Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| differential expression of an hsp70 gene during transition from the mycelial to the infective yeast form of the human pathogenic fungus paracoccidioides brasiliensis. | we have isolated and characterized cdna and genomic clones that encode a 70 kda heat shock protein (hsp70) from the dimorphic human pathogenic fungus paracoccidioides brasiliensis. the gene encodes a 649-amino-acid protein showing high identity with other members of the hsp70 gene family. the hsp70 gene is induced during both heat shock of yeast cells at 42 degrees c and the mycelial to yeast transition. a differential expression of this gene can be observed between mycelial and yeast forms, wit ... | 1999 | 10096073 |
| fungistatic and fungicidal activities of murine polymorphonuclear leucocytes against yeast cells of paracoccidioides brasiliensis. | recently, a novel culture medium for detecting live yeast cells of paracoccidioides brasiliensis was developed by kurita et al. using this culture medium, murine peritoneal polymorphonuclear leucocytes (pmn) were examined for fungistatic and fungicidal activities against p. brasiliensis yeast cells. the magnitude of the antifungal effect of pmn varied depending upon the fungal isolates used. pmn exhibited a killing effect on p. brasiliensis isolate bt-4 in 2 h of coculture. in contrast, the othe ... | 1999 | 10200930 |
| serodiagnosis of histoplasmosis, paracoccidioidomycosis and penicilliosis marneffei; current status and future trends. | effective serodiagnosis of systemic fungal infections is of increasing importance, particularly with regard to the identification of infection with histoplasma capsulatum, paracoccidioides brasiliensis and penicillium marneffei. methodology has been based either around antibody or antigen detection, although there is clear overlap between the two. antibody-based detection systems for the diagnosis of histoplasmosis, paracoccidioidomycosis and penicilliosis marneffei have now begun to incorporate ... | 1998 | 10206744 |
| involvement of the major glycoprotein (gp43) of paracoccidioides brasiliensis in attachment to macrophages. | the yeast form of paracoccidioides brasiliensis, the causative agent of a deep mycosis in humans, is known to be phagocytized by, and to multiply inside, macrophages. in this work we describe the involvement of gp43, a major antigenic protein of p. brasiliensis, in the initial steps of attachment of the fungus to macrophages. anti-gp43 f(ab) polyclonal fragments were capable of inhibiting phagocytosis in a concentration-dependent manner. sheep red blood cells sensitized with purified gp43 were m ... | 1998 | 10206751 |
| antigenic relationship between loboa loboi and paracoccidioides brasiliensis as shown by serological methods. | lobomycosis and paracoccidioidomycosis are two different mycoses caused by loboa loboi and paracoccidioides brasiliensis, respectively. to verify cross-antigenicity between them, lobomycosis sera were tested by immunoblotting, elisa and capture-eia against crude exo-antigen, 'cell-free antigen' and gp43 from p. brasiliensis. the majority of lobomycosis serum samples recognized crude exo-antigens and gp43 from p. brasiliensis. gp43 was eluted from an affinity column prepared with igg from a patie ... | 1998 | 10206752 |
| lacazia loboi gen. nov., comb. nov., the etiologic agent of lobomycosis. | the new genus lacazia p. taborda, v. taborda, et mcginnis is proposed to accommodate lacazia loboi (o. m. fonseca et lacaz) p. taborda, v. taborda, et mcginnis, the obligate pathogen that causes lobomycosis in mammals. the continued placement of that fungus in the genus paracoccidioides almeida as paracoccidioides loboi is taxonomically inappropriate. loboa loboi ciferri et al. is a synonym of paracoccidioides brasiliensis. | 1999 | 10325371 |
| "giant" blastoconidia of candida albicans: morphologic presentation and concepts regarding their production. | candida albicans normally produces blastoconidia measuring 2 to 8 microns in diameter. markedly enlarged "giant" (approximately 30 microns) blastoconidia of a c. albicans isolate (designated bh) were observed after growth on commercially prepared chocolate agar already supplemented with isovitalex (bbl-microbiology systems, cockeysville, md, usa). morphologically, "giant" blastoconidia presented a spectrum of forms such as blastoconidia with linear creases, with a single broad-based bud resembli ... | 1999 | 10342104 |
| failure of amphotericin b colloidal dispersion in the treatment of paracoccidioidomycosis. | although amphotericin b desoxycholate is considered the most effective treatment for disseminated paracoccidioides brasiliensis infections, little is known about the efficacy of lipid-based formulations of amphotericin b in this infection. in this study, we treated four adults with the juvenile form of paracoccidioidomycosis with 3 mg/kg/day of amphotericin b colloidal dispersion for at least 28 days. although all of the patients initially responded by clinical observation, all four patients rel ... | 1999 | 10344661 |
| comparison between human and armadillo paracoccidioides brasiliensis isolates by random amplified polymorphic dna analysis. | sixty-three paracoccidioides brasiliensis isolates obtained from three nine-banded armadillos (dasypus novemcinctus), one amazonian armadillo's and 19 clinical isolates were compared by random amplified polymorphic dna analysis with the primer opg-19. the isolates were divided into three major clusters, i, ii and iii. coincidences between human and armadillo isolates were observed in clusters i and ii. cluster iii consisted only of armadillos' isolates. the results suggested that (i) humans may ... | 1998 | 10353214 |
| characterization of sphingolipids from mycopathogens: factors correlating with expression of 2-hydroxy fatty acyl (e)-delta 3-unsaturation in cerebrosides of paracoccidioides brasiliensis and aspergillus fumigatus. | significant differences exist between mammals and fungi with respect to glycosphingolipid (gsl) structure and biosynthesis. thus, these compounds, as well as the cellular machinery regulating their expression, have considerable potential as targets for the diagnosis and treatment of fungal diseases. in this study, the major neutral gsl components extracted from both yeast and mycelium forms of the thermally dimorphic mycopathogen paracoccidioides brasiliensis were purified and characterized by 1 ... | 1999 | 10353841 |
| histopathological evolution of experimental paracoccidioidomycosis in wistar rats. | twenty wistar rats were inoculated, by the intracardiac route, with 0.5 ml each of a yeast phase suspension of paracoccidioides brasiliensis utero strain. the rats were sacrificed at regular intervals post-infection, at which time their lungs, heart, liver, spleen and kidneys were removed, fixed and stained for study. the parameters of interest for the lung specimens were: (a) extent of the lesions; (b) number of fungi; (c) presence of a lymphomononuclear halo. extrapulmonary lesions were also s ... | 1999 | 10360321 |
| identification, n-terminal region sequencing and similarity analysis of differentially expressed proteins in paracoccidioides brasiliensis. | paracoccidioides brasiliensis is the causal agent of paracoccidioidomycosis, which is a systemic mycosis in latin america. this human pathogen is a dimorphic fungus existing as mycelium (26 degrees c) and in infected tissues as a yeast form (36 degrees c). the in vitro differentiation process is reversible and dependent on temperature shift. in the present study, the total proteins from both forms of p. brasiliensis (isolate pb01) were analysed by two-dimensional electrophoresis. differentially ... | 1999 | 10361267 |
