Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antigenic structure of paracoccidioides brasiliensis. | 1989 | 2490077 | |
| hypersensitivity pneumonitis to paracoccidioides brasiliensis antigens in mice. | hypersensitivity pneumonitis was induced in mice immunized with paracoccidioides brasiliensis and challenged, one week later, with soluble (spbag) or particulate (ppbag) antigen (formalin-killed yeast cells), administered by the intratracheal route. between 24 and 48 h post-challenge, animals developed an interstitial and intra-alveolar pneumonitis. macrophages and lymphocytes arranged focally into loose or mature granulomata were observed by light and electron microscopy. the distribution and f ... | 1989 | 2501469 |
| in situ localization of t lymphocyte subsets in human paracoccidioidomycosis. | immunohistochemical techniques using monoclonal antibodies to t lymphocyte subsets were used to characterize granulomas caused by paracoccidioides brasiliensis. punch biopsies of skin or mucosa from eight patients and of lymph nodes from two patients with disseminated paracoccidioidomycosis were studied. the t lymphocytes were distributed either in a localized pattern related to epithelioid granulomas or in a diffuse arrangement, predominantly around the vessels. in the granulomas, t cells forme ... | 1989 | 2528613 |
| mode of action of itraconazole: morphological aspects. | the broad spectrum of antifungal activity of itraconazole is verified by morphologic criteria at the light and electron microscopical level. yeast and fungal species examined are candida albicans, cryptococcus neoformans, paracoccidioides brasiliensis, sporothrix schenckii, pityrosporum ovale, trichophyton rubrum and aspergillus fumigatus. exposures of cultures of these yeasts and fungi to itraconazole results in dose- and time-dependent alterations which vary in nature and intensity from one sp ... | 1989 | 2561185 |
| detection of the 43,000-molecular-weight glycoprotein in sera of patients with paracoccidioidomycosis. | the 43,000-molecular-weight (43k) soluble glycoprotein was detected in sera of patients with paracoccidioidomycosis by the immunoblot technique by using as the probe rabbit monospecific antisera to this fraction. the 43k antigen was present before treatment in sera of patients with the acute (juvenile) form; it started to disappear from circulation after 10 months of chemotherapy, and it was undetectable after 2 years of treatment. in the chronic cases, the 43k antigen was detected in patients w ... | 1989 | 2592544 |
| influence of oestradiol on protein expression and methionine utilization during morphogenesis of paracoccidioides brasiliensis. | the temporal sequence of cytosolic protein expression during phase transition of paracoccidioides brasiliensis was examined. electrophoretic analysis of cytosol proteins by one-dimensional sds-page revealed numerous differences between the mycelial and yeast forms as well as alterations induced by 17 beta-oestradiol. using either protein staining or fluorography of [35s]methionine-labelled proteins 30 phase-specific bands were detected, 12 mycelial-associated bands (range 30 to 140 kda) and 18 y ... | 1989 | 2614390 |
| chlamydospore formation by paracoccidioides brasiliensis mycelial form. | to investigate the role of some adverse environmental conditions in chlamydospore formation by the mycelial form of p. brasiliensis, we cultured four p. brasiliensis isolates (18, bt4, 1183, pb9) at 25 degrees c within solid agar medium either rich or poor in nutrients. isolates 18 and 1183 were also cultured under anaerobiosis in a nitrogen atmosphere. isolate 18 produced great number of terminal and intercalary chlamydospore after 7-10 days of culture in a medium poor in nutrients (2% agar wit ... | 1989 | 2617011 |
| functions of polymorphonuclear leukocytes and individuality of jorge lobo's disease: absence of the specific leukocyte digestive defect against paracoccidioides brasiliensis. | peripheral blood neutrophils (pmns) from a patient with jorge lobo's disease (jld) digested well phagocytosed paracoccidioides brasiliensis. we found no circulating antibodies against p. brasiliensis in the patient's serum. such neutrophils showed myeloperoxidase activity and also digested normally phagocytosed candida albicans. we had previously reported the presence of a specific digestive deficiency of pmns from patients with paracoccidioidomycosis (para) vis à vis p. brasiliensis. current fi ... | 1989 | 2622474 |
| identification of antigenic polypeptides of paracoccidioides brasiliensis by immunoblotting. | exoantigens from cultures of paracoccidioides brasiliensis were separated by sds-page and subsequently blotted onto nitrocellulose paper. immunologically reactive components were detected by reaction with sera from patients with paracoccidioidomycosis (pcm). peroxidase-conjugated anti-human igg was used as a detector antibody to reveal immunocomplexes. anti-p. brasiliensis igg reacted with four major components of 70, 52, 43 and 20-21 kda and with eight minor components. the 43 kda glycoprotein ... | 1989 | 2628560 |
| dimorphism in histoplasma capsulatum: a model for the study of cell differentiation in pathogenic fungi. | several fungi can assume either a filamentous or a unicellular morphology in response to changes in environmental conditions. this process, known as dimorphism, is a characteristic of several pathogenic fungi, e.g., histoplasma capsulatum, blastomyces dermatitidis, and paracoccidioides brasiliensis, and appears to be directly related to adaptation from a saprobic to a parasitic existence. h. capsulatum is the most extensively studied of the dimorphic fungi, with a parasitic phase consisting of y ... | 1989 | 2666842 |
| the interface of mycology and endocrinology. | message molecules such as hormones or pheromones have been described in non-pathogenic fungi and largely control mating. in one instance, a fungal hormone has a sequence homologous with a mammalian hormone, binds to a mammalian receptor and produces a functional response in a mammalian cell. some mammalian hormones have also been shown to inhibit or stimulate pathogenic and non-pathogenic fungi, although until recently fungal binders (receptors) had not been demonstrated and the concentrations s ... | 1989 | 2674385 |
