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oxygen-rich culture conditions enhance the conidial infectivity and the quality of two strains of isaria fumosorosea for potentially improved biocontrol processes.in addition to high production levels of conidia, the success of entomopathogenic fungi as biological control agents depends both on their prevalence under the environmental conditions found in open fields (resistance to stress) and on the capacity of these conidia to infect pests. this study compares conidium production, infectivity and resistance to thermal and osmotic stress in two strains of isaria fumosorosea (arsef 3302 and cnrcb1) grown either under a normal atmosphere (21% o2) or using e ...201423847155
extracellular lipase of an entomopathogenic fungus effecting larvae of a scale insect.lipases play an important role in the infection process of entomopathogenic fungi by hydrolyzing the ester bonds of lipoproteins, fats and waxes present on the insect surface and in the body. here we report the purification and characterization of an extracellular lipase from isaria fumosorosea. the enzyme was purified (138.46-fold) in three steps using (nh4 )2 so4 precipitation followed by deae-cellulose and sephadex g-100 column chromatography. the molecular weight of purified enzyme was deter ...201424677050
prevalence of beauveria pseudobassiana among entomopathogenic fungi isolated from the hard tick, ixodes ricinus.human and animal disease-transmitting hard ticks (acari: ixodidae) are of great concern for public health and animal farming. alternatives to tick control by chemical acaricides are urgently needed, and one intensively evaluated biocontrol strategy is based on the use of tick-pathogenic filamentous fungi. an indispensable prerequisite for the development of tick-derived fungal isolates into registered myco-acaricides is their sound taxonomic characterisation. a set of fungal strains isolated fro ...201425065606
histopathological and molecular insights into the ovicidal activities of two entomopathogenic fungi against two-spotted spider mite.entomopathogenic fungi can infect and kill spider mite eggs but their ovicidal activities are poorly understood. here we gain histopathogenical and molecular insights into the ovicidal activities of beauveria bassiana and isaria fumosorosea against the two-spotted spider mite, tetranychus urticae. scanning electronic microscopy indicated successful adhesion and germination of fungal conidia on egg shell at 24h post-spray (hps). germ tubes of both fungi could penetrate into egg shell with penetra ...201424566192
evaluation of the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, and isaria fumosorosea for control of sitophilus oryzae.the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, and isaria fumosorosea were tested against the stored-grain pest sitophilus oryzae. the fungi were isolated from the soil (from three locations in attica, greece: b. bassiana from tatoion, m. anisopliae from marathon, and i. fumosorosea from aghios stefanos) using larvae of galleria mellonella as bait. suspensions of 2.11 × 10(7) and 2.11 × 10(8), 1.77 × 10(7) and 1.77 × 10(8), and 1.81 × 10(7) and 1.81 × 10(8) conidia per ml ...201424406003
the antifungal activity of fatty acids of all stages of sarcophaga carnaria l. (diptera: sarcophagidae).fatty acids as components of cuticular lipids of insects play a significant role in antifungal in protection against fungal infection. the chemical composition of cuticular and internal extracts obtained from all developmental stages of flesh flies sarcophaga carnaria was identified. the fatty acids were detected using gas chromatography coupled with mass spectrometry and the most abundant for all examined stages were: 18:1 > 16:0 > 16:1 > 18:0 > 18:2. polyunsaturated fatty acids (pufa) c20 were ...201423969191
optimization and synthesis of silver nanoparticles using isaria fumosorosea against human vector mosquitoes.the efficacy of silver generated larvicide with the help of entomopathogenic fungi, isaria fumosorosea (ifr) against major vector mosquitoes culex quinquefasciatus and aedes aegypti. the ifr-silver nanoparticles (agnps) were characterized structurally and functionally using uv-visible spectrophotometer followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy and fourier transform infrared (ftir) spectra. the optimum ph (alkaline), temperature (30 °c) and agitati ...201425085201
