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[pharmacologic effects of an extract of arillus longan (lour.) steud. and gecko].alg is an extract from arillus longan and gecko. our experiment has shown that alg ig 20 ml/kg/d x 10 elevates significantly tolerance in mice under low and high temperatures and anoxia, and also increases the body-weight of normal mice, while alg ig 15 ml/kg/d x 14 protects body-weight from decreasing in reserpinized mice. an increase in spleen-weight has also been observed in normal mice with alg ig 20 ml/kg/d x 7, and alg ig 15 ml/kg/d x 10 elevates the clearance rate of iv charcoal particles ...19892511863
antimutagenic unusual amino acids from plants.five unusual amino acids were identified as antimutagens against spontaneous mutation of salmonella typhimurium ta100: l-azetidine-2-carboxylic acid (1) from liliaceae plants, alpha-(methylenecyclopropyl)glycine (2) from litchi chinensis seeds, and 2-amino-4-methylhex-5-ynoic acid (3), hypoglycin a (4), and (2s,4r)-2-amino-4-hydroxyhept-6-ynoic acid (5) from euphoria longana seeds. the absolute stereochemistry of 5 was determined by its chiral synthesis from l-allylglycine, proving that 5 is the ...19853935481
adenosine, the anxiolytic-like principle of the arillus of euphoria longana.according to traditional usage, the extract of longan arillus was tested for its anxiolytic-like effect by application of the vogel-type anti-conflict method in mice. the extract indicated significant activity at a dose of 2 g/kg, s.c., and results of the bioassay-oriented isolation revealed adenosine to be the active principle. adenosine produced the anti-conflict effect significantly at a dose of 30 mg/kg, s.c. adenine, uridine, and 5-methyluridine did not exhibit the effect, although these co ...199910193200
hot water immersion to ensure quarantine security for cryptophlebia spp. (lepidoptera: tortricidae) in lychee and longan exported from hawaii.we determined whether immersion in 49 degrees c water for 20 min, a quarantine treatment developed for disinfestation of fruit flies in lychee, litchi chinensis sonn., and longan, dimocarpus longan (lourd.) steud., exported from hawaii, would also disinfest fruit of two species of cryptophlebia. the pattern of tolerance to heat in cryptophlebia illepida (butler) was generally eggs < neonates < early instars = late instars < pupae. no c. illepida survived immersion for 16 or 20 min. late fourth a ...200111681696
long-term effectiveness of infancy low-dose hepatitis b vaccine immunization in zhuang minority area in china.aim:to observe the long-term effectiveness of low-dose immunization strategy and risk factors of hbsag carriers in immunized children of zhuang minorities of longan county in the 9th year after infancy immunization.methods:two epidemiologic methods,a cross-sectional follow-up study and a case-control study,were used for the evaluation of the serological effect and the determination of the risk factors. hepatitis b virus markers were detected with radioimmunoassay.results:the protective anti-hbs- ...199911819408
differential effects of foods traditionally regarded as 'heating' and 'cooling' on prostaglandin e(2) production by a macrophage cell line.some components of natural foods may enhance or inhibit prostaglandin formation and potentially affect the inflammation condition. a macrophage cell line, raw264.7, was employed to examine the effects of foods traditionally regarded as 'heating' or 'cooling' on the production of pge(2), a well-known proinflammatory mediator. foods traditionally regarded as 'heating' (litchi, longan, and dried longan) or 'cooling' (chrysanthemum flower, bitter gourd, and lotus seed plumule) were extracted sequent ...201612432225
cerebrosides from longan arillus.from the pulp of euphoria longana (longan arillus), three cerebroside molecular species have been isolated. six known cerebrosides, soyacerebrosides i and ii, 1-o-beta-d-glucopyranosyl-(2s,3r,4e,8e)-2-(2'-lignoceroylamino)-4,8-octadecadiene-1,3-diol (longan cerebroside i) and its 8z isomer (longan cerebroside ii), momor-cerebroside i, and phytolacca cerebroside, were identified as major components of these cerebroside molecular species. all the cerebrosides were shown to be a mixture of geometri ...200312643590
[prevalence of hepatitis b virus core promoter mutant isolated from asymptomatic carriers from areas with higher and lower incidence of hepatocellular carcinoma in guangxi].to understand the prevalence of hbv core promoter mutant (t1762 a1764 mutant) isolated from asymptomatic carriers from areas with higher and lower incidence of hepatocellular carcinoma (hcc) in guangxi.200312869995
assessment of hydroxyl radical generation and radical scavenging activity of chinese medicinal herbs using gc-ms.aqueous extracts of ten chinese herbs were evaluated for their radical scavenging activity by a gc-ms method based on the fenton reaction system. hydroxylation of salicylate and phenylalanine is widely used as an index of hydroxyl radical formation in vivo and in vitro. a problem associated with quantifying product from such reactions is the generation of complex reaction products that increase background 'noise' and reduce sensitivity for the target product. the aim of this investigation was to ...200314599346
development, characterization and variability analysis of microsatellites in lychee (litchi chinensis sonn., sapindaceae).we report 12 microsatellites enriched in ct repeats obtained from a genomic library of the lychee ( litchi chinensis sonn.) cultivar mauritius. the polymorphisms revealed by these microsatellites were evaluated in a collection of 21 lychee cultivars. a total of 59 fragments were detected with these 12 ssrs, with an average of 4.9 bands/ssr. three primer pairs seem to amplify more than a single locus. the mean expected and observed heterozygosities over the 9 single-locus ssrs averaged 0.571 (ran ...200414605809
[genetic diversity of common wild rice (oryza rufipogon griff) by using ssr markers and phenotypic traits in guangxi province].in this paper, allelic variation and genetic diversity among geographies and growth habit types were studied by using 223 accessions of common wild rice primary core collection in guangxi province, with 34 ssr primers locating on 12 chromosomes of rice and 19 phenotypic traits. in the results, 24.91 alleles were detected per locus on average with a range from 7 to 48. compared to the cultivated rice, the wild rice showed more allelic variations. the ratio of heterozygote of ssr locus was 32.01% ...200415493143
[full-length sequence of hepatitis b virus isolated from high incidence hepatocellular carcinoma area-longan county].to understand full-length sequence of hbv isolated from high incidence hepatocellular carcinoma area-longan county, guangxi.200415640866
identification and quantification of polyphenolic compounds in longan (euphoria longana lam.) fruit.regular consumption of fruit and vegetables is associated with a lower risk of some chronic diseases including various forms of cancer and cardiovascular diseases. the health-promoting potential of these foods may be due, in part, to the phytochemical bioactive compounds present in the plants. fruit of euphoria longana lam. (longan) are consumed throughout asia and are a major crop in thailand. in the present study phytochemicals were extracted with 70% methanol from peel, pulp, and seed tissues ...200515740011
