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glossina morsitans morsitans: mortalities caused in adults by experimental infection with entomopathogenic fungi.various strains of the entomopathogenic fungi: beauveria bassiana, metarhizium anisopliae, paecilomyces fumosoroseus and p. farinosus were found to be pathogenic for adult tsetse, glossina morsitans morsitans but b. bassiana and m. anisopliae were the most pathogenic, often causing mortalities of up to 100%. dose-mortality relationships were demonstrated for both b. bassiana and m. anisopliae and male tsetse were observed to be more susceptible to infection than females. pure cultures of b. bass ...19892565071
[method of biological control of triatominae, vectors of chagas disease, using entomopathogenic hyphomycetes. preliminary study].bioassays determined the pathogenic activity of 14 strains of 5 entomopathogenic hyphomycetous species (fungi imperfecti), beauveria bassiana, beauveria brongniartii, metarhizium anisopliae, nomuraea rileyi and paecilomyces fumosoroseus to rhodnius prolixus. treatments consisted of direct spraying with conidial titrated suspensions on first instar larvae. when tested at 3 x 10(5) conidia/cm2, only 2 strains, b. bassiana n. 297 and b. bassiana n. 326 killed 100% of larvae at 10 days post-exposure ...19873111731
genetic variability of paecilomyces fumosoroseus isolates revealed by molecular markers.paecilomyces fumosoroseus (deuteromycotina:hyphomycetes) is a fungus that is potentially useful for the bio-control of economically important agricultural pests, such as whitefly (bemisia tabaci). arbitrarily primed pcr and pcr with trna consensus primers were used to analyze genetic variability among 27 p. fumosoroseus isolates, 15 of which came from the same host, b. tabaci, one p. lilacinus isolate, used as an outgroup, 9 previously unidentified paecilomyces isolates. fifteen 10-mer oligonucl ...19957745282
beauverolides l and la from beauveria tenella and paecilomyces fumosoroseus.new beauverolides l and la were isolated and identified from the entomopathogenic fungi, beauveria tenella and paecilomyces fumosoroseus. their structures, cyclo-[3-hydroxy-4-methyldecanoyl-l-phenylalanyl-l-alanyl-d-leucyl ], and cyclo-[3-hydroxy-4-methyldecanoyl-l-phenylalanyl-l-alanyl-d-allo-i soleucyl] were deduced from hplc and gc-mass spectrometric analyses of their hydrolysates and nmr and mass spectral data.19947765754
an antigenic water-soluble glucogalactomannan extracted from cell walls of paecilomyces fumosoroseus and paecilomyces farinosus.the water-soluble fraction (f1s) obtained after solubilizing in alkali the cell walls of four strains of paecilomyces fumosoroseus and two of paecilomyces farinosus amounted to 8.3-14.5% of the dry cell wall material. two polysaccharides, f1s-a (13-20%) and f1s-b (57-68%) were separated from f1s by gel permeation through sepharose cl-6b. 1h and 13c nmr spectra of f1s-b were recorded and showed analogous structural features in the six isolates of the two species. the fractions isolated from p. fu ...19969004512
inactivation of conidia of paecilomyces fumosoroseus by near-ultraviolet (uvb and uva) and visible radiation.the detrimental effects of solar radiation, especially the ultraviolet waveband, on quiescent conidia of paecilomyces fumosoroseus were investigated. conidia were irradiated by a high-intensity source, which emitted a continuous spectrum from 270 to 1100 nm and which was equipped with long-pass filters to block short wavelengths below 280, 295, 320, or 400 nm. after irradiation, conidia were tested for germinability, survival, and infectivity toward spodoptera frugiperda larvae. it was demonstra ...19979028931
in vitro effects of secondary plant compounds on germination of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus (deuteromycotina: hyphomycetes)seven secondary plant compounds (catechol, chlorogenic acid, gallic acid, salicylic acid, saponin, sinigrin, and tannic acid) mixed with noble agar at three concentrations (100, 500, and 1000 ppm) were tested for their effects on germination of blastospores of the fungal entomopathogen paecilomyces fumosoroseus. with individual allelochemicals incorporated at 100 ppm in noble agar, significant differences in time to 95% germination were found between two allelochemicals (catechol and salicylic a ...19979367728
high frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus paecilomyces fumosoroseus.two different methods, (i) peg and (ii) biolistic, were employed to transform protoplasts and conidia of paecilomyces fumosoroseus using hygromycin resistance as selectable marker. transformation frequencies varied from 1.9 to 2.5 transformants microgram-1 of dna by the peg method, and from 33 to 153 transformants microgram-1 of dna by the biolistic procedure.19979368365
pathogenicity of the entomopathogenic fungi paecilomyces spp. and beauveria bassiana against the silverleaf whitefly, bemisia argentifolii.pathogenicities of three species of entomopathogenic fungi against preimaginal bemisia argentifolii were measured and compared. third-instar nymphs on excised leaves of hibiscus rosa-sinensis were exposed to spray applications of 14 isolates of beauveria bassiana, 22 isolates of paecilomyces fumosoroseus, and five isolates of paecilomyces farinosus. b. bassiana and p. fumosoroseus isolates of diverse origins were highly pathogenic to the whitefly nymphs; median lethal doses of 14 of the 22 p. fu ...19989538026
relative efficacy of blastospores and aerial conidia of paecilomyces fumosoroseus against the russian wheat aphid. 19989709022
preharvest mycobial population of indian jujube fruits (ziziphus mauritiana lamk.) and their implications in postharvest pathogenesis.a mycological survey was carried out on the fruit surface of indian jujube during its growth period (before harvest). a total of 32 microfungi belonging to 20 genera were isolated by surface washing technique. artificial inoculations with the isolated myco-propagules were also performed in mature jujube fruits and most of them were able to cause rot of varying severity. however, monodictys castaneae, eupenicillium lapidosum, paecilomyces fumosoroseus, trichurus spiralis and acremonium spp., alth ...19989926420
pathogenicity of paecilomyces fumosoroseus isolates to diamondback moth, plutella xylostella: correlation with spore size, germination speed, and attachment to cuticle.infectivity to larvae of the diamondback moth, plutella xylostella, was compared among eight paecilomyces fumosoroseus isolates. isolate infectivity was assessed for correlation with spore length and germination speed. four isolates applied to p. xylostella cuticle were also compared for number of spores remaining on the cuticle after washing and for percentage germination after 36 h. infection of larvae inoculated with the different isolates at an average dosage of 4000 conidia/cm2 ranged from ...199910222189
use of the green fluorescent protein for investigations of paecilomyces fumosoroseus in insect hosts.the green fluorescent protein (gfp) from the jellyfish aequorea victoria has been expressed in a variety of prokaryotic and eukaryotic organisms and has been used extensively as a marker in the study of host-pathogen interactions. we have expressed gfp in the entomopathogenic fungus paecilomyces fumosoroseus through co-transformation with a vector that confers resistance to glufosinate ammonium. all cell types express gfp and were readily detected by fluorescence microscopy. no correlation was o ...199910486232
