Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [isolation of an paracoccidioides brasiliensis exoantigen from solid culture media]. | the goal of this work was to develop in solid medium a fast method to obtain paracoccidioides brasiliensis (pb) with a high yield. four culture media were assayed: sabouraud honey-agar, sabouraud dextrose-agar, tomato -agar-medium (tom) and a medium based on grape pulp. the most exhuberant growth was observed in medium based on grape pulp. antigen was prepared in microscale at 6, 10 and 15 days incubation of solid cultures and the crude product concentrated by means of centriplus tubes (helena, ... | 1995 | 8588052 |
| paracoccidioidomycosis and aids: an overview. | the scarcity of reported cases of paracoccidioidomycosis and aids remains unexplained. we review the details of the 27 cases reported in the medical literature. paracoccidioidomycosis occurs in patients with advanced aids who are not receiving prophylaxis for pneumocystis carinii pneumonia with trimethoprim-sulfamethoxazole, which is also effective against paracoccidioides brasiliensis. clinical manifestations include prolonged fever, weight loss, generalized lymphadenopathy, splenomegaly, hepat ... | 1995 | 8589154 |
| the hamster cheek pouch: an immunologically privileged site suitable to the study of granulomatous infections. | the hamster cheek pouch is an invagination of oral mucosa, characterized histologically as skin-like. in this paper we describe anatomical, histological and embriological features of the pouch and comment on the pouch as an immunologically privileged site since it lacks lymphatic drainage and has few langerhans cells. we present the review from literature and our observations after inoculation in the pouch of mycobacteriae (bcg, mycobacterium tuberculosis and mycobacterium leprae) and a fungus ( ... | 1995 | 8599058 |
| neutrophil oxidative metabolism and killing of p. brasiliensis after air pouch infection of susceptible and resistant mice. | the oxidative burst of polymorphonuclear neutrophils (pmn) and their ability to inhibit paracoccidioides brasiliensis growth was studied in susceptible (b10.a) and resistant (a/j) mice. the cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure pmn preparations; the number of cells was similar for both strains at 24 h and five times higher in the resistant strain at 15 days. the oxidative metabolism of these pmn was evaluated by the luminol and lucigen-enhanced c ... | 1996 | 8613700 |
| cloning, characterization, and epitope expression of the major diagnostic antigen of paracoccidioides brasiliensis. | the 43,000-da glycoprotein (gp43) of paracoccidioides brasiliensis is an immunodominant antigen for antibody-dependent and immune cellular responses in patients with paracoccidioidomycosis. in order to identify the peptide epitopes involved in the immunological reactivities of the gp43 and to obtain highly specific recombinant molecules for diagnosis of the infection, genomic and cdna clones representing the entire coding region of the antigen were sequenced. the gp43 open reading frame was foun ... | 1996 | 8626811 |
| antigen-specific immunosuppression in paracoccidioidomycosis. | to characterize the immune dysfunction associated with paracoccidioidomycosis, we studied the in vitro lymphocyte reactivity to phytohemagglutinin (pha), pokeweed mitogen (pwm), a candida albicans antigen (cma), and a paracoccidioides brasiliensis antigen (pbag) in 32 patients with the acute and the chronic form of the disease before or during the initial phase of treatment and after clinical cure. we also studied, as controls, 30 healthy individuals, 15 of them immune to p. brasiliensis. result ... | 1996 | 8651374 |
| ornithine decarboxylase in paracoccidioides brasiliensis. | ornithine decarboxylase in paracoccidioides brasiliensis, a dimorphic human pathogenic fungus, was more active at 37 degrees c in the yeast phase and at 30 degrees c in the mycelial phase. in contrast to other fungal systems, yeast growth and mycelium-to-yeast transition in p. brasiliensis were accompanied by a high activity of ornithine decarboxylase at the onset of the budding process, the activity of which was inhibited by 1,4-diamino-2-butanone. the activity of ornithine decarboxylase remain ... | 1996 | 8661922 |
| structural characterization of a new galactofuranose-containing glycolipid antigen of paracoccidioides brasiliensis. | an acidic glycolipid (band 1), purified from p. brasiliensis by a combination of ion exchange chromatography, hplc, and hptlc, was found to be reactive with sera of all patients with paracoccidioidomycosis (pcm). monosaccharide analysis of band 1 yielded mannose and galactose in a 2:1 ratio, while mild acid hydrolysis and mild periodate oxidation/nab3h4 reduction indicated the presence of a terminal galactofuranose. preliminary analysis of 1h-nmr and ms data suggests that the structure of the gl ... | 1996 | 8670257 |
| immunochemical study of a paracoccidioides brasiliensis polysaccharide-like antigen. | the polysaccharide antigen from p. brasiliensis has been largely employed in serologic tests ,as well as in skin tests, to evaluate cellular immunity. sds-page analysis of this antigen has revealed a variability in the number of bands exhibited by isolates sn, 265, 339, 113, and 18 (7 to 16 bands). the antigens obtained from isolates 2, ptl, 192 and adel showed two or three bands. glycoprotein analysis demonstrated a broad region between 50 and 90 kda. major bands of 48 and 30 kda were present i ... | 1995 | 8683406 |
| paracoccidioidomycosis in a renal transplant recipient. | paracoccidioidomycosis is a fungal infection rarely described in immunodeficient patients. we report a severe case of pulmonary paracoccidioidomycosis in a renal transplant recipient and demonstrate deficiencies of in vitro lymphocytic transformation assays, skin hypersensitivity tests, as well as low levels of antibodies to paracoccidioides brasiliensis. | 1995 | 8683410 |
| an atypical isolate of paracoccidioides brasiliensis. | an atypical strain of paracoccidioides brasiliensis was obtained from a patient with disseminated paracoccidioidomycosis. this isolate, which has been maintained in the laboratory for 4 years by successive subcultures (30-40 days) in fava netto medium, produces cerebriform colonies characteristic of the yeast phase of the fungus when left at room temperature. microscopy shows a predominance of yeasts with multiple buds, side by side with some branching filamentous structures presenting intercala ... | 1995 | 8720200 |
| cellular immunity and immunostimulation in paracoccidioidomycosis. | we have evaluated clinical and laboratorial effects of immunostimulation scarification with antigen of paracoccidioides brasiliensis in patients with paracoccidioidomycosis, who often show depressed immunity. we have studied 62 brazilian patients with paracoccidioidomycosis, 46 with active disease and 16 cured, in a 10-year period between 1982 and 1992. among the 46 patients with active disease: 10 had acute form, 12 chronic unifocal form and 24 the chronic multifocal form of the disease; 16 sho ... | 1996 | 8766739 |
| evaluation and comparison of the growth and viability of yeast-like cells of paracoccidioides brasiliensis. | the growth and viability of two paracoccidioides brasiliensis strains were evaluated in pyg and mmcm media containing no boric acid. the two p. brasiliensis strains exhibited a similar growth pattern in pyg medium, which was considered to be good compared with the growth curves in defined mmcm medium without boric acid. calculation of cell generation time in the media studied demonstrated that the cells had greater difficulty in forming new generations in mmcm medium containing no boric acid. th ... | 1996 | 8767000 |