phytate and fiber content in thai fruits commonly consumed by diabetic patients.the amount of phytate and total dietary fiber of 6 popular tropical fruits eaten by diabetic patient residing in bangkok were studied. they were dragon fruit, durian, guava, longan, mango, and pineapple. fruit containing the highest content of total dietary fiber was guava which had about 2.7%. longan contained the lowest fiber content equal to 0.19%. the highest content of phytate was found in pineapple about 0.09% and the lowest content was in longan about 0.037%. it was noticed that longan co ...200415822538
[the relationship between the desiccation-induced browning and the metabolism of active oxygen and phenolics in pericarp of postharvest longan fruit].longan (dimocarpus longan lour.) fruits are very susceptible to water loss and pericarp browning, and postharvest pericarp browning is the most important factors degrading the quality of longan fruit and shorting storage life. pericarp browning has been attributed to desiccation, chilling, heat stress, senescence and pest or pathogen attack. desiccation is the most main factor of induced-pericarp browning in longan. the relationship between water loss from pericarp and pericarp browning in longa ...200515961904
isolation and structure elucidation of phenolic compounds from longan (dimocarpus longan lour.) seed by high-performance liquid chromatography-electrospray ionization mass spectrometry.high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (hplc-esi-ms) analysis revealed the phenolics profile of longan (dimocarpus longan lour.) seed. gallic acid, ellagic acid, monogalloyl-glucose, monogalloyl-diglucose, digalloyl-diglucose, penta- to heptagalloyl-glucose, ellagic acid-pentose conjugate, galloyl-hhdp (hexahydroxydiphenoyl)-glucopyranose, pentagalloy-hhdp-glucopyranose, procyanidin a-type dimer, procyanidin b2 and quercetin-3-o-rhamnoside w ...200516106708
[roles of olfaction and vision in orientation behavior of adult campylomma chinensis schuh (hemiptera: miridae) toward lantana plants (verbanaceae)].investigations were carried out on the orientation behavior of adult campylomma chinensis schuh (hemiptera: miridae) toward plant hosts lantana camara, l. caniara cv. 'flava', and l. montevidensis (spreng. ) briq. (verbanaceae). surveys on three lantana plants showed that the distribution of c. chinensis in inflorescences was not related to floral color (partial correlation coefficient was 0.240, p = 0. 147, n = 40), but to the number of thrips hawiiensis morgan (partial correlation coefficient ...200516252876
[population dynamics of bactrocera dorsalis (diptera: tephritidae) in xishuangbanna of southern yunnan].in the study, an all year round monitoring through lure-traps in 1997, 2000 and 2003 was conducted on the population dynamics of oriental fruit fly, bactrocera dorsalis (diptera: tephritidae) in xishuangbanna of southern yunnan, and the factors in relation with its population fluctuation, including temperature, rainfall, and host species were analyzed. the fruit fly occurred year-round in xishuangbanna, with a low population level from november to february, and increased steadily from march to j ...200516252878
cadmium contamination in orchard soils and fruit trees and its potential health risk in guangzhou, china.this study examines cadmium (cd) contamination in orchard soils and fruit trees in guangzhou, china, and assesses its potential health risk. soils and tissues samples of three species of fruit trees were collected from three orchards. the average soil cd concentration was 1.27, 1.84 and 0.68 mg/kg in orchards i, ii, and iii, respectively. the carambola (averrhoa carambola) accumulated exceptionally high concentrations of cd (7.57, 10.84, 9.01 and 2.15 mg/kg dw in root, twig, leaf and fruit, resp ...200616377042
mode of grasp, materials, and grooved chopstick tip on gripping performance and evaluation.this study evaluated the effects of the mode of grasping, chopstick materials, and the groove of the chopstick-tip on gripping performance of 32 participants, among whom 16 were accustomed to using chopsticks with a pliers-grasping mode and the other 16 with a scissors-grasping mode. based on a random sequence, 8 kinds of chopsticks with different combinations (materials: bamboo, wood, plastic, and stainless steel, with and without grooves in the chopstick-tip) were used to carry out the action ...200616671604
total antioxidant activity and fiber content of select florida-grown tropical fruits.fourteen tropical fruits from south florida (red guava, white guava, carambola, red pitaya (red dragon), white pitaya (white dragon), mamey sapote, sapodilla, lychee, longan, green mango, ripe mango, green papaya, and ripe papaya) were evaluated for antioxidant activity, total soluble phenolics (tsp), total ascorbic acid (taa), total dietary fiber (tdf), and pectin. orac (oxygen radical absorbance capacity) and dpph (1,1-diphenyl-2-picrylhydrazyl, radical scavenging activity) assays were used to ...200616968105
evaluation of free radical scavenging and antityrosinase activities of standardized longan fruit extract.the protective effects of fruits and vegetables against chronic diseases have been attributed to the antioxidant properties of some secondary metabolites present in these foods. plant polyphenols have been reported to exhibit bioactive properties, and in particular antioxidant activities. longan seeds are found to contain high levels of some beneficial polyphenolic compounds such as corilagin, gallic acid and ellagic acid. the present study examined the free radical scavenging activity of longan ...200717049706
[the extraction and identification of total flavanone from longan pericarp].in order to make use of the resources of total flavanone in pericarp of longan.200617076235
[changes in cell wall components and cell wall-degrading enzyme activities of postharvest longan fruit during aril breakdown].longan (dimocarpus longan lour.) fruits are very susceptible to pericarp browning and aril breakdown, and postharvest aril breakdown is one of the most important factors degrading the quality and shorting storage life of longan fruit. changes in aril breakdown index, cell wall components and cell wall-degrading enzyme activities in aril of longan cv. fuyan fruits using sealed polyethylene film bags (0.015 mm thick) at (10+/-1) degrees c were investigated. the main results were as follows. develo ...200717452799
a case of food allergy due to longan fruit. 200717458440
identification of two polyphenolic compounds with antioxidant activities in longan pericarp tissues.longan fruits contain a significant amount of polyphenols. in the present study, polyphenols were extracted from longan pericarp tissues, and then two representative polyphenols were separated and purified by polyamide column chromatography, sephadex lh-20 column chromatography, and silica gel column chromatography. on the basis of 1h nmr, 13c nmr, and electrospray ionization mass spectrometric (esi-ms) data, the two compounds were identified as 4-o-methylgallic acid and (-)-epicatechin, respect ...200717579438