| [paracoccidioidomycosis and infection by the human immunodeficiency virus]. | we present two cases of paracoccidioidomycosis, one occurring in an aids patient and the other in an hiv infected man. this is the first report of such association. the first patient, which was already followed for hiv infection (group iv-a) presented with high fever and hepatosplenomegaly. plain x-ray, ultrasound and ct-scan of the abdomen showed solid nodules in the spleen, some of them with calcification. both the direct smear and the culture of a bone marrow aspiration revealed paracoccidioi ... | 1989 | 2690307 |
| histological and ultrastructural study of the inflammation evoked by paracoccidioides brasiliensis antigen in previously immunized mice. | bentonite particles uncoated and coated with soluble antigen of paracoccidioides brasiliensis (pb) were intravenously injected into mice with and without previous immunization with pb antigen. the inflammatory reaction around the bentonite emboli in small lung vessels was quantitated and morphologically studied by light and electron (em) microscopy, 2 to 8 days after challenge. in control nonimmunized animals, coated and uncoated bentonite particles caused mild and nonspecific inflammation made ... | 1989 | 2739693 |
| intracellular multiplication of paracoccidioides brasiliensis in macrophages: killing and restriction of multiplication by activated macrophages. | the effect of coculturing yeast-form paracoccidioides brasiliensis with murine cells was studied. coculture of resident peritoneal or pulmonary macrophages with p. brasiliensis for 72 h dramatically enhanced fungal multiplication 19.3 +/- 2.4- and 4.7 +/- 0.8-fold, respectively, compared with cocultures with lymph node cells or complete tissue culture medium alone. support of p. brasiliensis multiplication by resident peritoneal macrophages was macrophage dose dependent. lysates of macrophages, ... | 1989 | 2744848 |
| evaluation of the pathogenicity and immunogenicity of seven paracoccidioides brasiliensis isolates in susceptible inbred mice. | in this investigation the pathogenicity and immunogenicity of seven isolates of paracoccidioides brasiliensis were compared. the pathogenicity of each isolate was determined by 50% lethal dose estimations and histopathology analysed during a 24-week-period. four basic patterns of virulence could be defined after intraperitoneal infection of susceptible, genetically homogenous, b10.a mice, namely, slightly virulent (isolates pb 265 and ivic pb 267), intermediate (isolates pb 192, ivic pb 9 and pb ... | 1989 | 2746437 |
| [infection caused by paracoccidioides brasiliensis in people living in cocle and veraguas, republic of panamá]. | intradermal application of yeast phase paracoccidioidin antigen obtained by sonication and lysis from the fungus to 111 residents of veraguas province (68 men and 43 women) and 130 cocle province residents (57 men and 73 women) elicited a positive reaction in 9.01% (1/111) and 26.15% (34/30) of the veraguas and cocle residents respectively. positive reactions ranged between papules of 5 and 27 mm in diameter, the majority being between 11 and 20 mm. infection was more frequent in men (1:1.47 for ... | 1989 | 2762594 |
| digestion of killed paracoccidioides brasiliensis by neutrophils. | we previously described an in vitro assay showing that neutrophils (pmns) from patients with paracoccidioidomycosis (para) have a specific digestive deficiency against suspensions of live paracoccidioides brasiliensis. we now report that this defect is equally detectable against autoclaved, but not amphotericin b-killed p. brasiliensis. the use of autoclaved suspensions facilitates the use of our in vitro assay. it might allow the development of an in vitro intradermal test for digestion of fung ... | 1989 | 2770840 |
| virulence of a variant of paracoccidioides brasiliensis that exists in the yeast form at room temperature. | paracoccidioides brasiliensis (atcc 60885) produces conidia that, when plated in enriched media and incubated at 21-25 degrees c, give rise to a yeast-form variant (yrt) (atcc 46678). the virulence of this variant for balb/c mice has been compared with that of the yeast and conidia produced by the parent isolate. adult female mice were inoculated intravenously with 10(7) viable cells of yrt or yeast, or 5 x 10(5) viable conidia. mice were weighed weekly, and three mice were sacrificed at 1, 2, 3 ... | 1989 | 2778575 |
| the possible role of circulating immune complexes in the deficiency of cell-mediated immunity in paracoccidioidomycosis. | 1. the nature and extent of immune abnormalities was studied in 28 untreated patients with a chronic moderate form of paracoccidioidomycosis (pcm). 2. the patients presented hyporeactivity to skin tests, diminished lymphocyte transformation by mitogens such as phytohemagglutinin-p and concanavalin a and by paracoccidioides brasiliensis protein antigen. they also presented peripheral blood leukocytosis but normal absolute numbers of t-cell and t-cell subsets. 3. the patients had increased serum l ... | 1989 | 2790289 |
| suppression of ige antibody production against an unrelated antigen in experimental murine paracoccidioidomycosis. | a suppression of the ige antibody response to ovalbumin was obtained in susceptible mice infected with paracoccidioides brasiliensis yeast cells a few days prior to immunization with the former antigen plus adjuvant. a direct relationship between the number of injected fungi and the suppressive effect was established. when infection with a pathogenic isolate of p. brasiliensis (pb 18) was compared to a non-pathogenic isolate (ivic pb267), the ige anti-ovalbumin response was reduced by both. a si ... | 1989 | 2795403 |
| evaluation of cilofungin, a lipopeptide antifungal agent, in vitro against fungi isolated from clinical specimens. | cilofungin (ly121019) is a new lipopeptide antifungal drug. we tested this drug against 141 pathogenic fungal isolates. all fungal species were tested by broth dilution at 35 degrees c. malassezia furfur was tested by agar dilution. the results demonstrate the specificity of cilofungin activity. candida albicans, candida tropicalis, and malassezia pachydermatis were highly susceptible, whereas candida parapsilosis, candida pseudotropicalis, candida krusei, torulopsis glabrata, blastomyces dermat ... | 1989 | 2802565 |