enhancing the thermotolerance of entomopathogenic isaria fumosorosea sfp-198 conidial powder by controlling the moisture content using drying and adjuvants.entomopathogenic fungi are promising pest-control agents but their industrial applicability is limited by their thermosusceptibility. with an aim to increase the thermotolerance of isaria fumosorosea sfp-198, moisture absorbents were added to dried conidial powder, and the relationship between its water potential and thermotolerance was investigated. mycotized rice grains were dried at 10℃, 20℃, 30℃, and 40℃ and the drying effect of each temperature for 24, 48, 96, and 140 hr was determined. dry ...201424808736
responsiveness of entomopathogenic fungi to menadione-induced oxidative stress.entomopathogenic fungi are predisposed to ros induced by heat and uv-a radiation when outside the insect host. when inside the host, they are subject to phagocytic cells that generate ros to eliminate invading pathogens. the oxidative stress tolerance of the entomopathogenic fungi aschersonia aleyrodis (arsef 430 and 10276), aschersonia placenta (arsef 7637), beauveria bassiana (arsef 252), isaria fumosorosea (arsef 3889), lecanicillium aphanocladii (arsef 6433), metarhizium acridum (arsef 324), ...201425457946
virulence of entomopathogenic fungi metarhizium anisopliae and paecilomyces fumosoroseus for the microbial control of spodoptera exigua.the beet armyworm spodoptera exigua (lepidoptera: noctuidae) is difficult to control using chemical insecticides because of the development of insecticide resistance. several pest control agents are used to control the beet armyworm. entomopathogenic fungi are one of the candidates for eco-friendly pest control instead of chemical control agents. in this study, among various entomopathogenic fungal strains isolated from soil two isolates were selected as high virulence pathogens against larva of ...201425606011
characterization and phylogeny of isaria spp. strains (ascomycota: hypocreales) using its1-5.8s-its2 and elongation factor 1-alpha sequences.the elongation factor 1-alpha (ef1-α) and the internal transcribed spacer (its) regions its1 and its2 (its1-5.8s-its2) sequences were used to characterize and to identify isaria isolates from argentina, mexico, and brazil, as well as to study the phylogenetic relationships among these isolates and other related fungi from the order hypocreales. the molecular characterization, which was performed by pcr-rflp of ef1-α and its1-5.8-its2 genes, was useful for resolving representative isolates of isa ...201424222441
comparison of the effect of the chosen species of saprotrophic fungi on the development of toxocara canis and ascaris suum eggs.the study aim was to compare the antagonistic interaction between saprotrophic soil fungi and embryonic development of geohelminths toxocara canis and ascaris suum. the experimental cultures were fertilized eggs of t.canis and a. suum incubated together with mycelium of strains: fusarium culmorum, metarhizium anisopliae,paecilomyces fumosoroseus, trichoderma viride and trichothecium roseum. in the control cultures the eggs of both nematode species were incubated without fungi. the experiment was ...201425281819
reduced entomopathogen abundance in myrmica ant nests-testing a possible immunological benefit of myrmecophily using galleria mellonella as a model.social insects such as ants have evolved collective rather than individual immune defence strategies against diseases and parasites at the level of their societies (colonies), known as social immunity. ants frequently host other arthropods, so-called myrmecophiles, in their nests. here, we tested the hypothesis that myrmecophily may partly arise from selection for exploiting the ants' social immunity. we used larvae of the wax moth galleria mellonella as 'model myrmecophiles' (baits) to test thi ...201526587252
evaluation of alternative plutella xylostella control by two isaria fumosorosea conidial formulations - oil-based formulation and wettable powder - combined with bacillus thuringiensis.entomopathogenic fungi are potential candidates for controlling the diamondback moth plutella xylostella (l.) (lepidoptera: plutellidae). the control efficacy of two isaria fumosorosea conidial formulations - wettable powder and oil-based formulation - combined with bacillus thuringiensis against p. xylostella was tested.201525641869
