Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
long-term effectiveness of plasma-derived hepatitis b vaccine 22-28 years after immunization in a hepatitis b virus endemic rural area: is an adult booster dose needed?longan county is considered a highly endemic area for hepatitis b virus (hbv). the plasma-derived vaccine has been used in newborns in this area since 1987. a cross-sectional survey was conducted to evaluate the long-term effectiveness of this vaccine. in total, 1634 participants born during 1987-1993 and who had received a series of plasma-derived hb vaccinations at ages 0, 1, and 6 months were enrolled. serological hbv markers were detected and compared with previous survey data. overall the p ...201728065199
ultrahigh pressure-assisted enzymatic extraction maximizes the yield of longan pulp polysaccharides and their acetylcholinesterase inhibitory activity in vitro.an extraction method employing ultrahigh pressure-assisted enzymatic treatment was developed and optimized by response surface methodology to increase the yield of longan pulp polysaccharides (lp-ue). a maximum polysaccharides yield of 8.55% was obtained under the optimal conditions of 407mpa ultrahigh pressure maintained for 6min with an enzyme to pretreated material ratio of 1:100, an enzymolysis time of 1.7h and a water to pretreated material ratio of 42ml/g. subsequently, the physicochemical ...201727908719
differential gene expression of longan under simulated acid rain stress.differential gene expression profile was studied in dimocarpus longan lour. in response to treatments of simulated acid rain with ph 2.5, 3.5, and a control (ph 5.6) using differential display reverse transcription polymerase chain reaction (ddrt-pcr). results showed that mrna differential display conditions were optimized to find an expressed sequence tag (est) related with acid rain stress. the potential encoding products had 80% similarity with a transcription initiation factor iif of gossypi ...201728299408
hydrogen peroxide-induced pericarp browning of harvested longan fruit in association with energy metabolism.energy metabolism of "fuyan" longan fruit treated with hydrogen peroxide (h2o2), the most stable of the reactive oxygen, and its relationship to pericarp browning were investigated in this work. the results displayed that h2o2 significantly decreased contents of adenosine triphosphate (atp) and adenosine diphosphate (adp). it also inhibited activities of h(+)-atpase, ca(2+)-atpase and mg(2+)-atpase in membranes of plasma, vacuole and mitochondria during the early-storage and mid-storage (except ...201728193430
molecular tracing of the origin of six different plant species in bee honey using real-time pcr.the quality of honey is significantly influencedby floral origin. mislabeling floral species occursfrequently in beehoney products. to protectconsumers from economic fraud and maintain afair market environment, methods to identify floralspecies in honey are necessary. in our study, realtimepcrs were established, targeting six honeytypes mainly produced in china (canola, chinesemilkvetch, chinese chaste tree, locust tree, litchi,and longan). sensitivity testing on dna from planttissues exhibited ...201728094000
dnp and atp induced alteration in disease development of phomopsis longanae chi-inoculated longan fruit by acting on energy status and reactive oxygen species production-scavenging system.as compared with p. longanae-inoculated longans, dnp treatment for p. longanae-inoculated longans exhibited higher fruit disease index and pericarp browning index, lower atp amount and energy charge level, lower activities of sod, cat and apx, lower amounts of asa and gsh, lower levels of dpph radical scavenging activity and reducing power, higher o2(-) generating rate and mda amount. however, supply of atp for p. longanae-inoculated longans showed the contrary effects. these results gave convin ...201728317755
volatile organic compounds of thai honeys produced from several floral sources by different honey bee species.the volatile organic compounds (vocs) of four monofloral and one multifloral of thai honeys produced by apis cerana, apis dorsata and apis mellifera were analyzed by headspace solid-phase microextraction (hs-spme) followed by gas chromatography and mass spectrometry (gc-ms). the floral sources were longan, sunflower, coffee, wild flowers (wild) and lychee. honey originating from longan had more vocs than all other floral sources. sunflower honey had the least numbers of vocs. cis-linalool oxide, ...201728192487
neuroprotective activity of cerebrosides from typhonium giganteum by regulating caspase-3 and bax/bcl-2 signaling pathways in pc12 cells.an investigation of the potential neuroprotective natural product constituents of the rhizomes of typhonium giganteum led to the isolation of two new cerebrosides, typhonosides b (1) and c (2), along with 11 known analogues (3-13). the structures of compounds 1 and 2 were elucidated by spectroscopic data interpretation. the activity of these compounds against glutamate-induced cell apoptosis was investigated in pc12 cells. all compounds exhibited such activity, which was related to the length of ...201728394604
genome-wide sequencing of longan (dimocarpus longan lour.) provides insights into molecular basis of its polyphenol-rich characteristics.longan ( dimocarpus longan lour.), an important subtropical fruit in the family sapindaceae , is grown in more than ten countries. longan is an edible drupe fruit and a source of traditional medicine with polyphenol-rich traits. tree size, alternate bearing, and witches' broom disease still pose serious problems. to gain insights into the genomic basis of longan traits, a draft genome sequence was assembled.201728368449
longan seed and peel as environmentally friendly corrosion inhibitor for mild steel in acid solution: experimental and theoretical studies.longan seed and peel were extracted and used for impeding the corrosion of mild steel in hydrochloric acid solution. experimental analyses indicate that the water extract of longan's seed and peel, a mixed type inhibitor, can be chemically adsorbed onto the steel surface, and its inhibition effect rises with increasing extract concentration. to understand the adsorption mechanism and the contributions of the four main compounds of the extract to corrosion protection effect, quantum chemical meth ...201728365436
inhibitory effects of propyl gallate on membrane lipids metabolism and its relation to increasing storability of harvested longan fruit.effects of propyl gallate on membrane lipids metabolism and its relation to storability of harvested longan fruits were studied. the results showed that the propyl gallate-treated longans maintained lower activities of pericarp phospholipase d (pld), lipase and lipoxygenase (lox) than those in control fruits. such treatments could maintain higher levels of pericarp unsaturated fatty acids (usfas), higher pericarp indices of unsaturated fatty acids (iufa), and higher pericarp ratio of unsaturated ...201727664618
[assessment on the yield loss risk of longan caused by cold damage in south china].using daily climate variables gathered from 64 meteorological stations in south china from 1961 to 2012, recognized hazard indicators about disaster grades of cold damage for longan, and methods on agricultural meteorological disasters risk and simulation technology, the yield loss risks of longan caused by cold damage in south china during different developmental periods were assessed. the results showed that during the period of physiologic differentiation of flower bud, the disasters of longa ...201627396131
