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[contribution of the pcr assay to the diagnosis of mansonella ozzardi in endemic areas of argentina].mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all latin american countries. it has been described along the argentine yungas region. the microscopic diagnosis can yield false-negative test results at low microfilaremia levels. the aim of this study was to optimize the molecular diagnostic technique and compare it with the knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of indi ...201622997768
first report of presumed parasitic keratitis in indians from the brazilian amazon.to describe corneal disorders in patients with systemic parasitic disease.200011095056
ict filariasis test in patients harbouring mansonella ozzardi. 200011123834
molecular identification of wolbachia from the filarial nematode mansonella ozzardi.mansonella ozzardi, a filarial parasite of humans in latin america, has been shown to harbour intracellular bacteria not yet identified. here we show that these bacteria, like those of other filarial nematodes, belong to the genus wolbachia (alpha 2 proteobacteria; rickettsiales). their unambiguous placement in the wolbachia group was shown by 16s rdna sequence analysis. however, the exact position of the wolbachia from m. ozzardi relative to the other wolbachiae is not clear. indeed, 16s rdna s ...200111403387
[hourly variations of mansonella ozzardi microfilaria in the blood]. 195413267095
[length and width of microfilaria of mansonella ozzardi and wuchereria bancrofti in brazil]. 195413267108
[extension of the geographical distribution of mansonella ozzardi in the republic of colombia]. 196314023152
[study of the nyctohemeral variation of "mansonella ozzardi" microfilaria in the blood]. 195914423940
[ocular manifestations in mansonelliasis].to evaluate eye manifestations in mansonelliasis patients from coari, am, rural area on the middle amazonas river.200818516413
molecular identification of wolbachia from the filarial nematode mansonella perstans.wolbachiae are bacterial endosymbionts of insects and many filarial nematodes whose products trigger inflammation in filarial infections. the dependence of the parasites on their endosymbionts has also led to the use of antibiotics directed against the wolbachiae, therapy that has been demonstrated to have a profound salutary effect on filarial infections. the identification of wolbachiae in mansonella species has been conclusively shown for mansonella ozzardi (mo), but not for mansonella persta ...200818538871
current profile of mansonella ozzardi (nematoda: onchocercidae) in communities along the ituxi river, lábrea municipality, amazonas, brazil.the objective of this paper was to describe the current status of mansonella ozzardi prevalence among the inhabitants of 12 communities along the ituxi river, in lábrea municipality, state of amazonas. the prevalence of m. ozzardi was determined using thick blood smears obtained by digital punctures. m. ozzardi was found in 30.23% of the samples collected (39/129), with similar prevalence between genders (males: 30.30%; females: 30.16%); the highest prevalence was found in homemakers (45.45%) fo ...200818660999
improvement of a pcr test to diagnose infection by mansonella ozzardi.mansonelliasis is caused by mansonella ozzardi. it is widespread in the amazon region, with a high prevalence. the common exam of thick blood smears stained with giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia.201121779677
mansonella ozzardi in brazil: prevalence of infection in riverine communities in the purus region, in the state of amazonas.this study was undertaken to investigate the prevalence of mansonella ozzardi infection and to estimate the parasitic infection rate (pir) in simuliid black flies in the municipality of pauini, amazonas, brazil. we used thick blood films to examine 921 individuals in 35 riverine communities along the pauini and purus rivers. simuliids were caught in several communities. flies were identified, stained with haematoxylin and dissected. overall, 44 (24.86%) of 177 riverines were infected in communit ...200919274380
mansonella ozzardi in amazonas, brazil: prevalence and distribution in the municipality of coari, in the middle solimões river.this study investigated some epidemiological aspects of the mansonella ozzardi in municipality of coari, amazonas. clinical symptoms were correlated with the filarial infection and the parasitic infection rates (pir) were estimated in simuliid vectors. the general m. ozzardi human prevalence rate was 13.3% (231/1733), of which 10.2% (109/1069) were from the urban area and 18.4% (122/664) from the rural area. the prevalence rates were higher in men (14.5% urban and 19.7% rural) than in women (6.7 ...201020512236
ivermectin-related adverse clinical events in patients treated for mansonella ozzardi infections.we report the occurrence of serious reactions after treatment with oral ivermectin in two patients with mansonella ozzardi infections. both had systemic and respiratory symptoms and recovered without sequelae. follow-up revealed clearance of microfilaremia in both cases, with relapse in one of them. these reactions are well described in the treatment of other filarial infections, but have not yet been reported in the treatment of m. ozzardi. we are now reporting the first such known reactions wi ...201121491068
a field trial of a pcr-based mansonella ozzardi diagnosis assay detects high-levels of submicroscopic m. ozzardi infections in both venous blood samples and fta card dried blood spots.mansonella ozzardi is a poorly understood human filarial parasite with a broad distribution throughout latin america. most of what is known about its parasitism has come from epidemiological studies that have estimated parasite incidence using light microscopy. light microscopy can, however, miss lighter, submicroscopic, infections. in this study we have compared m. ozzardi incidence estimates made using light microscopy, with estimates made using pcr.201525990611