efficacy of sub lethal concentration of entomopathogenic fungi on the feeding and reproduction of spodoptera litura.in the present investigation, impact of sub lethal concentrations of entomopathogenic fungi, namely isaria fumosorosea, beauveria bassiana and paecilomyces variotii, secondary metabolite on feeding, growth, fecundity and hatchability of spodoptera litura was performed. the s. litura treated with i. fumosorosea and b. bassiana metabolites exhibited renounced food consumption. the growth rate of treated s. litura with metabolite of i. fumosorosea had drastic reduction. in the case of approximate d ...201526558183
evaluation of the time-concentration-mortality responses of plutella xylostella larvae to the interaction of isaria fumosorosea with the insecticides beta-cypermethrin and bacillus thuringiensis.entomopathogenic fungi are potential candidates for controlling plutella xylostella, a cosmopolitan pest of crucifers. in this study, bioassays were conducted to evaluate the interaction between isaria fumosorosea and sublethal doses of two insecticides, beta-cypermethrin and bacillus thuringiensis, against p. xylostella.201524668916
digestive tract mycobiota: a source of infection.the human mycobiome includes 390 fungal species detected on the skin, in the vagina, in the oral cavity, and in the digestive tract that includes 335 species and 158 genera. among these, 221 species are found only in the digestive tract, 88 only in the oral cavity, and 26 in both. these species belong to 126 genera of yeast and filamentous fungi, of the ascomycota, basidiomycota, and zygomycota phyla. forty species were identified only by culture, 188 species by molecular techniques, and 19 spec ...201525684583
effects of some bioinsecticides and entomopathogenic fungi on colorado potato beetle (leptinotarsa decemlineata l.).in this study, biological activity of entomopathogenic fungi (4 strains) isolated from the colorado potato beetle and the commercial biopesticides containing entomopathogenic fungi; priority® (paecilomyces fumosoroseus), nibortem® (verticillium lecanii), nostalgist® (beauveria bassiana), bio-magic* (metarhizium anisopliae), bio-nematon* (paeciliomyces sp.) and plant extracts; nimbedicine ec* (azadiractin) were determined against leptinotarsa decemlineata under laboratory conditions. an imidaclop ...201527145587
liquid culture fermentation for rapid production of desiccation tolerant blastospores of beauveria bassiana and isaria fumosorosea strains.a major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. previous attempts in producing beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. in this study, isolates of beauveria bassiana and isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and ...201525497914
olfactory cues from pathogenic fungus affect the direction of motion of termites, coptotermes formosanus.formosan subterranean termites, coptotermes formosanus, tend to avoid pathogen odors when tested in y-tube olfactometers, but approach and groom exposed nestmates to remove pathogens from their cuticle and maintain a healthy population. to better understand their differential reaction to pathogens and their odors, the relationship between odor cues and direction of motion was examined with the fungus isaria fumosorosea k3 strain. the results indicate that nestmate odor was strongly attractive on ...201526563202
pathogenicity of isaria fumosorosea to bemisia tabaci, with some observations on the fungal infection process and host immune response.isaria fumosorosea is an important pathogen of whiteflies, and is used as a mycoinsecticide against this pest in many regions of the world. we quantified the pathogenicity of the chinese isolate if-1106 against different life stages of sweetpotato whitefly, bemisia tabaci, on cucumber plants, and describe the infection process and aspects of the host immunological response in the laboratory. the second instar was the most susceptible life stage to infection, with mortality rates at 10(7)conidia/ ...201526264671
data in support of fumosorinone, a novel ptp1b inhibitor, activates insulin signaling in insulin-resistance hepg2 cells and shows anti-diabetic effect in diabetic kkay mice.this data article contains data related to the research article entitled "fumosorinone, a novel ptp1b inhibitor, activates insulin signaling in insulin-resistance hepg2 cells and shows anti-diabetic effect in diabetic kkay mice" in the toxicology and applied pharmacology [1]. fumosorinone (fu) is a new inhibitor of protein phosphatase 1b inhibitor, which was isolated from insect pathogenic fungi isaria fumosorosea. fu was found to inhibit ptp1b activity in our previous study [2]. ptp1b is the ph ...201526217781
