Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| prostaglandin e2 inhibits paracoccidioides brasiliensis killing by human monocytes. | human monocytes lacked fungicidal activity against high virulence strain of paracoccidioides brasiliensis, even after ifn-gamma activation. however, monocytes treated with indomethacin exhibited an effective killing against this fungus, suggesting a role of prostaglandin e2 (pge2) in the inhibition process. thus, the purpose of this work was to determine whether the effect of pge2 in fungicidal activity was related with decrease on h(2)o(2) release, the metabolite involved in p. brasiliensis kil ... | 2007 | 17400014 |
| paracoccin, an n-acetyl-glucosamine-binding lectin of paracoccidioides brasiliensis, is involved in fungal growth. | paracoccin is an n-acetyl-glucosamine-binding lectin from paracoccidioides brasiliensis, which can be obtained in small amounts either from culture supernatants or yeast cell extracts. in the present work, immunoelectron microscopy with mouse anti-paracoccin igg localized the antigen to the cell wall of p. brasiliensis yeast forms. paracoccin interacted with chitin, and colocalized with beta-1,4-homopolymer of glcnac to the budding sites of p. brasiliensis yeast cell. in order to evaluate the ro ... | 2007 | 17400504 |
| the transcriptome analysis of early morphogenesis in paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process. | paracoccidioides brasiliensis is a human pathogen with a broad distribution in latin america. the fungus is thermally dimorphic with two distinct forms corresponding to completely different lifestyles. upon elevation of the temperature to that of the mammalian body, the fungus adopts a yeast-like form that is exclusively associated with its pathogenic lifestyle. we describe expressed sequence tags (ests) analysis to assess the expression profile of the mycelium to yeast transition. to identify p ... | 2007 | 17425801 |
| pro- and anti-inflammatory cytokines produced by human monocytes challenged in vitro with paracoccidioides brasiliensis. | monocytes and macrophages play a central role in innate and adaptive immune response against systemic fungal infections. imbalances in suppressor or stimulatory cytokine secretion caused by these cells may influence disease development, microorganism death, and the nature of the adaptive immune response. this study analyzed the monocyte cytokine profiles of healthy individuals challenged with high and low virulent strains of p. brasiliensis and mrna cytokine expression kinetics by reverse transc ... | 2007 | 17446681 |
| analysis of glycosylinositol phosphorylceramides expressed by the opportunistic mycopathogen aspergillus fumigatus. | acidic glycosphingolipid components were extracted from the opportunistic mycopathogen aspergillus fumigatus and identified as inositol phosphorylceramide and glycosylinositol phosphorylceramides (gipcs). using nuclear magnetic resonance sppectroscopy, mass spectrometry, and other techniques, the structures of six major components were elucidated as ins-p-cer (af-0), manp(alpha1-->3)manp(alpha1-->2)ins-p-cer (af-2), manp(alpha1-->2)manp(alpha1-->3)manp(alpha1-->2)ins-p-cer (af-3a), manp(alpha1-- ... | 2007 | 17488996 |
| the mitochondrial genome from the thermal dimorphic fungus paracoccidioides brasiliensis. | we present here the sequence of the mitochondrial dna of the pathogenic thermodimorphic fungus paracoccidioides brasiliensis, agent of an endemic disease in most south american countries. the sequenced genome has 71 334 bp and is organized as a circular molecule with two gaps of unknown size flanking the middle exon of the nad5 gene. we located genes coding for the three subunits of the atp synthase (atp6, atp8 and atp9), the apocytochrome b (cob), three subunits of the cytochrome c oxidase enzy ... | 2007 | 17492801 |
| on the unknown ecological niche of paracoccidioides brasiliensis: our hypothesis of 1989: present status and perspectives. | in 1989, conti diaz & rilla published a hypothesis concerning the as yet unknown ecological niche of paracoccidioides brasiliensis. the hypothesis proposed a highly efficient paracoccidioidal ecological strategy based on an important natural reservoir of the parasite, probably in heterothermic animals from fresh water environments. further, the hypothesis proposed, a transient and variable residence in the soil with a wide aleuriospore dispersion throughout the environment together with an eleva ... | 2007 | 17505676 |
| typing of histoplasma capsulatum strains by fatty acid profile analysis. | the performance of fatty acid profiling for strain differentiation of histoplasma capsulatum was assessed. total fatty acids were isolated from the yeast-phase cells of seven stock and two previously unreported clinical strains of h. capsulatum var. capsulatum, as well as from one unreported clinical strain and one stock strain of h. capsulatum var. duboisii, and one strain of each of three other dimorphic zoopathogenic fungal species, blastomyces dermatitidis, paracoccidioides brasiliensis and ... | 2007 | 17510264 |
| towards a molecular genetic system for the pathogenic fungus paracoccidioides brasiliensis. | we herein report the development of a molecular toolbox for the dimorphic fungus paracoccidioides brasiliensis, specifically a more efficient transformation and a gene expression system. we evaluated several parameters that influence agrobacterium tumefaciens-mediated transformation (atmt), such as co-cultivation conditions and host cell susceptibility. our results show that cellular recovery and air drying of a. tumefaciens:p. brasiliensis mixtures are essential for atmt. overall, our data indi ... | 2007 | 17512227 |
| kinetics of ifn-gamma, tnf-alpha, il-10 and il-4 production by mononuclear cells stimulated with gp43 peptides, in patients cured of paracoccidioidomycosis. | we analyzed the kinetics of cytokine production by mononuclear cells from 17 patients who had been treated for paracoccidioidomycosis, using the stimulus of gp43 peptide groups (43 kda glycoprotein of paracoccidioides brasiliensis) at 0.1 and 1 microm, gp43 (1 microg/ml) and crude paracoccidioides brasiliensis antigen (pbag; 75 microg/ml). ifn-gamma production was a maximum at 144 hours in relation to the g2 and g8 peptide groups at 1 microm and was greatest at 144 hours when stimulated by gp43 ... | 2007 | 17568881 |
| identification of a gene encoding adaptin-like protein in the paracoccidioides brasiliensis genome by random amplified polymorphic dna analysis. | paracoccidioides brasiliensis isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. during this investigation, genotypic differences were observed between two samples of p. brasiliensis strain 18 yeast phase (pb18) previously cultured many times, one taken before (pb18a) and the other after (pb18b) animal inoculation. random amplified polymorphic dna analysis using the primer opj4 distinguished pb18b from pb18a by one 308 bp dna fragment, wh ... | 2007 | 17577051 |
| performance characteristics of a polyclonal enzyme immunoassay for the quantitation of histoplasma antigen in human urine samples. | an antigen detection assay was prepared with rabbit anti- histoplasma antibodies to detect and quantitate histoplasma capsulatum antigen in urine samples. by using a 4-parameter curve fit, the assay calibration ranges from 2 to 1,000 enzyme immunoassay (eia) units. we compared results for 99 urine samples with those of a reference laboratory, half of which tested positive or equivocal by that reference laboratory. performance characteristics were further defined by studying the assay linearity, ... | 2007 | 17580268 |