identification of monofloral honeys using hplc-ecd and chemometrics.a total of 77 jujube, longan and chaste honey samples were collected from 18 different areas of china. thirteen types of phenolic acids in the honey samples were analysed using high-performance liquid chromatography with electrochemical detection (hplc-ecd). moreover, hplc-ecd fingerprints of the monofloral honey samples were established. from the analysis of the hplc-ecd fingerprints, common chromatography peak information was obtained, and principal component analysis and discriminant analysis ...201626471540
identification of differentially expressed genes implicated in peel color (red and green) of dimocarpus confinis.nowadays, there are few reports about regulatory genes implicated in peel color of longan. the basic genetic research of longan has been in stagnation for a long time as a lack of transcriptomic and genetic information. to predict candidate genes associated with peel color, gene functional annotation and coding sequence prediction were used to perform functional annotation for our assembled unigenes and investigate differentially expressed genes (degs) of fruitlet peels from longli (dimocarpus c ...201627468388
impact of precooling and controlled-atmosphere storage on γ-aminobutyric acid (gaba) accumulation in longan (dimocarpus longan lour.) fruit.longan (dimocarpus longan lour.) fruit cultivars 'chuliang' and 'shixia' were analyzed for γ-aminobutyric acid (gaba) accumulation after precooling and in controlled-atmosphere storage. fruit were exposed to 5% o2 plus 3%, 5%, or 10% co2 at 4 °c, and gaba and associated enzymes, aril firmness, and pericarp color were measured. aril softening and pericarp browning were delayed by 5% co2 + 5% o2. gaba concentrations and glutamate decarboxylase (gad; ec 4.1.1.15) activities declined during storage ...201627412947
effect of longan polysaccharides on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro.for autologous chondrocyte implantation (aci) to restore cartilage defect, limited cell numbers and dedifferentiation of chondrocytes are the major difficulties. an alternative is the use of growth factors, but the high cost and potential tumorigenesis are the major obstacles. to ensure successful aci therapy, it is of significance to find effective substituted pro-chondrogenic agent. polysaccharides from plant extract have low toxicity and few undesirable side effects, which were reported to fa ...201626231088
advances on bioactive polysaccharides from medicinal plants.in recent decades, the polysaccharides from the medicinal plants have attracted a lot of attention due to their significant bioactivities, such as anti-tumor activity, antioxidant activity, anticoagulant activity, antidiabetic activity, radioprotection effect, anti-viral activity, hypolipidemic and immunomodulatory activities, which make them suitable for medicinal applications. previous studies have also shown that medicinal plant polysaccharides are non-toxic and show no side effects. based on ...201626463231
validation of reference genes for rt-qpcr studies of gene expression in preharvest and postharvest longan fruits under different experimental conditions.reverse transcription quantitative pcr (rt-qpcr) as the accurate and sensitive method is use for gene expression analysis, but the veracity and reliability result depends on whether select appropriate reference gene or not. to date, several reliable reference gene validations have been reported in fruits trees, but none have been done on preharvest and postharvest longan fruits. in this study, 12 candidate reference genes, namely, cyp, rpl, gapdh, tua, tub, fe-sod, mn-sod, cu/zn-sod, 18srrna, ac ...201627375640
quantification of toxins in soapberry (sapindaceae) arils: hypoglycin a and methylenecyclopropylglycine.methylenecyclopropylglycine (mcpg) and hypoglycin a (hga) are naturally occurring amino acids found in some soapberry fruits. fatalities have been reported worldwide as a result of hga ingestion, and exposure to mcpg has been implicated recently in the asian outbreaks of hypoglycemic encephalopathy. in response to an outbreak linked to soapberry ingestion, the authors developed the first method to simultaneously quantify mcpg and hga in soapberry fruits from 1 to 10 000 ppm of both toxins in dri ...201627367968
antioxidant and catalytic applications of silver nanoparticles using dimocarpus longan seed extract as a reducing and stabilizing agent.in this study, a simple and environmental friendly method was developed for the synthesis of silver nanoparticles (ag-nps) using dimocarpus longan seed extract as a source of reducing and stabilizing agent. the appearance of a surface plasmon resonance peak at 432nm confirmed the synthesis of silver nanoparticles (uv-visible spectroscopy). the biosynthesized ag-nps were face centered cubic structures (xrd) with an approximate particle size of 40nm (tem). optimization study revealed that 10ml of ...201627723492
biosynthesis, antibacterial activity and anticancer effects against prostate cancer (pc-3) cells of silver nanoparticles using dimocarpus longan lour. peel extract.metal nanoparticles, particularly silver nanoparticles (agnps), are developing more important roles as diagnostic and therapeutic agents for cancers with the improvement of eco-friendly synthesis methods. this study demonstrates the biosynthesis, antibacterial activity, and anticancer effects of silver nanoparticles using dimocarpus longan lour. peel aqueous extract. the agnps were characterized by uv-vis absorption spectroscopy, x-ray diffraction (xrd), high-resolution transmission electron mic ...201627316741
evaluation of xanthine oxidase inhibitory potential and in vivo hypouricemic activity of dimocarpus longan lour. extracts.longan is a fruit tree known to contain many phenolic components, which are capable of protecting people from oxidative damage through an anti-inflammatory mechanism. it may be also worthwhile to study the effect on lowering uric acid activity.201627279708
ras-related nuclear protein ran3b gene is involved in hormone responses in the embryogenic callus of dimocarpus longan lour.ras-related guanosine triphosphate (gtp)-binding nuclear protein (ran) gtpases function as molecular switches and regulate diverse cellular events in eukaryotes. our previous work suggested that dlran3b is active during longan (dimocarpus longan lour.) somatic embryogenesis (se) processes. herein, subcellular localization of dlran3b was found to be localized in the nucleus and expression profiling of dlran3b was performed during longan se and after exposure to plant hormones (indoleacetic acid ( ...201627271605
hydrogen peroxide induced changes in energy status and respiration metabolism of harvested longan fruit in relation to pericarp browning.energy status and respiration metabolism of "fuyan" longan fruit treated by hydrogen peroxide (h2o2) and their relationship to pericarp browning were studied. the results displayed that h2o2 significantly increased the respiration rate, increased activities of respiratory terminal oxidases like cytochrome c oxidase (cco) and ascorbic acid oxidase (aao), decreased nad kinase activity, maintained lower contents of nadp and nadph as well as higher amounts of nad and nadh, and accelerated the decrea ...201627213701
molecular cloning, structural and expression profiling of dlran genes during somatic embryogenesis in dimocarpus longan lour.to clone and examine expression profiles of dlran genes during somatic embryogenesis in dimocarpus longan lour. thirty cdna sequences and two genomic sequences encoding dlran proteins were isolated from longan embryogenic cultures. structural analysis of dlran genes revealed that the longan ran gene family is more expanded than that of arabidopsis. expression analysis of dlran genes during somatic embryogenesis uncovered a high abundance of dlran genes in early embryogenic cultures and heart- an ...201627026877
