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screening for <i>hepatozoon</i> parasites in gerbils and potential predators in south africa.samples of gerbils and their potential predators were screened for the presence of hepatozoon parasites (apicomplexa: adeleorina) using both microscopic examination and sequencing of partial 18s rrna sequences. positive samples were compared to published sequences in a phylogenetic framework. the results indicate that genets can be infected with hepatozoon felis. a cape fox was infected with hepatozoon canis, whereas the sequence from an infected rodent fell within a group of parasites primarily ...201728235289
hepatozoon spp. infects free-ranging jaguars (panthera onca) in brazil.this study investigated the presence of hepatozoon spp. in jaguars (panthera onca) and domestic animals in the cerrado, amazon and pantanal biomes of brazil. between february 2000 and january 2010, blood samples were collected from 30 jaguars, 129 domestic dogs (canis lupus familiaris) and 22 domestic cats (felis catus) for molecular tests. all of the jaguars from the pantanal (n=22) and cerrado (n=4) and 3 of 4 jaguars from the amazon were positive for hepatozoon spp. domestic dogs (62.8%) and ...201728207298
diagnosis of hepatozoon canis. 201728154224
molecular detection of hepatozoon spp. and cytauxzoon sp. in domestic and stray cats from madrid, spain.different species of apicomplexan protozoans of the genera hepatozoon and cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. the aim of this study was to assess the molecular prevalence of hepatozoon spp. and cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from madrid, spain.201728285590
splenitis in 33 dogs.splenitis is uncommonly reported in dogs. herein, the authors describe its prevalence, clinical findings and outcomes, histologic patterns, and causes. splenic samples of dogs diagnosed with splenitis between 2005 and 2013 were collected and stained with hematoxylin and eosin, gram, green-gram, giemsa, periodic acid-schiff, and ziehl-neelsen. samples were processed for polymerase chain reaction (pcr) to detect bacteria, fungi, and protozoa ( leishmania infantum, hepatozoon canis). thirty-three o ...201727337982
a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli.babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ...201728739301
a pcr survey of vector-borne pathogens in different dog populations from turkey.in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ...201728682765
a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia.canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ...201728673460
molecular detection of vector-borne pathogens in dogs and cats from qatar.vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries.201728633666
molecular detection of tick-borne pathogens in wild red foxes (vulpes vulpes) from central italy.spleen samples from 153 red foxes, shot during regular hunting season in the province of pisa (central italy), were examined to detect dna of anaplasma phagocytophilum, ehrlichia canis, coxiella burnetii, francisella tularensis, hepatozoon canis and babesia sp./theileria sp. dna of vector-borne pathogens was detected in 120 (78.43%; 95% ci: 71.06-84.66%) foxes. specifically, 75 (49%; 95% ci: 40.86-57.22%) animals scored pcr-positive per h. canis, 68 (44.44%; 95% ci: 36.42-52.69%) for e. canis, 3 ...201728502642
occurrence and diversity of arthropod-transmitted pathogens in red foxes (vulpes vulpes) in western austria, and possible vertical (transplacental) transmission of hepatozoon canis.red fox (vulpes vulpes) is the most abundant wild canid species in austria, and it is a well-known carrier of many pathogens of medical and veterinary concern. the main aim of the present study was to investigate the occurrence and diversity of protozoan, bacterial and filarial parasites transmitted by blood-feeding arthropods in a red fox population in western austria. blood (n = 351) and spleen (n = 506) samples from foxes were examined by pcr and sequencing and the following pathogens were id ...201728835291
detection and molecular characterization of babesia, theileria, and hepatozoon species in hard ticks collected from kagoshima, the southern region in japan.to reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (pcr) system to detect the most common agents of tick-borne parasitic diseases, namely babesia, theileria, and hepatozoon parasites. we collected host-seeking or animal-feeding ticks in kagoshima prefecture, the southernmost region of kyusyu island in southwestern japan. twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for ...201728501503
acute hepatozoonosis in dogs: a case report.two male dogs aged between 2 and 3 months belonging to german shepherd and non descript breed were brought to outpatient unit of department of veterinary medicine, veterinary college, bidar with history of anorexia, weakness and going down in body condition since 15 days. clinical examination revealed fever, enlargement of popliteal and submandibular lymph nodes, pale mucous membranes and cachectic body condition in both the cases. haemato-biochemical examination revealed anemia, leukocytosis, t ...201728848272
