Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bradykinins and their cdna from piebald odorous frog, odorrana schmackeri, skin. | bradykinin, (des-arg(9))-bradykinin and bradykinyl-vapas, were identified in the skin secretion of the piebald odorous frog, odorrana schmackeri. using 3'- and 5'-race reactions, bradykinin precursor cdna was cloned and found to contain an open-reading frame of 311 amino acid residues. the preprobradykinin was found to consist of a putative signal peptide of approximately 20 amino acid residues, followed by seven tandem repeat coding domains of 43-44 amino acids. bradykinin and its c-terminal ex ... | 2003 | 12948838 |
| amphibian skin peptides and their corresponding cdnas from single lyophilized secretion samples: identification of novel brevinins from three species of chinese frogs. | brevinins are peptides of 24 amino acid residues, originally isolated from the skin of the oriental frog, rana brevipoda porsa, by nature of their microbicidal activity against a wide range of gram-positive and gram-negative bacteria and against strains of pathogenic fungi. cdna libraries were constructed from lyophilized skin secretion of three, unstudied species of chinese frog, odorrana schmackeri, odorrana versabilis and pelophylax plancyi fukienensis, using our recently developed technique. ... | 2006 | 16139929 |
| cloning from tissue surrogates: antimicrobial peptide (esculentin) cdnas from the defensive skin secretions of chinese ranid frogs. | the defensive skin secretions of amphibians are a rich source of bioactive peptides. here we describe a rapid technique for skin granular gland transcriptome cloning from a surrogate tissue-the secretion itself. cdna libraries were constructed from lyophilized skin secretion from each of the chinese frogs (rana schmackeri, rana versabilis, and rana plancyi fukienensis) using magnetic oligo(dt) bead-captured polyadenylated mrna as templates. specific esculentin cdnas were amplified by 3'-race usi ... | 2006 | 16427248 |
| auditory response characteristics of the piebald odorous frog and their implications. | the piebald odorous frog (odorrana schmackeri), the large odorous frog (odorrana livida) and the concave-eared torrent frog (amolops tormotus) are sympatric species living near the same torrent streams in the vicinity of mt. huangshan, china. a recent study demonstrated that a. tormotus can use sound signals involving ultrasonic components for communication in a noisy environment, and another sympatric species, o. livida, can also perceive ultrasonic sound. here we report data on the hearing ran ... | 2006 | 16583231 |
| a novel bradykinin-like peptide from skin secretions of rufous-spotted torrent frog, amolops loloensis. | a bradykinin-like peptide has been isolated from skin secretions of rufous-spotted torrent frog, amolops loloensis. this bradykinin-like peptide was named amolopkinin. its primary structure, rapvppgftpfr, was determined by edman degradation and mass spectrometry. it is structurally related to bradykinin-like peptides identified from skin secretions of other amphibians. amolopkinin is composed of 12 amino acid residues and is related to bradykinin composed of nine amino acid residues, identified ... | 2006 | 16793174 |
| bradykinin-related peptides, including a novel structural variant, (val1)-bradykinin, from the skin secretion of guenther's frog, hylarana guentheri and their molecular precursors. | multiple bradykinin-related peptides including a novel bradykinin structural variant, (val(1))-bradykinin, have been identified from the defensive skin secretion of guenther's frog, hylarana guentheri by a tandem mass spectrometry method. subsequently, four different preprobradykinin cdnas, which encoded multiple bradykinin copies and its structural variants, were consistently cloned from a skin derived cdna library. these preprobradykinin cdnas showed little structural similarity with mammalian ... | 2007 | 17321638 |
| a novel bradykinin-like peptide from skin secretions of the frog, rana nigrovittata. | a bradykinin-like peptide has been isolated from the skin secretions of the frog rana nigrovittata. this peptide was named ranakinin-n. its primary structure, raeavppgftpfr, was determined by edman degradation and mass spectrometry. it is structurally related to bradykinin-like peptides identified from skin secretions of other amphibians. ranakinin-n is composed of 13 amino acid residues and is related to the bradykinin identified from the skin secretions of odorrana schmackeri, which is compose ... | 2008 | 17994619 |