anti-inflammatory effect of longan seed extract in carrageenan stimulated sprague-dawley rats.longan seeds have been used as a folk medicine in china. longan seed extract (lse) is known for antioxidative, antiproliferative, hypoglycemic, and hypouremic effects. however, its anti-inflammatory effect has not been shown.201627746869
bioguided fraction and isolation of the antitumor components from phyllanthus niruri l.phyllanthus niruri l., a well-known medicinal plant, has been used as a folk antitumor remedy in the worldwide scale. however, the antitumor components in p. niruri have not been reported. in order to verify the antitumor components of p. niruri and the plants which have the high content of these components, we isolated the antitumor components with bioguided fraction and isolation, by different chromatographic methods from the ethyl acetate fraction of p. niruri., and identified them as ethyl b ...201627777954
anti-hepatitis c virus activity of a crude extract from longan (dimocarpus longan lour.) leaves.infection with hepatitis c virus (hcv) results in hepatitis c, a disease characterized by chronic infection, cirrhosis, and hepatocellular carcinoma. currently, the standard therapy is a combination of pegylated interferon-α plus ribavirin with ns3 protease inhibitors. addition of ns3 protease inhibitors to the standard therapy improves response rates; however, use of ns3 protease inhibitors is also associated with significant adverse effects and an increase in the overall cost of treatment. the ...201626255732
identification and characterisation of two general odourant-binding proteins from the litchi fruit borer, conopomorpha sinensis bradley.the litchi fruit borer, conopomorpha sinensis bradley, is one of the most destructive pests of litchi and longan fruits in south-east asia and southern china, yet the molecular biology and physiology of this pest remain poorly understood. control of this insect pest may be achieved by interfering with its recognition of host plants.201626085035
photocatalytic and antibacterial response of biosynthesized gold nanoparticles.increase in the bacterial resistance to available antibiotics and water contamination by different toxic organic dyes are both severe problems throughout the world. to overcome these concerns, new methodologies including synthesis of nontoxic, human friendly and efficient nanoparticles is required. these nanoparticles not even inhibit the growth of microorganisms but are also effective in the degradation of toxic organics in waste water thus providing a clean and human friendly environment. the ...201627394010
longan (dimocarpus longan lour.) fruit extract stimulates osteoblast differentiation via erk1/2-dependent runx2 activation.longan (dimocarpus longan lour.) has been used as a traditional oriental medicine and possesses a number of physiological activities. in this study, we used cell-based herbal extract screening to identify longan fruit extract (lfe) as an activator of osteoblast differentiation. lfe up-regulated alkaline phosphatase (alp) activity, induced mineralization, and activated runx2 gene expression in mc3t3-e1 cells. furthermore, treatment of mc3t3-e1 cells with lfe promoted the phosphorylation of extrac ...201626975768
application of longan shell as non-conventional low-cost adsorbent for the removal of cationic dye from aqueous solution.with simple physical treatment, adsorption potential of longan shell for the methylene blue (mb) from aqueous solution was studied as a low-cost material under the conditions of adsorbent dosage (1-6 g/l), initial solution ph (2-12), contact time (5-180 min), temperature (293, 313, 313 k) and initial dye concentration (100-500 mg/l). the sem images and ftir spectra of longan shell before and after dye adsorption were analyzed to understand the adsorption process of mb onto longan shell. the kine ...201626878356
longan (dimocarpus longan lour.) inhibits lipopolysaccharide-stimulated nitric oxide production in macrophages by suppressing nf-κb and ap-1 signaling pathways.flower, seed, and fruit of longan (dimocarpus longan lour.) have been used in the traditional chinese medicine (tcm) serving as a common herb in relief of swelling which can be applied in cases of inflammatory diseases. however, the scientific evidence related to their effects on inflammation especially the possible cellular and molecular mechanisms of longan need to be clarified.201626721218
differential effects of foods traditionally regarded as 'heating' and 'cooling' on prostaglandin e(2) production by a macrophage cell line.some components of natural foods may enhance or inhibit prostaglandin formation and potentially affect the inflammation condition. a macrophage cell line, raw264.7, was employed to examine the effects of foods traditionally regarded as 'heating' or 'cooling' on the production of pge(2), a well-known proinflammatory mediator. foods traditionally regarded as 'heating' (litchi, longan, and dried longan) or 'cooling' (chrysanthemum flower, bitter gourd, and lotus seed plumule) were extracted sequent ...201612432225
developing single nucleotide polymorphism (snp) markers from transcriptome sequences for identification of longan (dimocarpus longan) germplasm.longan (dimocarpus longan lour.) is an important tropical fruit tree crop. accurate varietal identification is essential for germplasm management and breeding. using longan transcriptome sequences from public databases, we developed single nucleotide polymorphism (snp) markers; validated 60 snps in 50 longan germplasm accessions, including cultivated varieties and wild germplasm; and designated 25 snp markers that unambiguously identified all tested longan varieties with high statistical rigor ( ...201526504559
microanalysis, pharmacokinetics and tissue distribution of polysaccharide-protein complexes from longan pulp in mice.a high performance size exclusion-fluorescence detection (hpsec-fd) method combined with fluorescein isothiocyanate (fitc) prelabeling was established for the microanalysis of polysaccharide-protein complexes from longan pulp (lpp). fitc-labeled lpp (lppf) was fractionated by gel filtration chromatography. the weight-average molecular weight and fitc substitution degree of lppf were 39.01 kda and 0.20%, respectively. the hpsec-fd calibration curves linear over the range of 1-200 µg/ml in mouse p ...201526501257
microrna390-directed tas3 cleavage leads to the production of tasirna-arf3/4 during somatic embryogenesis in dimocarpus longan lour.trans-acting short-interfering rnas (tasirnas) originate from tas3 families through microrna (mirna) 390-guided cleavage of primary transcripts and target auxin response factors (arf3/-4), which are involved in the normal development of lateral roots and flowers in plants. however, their roles in embryo development are still unclear. here, the pathway mir390-tas3-arf3/-4 was identified systematically for the first time during somatic embryo development in dimocarpus longan. we identified the mir ...201526734029
two new flavonol glycosides from dimocarpus longan leaves.from the extracts of dimocarpus longan lour leaves, 2 unusual flavonol glycosides, quercetin 3-o-(3″-o-2‴-methyl-2‴-hydroxylethyl)-β-d-xyloside (1) and quercetin 3-o-(3″-o-2‴-methyl-2‴-hydroxylethyl)-α-l-rhamnopyranoside (2), as well as 10 known compounds including 2 flavonol glycosides, afzelin (3) and kaempferol-3-o-α-l-rhamnopyranoside (4), 2 flavans, (-)-epicatechin (5) and proanthocyanidin a-2 (6), 3 triterpenoids, friedelin (7), epifriedelanol (8) and β-amyrin (9), a peptide, n-benzoylphen ...201525325562