| ultrastructural characterization of the agent of systemic yeast infection of owl monkeys. | systemic infection by an unclassified yeast-like organism has been encountered sporadically in wild-caught owl monkeys (aotus sp.) from south america. the infection is presumably acquired in the wild; the incubation period ranges from months to years. the disease is indolent and clinical signs are non-specific. the diagnosis is based on histopathologic observation of yeast-like cells in multiple internal organs. most cells appear to be phagocytized by macrophages, however, many are apparently fr ... | 1999 | 10361271 |
| [usefulness of routine investigation of fungal infections through bronchoscopy in hiv-infected and non-hiv-infected patients in a general hospital, reference to aids]. | the diagnostic yields and the spectrum of pulmonary fungal-infection obtained in samples collected by fiberoptic bronchoscopy from hiv-positive and hiv-negative patients were evaluated from 1990 to 1995. a total of 1943 bronchoscopies were performed during this period, 47% in the hiv-positive group and 53% in the hiv-negative group. of 908 hiv-positive patients, 38 (4%) had a fungus isolated from the pulmonary sample whereas of 1035 hiv-negative patients, only 4 (0.2%) had a fungus isolated. his ... | 1999 | 10380564 |
| detection of the basement membrane-degrading proteolytic activity of paracoccidioides brasiliensis after sds-page using agarose overlays containing abz-mkaltlq-eddnp. | we have characterized, in the paracoccidioides brasiliensis yeast phase, an exocellular sh-dependent serine proteinase activity against abz-mkrltl-eddnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. in the present study, we separated the components of p. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by ... | 1999 | 10412577 |
| paracoccidioides brasiliensis. a mycologic and immunochemical study of two strains. | the authors conducted a mycologic, immunochemical and molecular biology study on two strains of paracoccidioides brasiliensis, one of them, called ibia, isolated from soil in the municipality of ibia (minas gerais) by silva-vergara et al. (1996, 1998), and the other, bat, cultivated from a human case of paracoccidioidomycosis in ribeirão preto (são paulo/brazil) by freitas da silva (1996). both strains showed cotton-like (m) and yeast-like (y) forms and were pathogenic for testicularly inoculate ... | 1999 | 10413954 |
| in situ hybridization in paracoccidioidomycosis. | in situ hybridization (ish) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (act ccc ccg tgg tc) and its complementary sequence. when combining ish with the gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. when the complementary probe was used, labelling ... | 1999 | 10421853 |
| electrophoretic karyotype of environmental isolates of paracoccidioides brasiliensis. | five of the 12 environmental isolates of the dimorphic fungus paracoccidioides brasiliensis known to date, were analysed by contour-clamped homogeneous electric field gel electrophoresis (chef). the electrophoretic pattern was shown to consist of five bands, with molecular size ranging from 3.2 to 10 mb, a model quite similar to the one found in the clinical isolates previously tested and used here as controls. however, one of the bands in the environmental isolates had a lesser weight (7.2 mb), ... | 1999 | 10421855 |
| antifungal activity of human polymorphonuclear leucocytes against yeast cells of paracoccidioides brasiliensis. | human peripheral blood polymorphonuclear leucocytes (pmn) were examined in vitro for antifungal activity against yeast cells of paracoccidioides brasiliensis. the yeast cell of this fungus was resistant to killing by pmn. however, pmn exhibited a fungistatic effect on the fungal isolates employed in a long-term ( approximately 72 h) assay. lysates of pmn did not show a fungistatic or fungicidal effect, indicating that live pmn are necessary for the antifungal effect. interferon-gamma (ifn-gamma) ... | 1999 | 10421861 |
| virulence of paracoccidioides brasiliensis isolates can be correlated to groups defined by random amplified polymorphic dna analysis. | fifteen paracoccidioides brasiliensis isolates were discriminated by the rapd analysis into two groups with only 17% of genomic identity. the ability of p. brasiliensis isolates to invade tissues was studied in an experimental model using susceptible b10.a mice. the analysis was performed according to the severity of the lesions including the number and size of the granuloma, the number and dissemination of fungi to different organs. the isolates from two rapd groups demonstrated a marked differ ... | 1999 | 10421862 |
| correlation between antigenemia of paracoccidioides brasiliensis and inhibiting effects of plasma in patients with paracoccidioidomycosis. | metabolites produced by pathogenic fungi may be involved in the pathogenesis of fungal infections consequently altering the defence mechanisms of the host. in this study the levels of paracoccidioides brasiliensis antigens detected in the plasma of patients with paracoccidioidomycosis correlated with the suppression index detected by the low mitogenic response of peripheral blood mononuclear cells (pbmc) to phytohaemaglutinin (pha). this inhibitory effect on lymphoproliferation was observed in t ... | 1999 | 10421863 |
| ultrastructural differences observed in paracoccidioides brasiliensis yeast phase cells grown on solid and in liquid medium. | an ultrastructural study was conducted on, yeast-like paracoccidioides brasiliensis cells grown on liquid and solid peptone--yeast extract--glucose medium. a large proportion of cells grown in liquid medium presented cytoplasmic damage compared with the cells grown on solid medium, which remained intact, suggesting that agar plays an important role in the development of this fungus. | 1999 | 10424097 |
| [in vitro sensitivity of paracoccidioides brasiliensis to systemically used antifungal agents]. | in 1992, the nccls proposed a broth macrodilution method for determining the in vitro susceptibility of yeast. however, for dimorphic fungi no standardised method is available. the aim of our study was to evaluate the reference broth macrodilution method with isolates of paracoccidioides brasiliensis. the in vitro susceptibility of two atcc strains (32069 and 36324) and nine clinical isolates were determined against serial dilutions of amphotericin b (amb), ketoconazole (ktz), and itraconazole ( ... | 1999 | 10425663 |
| paracoccidioidomycosis and aids: report of the first two colombian cases. | the records of the first two colombian patients with aids and paracoccidioidomycosis are presented. both patients were males and had no known risk factors for hiv although in the past they had worked in the field where they could have been infected with the fungus. they exhibited the juvenile type of disease with multiple organ system involvement and symptoms of short duration. they were deeply immunodepressed as indicated by less than 100 cd4 t lymphocytes per ml; however, serologic tests revea ... | 1998 | 10436658 |
| constitutive melanin in the cell wall of the etiologic agent of lobo's disease. | lobo's disease is a chronic granulomatous disease caused by the obligate pathogenic fungus, whose cell walls contain constitutive melanin. in contrast, melanin does not occur in the cell walls of paracoccidioides brasiliensis when stained by the fontana-masson stain. | 1999 | 10436664 |
