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transformation of the entomopathogenic fungus paecilomyces fumosoroseus with agrobacterium tumefaciens.to test the suitability of the agrobacterium tumefaciens-mediated transformation (amt) method with paecilomyces fumosoroseus, a fungal pathogen that causes diseases in a wide range of insects including whiteflies.200616706904
[pathogenicity of hyphomycet fungi to aphids aphis gossypii glover and myzus persicae (sulzer) (hemiptera: aphididae)].the aphids aphis gossypii and myzus persicae are cosmopolitan, poliphagous and damage cultivated plants. the effects of the entomopathogenic fungi beauveria bassiana (isolate ibcb 66), metarhizium anisopliae (isolate ibcb 121), paecilomyces fumosoroseus (isolate ibcb 141) and lecanicillium (= verticillium) lecanii (isolate jab 02) on third instar nymphs of a. gossypii and m. persicae were evaluated in the laboratory at 25 degrees c, 70 +/- 10% rh and 12h photophase. the aphids were transferred t ...200617144139
development of a population-based threshold model of conidial germination for analysing the effects of physiological manipulation on the stress tolerance and infectivity of insect pathogenic fungi.entomopathogenic fungi are being used as biocontrol agents of insect pests, but their efficacy can be poor in environments where water availability is reduced. in this study, the potential to improve biocontrol by physiologically manipulating fungal inoculum was investigated. cultures of beauveria bassiana, lecanicillium muscarium, lecanicillium longisporum, metarhizium anisopliae and paecilomyces fumosoroseus were manipulated by growing them under conditions of water stress, which produced coni ...200616913922
phylogenetic and structural analyses of the mating-type loci in clavicipitaceae.entomopathogens and other econutritional fungi belonging to clavicipitaceae were phylogenetically analyzed on the basis of the 18s rrna gene and mating-type genes (mat1-1-1 and mat1-2-1). the phylogenies of the mating-type genes yielded better resolutions than that of 18s rrna gene. entomopathogens (cordyceps bassiana, cordyceps brongniartii, cordyceps militaris, cordyceps sinclairii, cordyceps takaomontana, isaria cateniannulata, isaria farinosa, isaria fumosorosea, isaria javanica, lecanicilli ...200617064371
[compatibility of two fungal biocontrol agents conidia with commercial chemical acaricides].in this paper, the biological compatibility of fungal biocontrol agents beauveria bassiana and paecilomyces fumosoroseus conidia with 10 commercial chemical acaricides were assayed, based on the conidial germination rates in nutritional liquid and on sday plate. the results showed that nutritional liquid was more available than sday plate in the assay. so far as the 24 h conidial viability concerned, there were significant differences among the test acaricides with the concentrations of recommen ...200617044504
paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) as a potential mycoparasite on sphaerotheca fuliginea (ascomycotina: erysiphales).hyphomycete paecilomyces fumosoroseus that is well known as saprophytic and entomopatogenic fungus was investigated for its mycoparasitism on the cucumber powdery mildew pathogen. mycoparasitism was documented by using standard bioassay and sem. effects of mycoparasitism were evaluated in three types of experiments. paecilomyces fumosoroseus was applied in the form of graded suspensions into a colony of powdery mildew on a leaf segment. interaction between both fungi was observed as the percenta ...200515750732
susceptibility of biological stages of the horn fly, haematobia irritans, to entomopathogenic fungi (hyphomycetes).the susceptibility of the egg, pupa, and adult of haematobia irritans (l.) (diptera: muscidae) to isolates of the fungi metarhizium anisopliae (metsch.) sor., beauveria bassiana (bals.) vuill., and paecilomyces fumosoroseus (wize) brown and smith, was evaluated under laboratory conditions. groups of 20 eggs than 4 h old, pupae less than 48h old and adults were sprayed with a conidial suspension of each isolate. eggs, pupae and adults of horn fly were susceptible to these entomopathogenic fungi. ...200517119632