influence of culture conditions on production and freeze-drying tolerance of paecilomyces fumosoroseus blastospores.with the goal of developing a defined medium for the production of desiccation-tolerant blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus, we evaluated the impact of various media components such as amino acids, carbohydrates, trace metals and vitamins on hyphal growth and sporulation of p. fumosoroseus cultures and on the freeze-drying tolerance of blastospores produced under these conditions. a comparison of 13 amino acids as sole nitrogen sources showed that glutamate, aspa ...199910510486
genetic transformation and mutagenesis of the entomopathogenic fungus paecilomyces fumosoroseuswe have developed a dna-mediated transformation system for the entomopathogenic fungus paecilomyces fumosoroseus based on resistance to the herbicide glufosinate ammonium. the resistance is provided by the bar gene from streptomyces hygroscopicus and is under the control of the aspergillus nidulans trpc promoter and terminator sequences. frequencies of up to 110 transformants/&mgr;g of linearized plasmid dna were obtained aided by the addition of the nuclease inhibitor, aurintricarboxylic acid ( ...199910534416
effects of exogenous nutrients on conidial germination and virulence against the silverleaf whitefly for two hyphomycetes.exogenous protein and sugar sources were tested for their impact on conidial germination of two silverleaf whitefly (bemisia argentifolii) pathogens: beauveria bassiana and paecilomyces fumosoroseus. in liquid culture, sugars stimulated only 5-27% germination of b. bassiana and < or =11% germination of p. fumosoroseus, whereas, yeast extract or peptone stimulated 95-100% germination. in the absence of additional nutrients, agar alone stimulated approximately 50% germination. storing spores for d ...200111273689
factors influencing the infectivity of isolates of paecilomyces fumosoroseus against diamondback moth, plutella xylostella.paecilomyces fumosoroseus isolate 1576 was isolated from an insect, but is avirulent against larvae of diamondback moth, plutella xylostella, and several other species. isolate 1576 grew faster and produced more conidia than isolate 4461 on potato dextrose agar. pregermination of conidia failed to increase the infectivity of isolate 1576, but the procedure did increase the infectivity of isolates 3682, 4461, and 4482. isolates 1576 and 4461 were both more infective when moisture was high during ...200111500091
host plant effects on activity of the mitosporic fungi beauveria bassiana and paecilomyces fumosoroseus against two populations of bemisia whiteflies (homoptera: aleyrodidae).laboratory bioassays were conducted to determine the effect of host plant on mycosis in two geographically distinct populations of early 2nd-instar nymphs of bemisia argentifolii bellows & perring from the entomopathogenic fungi beauveria bassiana (balsamo) vuillemin and paecilomyces fumosoroseus (wize) brown & smith. mycosis in b. argentifolii nymphs varied according to the host plant on which the nymphs were reared but not according to the population. both populations of whiteflies reared on c ...200111502058
phylogeny of mitosporic entomopathogenic fungi: is the genus paecilomyces polyphyletic?we analyzed sequences of the divergent domain at the 5' end of the large subunit rrna gene from the mitosporic entomopathogenic fungi paecilomyces sp., paecilomyces fumosoroseus, paecilomyces farinosus, paecilomyces lilacinus, verticillium lecanii, verticillium psalliotae, beauveria bassiana, aschersonia sp., aschersonia placenta, ascomycetous cordyceps sp., and cordyceps militaris. phylogenetic analysis showed p. fumosorseus as the best characterized out of the analyzed species with the b. bass ...200111683463
pathogenicity of entomopathogenic fungi to different developmental stages of rhipicephalus sanguineus (acari: ixodidae).the pathogenicity of 4 species of entomopathogenic fungi (hyphomycetes species: beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, and paecilomyces fumosoroseus) to various developmental stages of rhipicephalus sanguineus (acari: ixodidae) ticks was compared under laboratory conditions. the most virulent isolate, m. anisopliae-108, caused 92-96% mortality to unfed larvae and nymphs on day 7 postinfection (pi) and 100% mortality to unfed adults and engorged females on day 21 pi. ...200111780821
penetration of cuticle and proliferation in hemolymph by paecilomyces fumosoroseus isolates that differ in virulence against lepidopteran larvae.frequencies of cuticular penetration and speed of proliferation in hemolymph were demonstrated for two isolates of paecilomyces fumosoroseus that differ in virulence against diamondback moth, plutella xylostella. penetrant hyphae of virulent isolate 4461 were visible in larval cuticle cross-sections of diamondback moth and fall armyworm, spodoptera frugiperda, within 22 h after inoculation. virtually no penetration was observed for isolate 1576 for up to 52 h after inoculation. isolate 4461 also ...200111812110
comparison of blastospores of two paecilomyces fumosoroseus isolates: in vitro traits and virulence when injected into fall armyworm, spodoptera frugiperda.blastospores of two isolates of paecilomyces fumosoroseus were compared to determine their productivity in vitro and their relative pathogenicity after injection of fall armyworm, spodoptera frugiperda. blastospores of less virulent p. fumosoroseus isolate 1576 are smaller than those of isolate 4461, and they germinate and proliferate more slowly in vitro. the pathogenicity of injected blastospores of isolate 1576 against s. frugiperda varies with larval size. in small larvae, percentage mortali ...200111812120
laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species.as part of an approach to select potential mycoinsecticides for aphid biocontrol, we investigated the effects of temperature on the growth, germination and pathogenicity of some hyphomycete fungi. commercially available mycoinsecticides (based on beauveria bassiana (balsamo) vuillemin and verticillium lecanii (zimmermann) viegas) and other isolates of b bassiana, v lecanii, metarhizium anisopliae (metschnikoff) sorokin and paecilomyces fumosoroseus (wize) brown & smith were evaluated. the rate o ...200312587869
combining azadirachtin and paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) to control bemisia argentifolii (homoptera: aleyrodidae).both azadirachtin and paecilomyces fumosoroseus (wize) brown & smith have been used to control the whitefly bemisia argentifolii bellows & perring, but with only moderate effectiveness. azadirachtin is a botanical insecticide derived from the neem tree, and p. fumosoroseus is an entomopathogenic fungus. to test whether these two agents might be more effective for whitefly control if used together, different rates of each were combined in laboratory bioassays in factorial treatment. both tank mix ...200312650340
cuticular lipids and silverleaf whitefly stage affect conidial germination of beauveria bassiana and paecilomyces fumosoroseus.beauveria bassiana and paecilomyces fumosoroseus are generalist entomopathogenic fungi that infect the silverleaf whitefly (bemisia argentifolii). we found second and third instar whiteflies to be the most susceptible larval stage to both fungi. conidia of b. bassiana germinated most readily on the cuticle of second instars (54% germinated) and p. fumosoroseus germination was highest on third instar cuticle (45%). fourth instars (the ultimate instar) had low susceptibility to these pathogens, an ...200314615214