| paracoccidioidomycosis. | paracoccidioidomycosis is a systemic infection caused by the dimorphic fungus paracoccidioides brasiliensis. it is commonly an endemic disease in latin america, but several cases have been reported outside this area, particularly now in this time of world globalization. primary pulmonary infection occurs commonly in the first and second decades of life and usually has a benign, self-limited respiratory infection course. the adult chronic manifestation of the disease is usually the result of reac ... | 1999 | 10461538 |
| macrophage expression of class ii major histocompatibility complex gene products in paracoccidioides brasiliensis-infected mice. | c57b1/6 isogenic mice infected with paracoccidioides brasiliensis strains showed a disruption in the expression of ia antigen. expression slowly decreased during the course of the infection with a slight variation dependent on the route of inoculation and the fungal strain used, but production of interferon-gamma and tumor necrosis factor-alpha were observed. suppression of ia antigen expression and depression of the immunoproliferative responses of spleen cells were strongly correlated with nit ... | 1999 | 10463680 |
| [paracoccidioides brasiliensis infection in a subtropical region with important environmental changes]. | paracoccidioidomycosis is a systemic mycoses caused by paracoccidioides brasiliensis, a dimorphic fungus that infects man through respiratory ducts. it has been accepted that its ecological niche is located in the soil and plants of subtropical forests of latin america. the province of corrientes is located at the northeastern border of argentina, in a subtropical area where important environmental modifications have been introduced in the last decade as consequence of damming the paranà river a ... | 1999 | 10472444 |
| pathogenicities and gp43kda gene of three paracoccidioides brasiliensis isolates originated from a nine-banded armadillo (dasypus novemcinctus). | we studied three different isolates of paracoccidioides brasiliensis obtained from the mesenteric lymph node (d3ly1), the spleen (d3s1) and the liver (d3liv1) of the same armadillo (dasypus novemcinctus). pulmonal inflammatory area was evaluated by intravenous inoculation of 10(6) yeast cells of each isolates in young, male, ddy mice. moreover, the partial sequence of gp43kda gene of p. brasiliensis was analyzed. the lung inflammatory area was greater in animals inoculated with isolate d3s1. the ... | 1998 | 10481285 |
| role of the armadillo dasypus novemcinctus in the epidemiology of paracoccidioidomycosis. | a study conducted in a rural area of ibiá, state of minas gerais, brazil, where paracoccidioides brasiliensis was recently isolated from soil, sought to determine if the armadillo dasypus novemcinctus developed paracoccidioidomycosis. out of 21 armadillos captured in the area, one had a lung granuloma containing fungal cells attributable to those of p. brasiliensis. the present report presents the first histopathological evidence for the presence of this pathogen in the lungs of an armadillo. th ... | 1998 | 10531678 |
| subcutaneous infection of mice with paracoccidiodes brasiliensis induces a peculiar pattern of inflammatory and immune responses. | it is well established that resistance or susceptibility to paracoccidioidis brasiliensis infection in mice is under strict host's genetic control. mice from a/sn strain inoculated by the ip route are resistant to fungal infection while infection induced in mice from b10.a strain results in a fatal disease. the early cellular events of infection in both strains are characterized by a marked neutrophilic infiltration that is more prominent in b10.a mice. a peculiar characteristic of the paracocci ... | 1999 | 10560623 |
| experimental paracoccidioidomycosis in hamsters (mesocricetus auratus): gestational interactions. | paracoccidioides brasiliensis is a dimorphic fungus presenting specific steroid hormone receptors, both in the yeast and mycelial forms and estrogen inhibits the transition from mycelium to yeast. in the acute phase, the disease occurs with equal frequency in both sexes but in adults, females are spared. placental fungal infection has been reported, but references to fetal infection have not been confirmed. we used 78 syrian female hamsters divided into 3 groups: gi consisted of 30 infected mate ... | 1999 | 10598068 |
| paracoccidioides brasilienses isolates obtained from patients with acute and chronic disease exhibit morphological differences after animal passage. | the basis for virulence in paracoccidioides brasiliensis is not completely understood. there is a consensus that the sequential in vitro subcultivation of p. brasiliensis leads to loss of its pathogenicity, which can be reverted by reisolation from animal passage. attention to morphological and biochemical properties that are regained or demonstrated after animal passage may provide new insights into factors related to the pathogenicity and virulence of p. brasiliensis. we evaluated morphologica ... | 1999 | 10602541 |
| differential antibody isotype expression to the major paracoccidioides brasiliensis antigen in juvenile and adult form paracoccidioidomycosis. | we investigated the relationship between antibody response to the major paracoccidioides brasiliensis antigen, a 43-kda glycoprotein, and the two paracoccidioidomycosis (pcm) clinical presentations, the juvenile and the adult forms. total immunoglobulin g (igg), igg isotypes, and iga anti-gp43 antibodies were determined by enzyme-linked immunosorbent assay in patients'sera. juvenile pcm patients had higher (p =.003) igg anti-gp43 levels than adult form patients. igg1 subclass levels, however, we ... | 1999 | 10602660 |
| in vitro human immune reactivity of fast protein liquid chromatography fractionated paracoccidioides brasiliensis soluble antigens. | soluble antigens of paracoccidioides brasiliensis yeast cells (pbag) were fractionated in a fast protein liquid chromatography (fplc) system, using q-sepharose anion-exchange resin, in order to characterize antigenic fractions that could elicit cell reactivity and antibody recognition in human paracoccidioidomycosis (pcm). pbag fractions were eluted by 20 mm tris-hcl solution (ph 9.6) with an increasing gradient up to 1 m nacl. the fplc system was able to resolve 7 fractions, enumerated from 0 t ... | 1999 | 10602668 |
| depletion of cd8(+) t cells in vivo impairs host defense of mice resistant and susceptible to pulmonary paracoccidioidomycosis. | using a pulmonary model of infection, we demonstrated previously that a/sn and b10.a mice are, respectively, resistant and susceptible to paracoccidioides brasiliensis infection. employing the same experimental model, we examined herein the role of cd8(+) t cells in the course of paracoccidioidomycosis. treatment with anti-cd8 monoclonal antibodies caused a selective depletion of pulmonary and splenic cd8(+) t cells in both mouse strains. the number of pulmonary cd4(+) t cells and immunoglobulin ... | 2000 | 10603408 |
| mri of head and neck paracoccidioidomycosis. | paracoccidioidomycosis, caused by the dimorphic fungus paracoccidioides brasiliensis, is the most important systemic mycosis in latin america. imported cases have been reported in north america, asia and europe, in individuals who lived in endemic areas, sometimes many years before the development of clinical manifestations. the disease causes cutaneous and/or respiratory tract mucosal lesions as well as lymph node enlargement. involvement of the oropharynx and/or the larynx, either alone or in ... | 1999 | 10624334 |