| report of an imported cutaneous disseminated case of paracoccidioidomycosis. | paracoccidioidomycosis is a chronic progressive infection. it affects mainly the elderly and it is geographically limited to certain areas of latin america. in europe it is considered a rare imported infection. here we report a case of paracoccidioidomycosis that occurred in a 27-year-old ecuadorian patient living in spain initially misdiagnosed as blastomycosis. the typical multi-budding yeast cells of paracoccidioides brasiliensis were observed in grocott stained samples. this case should aler ... | 2007 | 17592892 |
| the transcriptional profile of paracoccidioides brasiliensis yeast cells is influenced by human plasma. | paracoccidioides brasiliensis causes infection through host inhalation of airborne propagules of the mycelial phase of the fungus, which reach the lungs, and then disseminate to virtually all parts of the human body. here we describe the identification of differentially expressed genes in p. brasiliensis yeast cells, by analyzing cdna populations from the fungus treated with human plasma, mimicking superficial infection sites with inflammation. our analysis identified transcripts that are differ ... | 2007 | 17608708 |
| oral paracoccidioidomycosis: a case without lung manifestations. | the aim of this article is to present a case of paracoccidioidomycosis with involvement of the oral cavity but without pulmonary manifestations. | 2007 | 17618335 |
| detection of paracoccidioides brasiliensis by pcr in biopsies from patients with paracoccidioidomycosis: correlation with the histopathological pattern. | paracoccidioidomycosis, a systemic mycosis, is rarely diagnosed in its initial phase and can remain latent for up to 40 years. although pcr is sensitive for the identification of paracoccidioides brasiliensis (pb) in different samples, no study using paraffin-embedded human tissue has been published. the size of the amplicon, the fixation method and the time of the storage may affect the reaction. recently the more sensitive primer-extension-preamplification (pep)-nested-pcr has been used for am ... | 2007 | 17633806 |
| the camp pathway is important for controlling the morphological switch to the pathogenic yeast form of paracoccidioides brasiliensis. | paracoccidioides brasiliensis is a human pathogenic fungus that switches from a saprobic mycelium to a pathogenic yeast. consistent with the morphological transition being regulated by the camp-signalling pathway, there is an increase in cellular camp levels both transiently at the onset (< 24 h) and progressively in the later stages (> 120 h) of the transition to the yeast form, and this transition can be modulated by exogenous camp. we have cloned the cyr1 gene encoding adenylate cyclase (ac) ... | 2007 | 17635191 |
| a case of laryngeal paracoccidioidomycosis masquerading as chronic obstructive lung disease. | paracoccidioidomycosis (south american blastomycosis) is a systemic infection caused by a dimorphic fungus (paracoccidioides brasiliensis). it is common in the rural areas of latin america. the majority of the reported cases come from brazil, colombia and venezuela. paracoccidioidomycosis is the most important systemic mycosis of the tropical americas and can affect any organ, causing symptomatic or asymptomatic lesions. paracoccidioidomycosis can mimic other diseases, which must be considered i ... | 2007 | 17639751 |
| ultrastructural changes in paracoccidioides brasiliensis yeast cells attenuated by gamma irradiation. | paracoccidioides brasiliensis is a thermally dimorphic fungus agent of paracoccidioidomycosis, a deep-seated systemic infection of humans with high prevalence in latin america. until now no vaccine has been reported. ionizing radiation can be used to attenuate pathogens for vaccine development and we have successfully attenuated yeast cells of p. brasiliensis by gamma irradiation. the aim of the present study was to examine at ultrastructural level the effects of gamma irradiation attenuation on ... | 2007 | 17714360 |
| analysis of the paracoccidioides brasiliensis triosephosphate isomerase suggests the potential for adhesin function. | paracoccidioides brasiliensis is an important fungal pathogen. the disease it causes, paracoccidioidomycosis (pcm), ranges from localized pulmonary infection to systemic processes that endanger the life of the patient. paracoccidioides brasiliensis adhesion to host tissues contributes to its virulence, but we know relatively little about molecules and the molecular mechanisms governing fungal adhesion to mammalian cells. triosephosphate isomerase (tpi: ec 5.3.1.1) of p. brasiliensis (pbtpi) is a ... | 2007 | 17714474 |
| dimorphism and virulence in fungi. | the signature feature of systemic dimorphic fungi - a family of six primary fungal pathogens of humans - is a temperature-induced phase transition. these fungi grow as a mold in soil at ambient temperature and convert to yeast after infectious spores are inhaled into the lungs of a mammalian host. seminal work 20 years ago established that a temperature-induced phase transition from mold to yeast is required for virulence. several yeast-phase specific genes, identified one-by-one and studied by ... | 2007 | 17719267 |
| interaction between paracoccidioides brasiliensis and pulmonary dendritic cells induces interleukin-10 production and toll-like receptor-2 expression: possible mechanisms of susceptibility. | dendritic cells (dcs) are the most effective antigen-presenting cells for inducing cell-mediated immune responses; it is thus important to investigate the role played by lung dcs in the pathogenesis of paracoccidioidomycosis (pcm) and their potential to initiate an immune response in mice susceptible (b10.a) and resistant (a/j) to pcm. initially, we observed that lung dcs from susceptible mice were more phagocytic than cells from resistant mice, and we observed that phagocytosis in the presence ... | 2007 | 17763336 |
| paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay. | paracoccidioidomycosis is a systemic fungal infection caused by paracoccidioides brasiliensis. as infectious diseases can cause dna damage, the authors aimed at analyzing dna breakage in peripheral blood cells of patients with paracoccidioidomycosis by using the comet assay. the results suggested that paracoccidioidomycosis does not cause genotoxicity. | 2007 | 17876475 |
| genotyping of paracoccidioides brasiliensis directly from paraffin embedded tissue. | based on polymorphisms of the gp43 precursor gene, genotyping of paracoccidioides brasiliensis was achieved in 6 out of 10 paraffin-embedded tissue samples by modifying a nested pcr procedure used in the diagnosis of the fungal infection. nine of the samples originated in brazil. three sequences were identical to a previously published consensus sequence identifying the p. brasiliensis isolates as members of the formerly named species 1. in contrast, two sequences revealed substitutions identica ... | 2008 | 17885941 |