light-regulated asexual reproduction in paecilomyces fumosoroseus.the entomopathogenic fungus paecilomyces fumosoroseus has been successfully used in the control of several insect pests. asexually produced spores (conidia) are the means for dispersal and transmission of the entomopathogen; upon contact with the insect cuticle they germinate and penetrate the host. in model fungal systems it has been found that phototropism, resetting of the circadian rhythm, the induction of carotenogenesis and the development of reproductive structures are controlled by blue ...200414766909
effect of serial transfer of three strains of paecilomyces fumosoroseus on growth in vitro, virulence, and host specificity.serial passage of entomopathogenic hyphomycetes has been shown to alter virulence and host specificity. we evaluated virulence, host specificity, biomass production, conidiation, conidial germination, and a genetic fingerprint of 3 strains of paecilomyces fumosoroseus after passage in vitro or in vivo in diuraphis noxia or plutella xylostella. strain 4461 did not change in virulence toward d. noxia or p. xylostella after 30 passages in vitro nor after 15 passages in d. noxia. however, it lost vi ...200414992859
[evaluation of interactive efficacy of two mycoinsecticides and low application rate imidacloprid in controlling greenhouse whitefly trialeurodes vaporariorum (homoptera: aleyrodidae)].the interactive effects of pure emulsifiable formulations (10(10) conidia.ml-1) of beauveria bassiana and paecilomyces fumosoroseus conidia and 1% and 3% of imidacloprid 10% wp were tested for controlling greenhouse whitefly, trialeurodes vaporariorum, on lettuce grown in four polyethylene film-covered greenhouses (5 m x 100 m) in kunming, yunnan. six fungal treatments, two imidacloprid ones, and one water-spray control were included in the experiment, with each being replicated in three plots ( ...200314997650
comparison of non-linear temperature-dependent development rate models applied to in vitro growth of entomopathogenic fungi.five non-linear models with three to five parameters, built to quantify the effect of temperature on insect development and microbial growth, were tested to describe the influence of temperature on in vitro-measured growth rates of entomopathogenic hyphomycetes. data from two isolates of each of the four fungal species, paecilomyces fumosoroseus, beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, were used to assess the features of each model. criteria for model evaluation incl ...200315000248
liquid-culture production of blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus using portable fermentation equipment.the production of fungal spores using on-site, non-sterile, portable fermentation equipment is technically constrained. very little information is available on the production requirements, such as medium concentration, inoculum stabilization, required fermentation times, and maintenance of axenic growth. in this study, we developed a two-part, liquid concentrate of the production medium that remains stable and soluble at room temperature. we also examined inoculum stability and showed that freez ...200415071762
biological control of greenhouse whitefly (trialeurodes vaporariorum) with fungal insecticides.the influence of the biological insecticide botanigard (beauveria bassiana) on different developmental stages of the greenhouse whitefly (trialeurodes vaporariorum) has been tested and compared with the influence of preferal (paecilomyces fumosoroseus), also a biological product. six experiments were set up to test the two products on eggs, which were four and seven days old, on larvae of the first, second/third and fourth stage and to test the effect on egg-deposition. these experiments were al ...200315149114
effects of double-stranded rna in metarhizium anisopliae var. acridum and paecilomyces fumosoroseus on protease activities, conidia production, and virulence.isogenic strains (with and without dsrna) of the entomogenous fungi metarhizium anisopliae var. acridum and paecilomyces fumosoroseus were investigated for correlation between the presence of dsrna and the production of cuticle-degrading proteases that play an important role in host parasitism, total secreted protein, and conidia production. similar levels of cuticle-degrading subtilisin-like (pr1) protease were observed for isogenic strains of m. anisopliae var. acridum after growth in medium s ...200415213741
relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of beauveria bassiana and paecilomyces fumosoroseus as fungal biocontrol agents.this study was to illustrate the relationship between the thermotolerance and the contents of hydrophobin-like or formic-acid-extractable (fae) proteins in aerial conidia of beauveria bassiana and paecilomyces fumosoroseus produced on rice-based substrate.200415239698
[efficacy of emulsifiable formulations of two entomopathogenic fungi against small green leafhoppers on tea plant].a field trial was conducted to test the efficacy of emulsifiable formulations of beauveria bassiana (bb) and paecilomyces fumosoroseus (pf) conidia in controlling small green leafhoppers (empoasca spp.) on tea plant in a hillside tea garden located in shuichang, zhejiang during mid-summer 2002. both bb and pf formulations contained 10(10) conidia x ml(-1). adding 3% of imidacloprid 10% wp to each fungal formulation (w/v) resulted in two other formulations, i.e., bb-imidacloprid mixture (bbim) an ...200415334957
a proposed role for the cuticular fatty amides of liposcelis bostrychophila (psocoptera: liposcelidae) in preventing adhesion of entomopathogenic fungi with dry-conidia.maximum challenge exposure of liposcelis bostrychophila to beauveria bassiana, paecilomyces fumosoroseus, aspergillus parasiticus or metarhizium anisopliae resulted in no more than 16% mortality. we investigated several of l. bostrychophila's cuticular lipids for possible contributions to its tolerance for entomopathogenic fungi. saturated c14 and c16 fatty acids did not reduce the germination rates of b. bassiana or m. anisopliae conidia. saturated c6 to c12 fatty acids that have not been ident ...200415518350
influence of temperature preferences of two paecilomyces fumosoroseus lineages on their co-infection pattern.in order to clarify the epidemiological potential of entomopathogenic fungi for insect pest control, the role of the temperature as one environmental constraint was investigated on the pattern of co-infection of galleria mellonella by two distinct lineages of a hyphomycete, paecilomyces fumosoroseus. the distribution of conidial populations collected on cadavers of hosts co-infected under 20 regimes, ranging from 13 to 35 degrees c, was examined. the apparent temperature tolerance of both fungal ...200615579318
isolation of dipicolinic acid as an insecticidal toxin from paecilomyces fumosoroseus.several entomopathogenic fungi produce toxins that could be used as bioinsecticides in integrated pest management programs. paecilomyces fumosoroseus is currently used for the biological control of the whiteflies bemisia tabaci and b. argentifolii. supernatants from submerged batch culture, where the fungus produced abundant dispersed mycelium, conidia and blastospores, were toxic to the whitefly nymphs. the most abundant metabolite was purified by hplc and identified by mass spectrometry and nm ...200515696281
paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) as a potential mycoparasite on sphaerotheca fuliginea (ascomycotina: erysiphales).hyphomycete paecilomyces fumosoroseus that is well known as saprophytic and entomopatogenic fungus was investigated for its mycoparasitism on the cucumber powdery mildew pathogen. mycoparasitism was documented by using standard bioassay and sem. effects of mycoparasitism were evaluated in three types of experiments. paecilomyces fumosoroseus was applied in the form of graded suspensions into a colony of powdery mildew on a leaf segment. interaction between both fungi was observed as the percenta ...200515750732
effect of different photoperiods on the growth, infectivity and colonization of trinidadian strains of paecilomyces fumosoroseus on the greenhouse whitefly, trialeurodes vaporariorum, using a glass slide bioassay.growth, infectivity and colonization rates for blastospores and conidia of trinidadian strains t, t10, and t11 of paecilomyces fumosoroseus (wize) brown and smith were assessed for activity against late fourth-instar nymphs of trialeurodes vaporariorum (westwood) (homoptera:aleyrodidae) under two different photoperiods (24 and 16 hour photophase). a glass-slide bioassay and a fungal development index, modified for both blastospores and conidia, were used to compare the development rates of the f ...200415861253
impact of carbon and nitrogen nutrition on the quality, yield and composition of blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus.the impact of growing cultures of paecilomyces fumosoroseus in liquid media containing four combinations of glucose and casamino acids (8 g l(-1) or 80 g l(-1) glucose, 1.32 g l(-1) or 13.2 g l(-1) casamino acids) was evaluated, based on blastospore production, germination rate, viability after freeze-drying and short-term storage stability. when blastospores were produced using a high casamino acid concentration, blastospore yields and germination rates were significantly higher (13.2-18.5 x 10 ...200515887035
acute oral intragastric pathogenicity and toxicity in mice of paecilomyces fumosoroseus isolated from whiteflies.paecilomyces fumosoroseus, monospore culture eh-506/3, isolated in mexico from bemisia tabaci whitefly was tested for acute oral intragastric pathogenicity and toxicity in cd-1 mice. animals were inoculated by gavage with only one dose (10(8) conidia/animal) of viable (72 mice), heat-killed (24 mice) fungus and compared to 18 control mice. clinical observations were done daily; mycological and histological tests were performed during necropsies at days 3, 10, 17, and 21 after the inoculation. no ...200516096686
cultivation of entomopathogenic fungi for the search of antibacterial compounds.entomopathogenic fungi are a rich source of natural bioactive compounds. to establish cultivation conditions which facilitate the production of bioactive compounds and to select good genera among entomopathogenic fungi as the producer, 47 typical entomopathogenic fungi were tested for their ability to produce antibiotic activity. thirty-eight strains (81%) and 30 strains (64%) of these fungi produced either anti-bacillus compounds or anti-staphylococcus compounds, respectively, indicating that t ...200516244901
the effect of selected allelochemicals on germination of conidia and blastospores and mycelial growth of the entomopathogenic fungus, paecilomyces fumosoroseus (deuteromycotina: hyphomycetes).selected allelochemicals that protect plants from invasion by plant pathogenic fungi were investigated for their activity against the entomopathogenic fungus, paecilomyces fumosoroseus. the alkaloids tomatine, solanine, and camptothecin; the furanocoumarin, xanthotoxin; and the phenolic, tannic acid were tested for their effects on germination of conidia and blastospores and growth of mycelia. the lc50 values (corresponding to 50% inhibition of germination) for tomatine, solanine, camptothecin, ...199816284855
germination of conidia and blastospores of paecilomyces fumosoroseus on the cuticle of the silverleaf whitefly, bemisia argentifolii. 199916308757
[beauvericin: chemical and biological aspects and occurrence].beauvericin (bea) is a cyclic hexadepsipeptide produced by beauveria bassiana, paecilomyces fumosoroseus, paecilomyces tenuipes, polyporus sulphurous, and a variety of fusarium species. this mycotoxin shows antimicrobial, insecticidal, cytotoxic, and apoptotic activity. it is the most potent specific inhibitor of cholesterol acyltransferase and possesses ionophoric properties. bea increases ion permeability in biological membranes by forming a complex with essential cations (ca2+, na+, k+), whic ...200516370518
hydrophobic and electrostatic cell surface properties of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus.the physicochemical surface properties of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus were examined. contact angle measurements were performed on microbial lawns composed of blastospores of p. fumosoroseus to quantify their cell surface energy components. in addition, suspensions of the blastospores were characterized with the microbial adhesion to solvents assay. zeta potential measurements were used to quantify the surface charge and determine the zero potential of th ...200516388935
transformation of the entomopathogenic fungus paecilomyces fumosoroseus with agrobacterium tumefaciens.to test the suitability of the agrobacterium tumefaciens-mediated transformation (amt) method with paecilomyces fumosoroseus, a fungal pathogen that causes diseases in a wide range of insects including whiteflies.200616706904
development of a population-based threshold model of conidial germination for analysing the effects of physiological manipulation on the stress tolerance and infectivity of insect pathogenic fungi.entomopathogenic fungi are being used as biocontrol agents of insect pests, but their efficacy can be poor in environments where water availability is reduced. in this study, the potential to improve biocontrol by physiologically manipulating fungal inoculum was investigated. cultures of beauveria bassiana, lecanicillium muscarium, lecanicillium longisporum, metarhizium anisopliae and paecilomyces fumosoroseus were manipulated by growing them under conditions of water stress, which produced coni ...200616913922
[compatibility of two fungal biocontrol agents conidia with commercial chemical acaricides].in this paper, the biological compatibility of fungal biocontrol agents beauveria bassiana and paecilomyces fumosoroseus conidia with 10 commercial chemical acaricides were assayed, based on the conidial germination rates in nutritional liquid and on sday plate. the results showed that nutritional liquid was more available than sday plate in the assay. so far as the 24 h conidial viability concerned, there were significant differences among the test acaricides with the concentrations of recommen ...200617044504
phylogenetic and structural analyses of the mating-type loci in clavicipitaceae.entomopathogens and other econutritional fungi belonging to clavicipitaceae were phylogenetically analyzed on the basis of the 18s rrna gene and mating-type genes (mat1-1-1 and mat1-2-1). the phylogenies of the mating-type genes yielded better resolutions than that of 18s rrna gene. entomopathogens (cordyceps bassiana, cordyceps brongniartii, cordyceps militaris, cordyceps sinclairii, cordyceps takaomontana, isaria cateniannulata, isaria farinosa, isaria fumosorosea, isaria javanica, lecanicilli ...200617064371