| histopathology of paracoccidioidomycotic infection in athymic and euthymic mice: a sequential study. | we report the comparative and sequential histologic analysis of lesions developed by paracoccidioides brasiliensis-infected athymic and euthymic balb/c mice, using conventional and specific stain to characterize the morphology of fungi and inflammatory cells as well as the extracellular matrix patterns. in both groups, neutrophils and macrophages were the predominant cells; macrophage transition to giant and epithelioid cells was observed. degenerated fungi and fungal dust were associated with l ... | 1996 | 8780467 |
| paracoccidioides brasiliensis-gp43 used as paracoccidioidin. | a purified glycoprotein of 43,000 daltons from paracoccidioides brasiliensis (gp43) was tested as paracoccidioidin in delayed-type hypersensitivity (dth) tests in both experimental animals (guinea pig and mice) and patients with paracoccidioidomycosis (pcm). the gp43 paracoccidioidin was compared with the traditional fava netto antigen (agfn). in guinea pigs, the intradermal injection of 2 micrograms of gp43 showed a similar response to those obtained with agfn, showing in histological sections ... | 1996 | 8803794 |
| immunochemical and subcellular localization of the 43 kda glycoprotein antigen of paracoccidioides brasiliensis with monoclonal antibodies. | two monoclonal antibodies st-7 (igg1) and st-8 (igg2b), directed against a 43 kda glycoprotein (gp43) of paracoccidioides brasiliensis were produced. it was possible to detect the gp43 by elisa in amounts as low as 100 ng per well, and by western blot about 300 ng were detected. mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with st-7 and st-8, which suggests that these mabs recognize peptide epitopes. confirming the periodate oxidation data, the 38 kda protein ... | 1996 | 8803798 |
| molecular cloning, nucleotide sequencing, and characterization of a 27-kda antigenic protein from paracoccidioides brasiliensis. | a gene encoding a 27-kda antigenic protein from paracoccidioides brasiliensis was cloned, sequenced, and characterized. a cdna library of the mycelial phase was produced and packed in uni-zap-xr vector, lambda zap ii synthesis kit (stratagene, la jolla, ca). the screening of the library was carried out using a pool of sera from paracoccidioidomycosis patients that had proven reactive in serological testing. among 44,000 immuno-screened clones from the library, 2 were positive (clones 2 and 3). t ... | 1996 | 8810517 |
| inoculation experimental animals with paracoccidioides brasiliensis strains: an attempt to reestablish the dimorphic process and variation in pathogenicity as a function of time of preservation under mineral oil. | in an attempt to reestablish the dimorphic process in strains of paracoccidioides brasiliensis in the transition phase (y reversible m) and to reisolate them, five strains in the transitional phase due to the long time of preservation under mineral oil and two strains in the yeast-like phase were inoculated into male albino rats. the animals were then studied for the presence of paracoccidioidomycotic granulomata. of the seven strains inoculated, five caused granulomatous nodules in several orga ... | 1996 | 8817931 |
| cutaneous manifestations of systemic mycoses. | systemic fungal diseases are primary pulmonary diseases caused by the dimorphic fungal pathogens, blastomyces dermatitides, coccidioides immitis. histoplasma capsulatum, or paracoccidioides brasiliensis. infection occurs after inhalation of the infectious form of the fungus and may be acute, self-limited, or subclinical. primary cutaneous infection occurs only after traumatic implantation of the fungus and is unusual. erythema nodosum or erythema multiforme may accompany the acute form of the di ... | 1996 | 8821165 |
| pulmonary paracoccidioidomycosis misdiagnosed as pneumocystis pneumonia in an immunocompromised host. | yeast cells of paracoccidioides brasiliensis can resemble the cysts of pneumocystis carinii in smears stained with grocott's modification of the gomori methanamine silver stain. furthermore, p. brasiliensis can cross-react in material stained with a widely used p. carinii immunofluorescent stain which uses monoclonal antibodies. the need to differentiate p. brasiliensis and p. carinii will become more important as the increasing incidence of immunosuppression results in the reactivation of laten ... | 1996 | 8862614 |
| cycloactive, aneugenic and clastogenic effects of paracoccidioides brasiliensis exoantigen in human lymphocyte cultures. | the in vitro effect of paracoccidioides brasiliensis exoantigen on the human lymphocytes cell cycle and chromosomes was studied. human peripheral blood lymphocyte cultures from ten healthy, white, non-smoking, non-related adult males (mean age 31.3 +/- 8.2 years) were studied. blood cultures were treated with three exoantigen concentrations (0.25, 2.50 and 10.00 micrograms ml-1). at least 1000 metaphases were analysed at each concentration, for evaluation of numerical and structural chromosome a ... | 1996 | 8873883 |
| evaluation of paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis. | the exoantigen of paracoccidioides brasiliensis standardized by camargo et al. (agr) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (pbm). fava netto antigen (agf) was tested in parallel as a control antigen. the study was conducted with mice and guinea pigs infected with p. brasiliensis or immunized with its fungal antigens, on patients with pbm and on their respective control groups. the cell immune respons ... | 1996 | 8873886 |
| structure of the n-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus paracoccidioides brasiliensis. | the major diagnostic antigen of paracoccidioides brasiliensis is the exocellularly secreted 43,000 da glycoprotein (gp43) which contains a single n-linked oligosaccharide chain. this oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence. it contains a neutral high-mannose core (man7glcnac2) to which a (1-->6)-linked alpha-d-manp chain of variable length, substit ... | 1996 | 8877371 |
| paracoccidioides brasiliensis infection in nude mice: studies with isolates differing in virulence and definition of their t cell-dependent and t cell-independent components. | athymic and euthymic balb/c mice infected with highly (pb18) or slightly (pb265) virulent paracoccidioides brasiliensis isolates were compared regarding mortality, presence of viable yeasts, specific immunoglobulin m (igm) and igg titers, and the antigen recognition patterns of these antibodies. isolate pb18 caused a more severe disease in athymic mice, as supported by higher number of infected organs and shorter survival times. these animals, however, were resistant to pb265 infection. high tit ... | 1996 | 8916794 |
| the use of glucan as immunostimulant in the treatment of paracoccidioidomycosis. | a group of 10 patients, nine of them seriously infected with paracoccidioides brasiliensis (g1), received glucan (beta-1,3 polyglucose) as an immunostimulant intravenously once a week for one month, followed by monthly doses (10 mg) over an 11-month period, together with a specific anti-fungal agent as an immunostimulant. a second group of eight moderately infected patients (g2) was treated with only the anti-fungal agent. among the patients in g1, there was only one case of relapse compared wit ... | 1996 | 8940980 |