| [prevalence of paracoccidioides brasiliensis infection in panamanian children. preliminary report]. | the prevalence of paracoccidioides brasiliensis infection in panamanian children was demonstrated to be 12.73% (14/110) by skin reactivity to 50 micrograms of yeast derived antigen. boys were infected more frequently than girls - 1.45:1. the earliest age of infection was 2 years, and a direct relationship was noted between age and frequency of infections (as the children became older, more of them became infected); boys more so than girls. thirteen of 14 (93%) of the infected juvenile population ... | 1989 | 2813876 |
| inhibition of growth of the dimorphic fungus paracoccidioides brasiliensis by ajoene. | ajoene, a garlic-derived compound that prevents platelet activation, inhibited the growth of paracoccidioides brasiliensis, a fungal pathogen for humans, by affecting the integrity of the fungal cytoplasmic membrane. this action may be the basis for the study of ajoene as a possible specific antifungal drug. | 1989 | 2817865 |
| production and characterization of murine monoclonal antibodies to histoplasma capsulatum yeast cell antigens. | four monoclonal antibodies (mabs) were produced by immunizing mice with a disrupted yeast cell homogenate of histoplasma capsulatum. mabs 1 and 2 reacted only with the yeast cell antigens of h. capsulatum and blastomyces dermatitidis, whereas mabs 3 and 4 showed broader cross-reactivity. mab 3 cross-reacted with b. dermatitidis, paracoccidioides brasiliensis, sporothrix schenckii, and candida albicans, and mab 4 cross-reacted with b. dermatitidis, c. albicans, coccidioides immitis, aspergillus f ... | 1989 | 2917790 |
| induction of antigen-specific t suppressor cells by soluble paracoccidioides brasiliensis antigen. | in naturally acquired paracoccidioidomycosis, patients have depressed in vivo and in vitro cell-mediated immune (cmi) responses to paracoccidioides brasiliensis antigen. in addition, it has been reported that these patients have significant levels of circulating paracoccidioidal antigen in their sera. the primary purpose of this investigation was to assess the effects of p. brasiliensis antigen on the cmi responses in a mouse model. on the basis of findings with other fungal agents, we predicted ... | 1988 | 2964411 |
| characterization of efferent t suppressor cells induced by paracoccidioides brasiliensis-specific afferent t suppressor cells. | previously, we reported that paracoccidioides brasiliensis culture filtrate antigen (pb.ag) when injected i.v. into mice induces antigen-specific suppressor cells which down-regulate the anti-p. brasiliensis delayed-type hypersensitivity (dth) response. the suppressor cells are present in both spleens and lymph nodes of pb.ag-treated animals and suppress the afferent limb but not the efferent limb of the dth response to p. brasiliensis. the suppressor cells induced by pb.ag are l3t4+ lyt-1+2- i- ... | 1988 | 2964412 |
| regulation of immune responses by t suppressor cells and by serum in chronic paracoccidioidomycosis. | regulation of cellular responses was studied during the course of chronic murine disseminated paracoccidioidomycosis. regulation of peripheral blood lymphocyte (pbl) proliferative responses to concanavalin a (con a) was studied in vitro by mixing pbl from infected and noninfected mice. pbl from mice infected for 18 weeks had depressed responses to con a and they depressed the con a responses of pbl from noninfected mice by 95% when they were mixed in a 1:1 ratio. after treatment of pbl from infe ... | 1988 | 2972392 |
| a culture medium for paracoccidioides brasiliensis with high plating efficiency, and the effect of siderophores. | the plating efficiency of paracoccidioides brasiliensis on standard mycological media is poor, impairing its isolation and recovery from various sources, particularly infected tissues. we describe a medium that markedly improves p. brasiliensis plating efficiency. it consists of a synthetic medium (modified mcveigh-morton) supplemented with 4% (v:v) horse serum and 5% (v:v) culture filtrate from stationary phase p. brasiliensis cultures. a commercially available medium (brain-heart infusion), or ... | 1988 | 2977619 |
| cyclic adenosine 3',5' monophosphate (camp) and dimorphism in the pathogenic fungus paracoccidioides brasiliensis. | exogenous camp or its analogs inhibit the mycelium transformation of yeast and induce bulging of the apex of mycelia. but intracellular camp levels of yeast and mycelial cells are not significantly different. | 1985 | 3001525 |
| treatment of paracoccidioidomycosis with itraconazole in a murine model. | a new triazole, itraconazole, was studied as oral therapy of paracoccidioidomycosis in a murine model. the paracoccidioides brasiliensis isolate, susceptible to itraconazole in vitro, was given by intranasal challenge, producing acute pulmonary and disseminated disease. therapy was given twice daily over 4 weeks, and animals observed over 2 months. the infection was lethal for 70-80% of controls (untreated or polyethylene glycol diluent), whereas all treated animals, given 10-200 mg kg-1 day-1, ... | 1985 | 3014164 |
| effect of nucleotides on glucan synthesis in paracoccidioides brasiliensis. | the activity of paracoccidioides brasiliensis glucan synthetase was partially inhibited by guanosine 5'-triphosphate, adenosine 3'5'-phosphate and adenosine 5'-triphosphate. this inhibition was more pronounced in the mycelial system than in the yeast one, being higher at 23 degrees c than at 37 degrees c. addition of ethene diamine tetracetic acid to the incubation mixture inhibited partially the enzymatic activity in mycelial preparations but stimulated it in the yeast system. | 1986 | 3016229 |
| degenerative changes in fungi after itraconazole treatment. | changes in morphogenetic behavior and structural degeneration after exposure to itraconazole are illustrated in candida albicans, cryptococcus neoformans, pityrosporum ovale, paracoccidioides brasiliensis, trichophyton rubrum, and aspergillus fumigatus. with the exception of p. ovale, primary alterations are seen at the cell periphery and the cytoplasmic vacuoles in which lipid-like vesicles assemble. these changes are usually accompanied by a marked increase in cell volume, impaired cell divisi ... | 1987 | 3027844 |
| systemic fungal infections: diagnosis and treatment. i. paracoccidioidomycosis. | paracoccidioidomycosis is the designation for the disease caused by the dimorphic fungus paracoccidioides brasiliensis. it is a disease of great importance in latin america and constitutes an important diagnosis for consideration in patients from endemic areas who have pulmonary or systemic disease compatible with fungal infection. in the laboratory, basic work has uncovered important interactions between the fungus and the human sex steroid system. these studies may have important implications ... | 1988 | 3062094 |
