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ameliorating effects of hx106n, a water-soluble botanical formulation, on aβ25-35-induced memory impairment and oxidative stress in mice.it is well-established that amyloid β (aβ)-induced oxidative stress plays a crucial role in alzheimer's disease (ad) and its cognitive deficits. hx106n is a water-soluble extract prepared from a mixture of the plants dimocarpus longan, liriope platyphylla, salvia miltiorrhiza, and gastrodia elata. these ingredients are traditionally used in various plant-based medicines for the treatment of neurological disease. in this study, we examined the effects of hx106n on memory impairment and oxidative ...201424694662
antitumor activity of a polysaccharide from longan seed on lung cancer cell line a549 in vitro and in vivo.in this study, a water-soluble longan seed polysaccharide (wlsp), with a molecular weight of 57 kda, was isolated from longan seed. gas chromatography (gc) analysis showed that wlsp was composed mainly of rhamnose (rha), mannose (man), arabinose (ara), galactose (gal), and glucose (glc), with molar ratios of 2.4:1.5:2.3:5.6:6.5. the result in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay showed that wlsp showed a dose-dependent antiproliferative effect on the prolifera ...201424777333
determination of rice syrup adulterant concentration in honey using three-dimensional fluorescence spectra and multivariate calibrations.to rapidly and efficiently detect the presence of adulterants in honey, three-dimensional fluorescence spectroscopy (3dfs) technique was employed with the help of multivariate calibration. the data of 3d fluorescence spectra were compressed using characteristic extraction and the principal component analysis (pca). then, partial least squares (pls) and back propagation neural network (bp-ann) algorithms were used for modeling. the model was optimized by cross validation, and its performance was ...201424830631
determination of gallic acid with rhodanine by reverse flow injection analysis using simplex optimization.a reversed flow injection (rfi) system was designed and constructed for gallic acid determination. gallic acid was determined based on the formation of chromogen between gallic acid and rhodanine, resulting in a colored product with a λmax at 520 nm. the optimum conditions for determining gallic acid were also investigated. optimizations of the experimental conditions were carried out based on the so-call univariate method. the conditions obtained were 0.6% (w/v) rhodanine, 70% (v/v) ethanol, 0. ...201425159449
prebiotic activity score and bioactive compounds in longan (dimocarpus longan lour.): influence of pectinase in enzyme-assisted extraction.the optimal extraction of bioactive compounds from longan fruit pulp using pectinex® ultra sp-l pectinase hydrolysis of the fruit homogenate was evaluated. the highest degree of hydrolysis (dh), as determined by the amount of reducing sugars released from the longan pulp, was obtained at a pectinase concentration of 2.5 % (v/w) (257 polygalacturonase units/g fruit) for 4 h. the level of bioactive compounds obtained from the pectinase-treated longan pulp increased with increasing dh to a maximum ...201425190850
effect of extraction and concentration processes on properties of longan syrup.longan (dimocarpus longan lour.) syrup is a novel liquid sweetener produced from longan, one of the traditional and economic fruits in the northern of thailand. in this research, the effect of extraction and concentration processes on properties of longan syrup was investigated. there were two extraction methods (juice extractor and hydraulic press) and three concentration methods (direct heating, steam heating and vacuum evaporation). results overall showed that the extraction method had no sig ...201425190864
antioxidant and α-glucosidase inhibitory activities of 40 tropical juices from malaysia and identification of phenolics from the bioactive fruit juices of barringtonia racemosa and phyllanthus acidus.the present study compared ph, total soluble solids, vitamin c, and total phenolic contents, antioxidant activities, and α-glucosidase inhibitory activities of 40 fresh juices. the juice of baccaurea polyneura showed the highest yield (74.17 ± 1.44%) and total soluble solids (32.83 ± 0.27 °brix). the highest and lowest ph values were respectively measured from the juices of dimocarpus longan (6.87 ± 0.01) and averrhoa bilimbi (1.67 ± 0.67). the juice of psidium guajava gave the highest total phe ...201425198055