| resistance to paracoccidioides brasiliensis infection is linked to a preferential th1 immune response, whereas susceptibility is associated with absence of ifn-gamma production. | the secretion of interferon-gamma (ifn-gamma), interleukin-2 (il-2), il-4, il-5, and il-10 by antigen-stimulated lymph node cells, eosinophil maturation, and the antibody isotypes produced were examined during intraperitoneal infection of susceptible (b10.a) and resistant (a/sn) mice with paracoccidioides brasiliensis. lymph node cells from resistant mice produced early and sustained levels of ifn-gamma and il-2, whereas susceptible animals secreted low to undetectable amounts of these type 1 cy ... | 2000 | 10670655 |
| pcr with paracoccidioides brasiliensis specific primers: potential use in ecological studies. | the precise microenvironment of paracoccidioides brasiliensis has not yet been discovered perhaps because the methods used are not sensitive enough. we applied to this purpose the polymerase chain reaction (pcr) using three sets of specific primers corresponding to two p. brasiliensis genes. this fungus as well as several other fungi, were grown and their dna obtained by mechanical disruption and a phenol chloroform isoamylalcohol-based purification method. the dna served for a pcr reaction that ... | 1999 | 10671288 |
| influence of the genetic background on the pattern of lesions developed by resistant and susceptible mice infected with paracoccidioides brasiliensis. | to compare the sequential evolution of lesions developed by resistant (a/sn) and susceptible (b10.a) mice to paracoccidioides brasiliensis infection we inoculated a virulent isolate of the fungus and collected the pancreas/peripancreatic omentum monthly (from 1 to 6 months) post infection. after fixation, tissue sections were stained by conventional methods for light microscopy to investigate the cellular composition, the extracellular matrix (ecm) patterns and the morphology of the yeasts in th ... | 1999 | 10691092 |
| molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungus paracoccidioides brasiliensis. | 1,3-beta-d-glucan is a fungal cell wall polymer synthesized by the multi-subunit enzyme 1,3-beta-d-glucan synthase. a subunit of this integral membrane protein was first described as the product of the fks1 gene from saccharomyces cerevisiae using echinocandin mutants. other fks1 genes were also reported for candida albicans, aspergillus nidulans and cryptococcus neoformans. here, we report the nucleotide sequence of the first homologous fks gene cloned from the pathogenic fungus paracoccidioide ... | 2000 | 10705373 |
| production of proteinase and phospholipase by paracoccidioides brasiliensis. | we have investigated the production of proteinase and phospholipase by 20 different isolates of paracoccidioides brasiliensis. isolates were grown in bacto-peptone, dextrose, ph 5.5, agar slants, at 27 degrees c for 30 days, and cultures were transferred onto petri dishes containing basis medium and bovine serum albumin fraction v and sterile egg yolk as substrates for enzyme production, and incubated at 27 degrees c. after 30 days net enzyme activity was visualized and quantitatively evaluated, ... | 1999 | 10721516 |
| comparative morphology of lacazia loboi (syn. loboa loboi) in dolphins and humans. | lacazia loboi (syn. loboa loboi), the etiological agent of lobomycosis, was compared in human and dolphin tissue using light and electron microscopy, and computer-assisted morphometrics. the histological features of the lesions were similar; however, preliminary electron microscopy data indicates that cell wall destruction may vary in the two hosts. calcofluor stained tissue sections of human and dolphin tissue were examined with uv light microscopy and the images digitized. measurements of area ... | 2000 | 10746221 |
| differential expression of chitin synthase genes during temperature-induced dimorphic transitions in paracoccidioides brasiliensis. | fragments of five genes encoding chitin synthase enzymes were identified in the dimorphic fungus paracoccidioides brasiliensis by polymerase chain reaction (pcr) amplification of conserved chs gene domains. these represent several classes of enzyme: pbrchs1, class i; pbrchs2, class ii; pbrchs3, class iv; and pbrchs4 and pbrchs5, class v. expression of these genes during the temperature regulated dimorphic transition from yeast to mycelium and from mycelium to yeast was determined by northern ana ... | 2000 | 10746225 |
| experimental paracoccidioidomycosis of the syrian hamster: fungicidal activity and production of inflammatory cytokines by macrophages. | phagocytic cells play an important role in nonspecific resistance to fungal infection by mediating an inflammatory response and by a direct fungicidal action. in this study, the functional activity of peritoneal macrophages obtained from hamsters experimentally infected with strain pb18 of paracoccidioides brasiliensis was evaluated during 16 weeks of infection. the results showed that macrophages had a higher spreading ability associated with increased production of tumor necrosis factor alpha ... | 2000 | 10746228 |
| nitric oxide participation in the fungicidal mechanism of gamma interferon-activated murine macrophages against paracoccidioides brasiliensis conidia. | paracoccidioidomycosis, a systemic mycosis restricted to latin america and produced by the dimorphic fungus paracoccidioides brasiliensis, is probably acquired by inhalation of conidia produced by the mycelial form. the macrophage (mphi) represents the major cell defense against this pathogen; when activated with gamma interferon (ifn-gamma), murine mphis kill the fungus by an oxygen-independent mechanism. our goal was to determine the role of nitric oxide in the fungicidal effect of mphis on p. ... | 2000 | 10768942 |
| comparative analysis of ceramide structural modification found in fungal cerebrosides by electrospray tandem mass spectrometry with low energy collision-induced dissociation of li+ adduct ions. | fungal cerebrosides (monohexosylceramides, or cmhs) exhibit a number of ceramide structural modifications not found in mammalian glycosphingolipids, which present additional challenges for their complete characterization. the use of li+ cationization, in conjunction with electrospray ionization mass spectrometry and low energy collision-induced dissociation tandem mass spectrometry (esi-ms/cid-ms), was found to be particularly effective for detailed structural analysis of complex fungal cmhs, es ... | 2000 | 10775088 |
| a case of paracoccidioidomycosis: experience with long-term therapy. | we describe long-term therapy for paracoccidioidomycosis occurring in a 61-year-old house-painter from venezuela. the diagnostic examinations made in south america had shown pulmonary granulomatous lesions and an osteolytic pattern of the left knee that had been considered suspect of malignant disease with an indication for limb amputation. with the aid of fine needle aspiration biopsy (fnab) and culture examination we diagnosed an osteomyelitis by paracoccidioides brasiliensis and initiated the ... | 2000 | 10782401 |
| interferon-gamma and tumor necrosis factor-alpha determine resistance to paracoccidioides brasiliensis infection in mice. | to investigate the role of interferon-gamma (ifn-gamma) and tumor necrosis factor-alpha (tnf-alpha) in the resistance to paracoccidioides brasiliensis (pb) infection, mice with homologous disruption of the ifn-gamma (gko) or tnf-alpha receptor p55 (p55ko) were infected with the parasite. gko and p55ko, but not wild-type (wt) mice, were unable to control the growth of yeast cells and the mice succumbed to infection by days 16 and 90 after infection, respectively. typical inflammatory granulomas w ... | 2000 | 10793093 |
| fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in balb/c mice infected with viable and non-viable paracoccidioides brasiliensis propagules. | patients with paracoccidioidomycosis often present pulmonary fibrosis and exhibit important respiratory limitations. based on an already established animal model, the contribution of viable and non-viable p. brasiliensis propagules to the development of fibrosis was investigated. balb/c male mice, 4-6 weeks old were inoculated intranasally either with 4x10(6) viable conidia (group i), or 6. 5x10(6) fragmented yeast cells (group ii). control animals received pbs. six mice per period were sacrific ... | 2000 | 10810319 |
| sialic acids in fungi: a minireview. | the increasing number of reports on the presence of sialic acids in fungi (n-acetyl-, n-glycolyl- and 5,9-n,o-diacetylneuraminic acids) based on direct and indirect evidence warrants the present review. formerly suggested as sialidase-sensitive sources of anionic groups at the cell surface of fungal species grown in chemically defined media (e.g., fonsecaea pedrosoi), sialic acids have also been found in sporothrix schenckii, paracoccidioides brasiliensis, cryptococcus neoformans and recently, i ... | 1999 | 10815991 |
| effect of cytokines on antifungal activity of human polymorphonuclear leucocytes against yeast cells of paracoccidioides brasiliensis. | in our previous study, it was observed that human peripheral blood polymorphonuclear leucocytes (pmns) exhibited a fungistatic effect on yeast cells of paracoccidioides brasiliensis, and that interferon-gamma (ifn-gamma), but not tumor necrosis factor-alpha or interleukin-8 (il-8), enhanced the antifungal activity of pmns. in the present study, granulocyte-macrophage colony-stimulating factor (gm-csf) also enhanced the pmn activity. gm-csf-activated pmns exhibited a killing effect on p. brasilie ... | 2000 | 10817235 |
| comparative efficacy of fluconazole and amphotericin b in the parenteral treatment of experimental paracoccidioidomycosis in the rat. | patients with severe and complicated paracoccidioidomycosis are treated with amphotericin b by the intravenous route. fluconazole is active in vitro against paracoccidioides brasiliensis and can also be administered intravenously, but few clinical or experimental data are available about its action against the infection caused by this fungus. in the present study, the efficacy of fluconazole and amphotericin b was assessed comparatively in rats inoculated parenterally with p. brasiliensis. the t ... | 1999 | 10823185 |
| dna-based vaccination against murine paracoccidioidomycosis using the gp43 gene from paracoccidioides brasiliensis. | gp43, the major 43-kda antigenic glycoprotein of paracoccidioides brasiliensis, or its 15-amino acid inner peptide (p10), induces a t-cd4(+), th1 cellular immune response which protects balb/c mice from intratracheal infection by virulent yeast forms. we investigated whether dna vaccination using the gp43 gene could elicit protective immunity against p. brasiliensis. animals immunised intramuscularly (i.m.) or intradermally (i.d.) with plasmid dna containing the gp43 gene induced a specific, lon ... | 2000 | 10825609 |
| effects of amphotericin b and three azole derivatives on the lipids of yeast cells of paracoccidioides brasiliensis. | yeast cells of five different strains of paracoccidioides brasiliensis were obtained for partial analysis of lipid composition, and sterol content was determined quantitatively and qualitatively. the determinations were conducted with cells cultured in the presence and absence of amphotericin b and azole derivatives at levels below the mic. | 2000 | 10858371 |
| antifungal activity of the new azole uk-109, 496 (voriconazole). | the in vitro activity of voriconazole fully includes aspergillus, and also emerging moulds like fusarium, pseudallescheria boydii, and penicillium marneffei. the minimal inhibitory concentrations of voriconazole for candida krusei and candida glabrata, which are resistant or less susceptible to fluconazole, promise clinical efficacy, although they are ten times higher (0.30-0.39 microgram/ml) than those for candida albicans and other candida spp. (0.001-0.05 microgram/ml). the endemic fungal pat ... | 1999 | 10865910 |
| diploid strains of the pathogenic basidiomycete cryptococcus neoformans are thermally dimorphic. | cryptococcus neoformans is an opportunistic human pathogenic fungus with a defined sexual cycle. clinical and environmental isolates of c. neoformans are haploid, and the diploid stage of the lifecycle is thought to be transient and unstable. in contrast, we find that diploid strains are readily obtained following genetic crosses of congenic matalpha and mata strains. at 37 degrees c, the diploid strains grow as yeast cells with a single nucleus that is larger than a haploid nucleus, contains a ... | 2000 | 10882532 |
| [lobomycosis: a mycosis seldom observed in metropolitan france]. | we report a case of lobomycosis presenting like a cutaneous tumoral lesion in a patient living in guyana. the causative agent is loboa loboi. the illness is seldom observed in metropolitan france. the epidemiology, the clinical features, the course and the treatment are studied. the diagnosis is histological, with the morphological features of yeast-like cells. | 2000 | 10891722 |
| a critical analysis of isolation of paracoccidioides brasiliensis from soil. | the present review is a critical analysis of positive and negative reports of the isolation of paracoccidioides brasiliensis from soil. the strains isolated from soil or soil-contaminated material (dogfood, penguin feces) by batista et al. in recife, brazil, negroni in the argentinian chaco, albornoz in rural venezuela, silva-vergara et al. in ibiá, brazil, ferreira et al. in uberlândia, brazil, and gezuele et al. at the uruguayan base in the antarctic region, presented mycological characteristi ... | 2000 | 10892985 |
| isolation of paracoccidioides brasiliensis from armadillos (dasypus novemcinctus) in an area where the fungus was recently isolated from soil. | natural infection of armadillos (dasypus novemcinctus) with paracoccidioides brasiliensis in northern brazil was reported in 1986, raising great interest in the understanding of the role of this mammal in the epidemiological cycle of the fungus. recently, p. brasiliensis was isolated from the soil of ibiá, state of minas gerais, southeastern brazil. armadillos captured in this area were evaluated for the presence of p. brasiliensis in the viscera and infection was detected in 4/16 animals (25%). ... | 2000 | 10892986 |
| production of ifn-gamma is impaired in patients with paracoccidioidomycosis during active disease and is restored after clinical remission. | cellular immunity is usually suppressed during paracoccidioidomycosis (pcm) and is restored after treatment. in this study we evaluated the induction of a type 1 (interferon gamma (ifn-gamma)), a type 2 (interleukin (il)-10) and a primarily macrophage derived cytokine (tumor necrosis factor (tnf)-alpha) in peripheral blood mononuclear cells (pbmc) from patients with pcm. eight male patients with active pcm, nine male patients with clinical remission of the disease and 10 healthy control subjects ... | 2000 | 10892991 |