| crossed-antigenicity between the etiologic agents of lobomycosis and paraccocidioidomycosis evidenced by an immunoenzymatic method (pap). | lobomycosis or keloidal blastomycosis and paracoccidiodomycosis found in south american tropical regions are considered different mycoses from the clinical and mycological point of view. very little is known about the immunology of lobomycosis, since the etiological agent of this mycosis, loboa loboi, is not cultured "in vitro". in order to overcome this difficulty, a rabbit paracoccidioides brasiliensis, antiserum is usually used, since crossed-antigenicity between both fungi has been demonstra ... | 1988 | 3067565 |
| [acute inflammation of a corneal graft caused by paracoccidioides brasiliensis--a case report]. | 1988 | 3071475 | |
| estradiol-binding proteins from mycelial and yeast-form cultures of paracoccidioides brasiliensis. | paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, causes disease much more frequently in men than it does in women, suggesting that the hormonal milieu of the host might influence p. brasiliensis pathogenicity. we recently demonstrated that cytosol from yeast cultures of p. brasiliensis contains a high-affinity, low-capacity, tritiated 17 beta-estradiol [( 3h]estradiol)-binding protein. estradiol and, to a lesser degree, diethylstilbestrol (des), inhibited the transfo ... | 1986 | 3079729 |
| a comparative study of the immunoantigenicity of eight paracoccidioides brasiliensis isolates. | 1986 | 3108996 | |
| in vivo and in vitro activation of pulmonary macrophages by ifn-gamma for enhanced killing of paracoccidioides brasiliensis or blastomyces dermatitidis. | the fungicidal capacity of murine pulmonary macrophages (pum) activated in vitro with ifn or lymphokines or in vivo with ifn was studied. pum treated overnight with ifn (1000 u/ml), con a-stimulated spleen cell culture supernatants, or lymph node cells plus con a significantly killed yeast cells of the gar w isolate of paracoccidioides brasiliensis 45.5 +/- 2.1%, 72.0 +/- 4.2%, and 51.5 +/- 0.7% respectively. two other isolates of p. brasiliensis (ru and la) were also killed (45 and 34%) by pum ... | 1988 | 3128610 |
| impairment of cellular but not humoral immune responses in chronic pulmonary and disseminated paracoccidioidomycosis in mice. | humoral and cellular immune responses were measured during the progression of chronic pulmonary and disseminated paracoccidioidomycosis in mice. the chronic disease was established by pulmonary infection of mice with different doses of the yeast form of paracoccidioides brasiliensis isolate gap. levels of antibodies to p. brasiliensis, detected in serum by immunodiffusion and enzyme-linked immunosorbent assay, directly correlated with the size of the infectious challenge. significant delayed-typ ... | 1988 | 3133318 |
| radiometric detection of metabolic activity of paracoccidioides brasiliensis and its susceptibility to amphotericin b and diethylstilbestrol. | 1987 | 3136531 | |
| production of paracoccidioides brasiliensis exoantigens for immunodiffusion tests. | growth curves of the yeast form of paracoccidioides brasiliensis b-339 based on total and viable cell counts were determined. crude culture filtrate antigens were obtained after 7, 10, 15, 20, 25, and 30 days of incubation. different patterns of proteins were obtained by affinity chromatography on sepharose 4b-immunoglobulin g complex made with immunoglobulin g from patients with paracoccidioidomycosis, with subsequent analyses by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and sca ... | 1988 | 3141460 |
| gamma-interferon activation of macrophages for killing of paracoccidioides brasiliensis and evidence for nonoxidative mechanisms. | fungicidal activity of murine peritoneal macrophages for the yeast form of the dimorphic fungal pathogen p. brasiliensis was studied. killing was assessed by reduction of colony forming units (cfu) using a new medium which has a good plating efficiency. resident peritoneal macrophages phagocytosed but did not kill p. brasiliensis. macrophages treated overnight with recombinant gamma-interferon (ifn), lymph node cells plus concanavalin a (con a) or con a-stimulated spleen cell culture supernatant ... | 1988 | 3145925 |
| cell walls from avirulent variants of histoplasma capsulatum lack alpha-(1,3)-glucan. | cell wall composition of isogenic virulent-avirulent strain pairs of histoplasma capsulatum varied markedly with respect to alpha-(1,3)-glucan content. when yeast cell walls were fractionated by standard techniques, the avirulent strains contained up to 1,000-fold less alpha-(1,3)-glucan than did their virulent parents. no alpha-(1,3)-glucan could be detected on the surface of the avirulent strain yeast cells if we used a mouse monoclonal antibody that recognized this polymer. a similar relation ... | 1988 | 3169995 |
| connective matrix organization in chronic granulomas of experimental paracoccidioidomycosis. | the histological and ultrastructural aspects of chronic granulomas from rats infected intraperitoneally with paracoccidioides brasiliensis are described with special emphasis on the composition of the extracellular matrix. the granulomas were structurally arranged in two zones, one central containing fungi, and the other peripheral. the extracellular matrix was composed of collagen types i and iii, proteoglycans, glycoprotein, and an undefined amorphous substance. the main cellular population wa ... | 1988 | 3173468 |
| [lobomycosis]. | lobomycosis is a rare disease occurring with predilection in the amazonian region. we report the clinical and histological presentation of a patient living in peru. the lesion resembled an hypertrophic scar located on the neck. it corresponded to a massive infiltration of the dermis by macrophages loaded with numerous loboa loboi. | 1988 | 3214501 |
| polymorphonuclear leukocyte functions in psoriasis. | circulating polymorphonuclear leukocyte (cpmn) functions were studied in patients with widespread psoriasis as well as in persons with chronic alcoholic liver disease (cald), paracoccidioidomycosis, diverse granulomatous diseases, and normal individuals. we were unable to find stimulation or increase in cpmn functions in patients with psoriasis compared to normal individuals. leukocytes from individuals with cald had a lowering of their metabolic activation, chemotaxis, random movement, and adhe ... | 1988 | 3229889 |