| molecular detection of paracoccidioides brasiliensis in road-killed wild animals. | paracoccidioides brasiliensis infections have been little studied in wild and/or domestic animals, which may represent an important indicator of the presence of the pathogen in nature. road-killed wild animals have been used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies of paracoccidiodomycosis (pcm). the presence of p. brasiliensis infection was evaluated by nested-pcr in tissue samples collected from 19 road-killed animals; 3 ... | 2008 | 17885959 |
| analysis of memory t cells in the human paracoccidioidomycosis before and during chemotherapy treatment. | memory t cell populations in patients with paracoccidioidomycosis (pcm) were analyzed before and after chemotherapy treatment. peripheral blood mononuclear cells (pbmc) collected from patients infected by paracoccidioides brasiliensis or from non-infected individuals were stimulated in vitro with either membrane and extra-cellular antigens (mexo) or yeast cell antigen preparation (pbag) of p. brasiliensis. an increase in the level of cd4(+) memory t cells was determined in pbmc from pcm patients ... | 2007 | 17897724 |
| a surface 75-kda protein with acid phosphatase activity recognized by monoclonal antibodies that inhibit paracoccidioides brasiliensis growth. | paracoccidioides brasiliensis is a thermo-dimorphic fungus responsible for paracoccidioidomycosis (pcm), a systemic granulomatous mycosis prevalent in latin america. the fungus releases many antigens which may be transiently bound to its cell surface. some of them may show enzymatic functions essential for maintaining many cell processes and survival of the microorganism at different conditions. in this study, we report the characterization of a secreted 75kda protein from p. brasiliensis with p ... | 2007 | 17913543 |
| paracoccidioidomycosis in wild monkeys from paraná state, brazil. | the aim of this study was to evaluate the seroprevalence of paracoccidioides brasiliensis infection in wild new world monkeys (cebus sp. and alouatta caraya). a total of 93 animals (cebus sp., n = 68 and alouatta caraya, n = 25) were captured in the paraná river basin, paraná state, brazil and the serum samples were analyzed by elisa and immunodiffusion using p. brasiliensis gp43 and exoantigen as antigens, respectively. the seropositivity observed by elisa was 44.1% and 60% for cebus sp. and a. ... | 2007 | 17914662 |
| immunization with radioattenuated yeast cells of paracoccidioides brasiliensis induces a long lasting protection in balb/c mice. | paracoccidioides brasiliensis is the fungus agent of paracoccidioidomycosis, a chronic systemic disease prevalent in latin america. the aim of the present work was to evaluate the protection elicited by the immunization of balb/c mice with radioattenuated yeast cells of p. brasiliensis. the immunization promoted a long lasting protection against highly infective yeast forms of p. brasiliensis. a 99.5% decrease in cfus recovery was verified 90 days post challenge. at the same time the levels of i ... | 2007 | 17920169 |
| transcriptional profile of ras1 and ras2 and the potential role of farnesylation in the dimorphism of the human pathogen paracoccidioides brasiliensis. | paracoccidioides brasiliensis is a thermo-dimorphic fungus that causes a human systemic mycosis with high incidence in latin america. owing to their participation in the control of pathogen morphogenesis, differentiation and virulence, it was decided to characterize ras genes in p. brasiliensis. ras1 and ras2 were identified to be coding for two different proteins with high identity. the ras transcriptional pattern was investigated by reverse transcription pcr (rt-pcr) during mycelium-to-yeast ( ... | 2008 | 17927766 |
| interactions of paracoccidioides brasiliensis with host cells: recent advances. | host-fungal interactions are inherently complex and dynamic. in order to identify new microbial targets and develop more effective antifungal therapies, it is important to understand the cellular and molecular mechanisms of disease. paracoccidioidomycosis provokes a variety of clinical symptoms, and paracoccidioides brasiliensis can reach many tissues, but primarily attacks the lungs. the ability of the pathogen to interact with the host surface structures is essential to further colonization, i ... | 2008 | 17940851 |
| molecular study of archival fungal strains isolated from cases of lacaziosis (jorge lobo's disease). | lacazia loboi, the aetiological agent of lacaziosis (jorge lobo's disease), is an uncultivated anomalous fungal microbe closely related to paracoccidioides brasiliensis, both restricted latin american pathogens. early reports suggesting that l. loboi had been isolated in pure culture from cases of lacaziosis, only added more confusion to the already confusing aetiology of this disease. these strains were later identified as unusual contaminants and some of them as p. brasiliensis. recent phyloge ... | 2007 | 17944708 |
| ecological study of paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection. | paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. the present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions. | 2007 | 17953742 |
| use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice for western blot analyses of lacazia loboi antigens. | antibodies in the sera of patients with lacaziosis recognized an approximately 193-kda antigen and other lacazia loboi antigens. paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in l. loboi extracts. this study is the first to examine the humoral response to l. loboi antigens by using multiple host sera. | 2008 | 17959822 |
| case of recurrent paracoccidioidomycosis in female. 10 years after initial treatment. | this report describes a case of recurrence of chronic paracoccidioidomycosis 10 years following the initial diagnosis. a 56-year-old female was admitted to the dental clinic of the pontifical catholic university of paraná complaining of oral soreness. mulberry-like ulcerations were observed on the gingiva, right labial comissura, and vermillion of the lip. the patient reported persistent chronic cough, weight loss, appetite loss and fever. the anamnesis revealed that the patient had developed an ... | 2007 | 17978547 |
| [imported mycosis: a review of paracoccidioidomycosis]. | population migration is increasing the number of systemic mycosis cases, a condition little or not present in metropolitan france, thus often weakly documented for medical teams. paracoccidioidomycosis is a systemic mycosis geographically confined to latin america. the causative agent is paracoccidioides brasiliensis, a dimorphic fungus present at ground level. discovering that the armadillo was a new host allowed documenting the ideal conditions for this fungus development. the mortality rate i ... | 2007 | 17988812 |
| oenothein b inhibits the expression of pbfks1 transcript and induces morphological changes in paracoccidioides brasiliensis. | the fungus paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis (pcm), the most prevalent human systemic mycosis in latin america. drug toxicity and the appearance of resistant strains have created the need to search for new therapeutic approaches. plants with reputed antimicrobial properties represent a rich screening source of potential antifungal compounds. in this work, the growth of p. brasiliensis yeast cells was evaluated in the presence of oenothein b extracted ... | 2007 | 18033615 |