curcumin improves high glucose-induced ins-1 cell insulin resistance via activation of insulin signaling.curcumin is a yellow pigment isolated from corcuma longan. this research investigates the improvement of curcumin on ins-1 cells with insulin resistance induced by high glucose. ins-1 cells were treated with high glucose (30 mmol l(-1)) for 48 h. subsequently, the medium was replaced with curcumin for 24 h. curcumin effectively increased insulin gene expression and glucose stimulated insulin secretion (gsis) in a dose-dependent manner. furthermore, the molecular mechanism of curcumin-induced ins ...201525474544
effect of microwave power on kinetics and characteristics of microwave vacuum-dried longan (dimocarpus longan lour.) pulp.the drying kinetics of longan (dimocarpus longan lour.) pulp processed by microwave vacuum under different microwave levels (2.67, 5.33, 8.00, and 10.67 w/g) was investigated (pressure controlled at -85 ± 2 kpa) in the present study. it was found that the drying rate of longan pulp was dependent on the microwave power, and the rehydration rate increased from 1.96 to 2.17 with the increase of microwave power from 2.67 to 10.67 w/g. among nine selected thin layer models, the microwave vacuum dryin ...201524367023
activities of different types of thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals.honey is a natural product obtained from the nectar that is collected from flowers by bees. it has several properties, including those of being food and supplementary diet, and it can be used in cosmetic products. honey imparts pharmaceutical properties since it has antibacterial and antioxidant activities. the antibacterial and antioxidant activities of thai honey were investigated in this study.201525654191
endogenous target mimics down-regulate mir160 mediation of arf10, -16, and -17 cleavage during somatic embryogenesis in dimocarpus longan lour.microrna160 plays a critical role in plant development by negatively regulating the auxin response factors arf10, -16, and -17. however, the ways in which mir160 expression is regulated at the transcriptional level, and how mir160 interacts with its targets during plant embryo development, remain unknown. here, we studied the regulatory relationships among endogenous target mimics (etms), and mir160 and its targets, and their involvement in hormone signaling and somatic embryogenesis (se) in dim ...201526594219
water loss from litchi (litchi chinensis) and longan (dimocarpus longan) fruits is biphasic and controlled by a complex pericarpal transpiration barrier.in litchi and longan fruits, a specialised pericarp controls water loss by a protective system consisting of two resistances in series and two water reservoirs separated by a barrier. in the fruits of litchi (litchi chinensis) and longan (dimocarpus longan), the pericarp is solely a protective structure lacking functional stomata and completely enclosing the aril that is the edible part. maintaining a high water content of the fruits is crucial for ensuring the economic value of these important ...201526159434
burst of reactive oxygen species in pedicel-mediated fruit abscission after carbohydrate supply was cut off in longan (dimocarpus longan).cutting off carbohydrate supply to longan (dimocarpus longan lour.) fruit by girdling and defoliation or by detachment induced 100% abscission within a few days. we used these treatments to study the involvement of reactive oxygen species (ros) in fruit abscission. girdling plus defoliation decreased sugar concentrations in the fruit and pedicel and depleted starch grains in the chloroplasts in the cells of abscission zone. prior to the occurrence of intensive fruit abscission, there was a burst ...201526074931
application of α-aminoisobutyric acid and β-aminoisobutyric acid inhibits pericarp browning of harvested longan fruit.pericarp browning is a critical problem resulting in reduced commercial value and shelf life of longan fruit.201526457116
calcium affecting protein expression in longan under simulated acid rain stress.longan (dimocarpus longana lour. cv. wulongling) of uniform one-aged seedlings grown in pots were selected to study specific proteins expressed in leaves under simulated acid rain (siar) stress and exogenous ca(2+) regulation. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) results showed that there was a protein band specifically expressed under siar of ph 2.5 stress for 15 days with its molecular weight of about 23 kd. a 17 kd protein band specifically expressed after siar ...201525893616
isolation and characterization of a catalase gene "hucat3" from pitaya (hylocereus undatus) and its expression under abiotic stress.abiotic stresses usually cause h2o2 accumulation, with harmful effects, in plants. catalase may play a key protective role in plant cells by detoxifying this excess h2o2. pitaya (hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. however, involvement of the pitaya catalase gene (hucat) in tolerance to abiotic stresses is unknown. in the present study, a full-length hucat3 cdna (1870 bp) was isolated from pitaya b ...201525752288
dlran3a is involved in hormone, light, and abiotic stress responses in embryogenic callus of dimocarpus longan lour.ras-related nuclear protein (ran) gtpase plays an important role in nucleo-cytoplasmic transportation of proteins and rna, mitotic spindle assembly, microtubule assembly and nuclear envelope (ne) assembly. we previously identified the full-length cdnas and a dna of dlran3a from longan (dimocarpus longan lour.) somatic embryos and demonstrated its possible roles in cell activities during longan somatic embryogenesis (se). however, thus far little is known of how ran functions in the signaling pat ...201526071187
retardation of lipid oxidation using gelatin film incorporated with longan seed extract compared with bht.the aim of the present work was to apply the gelatin films with different levels of longan seed extract (ls) or butylated hydroxytoluene (bht) on retardation of lipid oxidation in soybean oil. the films incorporated with various concentrations of aqueous ls (0, 50, 100, 300, and 500 ppm) or bht (50, and 100 ppm) were developed. the films had transmittance percentages of 60-80 % at 570 nm and showed good light barrier properties when the concentration of ls or bht increased. about 97 % protein so ...201526344999
biomedical activity and related volatile compounds of thai honeys from 3 different honeybee species.this study investigated the effect of 3 factors (floral source, honeybee species, and postcollection processing) that influence the antibacterial activity, free radical reduction, and other biochemical compositions of different honey types typical of thailand. honey samples from 3 honeybee species (apis mellifera, apis cerana, and apis dorsata) were obtained from 9 floral sources (longan, wild flower, lychee, coffee, sunflower, sesame, bitter bush, para-rubber, and manuka as a control) in differ ...201526317173
a novel macromolecular extract screened from satsuma with pro-inflammatory effect.excessive consumption of horticultural fruit is a double-edged sword with both positive and negative effects. in eastern countries, a large number of people have suffered from shang huo as a result of excessive consumption of "heating" foods, such as lychee, longan, mandarin orange, mango and civet durian. the present study adopted a step by step strategy screened the compositions with pro-inflammatory effect in satsuma fruits. the pro-inflammatory effects of all fractions were evaluated in raw ...201424336758