role of hydroxyl radical in modification of cell wall polysaccharides and aril breakdown during senescence of harvested longan fruit.the effects of reactive oxygen species (ros), especially hydroxyl radical (oh), on cellular wall disassembly in vitro and in vivo, and aril breakdown in longan fruit, were investigated. oh caused the disassembly of cell wall material (cwm) in vitro, demonstrated by the increases of total sugars and uronic acid released, and the downshift in molecular mass of cdta soluble pectin (csp) and na2co3 soluble pectin (co3-sp). in addition, oh resulted in decreased co3-sp content and increased csp conten ...201125214349
screening of key antioxidant compounds of longan (dimocarpus longan lour.) seed extract by combining online fishing/knockout, activity evaluation, fourier transform ion cyclotron resonance mass spectrometry, and high-performance liquid chromatography electrospray ionization mass spectrometry methods.to figure out the key phenolic compounds accounting for the antioxidant effects of longan (dimocarpus longan lour.) seed extract, online fishing/knockout method, activity evaluation assays, fourier transform ion cyclotron resonance mass spectrometry (ft-icr-ms), and high-performance liquid chromatography electrospray ionization mass spectrometry (hplc-esi-ms) analysis were used jointly for the first time. p-coumaric acid-glycoside, (s)-flavogallonic acid, ellagic acid derivative, and methyl-ella ...201425220361
two new flavonol glycosides from dimocarpus longan leaves.from the extracts of dimocarpus longan lour leaves, 2 unusual flavonol glycosides, quercetin 3-o-(3″-o-2‴-methyl-2‴-hydroxylethyl)-β-d-xyloside (1) and quercetin 3-o-(3″-o-2‴-methyl-2‴-hydroxylethyl)-α-l-rhamnopyranoside (2), as well as 10 known compounds including 2 flavonol glycosides, afzelin (3) and kaempferol-3-o-α-l-rhamnopyranoside (4), 2 flavans, (-)-epicatechin (5) and proanthocyanidin a-2 (6), 3 triterpenoids, friedelin (7), epifriedelanol (8) and β-amyrin (9), a peptide, n-benzoylphen ...201525325562
curcumin improves high glucose-induced ins-1 cell insulin resistance via activation of insulin signaling.curcumin is a yellow pigment isolated from corcuma longan. this research investigates the improvement of curcumin on ins-1 cells with insulin resistance induced by high glucose. ins-1 cells were treated with high glucose (30 mmol l(-1)) for 48 h. subsequently, the medium was replaced with curcumin for 24 h. curcumin effectively increased insulin gene expression and glucose stimulated insulin secretion (gsis) in a dose-dependent manner. furthermore, the molecular mechanism of curcumin-induced ins ...201525474544
identification of regulatory genes implicated in continuous flowering of longan (dimocarpus longan l.).longan (dimocarpus longan l.) is a tropical/subtropical fruit tree of significant economic importance in southeast asia. however, a lack of transcriptomic and genomic information hinders research on longan traits, such as the control of flowering. in this study, high-throughput rna sequencing (rna-seq) was used to investigate differentially expressed genes between a unique longan cultivar 'sijimi'(s) which flowers throughout the year and a more typical cultivar 'lidongben'(l) which flowers only ...201425479005
activities of different types of thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals.honey is a natural product obtained from the nectar that is collected from flowers by bees. it has several properties, including those of being food and supplementary diet, and it can be used in cosmetic products. honey imparts pharmaceutical properties since it has antibacterial and antioxidant activities. the antibacterial and antioxidant activities of thai honey were investigated in this study.201525654191
isolation and characterization of a catalase gene "hucat3" from pitaya (hylocereus undatus) and its expression under abiotic stress.abiotic stresses usually cause h2o2 accumulation, with harmful effects, in plants. catalase may play a key protective role in plant cells by detoxifying this excess h2o2. pitaya (hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. however, involvement of the pitaya catalase gene (hucat) in tolerance to abiotic stresses is unknown. in the present study, a full-length hucat3 cdna (1870 bp) was isolated from pitaya b ...201525752288