| transcriptome profiling of paracoccidioides brasiliensis yeast-phase cells recovered from infected mice brings new insights into fungal response upon host interaction. | paracoccidioides brasiliensis is a fungal human pathogen with a wide distribution in latin america. it causes paracoccidioidomycosis, the most widespread systemic mycosis in latin america. although gene expression in p. brasiliensis had been studied, little is known about the genome sequences expressed by this species during the infection process. to better understand the infection process, 4934 expressed sequence tags (ests) derived from a non-normalized cdna library from p. brasiliensis (isola ... | 2007 | 18048933 |
| paracoccidioides brasiliensis conidia recognize fibronectin and fibrinogen which subsequently participate in adherence to human type ii alveolar cells: involvement of a specific adhesin. | we examined the ability of paracoccidioides brasiliensis conidia to interact with fibronectin, fibrinogen and with a549 cells, in order to establish the nature of the molecules involved. conidia bound to immobilized proteins in a concentration-dependent manner. antibodies against fibronectin and fibrinogen inhibited the fungal adherence to the corresponding proteins; as did laminin and fibronectin, but not fibrinogen when added in soluble form; however, the fibrinogen fragment d interfered with ... | 2008 | 18083324 |
| interleukin-15 increases paracoccidioides brasiliensis killing by human neutrophils. | interleukin-15 is a cytokine produced by a wide range of different cell types, including macrophages, in response to lipopolysaccharide or microbial infection. this cytokine may play a crucial role in the activation of phagocytic cells against pathogens, especially during innate immune response. the effects of il-15 on human polymorphonuclear leukocyte fungicidal activity against a highly virulent paracoccidioides brasiliensis strain were investigated. pretreatment of human neutrophils from heal ... | 2008 | 18086532 |
| coccidioidomycosis and other endemic mycoses in mexico. | the endemic mycoses traditionally include coccidioidomycosis, histoplasmosis, blastomycosis and paracoccidioidomycosis. although sporotrichosis and chromomycosis are technically not included among the endemic mycoses, they are frequently diagnosed in mexico. most systemic endemic mycoses are a consequence of inhaling the fungi, while subcutaneous mycoses are acquired through the inoculation of vegetable matter or soil containing the organism. coccidioidomycosis is caused by coccidioides spp., a ... | 2007 | 18095755 |
| transcriptional response of murine macrophages upon infection with opsonized paracoccidioides brasiliensis yeast cells. | paracoccidioides brasiliensis is the etiologic agent of the paracoccidioidomycosis the most common systemic mycosis in latin america. little is known about the regulation of genes involved in the innate immune host response to p. brasiliensis. we therefore examined the kinetic profile of gene expression of peritoneal macrophage infected with p. brasiliensis. total rna from macrophages at 6, 24 and 48h was extracted, hybridized onto nylon membranes and analyzed. an increase in the transcription o ... | 2008 | 18096424 |
| characterization of paracoccidioides brasiliensis pbdfg5p, a cell-wall protein implicated in filamentous growth. | the dimorphic fungus paracoccidioides brasiliensis is the causative agent of the most frequent systemic mycosis in latin america. in humans, infection starts by inhalation of fungal propagules, which reach the pulmonary epithelium and differentiate into the yeast parasitic phase. here we describe the characterization of a dfg5p (defective for filamentous growth) homologue of p. brasiliensis, a predictable cell wall protein, first identified in saccharomyces cerevisiae. the protein, the cdna and ... | 2008 | 18098122 |
| [not available]. | 1949 | 18126731 | |
| some biological characteristics of the pathogenic fungi named paracoccidioides brasiliensis and paracoccidioides cerebriformis. | 1949 | 18151038 | |
| use of recombinant gp43 isoforms expressed in pichia pastoris for diagnosis of paracoccidioidomycosis. | gp43 is the main diagnostic antigen for paracoccidioidomycosis (pcm). in vitro, gp43 expression in supernatant fluids of paracoccidioides brasiliensis cultures can be unstable, and its regulation is poorly understood. we have been able to express soluble recombinant gp43 (gp43r) isoforms as n-mannosylated proteins secreted in the supernatants of pichia pastoris cultures induced with methanol. they were secreted as major components from day 2 of induction and could be purified with affinity colum ... | 2008 | 18235042 |
| oral exfoliative cytology as a rapid diagnostic tool for paracoccidioidomycosis. | paracoccidioidomycosis is a common deep mycosis in south america. it is caused by the dimorphic fungus paracoccidioides brasiliensis. we report a case of a 47-year-old brazilian man with oral lesions due to paracoccidioidomycosis, which was diagnosed by exfoliative cytology without any special staining. we highlight this diagnostic tool as a simple, low-cost, painless, non-invasive and fast method for the diagnosis of paracoccidioidomycosis. | 2008 | 18254758 |
| fungal infection by paracoccidioides brasiliensis mimicking bone tumor. | paracoccidioides brasiliensis infection causes a systemic mycosis originally described in latin america but with current reports of worldwide distribution. the clinical presentation of paracoccidiodomycosis as an isolated long-bone lesion in children is quite unusual. this article describes a 10-year-old male with a lytic femoral bone lesion caused by p. brasiliensis infection that was first suspected of being of neoplasic etiology. the text also emphasizes the importance of including endemic fu ... | 2008 | 18266221 |
| paracoccidioidomycosis (south american blastomycosis) of the larynx mimicking carcinoma. | paracoccidioidomycosis is the most frequently identified deep mycosis in south america. paracoccidioides brasiliensis is a thermally dimorphic fungus that is generally acquired by the respiratory route through airborne inhalation of conidia. herein, we describe a case of a patient with paracoccidioidomycosis of the larynx mimicking carcinoma of the larynx and we review the literature regarding this unusual manifestation of this fungal infection. | 2008 | 18277125 |
| alveolar macrophages from susceptible mice are more competent than those of resistant mice to control initial paracoccidioides brasiliensis infection. | alveolar macrophages (am) are the first host cells to interact with paracoccidioides brasiliensis (pb), a primary human pathogen that causes severe pulmonary infections in latin america. to better understand innate immunity in pulmonary paracoccidioidomycosis, we decided to study the fungicidal and secretory abilities of am from resistant (a/j) and susceptible (b10.a) mice to infection. untreated, ifn-gamma and il-12 primed am from b10.a and a/j mice were challenged with p. brasiliensis yeasts a ... | 2008 | 18281437 |
| ccr5-dependent regulatory t cell migration mediates fungal survival and severe immunosuppression. | paracoccidioidomycosis, a debilitating pulmonary mycosis, is caused by the dimorphic fungus paracoccidioides brasiliensis. the infection results in the formation of granulomas containing viable yeast cells that are the fungal sources for disease reactivation. because cd4+cd25+ regulatory t cells (tregs) are in the lesions of patients with paracoccidioidomycosis, the migration of treg cells is dependent on the axis chemokine-chemokine receptors, and ccr5 ligands are produced in p. brasiliensis-in ... | 2008 | 18292527 |