antimicrobial activities of various fractions of longan (dimocarpus longan lour. fen ke) seed extract.the antimicrobial activities of longan (dimocarpus longan lour. fen ke) seed extracts were investigated using a disc diffusion method and also determining the minimal inhibitory concentration. the dl-p01-si01 fraction showed that the strongest activity against staphylococcus aureus and methicillin-resistant s. aureus at mic 64 μg/ml, which was found to be due to the phenolic compounds. the hplc analysis showed that the major phenolic compounds are gallic acid, corilagin, ethyl gallate and ellagi ...201424533783
effects of encapsulated lactobacillus acidophilus along with pasteurized longan juice on the colon microbiota residing in a dynamic simulator of the human intestinal microbial ecosystem.the effect of encapsulated lactobacillus acidophilus la5 along with pasteurized longan juice on the colon microbiota was investigated by applying a dynamic model of the human gastrointestinal tract. encapsulated l. acidophilus la5 in pasteurized longan juice or sole encapsulated l. acidophilus la5 exhibited the efficiency of colonizing the colon and enabling the growth of colon lactobacilli as well as beneficial bifidobacteria but inhibited the growth of fecal coliforms and clostridia. moreover, ...201423546419
molecular screening and predation evaluation of the key predators of conopomorpha sinensis bradley (lepidoptera: gracilariidae) in litchi orchards.conopomorpha sinensis bradley (lepidoptera: gracilariidae) is one of the major fruit borer pests of litchi and longan in southern china. although chemical control is effective, alternative, biorational strategies are preferable, and should be developed. predators play an important role in the biological control of agricultural pests, but an accurate method for the evaluation of predation in agriculture has not yet been developed. here, we report a new, specific primer pair to amplify a c. sinens ...201424401157
prebiotic activity score and bioactive compounds in longan (dimocarpus longan lour.): influence of pectinase in enzyme-assisted extraction.the optimal extraction of bioactive compounds from longan fruit pulp using pectinex® ultra sp-l pectinase hydrolysis of the fruit homogenate was evaluated. the highest degree of hydrolysis (dh), as determined by the amount of reducing sugars released from the longan pulp, was obtained at a pectinase concentration of 2.5 % (v/w) (257 polygalacturonase units/g fruit) for 4 h. the level of bioactive compounds obtained from the pectinase-treated longan pulp increased with increasing dh to a maximum ...201425190850
antioxidant and α-glucosidase inhibitory activities of 40 tropical juices from malaysia and identification of phenolics from the bioactive fruit juices of barringtonia racemosa and phyllanthus acidus.the present study compared ph, total soluble solids, vitamin c, and total phenolic contents, antioxidant activities, and α-glucosidase inhibitory activities of 40 fresh juices. the juice of baccaurea polyneura showed the highest yield (74.17 ± 1.44%) and total soluble solids (32.83 ± 0.27 °brix). the highest and lowest ph values were respectively measured from the juices of dimocarpus longan (6.87 ± 0.01) and averrhoa bilimbi (1.67 ± 0.67). the juice of psidium guajava gave the highest total phe ...201425198055
effect of extraction and concentration processes on properties of longan syrup.longan (dimocarpus longan lour.) syrup is a novel liquid sweetener produced from longan, one of the traditional and economic fruits in the northern of thailand. in this research, the effect of extraction and concentration processes on properties of longan syrup was investigated. there were two extraction methods (juice extractor and hydraulic press) and three concentration methods (direct heating, steam heating and vacuum evaporation). results overall showed that the extraction method had no sig ...201425190864
determination of gallic acid with rhodanine by reverse flow injection analysis using simplex optimization.a reversed flow injection (rfi) system was designed and constructed for gallic acid determination. gallic acid was determined based on the formation of chromogen between gallic acid and rhodanine, resulting in a colored product with a λmax at 520 nm. the optimum conditions for determining gallic acid were also investigated. optimizations of the experimental conditions were carried out based on the so-call univariate method. the conditions obtained were 0.6% (w/v) rhodanine, 70% (v/v) ethanol, 0. ...201425159449
determination of rice syrup adulterant concentration in honey using three-dimensional fluorescence spectra and multivariate calibrations.to rapidly and efficiently detect the presence of adulterants in honey, three-dimensional fluorescence spectroscopy (3dfs) technique was employed with the help of multivariate calibration. the data of 3d fluorescence spectra were compressed using characteristic extraction and the principal component analysis (pca). then, partial least squares (pls) and back propagation neural network (bp-ann) algorithms were used for modeling. the model was optimized by cross validation, and its performance was ...201424830631
antitumor activity of a polysaccharide from longan seed on lung cancer cell line a549 in vitro and in vivo.in this study, a water-soluble longan seed polysaccharide (wlsp), with a molecular weight of 57 kda, was isolated from longan seed. gas chromatography (gc) analysis showed that wlsp was composed mainly of rhamnose (rha), mannose (man), arabinose (ara), galactose (gal), and glucose (glc), with molar ratios of 2.4:1.5:2.3:5.6:6.5. the result in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay showed that wlsp showed a dose-dependent antiproliferative effect on the prolifera ...201424777333
ameliorating effects of hx106n, a water-soluble botanical formulation, on aβ25-35-induced memory impairment and oxidative stress in mice.it is well-established that amyloid β (aβ)-induced oxidative stress plays a crucial role in alzheimer's disease (ad) and its cognitive deficits. hx106n is a water-soluble extract prepared from a mixture of the plants dimocarpus longan, liriope platyphylla, salvia miltiorrhiza, and gastrodia elata. these ingredients are traditionally used in various plant-based medicines for the treatment of neurological disease. in this study, we examined the effects of hx106n on memory impairment and oxidative ...201424694662
sulfated modification of longan polysaccharide and its immunomodulatory and antitumor activity in vitro.a water-soluble polysaccharide fraction (lp1) was prepared from dimocarpus longan lour. by hot water extraction, deae-cellulose and sephadex g-100 chromatography. its sulfated derivative (lp1-s) was prepared by the sulfuric acid method. preliminary tests in vitro showed lp1 and lp1-s could stimulate murine lymphocytes proliferation, increase pinocytic activity of murine macrophages and production of nitric oxide (no), interleukin 6 (il-6), il-1β and tumor necrosis factor-alpha (tnf-α) in macroph ...201424680807
cloning and molecular characterization of phospholipase d (pld) delta gene from longan (dimocarpus longan lour.).longan (dimocarpus longan lour.) is a non-climacteric fruit with a short postharvest life. the regulation of phospholipase d (pld) activity closely relates to postharvest browning and senescence of longan fruit. in this study, a novel cdna clone of longan pldδ (lgpldδ) was obtained and registered in genbank (accession no. jf791814). the deduced amino acid sequence possessed all of the three typical domains of plant plds, a c2 domain and two catalytic hxkxxxxd motifs. the tertiary structure of lg ...201424590739