| skin bradykinin-related peptides (brps) and their biosynthetic precursors (kininogens): comparisons between various taxa of chinese and north american ranid frogs. | bradykinins and related peptides (brps) occur in the defensive skin secretions of many amphibians. here we report the structures of brps and their corresponding biosynthetic precursor cdnas from the chinese brown frog, rana chensinensis, and the north american leopard frog, lithobates pipiens. r. chensinensis skin contained four transcripts each encoding a different kininogen whose organizations and spectrum of encoded brps were similar to those reported for the pickerel frog, lithobates palustr ... | 2008 | 18164514 |
| novel brevinins from chinese piebald odorous frog (huia schmackeri) skin deduced from cloned biosynthetic precursors. | antimicrobial peptides represent the most characterized and diverse class of peptides within the defensive skin secretions of anuran amphibians. with an ever expanding database of primary structures, the current accepted rules for nomenclature have become increasingly difficult to apply to peptides whose primary structural attributes are either unique or that fall between those that define existing groups. an additional factor that adds to the confusion is the regular re-classification or revisi ... | 2008 | 18423796 |
| polymorphic microsatellite loci in chinese piebald odorous frog (odorrana schmackeri). | 2011 | 21873773 | |
| ptm-driven differential peptide display: survey of peptides containing inter/intra-molecular disulfide bridges in frog venoms. | amphibian defensive skin secretions are complex species-specific mixtures of biologically active molecules, including many uncharacterized peptides. many of these peptides are post-translationally modified and amongst the modifications discovered so far on amphibian defense peptides, disulfide bonds are quite frequently encountered. the presence of this ptm often complicates the ms-based sequencing. here we demonstrate a method to target peptides containing inter/intra-molecular s-s bonds applyi ... | 2012 | 22975675 |
| sex-biased dispersal of a frog (odorrana schmackeri) is affected by patch isolation and resource limitation in a fragmented landscape. | sex-biased dispersal is widespread in the animal kingdom and is affected by numerous factors including mating system, social factors and environmental conditions. unlike birds and mammals, there is no common trend in amphibians and explaining the direction and degree of sex-biased dispersal in species-specific cases is difficult. we conducted a study on dispersal of the chinese piebald odorous frog (odorrana schmackeri) in a fragmented landscape associated with dam construction. ten microsatelli ... | 2012 | 23094076 |
| vasorelaxin: a novel arterial smooth muscle-relaxing eicosapeptide from the skin secretion of the chinese piebald odorous frog (odorrana schmackeri). | the defensive skin secretions of amphibians are a rich resource for the discovery of novel, bioactive peptides. here we report the identification of a novel vascular smooth muscle-relaxing peptide, named vasorelaxin, from the skin secretion of the chinese piebald odorous frog, odorrana schmackeri. vasorelaxin consists of 20 amino acid residues, srvvkcsgfrpgspdsrefc, with a disulfide-bridge between cys-6 and cys-20. the structure of its biosynthetic precursor was deduced from cloned skin cdna and ... | 2013 | 23405205 |
| vicariance and its impact on the molecular ecology of a chinese ranid frog species-complex (odorrana schmackeri, ranidae). | paleogeological events and pleistocene climatic fluctuations have had profound influences on the genetic patterns and phylogeographic structure of species in southern china. in this study, we investigated the population genetic structure and phylogeography of the odorrana schmackeri species complex, mountain stream-dwelling odorous frogs, endemic to southern china. we obtained mitochondrial sequences (1,151bp) of the complete nd2 gene and two flanking trnas of 511 individuals from 25 sites for p ... | 2015 | 26394403 |