| occurrence of antibodies to paracoccidioides brasiliensis in dairy cattle from mato grosso do sul, brazil. | the aim of this study was to evaluate the humoral immune response in cattle immunized with paracoccidioides brasiliensis and perform a seroepidemiological study of paracoccidioidomycosis in dairy cattle from mato grosso do sul. two animals (one steer and one heifer) were inoculated with a suspension of p. brasiliensis in freund incomplete adjuvant. blood samples were collected periodically to evaluate humoral immune response by immunodiffusion and elisa, using exoantigen and gp43 as antigens, re ... | 2008 | 18320347 |
| dimorphism, thermal tolerance, virulence and heat shock protein 70 transcription in different isolates of paracoccidioides brasiliensis. | the mycelia-to-yeast (m-y) transition, thermal tolerance and virulence profiles were evaluated for nine isolates of paracoccidioides brasiliensis, including samples from two of the three recently discovered cryptic species, as well as their relation to the partial sequence and transcription of the hsp70 gene. the isolates bt84 and t10 (from ps2 species) took more time to convert to yeast form and presented elongated yeast cells at 36 degrees c. arthroconidia production was also observed during t ... | 2008 | 18320348 |
| experimental paracoccidioidomycosis: alternative therapy with ajoene, compound from allium sativum, associated with sulfamethoxazole/trimethoprim. | ajoene has been described as an antithrombotic, anti-tumour, antifungal, antiparasitic and antibacterial agent. this study deals with the efficacy of ajoene to treat mice intratracheally infected with paracoccidioides brasiliensis. the results indicate that ajoene therapy is effective in association with antifungal drugs (sulfametoxazol/trimethoprim), showing a positive additive effect. ajoene-treated mice developed th1-type cytokine responses producing higher levels of ifn-gamma and il-12 when ... | 2008 | 18324489 |
| upregulation of glyoxylate cycle genes upon paracoccidioides brasiliensis internalization by murine macrophages and in vitro nutritional stress condition. | paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, is a facultative intracellular human pathogen that can persist within macrophage phagolysosomes, indicating that the fungus has evolved defense mechanisms in order to survive under nutritionally poor environments. the analysis of p. brasiliensis transcriptome revealed several virulence factor orthologs of other microorganisms, including the glyoxylate cycle genes. this cycle allows the utilization of two-carbon (c2) co ... | 2008 | 18324491 |
| expression and arrangement of extracellular matrix proteins in the lungs of mice infected with paracoccidioides brasiliensis conidia. | extracellular matrix (ecm) proteins are important modulators of migration, differentiation and proliferation for the various cell types present in the lungs; they influence the immune response as well as participate in the adherence of several fungi including paracoccidioides brasiliensis. the expression, deposition and arrangement of ecm proteins such as laminin, fibronectin, fibrinogen, collagen and proteoglycans in the lungs of mice infected with p. brasiliensis conidia has been evaluated in ... | 2008 | 18336528 |
| paracoccidioidomycosis. | paracoccidioidomycosis is a fungal infection that is relatively common in brazil, venezuela, colombia, ecuador, and argentina. sporadic cases can also be seen in some other countries. it is caused by a dimorphic fungus, paracoccidioides brasiliensis, which affects mainly the skin, lymph nodes, lungs, and oral, nasal, and gastrointestinal mucous membranes. depending on the specific immunity of the host, the infection can assume many forms and affect single or multiple organs, eventually becoming ... | 2008 | 18346557 |
| new paracoccidioides brasiliensis isolate reveals unexpected genomic variability in this human pathogen. | by means of genealogical concordance phylogenetic species recognition (gcpsr), we have investigated coding and non-coding regions from various genes and the its sequences of 7 new and 14 known isolates of paracoccidioides brasiliensis. such isolates grouped within the three phylogenetic groups recently reported in the genus paracoccidioides, with one single exception, i.e., pb01, a strain that has been the subject of intense molecular studies for many years. this isolate clearly separates from a ... | 2008 | 18364259 |
| pseudotumoral neuroparacoccidioidomycosis: one case report. | neuroparacoccidioidomycosis is the central nervous system involvement by paracoccidioides brasiliensis, a condition that may be underdiagnosed and has been scarcely reported. we describe a case of neuroparacoccidioidomycosis in a diabetic male with antecedent of heavy cigarette smoking and alcohol abuse. thirty years before, he lived in the amazon area and exerted rural activities in more recent years. the patient's main complaints were headache, visual deficit, hemiparesis, and weight loss. ima ... | 2008 | 18365765 |
| pulmonary paracoccidioidomycosis. | paracoccidioidomycosis (formerly known as south american blastomycosis) is produced by the thermally dimorphic fungus paracoccidioides brasiliensis. most often this mycosis runs a chronic progressive course affecting preferentially the lungs followed by the skin, mucous membranes, adrenals, and reticuloendothelial organs. acute-subacute presentations can be observed in children and immunosuppressed patients. occasionally, self-limited infections have been documented. two types of clinical presen ... | 2008 | 18366000 |
| treatment options for paracoccidioidomycosis and new strategies investigated. | paracoccidioidomycosis is the most prevalent systemic endemic mycosis in south america with most reported cases in brazil. it is a major cause of disability and death among young adult rural workers during their most productive years of life. sequels are frequent and the evolution of the disease and mortality burden are strongly influenced by the socio-economic status of the patients. although long periods of antifungal therapy (sulfamethoxazole/trimethoprim, itraconazole and amphotericin b) are ... | 2008 | 18380607 |
| involvement of extracellular matrix proteins in the course of experimental paracoccidioidomycosis. | we aimed at determining involvement of extracellular matrix proteins (ecmp) and an ecm-binding adhesin (32-kda protein) from paracoccidioides brasiliensis, in the course of experimental paracoccidioidomycosis. balb/c mice were infected with p. brasiliensis conidia previously incubated with soluble laminin, fibronectin and fibrinogen or a mab against the fungal adhesin. inflammatory response, chitin levels and cytokine production at different postinfection periods were determined. chitin was sign ... | 2008 | 18400008 |
| interaction of epithelial cell membrane rafts with paracoccidioides brasiliensis leads to fungal adhesion and src-family kinase activation. | membrane rafts are cholesterol- and sphingolipid-enriched cell membrane domains, which are ubiquitous in mammals and play an essential role in different cellular functions, including host cell-pathogen interaction. in this work, by using several approaches, we demonstrated the involvement of epithelial cell membrane rafts in adhesion process of the pathogenic fungus paracoccidioides brasiliensis. this conclusion was supported by the localization of ganglioside gm1, a membrane raft marker, at p. ... | 2008 | 18403242 |