screening of key antioxidant compounds of longan (dimocarpus longan lour.) seed extract by combining online fishing/knockout, activity evaluation, fourier transform ion cyclotron resonance mass spectrometry, and high-performance liquid chromatography electrospray ionization mass spectrometry methods.to figure out the key phenolic compounds accounting for the antioxidant effects of longan (dimocarpus longan lour.) seed extract, online fishing/knockout method, activity evaluation assays, fourier transform ion cyclotron resonance mass spectrometry (ft-icr-ms), and high-performance liquid chromatography electrospray ionization mass spectrometry (hplc-esi-ms) analysis were used jointly for the first time. p-coumaric acid-glycoside, (s)-flavogallonic acid, ellagic acid derivative, and methyl-ella ...201425220361
structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from dimocarpus longan pulp.a new water-soluble polysaccharide (longan polysaccharide 1 (lp1)) was extracted and successfully purified from dimocarpus longan pulp via diethylaminoethyl (deae)-cellulose anion-exchange and sephacryl s-300 hr gel chromatography. the chemical structure was determined using infrared (ir), gas chromatography (gc) and nuclear magnetic resonance (nmr) analysis. the results indicated that the molecular weight of the sample was 1.1 × 10(5) da. monosaccharide composition analysis revealed that lp1 wa ...201424663085
genetic characterization and authentication of dimocarpus longan lour. using an improved rapd technique.dimocarpus longan lour. is an edible and traditional herb in china, commonly referred to as longon. an improved randomly amplified polymorphic dna (rapd) protocol was here developed in order to determine the geographical origins of d. longan samples collected from 5 provinces in the southern and southwestern areas of china, including sichuan, hainan, fujian, guangdong, and guangxi. generally, the improved rapd method generated good fingerprinting of the 5 samples using the selected 17 primers. i ...201424634243
identification of regulatory genes implicated in continuous flowering of longan (dimocarpus longan l.).longan (dimocarpus longan l.) is a tropical/subtropical fruit tree of significant economic importance in southeast asia. however, a lack of transcriptomic and genomic information hinders research on longan traits, such as the control of flowering. in this study, high-throughput rna sequencing (rna-seq) was used to investigate differentially expressed genes between a unique longan cultivar 'sijimi'(s) which flowers throughout the year and a more typical cultivar 'lidongben'(l) which flowers only ...201425479005
inhibitory effects of dried longan (euphoria longana lam.) seed extract on invasion and matrix metalloproteinases of colon cancer cells.the critical step in colorectal cancer progression and associated mortality is cancer invasion, which depends on two key gelatinase enzymes, matrix metalloproteinases-2 and -9. dried longan ( euphoria longana lam.) seed is a rich natural source of antioxidant polyphenols.this study evaluated the effect of dried longan seeds on colon cancer cell invasion via gelatinase function and expression. three dried longan seed fractions were collected by sephadex lh-20 column chromatography. they showed a ...201323527961
evaluation of suitable reference genes for normalization of microrna expression by real-time reverse transcription pcr analysis during longan somatic embryogenesis.accurate profiling of micrornas (mirnas) is an essential step for understanding both developmental and physiological functions of mirnas. real-time quantitative pcr (qpcr) is being widely used in mirna expression studies, but choosing a suitable reference gene is a crucial factor for correct analysis of results. to date, there has been no systematic evaluation of qpcr reference genes for the study of mirnas during somatic embryogenesis (se) in the longan tree (dimocarpus longan). here, the most ...201323454294
inhibitory effects of propyl gallate on tyrosinase and its application in controlling pericarp browning of harvested longan fruits.tyrosinase (ec 1.14.18.1), also known as polyphenol oxidase (ppo), is a key enzyme in pigment biosynthesis of organisms. the inhibitory effects of propyl gallate on the activity of mushroom tyrosinase and effects of propyl gallate on pericarp browning of harvested longan fruits in relation to phenolic metabolism were investigated. the results showed that propyl gallate could potently inhibit diphenolase activity of tyrosinase. the inhibitor concentration leading to 50% activity lost (ic50) was d ...201323427826
structural characteristics and antioxidant activities of polysaccharides from longan seed.ultrasound-assisted extraction was employed to extract polysaccharides from longan seed (lsp), with aids of a box-behnken statistical design to investigate the effects of ultrasonic power, time and liquid/solid ratio on the extraction recovery of the lsp. the structural analysis indicated that arabinose, galactose, glucose and mannose were major components of lsp, with →6)-gal-(→1, glc-(→1 and →6)-glc-(1→ glycosidic linkages. in an in vitro antioxidant activity of the 1,1-diphenyl-2-picryldydraz ...201323218364
comparative analysis reveals dynamic changes in mirnas and their targets and expression during somatic embryogenesis in longan (dimocarpus longan lour.).somatic embryogenesis (se), which resembles zygotic embryogenesis, is an essential component of the process of plant cell differentiation and embryo development. although micrornas (mirnas) are important regulators of many plant develop- mental processes, their roles in se have not been thoroughly investigated. in this study, we used deep-sequencing, computational, and qpcr methods to identify, profile, and describe conserved and novel mirnas involved in longan (dimocarpus longan) se. a total of ...201323593197
structural identification of (1→6)-α-d-glucan, a key responsible for the health benefits of longan, and evaluation of anticancer activity.longan is a delicious subtropical fruit with great health-beneficial effects. it has been utilized for disease prevention and health care since ancient age. to explore the chemicals responsible for the health benefits, water-soluble polysaccharides were extracted from longan flesh in this work. a pure polysaccharide (lps1) was obtained through column purification. analysis by gas chromatography showed lps1 was a homopolysaccharide of glucose with glycosidic linkage of →6)-d-glc-(1→. nuclear magn ...201323617585
[effects of exogenous nitric oxide on physiological characteristics of longan (dimocarpus longana) seedlings under acid rain stress].this paper studied the effects of exogenous nitric oxide donor sodium nitroprusside (snp) on the chlorophyll content, antioxidant enzyme activities, and osmotic regulation substances of longan (dimocarpus longana 'fuyan') seedlings under acid rain (ph 3.0) stress. under the acid rain stress, the seedling leaf superoxide dismutase (sod), peroxidase (pod) and catalase (cat) activities and chlorophyll, soluble protein and soluble sugar contents decreased obviously, while the leaf malondialdedyde co ...201324380343
modification of pectin and hemicellulose polysaccharides in relation to aril breakdown of harvested longan fruit.to investigate the modification of cell wall polysaccharides in relation to aril breakdown in harvested longan fruit, three pectin fractions (wsp, water soluble pectin; csp, cdta-soluble pectin; asp, alkali soluble pectin) and one hemicellulose fraction (4 m koh-shc, 4 m koh-soluble hemicellulose) were extracted, and their contents, monosaccharide compositions and molecular weights were evaluated. as aril breakdown intensified, csp content increased while asp and 4 m koh-shc contents decreased, ...201324287911