susceptibility of biological stages of the horn fly, haematobia irritans, to entomopathogenic fungi (hyphomycetes).the susceptibility of the egg, pupa, and adult of haematobia irritans (l.) (diptera: muscidae) to isolates of the fungi metarhizium anisopliae (metsch.) sor., beauveria bassiana (bals.) vuill., and paecilomyces fumosoroseus (wize) brown and smith, was evaluated under laboratory conditions. groups of 20 eggs than 4 h old, pupae less than 48h old and adults were sprayed with a conidial suspension of each isolate. eggs, pupae and adults of horn fly were susceptible to these entomopathogenic fungi. ...200517119632
[pathogenicity of hyphomycet fungi to aphids aphis gossypii glover and myzus persicae (sulzer) (hemiptera: aphididae)].the aphids aphis gossypii and myzus persicae are cosmopolitan, poliphagous and damage cultivated plants. the effects of the entomopathogenic fungi beauveria bassiana (isolate ibcb 66), metarhizium anisopliae (isolate ibcb 121), paecilomyces fumosoroseus (isolate ibcb 141) and lecanicillium (= verticillium) lecanii (isolate jab 02) on third instar nymphs of a. gossypii and m. persicae were evaluated in the laboratory at 25 degrees c, 70 +/- 10% rh and 12h photophase. the aphids were transferred t ...200617144139
virulence testing and extracellular subtilisin-like (pr1) and trypsin-like (pr2) activity during propagule production of paecilomyces fumosoroseus isolates from whiteflies (homoptera: aleyrodidae).to properly characterize several isolates of paecilomyces fumosoroseus, a fungal entomopathogen of whiteflies (homoptera: aleyrodidae) and other insect pests for biocontrol purposes, virulence towards trialeurodes vaporariorum, and subtilisin-like (pr1) and trypsin-like (pr2) protease activity during propagule production were investigated in monospore cultures (mcs). the virulence of three mcs towards second instar whiteflies was measured and expressed as lethal median concentration (lc50). numb ...200717592897
[pathogenicity of several fungal species on spodoptera litura].the virulence test of five species of entomogenous fungi beauveria brongniartii, beauveria bassiana, paecilomyces fumosoroseus, metarhizium anisopliae and nomuraea rileyi to spodoptera litura larvae showed that b. brongniartii and n. rileyi had evident pathogenic effects on s. litura, with the lt50 value to s. litura 2nd instars being 2.95 and 4.10 days, and the corrected accumulative mortality of the instars being 100% and 95.2%, respectively. the virulence of b. brongniartii and n. rileyi to t ...200717615897
[intraspecific variations of paecilomyces fumosoroseus in temperature fitness and carbendazim resistance].a total of six wild strains of fungal biocontrol agent paecilomyces fumosoroseus were grown at 15 degrees c-35 degrees c to reveal their variations in colony growth, conidiation, and conidial viability. the results showed that the test three indices were the best at 25 degrees c, but differed significantly at different temperatures for a given strain or at a given temperature among test strains. the strains pfr116 and pfr6206 behaved better or more stably than other strains at 15 degrees c-35 de ...200717886653
ovicidal activity of entomopathogenic hyphomycetes on aedes aegypti (diptera: culicidae) under laboratory conditions.the ovicidal activity of 21 hyphomycete fungi species against aedes aegypti (l.) (diptera: culicidae) was tested. fungi with ovicidal activity developed on high numbers of eggs (> or =70%) during 25 d of exposure. a clear ovicidal activity with low values of hatch (1.3-40%) was observed after 25 d of incubation with isaria farinosa (holm: fries) fries, paecilomyces carneus (duché & heim) brown & smith, paecilomyces marquandii (massee) hughes, isaria fumosorosea (wize), metarhizium anisopliae (me ...200717915511
defense mechanism of the termite, coptotermes formosanus shiraki, to entomopathogenic fungi.termites, coptotermes formosanus shiraki, reared individually, were highly susceptible to entomopathogenic fungi, paecilomyces fumosoroseus and beauveria brongniartii and metarhizium anisopliae, while termites reared in groups were highly resistant. quantitative assays with an epifluoresent microscope revealed a significant difference in the number of conidia attachments among three entomopathogenic fungi. the conidia of b. brongniartii and p. fumosoroseus bound to termite cuticles more effectiv ...200817949740
microsatellite variability in the entomopathogenic fungus paecilomyces fumosoroseus: genetic diversity and population structure.the hyphomycete paecilomyces fumosoroseus (pfr) is a geographically widespread fungus capable of infecting various insect hosts. the fungus has been used for the biological control of several important insect pests of agriculture. however knowledge of the fungus' genetic diversity and population structure is required for its sustainable use as a biological control agent. we investigated length and sequence polymorphisms of nine microsatellite loci for 33 pfr accessions sampled from various host ...200818268903
pathogenicity of entomopathogenic fungi on hibernating pupae of cameraria ohridella deschka & dimic 1986 (lepidoptera, gracillariidae). part 1: pathogenicity against the naked pupa.strains from paecilomyces fumosoroseus, lecanicillium muscarium, metarhizium anisopliae and beauveria bassiana were examined in standardized biotest to control the horse-chestnut leaf miner (cameraria ohridella) in her pupal stage in winter. the fungi were pathogenic against the hibernating pupae of cameraria ohridella at dose of 1.9 x 10(7) conidia/ml. they were aggressive, led to infection, death and mouldiness of naked pupae. even at low temperature of 5 degrees c and 12 degrees c. l. muscari ...200718399468
pathogenicity of entomopathogenic fungi on hibernating pupae of cameraria ohridella deschka & dimic 1986 (lepidoptera, gracillariidae). part 2: efficacy of entomopathogenic fungi against pupa in her pupal cell.the naked pupae of chestnut leafminer cameraria ohridella are susceptible for entomopathogenic fungi paecilomyces fumosoroseus strain p6; lecanicillium muscarium strain v24; metarhizium anisopliae strain m72 and beauveria bassiana strain b412. their activity at low temperature is advantageous especially for the use in the winter season. (richter et al., 2007a). therefore the possibility of infection should be examined in biotest for pupae in their pupal cells on leaf of chestnut at conditions li ...200718399469
perspectives for the biological control of cameraria ohridella.the horse chestnut leaf-miner, cameraria ohridella deschka et dimic (lepidoptera, gracillariidae) is a serious invasive pest of aesculus hippocastanum in europe. the larvae of this species feed on leaf parenchyma and can reduce the tree growth. we studied the impact of parasitoids on c. ohridella in the czech republic and also searched for entomopathogenic fungi associated with this pest. the results showed that the rate of parasitism varied between 5% and 15% in most cases. the most parasitized ...200718399483
isolation and characterization of an isaria fumosorosea isolate infecting the asian citrus psyllid in florida.a fungal pathogen that killed adult diaphorina citri kuwayama (asian citrus psyllid) (hemiptera: psyllidae) in florida citrus groves during the fall of 2005 was identified and characterized. investigation of this pathogen is important because d. citri vectors citrus greening disease (huanglongbing), which was reported in florida in 2005. the morphological and genetic data generated herein support identification of the fungus as isaria fumosorosea wize (ifr) (=paecilomyces fumosoroseus) (hypocrea ...200818433768