[beauvericin: chemical and biological aspects and occurrence].beauvericin (bea) is a cyclic hexadepsipeptide produced by beauveria bassiana, paecilomyces fumosoroseus, paecilomyces tenuipes, polyporus sulphurous, and a variety of fusarium species. this mycotoxin shows antimicrobial, insecticidal, cytotoxic, and apoptotic activity. it is the most potent specific inhibitor of cholesterol acyltransferase and possesses ionophoric properties. bea increases ion permeability in biological membranes by forming a complex with essential cations (ca2+, na+, k+), whic ...200516370518
cultivation of entomopathogenic fungi for the search of antibacterial compounds.entomopathogenic fungi are a rich source of natural bioactive compounds. to establish cultivation conditions which facilitate the production of bioactive compounds and to select good genera among entomopathogenic fungi as the producer, 47 typical entomopathogenic fungi were tested for their ability to produce antibiotic activity. thirty-eight strains (81%) and 30 strains (64%) of these fungi produced either anti-bacillus compounds or anti-staphylococcus compounds, respectively, indicating that t ...200516244901
acute oral intragastric pathogenicity and toxicity in mice of paecilomyces fumosoroseus isolated from whiteflies.paecilomyces fumosoroseus, monospore culture eh-506/3, isolated in mexico from bemisia tabaci whitefly was tested for acute oral intragastric pathogenicity and toxicity in cd-1 mice. animals were inoculated by gavage with only one dose (10(8) conidia/animal) of viable (72 mice), heat-killed (24 mice) fungus and compared to 18 control mice. clinical observations were done daily; mycological and histological tests were performed during necropsies at days 3, 10, 17, and 21 after the inoculation. no ...200516096686
impact of carbon and nitrogen nutrition on the quality, yield and composition of blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus.the impact of growing cultures of paecilomyces fumosoroseus in liquid media containing four combinations of glucose and casamino acids (8 g l(-1) or 80 g l(-1) glucose, 1.32 g l(-1) or 13.2 g l(-1) casamino acids) was evaluated, based on blastospore production, germination rate, viability after freeze-drying and short-term storage stability. when blastospores were produced using a high casamino acid concentration, blastospore yields and germination rates were significantly higher (13.2-18.5 x 10 ...200515887035
hydrophobic and electrostatic cell surface properties of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus.the physicochemical surface properties of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus were examined. contact angle measurements were performed on microbial lawns composed of blastospores of p. fumosoroseus to quantify their cell surface energy components. in addition, suspensions of the blastospores were characterized with the microbial adhesion to solvents assay. zeta potential measurements were used to quantify the surface charge and determine the zero potential of th ...200516388935
isolation of dipicolinic acid as an insecticidal toxin from paecilomyces fumosoroseus.several entomopathogenic fungi produce toxins that could be used as bioinsecticides in integrated pest management programs. paecilomyces fumosoroseus is currently used for the biological control of the whiteflies bemisia tabaci and b. argentifolii. supernatants from submerged batch culture, where the fungus produced abundant dispersed mycelium, conidia and blastospores, were toxic to the whitefly nymphs. the most abundant metabolite was purified by hplc and identified by mass spectrometry and nm ...200515696281
effect of different photoperiods on the growth, infectivity and colonization of trinidadian strains of paecilomyces fumosoroseus on the greenhouse whitefly, trialeurodes vaporariorum, using a glass slide bioassay.growth, infectivity and colonization rates for blastospores and conidia of trinidadian strains t, t10, and t11 of paecilomyces fumosoroseus (wize) brown and smith were assessed for activity against late fourth-instar nymphs of trialeurodes vaporariorum (westwood) (homoptera:aleyrodidae) under two different photoperiods (24 and 16 hour photophase). a glass-slide bioassay and a fungal development index, modified for both blastospores and conidia, were used to compare the development rates of the f ...200415861253