molecular cloning and development of rapd-scar markers for dimocarpus longan variety authentication.as an edible fruit and source of traditional medicine, d. longan is grown in most areas of southern china. identification of d. longan cultivars by using molecular markers is important genetically. in this study, we cloned fragments from improved randomly amplified polymorphic dna (rapd), and developed stably diagnostic sequence-characterized amplified region (scar) markers. the specific rapd bands of d. longan cultivars from guangxi, with size ranging from 500 bp to 900 bp were gel-purified, cl ...201324130961
metal-organic framework-199/graphite oxide hybrid composites coated solid-phase microextraction fibers coupled with gas chromatography for determination of organochlorine pesticides from complicated samples.the hybrid material of a copper-based metal-organic framework (mof-199) and graphite oxide (go) was explored as the solid-phase microextraction (spme) coating for the first time. this fiber was fabricated by using 3-amino-propyltriethoxysilane (aptes) as the cross-linking agent, which enhanced its durability and allowed more than 140 replicate extractions. with the incorporation of go, the mof-199/go fibers with go contents ranging from 5 to 15 wt% exhibited enhanced adsorption affinity to organ ...201324054558
solution properties and in vitro anti-tumor activities of polysaccharides from longan pulp.the solution properties of four fractions (lpi-iv) from crude longan pulp polysaccharides (lp3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with congo red, and atomic force microscopy. their radii of gyration <s²>(z)(½) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <s²>(z)(½) = k m(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 ml/g, respectively. moreover, the depen ...201324051475
analysis of the global transcriptome of longan (dimocarpus longan lour.) embryogenic callus using illumina paired-end sequencing.longan is a tropical/subtropical fruit tree of great economic importance in southeast asia. progress in understanding molecular mechanisms of longan embryogenesis, which is the primary influence on fruit quality and yield, is slowed by lack of transcriptomic and genomic information. illumina second generation sequencing, which is suitable for generating enormous numbers of transcript sequences that can be used for functional genomic analysis of longan.201323957614
isolation and characterization of flowering locus t subforms and apetala1 of the subtropical fruit tree dimocarpus longan.longan (dimocarpus longan lour.) is a subtropical evergreen fruit tree, mainly cultivated in asia. two putative floral integrator genes, d. longan flowering locus t1 and 2 (dlft1 and dlft2) were isolated and both translated sequences revealed a high homology to ft sequences from other plants. moreover, two apetala1-like (dlap1-1 and dlap1-2) sequences from longan were isolated and characterized. results indicate that the sequences of these genes are highly conserved, suggesting functions in the ...201323954797
efficacy of polymer coating of probiotic beads suspended in pressurized and pasteurized longan juices on the exposure to simulated gastrointestinal environment.alginate-coated lactobacillus acidophilus la5 or lactobacillus casei 01 was recoated with either 0.1-0.5% (w/v) alginate or 0.05-0.15% (w/v) poly-l-lysine (pll) plus 0.2% (w/v) alginate or 5-15% (w/v) gelatin, after which they were determined for survivability in gastric or bile longan juices. the morphology of encapsulated probiotic cells illustrated that recoated beads with 0.5% alginate showed a more compact surface and a greater protective effect than other recoating materials. the recoated ...201323701113
identification of a ptc-containing dlran transcript and its differential expression during somatic embryogenesis in dimocarpus longan.ran (ras-related nuclear protein) plays crucial roles in multiple cellular processes in yeast, animals and plants. here we present a dlran gene and its alternative splicing transcripts containing premature terminator codons (ptcs), identified from embryogenic cultures in longan. multiple alignment and splicing pattern analyses indicated that dlran-1 transcript harboring ptc was the consequence of alternative splicing. the accumulation of dlran ptc-containing transcripts increased significantly w ...201323933275
activities of free and encapsulated lactobacillus acidophilus la5 or lactobacillus casei 01 in processed longan juices on exposure to simulated gastrointestinal tract.fruit drinks containing probiotics are gaining interest in the global marketplace. for example, longan juice, containing carbohydrate and various bioactive components, is a potentially health-promoting beverage as well as probiotic carrier for human consumption. in this study, high-pressure and thermal processes were applied to eliminate competitive micro-organisms in longan juice prior to the addition of lactobacillus acidophilus la5 or lactobacillus casei 01. the activities of these probiotics ...201323401169
effect of floral sources on the antioxidant, antimicrobial, and anti-inflammatory activities of honeys in taiwan.we evaluated the antioxidant, antibacterial, and anti-inflammatory activities of honey made from different floral sources, including the medicinal herb bidens pilosa, fruit trees, dimocarpus longan, litchi chinensis, and citrus maxima, the taiwanese endemic plant aglaia formosana, and a multifloral forest. the total phenolic and flavonoid contents of the honey made from b. pilosa were significantly higher than those of the other honeys. the honey from b. pilosa also had significantly greater sca ...201323561193
evaluation of physicochemical and antioxidant properties of sourwood and other malaysian honeys: a comparison with manuka honey.the aim of the present study was to evaluate the physical, biochemical and antioxidant properties of four malaysian monofloral types of honey (gelam, longan, rubber tree and sourwood honeys) compared to manuka honey. several physical parameters of honey, such as ph, moisture content, electrical conductivity (ec), total dissolved solids (tds), color intensity, total sugar and sucrose content, were measured. a number of biochemical and antioxidant tests were performed to determine the antioxidant ...201323938192
in vitro antifungal activities of longan (dimocarpus longan lour.) seed extract.longan, dimocarpus longan lour., contains polyphenolic compounds which exhibit several pharmacological properties. this study aims to evaluate antifungal activities of longan fruit extract in comparison to its active compounds. the results showed that longan seed exhibited antifungal activity against the opportunistic yeasts (candida species and cryptococcus neoformans). in contrast, longan pulp and whole fruit did not demonstrate any inhibitory effects. ellagic acid showed the most potent antif ...201222245574
Histone deacetylase HD2 interacts with ERF1 and is involved in longan fruit senescence.Histone deacetylation plays an important role in epigenetic control of gene expression. HD2 is a plant-specific histone deacetylase that is able to mediate transcriptional repression in many biological processes. To investigate the epigenetic and transcriptional mechanisms of longan fruit senescence, one histone deacetylase 2-like gene, DlHD2, and two ethylene-responsive factor-like genes, DlERF1 and DlERF2, were cloned and characterized from longan fruit. Expression of these genes was examined ...201221926091