virulence of hypocreales fungi to pecan aphids (hemiptera: aphididae) in the laboratory.there is need for efficacious biocontrol agents for aphids in commercial orchards. as a preliminary step to this end we determined the virulence of several hypocreales fungi to pecan aphids. in the first experiment we tested the virulence of isaria fumosorosea (arsef 3581) blastospores to three pecan aphids monellia caryella, melanocallis caryaefoliae, and monelliopsis pecanis under laboratory conditions. rates of 1x10(7) or 1x10(8) spores per ml were applied in 2 ml via a spray tower to 90 mm p ...200818675272
effect of fungal infection on reproductive potential and survival time of tetranychus urticae (acari: tetranychidae).the effect of fungal infection on the reproductive potential of two-spotted spider mite, tetranychus urticae, was evaluated as part of the full biocontrol potential of three entomopathogenic fungi by modeling of fecundity probability. female mites (<or=2-day-old) on leaves were exposed to the sprays of beauveria bassiana, paecilomyces fumosoroseus and metarhizium anisopliae at the concentrations of 1.13 x 10(3), 1.55 x 10(3) and 0.95 x 10(3) deposited conidia mm(-2) and then individually reared ...200919153812
susceptibility of ceratitis capitata wiedemann (diptera: tephritidae) to entomopathogenic fungi.the mediterranean fruit fly ceratitis capitata (wiedemann) is one of the most serious pest of fruits in syria and present all year round. this fly is active on different host plants, such as mango, peach, fig, apple, pear, and citrus. the number of generations per year varies with local temperatures and host plant. the objective of this research was: to evaluate the pathogenicity of entomopathogenic fungi on adults of c. capitata under laboratory conditions. flies for experiments were obtained f ...200819226799
the role of antennae in removing entomopathogenic fungi from cuticle of the termite, coptotermes formosanus.our previous research has shown that the termite, coptotermes formosanus shiraki (isoptera: rhinotermitidae), protects itself from entomopathogenic fungi by mutual grooming behavior. the termite removes and discards foreign organisms, such as fungal conidia, from the body surface of its nestmates by mutual grooming behavior. the role of the antennae in detecting the condia was examind here. three entomopathogenic fungi were used, beauveria brongniartii 782 (saccardo) (hypocreales), paecilomyces ...200919611249
a beauvericin hot spot in the genus isaria.beauvericin is a naturally occurring cyclohexadepsipeptide originally described from beauveria bassiana but also reported from several fusarium species as well as members of the genus isaria. twenty-six isolates of isaria species and its cordyceps teleomorph, and ten taxonomically close strains including beauveria, nomuraea and paecilomyces species were sequenced and tested for beauvericin production. trees using its rdna and beta-tubulin sequence data were constructed and used to infer the phyl ...200919766720
entomopathogenic fungal activity against pupae and adult haematobia irritans (diptera: muscidae).the horn fly haematobia irritans is one of the most important ectoparasites associated with grazing bovines. this study investigated the pathogenic activity of metarhizium anisopliae (e9, ibcb425 and ibcb159), beauveria bassiana (jab06, jab07 and am09), isaria fumosorosea (=paecilomyces fumosoroseus) (ibcb133 and cb75) and isaria farinosa (=paecilomyces farinosus) (cg189 and cg195) fungi isolates on pupae and adult h. irritans. groups of 20 pupae and 30 adult flies were respectively bathed and s ...201019880254
efficiency of entomopathogenic fungi in the control of eggs and larvae of the horn fly haematobia irritans (diptera: muscidae).the present study assessed the pathogenic effect of isolates e9, ibcb425 and ibcb159 of the metarhizium anisopliae fungus, jab06, jab07 and am09 of beauveria bassiana, ibcb133 and cb75 of isaria fumosorosea (=paecilomyces fumosoroseus) and cg189 and cg195 of isaria farinosa (=paecilomyces farinosus) against eggs and larvae of the horn fly haematobia irritans. eggs were inoculated with suspensions containing 10(6), 10(7) and 10(8)conidiaml(-1) of the fungal isolates and observed after 48h to dete ...201019880255
hydrophobicity-related protein contents and surface areas of aerial conidia are useful traits for formulation design of fungal biocontrol agents.to clarify the potential use of hydrophobicity-related traits of aerial conidia in formulation design of fungal biocontrol agents, hydrophobicity rates (h (r)) and surface areas (s (a)) of aerial conidia were assessed with 48 strains of beauveria bassiana, isaria fumosorosea and metarhizium spp. inter- or intra-specific variation was large in h (r) (59.7-92.2%) and s (a) (7.9-25.3 microm(2) conidium(-1)) measurements, which were significantly correlated (r (2) = 0.55). six isolates of the three ...201020155444
production of thermotolerant entomopathogenic isaria fumosorosea sfp-198 conidia in corn-corn oil mixture.low thermotolerance of entomopathogenic fungi is a major impediment to long-term storage and effective application of these biopesticides under seasonal high temperatures. the effects of high temperatures on the viability of an entomopathogenic fungus, isaria fumosorosea sfp-198 (kctc 0499bp), produced on different substrates amended with various additives were explored. ground corn was found to be superior in producing the most thermotolerant conidia compared to yellow soybean, red kidney bean, ...201020157761
physiological effect of multi-walled carbon nanotubes (mwcnts) on conidia of the entomopathogenic fungus, paecilomyces fumosoroseus (deuteromycotina: hyphomycetes).the aim of the investigation was to assess the influence of commercial and carboxylated multi-walled carbon nanotubes (mwcnts) on conidia of the entomopathogenic fungus paecilomyces fumosoroseus. the commercial mwcnts had an external diameter of about 40-60 nm, a length of 300-600 nm, a specific density of 140 to 300 g/dm(3) and a carbon content of above 80%. carboxylated mwcnts were obtained by oxidizing commercial mwcnt by heating in hno(3), filtering, washing with water and drying. conidia af ...200920183518
persistence of isaria fumosorosea (hypocreales: cordycipitaceae) sfp-198 conidia in corn oil-based suspension.long-term persistence of entomopathogenic fungi as biopesticides is a major requirement for successful industrialization. corn oil carrier was superior in maintaining germination rates of isaria fumosorosea sfp-198 conidia during exposure to 50°c for 2 h, when compared with other oils, such as soybean oil, cottonseed oil, paraffin oil, and methyl oleate. the corn oil-based conidial suspension (91.6% germination) was also better in this regard than conidial powder (28.4% germination) after 50°c f ...201120571913
pathogenicity of some hypocrealean fungi to adult aedes aegypti (diptera: culicidae).the pathogenicity of 19 hypocrealean entomopathogenic fungi from seven different genera in adult aedes aegypti was tested. all fungi proved to be pathogenic, and isaria fumosorosea, lecanicillium muscarium, lecanicillium psalliotae, metarhizium anisopliae, metarhizium lepidiotae, metarhizium majus, metarhizium frigidum, paecilomyces carneus, and paecilomyces lilacinus caused total mortality within 15 days of exposure of mosquitoes to the fungal culture. all fungi developed on dead individuals. t ...201020680340