| human neutrophils susceptibility to paracoccidioides brasiliensis: an ultrastructural and cytochemical assay. | paracoccidioidomycosis, caused by the dimorphic fungus paracoccidioides brasiliensis, is the most prevalent systemic mycosis of latin america, with brazil accounting for 80% of the reported cases. the great number of neutrophils found in p. brasiliensis granulomas demonstrates the importance of polymorphonuclear neutrophils (pmn) cells during this mycotic infection. it has been found that neutrophils from healthy human donors can ingest and kill the fungus through a typical phagocytic process. t ... | 2008 | 18404552 |
| expression of a kexin-like gene from the human pathogenic fungus paracoccidioides brasiliensis in saccharomyces cerevisiae. | kexin-like proteins are proteinases belonging to the subtilase family which are involved in the processing of pro-proteins to their active forms. in fungi, kexin-like proteins are involved in several important cellular processes, including mating and dimorphism. paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis undergoes a thermo-regulated dimorphic transition which is essential for the establishment of the infection. although the molecular mechanisms which rule this p ... | 2008 | 18415848 |
| bacterial and fungal pneumonias after lung transplantation. | the aim of this study was to evaluate the epidemiology of bacterial and fungal pneumonia in lung transplant (lt) recipients and to assess donor-to-host transmission of these microorganisms. | 2008 | 18455028 |
| the monoclonal antibody against the major diagnostic antigen of paracoccidioides brasiliensis mediates immune protection in infected balb/c mice challenged intratracheally with the fungus. | the protective role of specific antibodies against paracoccidioides brasiliensis is controversial. in the present study, we analyzed the effects of monoclonal antibodies on the major diagnostic antigen (gp43) using in vitro and in vivo p. brasiliensis infection models. the passive administration of some monoclonal antibodies (mabs) before and after intratracheal or intravenous infections led to a reduced fungal burden and decreased pulmonary inflammation. the protection mediated by mab 3e, the m ... | 2008 | 18458072 |
| [inquérito epidemiológico com paracoccidioidina e histoplasmina em área agrícola de café em ibiá, minas gerais, brasil.]. | an epidemiological survey in an endemic area for paracoccidioidomycosis in ibiá town, minas gerais state, brazil was carried out. for this, we used a standard form for each household and intradermal antigens of histoplasmin and paracoccidioidin. out of 194 individuals, 109(56%) were engaged in the survey showing reactivity of 44% and 49.5% for these antigens respectively. the reactivity to paracoccidioidin was observed in 50% of children below ten years. individuals who revealed reactivity to pa ... | 1998 | 18473521 |
| [in vitro comparison of antifungal activity of oxiconazole and econazole against yeast.]. | monoclonal antibodies (moabs) have had a major impact on many areas of biomedical research and almost since their advent have been used in the characterisation and identification of diagnostically important antigens of fungal pathogens. their main significance lies in three, often inter-related areas: a) the definition and characterisation of antigens for use in detection of antibody responses, b) their direct use in the detection of diagnostically useful antigen in body fluids c) their applicat ... | 1998 | 18473530 |
| isolation of paracoccidioides brasiliensis from the nine-banded armadillo dasypus novemcinctus, in an endemic area for paracoccidioidomycosis in colombia. | the microniche of the dimorphic fungus paracoccidiodes brasiliensisremains undefined in spite of the many attempts to isolate it from natural sources. until recently, knowledge was also scanty concerning the presence of natural infections in animals; however, in the last decade, the fungus has been repeatedly isolated from the nine-banded armadillo, dasypus novemcinctusin brazil. a study aimed at determining the presence of infected armadillos in one of the paracoccidioidomycosis endemic areas o ... | 1999 | 18473551 |
| fungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by paracoccidioides brasiliensis antigen. | multinucleated giant cells (mgc) are characteristic cells in granulomatous disorders such as paracoccidioidomycosis (pcm) and also are formed in vitro from peripheral blood mononuclear cells by several stimuli. in this study, the authors investigated in vitro formation of mgc derived from monocytes of healthy individuals, stimulated with paracoccidioides brasiliensis antigen (pbag), compared with other stimuli such as ifn-gamma and supernatant of con-a-stimulated peripheral blood mononuclear cel ... | 2008 | 18496765 |
| killing of paracoccidioides brasiliensis yeast cells by ifn-gamma and tnf-alpha activated murine peritoneal macrophages: evidence of h(2)o (2) and no effector mechanisms. | paracoccidioidomycosis is a deep mycosis, endemic in latin america, caused by paracoccidioides brasiliensis. macrophage activation by cytokines is the major effector mechanism against this fungus. this work aimed at a better understanding of the interaction between yeast cells-murine peritoneal macrophages and the cytokine signals required for the effective killing of high virulence yeast-form of p. brasiliensis. in addition, the killing effector mechanisms dependent on the generation of reactiv ... | 2008 | 18496766 |
| oxidative stress response in paracoccidioides brasiliensis: assessing catalase and cytochrome c peroxidase. | paracoccidioides brasiliensis is a dimorphic fungus that infects humans and establishes infection in the yeast form. we are interested in the mechanisms this fungus uses to evade the human immune system, and in its survival strategies within infected host cells. reactive oxygen species play an important role in host defence, but are detoxified by pathogen-derived antioxidant enzymes to prevent oxidative damage. the transcriptional and post-transcriptional regulation of p. brasiliensis catalase a ... | 2008 | 18499421 |
| genetic characterization of morphologically variant strains of paracoccidioides brasiliensis. | molecular characterization of paracoccidioides brasiliensis variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic dna analysis (rapd). on p. brasiliensis variants in the transitional phase and strains with typical morphology, rapd produced reproducible polymorphic amplification products that differentiated them. a dendrogram based on the generated rapd patterns placed the 14 p. brasiliensis strains into five groups with similarity c ... | 2008 | 18545858 |
| experimental infections with paracoccidioides brasiliensis obtained from armadillos: comparison to clinical isolates. | paracoccidioides brasiliensis causes paracoccidioidomycosis (pcm) that is one of the most prevalent systemic human mycoses in latin america. armadillos show a high incidence of pcm infection and could, therefore, be a natural reservoir for this fungus. in this study were compared the virulence profiles of isolates obtained from nine-banded armadillos (dasypus novemcinctus) (pbt1 and pbt4) and isolates from pcm patients (pb265 and bt83). pathogenicity was evaluated by fungal load and analysis of ... | 2008 | 18553016 |