hot-water immersion quarantine treatment against mediterranean fruit fly and oriental fruit fly (diptera: tephritidae) eggs and larvae in litchi and longan fruit exported from hawaii.immersion of litchi fruit in 49 degrees c water for 20 min followed by hydrocooling in ambient (24 +/- 4 degrees c) temperature water for 20 min was tested as a quarantine treatment against potential infestations of mediterranean fruit fly, ceratitis capitata (wiedemann); and oriental fruit fly, bactrocera dorsalis hendel, eggs or larvae in hawaiian litchi, litchi chinensis sonnerat. the 49 degrees c hot-water immersion of litchi provided probit 9 (99.9968% mortality with >95% confidence) quaran ...200717849856
suppressive effect of a proanthocyanidin-rich extract from longan (dimocarpus longan lour.) flowers on nitric oxide production in lps-stimulated macrophage cells.anthocyanidins found in certain flowers have been shown to act as strong antioxidants in various systems, exhibiting multiple biological actions. the antioxidative effects of water extract and ethanolic extract of longan (dimocarpus longan lour.) flowers were evaluated by radical scavenging activity and compared to those of gallic acid, myricetin, and epigallocatechin gallate. in this study, the suppressive effects of longan flower extracts on nitric oxide and prostaglandin e2 production were in ...200718052097
toxic effects of chlorate on three plant species inoculated with arbuscular mycorrhizal fungi.pot experiments were conducted to examine the toxic effects of chlorate on bermudagrass, bahiagrass, and longan seedling with a focus on arbuscular mycorrhizal fungi-plant associations. the results show that application of chlorate could cause slight soil acidification, but the resulting ph was still around 5.5, which is unlikely to adversely affect plant growth. increase in the application rate of chlorate resulted in a decrease in colonization rate of arbuscular mycorrhizal fungi in plant root ...200818602693
effects of activated sludge on the degradation of chlorate in soils under varying environmental conditions.incubation experiments were conducted to examine the effects of activated sludge on degradation of chlorate in soils. the results show that application of activated sludge could significantly promote the decomposition of soil chlorate though the degradation rate of chlorate did not necessarily increase with increasing application rate of the sludge. the effectiveness of activated sludge on soil chlorate degradation was significantly affected by temperature, moisture content and ph. there is a te ...200918621481
antioxidative activity and active components of longan (dimocarpus longan lour.) flower extracts.three different solvent extracts (methanol, ethyl acetate, and n-hexane) of longan ( dimocarpus longan lour.) flowers were assayed with three different antioxidant capacity methods, namely, the dpph free radical scavenging effect, the oxygen radical absorbance capacity (orac) assay, and the inhibition of cu(2+)-induced oxidation of human low-density lipoprotein (ldl). it was revealed that the methanol extract has the best antioxidative activity, followed by ethyl acetate and n-hexane extracts. t ...200818662011
antioxidant activity, ascorbic acid and total phenol of exotic fruits occurring in brazil.the antioxidant activity, ascorbic acid and phenolic content were studied in 10 exotic fruits from brazil: abiu, acerola, wax jambu, cashew, mamey sapote, carambola or star fruit, surinam cherry, longan, sapodilla and jaboticaba. the ascorbic acid was determined by 2,6-dichloroindophenol titrimetic methods and total phenols were measured colorimetrically using the folin-ciocalteu reagent. the antioxidant activity was investigated with three different methods: hypochlorous acid scavenging activit ...200918785051
a novel stopped flow injection-amperometric procedure for the determination of chlorate.a novel stopped flow injection-amperometric (sfi-amp) procedure for determination of chlorate has been developed. the reaction of chlorate with excess potassium iodide and hydrochloric acid, forming iodine/triiodide that is further electrochemically reduced at a glassy carbon electrode at +200mv versus ag/agcl electrode is employed. in order to increase sensitivity without using of too high acid concentration, promoting of the reaction by increasing reaction time and temperature can be carried o ...200518970344
effect of a proanthocyanidin-rich extract from longan flower on markers of metabolic syndrome in fructose-fed rats.recent evidence strongly suggests that oxidative stress due to redox imbalance is highly associated with metabolic syndrome. the objective of this study was to evaluate the effect of the supplementation of longan flower water extract (lfwe), which showed powerful antioxidative activity in vitro, on markers of metabolic syndrome in a fructose-fed rat model. male sprague-dawley rats were randomly divided into four groups: group c, fed with standard purina chow; group f, fed with high-fructose diet ...200818973337
enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultra-high-pressure-assisted extraction.the health benefits of fruits acting against chronic diseases are ascribed to their antioxidant activities which are mainly responsible due to the presence of phenolic compounds. the use of ultra-high-pressure-assisted extraction (uhpe) has shown great advantages for the extraction of these phenolic compounds from longan fruit pericarp (lfp). studies were carried out to investigate the effects of uhpe at pressures of 200, 300, 400 and 500 mpa on total phenolic contents, extraction yield, antioxi ...201019345542
structural characteristics and antioxidant activities of oligosaccharides from longan fruit pericarp.ultrasonic assisted extraction was employed to extract oligosaccharides from longan fruit pericarp (olfp). a box-behnken design was applied to investigate the effects of ultrasonic temperature (30-70 degrees c), power (120-300 w), and time (10-50 min) on olfp recovery. the model showed a good agreement with the experimental results on the basis of r(2) of 0.9655 and p-value <0.05. from response surface plots, ultrasonic power, time, and temperature exhibited independent and interactive effects o ...200919739643
effects of fruit extracts on the formation of acrylamide in model reactions and fried potato crisps.natural products extracted from plants and fruits have attracted increasing attention for the development of effective inhibitors against the formation of acrylamide during food processing. in this study, six fruit extracts (apple, blueberry, mangosteen, longan, dragon fruit with white flesh, and dragon fruit with red flesh) were compared for their activities against acrylamide formation in chemical models containing equal molar quantities of glucose and asparagine in distilled water (160 degree ...201019925016
comparative proteomic analysis of longan (dimocarpus longan lour.) seed abortion.two-dimensional gel electrophoresis (2-de), coupled with mass spectroscopy, was used to study seed abortion in dimocarpus longan lour. (cv. minjiao 64-1) by comparing normal and aborted seeds at three developmental stages. more than 1,000 protein spots were reproducibly detected in 2-de gels, with 43 protein spots being significantly altered in their intensity between normal and aborted seeds at least at one stage. thirty-five proteins were identified by matrix-assisted laser desorption ionizati ...201020049611
the memory-enhancing effects of euphoria longan fruit extract in mice.the fruit of euphoria longan (lour.) steud. (sapindaceae) is sweet and edible. dried euphoria longan fruit is prescribed as a tonic and for the treatment of forgetfulness, insomnia, or palpitations caused by fright in traditional chinese medicine. the effects of aqueous extract of euphoria longan fruit (ele) on learning and memory and their underlying mechanisms were investigated.201020064595