expression of dsrna in recombinant isaria fumosorosea strain targets the tlr7 gene in bemisia tabaci.rna interference (rnai) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsrna/sirna into target pests. isaria fumosorosea is a common fungal pathogen of the b-biotype bemisia tabaci (whitefly), which is a widespread pest. entomopathogenic fungi directly penetrate the cuticle and invade insect hemocoel. application of i. fumosorosea expressing dsrna of whitefly immunity-related gene may aid in developing rnai technology to effe ...201526198409
critical values of porosity in rice cultures of isaria fumosorosea by adding water hyacinth: effect on conidial yields and quality.conidia of the entomopathogenic fungus isaria fumosorosea are used to control insect pests in crops. commercially available mycoinsecticides manufactured with this fungus are produced on a large scale via solid-state cultures (ssc). in order to favour gaseous exchange in scc, texturizers can be added to increase porosity fraction (ε). this work presents results of water hyacinth (eichhornia crassipes) as a novel texturizer. a mixture of parboiled rice (pr), with a ε = 0.23, was used as a substra ...201526184013
potent anti-calmodulin activity of cyclotetradepsipeptides isolated from isaria fumosorosea using a newly designed biosensor.seven cyclotetradepsipeptides, namely beauverolides c (1), f (2), i (3), ja (4), l (5), m (6), and n (7), were isolated from the entomopathogenic fungus isaria fumosorosea. the beauverolides were evaluated as potential calmodulin (cam) inhibitors using the newly designed cam biosensor hcam m124c-af350; these peptides displayed high affinity to the protein with dissociation constants (kd) ranging from 0.078 μm to 3.44 μm. beauverolide ja, the only one containing a tryptophan residue in its struct ...201525920232
structure and biosynthesis of fumosorinone, a new protein tyrosine phosphatase 1b inhibitor firstly isolated from the entomogenous fungus isaria fumosorosea.fumosorinone, isolated from the entomogenous fungus isaria fumosorosea, is a new 2-pyridone alkaloid which is elucidated by hresims 1d and 2dnmr. fumosorinone is structurally similar to tenellin and desmethylbassianin but it differs in chain length and degree of methylation. it is characterized by a classic noncompetitive inhibitor of protein tyrosine phosphatase 1b (ic50 14.04μm) which was implicated as a negative regulator of insulin receptor signaling and a potential drug target for the treat ...201525857260
fumosorinone, a novel ptp1b inhibitor, activates insulin signaling in insulin-resistance hepg2 cells and shows anti-diabetic effect in diabetic kkay mice.insulin resistance is a characteristic feature of type 2 diabetes mellitus (t2dm) and is characterized by defects in insulin signaling. protein tyrosine phosphatase 1b (ptp1b) is a key negative regulator of the insulin signaling pathways, and its increased activity and expression are implicated in the pathogenesis of insulin resistance. therefore, the inhibition of ptp1b is anticipated to become a potential therapeutic strategy to treat t2dm. fumosorinone (fu), a new natural product isolated fro ...201525796170
harvesting of chlorella sorokiniana by co-culture with the filamentous fungus isaria fumosorosea: a potential sustainable feedstock for hydrothermal gasification.despite recent advances in down-stream processing, production of microalgae remains substantially limited because of economical reasons. harvesting and dewatering are the most energy-intensive processing steps in their production and contribute 20-30% of total operational cost. bio-flocculation of microalgae by co-cultivation with filamentous fungi relies on the development of large structures that facilitate cost effective harvesting. a yet unknown filamentous fungus was isolated as a contamina ...201525795450
efficacy of entomopathogenic fungi (ascomycetes: hypocreales) against adult haematobia irritans (diptera: muscidae) under stable conditions in the mexican dry tropics.the purpose of this paper is to evaluate the effect of five strains of metarhizium anisopliae (ma) and three strains of isaria fumosorosea (ifr) at a concentration of 1×10(8)colony-forming units/ml applied by spraying onto bovines with controlled infestation of haematobia irritans under stable conditions in the mexican dry tropics. four experiments were performed, in each of which three treatments (two fungal strains and one control) were evaluated with eight repetitions for each one, by carryin ...201525771932