liquid-culture production of blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus using portable fermentation equipment.the production of fungal spores using on-site, non-sterile, portable fermentation equipment is technically constrained. very little information is available on the production requirements, such as medium concentration, inoculum stabilization, required fermentation times, and maintenance of axenic growth. in this study, we developed a two-part, liquid concentrate of the production medium that remains stable and soluble at room temperature. we also examined inoculum stability and showed that freez ...200415071762
effect of serial transfer of three strains of paecilomyces fumosoroseus on growth in vitro, virulence, and host specificity.serial passage of entomopathogenic hyphomycetes has been shown to alter virulence and host specificity. we evaluated virulence, host specificity, biomass production, conidiation, conidial germination, and a genetic fingerprint of 3 strains of paecilomyces fumosoroseus after passage in vitro or in vivo in diuraphis noxia or plutella xylostella. strain 4461 did not change in virulence toward d. noxia or p. xylostella after 30 passages in vitro nor after 15 passages in d. noxia. however, it lost vi ...200414992859
light-regulated asexual reproduction in paecilomyces fumosoroseus.the entomopathogenic fungus paecilomyces fumosoroseus has been successfully used in the control of several insect pests. asexually produced spores (conidia) are the means for dispersal and transmission of the entomopathogen; upon contact with the insect cuticle they germinate and penetrate the host. in model fungal systems it has been found that phototropism, resetting of the circadian rhythm, the induction of carotenogenesis and the development of reproductive structures are controlled by blue ...200414766909
a proposed role for the cuticular fatty amides of liposcelis bostrychophila (psocoptera: liposcelidae) in preventing adhesion of entomopathogenic fungi with dry-conidia.maximum challenge exposure of liposcelis bostrychophila to beauveria bassiana, paecilomyces fumosoroseus, aspergillus parasiticus or metarhizium anisopliae resulted in no more than 16% mortality. we investigated several of l. bostrychophila's cuticular lipids for possible contributions to its tolerance for entomopathogenic fungi. saturated c14 and c16 fatty acids did not reduce the germination rates of b. bassiana or m. anisopliae conidia. saturated c6 to c12 fatty acids that have not been ident ...200415518350
[efficacy of emulsifiable formulations of two entomopathogenic fungi against small green leafhoppers on tea plant].a field trial was conducted to test the efficacy of emulsifiable formulations of beauveria bassiana (bb) and paecilomyces fumosoroseus (pf) conidia in controlling small green leafhoppers (empoasca spp.) on tea plant in a hillside tea garden located in shuichang, zhejiang during mid-summer 2002. both bb and pf formulations contained 10(10) conidia x ml(-1). adding 3% of imidacloprid 10% wp to each fungal formulation (w/v) resulted in two other formulations, i.e., bb-imidacloprid mixture (bbim) an ...200415334957
relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of beauveria bassiana and paecilomyces fumosoroseus as fungal biocontrol agents.this study was to illustrate the relationship between the thermotolerance and the contents of hydrophobin-like or formic-acid-extractable (fae) proteins in aerial conidia of beauveria bassiana and paecilomyces fumosoroseus produced on rice-based substrate.200415239698
effects of double-stranded rna in metarhizium anisopliae var. acridum and paecilomyces fumosoroseus on protease activities, conidia production, and virulence.isogenic strains (with and without dsrna) of the entomogenous fungi metarhizium anisopliae var. acridum and paecilomyces fumosoroseus were investigated for correlation between the presence of dsrna and the production of cuticle-degrading proteases that play an important role in host parasitism, total secreted protein, and conidia production. similar levels of cuticle-degrading subtilisin-like (pr1) protease were observed for isogenic strains of m. anisopliae var. acridum after growth in medium s ...200415213741
biological control of greenhouse whitefly (trialeurodes vaporariorum) with fungal insecticides.the influence of the biological insecticide botanigard (beauveria bassiana) on different developmental stages of the greenhouse whitefly (trialeurodes vaporariorum) has been tested and compared with the influence of preferal (paecilomyces fumosoroseus), also a biological product. six experiments were set up to test the two products on eggs, which were four and seven days old, on larvae of the first, second/third and fourth stage and to test the effect on egg-deposition. these experiments were al ...200315149114