morphology and volatile compounds of metathoracic scent gland in tessaratoma papillosa (drury) (hemiptera: tessaratomidae).tessaratoma papillosa (drury) (hemiptera: tessaratomidae) is a serious insect pest of litchi and longan in south china. when disturbed, this insect could release large quantities of disagreeable odorous volatiles from its scent gland. knowledge on the scent gland and its secretion is crucial for developing the semiochemical methods to manage this pest. morphology and ultrastructure of the metathoracic scent glands (mtgs) were studied under stereo and scanning electron microscopy, and the volatil ...201223950062
inhibitory effects of water extract from longan twigs on mutation and nitric oxide production.this study examines the inhibitory effects of water extract from longan twigs (wlts) on mutation and nitric oxide (no) production. the results show that wlt inhibited the mutagenicity of 2-aminoanthracene (2-aa), an indirect mutagen, and 4-nitroquinoline-n-oxide (4-nqo), a direct mutagen toward salmonella typhimurium ta 98 and ta 100. in addition, wlt in the range 0-0.6 mg/ml showed radical scavenging, reducing activities and chelating activity, as well as decreased lipid oxidative damage. meanw ...201222868111
isolation and characterization of ellagitannins as the major polyphenolic components of longan (dimocarpus longan lour) seeds.longan (dimocarpus longan lour, syn. euphoria longan lam.) represents an important fruit in northern thailand and has significant economic impact. the fruit is either consumed fresh or as commercially prepared dried and canned products. the canning industry in thailand produces considerable quantities of waste products, in particular longan seeds. because these seeds may be an exploitable source of natural phenolic antioxidants, it was of interest to identify, purify and quantitate the major pot ...201222277734
floral reversion mechanism in longan (dimocarpus longan lour.) revealed by proteomic and anatomic analyses.two-dimensional gel electrophoresis (2-de) was used to analyze the proteins related to floral reversion in dimocarpus longan lour. proteins were extracted from buds undergoing the normal process of flowering and from those undergoing floral reversion in three developing stages in d. longan. differentially expressed proteins were identified from the gels after 2-de analysis, which were confirmed using matrix-assisted laser desorption/ionization-time of flying-mass spectroscopy and protein databas ...201222100459
comparison of dynamic viscoelastic and physicochemical properties of pressurised and pasteurised longan juices with xanthan addition.physical and biochemical properties of pressurised and pasteurised longan juices with various xanthan additions, such as viscoelastic behaviour, colour l (lightness), -a(*) (greenness), b(*) (yellowness), δe (total different colours) and bi (browning index) parameters, polyphenol oxidase (ppo) activity, ascorbic acid, gallic acid, ellagic acid, total phenols and antioxidant capacity (dpph assay) were studied. viscoelastic determination indicated that longan juice with 0.15% xanthan addition was ...201223442674
antioxidant and anti-inflammatory properties of longan (dimocarpus longan lour.) pericarp.this study examined the antioxidant and anti-inflammatory activities of the water extract of longan pericarp (wlp). the results showed that wlp exhibited radical scavenging, reducing activity and liposome protection activity. in addition, wlp also inhibited lipopolysaccharide (lps)-induced nitric oxide (no) production in macrophages. further, administration of wlp, in the range of 100-400 mg/kg, showed a concentration-dependent inhibition on paw edema development following carrageenan (carr) tre ...201222966244
longan seed extract reduces hyperuricemia via modulating urate transporters and suppressing xanthine oxidase activity.hyperuricemia causes gouty arthritis, kidney disease, heart disease, and other diseases. xanthine oxidase (xod) and urate transporters play important roles in urate homeostasis. numerous plants have been identified as xod inhibitors. longan seeds are known to contain high levels of polyphenols such as corilagin, gallic acid and ellagic acid. we examined the effect of longan seed extract on xod inhibition and urate transporters glut1 and glut9 using both in vitro and in vivo assays. the results s ...201222928829
neuroprotective effects of longan ( dimocarpus longan lour.) flower water extract on mpp+-induced neurotoxicity in rat brain.in this study, the neuroprotective effect of dimocarpus longan lour. flower water extract (lfwe) was investigated. first, an in vitro study showed that lfwe concentration-dependently inhibited lipid peroxidation of brain homogenates incubated at 37 °c. the antioxidative activity of lfwe was more potent than that of glutathione or trolox. furthermore, an ex vivo study found that the basal lipid peroxidation (0 °c) and lipid peroxidation incubated at 37 °c were lower in the brain homogenates of lf ...201222920583
safety evaluation of longan seed extract: acute and repeated oral administration.longan seed extract (lse) contains high levels of beneficial polyphenolic compounds. the present study evaluated acute and repeated dose (4 and 13 weeks) toxicological effects of aqueous extract of longan seed to ensure the safety of utilization of this extract. our study revealed that all treated animals survived through the whole experimental periods without adverse effects observed in either sex of animals after acute and repeated dose (4 and 13 weeks) oral administration of lse. likewise, gr ...201222902805
potential roles of longan flower and seed extracts for anti-cancer.polyphenol-rich plants are known to possess benefits to human health. recent studies have revealed that many traditional chinese medicines (tcms) are rich sources of polyphenols and exhibit antioxidant and anti-inflammatory activities, and these tcms have been shown experimentally to overcome some chronic diseases, including cancer. longan flowers and seeds, two tcms traditionally used for relieving pain and urinary diseases, have been revealed in our recent reports and other studies to possess ...201224520538
a new natural lactone from dimocarpus longan lour. seeds.a new natural product named longanlactone was isolated from dimocarpus longan lour. seeds. its structure was determined as 3-(2-acetyl-1h--pyrrol-1-yl)-5-(prop-2-yn-1-yl)dihydrofuran-2(3h)-one by spectroscopic methods and hresims.201222869162
role of hydroxyl radical in modification of cell wall polysaccharides and aril breakdown during senescence of harvested longan fruit.the effects of reactive oxygen species (ros), especially hydroxyl radical (oh), on cellular wall disassembly in vitro and in vivo, and aril breakdown in longan fruit, were investigated. oh caused the disassembly of cell wall material (cwm) in vitro, demonstrated by the increases of total sugars and uronic acid released, and the downshift in molecular mass of cdta soluble pectin (csp) and na2co3 soluble pectin (co3-sp). in addition, oh resulted in decreased co3-sp content and increased csp conten ...201125214349
physicochemical characteristics and immunomodulatory activities of three polysaccharide-protein complexes of longan pulp.three polysaccharide-protein complexes of longan pulp (lp1-3) were isolated in this work. their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (nk) cells and macrophages in vitro were investigated. the carbohydrate portions of lp1-3 were principally composed of glucose, arabinose and mannose. lp3 displayed the maximal moisture absorption, and the thermal stability of lp2 was obviously higher than that of lp1 and lp3. all of them showed the characteris ...201121778950
Refresh
firstprev
12
nextlast
Displaying items 1 - 100 of 159