effects of fluctuating moisture and temperature regimes on the persistence of quiescent conidia of isaria fumosorosea.conidia of isaria fumosorosea were submitted to three regimes of temperature and moisture to simulate microclimatic conditions which prevail in temperate (43% rh and 28 degrees c to 98% rh and 15 degrees c), subtropical (75% rh and 35 degrees c to 98% rh and 25 degrees c), and arid areas (13% rh and 40 degrees c to 33% rh and 15 degrees c) with daily fluctuating cycles. germination, conidial viability, and virulence to spodoptera frugiperda larvae were less affected after 20 days exposure under ...201020685211
complementary effect of phloxine b on the insecticidal efficacy of isaria fumosorosea sfp-198 wettable powder against greenhouse whitefly, trialeurodes vaporariorum west.insecticidal activities of five photoactive dyes against greenhouse whitefly (gwf), trialeurodes vaporariorum west., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, isaria fumosorosea sfp-198 wettable powder (wp). azorubine, eosin b, erythrosine, brilliant green and phloxine b were used in this work, accompanying pyriproxyfen emulsifiable concentrate (ec) as a commercial standard for comparison.201020718026
influence of fungal odor on grooming behavior of the termite, coptotermes formosanus.the termite coptotermes formosanus shiraki (isoptera: rhinotermitidae) protects itself from entomopathogenic fungus by mutual grooming behavior. c. formosanus removes foreign organisms, such as fungal conidia, from the body surface of its nestmates by mutual grooming behavior and eating them. the conidia removal rate from the body surface differed according to the isolate of entomopathogenic fungi (beauveria brongniartii 782, paecilomyces fumosoroseus k3, and metarhizium anisopliae 455), and the ...201021073347
occupational exposure to microorganisms used as biocontrol agents in plant production.exposure to bioaerosols containing fungi and bacteria may cause various deleterious respiratory health effects. fungi and bacteria are commercially produced and applied to the environment as microbiological pest control agents (mpcas). attention has been drawn towards the exposure and health risks due to the use of commercially important mpcas. as part of a risk evaluation this paper intends to review whether the exposure to mpcas (beauveria bassiana, verticillium lecanii, trichoderma harzianum, ...201121196399
retracted article: stress response of entomopathogenic fungus isaria fumosorosea to copper. 201121221837
entomopathogenic fungus as a biological control for an important vector of livestock disease: the culicoides biting midge.the recent outbreak of bluetongue virus in northern europe has led to an urgent need to identify control measures for the culicoides (diptera: ceratopogonidae) biting midges that transmit it. following successful use of the entomopathogenic fungus metarhizium anisopliae against larval stages of biting midge culicoides nubeculosus meigen, we investigated the efficacy of this strain and other fungi (beauveria bassiana, isaria fumosorosea and lecanicillium longisporum) as biocontrol agents against ...201121264343
compatibility among entomopathogenic hyphocreales and two beneficial insects used to control trialeurodes vaporariorum (hemiptera: aleurodidae) in mediterranean greenhouses.the effect of the combined use of encarsia formosa or macrolophus caliginosus and one of three marketed mycoinsecticides, mycotal-« (leucanicillium muscarium-based), naturalis-lôäó (beauveria bassiana-based) and preferal-« (isaria fumosorosea-based), on the control of the whitefly, trialeurodes vaporariorum, was studied under laboratory and greenhouse conditions. the results of both types of tests, the bioassays and the greenhouse trials, for all combinations of e. formosa with each of the three ...201121679715
high resistance of isaria fumosorosea to carbendazim arises from the overexpression of an atp-binding cassette transporter (ift1) rather than tubulin mutation.probing possible mechanisms involved in the resistance of entomopathogenic fungus isaria fumosorosea to carbendazim fungicide.201222044666
evaluation of organically acceptable insecticides as stand-alone treatments and in rotation for managing yellowmargined leaf beetle, microtheca ochroloma (coleoptera: chrysomelidae), in organic crucifer production.background: yellowmargined leaf beetle, microtheca ochroloma, is the most damaging pest of organic crucifer crops in the southern united states. experiments were conducted over four growing seasons (2007-2010) in alabama to evaluate some botanical and microbial insecticides approved by the organic materials review institute (omri) against m. ochroloma in organically grown crucifer crops. insecticides evaluated included pyganic(®) (pyrethrum), aza-direct(®) (azadiractin), entrust(®) (spinosad) ...201222231993
characterization of paecilomycescinnamomeus from the camellia whitefly, aleurocanthus camelliae (hemiptera: aleyrodidae), infesting tea in japan.the whitefly, aleurocanthus camelliae kanmiya and kasai (hemiptera: aleyrodidae), is an invasive species in japan that was first discovered in 2004 on tea in kyoto. soon after its arrival epizootics of an entomopathogenic fungus were observed in populations of the whitefly in many tea-growing regions. here we identify this fungus as paecilomyces cinnamomeus (petch) samson and w. gams (hypocreales: clavicipitaceae) based on morphological characteristics and molecular analyses. this is the first r ...201222330093
virulence of isaria sp. and purpureocillium lilacinum to rhipicephalus microplus tick under laboratory conditions.rhipicephalus microplus (canestrini) is an ectoparasite accountable for great economic losses. the use of entomopathogenic fungi to control arthropods has shown promising responses. the present study evaluated the virulence of isaria farinosa (holmsk.) fr., isaria fumosorosea (wize) brown and smith, and purpureocillium lilacinum (=paecilomyces lilacinus) (thom.) samson to engorged females, eggs, and larvae of r. microplus. there were four treatment groups (10(5), 10(6), 10(7), and 10(8) conidia ...201222710525
evaluation of entomopathogenic fungi and a nematode against the soil-dwelling stages of the crane fly tipula paludosa.larvae of the crane fly tipula paludosa (diptera: nematocera) are economically important pests of grasslands, nurseries and organically grown crops. this study was conducted to evaluate several entomopathogenic fungal strains, the entomopathogenic nematode heterorhabditis bacteriophora and the organophosphate insecticide dursban wg (75% chlorpyrifos) against larval stages of t. paludosa under laboratory and greenhouse conditions.201222740286
composition and antimicrobial activity of fatty acids detected in the hygroscopic secretion collected from the secretory setae of larvae of the biting midge forcipomyia nigra (diptera: ceratopogonidae).the hygroscopic secretion produced by the secretory setae of terrestrial larvae of the biting midge forcipomyia nigra (winnertz) was analysed using gas chromatography coupled with mass spectrometry (gc-ms). the viscous secretion is stored at the top of each seta and absorbs water from moist air. gc-ms analyses (four independent tests) showed that the secretion contained 12 free fatty acids, the most abundant of which were oleic (18:1), palmitic (16:0), palmitoleic (16:1) and linoleic (18:2). oth ...201222781366