| morphological study of a variant of paracoccidioides brasiliensis that exists in the yeast form at room temperature. | incubation of paracoccidioides brasiliensis conidia at 20-25 degrees c (rt) results in two types of colonies, mycelial (m) and yeast (yrt). a study of the latter colonies was undertaken. conidia were plated in complex (bhi) and chemically-defined media (cdcm), with and without fetal calf serum (fcs). incubation was carried out at 21 degrees c for 4 weeks. the mean number of yrt colonies was approximately 18.5% on bhi agar. selected yrt colonies were transferred to liquid cdcm, incubated 7 days a ... | 1988 | 3236146 |
| [manifestation of a systemic mycosis (paracoccidioides brasiliensis) in the oral cavity]. | 1988 | 3240610 | |
| secretion of the 43 kda glycoprotein antigen by paracoccidioides brasiliensis. | yeast forms of the pathogenic fungus paracoccidioides brasiliensis secrete into the culture supernatant a 43,000 daltons glycoprotein (gp43) which can be immunoprecipitated specifically by sera from patients with paracoccidioidomycosis. we show here that following labelling of p. brasiliensis with (35s)methionine, gp43 was detected as the major component in the culture supernatant fluid as early as 1 hour after addition of the radiolabel. the amount of gp43, as determined by a competitive radioi ... | 1988 | 3246625 |
| immune response to paracoccidioides brasiliensis in human and animal hosts. | 1988 | 3288357 | |
| inhibition by estrogens of conidium-to-yeast conversion in the fungus paracoccidioides brasiliensis. | conidia produced by paracoccidioides brasiliensis are inhibited by mammalian estrogens in their in vitro conversion into yeast-form cells. this was demonstrated with four different isolates. in these experiments, conversion was reduced to 10.7 and 34.4% of the control values by 17-beta-estradiol at 10(-6) and 10(-8) m, respectively. at the same concentrations, the synthetic estrogen diethylstilbestrol was slightly less inhibitory. in contrast, other sex hormones and analogs, i.e., testosterone, ... | 1988 | 3343055 |
| paracoccidioidomycosis in nude mice: presence of filamentous forms of the fungus. | congenitally athymic nude mice (nu/nu) and their phenotypically normal littermates (nu/+) were intraperitoneally infected with yeast cells of a strain of paracoccidioides brasiliensis. the nude mice developed a severe and generalized infection with an intense parasitism of several organs, accompanied by a low-grade of tissue reaction. the lesions were characterized by abundant yeast-like cells of the fungus, and in some animals, numerous hyphal forms could be well visualized. in control animals, ... | 1988 | 3352721 |
| paracoccidioidomycosis: study of six cases with ocular involvement. | we present 6 patients with ocular involvement due to paracoccidioidomycosis. all cases were confirmed by the finding of paracoccidioides brasiliensis in histopathological or direct mycologic examination of material from the lesion in the eyelid or conjunctiva. in two cases the bulbar conjunctiva was also involved, in another the cornea, and still another patient developed endophthalmitis. the presence of this mutilating disease which may lead to blindness should be suspected when chronic blephar ... | 1988 | 3405277 |
| lipid composition and effect of amphotericin b on yeast cells of paracoccidioides brasiliensis. | yeast cells of paracoccidioides brasiliensis strain sn, were obtained for analysis of lipid composition. total lipids, phospholipids, sterols, and qualitative sterols and fatty acid composition were determined. such analysis were made on cells cultured in the presence or absence of amphotericin b and on non proliferating cell suspensions exposed to the antibiotic. marked alterations in lipid contents were observed in this different conditions. the major alterations were the reduction of total li ... | 1988 | 3405278 |
| growth and production of iron chelants by paracoccidioides brasiliensis mycelial and yeast forms. | the mycelial and yeast forms of the fungus paracoccidioides brasiliensis were cultured in a chemically defined liquid medium with different iron concentrations and the growth measured spectrophotometrically. the iron binding capacity of culture supernatants was measured by a colorimetric assay. both the mycelial and yeast forms were able to grow in media containing trace amounts of iron (0.02 mgl-1) but when the iron chelant 1.10 phenantroline was added there was a delay in the initiation of gro ... | 1988 | 3418467 |
| chronic pulmonary and disseminated paracoccidioidomycosis in mice: quantitation of progression and chronicity. | an animal model of chronic paracoccidioidomycosis was established in male adult balb/cbyjimr mice by intranasal instillation of different doses of yeast form paracoccidioides brasiliensis. the inoculum was standardized in terms of virulence, age of the culture, percentage of multicellular fungal units containing 1-3 cells, and viability. progression and chronicity of the infection was measured by quantitative counts of colony forming units (cfu) of p. brasiliensis from infected lungs, spleens, a ... | 1987 | 3437374 |
| experimental paracoccidioidomycosis: early immunosuppression occurs in susceptible mice after infection with pathogenic fungi. | the course of paracoccidioides brasiliensis infection depends on the natural resistance of the host and on the virulence of the fungus isolate. in the present study the immune response to sheep red blood cells (srbc) was investigated in susceptible (b10.a) and resistant (a/sn) mice after intraperitoneal (ip) infection with pathogenic (pb18) and apathogenic (ivic pb267) p. brasiliensis isolates. infection with pb18 yeast cells quickly induces a state of immunodepression only in the susceptible mi ... | 1987 | 3452450 |
| [a model of dimorphism in pathogenic fungi: paracoccidioides brasiliensis]. | 1987 | 3453569 | |