comparison of non-linear temperature-dependent development rate models applied to in vitro growth of entomopathogenic fungi.five non-linear models with three to five parameters, built to quantify the effect of temperature on insect development and microbial growth, were tested to describe the influence of temperature on in vitro-measured growth rates of entomopathogenic hyphomycetes. data from two isolates of each of the four fungal species, paecilomyces fumosoroseus, beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, were used to assess the features of each model. criteria for model evaluation incl ...200315000248
cuticular lipids and silverleaf whitefly stage affect conidial germination of beauveria bassiana and paecilomyces fumosoroseus.beauveria bassiana and paecilomyces fumosoroseus are generalist entomopathogenic fungi that infect the silverleaf whitefly (bemisia argentifolii). we found second and third instar whiteflies to be the most susceptible larval stage to both fungi. conidia of b. bassiana germinated most readily on the cuticle of second instars (54% germinated) and p. fumosoroseus germination was highest on third instar cuticle (45%). fourth instars (the ultimate instar) had low susceptibility to these pathogens, an ...200314615214
laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species.as part of an approach to select potential mycoinsecticides for aphid biocontrol, we investigated the effects of temperature on the growth, germination and pathogenicity of some hyphomycete fungi. commercially available mycoinsecticides (based on beauveria bassiana (balsamo) vuillemin and verticillium lecanii (zimmermann) viegas) and other isolates of b bassiana, v lecanii, metarhizium anisopliae (metschnikoff) sorokin and paecilomyces fumosoroseus (wize) brown & smith were evaluated. the rate o ...200312587869
[evaluation of interactive efficacy of two mycoinsecticides and low application rate imidacloprid in controlling greenhouse whitefly trialeurodes vaporariorum (homoptera: aleyrodidae)].the interactive effects of pure emulsifiable formulations (10(10) conidia.ml-1) of beauveria bassiana and paecilomyces fumosoroseus conidia and 1% and 3% of imidacloprid 10% wp were tested for controlling greenhouse whitefly, trialeurodes vaporariorum, on lettuce grown in four polyethylene film-covered greenhouses (5 m x 100 m) in kunming, yunnan. six fungal treatments, two imidacloprid ones, and one water-spray control were included in the experiment, with each being replicated in three plots ( ...200314997650
combining azadirachtin and paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) to control bemisia argentifolii (homoptera: aleyrodidae).both azadirachtin and paecilomyces fumosoroseus (wize) brown & smith have been used to control the whitefly bemisia argentifolii bellows & perring, but with only moderate effectiveness. azadirachtin is a botanical insecticide derived from the neem tree, and p. fumosoroseus is an entomopathogenic fungus. to test whether these two agents might be more effective for whitefly control if used together, different rates of each were combined in laboratory bioassays in factorial treatment. both tank mix ...200312650340
factors influencing the infectivity of isolates of paecilomyces fumosoroseus against diamondback moth, plutella xylostella.paecilomyces fumosoroseus isolate 1576 was isolated from an insect, but is avirulent against larvae of diamondback moth, plutella xylostella, and several other species. isolate 1576 grew faster and produced more conidia than isolate 4461 on potato dextrose agar. pregermination of conidia failed to increase the infectivity of isolate 1576, but the procedure did increase the infectivity of isolates 3682, 4461, and 4482. isolates 1576 and 4461 were both more infective when moisture was high during ...200111500091