antiobesity and hypolipidemic effects of polyphenol-rich longan (dimocarpus longans lour.) flower water extract in hypercaloric-dietary rats.plenty of polyphenols, i.e. phenolic acids and flavonoids, were found in longan flower water extract (lfwe) through spectrophotometric and hplc analyses. antiobesity and hypolipidemic effects of polyphenol-rich longan flower water extract (lfwe) were investigated in this study. eight male rats per group were assigned randomly to one of the following dietary groups: (1) normal-caloric diet and pure water (ncd + ndw); (2) hypercaloric diet and pure water (hcd + ndw); (3) hcd and 1.25% (w/v) lfwe ( ...201020088600
longan flower extract inhibits the growth of colorectal carcinoma.longan flower extract (lfe) has been shown to exhibit free radical scavenging ability and anti-inflammatory effects. however, the effect of lfe treatment on the growth of colorectal cancer cells has not been evaluated. this study investigated the effect of lfe on two colorectal cancer cell lines, sw-480 and colo 320dm, and the possible mechanisms involved. lfe-treated cells were assessed for viability by trypan blue exclusion, for in vitro tumorigenesis by seeding cells in soft agar to allow anc ...201020099197
preliminary observations on the antifatigue effects of longan (dimocarpus longan lour.) seed polysaccharides.the antifatigue effects of the hot-water extract of longan (dimocarpus longan lour.) seeds were studied in mice. longan seed polysaccharides were administered at doses of 50, 100, 200 and 400 mg/kg and antifatigue activity was evaluated using a swimming test, along with the determination of serum urea nitrogen, hepatic glycogen and blood lactic acid content. the results show that longan seed polysaccharides, in doses ranging from 50 to 100 mg/kg, extended swimming time, increased hepatic glycoge ...201020104503
use of the bcr sequential extraction procedure for the study of metal availability to plants.to investigate the mobility and availability of metals from soil to plant, concentrations of zinc (zn), copper (cu), lead (pb), cadmium (cd), mercury (hg) and arsenic (as) in topsoils and plants (lettuce, scallion, celery, tomato, carambola, wampee and longan) collected from the area around a petrochemical complex in guangzhou, china, were analyzed. the modified european community bureau of reference (bcr) three-step sequential extraction procedure was applied to determine the concentration of m ...201020145888
longan shell as novel biomacromolecular sorbent for highly selective removal of lead and mercury ions.strongly selective adsorbability of natural longan (euphoria longan) shell as a biomacromolecular sorbent toward heavy metal ions from aqueous solutions has been discovered and successfully optimized by facilely controlling the shell dosage, sorption time, ph value, and ion concentration. the sorption well-fits the langmuir isotherm and obeys the pseudo-second-order kinetics. the maximum adsorptivity of pb(ii) and hg(ii) is 99.3 and 99.1%, respectively, with short equilibrium sorption time. the ...201020175512
control of rotting and browning of longan fruit cv. biew kiew after harvested by sulphur dioxide treatment under various storage temperatures.the experiment of longan fruit cv. biew kiew, untreated (control) and treated with so2 treatment were stored under 2 +/- 2 and 7 +/- 2 degrees c for 0, 2, 4, 6 and 8 weeks were studied. the treatment of fresh longan fruit with so2 fumigation combined with the suitable storage condition improved the overall longan fruit quality, especially on inner and outer peel tissue and aril color than no so2 treatment. treatment stabilizes peel color with no subsequent loss of color during storage (fruit col ...200920180317
a comparative identification of ochratoxin a in longan fruit pulp by high performance liquid chromatography-fluorescence detection and electron spray ionization-mass spectrometry.harvested longan (dimocarpus longan lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. ochratoxin a (ota) is a kind of mycotoxin produced by a number of fungi. in this study, ota was extracted from longan fruit pulp by 80% methanol and then loaded on c-18 solid-phase extraction columns. the extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (hplc-fd) and an electron spray ionization-mass spectrometry (esi-ms), ...201020335938
in vitro antibacterial, cytotoxic and antioxidant activities of plant nephelium longan.the petroleum ether, chloroform and ethyl acetate fractions of ethanol extract of leaf and stem from the plant nephelium longan (fam-sapindaceae) was subjected to antioxidant, antibacterial and cytotoxic activity. all the fractions showed potent antioxidant activity, of which the ethyl acetate and chloroform fraction of leaf demonstrated the strongest antioxidant activity with the ic50 value of 44.28 and 44.31 microg ml(-1), respectively. the petroleum ether extracts (500 microg disc(-1)) of lea ...201020415149
the effect of longan seed polyphenols on colorectal carcinoma cells.polyphenol-rich longan seed extract (lsp) is a free radical scavenger and antioxidant. however, the effect of lsp on the growth of human colorectal carcinoma cells (crc) has not yet been evaluated.201020561027
evaluation of radicals scavenging, immunity-modulatory and antitumor activities of longan polysaccharides with ultrasonic extraction on in s180 tumor mice models.longan (dimocarpus longan lour.) is used as a traditional edible and medicinal material in china. in this paper, the effects of polysaccharides from longan pulp with ultrasonic extraction (uelp) on the radicals scavenging, immunity-modulatory and antitumor activities in s180 tumor mice models were investigated. uelp showed excellent scavenging activity on the hydroxyl radicals and a,a-diphenyl-1-picrylhydrazyl (dpph) radicals, and obtained the almost complete scavenging effect. uelp with medium- ...201020685359
comparison on characterization of longan (dimocarpus longan lour.) polyphenoloxidase using endogenous and exogenous substrates.longan polyphenoloxidase (ppo) was extracted and partially purified using ammonium sulfate precipitation and dialysis. the ppo characterizations were compared using endogenous substrate (-)-epicatechin and exogenous substrate catechol. the optimal ph and optimal temperature for the ppo activity were different when reacting with both substrates. the addition of ethylenediaminetetraacetic acid disodium salt into both substrate-enzyme systems exhibited the same lowest inhibition of the ppo activity ...201020718432
the neuroprotective effect of modified yeoldahanso-tang via autophagy enhancement in models of parkinson's disease.modified yeoldahanso-tang (myh) is a korean herbal formula, containing 10 herbs: pueraria lobata (willd.) ohwi, angelica tenuissima nakai, scutellaria baicalensis georgi, platycodon grandiflorum (jacq), angelicae dahurica, cimicifuga heracleifolia kom, raphanus sativa l., polygala tenuifolia (willd.), acorus gramineus soland. and dimocarpus longan lour. the constitutive ratio of the ten herbs is at 6:4:2:1:2:2:2:4:6:6 in dry weight. myh has been used to treat amnesia, hypochondria and dementia i ...201021172413