| effect of murine polymorphonuclear leukocytes on the yeast form of paracoccidioides brasiliensis [corrected]. | the fungicidal activity of murine polymorphonuclear neutrophils from the peripheral blood or elicited intraperitoneally with thioglycollate or with antigen in paracoccidioides brasiliensis-sensitized [corrected] or nonsensitized mice was studied. although peripheral blood, thioglycollate-elicited, and antigen-elicited neutrophils from normal mice or thioglycollate-elicited neutrophils from p. brasiliensis-sensitized [corrected] mice killed candida albicans (57% to 84%), they failed to significan ... | 1987 | 3555139 |
| the role of fractions from paracoccidioides brasiliensis in the genesis of inflammatory response. | the influx of inflammatory cells towards the peritoneal cavity in rats inoculated intraperitoneally with subcellular preparations of the fungus paracoccidioides brasiliensis was studied. in addition to the dead fungus, also fractions f1 of the cell wall, which mainly consisted of polysaccharides and the lipid extract, induced intense cell migration 4 hr after inoculation, with a greatly increased number of polymorphonuclear leucocytes (pmn). study of the kinetics of cell influx showed that both ... | 1987 | 3561470 |
| a comparative histopathological, immunological, and biochemical study of experimental intravenous paracoccidioidomycosis induced in mice by three paracoccidioides brasiliensis isolates. | we carried out a comparative study of the histopathology (lung, liver, spleen, kidney and adrenals) and the anti-p. brasiliensis humoral (immunodiffusion test) and cellular (footpad test) immune response of mice intravenously inoculated with yeast forms of three p. brasiliensis isolates (pb 18, pb 192, pb 265). pb 265 (avirulent strain) did not evoke specific lesions or antibody production; the levels of cellular immunity were significantly lower than those of the two other isolates. lung granul ... | 1986 | 3572679 |
| an evaluation of the enzyme-linked immunoabsorbent assay (elisa) for quantitation of antibodies to paracoccidioides brasiliensis. | the elisa procedure was adapted for quantitation of antibodies against paracoccidioides brasiliensis. using a yeast cytoplasmic antigen and sera from patients with proven paracoccidioidomycosis, we found that 66% of sera reacted at titers greater than or equal to 1:128. titers of this magnitude were obtained only for 4-5% of sera from healthy blood donors, tuberculosis patients and patients with other systemic mycoses. the exception was sera from patients with histoplasmosis (36% had titers grea ... | 1986 | 3572681 |
| a case of pseudoparacoccidioidomycosis: detection of the yeast phase of mucor circinelloides in a clinical specimen. | a case is presented in which yeastlike cells resembling the yeast phase of paracoccidioides brasiliensis were observed by microscopic examination of a urine sample. a mold identified as mucor circinelloides was isolated from the specimen. it was converted to the yeast form by cultivation on sabouraud agar incubated in a gaspak jar at 37 degrees c. the isolate was eventually shown not to be related to the patient's illness; however, the superficial resemblance of the yeast phase to p. brasiliensi ... | 1987 | 3574440 |
| [infection by paracoccidioides brasiliensis in the panamanian population]. | 1987 | 3589007 | |
| resistance to paracoccidioides brasiliensis in mice is controlled by a single dominant autosomal gene. | in a previous report it was shown that there are resistant, susceptible, and intermediate strains of mice to intraperitoneal paracoccidioides brasiliensis infection. in the present work, we investigated the type of inheritance and the number of genes that determine resistance to paracoccidioidomycosis. parental and hybrid mice were inoculated intraperitoneally with 5 x 10(6) p. brasiliensis yeast cells, and mortality was scored daily. analysis of susceptible and resistant parental strains and of ... | 1987 | 3610318 |
| mycelial- to yeast-phase transitions of the dimorphic fungi blastomyces dermatitidis and paracoccidioides brasiliensis. | the physiological changes that occur during the mycelial- to yeast-phase transitions induced by a temperature shift from 25 to 37 degrees c of cultures of blastomyces dermatitidis and paracoccidioides brasiliensis can be divided into three stages. the triggering event is a heat-related insult induced by the temperature shift which results in partial uncoupling of oxidative phosphorylation and declines in cellular atp levels, respiration rates, and concentrations of electron transport components ... | 1987 | 3624202 |
| growth curves, morphology and ultrastructure of ten paracoccidioides brasiliensis isolates. | the yeast phase of ten p. brasiliensis isolates were studied to characterize their growth pattern, morphology and ultrastructure. growth curves were determined after counts of total and viable fungi units (fu) during 20 days. three growth patterns were observed: slow, reaching approximately 10-30 x 10(6) fu/tube (pb 18, pb 265 and pb 2); intermediate, reaching 60-150 x 10(6) fu/tube (ivic pb 9, ivic pb 267, pb sn, pb vitor and pb campo grande) and fast, reaching 180-370 x 10(6) fu/tube (pb 2052 ... | 1987 | 3657902 |
| experimental paracoccidioidomycosis in high and low antibody-producer mice. i.--evolution of the disease, its correlation with the humoral immune response and the patterns of tissue lesions. | high (h) and low (l) responder mice selected for their capacity to produce antibody against flagellar antigens of salmonellae sp. were infected i.p. with 10(6) l forms of paracoccidioides brasiliensis, strain 18. in h mice, the mortality ratio was 50%; the splenic index was high at the onset of infection (1.0), decreasing to normal levels after 30 days (0.45), with antibody titres such as 4-5 log2. in l mice, the mortality was 87.5%, the splenic index was above 0.6 and the antibody titres after ... | 1986 | 3729292 |
| effect of papulacandin b on glucan synthesis in paracoccidioides brasiliensis. | papulacandin b, an inhibitor of beta-glucan synthesis in some fungi, was tested on both morphological phases of paracoccidioides brasiliensis. in vivo, the antibiotic did not affect yeast morphology or growth but it inhibited mycelial growth and yeast----mycelium transformation. increase of alpha-glucan and decrease of beta-glucan synthesis was also observed in the fungal cell wall of the mycelial form after addition of papulacandin b. synthesis in vitro of beta-glucan was partially substituted ... | 1986 | 3735029 |