| monoclonal antibodies against the 43,000 da glycoprotein from paracoccidioides brasiliensis modulate laminin-mediated fungal adhesion to epithelial cells and pathogenesis. | the surface glycoprotein gp43, a highly immunogenic component of paracoccidioides brasiliensis, is used in the serodiagnosis of paracoccidioidomycosis (pcm) and has recently been shown to specifically bind the extracellular matrix protein laminin. binding to laminin induces the increased adhesion of the fungus to epithelial cells; a hamster testicle infection model has shown that the gp43-dependent binding of fungal cells to laminin enhances their pathogenicity in vivo. we report on the producti ... | 1996 | 8985752 |
| osteomyelitis by paracoccidioides brasiliensis (south american blastomycosis): cytologic diagnosis on fine-needle aspiration biopsy smears: a case report. | a case of osteomyelitis caused by paracoccidioides brasiliensis primarily diagnosed by means of fine-needle aspiration biopsy is reported here in a 60-yr-old italian patient who had lived in venezuela for 40 yr. the cytologic and electron microscopic features of the exudate aspirated from a left femoral osteolytic area are described, and the differential diagnosis of this mycotic infection is discussed briefly. | 1996 | 8989552 |
| immunological characterization of a recombinant 27-kilodalton antigenic protein from paracoccidioides brasiliensis. | we report the expression in escherichia coli of a 27-kda antigenic protein from paracoccidioides brasiliensis. when analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. no cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis. | 1996 | 8991645 |
| paracoccidioides brasiliensis antigen batches from the same isolate show immunological and biochemical differences. | we investigated the occurrence of antigenic and biochemical variability among paracoccidioides brasiliensis antigen batches prepared according to the same protocol. initially (experiment #1), we analyzed two antigen lots of two human isolates (bt1 & bt2), cultured in two media (pyg: bactopeptone, yeast extract, glucose; mmm: mcveigh & morton medium) in sds-page and in two immunological tests (immunodiffusion-id and footpad swelling test-fpt). afterwards (experiment #2), we compared the antigenic ... | 1996 | 9008879 |
| use of morphology index histograms to quantify populations of the fungal pathogen paracoccidioides brasiliensis. | to quantify the dimorphic process in wild and mutant strains of paracoccidioides brasiliensis, we defined a morphology index (mi) in terms of the maximum cell length (l), maximum cell diameter (d), and septal diameter (s), according to the equation mi = 2.13 + 1.13 log10 (ls/d2), whose intercept and slope were such that mi was around 1 for yeast (spherical) cells or 4 for hyphal (elongated) cells. this discriminatory power was used to quantify morphological population mixtures through mi histogr ... | 1997 | 9025294 |
| differences in reactivity of paracoccidioidomycosis sera with gp43 isoforms. | the glycoprotein gp43 from paracoccidioides brasiliensis is the main antigenic component in paracoccidioidomycosis (pcm) because it is recognized by 100% of pcm patients. it has also been shown that different fungal strains produce gp43 with at least four isoform profiles. in this study, different isoform profiles from gp43, with pis ranging from 5.8 to 8.5, were affinity purified from various p. brasiliensis (b-339, s.s., 1925 and i9) exoantigens. because of the isoform heterogeneity, we questi ... | 1997 | 9061580 |
| laminin-binding epitope on gp43 from paracoccidioides brasiliensis is recognized by a monoclonal antibody raised against staphylococcus aureus laminin receptor. | adhesion is regarded as an important step in the pathogenesis of several microorganisms. thus, the ability to recognize extracellular matrix proteins, such as laminin or fibronectin, has been correlated with invasiveness. studying the already characterized laminin-binding protein of paracoccidioides brasiliensis, the 43 kda glycoprotein (gp43), we evaluated whether mab 1.h12, raised against the laminin-binding protein from staphylococcus aureus, cross-reacts with that fungal protein. by immunobl ... | 1997 | 9061584 |
| epidemiologic skin test survey of sensitivity to paracoccidioidin, histoplasmin and sporotrichin among gold mine workers of morro velho mining, brazil. | skin tests with paracoccidioidin, histoplasmin and sporotrichin were applied to 417 workers of morro velho mining in the state of minas gerais, brazil, with the main purpose of detecting the prevalence of paracoccidioidomycosis-infection, histoplasmosis capsulate-infection and sporotrichosis-infection. the rates of positivity to the skin tests were 13.43% for paracoccidioidin, 17.50% for histoplasmin and 13.67% for sporotrichin. several epidemiological factors were investigated for a better inte ... | 1996 | 9063004 |
| polyamines in growth and dimorphism of paracoccidioides brasiliensis. | putrescine and spermidine were the only polyamines found in paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans. free polyamines (putrescine > spermidine) increased during the first 24 h of yeast growth, with a second peak at 42 h, and also during the first 12 h of mycelium-to-yeast transition (spermidine > putrescine). conjugated and bound polyamines were also quantified. 1, 4-diamino-2-butanone decreased free putrescine and spermidine accumulation by inhibiting the activity ... | 1996 | 9082919 |
| in vitro amphotericin b effects on growth, viability and dimorphism of paracoccidioides brasiliensis: reversal of the treatment. | the in vitro effects of amphotericin b deoxycholate suspension (fungizone) on paracoccidioides brasiliensis growth, cell viability and transformation were investigated. we also analyzed the protein synthesis patterns of both cellular forms, yeast and mycelium in the presence of amb. this drug, at 30 micrograms/ml, highly inhibited yeast growth, which could be recovered depending on treatment time, where the most effective reversion was observed after 6 hr of incubation. the yeast cell viability, ... | 1997 | 9087951 |
| intramedullary spinal cord paracoccidioidomycosis. report of two cases. | two cases of intramedullary paracoccidioidomycosis are reported. paracoccidioidomycosis is a systemic disease that involves the buccopharyngeal mucosa, lungs lymph nodes and viscera and infrequently the central nervous system. localization in the spinal cord is rare. case 1: a 55-year old male admitted with crural pararesis, tactile/painful hypesthesia and sphincter disturbances of 15 days duration. cutaneous-pulmonary blastomycosis was diagnosed 17 years ago. myelotomography showed a blockade o ... | 1996 | 9109993 |
| thoracic intraspinal paracoccidioidomycosis. case report. | intramedullary lesions caused by paracoccidioides brasiliensis have been rarely described. its diagnosis may be challenging and surgical approach is indicated for diagnostic and therapeutic purposes. we hereby report a case with mri and surgical findings in a 45 year-old woman with intramedullary paracoccidioidomycosis, and make a review of other cases presented in the literature. | 1996 | 9109994 |
| immunosuppression in paracoccidioidomycosis: t cell hyporesponsiveness to two paracoccidioides brasiliensis glycoproteins that elicit strong humoral immune response. | to assess human cellular immune response to paracoccidioidomycosis (pcm), lymphocyte proliferative responses to purified antigens from paracoccidioides brasiliensis were determined in healthy persons previously infected by the fungus (positive donors), in healthy noninfected persons (controls), and in pcm patients. affinity-purified gp70 and gp43, the two major antigens in humoral immune responses, were used. both induced lymphocyte proliferation (gp43 species-specific) in positive donors but no ... | 1997 | 9129101 |