calcium affecting protein expression in longan under simulated acid rain stress.longan (dimocarpus longana lour. cv. wulongling) of uniform one-aged seedlings grown in pots were selected to study specific proteins expressed in leaves under simulated acid rain (siar) stress and exogenous ca(2+) regulation. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) results showed that there was a protein band specifically expressed under siar of ph 2.5 stress for 15 days with its molecular weight of about 23 kd. a 17 kd protein band specifically expressed after siar ...201525893616
optimization of tyrosinase inhibition activity of ultrasonic-extracted polysaccharides from longan fruit pericarp.various ultrasonic conditions were employed to prepare polysaccharides from longan fruit pericarp (plfp) and the lineweaver-burk equation was then used to determine the effect of plfp on inhibition of tyrosinase activity. this result showed that plfp acted as a non-competitive inhibitor of tyrosinase. the highest slope was observed for ultrasonic extraction, followed by the hot-water extraction, suggesting that the ultrasonic treatment of plfp increased the inhibition of tyrosinase activity. fur ...200826049219
dlran3a is involved in hormone, light, and abiotic stress responses in embryogenic callus of dimocarpus longan lour.ras-related nuclear protein (ran) gtpase plays an important role in nucleo-cytoplasmic transportation of proteins and rna, mitotic spindle assembly, microtubule assembly and nuclear envelope (ne) assembly. we previously identified the full-length cdnas and a dna of dlran3a from longan (dimocarpus longan lour.) somatic embryos and demonstrated its possible roles in cell activities during longan somatic embryogenesis (se). however, thus far little is known of how ran functions in the signaling pat ...201526071187
burst of reactive oxygen species in pedicel-mediated fruit abscission after carbohydrate supply was cut off in longan (dimocarpus longan).cutting off carbohydrate supply to longan (dimocarpus longan lour.) fruit by girdling and defoliation or by detachment induced 100% abscission within a few days. we used these treatments to study the involvement of reactive oxygen species (ros) in fruit abscission. girdling plus defoliation decreased sugar concentrations in the fruit and pedicel and depleted starch grains in the chloroplasts in the cells of abscission zone. prior to the occurrence of intensive fruit abscission, there was a burst ...201526074931
identification and characterisation of two general odourant-binding proteins from the litchi fruit borer, conopomorpha sinensis bradley.the litchi fruit borer, conopomorpha sinensis bradley, is one of the most destructive pests of litchi and longan fruits in south-east asia and southern china, yet the molecular biology and physiology of this pest remain poorly understood. control of this insect pest may be achieved by interfering with its recognition of host plants.201626085035
water loss from litchi (litchi chinensis) and longan (dimocarpus longan) fruits is biphasic and controlled by a complex pericarpal transpiration barrier.in litchi and longan fruits, a specialised pericarp controls water loss by a protective system consisting of two resistances in series and two water reservoirs separated by a barrier. in the fruits of litchi (litchi chinensis) and longan (dimocarpus longan), the pericarp is solely a protective structure lacking functional stomata and completely enclosing the aril that is the edible part. maintaining a high water content of the fruits is crucial for ensuring the economic value of these important ...201526159434
effect of longan polysaccharides on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro.for autologous chondrocyte implantation (aci) to restore cartilage defect, limited cell numbers and dedifferentiation of chondrocytes are the major difficulties. an alternative is the use of growth factors, but the high cost and potential tumorigenesis are the major obstacles. to ensure successful aci therapy, it is of significance to find effective substituted pro-chondrogenic agent. polysaccharides from plant extract have low toxicity and few undesirable side effects, which were reported to fa ...201626231088
anti-hepatitis c virus activity of a crude extract from longan (dimocarpus longan lour.) leaves.infection with hepatitis c virus (hcv) results in hepatitis c, a disease characterized by chronic infection, cirrhosis, and hepatocellular carcinoma. currently, the standard therapy is a combination of pegylated interferon-α plus ribavirin with ns3 protease inhibitors. addition of ns3 protease inhibitors to the standard therapy improves response rates; however, use of ns3 protease inhibitors is also associated with significant adverse effects and an increase in the overall cost of treatment. the ...201626255732