| ultrastructure of spore formation in paracoccidioides brasiliensis. | chlamydospores and arthrospores are produced by the mycelial phase of paracoccidioides brasiliensis in submerged cultures at 23 degrees c. arthrospores are formed by extrusion in specialized, sporogenous hyphae. the absence of any unique morphological characteristic in chlamydospores and arthrospores discards the use of spore morphology as a taxonomic criterion. | 1986 | 3735030 |
| a technique to collect and dislodge conidia produced by paracoccidioides brasiliensis mycelial form. | collection and separation of mycelial propagules produced by p. brasiliensis was accomplished by agitation with glass beads, centrifugation and filtration through cotton wool. the mean number of conidia liberated per plate (approximately 1 000 000) and their viability (79%), lead us to think that it is now possible to undertake experimental studies with these propagules. | 1986 | 3735034 |
| morphological development of the conidia produced by paracoccidioides brasiliensis mycelial form. | p. brasiliensis conidia were studied for their ability to grow as yeasts or as molds according to temperature. using a microculture system we found that such conidia shared the ability of the parent mycelium to transform directly into multiple-budding yeast cells at 36 degrees c or to produce germ tubes and branching mycelia if kept at 22 degrees c. | 1986 | 3746586 |
| langerhans' cells in paracoccidioidomycosis. | langerhans' cells (lcs) appear to be altered in diseases that express a depressed cellular immune response. we measured the density of lcs in the skin of patients with the disseminated form of paracoccidioidomycosis. the study was performed using adenosine triphosphatase staining of epidermal sheets. sixteen patients with paracoccidioidomycosis were evaluated. they had a highly significant reduction in lcs (lcs, 323 +/- 135/mm2) when compared with the number (lcs, 689 +/- 204/mm2) found in the c ... | 1987 | 3827279 |
| correlation between cell-mediated immunity and clinical forms of paracoccidioidomycosis. | cellular immune response to specific and non-specific stimulants was investigated, both in vivo and in vitro, in 29 healthy controls and in 53 previously untreated patients with the chronic isolated organic form (ciof), the chronic mixed form (cmf) and the acute progressive form (apf) of paracoccidioidomycosis. the study included skin tests to paracoccidioides brasiliensis antigen (pbag) and phytohaemagglutinin (pha), dncb sensitization, determination of t lymphocytes and complement rosette-form ... | 1985 | 3832489 |
| liquid nitrogen cryopreservation of paracoccidioides brasiliensis in fava's netto medium. | 1985 | 3836333 | |
| imported paracoccidioidomycosis: a public health problem in non-endemic areas. | diagnosis of paracoccidioidomycosis in an immigrant who had returned to italy following a long sojourn in venezuela prompted a review of imported cases of this latin american disease in nonendemic countries. forty-two such instances of well-documented paracoccidioidomycosis were complied. among these, eleven individuals had arrived in nonendemic countries with active symptomatic infections, fifteen others were first suspected of having tuberculosis, and four each were considered to have blastomy ... | 1985 | 3842116 |
| pmn chemotactic factor produced by glass-adherent cells in the acute inflammation caused by paracoccidioides brasiliensis. | intraperitoneal inoculation of bio.a mice with p. brasiliensis induces an acute inflammatory infiltrate in which 40-50% of the cells are pmn leucocytes. previous depletion of serotonin, prostaglandin, histamine and complement does not alter the course of inflammation. complement-derived factors appear to have no active participation in the process since c5-deficient mice depleted or not by cobra venom factor (cof) show the same kind of cellular influx. on the other hand, peritoneal cells incubat ... | 1985 | 3882118 |
| [initial isolation of paracoccidioides brasiliensis in panama]. | 1985 | 3895308 | |
| the ecology of paracoccidioides brasiliensis: a puzzle still unsolved. | some aspects pertaining to the ecology of the dimorphic fungus, paracoccidioides brasiliensis, are reviewed. the available facts concerning the interactions among the only known host (man), the environment (limited to certain latin-american countries) and the parasite (with an unknown habitat), are analysed. efforts are made to detect clue circumstances which may lead to discovery of the fungus micro-niche. an analysis of p. brasiliensis mycelial form reveals that such a form has the required ca ... | 1985 | 3906945 |
| paracoccidioides brasiliensis: cell wall glucans, pathogenicity, and dimorphism. | 1985 | 3916768 | |
| a yeast-derived antigen from paracoccidioides brasiliensis useful for serologic testing. | antigens prepared from p. brasiliensis yeast cells subjected to ultrasonic treatment proved reliable in the serological diagnosis of paracoccidioidomycosis. detection of antibodies was possible in over 90% from paracoccidioidomycosis patients in tests with agar gel immunodiffusion and counterimmunoelectrophoresis. specificity was high and only histoplasmosis sera produced cross reactions, albeit at a lower frequency (10%). the new antigens compared favorably to the standard yeast culture filtrat ... | 1985 | 3922069 |
| [purification of somatic antigens of paracoccidioides brasiliensis. preliminary study]. | 1985 | 3937207 | |
| [production and standardization of paracoccidioides brasiliensis, histoplasma capsulatum and aspergillus fumigatus antigens to be used in immunodiagnosis. comparison between immunodiffusion and immunoelectrophoresis]. | soluble antigens (ag) from paracoccidioides brasiliensis, histoplasma capsulatum and aspergillus fumigatus were prepared and standardized by double immunodiffusion (did) and immunoelectroosmophoresis (ieop). no difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positive with p. brasiliensis and a. fumigatus ag and 83.3% were positive with h. capsulatum ag. the specificity of the tests was verified testing 96 sera from patients with paracoccidioi ... | 1985 | 3939250 |
| lobomycosis in western brazil: report of a clinical trial with ketoconazole. | we report a case of chronic lobomycosis, contracted by a rural resident of the westernmost brazilian state, acre, and substantiate that western amazonia is now an endemic area for loboa loboi. no changes in lesion morphology resulted from a prolonged course of oral therapy with ketoconazole administered in an attempt to prevent the progressive and destructive extension of the disease. | 1986 | 3946734 |
