Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| biosynthetic studies on acetosellin and structure elucidation of a new acetosellin derivative. | natural products from fungi, especially ascomycota, play a major role in therapy and drug discovery. fungal strains originating from marine habitats offer a new avenue for finding unusual molecular skeletons. here, the marine-derived fungus epicoccum nigrum (strain 749) was found to produce the azaphilonoid compounds acetosellin and 5',6'-dihydroxyacetosellin. the latter is a new natural product. the biosynthesis of these polyketide-type compounds is intriguing, since two polyketide chains are a ... | 2017 | 28081580 |
| investigation of the microbial communities colonizing prepainted steel used for roofing and walling. | microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. understanding the relevant organisms and the mechanisms by which colonization occurs would provide valuable information that can be subsequently used to design fouling prevention strategies. here, next-generation sequencing and microbial community finger printing (t-rflp) were used to study the community composition of microbes colonizing prepainted steel ro ... | 2016 | 27998037 |
| fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management. | fungal endophytes present in different asymptomatic grapevine plants (vitis vinifera l.) located in different vineyards within alentejo, a highly important viticulture region in portugal, were identified in this study. sampled grapevine plants included the three most representative cultivars in the region, syrah, cabernet sauvignon, and aragonez, growing under two different modes of management, conventional and biological. sixteen fungal taxa were identified through sequencing of the internal tr ... | 2016 | 27890088 |
| environmental stress and elicitors enhance taxol production by endophytic strains of paraconiothyrium variabile and epicoccum nigrum. | this study examined the effect of different elicitors (seven, different concentrations) and environmental factors (water activity (aw), ph) on taxol production by strains of two endophytic fungi, paraconiothyrium variabile and epicoccum nigrum, isolated from temperate yew trees. a defined liquid broth medium was modified with elicitors, solute aw depressors at different ph values. for p. variabile, the best elicitor was salicylic acid at 50mg/l which gave a taxol yield of 14.7±4.8μg/l. the study ... | 2016 | 27241294 |
| immunological consequences of intestinal fungal dysbiosis. | compared to bacteria, the role of fungi within the intestinal microbiota is poorly understood. in this study we investigated whether the presence of a "healthy" fungal community in the gut is important for modulating immune function. prolonged oral treatment of mice with antifungal drugs resulted in increased disease severity in acute and chronic models of colitis, and also exacerbated the development of allergic airway disease. microbiota profiling revealed restructuring of fungal and bacterial ... | 2016 | 27237365 |
| incidence and severity of leaf and fruit diseases of plums in latvia. | in the present study six plum orchards in latvia were examined during 2014. one orchard was commercial with integrated pest management (ipm) practices, one was with organic management, two orchards were scientific collections and in two orchards plums were grown as a minor crop, using ipm practices. the shot-hole disease (wilsonomyces carpophilus) and fruit rot were monitored in the field. samples of twigs and leaves were taken for further examination if some other disease symptoms were observed ... | 2015 | 27141740 |
| isolation, identification, and ecology of growth and taxol production by an endophytic strain of paraconiothyrium variabile from english yew trees (taxus baccata). | endophytic fungi were isolated/screened from temperate taxus baccata (yew) for production of the anti-cancer drug taxol. of 242 endophytic fungi isolated from surface-sterilised yew tree twig segments, only two produced taxol in a defined liquid medium; confirmed by hplc and lc-ms/ms analyses. they were identified by molecular means by sequencing of its1/its2 regions and shown to be paraconiothyrium variabile and epicoccum nigrum. they produced 1.75 and 1.32 μgl(-1) taxol respectively in a defin ... | 2015 | 26466877 |
| production and chemical characterization of pigments in filamentous fungi. | production of pigments by filamentous fungi is gaining interest owing to their use as food colourants, in cosmetics and textiles, and because of the important biological activities of these compounds. in this context, the objectives of this study were to select pigment-producing fungi, identify these fungi based on internal transcribed spacer sequences, evaluate the growth and pigment production of the selected strains on four different media, and characterize the major coloured metabolites in t ... | 2016 | 26341482 |
| fungi in a changing world: growth rates will be elevated, but spore production may decrease in future climates. | very little is known about the impact of climate change on fungi and especially on spore production. fungal spores can be allergenic, thus being important for human health. the aim of this study was to investigate how climate change influences the responsive ability of fungi by simulating differing environmental regimes. fungal species with high spore allergenic potential and atmospheric abundance were grown and experimentally examined under a variety of temperatures and different nutrient avail ... | 2015 | 25414142 |