studies on the use of "slimax", a chinese herbal mixture, in the treatment of human obesity.a phytochemical preparation known as "slimax" was administered orally to human volunteers for a six week period, using a double-blind experimental method. this preparation is an aqueous extract of the chinese medicinal plants hordeum vulgare , polygonatum multiflorum , dimocarpus longan , ligusticum sinense , lilium brownii and zingiber officinale . treatment with slimax resulted in a significant decrease in parameters such as body weight, waist and hip circumference, and body mass index (bmi), ...200021214436
molecular characterization of high plant species using pcr with primers designed from consensus branch point signal sequences.a novel method is introduced for producing molecular markers in plants using single 15- to 18-mer pcr primers designed from the short conserved consensus branch point signal sequences and standard agarose gel electrophoresis. this method was tested on cultivated peanut and verified to give good fingerprinting results in other plant species (mango, banana, and longan). these single primers, designed from relatively conserved branch point signal sequences within gene introns, should be universal a ...201121264505
effects of polysaccharides of the euphoria longan (lour.) steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism.to investigate the effects of polysaccharides of the euphoria longan (lour.) steud on focal cerebral ischemia/reperfusion(i/r) injury in rats.201121280975
[the effect evaluation of a new tuberculosis management model in rural areas of guangxi].to explore the effect of new model for tuberculosis (tb) control and management, and provide a scientific basis and justification for making tb control strategies in rural communities.201121418816
[svm-based qualitative analysis of muscat hamburg wine produced in tianjin region].the purpose was to achieve the identification of muscat hamburg wines produced in tianjin region through scanning and analyzing dry white wine samples of different grape varieties and regions by infrared spectroscopy technology. a support vector machine (svm) based method was introduced to analyze infrared spectra of dry white wines. the pretreatment processes of the ir spectra were also elaborated, including baseline adjustment, noise elimination, standard normalization and eliminating the main ...201121428068
two gh3 genes from longan are differentially regulated during fruit growth and development.in the present work, two full length cdnas of gh3 genes, named dlgh3.1 and dlgh3.2 were cloned from pericarp and aril tissues of the longan fruit, respectively. three conserved motifs, ssgtsagerk, yasse and yrvgd, as a characteristic of the acyladenylate/thioester forming enzyme superfamily were observed in dlgh3.1 and dlgh3.2 proteins. dlgh3.1 mainly expressed in pericarp tissues while dlgh3.2 accumulated in both the pericarp and aril tissues during fruit growth and development. in addition, na ...201121672614
investigation of plant hormone level changes in shoot tips of longan (dimocarpus longan lour.) treated with potassium chlorate by liquid chromatography-electrospray ionization mass spectrometry.the endogenous levels of indole-3-acetic acid (iaa), gibberellins (gas), abscisic acid (aba) and cytokinins (cks) and their changes were investigated in shoot tips of ten longan (dimocarpus longan lour.) trees for off-season flowering until 60 days after potassium chlorate treatment in comparison with those of ten control (untreated) longan trees. these analytes were extracted and interfering matrices removed with a single mixed-mode solid phase extraction under optimum conditions. the recoverie ...201121726716
physicochemical characteristics and immunomodulatory activities of three polysaccharide-protein complexes of longan pulp.three polysaccharide-protein complexes of longan pulp (lp1-3) were isolated in this work. their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (nk) cells and macrophages in vitro were investigated. the carbohydrate portions of lp1-3 were principally composed of glucose, arabinose and mannose. lp3 displayed the maximal moisture absorption, and the thermal stability of lp2 was obviously higher than that of lp1 and lp3. all of them showed the characteris ...201121778950
Histone deacetylase HD2 interacts with ERF1 and is involved in longan fruit senescence.Histone deacetylation plays an important role in epigenetic control of gene expression. HD2 is a plant-specific histone deacetylase that is able to mediate transcriptional repression in many biological processes. To investigate the epigenetic and transcriptional mechanisms of longan fruit senescence, one histone deacetylase 2-like gene, DlHD2, and two ethylene-responsive factor-like genes, DlERF1 and DlERF2, were cloned and characterized from longan fruit. Expression of these genes was examined ...201221926091
floral reversion mechanism in longan (dimocarpus longan lour.) revealed by proteomic and anatomic analyses.two-dimensional gel electrophoresis (2-de) was used to analyze the proteins related to floral reversion in dimocarpus longan lour. proteins were extracted from buds undergoing the normal process of flowering and from those undergoing floral reversion in three developing stages in d. longan. differentially expressed proteins were identified from the gels after 2-de analysis, which were confirmed using matrix-assisted laser desorption/ionization-time of flying-mass spectroscopy and protein databas ...201222100459
Immunomodulatory Activity of Polysaccharide-Protein Complex of Longan (Dimocarpus longan Lour.) Pulp.The immunomodulatory function of longan pulp polysaccharide-protein complex (LP3) was investigated in immunosuppressed mice models. Compared with the model control, peroral administration of 100 mgkg-1d-1 LP3 could significantly increase/enhance antibody production against chicken red blood cell (CRBC), concanavalin A (ConA)-induced splenocyte proliferation, macrophage phagocytosis, NK cell cytotoxicity against YAC-1 lymphoma cell, and interferon-gamma (INF-?) and interleukin-2 (IL-2) secretion ...201122158685
in vitro antifungal activities of longan (dimocarpus longan lour.) seed extract.longan, dimocarpus longan lour., contains polyphenolic compounds which exhibit several pharmacological properties. this study aims to evaluate antifungal activities of longan fruit extract in comparison to its active compounds. the results showed that longan seed exhibited antifungal activity against the opportunistic yeasts (candida species and cryptococcus neoformans). in contrast, longan pulp and whole fruit did not demonstrate any inhibitory effects. ellagic acid showed the most potent antif ...201222245574
isolation and characterization of ellagitannins as the major polyphenolic components of longan (dimocarpus longan lour) seeds.longan (dimocarpus longan lour, syn. euphoria longan lam.) represents an important fruit in northern thailand and has significant economic impact. the fruit is either consumed fresh or as commercially prepared dried and canned products. the canning industry in thailand produces considerable quantities of waste products, in particular longan seeds. because these seeds may be an exploitable source of natural phenolic antioxidants, it was of interest to identify, purify and quantitate the major pot ...201222277734