| human chromosome aberrations induced in vitro by paracoccidioides brasiliensis glycoproteic component (gp 43). | the in vitro cytogenetic effects of the 43-kda molecular mass exocellular glycoproteic component (gp 43) from paracoccidioides brasiliensis were studied in cultures from human lymphocytes. the sample included 10 healthy, white, non-smoking, non-related males (mean age of 31.3 +/- 8.2 years). besides the control, three concentrations of gp 43 (0.125, 1.25 and 5 micrograms/ml) were used. in each group, around 1000 cells were examined in search of chromosome aberrations, and 30000 metaphases were a ... | 1997 | 9150747 |
| inhibition of paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis. | in paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. the species observed were phosphatidylcholine (pc, 30-40%), phosphatidylethanolamine (pe, 27-28%), phosphatidylserine (16-19%), phosphatidylinositol (13-17%) and sphingomyelin (3-5%). the main fatty acids found in the yeast (y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61%) ... | 1997 | 9168609 |
| a monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from paracoccidioides brasiliensis: cross-reactivity with leishmania major and trypanosoma cruzi. | a mouse monoclonal antibody, mest-1, was produced against band 1 glycolipid antigen of paracoccidioides brasiliensis. the glycan structure of band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: galf beta 1-->6(manp alpha 1-->3)manp beta 1-->2ins. the reactivity of mest-1 mab was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. selective oxidation of galactofuranose residues and inhibition assays with dif ... | 1997 | 9184826 |
| cross-reactivity in histoplasma capsulatum variety capsulatum antigen assays of urine samples from patients with endemic mycoses. | we evaluated cross-reactivity in the antigen assay used for the diagnosis of histoplasmosis by testing urine samples from patients with disseminated fungal infections. the mycoses chosen for this study were selected on the basis of the observation that during clinical testing, cross-reactions may occur between histoplasma capsulatum var. capsulatum, paracoccidioides brasiliensis, blastomyces dermatitidis, coccidioides immitis, and penicillium marneffei. we detected antigen in 12 of 19 patients w ... | 1997 | 9195077 |
| molecular detection and identification of paracoccidioides brasiliensis. | nearly 800 nucleotides from the 5' terminus of the 28s ribosomal gene of paracoccidioides brasiliensis were sequenced, and a 14-base dna probe specific for this species was identified. hybridization results showed that the probe identified p. brasiliensis ribosomal dna in a panel of ribosomal dnas representing a total of 48 species of fungi. | 1997 | 9196219 |
| isolation and possible composition of glucosylceramides from paracoccidioides brasiliensis. | twelve different species of neutral monohexosyl ceramides (cmhs) and two species of neutral monohexosyl ceramides were isolated from mycelium and yeast forms of paracoccidioides brasiliensis, respectively, by a combination of ion-exchange chromatography, hplc, and hptlc. the glucosylceramides did not react with sera from patients with paracoccidioidomycosis (pcm). carbohydrate analysis indicated that cmhs contain glucose. analysis of 1h-nmr and mass spectrometry data suggest that the structure o ... | 1996 | 9196542 |
| the asteroid body of lobomycosis. | lobomycosis is a dermal mycosis produced by loboa loboi. it gives rise to dermal granulomas in which giant cells phagocytose an overwhelming number of fungi. intracytoplasmatic asteroid bodies (ab) have been observed in some giant cells. their nature is unknown and they have been confused with the sporotrichotic ab. we studied 84 skin biopsies from 53 patients with lobomycosis, 7 by electron microscopy (em). immunohistochemistry with a polyclonal anti-sporothrix schenckii antibody was performed ... | 1996 | 9208474 |
| electrophoretic karyotype of clinical isolates of paracoccidioides brasiliensis. | chromosomal dna molecules were obtained from eight clinical isolates of paracoccidioides brasiliensis during the early staged of the yeast to mycelial transformation process and were separated by contour-clamped homogeneous electric field gel electrophoresis. in seven of the isolates, it was possible to separate five bands, albeit with two different migration patterns (a and b). in the remaining isolate only four bands were observed (pattern c). the isolated chromosomes showed molecular sizes ra ... | 1997 | 9228790 |
| protein synthesis patterns of paracoccidiodes brasiliensis isolates in stage-specific forms and during cellular differentiation. | in this paper we compared the protein synthesis patterns of paracoccidioides brasiliensis isolates. the protein profiles were compared for both yeast and mycelial forms and similarity analysis among them was performed by calculating similarity matrices and grouping the isolates in dendrograms. the examined isolates exhibited highly variable cellular morphology at 36 degrees c, when typical yeast cells were expected. on the other hand, at 26 degrees c all the isolates showed mycelial morphology. ... | 1997 | 9229337 |
| igg, igm and iga antibody response for the diagnosis and follow-up of paracoccidioidomycosis: comparison of counterimmunoelectrophoresis and complement fixation. | igg, igm and iga antibodies to gp43 (glycoprotein fraction of paracoccidioides brasiliensis) were measured by elisa in 63 samples from 23 patients with paracoccidioidomycosis before and twice after chemotherapy was started. antibodies against p. brasiliensis were detected by indirect immunofluorescence (if) (igg, igm and iga isotypes), counterimmunoelectrophoresis (cie) and complement fixation. two control groups composed of 19 healthy individuals and 12 patients with other diseases (six with hi ... | 1997 | 9229338 |
| cellular requirements for immunomodulatory effects caused by cell wall components of paracoccidioides brasiliensis on antibody production. | in a previous study, we reported an increase in the number of immunoglobulin-secreting cells and the augmentation of antibody production (igm and igg3) against unrelated antigens (sheep erythrocytes or bovine serum albumin (bsa)) in mice infected with the fungus paracoccidioides brasiliensis as well as in mice inoculated with its cell wall preparation (cw). the immunomodulatory effect of the live fungus and cw preparation was dose-dependent and mainly restricted to the i.p. inoculation simultane ... | 1997 | 9276521 |
| isolation of fungi from nature in the region of botucatu, state of são paulo, brazil, an endemic area of paracoccidioidomycosis. | in an attempt to isolate paracoccidioides brasiliensis from nature 887 samples of soil from botucatu, sp, brazil, were collected cultured in brain heart infusion agar supplemented with dextrose, in potato dextrose agar and in yeast extract starch dextrose agar, all with antibiotics, at 25 degrees and 37 degrees c. five thermo-dependent dimorphic fungi morphologically resembling p. brasiliensis were isolated; two from armadillo holes; further studies of the biology, antigenicity and genetic featu ... | 1996 | 9283642 |