biomedical activity and related volatile compounds of thai honeys from 3 different honeybee species.this study investigated the effect of 3 factors (floral source, honeybee species, and postcollection processing) that influence the antibacterial activity, free radical reduction, and other biochemical compositions of different honey types typical of thailand. honey samples from 3 honeybee species (apis mellifera, apis cerana, and apis dorsata) were obtained from 9 floral sources (longan, wild flower, lychee, coffee, sunflower, sesame, bitter bush, para-rubber, and manuka as a control) in differ ...201526317173
retardation of lipid oxidation using gelatin film incorporated with longan seed extract compared with bht.the aim of the present work was to apply the gelatin films with different levels of longan seed extract (ls) or butylated hydroxytoluene (bht) on retardation of lipid oxidation in soybean oil. the films incorporated with various concentrations of aqueous ls (0, 50, 100, 300, and 500 ppm) or bht (50, and 100 ppm) were developed. the films had transmittance percentages of 60-80 % at 570 nm and showed good light barrier properties when the concentration of ls or bht increased. about 97 % protein so ...201526344999
application of α-aminoisobutyric acid and β-aminoisobutyric acid inhibits pericarp browning of harvested longan fruit.pericarp browning is a critical problem resulting in reduced commercial value and shelf life of longan fruit.201526457116
advances on bioactive polysaccharides from medicinal plants.in recent decades, the polysaccharides from the medicinal plants have attracted a lot of attention due to their significant bioactivities, such as anti-tumor activity, antioxidant activity, anticoagulant activity, antidiabetic activity, radioprotection effect, anti-viral activity, hypolipidemic and immunomodulatory activities, which make them suitable for medicinal applications. previous studies have also shown that medicinal plant polysaccharides are non-toxic and show no side effects. based on ...201626463231
identification of monofloral honeys using hplc-ecd and chemometrics.a total of 77 jujube, longan and chaste honey samples were collected from 18 different areas of china. thirteen types of phenolic acids in the honey samples were analysed using high-performance liquid chromatography with electrochemical detection (hplc-ecd). moreover, hplc-ecd fingerprints of the monofloral honey samples were established. from the analysis of the hplc-ecd fingerprints, common chromatography peak information was obtained, and principal component analysis and discriminant analysis ...201626471540
microanalysis, pharmacokinetics and tissue distribution of polysaccharide-protein complexes from longan pulp in mice.a high performance size exclusion-fluorescence detection (hpsec-fd) method combined with fluorescein isothiocyanate (fitc) prelabeling was established for the microanalysis of polysaccharide-protein complexes from longan pulp (lpp). fitc-labeled lpp (lppf) was fractionated by gel filtration chromatography. the weight-average molecular weight and fitc substitution degree of lppf were 39.01 kda and 0.20%, respectively. the hpsec-fd calibration curves linear over the range of 1-200 µg/ml in mouse p ...201526501257
developing single nucleotide polymorphism (snp) markers from transcriptome sequences for identification of longan (dimocarpus longan) germplasm.longan (dimocarpus longan lour.) is an important tropical fruit tree crop. accurate varietal identification is essential for germplasm management and breeding. using longan transcriptome sequences from public databases, we developed single nucleotide polymorphism (snp) markers; validated 60 snps in 50 longan germplasm accessions, including cultivated varieties and wild germplasm; and designated 25 snp markers that unambiguously identified all tested longan varieties with high statistical rigor ( ...201526504559
endogenous target mimics down-regulate mir160 mediation of arf10, -16, and -17 cleavage during somatic embryogenesis in dimocarpus longan lour.microrna160 plays a critical role in plant development by negatively regulating the auxin response factors arf10, -16, and -17. however, the ways in which mir160 expression is regulated at the transcriptional level, and how mir160 interacts with its targets during plant embryo development, remain unknown. here, we studied the regulatory relationships among endogenous target mimics (etms), and mir160 and its targets, and their involvement in hormone signaling and somatic embryogenesis (se) in dim ...201526594219