| morphogenesis of the mycelium-to-yeast transformation in paracoccidioides brasiliensis. | the sequential changes observed during the mycelium to yeast transformation in paracoccidioides brasiliensis were studied microscopically. the mycelial elements produced terminal and intercalary swellings which, later on, became chlamydospore-like structures. these increased in size, acquired a double contour and, finally, gave rise to multiple budding cells. transformation was asynchronous. during the observation period, multiple budding cells and chlamydospores remained attached to the parent ... | 1985 | 3992430 |
| granulomatous reaction induced by lipids isolated from paracoccidioides brasiliensis. | the possibility that the histological responses produced in paracoccidioidomycosis might be attributed to lipid constituents of cells of paracoccidioides brasiliensis was investigated. charcoal particles coated with the lipid extract were prepared and the suspension inoculated intravenously into mice. the lung inflammation was characterized by an intense mononuclear and polymorphonuclear cell infiltrate surrounding the particles from two to eight days after inoculation. fractionation of lipid ex ... | 1985 | 3992645 |
| histochemical localization of succinate dehydrogenase in the mitochondria of paracoccidioides brasiliensis. | the succinate dehydrogenase activity of paracoccidioides brasiliensis was investigated histochemically by electron microscopy. the reaction product of this enzyme was demonstrated in some membranous structures of organelles interpreted as mitochondria. this enzyme shows very active oxido-reduction in paracoccidioides brasiliensis mitochondria during, 3, 6, and and 9 days of culture. | 1985 | 4012510 |
| nutritional studies on paracoccidioides brasiliensis: the role of organic sulfur in dimorphism. | the nutritional requirements of the mycelial and yeast-like phases of the dimorphic fungus paracoccidioides brasiliensis, a human pathogen, were investigated. for all nine isolates tested, mycelial cells were prototrophic, whereas yeast-like cells required a sulfur-containing amino acid for growth. moreover, changing the source of nitrogen greatly affected the morphology of the yeast-like cells. | 1985 | 4012515 |
| paracoccidioides brasiliensis arthritis. report of a case and review of the literature. | a case of paracoccidioides brasiliensis arthritis is presented. emphasis on early diagnosis and treatment is made. natives of endemic areas for paracoccidioides could present elsewhere with dissemination of this disease. | 1985 | 4032408 |
| a paracoccidioides brasiliensis polysaccharide having granuloma-inducing, toxic and macrophage-stimulating activity. | the occurrence of a polysaccharide fraction of paracoccidioides brasiliensis cell wall with toxic, granuloma-inducing and macrophage-stimulating activities was demonstrated. after fractionation of the lipid-extracted wall with 1 m-naoh, three fractions were obtained: (1) an alkali-insoluble fraction; (2) an alkali-soluble, acid-insoluble fraction and (3) an alkali-soluble, acid-soluble fraction. when the three fractions were injected into mice only fraction (1) was able to induce chronic lung in ... | 1985 | 4045421 |
| susceptibility and resistance of inbred mice to paracoccidioides brasiliensis. | nine different inbred strains of mice inoculated intraperitoneally with yeast cells of paracoccidioides brasiliensis showed significantly varying patterns of susceptibility. the a/sn strain was found to be the most resistant, while biod2/nsn, bio.a and biod2/osn the most susceptible strains. these susceptibility differences were not dependent on the size of challenge inocula and sex of animals. all strains studied showed a mean survival time proportional to the size of inocula used. although alm ... | 1985 | 4063162 |
| in vivo and in vitro characteristics of six paracoccidioides brasiliensis strains. | the yeast-like forms of six p. brasiliensis strains were characterized and compared using in vitro (growth curve determination) and in vivo (pathogenicity to sensitive inbred mice) criteria. strains pb 18 and pb 265 which behaved similarly in vitro, showing low counts of fungi and long mean generation times, were respectively the most and the least pathogenic strains. strains pb 2052 and ivic pb 267, which grow abundantly in vitro were, respectively virulent and avirulent. strains pb sn and ivic ... | 1985 | 4088290 |
| characteristics of the conidia produced by the mycelial form of paracoccidioides brasiliensis. | the sporulation capacities of the mycelial form of paracoccidioides brasiliensis were determined. five different culture media were used and four human isolates studied. conidia were produced in three agar media, namely water-agar, glucose-salts and yeast-extract. corn meal and sabouraud dextrose agars failed to induce sporulation. various types of spores were characterized with peculiar bulging arthroconidia and single-celled, pear-shaped conidia predominating. the size of these conidia varied ... | 1985 | 4095647 |
| scanning electron microscopic studies of paracoccidioides brasiliensis in the yeast phase. | scanning electron microscopy of four different paracoccidioides brasiliensis isolates in the yeast phase revealed that mother cells generating multiple, spherically shaped buds may be firmly or tenuously associated with their progeny whereas elongated buds remain attached to the mother cell through stem-like structures and may represent early stages of hypha formation. the yeast cell surfaces were covered with a delicate network of microfibrillar components. | 1985 | 4095649 |
| electron microscopic study of lobomycosis (loboa loboi). | 1972 | 4118461 | |
| [identification of paracoccidioides brasiliensis in infected tissues]. | 1973 | 4136108 | |
| chemical and immunological properties of galactomannans obtained from histoplasma duboisii, histoplasma capsulatum, paracoccidioides brasiliensis and blasomyces dermatitidis. | 1974 | 4279334 | |
| isolation of chitin and glucan from the cell wall of the yeast form of paracoccidioides brasiliensis. | 1969 | 4305157 | |
| comparison of two culture media for primary isolation of paracoccidioides brasiliensis from sputum. | 1972 | 4344842 | |
| scanning electron microscopy of paracoccidioides brasiliensis. study with and without pre-treatment with pooled sera from patients with 'south american blastomycosis'. | 1974 | 4437584 |