| [diversity and community structure of endophytic fungi from taxus chinensis var. mairei]. | a total of 628 endophytic fungi were isolated from 480 tissue segments of needles and branches of taxus chinensis var. mairei. according to morphological characteristics and its sequences, they represented 43 taxa in 28 genera, of which 10 hyphomycetes, 20 coelomycetes, 12 ascomycetes and 1 unknown fungus. phomopsis mali was confirmed as the dominant species. in accordance with relative frequency, alternaria alternata, aureobasidium pullulans, colletotrichum boninense, c. gloeosporioides, epicoc ... | 2014 | 25345060 |
| plant-pathogenic fungi in seeds of different pea cultivars in poland. | legume crops are exposed to infection by fungal pathogens, which often results in contamination with mycotoxins. the aim of this study was to evaluate the level of field resistance/susceptibility of edible and fodder pea cultivars to the colonization of seeds by fungal pathogens in two subsequent seasons, as well as to identify the pathogens present in the seeds of the tested cultivars. alternaria spp. were the most common fungi isolated from pea seeds in both seasons, followed by fusarium spp., ... | 2014 | 25205690 |
| assessment of allergenicity to fungal allergens of rohtak city, haryana, india. | fungal spores are known as one of the important bioparticles causing allergic manifestation in human beings. hence, knowledge of season and prevalence of the airborne allergens to which the patients are exposed is a prerequisite for proper diagnosis and treatment of allergic disorders in hypersensitive individuals. keeping this in view, aerial survey was performed in the atmosphere of rohtak city for 2 consecutive years (march 2008-february 2010), using a volumetric petri plate sampler. a total ... | 2014 | 24988378 |
| essential oil composition, antioxidant and antifungal activities of salvia sclarea l. from munzur valley in tunceli, turkey. | the essential oil composition and in vitro antioxidant and antifungal activity of the salvia sclarea l. from munzur valley in tunceli, turkey were evaluated in this research. the in vitro antifungal activity of ethanol, hexane and aqueous extracts of s. sclarea against pathogen fungi epicoccum nigrum and colletotrichum coccodes were investigated. the essential oil of aerial parts of s. sclarea was obtained by hydrodistillation and was analysed by gc and gc—ms. total antioxidant status was determ ... | 2014 | 24929006 |
| protein kinase and hdac inhibitors from the endophytic fungus epicoccum nigrum. | a chemical investigation of the endophytic fungus epicoccum nigrum isolated from leaves of mentha suaveolens collected in morocco resulted in the isolation of five new polyketides, epicocconigrones a and b (1 and 2), 3-methoxyepicoccone b (3), 3-methoxyepicoccone (4), and 2,3,4-trihydroxy-6-(methoxymethyl)-5-methylbenzaldehyde (5), together with five known compounds (6-10). the structures of the new compounds were unambiguously determined by extensive analysis of the 1d and 2d nmr and mass spect ... | 2014 | 24328302 |
| epicoccum nigrum and cladosporium sp. for the treatment of oily effluent in an air-lift reactor. | the metalworking industry is responsible for one of the most complex and difficult to handle oily effluents. these effluents consist of cutting fluids, which provide refrigeration and purification of metallic pieces in the machining system. when these effluents are biologically treated, is important to do this with autochthonous microorganisms; the use of these microorganisms (bioaugmentation) tends to be more efficient because they are already adapted to the existing pollutants. for this purpos ... | 2013 | 24294260 |
| identification accuracy and diversity reproducibility associated with internal transcribed spacer-based fungal taxonomic library preparation. | this study investigated analytical parameters that are inherently relevant to identifying and quantifying fungal communities based on polymerase chain reaction amplicons. specifically, we evaluated the accuracy of the blastn-based identification for internal transcribed spacer (its) sequences generated from pure cultures, and quantified the reproducibility of relative abundances as well as α and β diversity measurements using duplicated environmental samples. the blastn-based method produced acc ... | 2014 | 24237760 |
| aerobic microorganisms associated with alfalfa leafcutter bees (megachile rotundata). | characterization of microorganisms associated with alfalfa leaf-cutter bee (megachile rotundata) nectar, pollen, provisions, larval guts, and frass (excreta) in alberta demonstrated a varied aerobic microflora. yeasts were isolated frequently from nectar, pollen, and provisions but rarely from guts or frass. the most prevalent yeast taxa were: candida bombicola, cryptococcus albidus, metschnikowia reukaufii, and rhodotorula glutinis. although few filamentous fungi were found in nectar, they were ... | 1993 | 24190009 |
| potential of epicoccum purpurascens strain 5615 aumc as a biocontrol agent of pythium irregulare root rot in three leguminous plants. | epicoccum purpurascens stain 5615 aumc was investigated for its biocontrol activity against root rot disease caused by pythium irregulare. e. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. the germination rate of the three species of legume seeds treated with a e. purpurascens homogenate increased significantly compared with the seeds infested with p. irregulare. no root rot symptoms were observed o ... | 2010 | 23956668 |