| changes in plasmatic biochemical parameters during experimental murine paracoccidioidomycosis. | determinations of plasma cholesterol, hdl-cholesterol, triglycerides, total proteins, bilirubin, aspartate transaminases (ast), glucose, iron and total iron binding capacity were evaluated after 15 and 90 days of intraperitoneal infection with 5 x 10(6) virulent yeasts of paracoccidioides brasiliensis pb 18 isolate in resistant (a/j) and susceptible (b10.a) mice. significant differences between control and infected groups were seen: an increase of triglycerides and total proteins for both strain ... | 1997 | 9292419 |
| model of in vitro granulomatous hypersensitivity in human paracoccidioidomycosis. | with the purpose of studying the immunological components of granulomatous hypersensitivity in patients infected with paracoccidioides brasiliensis, we used the model of in vitro granuloma formation developed for schistosomiasis studies, that correlates with in vivo granulomatous reactivity occurring around eggs trapped in organs of infected donors. in this case, granuloma formation can be determined examining cellular reactivity manifested as multiple cell layers surrounding antigen-conjugated ... | 1997 | 9368406 |
| localization of glycoconjugates and glycogen in yeast-like cells and protoplasts of paracoccidioides brasiliensis. | the presence and localization of storage polysaccharides and of polysaccharides as cell structure constituents of paracoccidioides brasiliensis yeast-like cells and protoplasts were studied using the periodic acid-thiocarbohydrazide-silver proteinate (patag) technique. yeast-like cells presented glycogen particles in the form of rosettes, which were mostly concentrated in regions of the cytoplasm. cells in the budding process presented small amounts of glycogen in their matrix. the intracellular ... | 1997 | 9375493 |
| [monoarthritis of the knee as an isolated manifestation of paracoccidioidomycosis]. | osteoarthritis in paracoccidioidomycosis has been rarely reported. the present case describes a 36-"year"-old woman, with chronic monoarthritis in the knee lasting 4 years. the diagnosis was achieved only after synovial biopsy, by anatomopathological examination showing granulomatous reaction with a large number of the characteristic "pilot wheel"paracoccidioides brasiliensis yeast cells. specific therapy, initially with ketoconazole and followed by cotrimoxazole led to complete functional recov ... | 1997 | 9380899 |
| reactivity of anti-gp43 antibodies from paracoccidioides brasiliensis antiserum with extracts from cutaneous lesions of lobo's disease. preliminary note. | we demonstrated through several immunochemical tests the presence of gp43 from p. brasiliensis in extracts of cutaneous lesions from jorge lobo's disease. this glicoprotein is one of the immunodominant antigens in this species, and is used to identify it. the demonstration of gp43 tissues infected by the agent of jorge lobo's disease is an additional evidence for classifying it in the genera paracoccidioides, species loboi. | 1997 | 9394534 |
| use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens. | the precise diagnosis of paracoccidioidomycosis, in most cases, is established by direct methods and indirect immunological tests. the latter method is reliant on the identification of the host's humoral responses, which are usually impaired or absent in patients with severe juvenile forms of the disease and in immunocompromised patients. determining disease activity or assessing treatment responses by measuring antibody levels is difficult, since antibody titer may remain elevated or persist at ... | 1997 | 9399534 |
| expression of glycoprotein gp43 in stage-specific forms and during dimorphic differentiation of paracoccidioides brasiliensis. | expression of the 43 kda glycoprotein (gp43) was analysed in several paracoccidioides brasiliensis isolates. using one- and two-dimensional analysis of crude cellular extracts, it was shown that protein expression in yeast and mycelium was dependent on the isolate analysed. in two strains, in both yeast and mycelium cells. gp43 was present, whereas expression was restricted to the yeast phase of two other strains. the clinical implications of this phase-specific gp43 expression are uncertain. | 1997 | 9402527 |
| antigenic similarities to paracoccidioides brasiliensis in thermo-dependent dimorphic fungi isolated from soil in botucatu, sp, brazil. | we compared the antigenic characteristics of two thermo-dependent dimorphic fungi isolated from soil in botucatu, an endemic area of paracoccidioidomycosis (pcm) and paracoccidioides brasiliensis. the soil isolates grew as cerebriform colonies at 37 degrees c (yeast form) and as cottonous colonies at 25 degrees c (mycelial form). no pathogenicity for ddy mice or hamsters were observed. in immunodiffusion test, there were precipitation bands between the 2 soil isolates and pooled pcm patient sera ... | 1997 | 9404023 |
| monocyte adherence to paracoccidioides brasiliensis, zymosan-c3b and erythrocyte-hemolysin in patients with paracoccidioidomycosis. | independent and dependent (c3b/fc receptors) opsonic adherence ability of monocytes from thirty-three patients with acute or chronic paracoccidioidomycosis and from 13 healthy individuals were studied in the presence of paracoccidioides brasiliensis (pb), paracoccidioides brasiliensis opsonized by patient's serum (pbps) or normal serum (pbns), zymosan opsonized by fresh sera from healthy donors (zyns) and erythrocytes opsonized by hemolysin (ea). statistically significant differences concerning ... | 1997 | 9433808 |
| mapping of the t-cell epitope in the major 43-kilodalton glycoprotein of paracoccidioides brasiliensis which induces a th-1 response protective against fungal infection in balb/c mice. | the 43-kda glycoprotein of paracoccidioides brasiliensis is the major diagnostic antigen of paracoccidioidomycosis, the prevalent systemic mycosis of latin america. apart from eliciting high antibody titers, gp43 is also immunodominant in delayed-type hypersensitivity reactions in infected animals and humans. the cellular immune response in mice to gp43 administered in complete freund's adjuvant involves cd4+ th-1 lymphocytes, secreting gamma interferon (ifn-gamma) and interleukin 2 (il-2) but n ... | 1998 | 9453642 |
| protective role of gamma interferon in experimental pulmonary paracoccidioidomycosis. | we have developed a murine model of pulmonary infection by paracoccidioides brasiliensis in which resistance was associated with immunological activities governed by gamma interferon (ifn-gamma). to better characterize this model, we measured type 1 and type 2 cytokines in the lungs and investigated the effect of endogenous ifn-gamma depletion by monoclonal antibodies in the course of infection of susceptible (b10.a) and resistant (a/sn) mice. at weeks 4 and 8 after infection, lungs from suscept ... | 1998 | 9453644 |
| paracoccidioides cerebriformis moore, 1935. mycologic and immunochemical study. | the present study concern on mycologic and immunochemical data obtained from two samples of a fungus considered as belonging to the species paracoccidioides cerebriformis described by moore in 1935, and maintained since then on sabouraud's agar in the mycology collection of the instituto de medicina tropical de são paulo. after 60 years, the samples exhibited the same characteristics described by moore (1935). however, experimental lesions did not resulted in guinea-pigs inoculated intratesticul ... | 1997 | 9460253 |
| paracoccidioidomycosis: an uncommon localization in the scrotum. | paracoccidioidomycosis is a systemic fungal infection caused by the thermally dimorphic fungus paracoccidioides brasiliensis. the authors present a case of a 49-year-old man who developed verrucous lesions on nasal mucosa and on genital localization (inguinal and scrotal), without signs of systemic disease. direct mycological examination, culture on sabouraud glucose medium, and biopsy were positive for p. brasiliensis. the interest of this case is the genital localization which is uncommon in t ... | 1997 | 9470405 |