investigation of the occurrence of mansonella ozzardi in the state of rondônia, western amazonia, brazil.mansonella ozzardi, a human filarial nematode transmitted by diptera simuliidae flies (black flies), is widely spread in the amazon, while the occurrence of mansonelliasis in the state of rondônia (western amazonia) has not been reported thus far. the existence of this parasitic disease in this state is investigated in this study.201121877064
genetic characterization of atypical mansonella (mansonella) ozzardi microfilariae in human blood samples from northeastern peru.dna sequence comparisons are useful for characterizing proposed new parasite species or strains. microfilariae with an atypical arrangement of nuclei behind the cephalic space have been recently described in human blood samples from the amazon region of peru. three blood specimens containing atypical microfilariae were genetically characterized using three dna markers (5s ribosomal dna, 12s ribosomal dna, and cytochrome oxidase i). all atypical microfilariae were clustered into the mansonella gr ...201222826497
mansonelliasis, a neglected parasitic disease in haiti.reported in haiti as early as 1923, mansonella ozzardi is still a neglected disease ignored by the health authorities of the country. this review is an update on the geographic distribution of the coastal foci of mansonelliasis in haiti, the epidemiological profile and prevalence rates of microfilariae in people living in endemic areas, the clinical impact of the parasite on health and the efficiency of the transmission of the parasite among three culicoides biting-midge species identified as ve ...025317697
epidemiologic survey of mansonella ozzardi in corail, haiti.an epidemiologic (cross-sectional study) survey on 462 inhabitants in corail, haiti showed that 16.5% were infected with mansonella ozzardi. this finding was determined from a single 20-μl sample of finger prick blood from each person. among children, < 2% had a detectable microfilaremia. in persons > 15 years of age, the prevalence of infection for males and females was 23% and 21%, respectively. in general, the microfilaremias were low and 70% of positive persons had < 10 microfilariae per 20 ...201424710617
molecular verification of new world mansonella perstans parasitemias.we obtained ribosomal and mitochondrial dna sequences from residents of amazonas state, brazil, with mansonella parasitemias. phylogenetic analysis of these sequences confirm that m. ozzardi and m. perstans parasites occur in sympatry and reveal the close relationship between m. perstans in africa and brazil, providing insights into the parasite's new world origins.201728221120
atypical mansonella ozzardi microfilariae from an endemic area of brazilian amazonia.mansonellosis is endemic in several regions of africa, the caribbean, and latin america. mansonella ozzardi and mansonella perstans have been reported in latin america, including the amazon region. a morphological and molecular microfilariae study was performed in pauini (brazil). blood samples were collected from 40 individuals, and were analyzed by giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm pro ...201627402517
mansonella ozzardi: a neglected new world filarial nematode.mansonella ozzardi (nematoda: onchocercidae) is an understudied filarial nematode, originally described by patrick manson in 1897, that can be transmitted by two families of dipteran vectors, biting midges (most of them members of the genus culicoides) and black flies (genus simulium). with a patchy geographic distribution from southern mexico to northwestern argentina, human infection with m. ozzardi is highly prevalent in some of the caribbean islands, along riverine communities in the amazon ...201627376501
occurrence of mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of lábrea in the western brazilian amazon.introduction mansonella ozzardi is a widely distributed filaria worm in the amazon region. this study aimed to determine the prevalence of m. ozzardi infection in riverine communities of lábrea municipality, amazonas state, brazil. methods a diagnostic blood filtration method in a polycarbonate membrane was used. results m. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p<0.001). the prevalence was higher in longer-term residents ...201627163575
historic accounts of mansonella parasitaemias in the south pacific and their relevance to lymphatic filariasis elimination efforts today.there are two species of filarial parasites with sheathless microfilariae known to commonly cause parasitaemias in humans: mansonella perstans and mansonella ozzardi. in most contemporary accounts of the distribution of these parasites, neither is usually considered to occur anywhere in the eastern hemisphere. however, sir patrick manson, who first described both parasite species, recorded the existence of sheathless sharp-tailed mansonella ozzardi-like parasites occurring in the blood of native ...201626972389
mansonella ozzardi in neotropical region of argentina: prevalence through time (1986-2010).human filariasis caused by mansonella ozzardi is a parasitic infection of a controversial pathology and poorly studied in argentina. the aim of this study was not only to obtain data of the prevalence of m. ozzardi in northwestern argentina through comparison of infection rates in relation to sex and age group determining the range of distribution of mansonelliasis in the region but also to investigate the prevalence of the cases over time. through field work carried between 1986 and 2010 by tec ...201626433075
[mansonelosis by mansonella ozzardi in volunteers undergoing screening for malaria in the peruvian amazon].to determine the frequency, clinical features, and factors associated with m. ozzardi infection in volunteers of a malaria screening campaign in the district of alto nanay in the amazon jungle of peru.201526338385
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