laboratory evaluation of isaria fumosorosea ccm 8367 and steinernema feltiae ustinov against immature stages of the colorado potato beetle.the colorado potato beetle, leptinotarsa decemlineata, has developed resistance to most registered pesticides and has become one of the most difficult insect pests to control. development of new biopesticides targeting this pest might solve the resistance problem and contribute to sustainable crop production. laboratory experiments were conducted to assess the efficacy of isaria fumosorosea (syn. paecilomyces fumosoroseus) strain ccm 8367 against l. decemlineata when applied alone or combined wi ...201627015633
the ifchit1 chitinase gene acts as a critical virulence factor in the insect pathogenic fungus isaria fumosorosea.the filamentous fungus, isaria fumosorosea, is a promising insect biological control agent. chitinases have been implicated in targeting insect cuticle structures, with biotechnological potential in insect and fungal control. the i. fumosorosea chitinase gene, ifchit1, was isolated and determined to encode a polypeptide of 423 amino acids (46 kda, pi = 6.53), present as a single copy in the i. fumosorosea genome. a split marker transformation system was developed and used to construct an ifchit1 ...201626910039
effects of isaria fumosorosea on tylcv (tomato yellow leaf curl virus) accumulation and transmitting capacity of bemisia tabaci.tomato yellow leaf curl virus (tylcv) is transmitted by the bemisia tabaci pest middle east-asia minor 1 (meam1) in china. isaria fumosorosea is a fungal pathogen of b. tabaci. however, the effects of fungal infection on tylcv expression and transmission by meam1 are unclear. in this study, potted tomatoes containing second instar nymphs of meam1 were treated with i. fumosorosea ifb01 strain and the relationship between fungal infection in meam1 and its tylcv transmission capacity was investigat ...201627716852
recombinant fungal entomopathogen rnai target insect gene.rna interference (rnai) technology is considered as an alternative for control of pests. however, rnai has not been used in field conditions yet, since delivering exogenous ds/sirna to target pests is very difficult. the laboratory methods of introducing the ds/sirna into insects through feeding, micro feeding / dripping and injecting cannot be used in fields. transgenic crop is perhaps the most effective application of rnai for pest control, but it needs long-time basic researches in order to r ...201627715447
potential of entomopathogenic fungi as biological control agents of diamondback moth (lepidoptera: plutellidae) and compatibility with chemical insecticides.the objectives were to evaluate the efficiency of entomopathogenic fungi against plutella xylostella (l.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. pathogenicity tests used isolates of beauveria bassiana, metarhizium rileyi, isaria fumosorosea, isaria sinclairii, and lecanicillium muscarium standardized at a concentration of 10(7) conidia/ml. cabbage leaf discs were immersed in these suspensions, and after evaporation o ...201626850733
further screening of entomopathogenic fungi and nematodes as control agents for drosophila suzukii.drosophila suzukii populations remain low in the uk. to date, there have been no reports of widespread damage. previous research demonstrated that various species of entomopathogenic fungi and nematodes could potentially suppress d. suzukii population development under laboratory trials. however, none of the given species was concluded to be specifically efficient in suppressing d. suzukii. therefore, there is a need to screen further species to determine their efficacy. the following entomopath ...201627294962
comparison between superficial and solid-state cultures of isaria fumosorosea: conidial yields, quality and sensitivity to oxidant conditions.conidia production and quality from mycoinsecticides in solid-state cultures (ssc) are frequently inferred from superficial culture (sc) results. both parameters were evaluated for two isaria fumosorosea strains (arsef 3302 and cnrcb1), in sc and ssc, using culture media with the same chemical composition. for both strains, conidia production was higher in sc than ssc in terms of conidia per gram of dry substrate. germination in both strains did not show significant differences between sc and ss ...201627263006