penetration of cuticle and proliferation in hemolymph by paecilomyces fumosoroseus isolates that differ in virulence against lepidopteran larvae.frequencies of cuticular penetration and speed of proliferation in hemolymph were demonstrated for two isolates of paecilomyces fumosoroseus that differ in virulence against diamondback moth, plutella xylostella. penetrant hyphae of virulent isolate 4461 were visible in larval cuticle cross-sections of diamondback moth and fall armyworm, spodoptera frugiperda, within 22 h after inoculation. virtually no penetration was observed for isolate 1576 for up to 52 h after inoculation. isolate 4461 also ...200111812110
phylogeny of mitosporic entomopathogenic fungi: is the genus paecilomyces polyphyletic?we analyzed sequences of the divergent domain at the 5' end of the large subunit rrna gene from the mitosporic entomopathogenic fungi paecilomyces sp., paecilomyces fumosoroseus, paecilomyces farinosus, paecilomyces lilacinus, verticillium lecanii, verticillium psalliotae, beauveria bassiana, aschersonia sp., aschersonia placenta, ascomycetous cordyceps sp., and cordyceps militaris. phylogenetic analysis showed p. fumosorseus as the best characterized out of the analyzed species with the b. bass ...200111683463
host plant effects on activity of the mitosporic fungi beauveria bassiana and paecilomyces fumosoroseus against two populations of bemisia whiteflies (homoptera: aleyrodidae).laboratory bioassays were conducted to determine the effect of host plant on mycosis in two geographically distinct populations of early 2nd-instar nymphs of bemisia argentifolii bellows & perring from the entomopathogenic fungi beauveria bassiana (balsamo) vuillemin and paecilomyces fumosoroseus (wize) brown & smith. mycosis in b. argentifolii nymphs varied according to the host plant on which the nymphs were reared but not according to the population. both populations of whiteflies reared on c ...200111502058
effects of exogenous nutrients on conidial germination and virulence against the silverleaf whitefly for two hyphomycetes.exogenous protein and sugar sources were tested for their impact on conidial germination of two silverleaf whitefly (bemisia argentifolii) pathogens: beauveria bassiana and paecilomyces fumosoroseus. in liquid culture, sugars stimulated only 5-27% germination of b. bassiana and < or =11% germination of p. fumosoroseus, whereas, yeast extract or peptone stimulated 95-100% germination. in the absence of additional nutrients, agar alone stimulated approximately 50% germination. storing spores for d ...200111273689
pathogenicity of entomopathogenic fungi to different developmental stages of rhipicephalus sanguineus (acari: ixodidae).the pathogenicity of 4 species of entomopathogenic fungi (hyphomycetes species: beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, and paecilomyces fumosoroseus) to various developmental stages of rhipicephalus sanguineus (acari: ixodidae) ticks was compared under laboratory conditions. the most virulent isolate, m. anisopliae-108, caused 92-96% mortality to unfed larvae and nymphs on day 7 postinfection (pi) and 100% mortality to unfed adults and engorged females on day 21 pi. ...200111780821
comparison of blastospores of two paecilomyces fumosoroseus isolates: in vitro traits and virulence when injected into fall armyworm, spodoptera frugiperda.blastospores of two isolates of paecilomyces fumosoroseus were compared to determine their productivity in vitro and their relative pathogenicity after injection of fall armyworm, spodoptera frugiperda. blastospores of less virulent p. fumosoroseus isolate 1576 are smaller than those of isolate 4461, and they germinate and proliferate more slowly in vitro. the pathogenicity of injected blastospores of isolate 1576 against s. frugiperda varies with larval size. in small larvae, percentage mortali ...200111812120
genetic transformation and mutagenesis of the entomopathogenic fungus paecilomyces fumosoroseuswe have developed a dna-mediated transformation system for the entomopathogenic fungus paecilomyces fumosoroseus based on resistance to the herbicide glufosinate ammonium. the resistance is provided by the bar gene from streptomyces hygroscopicus and is under the control of the aspergillus nidulans trpc promoter and terminator sequences. frequencies of up to 110 transformants/&mgr;g of linearized plasmid dna were obtained aided by the addition of the nuclease inhibitor, aurintricarboxylic acid ( ...199910534416