longan (dimocarpus longan lour.) inhibits lipopolysaccharide-stimulated nitric oxide production in macrophages by suppressing nf-κb and ap-1 signaling pathways.flower, seed, and fruit of longan (dimocarpus longan lour.) have been used in the traditional chinese medicine (tcm) serving as a common herb in relief of swelling which can be applied in cases of inflammatory diseases. however, the scientific evidence related to their effects on inflammation especially the possible cellular and molecular mechanisms of longan need to be clarified.201626721218
microrna390-directed tas3 cleavage leads to the production of tasirna-arf3/4 during somatic embryogenesis in dimocarpus longan lour.trans-acting short-interfering rnas (tasirnas) originate from tas3 families through microrna (mirna) 390-guided cleavage of primary transcripts and target auxin response factors (arf3/-4), which are involved in the normal development of lateral roots and flowers in plants. however, their roles in embryo development are still unclear. here, the pathway mir390-tas3-arf3/-4 was identified systematically for the first time during somatic embryo development in dimocarpus longan. we identified the mir ...201526734029
application of longan shell as non-conventional low-cost adsorbent for the removal of cationic dye from aqueous solution.with simple physical treatment, adsorption potential of longan shell for the methylene blue (mb) from aqueous solution was studied as a low-cost material under the conditions of adsorbent dosage (1-6 g/l), initial solution ph (2-12), contact time (5-180 min), temperature (293, 313, 313 k) and initial dye concentration (100-500 mg/l). the sem images and ftir spectra of longan shell before and after dye adsorption were analyzed to understand the adsorption process of mb onto longan shell. the kine ...201626878356
longan (dimocarpus longan lour.) fruit extract stimulates osteoblast differentiation via erk1/2-dependent runx2 activation.longan (dimocarpus longan lour.) has been used as a traditional oriental medicine and possesses a number of physiological activities. in this study, we used cell-based herbal extract screening to identify longan fruit extract (lfe) as an activator of osteoblast differentiation. lfe up-regulated alkaline phosphatase (alp) activity, induced mineralization, and activated runx2 gene expression in mc3t3-e1 cells. furthermore, treatment of mc3t3-e1 cells with lfe promoted the phosphorylation of extrac ...201626975768
molecular cloning, structural and expression profiling of dlran genes during somatic embryogenesis in dimocarpus longan lour.to clone and examine expression profiles of dlran genes during somatic embryogenesis in dimocarpus longan lour. thirty cdna sequences and two genomic sequences encoding dlran proteins were isolated from longan embryogenic cultures. structural analysis of dlran genes revealed that the longan ran gene family is more expanded than that of arabidopsis. expression analysis of dlran genes during somatic embryogenesis uncovered a high abundance of dlran genes in early embryogenic cultures and heart- an ...201627026877
hydrogen peroxide induced changes in energy status and respiration metabolism of harvested longan fruit in relation to pericarp browning.energy status and respiration metabolism of "fuyan" longan fruit treated by hydrogen peroxide (h2o2) and their relationship to pericarp browning were studied. the results displayed that h2o2 significantly increased the respiration rate, increased activities of respiratory terminal oxidases like cytochrome c oxidase (cco) and ascorbic acid oxidase (aao), decreased nad kinase activity, maintained lower contents of nadp and nadph as well as higher amounts of nad and nadh, and accelerated the decrea ...201627213701
ras-related nuclear protein ran3b gene is involved in hormone responses in the embryogenic callus of dimocarpus longan lour.ras-related guanosine triphosphate (gtp)-binding nuclear protein (ran) gtpases function as molecular switches and regulate diverse cellular events in eukaryotes. our previous work suggested that dlran3b is active during longan (dimocarpus longan lour.) somatic embryogenesis (se) processes. herein, subcellular localization of dlran3b was found to be localized in the nucleus and expression profiling of dlran3b was performed during longan se and after exposure to plant hormones (indoleacetic acid ( ...201627271605