oviposition behavior and survival of tamarixia radiata (hymenoptera: eulophidae), an ectoparasitoid of the asian citrus psyllid, diaphorina citri (hemiptera: liviidae), on hosts exposed to an entomopathogenic fungus, isaria fumosorosea (hypocreales: cordycipitaceae), under laboratory conditions.antagonistic interactions between the nymphal parasitoid, tamarixia radiata waterston (hymenoptera: eulophidae), and the arsef 3581 strain of the entomopathogenic fungus, isaria fumosorosea wize (hypocreales: cordycipitaceae), could disrupt biological control of the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). three interactions were evaluated under laboratory conditions at 25 °c: 1) parasitoid survival if parasitized hosts were exposed to arsef 3581 blastospores before ...201627435930
efficacy of topical application, leaf residue or soil drench of blastospores of isaria fumosorosea for citrus root weevil management: laboratory and greenhouse investigations.the efficacy of topical, leaf residue, and soil drench applications with isaria fumosorosea blastospores (ifr strain 3581) was assessed for the management of the citrus root weevil, diaprepes abbreviatus (l.). blastospores of ifr were applied topically at a rate of 10⁷ blastospores ml(-1) on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °c, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. per ...201627879644
how to start with a clean crop: biopesticide dips reduce populations of bemisia tabaci (hemiptera: aleyrodidae) on greenhouse poinsettia propagative cuttings.(1) global movement of propagative plant material is a major pathway for introduction of bemisia tabaci (hemiptera: aleyrodidae) into poinsettia greenhouses. starting a poinsettia crop with high pest numbers disrupts otherwise successful biological control programs and widespread resistance of b. tabaci against pesticides is limiting growers' options to control this pest; (2) this study investigated the use of several biopesticides (mineral oil, insecticidal soap, beauveria bassiana, isaria fumo ...201627681741
species clarification of isaria isolates used as biocontrol agents against diaphorina citri (hemiptera: liviidae) in mexico.entomopathogenic fungi belonging to the genus isaria (hypocreales: cordycipitaceae) are promising candidates for microbial control of insect pests. currently, the mexican government is developing a biological control program based on extensive application of isaria isolates against diaphorina citri (hemiptera: liviidae), a vector of citrus huanglongbing disease. previous research identified three promising isaria isolates (che-cnrcb 303, 305, and 307; tentatively identified as isaria fumosorosea ...201626895870
improved mortality of the formosan subterranean termite by fungi, when amended with cuticle-degrading enzymes or eicosanoid biosynthesis inhibitors.formosan subterranean termites (fst) were exposed to strains of beauveria pseudobassiana (bpb) and isaria fumosorosea (ifr) to determine virulence of the fungi. once lethality was determined, sublethal doses of bpb were combined with enzymes capable of degrading the insect cuticle to measure the potential to enhance fungal infection. bpb applied to fst in combination with proteinases and a chitinase caused increased mortality over the fungus alone. mortality was enhanced when ifr was applied to ...201626122366
effect of three entomopathogenic fungi on three species of stingless bees (hymenoptera: apidae) under laboratory conditions.development of alternative strategies for pest control with reduced effect on beneficial organisms is a priority given the increasing global loss of biodiversity. biological control with entomopathogenic fungi arises as a viable option to control insect pests. however, few studies have focused on the consequences of using these organisms on pollinators other than the honey bee (apis mellifera l.) or bumble bees (bombus spp). we evaluated the pathogenicity of commercial formulations of three wide ...201627151469
comparative performance evaluation of multi-metal resistant fungal strains for simultaneous removal of multiple hazardous metals.in the present study, five fungal strains viz., aspergillus terreus aml02, paecilomyces fumosoroseus 4099, beauveria bassiana 4580, aspergillus terreus pd-17, aspergillus fumigatus pd-18, were screened for simultaneous multimetal removal. highest metal tolerance index for each individual metal viz., cd, cr, cu, ni, pb and zn (500mg/l) was recorded for a. fumigatus for the metals (cd, 0.72; cu, 0.72; pb, 1.02; zn, 0.94) followed by b. bassiana for the metals (cd, 0.56; cu, 0.14; ni, 0.29; zn, 0.8 ...201627497228
