences of the coat protein (cp) gene of 26 isolates of apple chlorotic leaf spot virus (aclsv) from india were determined. the isolates were obtained from various pome (apple, pear and quince) and stone (plum, peach, apricot, almond and wild himalayan cherry) fruit trees. other previously characterized aclsv isolates and trichoviruses were used for comparative analysis. indian aclsv isolates among themselves and with isolates from elsewhere in the world shared 91-100% and 70-98% ...201021289443
complete nucleotide sequence of a strain of cherry mottle leaf virus associated with peach wart disease in peach.the complete nucleotide sequence and genome organization of a peach virus isolate from a naturally infected peach tree showing typical peach wart-like symptoms on the fruit surface was determined and compared to sequences of members of the family betaflexiviridae. the genome consists of 7,987 nucleotides, excluding the poly-a tail, and has four open reading frames (orfs). analysis of the whole genome and putative proteins encoded by each orf revealed greatest sequence similarity to a cherry isol ...201323649175
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