evaluation of xanthine oxidase inhibitory potential and in vivo hypouricemic activity of dimocarpus longan lour. extracts.longan is a fruit tree known to contain many phenolic components, which are capable of protecting people from oxidative damage through an anti-inflammatory mechanism. it may be also worthwhile to study the effect on lowering uric acid activity.201627279708
biosynthesis, antibacterial activity and anticancer effects against prostate cancer (pc-3) cells of silver nanoparticles using dimocarpus longan lour. peel extract.metal nanoparticles, particularly silver nanoparticles (agnps), are developing more important roles as diagnostic and therapeutic agents for cancers with the improvement of eco-friendly synthesis methods. this study demonstrates the biosynthesis, antibacterial activity, and anticancer effects of silver nanoparticles using dimocarpus longan lour. peel aqueous extract. the agnps were characterized by uv-vis absorption spectroscopy, x-ray diffraction (xrd), high-resolution transmission electron mic ...201627316741
quantification of toxins in soapberry (sapindaceae) arils: hypoglycin a and methylenecyclopropylglycine.methylenecyclopropylglycine (mcpg) and hypoglycin a (hga) are naturally occurring amino acids found in some soapberry fruits. fatalities have been reported worldwide as a result of hga ingestion, and exposure to mcpg has been implicated recently in the asian outbreaks of hypoglycemic encephalopathy. in response to an outbreak linked to soapberry ingestion, the authors developed the first method to simultaneously quantify mcpg and hga in soapberry fruits from 1 to 10 000 ppm of both toxins in dri ...201627367968
validation of reference genes for rt-qpcr studies of gene expression in preharvest and postharvest longan fruits under different experimental conditions.reverse transcription quantitative pcr (rt-qpcr) as the accurate and sensitive method is use for gene expression analysis, but the veracity and reliability result depends on whether select appropriate reference gene or not. to date, several reliable reference gene validations have been reported in fruits trees, but none have been done on preharvest and postharvest longan fruits. in this study, 12 candidate reference genes, namely, cyp, rpl, gapdh, tua, tub, fe-sod, mn-sod, cu/zn-sod, 18srrna, ac ...201627375640
photocatalytic and antibacterial response of biosynthesized gold nanoparticles.increase in the bacterial resistance to available antibiotics and water contamination by different toxic organic dyes are both severe problems throughout the world. to overcome these concerns, new methodologies including synthesis of nontoxic, human friendly and efficient nanoparticles is required. these nanoparticles not even inhibit the growth of microorganisms but are also effective in the degradation of toxic organics in waste water thus providing a clean and human friendly environment. the ...201627394010
[assessment on the yield loss risk of longan caused by cold damage in south china].using daily climate variables gathered from 64 meteorological stations in south china from 1961 to 2012, recognized hazard indicators about disaster grades of cold damage for longan, and methods on agricultural meteorological disasters risk and simulation technology, the yield loss risks of longan caused by cold damage in south china during different developmental periods were assessed. the results showed that during the period of physiologic differentiation of flower bud, the disasters of longa ...201627396131
impact of precooling and controlled-atmosphere storage on γ-aminobutyric acid (gaba) accumulation in longan (dimocarpus longan lour.) fruit.longan (dimocarpus longan lour.) fruit cultivars 'chuliang' and 'shixia' were analyzed for γ-aminobutyric acid (gaba) accumulation after precooling and in controlled-atmosphere storage. fruit were exposed to 5% o2 plus 3%, 5%, or 10% co2 at 4 °c, and gaba and associated enzymes, aril firmness, and pericarp color were measured. aril softening and pericarp browning were delayed by 5% co2 + 5% o2. gaba concentrations and glutamate decarboxylase (gad; ec 4.1.1.15) activities declined during storage ...201627412947