| molecular cloning and sequencing of a chitin synthase gene (chs2) of paracoccidioides brasiliensis. | the nucleotide sequence of a chitin synthase gene (chs2) of the dimorphic fungal human pathogen paracoccidioides brasiliensis has been determined. the deduced amino acid sequence of chs2p consists of 1043 residues and is highly homologous to other class ii fungal chitin synthases. computational structural analyses suggest very high similarity to other fungal chitin synthases with a highly variable region at the cytosolic amino-terminal region which may be related to its possible zymogenic nature ... | 1998 | 9483806 |
| anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen paracoccidioides brasiliensis. | the surface anionogenic groups and sialoglycoconjugate structures of paracoccidioides brasiliensis yeast forms were analysed by cell microelectrophoresis, binding assays with lectins and viral particles, ultrastructural cytochemistry, enzymic digestion and flow cytofluorimetry. p. brasiliensis yeast forms, particularly the budding primordia, reacted strongly with cationized ferritin. binding assays showed that the reaction with sialic-acid-specific limax flavus lectin (lfa) was distributed over ... | 1998 | 9493368 |
| lung tissue mechanics in the early stages of induced paracoccidioidomycosis in rats. | pulmonary dysfunction represents the most important cause of death in patients with paracoccidioidomycosis (pbm). in order to investigate the functional changes of the lungs in the early stages of pbm, a model of benign disease was developed by intratracheal challenge of 12-week old isogenic wistar rats with 1 x 10(6) yeast forms of paracoccidioides brasiliensis. animals were studied 30 and 60 days after infection, when fully developed granulomas were demonstrable in the lungs. measurements of a ... | 1997 | 9496434 |
| short report: use of the polymerase chain reaction to detect paracoccidioides brasiliensis in murine paracoccidioidomycosis. | the polymerase chain reaction (pcr) was used to detect the presence of paracoccidioides brasiliensis in a murine model of disseminated paracoccidioidomycosis. using a previously identified p. brasiliensis-specific dna sequence, p. brasiliensis dna was detected in serum of five experimentally infected mice. the pcr method was able to detect as little as 10 pg of p. brasiliensis dna in serum, and it was more sensitive than blood culture isolation (five of five were pcr positive versus two of five ... | 1998 | 9502596 |
| electrophoretic karyotypes and genome sizing of the pathogenic fungus paracoccidioides brasiliensis. | here we present the karyotype analysis and genome sizing of paracoccidioides brasiliensis, a pathogen refractory to conventional genetic analysis. we have established pulsed-field gel electrophoresis (pfge) conditions to resolve the high-molecular-weight chromosomal bands of two clinical isolates of p. brasiliensis. both isolates showed four megabase-sized bands, ranging from 2.0 to 10.0 mbp. significant differences in chromosome sizes and in the chromosomal location of genes for the gp43 antige ... | 1998 | 9508305 |
| [production of fungal antigens from local strains for the immunodiagnosis of mycoses in mexico]. | fungal antigens with good reactivity and specificity are essential for the immunodiagnosis of systemic mycoses. we give here data on crude and purified antigens of histoplasma capsulatum, coccidioidis immitis and paracoccidioides brasiliensis from local strains and which are the more prevalent in mexico. the crude antigens had good reactivity in precipitating and skin testing whereas the purified antigens (dppc: deproteinized polysaccharide protein complex) had a higher specificity in more sensi ... | 1997 | 9528311 |
| experimental pulmonary fibrosis induced by paracoccidioides brasiliensis conidia: measurement of local host responses. | pulmonary fibrosis was induced following inoculation of paracoccidioides brasiliensis conidia intranasally in balb/c mice. fibrosis was associated with formation of granulomas, increase in lung hydroxyproline, and sustained increases in tissue tumor necrosis factor-alpha and transforming growth factor-beta. this study suggests a role for these cytokines in generation of pulmonary fibrosis associated with chronic granulomatous infectious diseases. | 1998 | 9574786 |
| isolation of paracoccidioides brasiliensis from armadillos (dasypus noveminctus) captured in an endemic area of paracoccidioidomycosis. | paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis (pcm), was first isolated from armadillos from the amazonian region where the mycosis is uncommon. in the present study, we report on the high incidence of pcm infection in armadillos from a hyperendemic region of the disease. four nine-banded armadillos (dasypus novemcinctus) were captured in the endemic area of botucatu, sao paulo, brazil, killed by manual cervical dislocation and autopsied under sterile conditions. f ... | 1998 | 9574800 |
| interactions of f1 fractions from different strains of paracoccidioides brasiliensis with human complement and with human neutrophils. | the aim of our study was to investigate differences that might exist in the activation of the human complement system by f1 fractions from four different isolates of p. brasiliensis. isolates hc and 18 (virulent), 265 (low virulence), and 9 (intermediate virulence, attenuated) were used; before the experiments, the virulence of isolates hc and 18 was recovered by in vivo passage in guinea pigs. the four isolates of the fungus were processed for purification of f1 fractions and the activation of ... | 1997 | 9608721 |
| palpebral paracoccidioidomycosis. | this paper describes two cases of eyelid paracoccidioidomycosis (south american blastomycosis) in which it was the first signal of the disease. in both cases the first clinical diagnosis made was not a fungal infection, but a neoplastic disease that was not confirmed by the pathology study. in the first patient we suspected a primary infection on the eyelid, because there was no other systemic signs of the disease, and in the second patient we noted a very advanced pulmonary lesions caused by th ... | 1997 | 9608722 |
| detection of circulating paracoccidioides brasiliensis antigen in urine of paracoccidioidomycosis patients before and during treatment. | for the diagnosis and follow-up of paracoccidioidomycosis patients undergoing therapy, we evaluated two methods (immunoblotting and competition enzyme immunoassay) for the detection of circulating antigen in urine samples. a complex pattern of reactivity was observed in the immunoblot test. bands of 70 and 43 kda were detected more often in urine samples from patients before treatment. the immunoblot method detected gp43 and gp70 separately or concurrently in 11 (91.7%) of 12 patients, whereas t ... | 1998 | 9620407 |
| geographic discrimination of paracoccidioides brasiliensis strains by randomly amplified polymorphic dna analysis. | randomly amplified polymorphic dna (rapd) analysis of 33 paracoccidioides brasiliensis strains from argentina, brazil, colombia, peru, and venezuela produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. types generated with five primers (opg 03, opg 05, opg 14, opg 16, and opg 18) resulted in a high discriminatory index (0.956). the discriminatory index was slightly reduced (0.940) when only two primers (opg 3 and opg 14) were u ... | 1998 | 9620409 |