isolation and classification of fungal whitefly entomopathogens from soils of qinghai-tibet plateau and gansu corridor in china.qinghai-tibet plateau and gansu corridor of china with distinct geographic and climatic conditions are remote and less disturbed by humans, in which are likely to find some new strains of fungal entomopathogens against b-biotype whiteflies that is a very important invading pest worldwide. in this research, nineteen strains among six species of entomogenous fungi were isolated from the soil samples collected from 32 locations in qinghai-tibet plateau and gansu corridor. from the data of isolation ...201627228109
survival and immune response of the chagas vector meccus pallidipennis (hemiptera: reduviidae) against two entomopathogenic fungi, metarhizium anisopliae and isaria fumosorosea.chagas disease is a key health problem in latin america and is caused and transmitted by trypanosoma cruzi and triatomine bugs, respectively. control of triatomines has largely relied on the use pyrethroids, which has proved to be ineffective in the long term. alternatively, the use of entomopathogenic fungi has been implemented to control triatomine bugs. these fungi are highly efficient as they induce a reduction in immune response on insects. meccus pallidipennis is the main triatomine vector ...201627012246
microbial control of the invasive spiraling whitefly on cassava with entomopathogenic fungi.the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, lecanicillium lecanii and isaria fumosorosea were tested for their efficacy in managing the exotic spiraling whitefly aleurodicus dispersus (hemiptera, aleyrodidae) on cassava (manihot esculenta) during 2 seasons (2011-2012 and 2012-2013). the fungi i. fumosorosea and l. lecanii exhibited promising levels of control (> 70% mortality of the a. dispersus population). the percent mortality increased over time in both seasons. ap ...201726691465
cloning and expression analysis of the chitinase gene ifu-chit2 from isaria fumosorosea.entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. in the present study, the chitinase gene ifu-chit2 from isaria fumosorosea was investigated. the ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. the predicted ifu-chit2 protein is highly homologous to beauveria bassiana c ...201726500443
infectivity of housefly, musca domestica (diptera: muscidae) to different entomopathogenic fungi.the housefly musca domestica is a worldwide insect pest that acts as a vector for many pathogenic diseases in both people and animals. the present study was conducted to evaluate the virulence of different local isolates of beauveria bassiana, metarhizium anisopliae and isaria fumosorosea on m. domestica using two bioassay techniques: (1) adult immersion and (2) a bait method applied to both larvae and adults. the results showed evidence of a broad range of responses by both stages (larvae and a ...201727522925
lack of resistance development in bemisia tabaci to isaria fumosorosea after multiple generations of selection.the emergence of insecticide resistant insect pests is of significant concern worldwide. the whitefly, bemisia tabaci, is an important agricultural pest and has shown incredible resilience developing resistance to a number of chemical pesticides. entomopathogenic fungi such as isaria fumosorosea offer an attractive alternative to chemical pesticides for insect control, and this fungus has been shown to be an effective pathogen of b. tabaci. little is known concerning the potential for the develo ...201728230074
antiparasitic drugs: in vitro tests against nematophagous fungi.the use of biological agents has been intensified in recent years against eggs and larvae of gastrointestinal nematodes as an alternative control method in pasture plant health management, with the concomitant use with antiparasitic drugs still occurring. the aim of this study was to test the in vitro activity of the following antiparasitic drugs: ivermectin and albendazole against the following nematophagous fungi: paecilomyces fumosoroseus, paecilomyces lilacinus and paecilomyces variotii. the ...201727224732
biosafety of an entomopathogenic fungus isaria fumosorosea in an acute dermal test in rabbits.isaria fumosorosea (formerly paecilomyces fumosoroseus) is an entomopathogenic fungus that has been proposed as a low risk environmental alternative to the use of chemical insecticides to control agricultural pests and disease vectors. although there are some mycoinsecticides already being marketed in many countries, not all their biosafety protocols have been published. the acute dermal toxicity test in an animal model is one in a series of biosafety protocols that must be developed, in order t ...201727055483
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