identification of differentially expressed genes implicated in peel color (red and green) of dimocarpus confinis.nowadays, there are few reports about regulatory genes implicated in peel color of longan. the basic genetic research of longan has been in stagnation for a long time as a lack of transcriptomic and genetic information. to predict candidate genes associated with peel color, gene functional annotation and coding sequence prediction were used to perform functional annotation for our assembled unigenes and investigate differentially expressed genes (degs) of fruitlet peels from longli (dimocarpus c ...201627468388
inhibitory effects of propyl gallate on membrane lipids metabolism and its relation to increasing storability of harvested longan fruit.effects of propyl gallate on membrane lipids metabolism and its relation to storability of harvested longan fruits were studied. the results showed that the propyl gallate-treated longans maintained lower activities of pericarp phospholipase d (pld), lipase and lipoxygenase (lox) than those in control fruits. such treatments could maintain higher levels of pericarp unsaturated fatty acids (usfas), higher pericarp indices of unsaturated fatty acids (iufa), and higher pericarp ratio of unsaturated ...201727664618
antioxidant and catalytic applications of silver nanoparticles using dimocarpus longan seed extract as a reducing and stabilizing agent.in this study, a simple and environmental friendly method was developed for the synthesis of silver nanoparticles (ag-nps) using dimocarpus longan seed extract as a source of reducing and stabilizing agent. the appearance of a surface plasmon resonance peak at 432nm confirmed the synthesis of silver nanoparticles (uv-visible spectroscopy). the biosynthesized ag-nps were face centered cubic structures (xrd) with an approximate particle size of 40nm (tem). optimization study revealed that 10ml of ...201627723492
anti-inflammatory effect of longan seed extract in carrageenan stimulated sprague-dawley rats.longan seeds have been used as a folk medicine in china. longan seed extract (lse) is known for antioxidative, antiproliferative, hypoglycemic, and hypouremic effects. however, its anti-inflammatory effect has not been shown.201627746869
bioguided fraction and isolation of the antitumor components from phyllanthus niruri l.phyllanthus niruri l., a well-known medicinal plant, has been used as a folk antitumor remedy in the worldwide scale. however, the antitumor components in p. niruri have not been reported. in order to verify the antitumor components of p. niruri and the plants which have the high content of these components, we isolated the antitumor components with bioguided fraction and isolation, by different chromatographic methods from the ethyl acetate fraction of p. niruri., and identified them as ethyl b ...201627777954
ultrahigh pressure-assisted enzymatic extraction maximizes the yield of longan pulp polysaccharides and their acetylcholinesterase inhibitory activity in vitro.an extraction method employing ultrahigh pressure-assisted enzymatic treatment was developed and optimized by response surface methodology to increase the yield of longan pulp polysaccharides (lp-ue). a maximum polysaccharides yield of 8.55% was obtained under the optimal conditions of 407mpa ultrahigh pressure maintained for 6min with an enzyme to pretreated material ratio of 1:100, an enzymolysis time of 1.7h and a water to pretreated material ratio of 42ml/g. subsequently, the physicochemical ...201727908719
long-term effectiveness of plasma-derived hepatitis b vaccine 22-28 years after immunization in a hepatitis b virus endemic rural area: is an adult booster dose needed?longan county is considered a highly endemic area for hepatitis b virus (hbv). the plasma-derived vaccine has been used in newborns in this area since 1987. a cross-sectional survey was conducted to evaluate the long-term effectiveness of this vaccine. in total, 1634 participants born during 1987-1993 and who had received a series of plasma-derived hb vaccinations at ages 0, 1, and 6 months were enrolled. serological hbv markers were detected and compared with previous survey data. overall the p ...201728065199
molecular tracing of the origin of six different plant species in bee honey using real-time pcr.the quality of honey is significantly influencedby floral origin. mislabeling floral species occursfrequently in beehoney products. to protectconsumers from economic fraud and maintain afair market environment, methods to identify floralspecies in honey are necessary. in our study, realtimepcrs were established, targeting six honeytypes mainly produced in china (canola, chinesemilkvetch, chinese chaste tree, locust tree, litchi,and longan). sensitivity testing on dna from planttissues exhibited ...201728094000