| structure elucidation of sphingolipids from the mycopathogen paracoccidioides brasiliensis: an immunodominant beta-galactofuranose residue is carried by a novel glycosylinositol phosphorylceramide antigen. | two major acidic glycolipid components (pb-1 and pb-2) have been extracted from the mycopathogen paracoccidioides brasiliensis, a thermally dimorphic fungus endemic to rural areas of south and central america. sera of all patients exhibiting paracoccidioidomycosis were found to be reactive with pb-1, but not with pb-2; no reactivity was observed with sera of healthy patients or those with histoplasmosis [toledo, m. s., suzuki, s., straus, a. h., and takahashi, h. k. (1995) j. med. vet. mycol. 33 ... | 1998 | 9628738 |
| cytosolic neutral proteinases of paracoccidioides brasiliensis. | cytosolic proteinases were assayed in both morphological phases of paracoccidioides brasiliensis. preparations from the mycelial phase were more active in vitro than those from the yeast cells. optimal proteinase activities for both phases occurred at ph's between 6.0 and 9.0, and at 45 degrees c. gelatin-sds-page electrophoresis separated several bands (58-112 kda) in mycelial preparations; a single band (70 kda) was seen in yeast preparations. enzymatic activities were inhibited by antipain, p ... | 1998 | 9662616 |
| localization of eosinophil granule major basic protein in paracoccidioidomycosis lesions. | paracoccidioidomycosis is a chronic granulomatous disease caused by the fungus paracoccidioides brasiliensis. although eosinophils have long been associated with the immune defense against helminths, the role of eosinophils in the immune response to fungal diseases is not as well studied. the eosinophil granule major basic protein is toxic to helminths and mammalian cells in vitro, and its release has been used as a marker of eosinophil localization and degranulation. to determine whether eosino ... | 1998 | 9684630 |
| cytokines produced by susceptible and resistant mice in the course of paracoccidioides brasiliensis infection. | paracoccidioidomycosis (pcm) is the most prevalent deep mycosis in latin america and presents a wide spectrum of clinical manifestations. we established a genetically controlled murine model of pcm, where a/sn mice develop an infection which mimics the benign disease (immune responses which favor cellular immunity) and b10.a animals present the progressive disseminated form of pcm (preferential activation of b cells and impairment of cellular immune responses). to understand the immunoregulatory ... | 1998 | 9698765 |
| immunity to paracoccidioides brasiliensis infection. | 1998 | 9720958 | |
| molecular genetic variation in emmonsia crescens and emmonsia parva, etiologic agents of adiaspiromycosis, and their phylogenetic relationship to blastomyces dermatitidis (ajellomyces dermatitidis) and other systemic fungal pathogens. | emmonsia crescens, an agent of adiaspiromycosis, blastomyces dermatitidis, the agent of blastomycosis, and histoplasma capsulatum, the agent of histoplasmosis, are known to form meiotic (sexual) stages in the ascomycete genus ajellomyces (onygenaceae, onygenales), but no sexual stage is known for e. parva, the type species of the genus emmonsia. to evaluate relationships among members of the putative ajellomyces clade, large-subunit ribosomal and internal transcribed spacer region dna sequences ... | 1998 | 9738044 |
| treatment of paracoccidioides brasiliensis-infected mice with a nitric oxide inhibitor prevents the failure of cell-mediated immune response. | the activation of the nitric oxide (no) production system and its involvement in the control of the lung fungal burden and in immunosuppression mechanisms were studied during the course of paracoccidioides brasiliensis-infected mice. mice that had been infected with the fungus were treated daily with a specific inhibitor of no synthesis, n omega-nitro-l-arginine, or with buffered saline (control); no production was assessed on the basis of spontaneous no2- production by bronchoalveolar and perit ... | 1998 | 9743371 |
| study of pulmonary experimental paracoccidioidomycosis by analysis of bronchoalveolar lavage cells: resistant vs. susceptible mice. | adult swiss (susceptible) and balb/c (non-susceptible) mice were inoculated by the intravenous route with 1 x 10(6) yeast cells of paracoccidioides brasiliensis, strain 18. immunologic parameters, histopathology and features of the bronchoalveolar lavage (bal) were evaluated at week 2, 4, 8 and 16 post-infection. the pulmonary infection was progressive in swiss mice and regressive in balb/c mice. the numbers of total cells, lymphocytes and polymorphonuclear neutrophils increased in bal, as well ... | 1998 | 9750339 |
| evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of paracoccidioides brasiliensis in both mice and humans. | paracoccidioidomycosis (pcm) is a systemic mycosis endemic in latin america, with a high prevalence in brazil, argentina, colombia and venezuela. the aetiologic agent of disease is a thermal dimorphic fungus, paracoccidioides brasiliensis. a glycoprotein of 43,000 d (gp43) is the major antigen of p. brasiliensis. antibodies directed to this antigen are detected in the sera of all patients with pcm. gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. as ... | 1998 | 9764601 |
| antigenemia in patients with paracoccidioidomycosis: detection of the 87-kilodalton determinant during and after antifungal therapy. | serological diagnosis and follow-up of paracoccidioidomycosis (pcm) patients have relied mainly on the detection of antibody responses by using techniques such as complement fixation (cf) and immunodiffusion. we recently described a novel inhibition enzyme-linked immunosorbent assay (inh-elisa) which proved to be useful in the diagnosis of pcm via the detection of an 87-kda determinant in patient sera (b. l. gomez, j. i. figueroa, a. j. hamilton, b. ortiz, m. a. robledo, r. j. hay, and a. restre ... | 1998 | 9774584 |
| isolation of a paracoccidioides brasiliensis strain from the soil of a coffee plantation in ibiá, state of minas gerais, brazil. | paracoccidioides brasiliensis has rarely been isolated from its habitat in rural areas. in order to investigate the hypothesis that human infection with this fungus is linked to coffee plantations (coffea arabica), material was collected monthly over a period of 1 year from farms in the town of ibiá, state of minas gerais, brazil. a total of 760 samples of soil, coffee leaves and fruits was cultured and inoculated into mice. a fungus isolated from the liver of a mouse inoculated with soil showed ... | 1998 | 9776810 |
| lysis of paracoccidioides brasiliensis by zygosporium geminatum. | zygosporium geminatum, isolated as a contaminant in a culture of the mycelial phase of paracoccidioides brasiliensis, was lethal to the latter organism. its lytic action was due to exocellular alpha-1,3- and beta-1,3-glucanases which degraded the p. brasiliensis cell wall. the alpha-1,3-glucanase was more active at 30 degrees c and the beta-1,3-glucanase at 23 degrees c, each having ph 6.0 as its optimum. | 1998 | 9776817 |
| morphological transition of paracoccidioides brasiliensis conidia to yeast cells: in vivo inhibition in females. | clinical paracoccidioidomycosis is 13 times more common in men than in women. estrogen inhibits the transition of mycelia or conidia (the saprophytic form of paracoccidioides brasiliensis) to yeasts (the parasitic form) in vitro. here, we show that, in male mice that were infected intranasally (mimicking natural infection) the transition of conidia in bronchoalveolar lavage fluids to intermediate forms and yeasts occurred over 24 to 96 h; cfu and yeasts (shown by histopathology) increased subseq ... | 1998 | 9784579 |