| comparison of an established antibody sandwich method with an inhibition method of histoplasma capsulatum antigen detection. | the histoplasma antigen immunoassay utilizes an antibody sandwich method that provides a rapid and reliable means of diagnosing the more severe forms of histoplasmosis. inhibition assays have been developed for antigen detection and offer at least one potential advantage, namely, reduced antibody requirements. we have developed an inhibition assay using the polyclonal antibody employed in our standard sandwich assay. urine and serum specimens from patients with culture-proven histoplasmosis and ... | 2000 | 10921949 |
| detection of paracoccidioides brasiliensis in tissue samples by a nested pcr assay. | a nested pcr assay for the detection of paracoccidioides brasiliensis dna was evaluated, using a sequence of the immunogenic gp43 gene as a target. this gene encodes an outer membrane protein unique to this dimorphic fungus. dna from six clinical isolates and the atcc strain 60885 of p. brasiliensis, as well as dna from closely related fungi, was examined to determine detection limits and cross-reactions. pcr was done on dna extracts of lung homogenates from 23 experimentally p. brasiliensis-inf ... | 2000 | 10921955 |
| molecular identification of paracoccidioides brasiliensis by pcr amplification of ribosomal dna. | we have amplified and sequenced the 5.8s and 28s ribosomal dna genes and intergenic regions of paracoccidioides brasiliensis, strain pb01. using primers specifically designed for both ribosomal dna regions, we were able to discriminate between p. brasiliensis and other human pathogenic fungi by pcr. the use of this molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and molecular epidemiological studies of p. brasiliensis in latin america. | 2000 | 10921990 |
| random amplified polymorphic dna (rapd) analysis of paracoccidioides brasiliensis isolates. | five paracoccidioides brasiliensis isolates of humans origin were analyzed using three arbitrary primers (3301, 3304 and 3307 of 10, 9 and 10 oligonucleotídes respectively) in random amplified polymorphic dna (rapd) analysis. the analysis of the complex rapd profiles obtained were carried out using the dice similarity coefficient that distinguished the isolated pb 02 from the others (pb 18, pb 192, pb 265 and pb sn). the results revealed limited intraspecific genomic variations in these p. brasi ... | 1999 | 10932759 |
| adherence and intracellular parasitism of paracoccidioides brasiliensis in vero cells. | paracoccidioides brasiliensis is a dimorphic fungus known to produce invasive systemic disease in humans. the 43-kda glycoprotein of p. brasiliensis is the major diagnostic antigen of paracoccidioidomycosis and may act as a virulence factor, since it is a receptor for laminin.very little is known about early interactions between this fungus and the host cells, so we developed in vitro a model system employing cultured mammalian cells (vero cells), in order to investigate the factors and virulenc ... | 2000 | 10962270 |
| small subunit ribosomal dna sequence shows paracoccidioides brasiliensis closely related to blastomyces dermatitidis. | the similarities of paracoccidioidomycosis and blastomycosis are highly suggestive of a close relation of the two etiological agents. whereas the agent of the first disease is exclusively endemic in latin america, the agent of the latter one is endemic in north america and africa. in symptomatic travelers visiting both areas of endemicity, differentiation of the diseases might be impossible, even though therapy and prognosis for these two diseases differ significantly. in order to identify diffe ... | 2000 | 10970355 |
| pcr for diagnosis of paracoccidioidomycosis. | a pcr assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-da (gp43) antigen was developed to detect paracoccidioides brasiliensis dna in sputa. in the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis. | 2000 | 10970409 |
| [paracoccidioidomycosis of the central nervous system: case report]. | the involvement of the central nervous system in paracoccidioidomycosis is more frequent than previously thought. the first reference to the possibility that paracoccidioides brasiliensis could affect the central nervous system was by pereira & jacobs in 1919. since then, a great number of other studies has showed this form of clinical behavior and, in some of them, the frequency has ranged 27.27%. we report a clinical case of a 34-year-old white brazilian woman admitted because of bacterial pne ... | 2000 | 10973120 |
| experimental paracoccidioidomycosis in high and low antibody responder mice of selection iv-a. | high (h) and low (l) responder mice were selected for their ability to produce antibodies against sheep and human erythrocytes (selection iv-a). in this selection, the difference in antibody responsiveness between h and l lines (hiv-a and liv-a mice, respectively) was shown to depend mainly on macrophage function. the more rapid catabolism of antigens by macrophages in l mice has been suggested as the main cause of the low antibody production. due to this high macrophage activity, l animals have ... | 2000 | 10975699 |
| morphological aspects of paracoccidioides brasiliensis in lymph nodes: implications for the prolonged latency of paracoccidioidomycosis? | in order to determine if fungal morphology in tissues would furnish indications on the viability of paracoccidioides brasiliensis yeast cells, lymph node biopsies from five patients with paracoccidioidomycosis, including one with residual circumscribed lesions, were examined. a program that allows transferring of microscopic images to the computer for further processing was used. in the four active cases, the infected lymph nodes had over 49% of healthy-looking yeast cells while in the case of t ... | 2000 | 10975700 |
| a new pcr primer for the identification of paracoccidioides brasiliensis based on rrna sequences coding the internal transcribed spacers (its) and 5 x 8s regions. | internal transcribed spacer (its) genes including the 5.8s ribosomal (r)rna of paracoccidioides brasiliensis were amplified and the dna sequences were determined. based on a comparison of the sequence information, a new polymerase chain reaction (pcr) primer pair was designed for specific amplification of dna for p. brasiliensis. this primer pair amplified a 418-bp dna sequence and was 100% successful in identifying 29 strains of p. brasiliensis (including the reference strains) isolated from th ... | 2000 | 10975701 |
| [in vitro infection by different strains of paracoccidioides brasiliensis]. | we analyzed the in vitro infection process by p. brasiliensis and the effect of extracellular factor(s) produced on monolayers of mammalian vero cell lines. the yeast phase of four strains was studied: b339 (avirulent or slightly virulent), u, (intermediate virulence), 93745 and 63265 (both highly virulent). strains of intermediate and high virulence had higher adherence at first contact (about 16%). strain b339 had a slower adherence at first contact (8%) than the others during the same period. ... | 2000 | 11008702 |
| the role of hla antigens in the development of paracoccidioidomycosis. | paracoccidioidomycosis is a systemic granulomatous disease that involves primarily the lungs and may disseminate to other organs and systems. it is caused by paracoccidioides brasiliensis, a fungus that exhibits reversible thermal dimorphism and whose natural habitat is presently unknown. there are two main clinical forms: the acute (subacute) juvenile form and the chronic adult form. the former runs a more rapid course and is more severe than the latter. this mycosis is found throughout latin a ... | 2000 | 11032058 |