volatile organic compounds of thai honeys produced from several floral sources by different honey bee species.the volatile organic compounds (vocs) of four monofloral and one multifloral of thai honeys produced by apis cerana, apis dorsata and apis mellifera were analyzed by headspace solid-phase microextraction (hs-spme) followed by gas chromatography and mass spectrometry (gc-ms). the floral sources were longan, sunflower, coffee, wild flowers (wild) and lychee. honey originating from longan had more vocs than all other floral sources. sunflower honey had the least numbers of vocs. cis-linalool oxide, ...201728192487
hydrogen peroxide-induced pericarp browning of harvested longan fruit in association with energy metabolism.energy metabolism of "fuyan" longan fruit treated with hydrogen peroxide (h2o2), the most stable of the reactive oxygen, and its relationship to pericarp browning were investigated in this work. the results displayed that h2o2 significantly decreased contents of adenosine triphosphate (atp) and adenosine diphosphate (adp). it also inhibited activities of h(+)-atpase, ca(2+)-atpase and mg(2+)-atpase in membranes of plasma, vacuole and mitochondria during the early-storage and mid-storage (except ...201728193430
differential gene expression of longan under simulated acid rain stress.differential gene expression profile was studied in dimocarpus longan lour. in response to treatments of simulated acid rain with ph 2.5, 3.5, and a control (ph 5.6) using differential display reverse transcription polymerase chain reaction (ddrt-pcr). results showed that mrna differential display conditions were optimized to find an expressed sequence tag (est) related with acid rain stress. the potential encoding products had 80% similarity with a transcription initiation factor iif of gossypi ...201728299408
dnp and atp induced alteration in disease development of phomopsis longanae chi-inoculated longan fruit by acting on energy status and reactive oxygen species production-scavenging system.as compared with p. longanae-inoculated longans, dnp treatment for p. longanae-inoculated longans exhibited higher fruit disease index and pericarp browning index, lower atp amount and energy charge level, lower activities of sod, cat and apx, lower amounts of asa and gsh, lower levels of dpph radical scavenging activity and reducing power, higher o2(-) generating rate and mda amount. however, supply of atp for p. longanae-inoculated longans showed the contrary effects. these results gave convin ...201728317755
longan seed and peel as environmentally friendly corrosion inhibitor for mild steel in acid solution: experimental and theoretical studies.longan seed and peel were extracted and used for impeding the corrosion of mild steel in hydrochloric acid solution. experimental analyses indicate that the water extract of longan's seed and peel, a mixed type inhibitor, can be chemically adsorbed onto the steel surface, and its inhibition effect rises with increasing extract concentration. to understand the adsorption mechanism and the contributions of the four main compounds of the extract to corrosion protection effect, quantum chemical meth ...201728365436
genome-wide sequencing of longan (dimocarpus longan lour.) provides insights into molecular basis of its polyphenol-rich characteristics.longan ( dimocarpus longan lour.), an important subtropical fruit in the family sapindaceae , is grown in more than ten countries. longan is an edible drupe fruit and a source of traditional medicine with polyphenol-rich traits. tree size, alternate bearing, and witches' broom disease still pose serious problems. to gain insights into the genomic basis of longan traits, a draft genome sequence was assembled.201728368449
neuroprotective activity of cerebrosides from typhonium giganteum by regulating caspase-3 and bax/bcl-2 signaling pathways in pc12 cells.an investigation of the potential neuroprotective natural product constituents of the rhizomes of typhonium giganteum led to the isolation of two new cerebrosides, typhonosides b (1) and c (2), along with 11 known analogues (3-13). the structures of compounds 1 and 2 were elucidated by spectroscopic data interpretation. the activity of these compounds against glutamate-induced cell apoptosis was investigated in pc12 cells. all compounds exhibited such activity, which was related to the length of ...201728394604
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