| b-1 cells facilitate paracoccidioides brasiliensis infection in mice via il-10 secretion. | protective immunity in paracoccidioidomycosis is mainly mediated by cellular immunity. the role of b cells in this disease, in particular b-1 cells, is poorly understood. the aim of this study was to characterize the participation of b-1 cells in resistance or susceptibility of balb/c and balb/xid mice to p. brasiliensis (pb) pulmonary infection. balb/xid, which lacks b-1 cells, exhibited higher resistance to infection when compared with balb/c mice. however, adoptive transfer of b-1 cells to ba ... | 2008 | 18555722 |
| therapeutic administration of km+ lectin protects mice against paracoccidioides brasiliensis infection via interleukin-12 production in a toll-like receptor 2-dependent mechanism. | km(+) is a mannose-binding lectin from artocarpus integrifolia that induces interleukin (il)-12 production by macrophages and protective t helper 1 immune response against leishmania major infection. in this study, we performed experiments to evaluate the therapeutic activity of jackfruit km(+) (jfkm(+)) and its recombinant counterpart (rkm(+)) in experimental paracoccidioidomycosis. to this end, jfkm(+) or rkm(+) was administered to balb/c mice 10 days after infection with paracoccidiodes brasi ... | 2008 | 18599609 |
| lysozyme plays a dual role against the dimorphic fungus paracoccidioides brasiliensis. | in order to determine the role of lysozyme, an antimicrobial peptide belonging to the innate immune system, against the dimorphic fungus paracoccidioides brasiliensis, co-cultures of the mh-s murine alveolar macrophages cell line with p. brasiliensis conidia were done; assays to evaluate the effect of physiological and inflammatory concentrations of lysozyme directly on the fungus life cycle were also undertaken. we observed that tnf-alpha-activated macrophages significantly inhibited the conidi ... | 2008 | 18604417 |
| deficiency of il-12p40 subunit determines severe paracoccidioidomycosis in mice. | paracoccidioidomycosis, the major systemic mycosis in latin america, is caused by the thermally dimorphic fungus paracoccidioides brasiliensis. to investigate the role of interleukin (il)-12 in this disease, il-12p40-/- deficient mice (il-12p40-/-) and wild type mice (wt) were infected intravenously with viable yeast cells of p. brasiliensis 18 isolate. we found that, unlike wt mice, il-12p40-/- mice did not control fungal proliferation and dissemination and succumbed to infection by day 21 afte ... | 2008 | 18608917 |
| role of interleukin (il)-18 in experimental paracoccidioidomycosis. | interleukin (il)-18 has been regarded as a th1 type cytokine involved in many fungal and parasitic infections. since there have been no studies, as of yet, evaluating the role of this cytokine in paracoccidioidomycosis (pcm), we assessed the function of il-18 by using an experimental pcm model. our results showed that il-18 knockout (il-18 -/-) balb/c were more resistant to paracoccidioides brasiliensis than their littermate controls (wt). in fact, mortality rate was higher in wt mice and in the ... | 2008 | 18608933 |
| recognition of laminin by paracoccidioides brasiliensis conidia: a possible mechanism of adherence to human type ii alveolar cells. | this study addresses the recognition of laminin by paracoccidioides brasiliensis conidia, as well as its possible role in the adherence of conidia to a549 cells. adherence of conidia to immobilized laminin was shown to be specific, as anti-laminin antibodies, soluble laminin or the laminin-derived peptides ikvav and cdpgyigsr inhibited this interaction. rgd containing peptides and various monosaccharides had no effect on adherence, with the exception of n-acetylneuraminic acid. pre-treatment of ... | 2008 | 18608937 |
| paracoccidioides brasiliensis in a liquid-based papanicolaou test from a pregnant woman: report of a case. | with the exception of candida species, fungal organisms are rarely encountered in the papanicolaou test and, when encountered, usually represent contaminants rather then infections. we present the case of a healthy 29-year-old pregnant female, gravida 1, para 0, who presented for her first prenatal visit at 12-week gestation and had many large fungal-like elements with a distinct budding pattern of multiple narrow-based buds resembling a "ship's-wheel" identified in her routine liquid-based papa ... | 2008 | 18618723 |
| vaccination with heat shock protein 60 induces a protective immune response against experimental paracoccidioides brasiliensis pulmonary infection. | paracoccidioides brasiliensis causes a chronic granulomatous mycosis prevalent in latin america. the successful resolution of infection with this fungus is dependent on the activation of cellular immunity. we previously identified heat shock protein 60 (hsp60) as a target of the humoral response in paracoccidioidomycosis. herein we expressed the gene encoding hsp60 in escherichia coli and analyzed the immunological activity of this recombinant antigen. the immunization of balb/c mice with recomb ... | 2008 | 18625731 |
| helminth infections associated with multiple sclerosis induce regulatory b cells. | to assess the importance of b-cell control during parasite infections in multiple sclerosis (ms) patients. | 2008 | 18655096 |
| interleukin-6 treatment enhances human monocyte permissiveness for paracoccidioides brasiliensis growth by modulating cytokine production. | the effect of interleukin (il)-6 on cytokine production was evaluated in human monocyte cultures infected with the virulent strain of paracoccidioides brasiliensis (pb18). peripheral blood monocytes from healthy individuals were preincubated for 24 h with or without human recombinant il-6, and then challenged with pb18 for 4 h and 18 h. p. brasiliensis growth was assessed by viable fungi recovery from co-cultures after plating on bhi-agar. moreover, tumor necrosis factor-alpha (tnf-alpha), il-6 ... | 2009 | 18668419 |
| characterization of paracoccidioides brasiliensis cox9, cox12, and cox16 respiratory genes. | paracoccidioides brasiliensis is a thermo-dimorphic fungus that is the causative agent of paracoccidioidomyicosis (pcm), a human systemic granulomatous mycosis found in latin america. dimorphic transition from mycelium to yeast is required for establishing pathogenicity. dimorphism is marked by changes in mitochondrial physiology, including modulation of respiration rate. in this work, we present the identification of three p. brasiliensis nuclear genes pbcox9, pbcox12, and pbcox16 that code for ... | 2008 | 18672058 |
| phylogenetic analysis of prp8 intein in paracoccidioides brasiliensis species complex. | a recent species status investigation of the pathogenic fungus paracoccidioides brasiliensis suggested the existence of three cryptic species. in the present study, the sequences of the prp8 intein from p. brasiliensis isolates belonging to the three described genetic groups and two unidentified isolates were determined and analyzed in order to check their functionality and usefulness for species identification. all the isolates presented a full-length intein, although the endonuclease domain se ... | 2008 | 18672080 |