| paracoccidioidomycosis: a model for evaluation of the effects of human immunodeficiency virus infection on the natural history of endemic tropical diseases. | the interaction of human immunodeficiency virus (hiv) infection with endemic tropical diseases has become a major concern, but its mechanisms are still poorly understood. paracoccidioidomycosis (pcm), a south america endemic deep mycosis, may provide an interesting model to investigate this interaction, as clinical-epidemiological features of most hiv-pcm-coinfected patients are difficult to classify into the standard acute and chronic forms of pcm. such patients have presented clinical features ... | 2000 | 11049788 |
| polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus paracoccidioides brasiliensis. | the gp43 glycoprotein is an immune-dominant antigen in patients with paracoccidioidomycosis (pcm). it is protective against murine pcm and is a putative virulence factor. the gp43 gene of paracoccidioides brasiliensis b-339 is located in a 1,329-bp dna fragment that includes two exons, a 78-bp intron, and a leader peptide-coding region of 105 bp. polymorphism in gp43 has been suggested by the occurrence, in the same isolate or among different fungal samples, of isoforms with distinct isoelectric ... | 2000 | 11060052 |
| correlation among immune response, morphogenesis of the granulomatous reaction and spleen lymphoid structure in murine experimental paracoccidioidomycosis. | we studied the correlation among cellular immune response, the pattern of lung granulomatous lesions and alterations in spleen lymphoid structure in swiss mice inoculated intravenously with paracoccidioides brasiliensis strain 18. the animals were evaluated at 24, 48 and 96 h after infection and further studied weekly for 18 weeks by: (i) the macrophage migration inhibition test with phytohemagglutinin (pha) and p. brasiliensis antigen (pbag); and (ii) histopathology of the lung and spleen lesio ... | 2000 | 11092384 |
| bone paracoccidioidomycosis in an hiv-positive patient. | aids patients are vulnerable to infection by opportunistic microbes, including various fungi such as pneumocystis carinii, cryptococcus neoformans, histoplasma capsulatum, candida albicans and many others. however, the association of aids and infection with paracoccidioides brasiliensis has been rarely recorded. we report a case of an hiv-positive patient with bone infection by this fungus with a clinical form not previously published. this clinical presentation included primarily a massive bone ... | 1997 | 11105147 |
| phylogenetic analysis of lacazia loboi places this previously uncharacterized pathogen within the dimorphic onygenales. | lacazia loboi is the last of the classical fungal pathogens to remain a taxonomic enigma, primarily because it has resisted cultivation and only causes cutaneous and subcutaneous infections in humans and dolphins in the new world tropics. to place it in the evolutionary tree of life, as has been done for the other enigmatic human pathogens pneumocystis carinii and rhinosporidium seeberi, we amplified its 18s small-subunit ribosomal dna (ssu rdna) and 600 bp of its chitin synthase-2 gene. our phy ... | 2001 | 11136789 |
| structure elucidation of sphingolipids from the mycopathogen sporothrix schenckii: identification of novel glycosylinositol phosphorylceramides with core manalpha1-->6ins linkage. | acidic glycosphingolipid components were extracted from the mycelium form of the thermally dimorphic mycopathogen sporothrix schenckii. two fractions from the mycelium form (ss-m1 and ss-m2), having the highest rf values on hptlc analysis, were isolated and their structures elucidated by 1- and 2-d 13c- and 1h-nuclear magnetic resonance spectroscopy, and electrospray ionization mass spectrometry with lithium adduction of molecular ions. the structures of ss-m1 and ss-m2 were determined to be man ... | 2001 | 11162471 |
| detection of gp43 and its1-5.8s-its2 ribosomal rna genes of paracoccidioides brasiliensis in paraffin-embedded tissue. | paracoccidioidomycosis (pcm) is a deep mycosis caused by the thermo-dependent dimorphic fungus paracoccidioides brasiliensis and is prevalent in latin american countries. an increase in pcm has been reported in recent years and the disease is now recognized as one of the imported fungal infections in japan. to date, more than 15 cases of pcm have been reported in our country, and five of them were diagnosed by clinical and histopathological findings without mycological study. we applied 2 nested ... | 2001 | 11173332 |
| paracoccidioides brasiliensis isolated from armadillos is virulent to syrian hamsters. | isolates of paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. the present study compared the morphology and pathogenicity to hamsters of two p. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (pb-18) and the other isolate recovered from hamsters inoculated with organ homogenates from armadillos (pb-t). the microscopic morphology of pb-18 and pb-t showed yeast cells with similar diameter. how ... | 1999 | 11189763 |
| pathogenesis ii: fungal responses to host responses: interaction of host cells with fungi. | most of our knowledge concerning the virulence determinants of pathogenic fungi comes from the infected host, mainly from animal models and more recently from in vitro studies with cell cultures. the fungi usually present intra- and/or extracellular host-parasite interfaces, with the parasitism phenomenon dependent on complementary surface molecules. among living organisms, this has been characterized as a cohabitation event, where the fungus is able to recognize specific host tissues acting as ... | 2000 | 11204137 |
| molecular typing of pathogenic fungi. | in this round table, the application of several methods of molecular typing were discussed in reference to four important pathogenic fungi: coccidioides immitis, histoplasma capsulatum, candida albicans and paracoccidioides brasiliensis. among the different methods the following were discussed: restriction fragment length polymorphisms (rflp), single nucleotide polymorphisms, random amplified polymorphic dna (rapd), polymerase chain reaction (pcr)-rflp and microsatellites. by means of these meth ... | 2000 | 11204145 |
| mycoses associated with aids in the third world. | despite advances in diagnosis and treatment, the epidemiological status of the human immunodeficiency virus (hiv) infection is far from under control in most of the developing world. sub-saharan africa, southeast asia and india show increased rates of new infections. in latin america and the caribbean there were 1.6 million estimated cases of hiv-infected patients at the end of 1997. fungal diseases have been one of the most relevant diagnoses in relation to the acquired immunodeficiency syndrom ... | 2000 | 11204155 |
| fungal morphogenesis and virulence. | phenotypic variability in pathogenic fungi has long been correlated with virulence, but specific genetic and molecular mechanisms are only recently being unraveled. fungal morphogenesis, reflecting the expression of several regulated genes, and the capacity of the rising forms or phases to cause disease has been focused on at the xivth congress of the international society for human and animal mycology. three experimental models of pathogenic fungi have been discussed. in cryptococcus neoformans ... | 2000 | 11204167 |
| pathogenesis i: interactions of host cells and fungi. | the interactions of host cells and fungi during infection represent a complex interplay. although t helper 1 (th1)-mediated immunity is primarily responsible for acquired resistance to paracoccidioides brasiliensis, studies have demonstrated that polymorphonuclear neutrophils play a critical role in providing an early resistance to this organism. one study has shown that the invasiveness of candida albicans requires adherence, particularly to endothelial cells, which in turn are stimulated to ex ... | 2000 | 11204170 |