didelphis albiventris naturally infected with hepatozoon canis in southeastern brazil.hepatozoon species are vector-borne pathogens that infect domestic and wild animals. marsupials of the species didelphis albiventris are adapted to urban and peri-urban areas and act as reservoir hosts for several parasites. the present study evaluated the occurrence of infection by hepatozoon species in synantropic d. albiventris from botucatu, são paulo, brazil. blood samples and ectoparasites from 19 d. albiventris were collected from urban and peri-urban areas. hepatozoon spp. detection was ...201728728938
babesia, theileria, and hepatozoon species in ticks infesting animal hosts in romania.babesia spp., theileria spp., and hepatozoon spp. are tick-transmitted apicomplexan parasites that cause several important diseases in animals. to increase current knowledge about the diversity of tick-transmitted pathogens in romania, we investigated the occurrence of babesia spp., theileria spp., and hepatozoon spp. in a wide range of tick species infesting animal hosts. we collected 852 ticks from 10 different animal species from 20 counties in romania. the assessment was based on detection o ...201728676905
three different hepatozoon species in domestic cats from southern italy.three species of hepatozoon, namely, hepatozoon felis, hepatozoon canis and hepatozoon silvestris may affect domestic and/or wild felids. although hepatozoonosis has been documented in a wide range of mammal species, data on cats are limited. to investigate the occurrence of these pathogens in cats, blood samples were collected from animals living in three provinces of southern italy (bari, lecce, and matera), and molecularly analysed by pcr amplification and sequencing of segments of the 18s rr ...201728576619
transstadial transmission of hepatozoon canis by rhipicephalus sanguineus (acari: ixodidae) in field conditions.this study investigated possible transovarial and transstadial transmission of hepatozoon canis by rhipicephalus sanguineus (latreille) ticks collected from naturally infected dogs in a municipal dog shelter and the grounds of the shelter. four hundred sixty-five engorged nymphs were collected from 16 stray dogs that were found to be infected with h. canis by blood smear and pcr analyses and maintained in an incubator at 28 °c for moulting. four hundred eighteen nymphs moulted to adults 14-16 d ...201728399211
species of ticks and carried pathogens in owned dogs in spain: results of a one-year national survey.this study presents the results of a national survey in spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. in the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. the primary species of ticks were rhipicephalus sanguineus s.l. (53%), dermacentor reticulatus (9%), ixodes ricinus (9%), and i. hexagonus (4%). parasitism by two species of these ...201728188085
first detection of tick-borne pathogens of dogs from malta.the knowledge about the vector-borne infections in domestic dogs has been increasing worldwide. however no studies have been done on hard tick infestation and tick-borne pathogens (tbps) of dogs present in malta. therefore, a total of 99 dogs was selected and inspected between march and july 2013 in 18 urban and 17 rural areas on the islands of malta and gozo. all ticks were removed from the dogs and identified. blood samples were taken and tested for protozoa (babesia spp. and hepatozoon spp.) ...201728143697
canine tick-borne pathogens in cyprus and a unique canine case of multiple co-infections.canine tick-borne pathogens such as ehrlichia canis and hepatozoon canis are widespread in the mediterranean basin but have never been reported or investigated in cyprus. we describe herein the presence of canine tick-borne pathogens in three dogs with clinical signs compatible with vector-borne diseases from paphos area of cyprus. molecular and phylogenetic analysis revealed the presence of e. canis, anaplasma platys, h. canis, babesia vogeli and mycoplasma haemocanis in cyprus. one dog co-infe ...201728089651
canine tick-borne diseases in pet dogs from romania.tick-borne diseases are of substantial concern worldwide for animals as well as humans. dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. in addition, co-infections with two or more pathogens enhance this problem further. molecular methods are useful to disentan ...201728335825
molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease.the spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (vbp), which could have a fundamental role in the pathogenesis of splenic disease. this study aimed to evaluate certain vbp in samples from dogs with splenic lesions.201728285583
serological and molecular prevalence of canine vector-borne diseases (cvbds) in korea.previous surveys in dogs from korea indicated that dogs are exposed to a variety of vector- borne pathogens, but perception for a nation-wide canine vector-borne disease (cvbd) occurrence has been missing. we report here results of both serological and molecular prevalence studies for major cvbds of dogs from all over the south korean peninsula except for jeju island.201728298245
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