| [renal anatomopathological changes in patients with acquired immunodeficiency deficiency syndrome]. | renal anatomopathological lesions were studied among 119 aids patients from faculdade de medicina do triângulo mineiro's university hospital (uberaba, mg, brazil). from formalin-fixed blocks, slides were obtained and studied by light microscopy. of 119 patients, 67 presented tubulointerstitial nephritis (tin), 18 inespecific, 2 xantogranulomatous and infections agents were found in 48 as follows: mycosis in 28 (16 cryptococcus sp; 9 histoplasma sp, 1 candida sp e 2 paracoccidioides brasiliensis) ... | 1998 | 9789445 |
| [specific fungal antisera production in rabbits]. | a method for fungic antisera production against aspergillus fumigatus, histoplasma capsulatum, paracoccidioides brasiliensis and coccidioides immitis in rabbits was evaluated. intradermic via and antigen in the dilution used for routine tests (ud) were employed to produce positive control serum for immunodiffusion test in agar gel. a. fumigatus, h. capsulatum and c. immitis antigens were prepared as described in cdc's procedure manual, p. brasiliensis antigen was prepared as previously described ... | 1998 | 9793140 |
| use of the 27-kilodalton recombinant protein from paracoccidioides brasiliensis in serodiagnosis of paracoccidioidomycosis. | paracoccidioidomycosis (pcm) is one of the most important endemic mycoses in latin america; it is usually diagnosed by observation and/or isolation of the etiologic agent, paracoccidioides brasiliensis, as well as by a variety of immunological methods. although the latter are effective, two circumstances, cross-reactions with other mycotic agents and antigen preparation that is marked by extreme variability among lots, hinder proper standardization of the procedures. to circumvent this lack of r ... | 1998 | 9801343 |
| pattern of immune response to gp43 from paracoccidioides brasiliensis in susceptible and resistant mice is influenced by antigen-presenting cells. | paracoccidioidomycosis (pcm), endemic in latin america, is a progressive systemic mycosis caused by paracoccidioides brasiliensis. the infection can evolve to different clinical forms that are associated with various degrees of suppressed cell-mediated immunity. in the murine model, a/sn and b10.a isogenic strains of mice are known to be resistant and susceptible, respectively, to this fungal infection. assuming that the effector immune response is a consequence of the preferential activation of ... | 1998 | 9826448 |
| virulence factors in fungi of systemic mycoses. | pathogenic fungi that cause systemic mycoses retain several factors which allow their growth in adverse conditions provided by the host, leading to the establishment of the parasitic relationship and contributing to disease development. these factors are known as virulence factors which favor the infection process and the pathogenesis of the mycoses. the present study evaluates the virulence factors of pathogenic fungi such as blastomyces dermatitidis, coccidioides immitis, cryptococcus neoforma ... | 1998 | 9830725 |
| paracoccidioidomycotic aortitis with embolization to the lower limbs. report of a case and review of the literature. | the authors report a clinical case of a 60-year-old caucasian man, with two episodes of arterial embolization in the lower limbs. a microscope investigation of the emboli revealed that they originated from fungal aortitis caused by paracoccidioides brasiliensis. a review of aortic infections showed only one similar report of this rare clinical expression of blastomycosis. the authors suggest a routine postoperative search for emboli followed by culture and histopathology. | 1998 | 9833714 |
| comparative studies on the antibody repertoire produced by susceptible and resistant mice to virulent and nonvirulent paracoccidioides brasiliensis isolates. | the specific recognition pattern of antibodies produced by susceptible and resistant mice infected with the low virulence paracoccidioides brasiliensis isolate (pb265) was examined by an immunoblotting procedure and compared with that of antibodies produced by highly virulent isolate (pb18) in infected mice. both mouse strains produced igg antibodies to 13 of the 16 major antigen bands, and showed a recognition pattern similar to sera from mice infected with the virulent isolate. nevertheless, t ... | 1998 | 9886208 |
| [the research encouragement award. effects of sex hormones on sexual difference of experimental paracoccidioidomycosis]. | paracoccidioidomycosis is a deep mycosis in latin america. the causative agent is paracoccidioides brasiliensis, a thermal dimorphic fungus. the incidence of the disease is much higher in men than in women. although one explanation is the inhibitive effect of estradiol on fungal growth, its effect on the yeast form growth of p. brasiliensis is still unclear. there is limited information on progesterone and testosterone, which shows weak or no effects. on the other hand, numerous studies on sexua ... | 1999 | 9929575 |
| stress proteins in fungal diseases. | heat shock proteins (hsps) are ubiquitous families of proteins, found in all organisms studied so far. they are highly conserved across the species barrier and serve fundamental functions in cell physiology. the term 'heat shock' was adopted because of the early observation of the heat-inducible nature of these proteins, although, as it is now realized that they can be induced by a variety of stressful stimuli, it is probably more appropriate to call them 'stress proteins'. the nomenclature of m ... | 1998 | 9988491 |
| development of vaccines and their use in the prevention of fungal infections. | vaccine approaches to infectious diseases are widely applied and appreciated. disciplines such as bacteriology and virology have a rich history of successful vaccine development. the complexity of eukaryotic systems presents additional challenges to the development of vaccines against them. these challenges are being met in the fields of parasitology, and are being revisited for application in oncology. vaccine opportunities exist in medical mycology. the national institute of allergy and infect ... | 1998 | 9988493 |
| isolation of fungal rdna from bottlenose dolphin skin infected with loboa loboi. | fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (pcr) to amplify dna from lobomycosis lesions in a bottlenose dolphin. sequence alignments of this dna possessed high homology to fungal ribosomal dna sequences found in the genus cladosporium. when used for in situ hybridization, the riboprobe transcribed from a cloned pcr-generated fragment bound to loboa loboi cells. these results support the hypothesis that l. loboi i ... | 1998 | 10075494 |
| exocellular proteolytic activity of paracoccidioides brasiliensis: cleavage of components associated with the basement membrane. | we have previously characterized an exocellular serine-thiol proteinase activity in paracoccidioides brasiliensis, using as substrates peptides analogous of the internally quenched fluorogenic peptide abz-mkrltl-eddnp. in this communication, detection of maximal proteinase activity in the culture supernatant fluids followed the abrupt increase in the medium ph, owing to the accumulation of ammonia generated by urease activity. culture supernatant fluids collected at the peak of proteinase activi ... | 1998 | 10075506 |