| in vitro susceptibilities of paracoccidioides brasiliensis yeast form to antifungal drugs. | a total of 12 paracoccidioides brasiliensis yeast form strains, including clinical and environmental isolates, were tested for their susceptibilities to amphotericin b, ketoconazole, fluconazole, and itraconazole. the tests were determined using a broth macrodilution procedure. in general, there was a remarkable homogeneity of results for all strains, and comparable mics were found for environmental and clinical isolates. | 2000 | 11204357 |
| rflp analysis reveals marked geographical isolation between strains of paracoccidioides brasiliensis. | restriction fragment length polymorphism (rflp) was performed on 32 isolates of the pathogenic fungus paracoccidioides brasiliensis from geographically separated regions of south america. the use of hinfi and hincii gave clear rflp patterns, for which high discriminatory indices could be calculated. computational analysis of the rflp patterns for the 32 isolates suggested that at least five groups of strains existed, each of which was geographically distinct and corresponded closely with present ... | 2000 | 11204881 |
| antibody isotypes to a paracoccidioides brasiliensis somatic antigen in sub-acute and chronic form paracoccidioidomycosis. | this report describes the differences in isotype antibody reactivity against a crude paracoccidioides brasiliensis antigenic preparation in the sub-acute (saf) and chronic (cf) forms of paracoccidioidomycosis before treatment. igg antibodies were detected in all patients, with a slightly but not significantly higher reactivity in the saf. igg1 antibodies were present, frequently at high levels, in both forms, whereas igg3 was always low or absent. igg2 antibodies were detectable in most patients ... | 2001 | 11211219 |
| paracoccidioides brasiliensis in a postpartum pap smear. a case report. | there are several reported cases that describe female genital tract infections with opportunistic fungi, such as blastomyces dermatitidis, coccidioides immitis, aspergillus flavus, cryptococcus neoformans and mucor. we describe a case of paracoccidiodomycosis limited to the uterine cervix. to the best of our knowledge, no such case has been described before in the english-language literature. | 2001 | 11213509 |
| imbalance of il-2, ifn-gamma and il-10 secretion in the immunosuppression associated with human paracoccidioidomycosis. | patients with paracoccidioidomycosis (pcm) display a certain degree of immunecompromise characterized by lymphocyte hyporesponsiveness to the main paracoccidioides brasiliensis antigen (gp43). to determine whether cytokines are involved in this state, we evaluated the secretion of il-2, il-10 and ifn-gamma by peripheral blood mononuclear cells (pbmc) from patients with the acute (af) and chronic (cf) forms of pcm and from healthy, p. brasiliensis-sensitized subjects. gp43-stimulated pbmc from he ... | 2001 | 11237434 |
| the low efficiency of dendritic cells and macrophages from mice susceptible to paracoccidioides brasiliensis in inducing a th1 response. | in the present study we evaluated t cell proliferation and th lymphokine patterns in response to gp43 from paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. t cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. the pattern of t cell lymphokines stimulated by dendritic cells was always th1, although the levels of il-2 and ifn-gamma were lower in t cell cultures from s ... | 2001 | 11285466 |
| characterization of monoclonal antibody mest-2 specific to glucosylceramide of fungi and plants. | an igg2a monoclonal antibody anti-glucosylceramide was established and termed mest-2. high performance thin layer chromatography immunostaining, and solid-phase radioimmunoassay showed that mest-2 reacts with glucosylceramide from yeast and mycelium forms of paracoccidioides brasiliensis, histoplasma capsulatum, and sporothrix schenckii; from hyphae of aspergillus fumigatus; and from yeast forms of candida albicans, cryptococcus neoformans, cryptococcus laurentii, and cryptococcus albidus. studi ... | 2001 | 11287397 |
| modulatory effect of prostaglandins on human monocyte activation for killing of high- and low-virulence strains of paracoccidioides brasiliensis. | the effect of indomethacin (indo), a cyclo-oxygenase inhibitor, on the monocyte-mediated killing of a low- (pb265) and a high- (pb18) virulence strain of paracoccidioides brasiliensis was examined. the pb18 strain was not killed by either non-activated or interferon-gamma (ifn-gamma) -activated human monocytes but these cells did show fungicidal activity if pretreated with indo. in contrast with ifn-gamma, tumour necrosis factor-alpha (tnf-alpha) was very effective at stimulating the fungicidal ... | 2001 | 11328382 |
| paracoccidioidomycosis in colombia: an ecological study. | the natural habitat of paracoccidioides brasiliensis, agent of paracoccidioidomycosis (pcm), remains unknown. this study is aimed at establishing associations between the ecological variables present in all colombian municipalities and the incidence of pcm. records of 940 patients were studied and several ecological variables analysed, as well as their association to amount of patients per total rural population in each municipality, determined through a multivariate analysis. all 940 patients c ... | 2001 | 11349982 |
| oral exfoliative cytology in the diagnosis of paracoccidioidomycosis. | to assess the efficacy of conventional oral exfoliative cytology as a diagnostic tool in paracoccidioidomycosis. | 2001 | 11393067 |
| cerebriform colonies of paracoccidioides brasiliensis isolated from nine-banded armadillos (dasypus novemcinctus) at room temperature. | twelve isolates of paracoccidioides brasiliensis generated cerebriform colonies at room temperature on potato glucose agar slants (pda). these isolates contained abundant chlamydospores and yeast-like cells and are a subset of the 65 isolates obtained from nine-banded armadillos (dasypus novemcinctus). they grew as a yeast form with typical multiple buddings at 37 degrees c on brain heart infusion agar supplemented with 1% glucose. after replating on pda and culturing at room temperature for 2 m ... | 2001 | 11398643 |
| two-dimensional electrophoresis and characterization of antigens from paracoccidioides brasiliensis. | paracoccidioides brasiliensis is a fungal pathogen of humans. to identify antigens from p. brasiliensis we fractionated a crude preparation of proteins from the fungus and detected the igg reactive proteins by immunoblot assays of yeast cellular extracts with sera of patients with paracoccidioidomycosis (pcm). we identified and characterized six new antigens by amino acid sequencing and homology search analyses with other proteins deposited in a database. the newly characterized antigens were hi ... | 2001 | 11418327 |
| the habitat of paracoccidioides brasiliensis: how far from solving the riddle? | when trying to understand the pathophysiology of any infectious agent, one key piece of information is the determination of its habitat. in the case of paracoccidioides brasiliensis, the precise location of the fungus' environmental niche remains undefined despite the efforts of various research groups. this review summarizes recent studies on the ecology of p. brasiliensis and certain facets of paracoccidioidomycosis. studies on the juvenile form of paracoccidioidomycosis in children less than ... | 2001 | 11446526 |