inhibitory effects of water extract from longan twigs on mutation and nitric oxide production.this study examines the inhibitory effects of water extract from longan twigs (wlts) on mutation and nitric oxide (no) production. the results show that wlt inhibited the mutagenicity of 2-aminoanthracene (2-aa), an indirect mutagen, and 4-nitroquinoline-n-oxide (4-nqo), a direct mutagen toward salmonella typhimurium ta 98 and ta 100. in addition, wlt in the range 0-0.6 mg/ml showed radical scavenging, reducing activities and chelating activity, as well as decreased lipid oxidative damage. meanw ...201222868111
a new natural lactone from dimocarpus longan lour. seeds.a new natural product named longanlactone was isolated from dimocarpus longan lour. seeds. its structure was determined as 3-(2-acetyl-1h--pyrrol-1-yl)-5-(prop-2-yn-1-yl)dihydrofuran-2(3h)-one by spectroscopic methods and hresims.201222869162
safety evaluation of longan seed extract: acute and repeated oral administration.longan seed extract (lse) contains high levels of beneficial polyphenolic compounds. the present study evaluated acute and repeated dose (4 and 13 weeks) toxicological effects of aqueous extract of longan seed to ensure the safety of utilization of this extract. our study revealed that all treated animals survived through the whole experimental periods without adverse effects observed in either sex of animals after acute and repeated dose (4 and 13 weeks) oral administration of lse. likewise, gr ...201222902805
neuroprotective effects of longan ( dimocarpus longan lour.) flower water extract on mpp+-induced neurotoxicity in rat brain.in this study, the neuroprotective effect of dimocarpus longan lour. flower water extract (lfwe) was investigated. first, an in vitro study showed that lfwe concentration-dependently inhibited lipid peroxidation of brain homogenates incubated at 37 °c. the antioxidative activity of lfwe was more potent than that of glutathione or trolox. furthermore, an ex vivo study found that the basal lipid peroxidation (0 °c) and lipid peroxidation incubated at 37 °c were lower in the brain homogenates of lf ...201222920583
longan seed extract reduces hyperuricemia via modulating urate transporters and suppressing xanthine oxidase activity.hyperuricemia causes gouty arthritis, kidney disease, heart disease, and other diseases. xanthine oxidase (xod) and urate transporters play important roles in urate homeostasis. numerous plants have been identified as xod inhibitors. longan seeds are known to contain high levels of polyphenols such as corilagin, gallic acid and ellagic acid. we examined the effect of longan seed extract on xod inhibition and urate transporters glut1 and glut9 using both in vitro and in vivo assays. the results s ...201222928829
antioxidant and anti-inflammatory properties of longan (dimocarpus longan lour.) pericarp.this study examined the antioxidant and anti-inflammatory activities of the water extract of longan pericarp (wlp). the results showed that wlp exhibited radical scavenging, reducing activity and liposome protection activity. in addition, wlp also inhibited lipopolysaccharide (lps)-induced nitric oxide (no) production in macrophages. further, administration of wlp, in the range of 100-400 mg/kg, showed a concentration-dependent inhibition on paw edema development following carrageenan (carr) tre ...201222966244
structural characteristics and antioxidant activities of polysaccharides from longan seed.ultrasound-assisted extraction was employed to extract polysaccharides from longan seed (lsp), with aids of a box-behnken statistical design to investigate the effects of ultrasonic power, time and liquid/solid ratio on the extraction recovery of the lsp. the structural analysis indicated that arabinose, galactose, glucose and mannose were major components of lsp, with →6)-gal-(→1, glc-(→1 and →6)-glc-(1→ glycosidic linkages. in an in vitro antioxidant activity of the 1,1-diphenyl-2-picryldydraz ...201323218364
activities of free and encapsulated lactobacillus acidophilus la5 or lactobacillus casei 01 in processed longan juices on exposure to simulated gastrointestinal tract.fruit drinks containing probiotics are gaining interest in the global marketplace. for example, longan juice, containing carbohydrate and various bioactive components, is a potentially health-promoting beverage as well as probiotic carrier for human consumption. in this study, high-pressure and thermal processes were applied to eliminate competitive micro-organisms in longan juice prior to the addition of lactobacillus acidophilus la5 or lactobacillus casei 01. the activities of these probiotics ...201323401169
inhibitory effects of propyl gallate on tyrosinase and its application in controlling pericarp browning of harvested longan fruits.tyrosinase (ec 1.14.18.1), also known as polyphenol oxidase (ppo), is a key enzyme in pigment biosynthesis of organisms. the inhibitory effects of propyl gallate on the activity of mushroom tyrosinase and effects of propyl gallate on pericarp browning of harvested longan fruits in relation to phenolic metabolism were investigated. the results showed that propyl gallate could potently inhibit diphenolase activity of tyrosinase. the inhibitor concentration leading to 50% activity lost (ic50) was d ...201323427826
comparison of dynamic viscoelastic and physicochemical properties of pressurised and pasteurised longan juices with xanthan addition.physical and biochemical properties of pressurised and pasteurised longan juices with various xanthan additions, such as viscoelastic behaviour, colour l (lightness), -a(*) (greenness), b(*) (yellowness), δe (total different colours) and bi (browning index) parameters, polyphenol oxidase (ppo) activity, ascorbic acid, gallic acid, ellagic acid, total phenols and antioxidant capacity (dpph assay) were studied. viscoelastic determination indicated that longan juice with 0.15% xanthan addition was ...201223442674
evaluation of suitable reference genes for normalization of microrna expression by real-time reverse transcription pcr analysis during longan somatic embryogenesis.accurate profiling of micrornas (mirnas) is an essential step for understanding both developmental and physiological functions of mirnas. real-time quantitative pcr (qpcr) is being widely used in mirna expression studies, but choosing a suitable reference gene is a crucial factor for correct analysis of results. to date, there has been no systematic evaluation of qpcr reference genes for the study of mirnas during somatic embryogenesis (se) in the longan tree (dimocarpus longan). here, the most ...201323454294
inhibitory effects of dried longan (euphoria longana lam.) seed extract on invasion and matrix metalloproteinases of colon cancer cells.the critical step in colorectal cancer progression and associated mortality is cancer invasion, which depends on two key gelatinase enzymes, matrix metalloproteinases-2 and -9. dried longan ( euphoria longana lam.) seed is a rich natural source of antioxidant polyphenols.this study evaluated the effect of dried longan seeds on colon cancer cell invasion via gelatinase function and expression. three dried longan seed fractions were collected by sephadex lh-20 column chromatography. they showed a ...201323527961