| screening method for the discovery of potential bioactive cysteine-containing peptides using 3d mass mapping. | animal venoms and toxins are a valuable source of bioactive peptides with pharmacologic relevance as potential drug leads. a large subset of biologically active peptides discovered up till now contain disulfide bridges that enhance stability and activity. to discover new members of this class of peptides, we developed a workflow screening specifically for those peptides that contain inter- and intra-molecular disulfide bonds by means of three-dimensional (3d) mass mapping. two intrinsic properti ... | 2015 | 26552389 |
| kunitzins: prototypes of a new class of protease inhibitor from the skin secretions of european and asian frogs. | amphibian skin secretions contain biologically-active compounds, such as anti-microbial peptides and trypsin inhibitors, which are used by biomedical researchers as a source of potential novel drug leads or pharmacological agents. here, we report the application of a recently developed technique within our laboratory to "shotgun" clone the cdnas encoding two novel but structurally-related peptides from the lyophilised skin secretions of one species of european frog, rana esculenta and one specie ... | 2016 | 27311856 |
| a structural and functional analogue of a bowman-birk-type protease inhibitor from odorrana schmackeri. | frog skin secretions contain complex peptidomes and peptidic protease inhibitors that are one of the biologically and structurally described groups of components. in the present study, by use of molecular 'shotgun' cloning and lc ms/ms fractionation sequencing, a novel bowman-birk-type heptadecapeptide (aalkgcwtksippkpcf-amide), named odorrana schmackeritrypsin inhibitor (osti), with a canonical cys6-cys16 disulfide bridge, was isolated and identified in piebald odorous frog (o. schmackeri) skin ... | 2017 | 28356487 |
| pharmacological effects of two novel bombesin-like peptides from the skin secretions of chinese piebald odorous frog (odorrana schmackeri) and european edible frog (pelophylax kl. esculentus) on smooth muscle. | bombesin-like peptides, which were identified from a diversity of amphibian skin secretions, have been demonstrated to possess several biological functions such as stimulation of smooth muscle contraction and regulation of food intake. here, we report two novel bombesin-like peptides, bombesin-os and bombesin-pe, which were isolated from odorrana schmackeri and pelophylax kl. esculentus, respectively. the mature peptides were identified and structurally confirmed by high performance scliquid chr ... | 2017 | 29065544 |
| morphological and molecular characterization of seuratascaris numidica (seurat, 1917) (ascaridida: ascarididae). | seuratascaris numidica (seurat, 1917) is a specialized nematode species parasitizing amphibians only. in the present study, the detailed morphology of this poorly known species was studied using light and scanning electron microscopy based on the newly material collected from hoplobatrachus chinensis (osbeck) (amphibia: anura) in china. we found that the relative length of intestinal caecum in our male specimens (representing 68.4-71.1% of oesophageal length) is slighter longer than the previous ... | 2018 | 29351063 |
| a hylarana latouchii skin secretion-derived novel bombesin-related pentadecapeptide (ranatensin-hla) evoke myotropic effects on the in vitro rat smooth muscles. | amphibians have developed successful defensive strategies for combating predators and invasive microorganisms encountered in their broad range of environments, which involve secretion of complex cocktails of noxious, toxic and diverse bioactive molecules from the skins. in recent years, amphibian skin secretions have been considered as an extraordinary warehouse for the discovery of therapeutic medicines. in this study, through bioactivity screening of the hylarana latouchii skin secretion-deriv ... | 2019 | 30959738 |
| enhanced antimicrobial activity of n-terminal derivatives of a novel brevinin-1 peptide from the skin secretion of odorrana schmackeri. | antimicrobial peptides (amps) are promising therapeutic alternatives compared to conventional antibiotics for the treatment of drug-resistant bacterial infections. however, the application of the overwhelming majority of amps is limited because of the high toxicity and high manufacturing costs. amphibian skin secretion has been proven to be a promising source for the discovery and development of novel amps. herein, we discovered a novel amp from the skin secretion of odorrana schmackeri, and des ... | 2020 | 32751489 |