| co-existence of integumentary lesions and lung x-ray abnormalities in patients with paracoccidioidomycosis (pcm). | in paracoccidioidomycosis (pcm), the primary lung infection remains silent. in this study, attempts were done to define the primary target organ by correlating lung radiographic abnormalities with the time course of mucosal/skin lesions concurrently exhibited at diagnosis by 63 patients in whom microscopy and/or isolation of paracoccidioides brasiliensis from respiratory secretions had been positive. mucosal and skin lesions were found in 65.1% and 12.7% of the patients, respectively. odynophagi ... | 2008 | 18689616 |
| [proposal of new molecular characterization methods in phylogenetic studies and genotypings of pathogenic fungi]. | sequence information of 25s rrna gene was useful for the genotype determination in candida albicans. genotypes of 301 c. albicans strains by this single pcr method were determined, and out of them, two strains of new genotype (genotype e) which are closely related to c. dubliniensis in its intron structure were discovered. analysis of internal transcribed spacer (its) region sequence including 5.8s rrna region in three varieties of c. neoformans was found to be an useful method for genotype dete ... | 2008 | 18689962 |
| hypercalcemia in a patient with disseminated paracoccidioidomycosis: a case report. | hypercalcemia is well described in various granulomatous disorders, such as sarcoidosis, tuberculosis, berylliosis, leprosy and fungal infections. however, the association of paracoccidioides brasiliensis and hypercalcemia is rare: to the best of our knowledge, only two cases have previously been reported, and neither had a clear documentation of the etiology of the hypercalcemia. | 2008 | 18691418 |
| additive effect of p10 immunization and chemotherapy in anergic mice challenged intratracheally with virulent yeasts of paracoccidioides brasiliensis. | paracoccidioidomycosis is a systemic granulomatous disease manifested in the acute/subacute or chronic forms. the anergic cases of the acute/subacute form are most severe, leading to death threatening conditions. drug treatment is required to control the disease but the response in anergic patients is generally poor. a 15-mer peptide from the major diagnostic antigen gp43, named p10, induces a t-cd4+ helper-1 immune response in mice of different haplotypes and protects against intratracheal chal ... | 2008 | 18694844 |
| protective immunity induced by rpb27 of paracoccidioides brasiliensis. | a cdna coding for an antigenic protein (rpb27) from the pathogenic fungus paracoccidioides brasiliensis was cloned and its protective activity was determined against paracoccidioidomycosis (pcm). the cdna sequence contained an open reading frame (orf) of 660 base pairs encoding a protein of 219 amino acids with a predicted molecular weight of 25kda. the deduced amino acid sequence exhibited 100% identity to the 27kda p. brasiliensis hypothetic protein (access number aa49615). the complete coding ... | 2008 | 18722494 |
| altered expression of the costimulatory molecules cd80, cd86, cd152, pd-1 and icos on t-cells from paracoccidioidomycosis patients: lack of correlation with t-cell hyporesponsiveness. | t-cell proliferative hyporesponsiveness, a hallmark of paracoccidioidomycosis immune responses, underlies host's failure in controlling fungus spread, being reversible with antifungal treatment. the mechanisms leading to this hypoproliferation are not well known. since costimulatory molecules have been shown to profoundly regulate t-cell immune responses, we investigated the hypothesis that the determinants of the responder versus tolerant state may be the regulated expression of, or signaling b ... | 2008 | 18757242 |
| evidence for the role of calcineurin in morphogenesis and calcium homeostasis during mycelium-to-yeast dimorphism of paracoccidioides brasiliensis. | paracoccidioides brasiliensis is a dimorphic fungus that causes paracoccidioidomycosis, the most prevalent human deep mycosis in latin america. the dimorphic transition from mycelium to yeast (m-y) is triggered by a temperature shift from 25 degrees c to 37 degrees c and is critical for pathogenicity. intracellular ca(2+) levels increased in hyphae immediately after temperature-induced dimorphism. the chelation of ca(2+) with extracellular (egta) or intracellular (bapta) calcium chelators inhibi ... | 2008 | 18776037 |
| a centennial: discovery of paracoccidioides brasiliensis. | 2008 | 18777627 | |
| paracoccidioides brasiliensis: chemical and molecular tools for research on cell walls, antifungals, diagnosis, taxonomy. | paracoccidioides brasiliensis is a dimorphic fungus, a causative agent of paracoccidioidomycosis, one of the most frequent systemic mycoses that affect the rural population in latin america, only geographical region in which this fungus is to be found. in this work, we discuss matters related to (a) cell wall studies based on the cloning and analysis of genes involved in the synthesis of cell wall components, and their possible roles in virulence and dimorphism in p. brasiliensis, (b) molecular ... | 2008 | 18777628 |
| paracoccidioides brasiliensis: phylogenetic and ecological aspects. | the knowledge on the biological aspects of paracoccidioides brasiliensis has evolved greatly since the first description of the disease in 1908. from the pioneers, who were able to clearly demonstrate the fungal nature of the agent, to the recent genomic era, important advances have been achieved. p. brasiliensis is a true fungus, belonging to the ascomycetous division, although its sexual phase has not been demonstrated morphologically. a better understanding of the fundamental aspects of the a ... | 2008 | 18777629 |
| innate immunity to paracoccidioides brasiliensis infection. | innate immunity is based in pre-existing elements of the immune system that directly interact with all types of microbes leading to their destruction or growth inhibition. several elements of this early defense mechanism act in concert to control initial pathogen growth and have profound effect on the adaptative immune response that further develops. although most studies in paracoccidioidomycosis have been dedicated to understand cellular and humoral immune responses, innate immunity remains po ... | 2008 | 18777631 |
| insights into the pathobiology of paracoccidioides brasiliensis from transcriptome analysis--advances and perspectives. | paracoccidioiddes brasiliensis is a thermodimorphic fungus endemic to latin america, where it causes the most prevalent systemic mycosis, paracoccidioidomycosis (pcm). dna microarray technology has been used to identify patterns of gene expression when a microbe is confronted with conditions of interest, such as in vitro and/or ex vivo interaction with specific cells. p. brasiliensis is one organism that has benefited from this approach. even though its genome has not been sequenced yet, much ha ... | 2008 | 18777632 |
| comparison of transcription of multiple genes during mycelia transition to yeast cells of paracoccidioides brasiliensis reveals insights to fungal differentiation and pathogenesis. | the ascomycete paracoccidioides brasiliensis is a human pathogen with a broad distribution in latin america. the infection process of p. brasiliensis is initiated by aerially dispersed mycelia propagules, which differentiate into the yeast parasitic phase in human lungs. therefore, the transition to yeast is an initial and fundamental step in the infective process. in order to identify and characterize genes involved in p. brasiliensis transition to yeast, which could be potentially associated t ... | 2008 | 18777633 |