effects of encapsulated lactobacillus acidophilus along with pasteurized longan juice on the colon microbiota residing in a dynamic simulator of the human intestinal microbial ecosystem.the effect of encapsulated lactobacillus acidophilus la5 along with pasteurized longan juice on the colon microbiota was investigated by applying a dynamic model of the human gastrointestinal tract. encapsulated l. acidophilus la5 in pasteurized longan juice or sole encapsulated l. acidophilus la5 exhibited the efficiency of colonizing the colon and enabling the growth of colon lactobacilli as well as beneficial bifidobacteria but inhibited the growth of fecal coliforms and clostridia. moreover, ...201423546419
effect of floral sources on the antioxidant, antimicrobial, and anti-inflammatory activities of honeys in taiwan.we evaluated the antioxidant, antibacterial, and anti-inflammatory activities of honey made from different floral sources, including the medicinal herb bidens pilosa, fruit trees, dimocarpus longan, litchi chinensis, and citrus maxima, the taiwanese endemic plant aglaia formosana, and a multifloral forest. the total phenolic and flavonoid contents of the honey made from b. pilosa were significantly higher than those of the other honeys. the honey from b. pilosa also had significantly greater sca ...201323561193
comparative analysis reveals dynamic changes in mirnas and their targets and expression during somatic embryogenesis in longan (dimocarpus longan lour.).somatic embryogenesis (se), which resembles zygotic embryogenesis, is an essential component of the process of plant cell differentiation and embryo development. although micrornas (mirnas) are important regulators of many plant develop- mental processes, their roles in se have not been thoroughly investigated. in this study, we used deep-sequencing, computational, and qpcr methods to identify, profile, and describe conserved and novel mirnas involved in longan (dimocarpus longan) se. a total of ...201323593197
structural identification of (1→6)-α-d-glucan, a key responsible for the health benefits of longan, and evaluation of anticancer activity.longan is a delicious subtropical fruit with great health-beneficial effects. it has been utilized for disease prevention and health care since ancient age. to explore the chemicals responsible for the health benefits, water-soluble polysaccharides were extracted from longan flesh in this work. a pure polysaccharide (lps1) was obtained through column purification. analysis by gas chromatography showed lps1 was a homopolysaccharide of glucose with glycosidic linkage of →6)-d-glc-(1→. nuclear magn ...201323617585
efficacy of polymer coating of probiotic beads suspended in pressurized and pasteurized longan juices on the exposure to simulated gastrointestinal environment.alginate-coated lactobacillus acidophilus la5 or lactobacillus casei 01 was recoated with either 0.1-0.5% (w/v) alginate or 0.05-0.15% (w/v) poly-l-lysine (pll) plus 0.2% (w/v) alginate or 5-15% (w/v) gelatin, after which they were determined for survivability in gastric or bile longan juices. the morphology of encapsulated probiotic cells illustrated that recoated beads with 0.5% alginate showed a more compact surface and a greater protective effect than other recoating materials. the recoated ...201323701113
identification of a ptc-containing dlran transcript and its differential expression during somatic embryogenesis in dimocarpus longan.ran (ras-related nuclear protein) plays crucial roles in multiple cellular processes in yeast, animals and plants. here we present a dlran gene and its alternative splicing transcripts containing premature terminator codons (ptcs), identified from embryogenic cultures in longan. multiple alignment and splicing pattern analyses indicated that dlran-1 transcript harboring ptc was the consequence of alternative splicing. the accumulation of dlran ptc-containing transcripts increased significantly w ...201323933275
evaluation of physicochemical and antioxidant properties of sourwood and other malaysian honeys: a comparison with manuka honey.the aim of the present study was to evaluate the physical, biochemical and antioxidant properties of four malaysian monofloral types of honey (gelam, longan, rubber tree and sourwood honeys) compared to manuka honey. several physical parameters of honey, such as ph, moisture content, electrical conductivity (ec), total dissolved solids (tds), color intensity, total sugar and sucrose content, were measured. a number of biochemical and antioxidant tests were performed to determine the antioxidant ...201323938192
morphology and volatile compounds of metathoracic scent gland in tessaratoma papillosa (drury) (hemiptera: tessaratomidae).tessaratoma papillosa (drury) (hemiptera: tessaratomidae) is a serious insect pest of litchi and longan in south china. when disturbed, this insect could release large quantities of disagreeable odorous volatiles from its scent gland. knowledge on the scent gland and its secretion is crucial for developing the semiochemical methods to manage this pest. morphology and ultrastructure of the metathoracic scent glands (mtgs) were studied under stereo and scanning electron microscopy, and the volatil ...201223950062
isolation and characterization of flowering locus t subforms and apetala1 of the subtropical fruit tree dimocarpus longan.longan (dimocarpus longan lour.) is a subtropical evergreen fruit tree, mainly cultivated in asia. two putative floral integrator genes, d. longan flowering locus t1 and 2 (dlft1 and dlft2) were isolated and both translated sequences revealed a high homology to ft sequences from other plants. moreover, two apetala1-like (dlap1-1 and dlap1-2) sequences from longan were isolated and characterized. results indicate that the sequences of these genes are highly conserved, suggesting functions in the ...201323954797
analysis of the global transcriptome of longan (dimocarpus longan lour.) embryogenic callus using illumina paired-end sequencing.longan is a tropical/subtropical fruit tree of great economic importance in southeast asia. progress in understanding molecular mechanisms of longan embryogenesis, which is the primary influence on fruit quality and yield, is slowed by lack of transcriptomic and genomic information. illumina second generation sequencing, which is suitable for generating enormous numbers of transcript sequences that can be used for functional genomic analysis of longan.201323957614
solution properties and in vitro anti-tumor activities of polysaccharides from longan pulp.the solution properties of four fractions (lpi-iv) from crude longan pulp polysaccharides (lp3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with congo red, and atomic force microscopy. their radii of gyration <s²>(z)(½) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <s²>(z)(½) = k m(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 ml/g, respectively. moreover, the depen ...201324051475
metal-organic framework-199/graphite oxide hybrid composites coated solid-phase microextraction fibers coupled with gas chromatography for determination of organochlorine pesticides from complicated samples.the hybrid material of a copper-based metal-organic framework (mof-199) and graphite oxide (go) was explored as the solid-phase microextraction (spme) coating for the first time. this fiber was fabricated by using 3-amino-propyltriethoxysilane (aptes) as the cross-linking agent, which enhanced its durability and allowed more than 140 replicate extractions. with the incorporation of go, the mof-199/go fibers with go contents ranging from 5 to 15 wt% exhibited enhanced adsorption affinity to organ ...201324054558