performance of two isolates of isaria fumosorosea from hot climate zones in solid and submerged cultures and thermotolerance of their propagules.isaria fumosorosea frequently causes mycosis of agricultural pests in the hot semiarid and dry tropical regions of mexico. because temperature tolerance restricts the use of fungal biopesticides, we investigated two isolates from these areas for possible development into mycoinsecticides for use in hot weather agricultural zones. we studied the effects of culture system (solid or submerged cultures) and temperature on the fungal growth, extracellular enzyme production, pathogenicity, and thermot ...201323065378
distribution, occurrence and natural invertebrate hosts of indigenous entomopathogenic fungi of central india.we report here that, during periodical surveys of insects inhabiting diverse habitats for the collection of entomopathogenic fungi; a large number of isolates were recovered belonging to seven species, from various regions of madhya pradesh and chhattisgarh forest areas and agricultural fields. the most common entomopathogenic fungi such as beauveria bassiana, nomuraea rileyi, paecilomyces farinosus and paecilomyces fumosoroseus were found to infect various insect hosts species naturally viz. hy ...201023100813
mortality and repellent effects of microbial pathogens on coptotermes formosanus (isoptera: rhinotermitidae).two entomopathogenic fungi, isaria fumosorosea and metarhizium anisopliae, and one bacterium, bacillus thuringiensis, were tested for their ability to cause mortality of formosan subterranean termites (fst), coptotermes formosanus (shiraki), after liquid exposure, and for their lack of propensity to repel fst.201223241169
control of key pecan insect pests using biorational pesticides.key pecan insect pests include the black pecan aphid, melanocallis caryaefoliae (davis), pecan weevil, curculio caryae (horn), and stink bugs (hemiptera: pentatomidae). alternative control tactics are needed for management of these pests in organic and conventional systems. our objective was to evaluate the potential utility of several alternative insecticides including three plant extract formulations, eucalyptus extract, citrus extract-8.92%, and citrus extract-19.4%, and two microbial insecti ...201323448039
an oil-based formulation of isaria fumosorosea blastospores for management of greenhouse whitefly trialeurodes vaporariorum (homoptera: aleyrodidae).to date, emphasis has been placed on studying the virulence of isaria fumosorosea conidia rather than the virulence of their blastospores. in the present study, a comparison was made of the efficacy of blastospores of i. fumosorosea (sfp-198) versus conidia against greenhouse whitefly (gwf) trialeurodes vaporarioum nymphs and thermotolerance at 50‚ÄČ°c. to improve thermotolerance and potency, the blastospores were suspended in corn oil with a wetting and an insect-filming agent.201323536463
[antioxidation and multidrug resistance of structure-similar atp-binding cassette transporter proteins in two fungal entomopathogens].structure-similar atp-binding cassette (abc) transporter proteins, bbt1 in beauveria bassiana (bb) and ift1 in isaria fumosorosea (if), were characterized.201323614237
antioxidant enzyme influences germination, stress tolerance, and virulence of isaria fumosorosea.antioxidizing enzymes (superoxide dismutase, catalase, and glutathione peroxidae) are important enzymatic systems used to degrade hydrogen peroxide into water and oxygen, thereby lowering intracellular hydrogen peroxide levels. entomopathogenic fungi display increased activities of antioxidizing enzymes during growth and germination, which is necessary to counteract the hyperoxidant state produced by oxidative metabolism. we studied the influence of different carbon sources on antioxidizing enzy ...201323756951
oxygen-rich culture conditions enhance the conidial infectivity and the quality of two strains of isaria fumosorosea for potentially improved biocontrol processes.in addition to high production levels of conidia, the success of entomopathogenic fungi as biological control agents depends both on their prevalence under the environmental conditions found in open fields (resistance to stress) and on the capacity of these conidia to infect pests. this study compares conidium production, infectivity and resistance to thermal and osmotic stress in two strains of isaria fumosorosea (arsef 3302 and cnrcb1) grown either under a normal atmosphere (21% o2) or using e ...201423847155
laboratory evaluation of isaria fumosorosea against spodoptera littoralis.isaria fumosorosea (syn. paecilomyces fumosoroseus) is potentially useful for the biological control of economically important agricultural and forest insect pests. we evaluated efficacy of two strains of this entomopathogenic fungus against last instar larvae and pupae of egyptian cotton leafworm, spodoptera littoralis. the first strain was apopka 97 which is an active ingredient of commercial biopesticide preferal wg (biobest, belgium). the second strain was isolated from cameraria ohridella a ...201223885438
evaluation of entomopathogenic fungi against chilli thrips, scirtothrips dorsalis.commercial strains of entomopathogenic fungi were evaluated for control of chilli thrips, scirtothrips dorsalis hood (thysanoptera: thripidae), an invasive pest of ornamental and vegetable plants in the caribbean and southeastern united states. in laboratory assays, lc50 values against adult s. dorsalis were 5.1 × 10(4) cfu/ml for beauveria bassiana gha, with higher values 3.1 × 10(5) for metarhizium brunneum f52 and 3.8 × 10(5) for isaria fumosorosea apopka 97. second instars were comparatively ...201323895429
the antifungal activity of fatty acids of all stages of sarcophaga carnaria l. (diptera: sarcophagidae).fatty acids as components of cuticular lipids of insects play a significant role in antifungal in protection against fungal infection. the chemical composition of cuticular and internal extracts obtained from all developmental stages of flesh flies sarcophaga carnaria was identified. the fatty acids were detected using gas chromatography coupled with mass spectrometry and the most abundant for all examined stages were: 18:1 > 16:0 > 16:1 > 18:0 > 18:2. polyunsaturated fatty acids (pufa) c20 were ...201423969191
combined effect of microbial and chemical control agents on subterranean termites.termite mortality was measured when fungi were combined with bacteria or a chemical termiticide to determine whether a synergistic effect occurred. the fungus beauveria bassiana was combined with the non-repellant chemical termiticide imidacloprid. of the three b. bassiana strains tested one, b. bassiana atcc 90519, was sufficiently pathogenic on its own that the advantage of a supplementary chemical treatment was marginal. the mortality caused by another fungal strain, b. bassiana atcc 26037, w ...201324037651
enhanced fungicide resistance in isaria fumosorosea following ionizing radiation-induced mutagenesis.the application of entomopathogenic fungi such as isaria fumosorosea to combat insect pests on plants is complicated by their sensitivity to commonly used fungicides. in this study, i. fumosorosea mutants with enhanced resistance to the fungicide benomyl were induced by irradiation using either ion beams or gamma rays, or a combination of the two. when grown on agar containing benomyl, mycelial growth was observed for five of the six mutant isolates at benomyl concentrations that were more than ...201324164561
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