influence of culture conditions on production and freeze-drying tolerance of paecilomyces fumosoroseus blastospores.with the goal of developing a defined medium for the production of desiccation-tolerant blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus, we evaluated the impact of various media components such as amino acids, carbohydrates, trace metals and vitamins on hyphal growth and sporulation of p. fumosoroseus cultures and on the freeze-drying tolerance of blastospores produced under these conditions. a comparison of 13 amino acids as sole nitrogen sources showed that glutamate, aspa ...199910510486
use of the green fluorescent protein for investigations of paecilomyces fumosoroseus in insect hosts.the green fluorescent protein (gfp) from the jellyfish aequorea victoria has been expressed in a variety of prokaryotic and eukaryotic organisms and has been used extensively as a marker in the study of host-pathogen interactions. we have expressed gfp in the entomopathogenic fungus paecilomyces fumosoroseus through co-transformation with a vector that confers resistance to glufosinate ammonium. all cell types express gfp and were readily detected by fluorescence microscopy. no correlation was o ...199910486232
pathogenicity of paecilomyces fumosoroseus isolates to diamondback moth, plutella xylostella: correlation with spore size, germination speed, and attachment to cuticle.infectivity to larvae of the diamondback moth, plutella xylostella, was compared among eight paecilomyces fumosoroseus isolates. isolate infectivity was assessed for correlation with spore length and germination speed. four isolates applied to p. xylostella cuticle were also compared for number of spores remaining on the cuticle after washing and for percentage germination after 36 h. infection of larvae inoculated with the different isolates at an average dosage of 4000 conidia/cm2 ranged from ...199910222189
germination of conidia and blastospores of paecilomyces fumosoroseus on the cuticle of the silverleaf whitefly, bemisia argentifolii. 199916308757
the effect of selected allelochemicals on germination of conidia and blastospores and mycelial growth of the entomopathogenic fungus, paecilomyces fumosoroseus (deuteromycotina: hyphomycetes).selected allelochemicals that protect plants from invasion by plant pathogenic fungi were investigated for their activity against the entomopathogenic fungus, paecilomyces fumosoroseus. the alkaloids tomatine, solanine, and camptothecin; the furanocoumarin, xanthotoxin; and the phenolic, tannic acid were tested for their effects on germination of conidia and blastospores and growth of mycelia. the lc50 values (corresponding to 50% inhibition of germination) for tomatine, solanine, camptothecin, ...199816284855
relative efficacy of blastospores and aerial conidia of paecilomyces fumosoroseus against the russian wheat aphid. 19989709022
pathogenicity of the entomopathogenic fungi paecilomyces spp. and beauveria bassiana against the silverleaf whitefly, bemisia argentifolii.pathogenicities of three species of entomopathogenic fungi against preimaginal bemisia argentifolii were measured and compared. third-instar nymphs on excised leaves of hibiscus rosa-sinensis were exposed to spray applications of 14 isolates of beauveria bassiana, 22 isolates of paecilomyces fumosoroseus, and five isolates of paecilomyces farinosus. b. bassiana and p. fumosoroseus isolates of diverse origins were highly pathogenic to the whitefly nymphs; median lethal doses of 14 of the 22 p. fu ...19989538026
preharvest mycobial population of indian jujube fruits (ziziphus mauritiana lamk.) and their implications in postharvest pathogenesis.a mycological survey was carried out on the fruit surface of indian jujube during its growth period (before harvest). a total of 32 microfungi belonging to 20 genera were isolated by surface washing technique. artificial inoculations with the isolated myco-propagules were also performed in mature jujube fruits and most of them were able to cause rot of varying severity. however, monodictys castaneae, eupenicillium lapidosum, paecilomyces fumosoroseus, trichurus spiralis and acremonium spp., alth ...19989926420
inactivation of conidia of paecilomyces fumosoroseus by near-ultraviolet (uvb and uva) and visible radiation.the detrimental effects of solar radiation, especially the ultraviolet waveband, on quiescent conidia of paecilomyces fumosoroseus were investigated. conidia were irradiated by a high-intensity source, which emitted a continuous spectrum from 270 to 1100 nm and which was equipped with long-pass filters to block short wavelengths below 280, 295, 320, or 400 nm. after irradiation, conidia were tested for germinability, survival, and infectivity toward spodoptera frugiperda larvae. it was demonstra ...19979028931
high frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus paecilomyces fumosoroseus.two different methods, (i) peg and (ii) biolistic, were employed to transform protoplasts and conidia of paecilomyces fumosoroseus using hygromycin resistance as selectable marker. transformation frequencies varied from 1.9 to 2.5 transformants microgram-1 of dna by the peg method, and from 33 to 153 transformants microgram-1 of dna by the biolistic procedure.19979368365
in vitro effects of secondary plant compounds on germination of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus (deuteromycotina: hyphomycetes)seven secondary plant compounds (catechol, chlorogenic acid, gallic acid, salicylic acid, saponin, sinigrin, and tannic acid) mixed with noble agar at three concentrations (100, 500, and 1000 ppm) were tested for their effects on germination of blastospores of the fungal entomopathogen paecilomyces fumosoroseus. with individual allelochemicals incorporated at 100 ppm in noble agar, significant differences in time to 95% germination were found between two allelochemicals (catechol and salicylic a ...19979367728
an antigenic water-soluble glucogalactomannan extracted from cell walls of paecilomyces fumosoroseus and paecilomyces farinosus.the water-soluble fraction (f1s) obtained after solubilizing in alkali the cell walls of four strains of paecilomyces fumosoroseus and two of paecilomyces farinosus amounted to 8.3-14.5% of the dry cell wall material. two polysaccharides, f1s-a (13-20%) and f1s-b (57-68%) were separated from f1s by gel permeation through sepharose cl-6b. 1h and 13c nmr spectra of f1s-b were recorded and showed analogous structural features in the six isolates of the two species. the fractions isolated from p. fu ...19969004512
genetic variability of paecilomyces fumosoroseus isolates revealed by molecular markers.paecilomyces fumosoroseus (deuteromycotina:hyphomycetes) is a fungus that is potentially useful for the bio-control of economically important agricultural pests, such as whitefly (bemisia tabaci). arbitrarily primed pcr and pcr with trna consensus primers were used to analyze genetic variability among 27 p. fumosoroseus isolates, 15 of which came from the same host, b. tabaci, one p. lilacinus isolate, used as an outgroup, 9 previously unidentified paecilomyces isolates. fifteen 10-mer oligonucl ...19957745282
beauverolides l and la from beauveria tenella and paecilomyces fumosoroseus.new beauverolides l and la were isolated and identified from the entomopathogenic fungi, beauveria tenella and paecilomyces fumosoroseus. their structures, cyclo-[3-hydroxy-4-methyldecanoyl-l-phenylalanyl-l-alanyl-d-leucyl ], and cyclo-[3-hydroxy-4-methyldecanoyl-l-phenylalanyl-l-alanyl-d-allo-i soleucyl] were deduced from hplc and gc-mass spectrometric analyses of their hydrolysates and nmr and mass spectral data.19947765754
glossina morsitans morsitans: mortalities caused in adults by experimental infection with entomopathogenic fungi.various strains of the entomopathogenic fungi: beauveria bassiana, metarhizium anisopliae, paecilomyces fumosoroseus and p. farinosus were found to be pathogenic for adult tsetse, glossina morsitans morsitans but b. bassiana and m. anisopliae were the most pathogenic, often causing mortalities of up to 100%. dose-mortality relationships were demonstrated for both b. bassiana and m. anisopliae and male tsetse were observed to be more susceptible to infection than females. pure cultures of b. bass ...19892565071
[method of biological control of triatominae, vectors of chagas disease, using entomopathogenic hyphomycetes. preliminary study].bioassays determined the pathogenic activity of 14 strains of 5 entomopathogenic hyphomycetous species (fungi imperfecti), beauveria bassiana, beauveria brongniartii, metarhizium anisopliae, nomuraea rileyi and paecilomyces fumosoroseus to rhodnius prolixus. treatments consisted of direct spraying with conidial titrated suspensions on first instar larvae. when tested at 3 x 10(5) conidia/cm2, only 2 strains, b. bassiana n. 297 and b. bassiana n. 326 killed 100% of larvae at 10 days post-exposure ...19873111731
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