molecular cloning and development of rapd-scar markers for dimocarpus longan variety authentication.as an edible fruit and source of traditional medicine, d. longan is grown in most areas of southern china. identification of d. longan cultivars by using molecular markers is important genetically. in this study, we cloned fragments from improved randomly amplified polymorphic dna (rapd), and developed stably diagnostic sequence-characterized amplified region (scar) markers. the specific rapd bands of d. longan cultivars from guangxi, with size ranging from 500 bp to 900 bp were gel-purified, cl ...201324130961
modification of pectin and hemicellulose polysaccharides in relation to aril breakdown of harvested longan fruit.to investigate the modification of cell wall polysaccharides in relation to aril breakdown in harvested longan fruit, three pectin fractions (wsp, water soluble pectin; csp, cdta-soluble pectin; asp, alkali soluble pectin) and one hemicellulose fraction (4 m koh-shc, 4 m koh-soluble hemicellulose) were extracted, and their contents, monosaccharide compositions and molecular weights were evaluated. as aril breakdown intensified, csp content increased while asp and 4 m koh-shc contents decreased, ...201324287911
a novel macromolecular extract screened from satsuma with pro-inflammatory effect.excessive consumption of horticultural fruit is a double-edged sword with both positive and negative effects. in eastern countries, a large number of people have suffered from shang huo as a result of excessive consumption of "heating" foods, such as lychee, longan, mandarin orange, mango and civet durian. the present study adopted a step by step strategy screened the compositions with pro-inflammatory effect in satsuma fruits. the pro-inflammatory effects of all fractions were evaluated in raw ...201424336758
effect of microwave power on kinetics and characteristics of microwave vacuum-dried longan (dimocarpus longan lour.) pulp.the drying kinetics of longan (dimocarpus longan lour.) pulp processed by microwave vacuum under different microwave levels (2.67, 5.33, 8.00, and 10.67 w/g) was investigated (pressure controlled at -85 ± 2 kpa) in the present study. it was found that the drying rate of longan pulp was dependent on the microwave power, and the rehydration rate increased from 1.96 to 2.17 with the increase of microwave power from 2.67 to 10.67 w/g. among nine selected thin layer models, the microwave vacuum dryin ...201524367023
[effects of exogenous nitric oxide on physiological characteristics of longan (dimocarpus longana) seedlings under acid rain stress].this paper studied the effects of exogenous nitric oxide donor sodium nitroprusside (snp) on the chlorophyll content, antioxidant enzyme activities, and osmotic regulation substances of longan (dimocarpus longana 'fuyan') seedlings under acid rain (ph 3.0) stress. under the acid rain stress, the seedling leaf superoxide dismutase (sod), peroxidase (pod) and catalase (cat) activities and chlorophyll, soluble protein and soluble sugar contents decreased obviously, while the leaf malondialdedyde co ...201324380343
molecular screening and predation evaluation of the key predators of conopomorpha sinensis bradley (lepidoptera: gracilariidae) in litchi orchards.conopomorpha sinensis bradley (lepidoptera: gracilariidae) is one of the major fruit borer pests of litchi and longan in southern china. although chemical control is effective, alternative, biorational strategies are preferable, and should be developed. predators play an important role in the biological control of agricultural pests, but an accurate method for the evaluation of predation in agriculture has not yet been developed. here, we report a new, specific primer pair to amplify a c. sinens ...201424401157
potential roles of longan flower and seed extracts for anti-cancer.polyphenol-rich plants are known to possess benefits to human health. recent studies have revealed that many traditional chinese medicines (tcms) are rich sources of polyphenols and exhibit antioxidant and anti-inflammatory activities, and these tcms have been shown experimentally to overcome some chronic diseases, including cancer. longan flowers and seeds, two tcms traditionally used for relieving pain and urinary diseases, have been revealed in our recent reports and other studies to possess ...201224520538
antimicrobial activities of various fractions of longan (dimocarpus longan lour. fen ke) seed extract.the antimicrobial activities of longan (dimocarpus longan lour. fen ke) seed extracts were investigated using a disc diffusion method and also determining the minimal inhibitory concentration. the dl-p01-si01 fraction showed that the strongest activity against staphylococcus aureus and methicillin-resistant s. aureus at mic 64 μg/ml, which was found to be due to the phenolic compounds. the hplc analysis showed that the major phenolic compounds are gallic acid, corilagin, ethyl gallate and ellagi ...201424533783
cloning and molecular characterization of phospholipase d (pld) delta gene from longan (dimocarpus longan lour.).longan (dimocarpus longan lour.) is a non-climacteric fruit with a short postharvest life. the regulation of phospholipase d (pld) activity closely relates to postharvest browning and senescence of longan fruit. in this study, a novel cdna clone of longan pldδ (lgpldδ) was obtained and registered in genbank (accession no. jf791814). the deduced amino acid sequence possessed all of the three typical domains of plant plds, a c2 domain and two catalytic hxkxxxxd motifs. the tertiary structure of lg ...201424590739
genetic characterization and authentication of dimocarpus longan lour. using an improved rapd technique.dimocarpus longan lour. is an edible and traditional herb in china, commonly referred to as longon. an improved randomly amplified polymorphic dna (rapd) protocol was here developed in order to determine the geographical origins of d. longan samples collected from 5 provinces in the southern and southwestern areas of china, including sichuan, hainan, fujian, guangdong, and guangxi. generally, the improved rapd method generated good fingerprinting of the 5 samples using the selected 17 primers. i ...201424634243
structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from dimocarpus longan pulp.a new water-soluble polysaccharide (longan polysaccharide 1 (lp1)) was extracted and successfully purified from dimocarpus longan pulp via diethylaminoethyl (deae)-cellulose anion-exchange and sephacryl s-300 hr gel chromatography. the chemical structure was determined using infrared (ir), gas chromatography (gc) and nuclear magnetic resonance (nmr) analysis. the results indicated that the molecular weight of the sample was 1.1 × 10(5) da. monosaccharide composition analysis revealed that lp1 wa ...201424663085
sulfated modification of longan polysaccharide and its immunomodulatory and antitumor activity in vitro.a water-soluble polysaccharide fraction (lp1) was prepared from dimocarpus longan lour. by hot water extraction, deae-cellulose and sephadex g-100 chromatography. its sulfated derivative (lp1-s) was prepared by the sulfuric acid method. preliminary tests in vitro showed lp1 and lp1-s could stimulate murine lymphocytes proliferation, increase pinocytic activity of murine macrophages and production of nitric oxide (no), interleukin 6 (il-6), il-1β and tumor necrosis factor-alpha (tnf-α) in macroph ...201424680807
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