| spatial and temporal variation in fungal endophyte communities isolated from cultivated cotton (gossypium hirsutum). | studies of fungi in upland cotton (gossypium hirsutum) cultivated in the united states have largely focused on monitoring and controlling plant pathogens. given increasing interest in asymptomatic fungal endophytes as potential biological control agents, surveys are needed to better characterize their diversity, distribution patterns and possible applications in integrated pest management. we sampled multiple varieties of cotton in texas, usa and tested for temporal and spatial variation in fung ... | 2013 | 23776604 |
| [not available]. | the influence of moulded hay (alternaria alternata, epicoccum nigrum, mucor racemosus, ulocladium chartarum) and the efficiency of vitamin b1 substitution to cope these effects on rumen protozoa was investigated using the longterm rumen simulation technique (rusitec) for about 25 days. moulded hay affected medium-sized protozoa to a different extent (alternaria alternata: -16 %, epicoccum nigrum: -27 %, mucor racemosus: -9 %, ulocladium chartarum: +2 %). the vitamin b1 substitution had positive ... | 2000 | 23605525 |
| highly selective and asymmetric reductive biotransformation of α-ionone by epicoccum purpurascens. | the biotransformation of terpenoid c13 norisoprenoid (±)-α-ionone (1) using the plant pathogenic fungus epicoccum purpurascens as a biocatalyst was investigated for the production of useful novel organic compounds. there are no reported biotransformations using e. purpurascens. the biotransformation of compound 1 via reduction of the c-9 ketone position yielded α-ionol (2) as the major metabolic product. reduction of the racemic α-ionone [(-)-(6s)- and (+)-(6r)-] resulted in the exclusive format ... | 2013 | 23535310 |
| biosynthesis of silver nanoparticles by the endophytic fungus epicoccum nigrum and their activity against pathogenic fungi. | there is an enormous interest in developing safe, cost-effective and environmentally friendly technologies for nano-materials synthesis. in the present study, extracellular biosynthesis of silver nanoparticles was achieved by epicoccum nigrum, an endophytic fungus isolated from the cambium of phellodendron amurense. the reduction of the silver ions was monitored by uv-visible spectrophotometry, and the characterization of the ag nps was carried out by x-ray diffraction and transmission electron ... | 2013 | 23463299 |
| endophytic fungi from vitis labrusca l. ('niagara rosada') and its potential for the biological control of fusarium oxysporum. | we investigated the diversity of endophytic fungi found on grape (vitis labrusca cv. niagara rosada) leaves collected from salesópolis, sp, brazil. the fungi were isolated and characterized by amplified ribosomal dna restriction analysis, followed by sequencing of the its1-5.8s-its2 rdna. in addition, the ability of these endophytic fungi to inhibit the grapevine pathogen fusarium oxysporum f. sp herbemontis was determined in vitro. we also observed that the climatic factors, such as temperature ... | 2012 | 23315803 |
| epicoccum nigrum for biocontrol agents in vitro of plant fungal pathogens. | epicoccum nigrum strains were evaluated in vitro as potential biological agents for control of the growth of fusarium avenaceum, f. graminearum, f. oxysporum and botrytis cinerea. individual biotic effects of five strains of e. nigrum on the various fungi were determined using the biotic series method elaborated for fungi, on potato dextrose agar (pda - difco) medium. our results show that e. nigrum strains limited the growth of all isolates of fusarium spp., but not of those of botrytis cinerea ... | 2011 | 22702188 |
| epicoccum nigrum p16, a sugarcane endophyte, produces antifungal compounds and induces root growth. | sugarcane is one of the most important crops in brazil, mainly because of its use in biofuel production. recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. in spite of several studies carried out to define ... | 2012 | 22675473 |
| a brief review of other notable protein detection methods on acrylamide gels. | several methods have been described to stain proteins analyzed on acrylamide gels. these include ultrasensitive protein detection in one-dimensional and two-dimensional gel electrophoresis using a fluorescent product from the fungus epicoccum nigrum; a fluorescence-based coomassie blue protein staining; visualization of proteins in acrylamide gels using ultraviolet illumination; fluorescence visualization of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, non ... | 2012 | 22585527 |
| degradation of biogenic amines by vineyard ecosystem fungi. potential use in winemaking. | aims: to evaluate the ability of grapevine ecosystem fungi to degrade histamine, tyramine and putrescine in synthetic medium and in wines. methods and results: grapevine and vineyard soil fungi were isolated from four locations of spain and were subsequently identified by pcr. a total of 44 fungi were evaluated for in vitro amine degradation in a microfermentation system. amine degradation by fungi was assayed by reversed-phase (rp)-hplc. all fungi were able to degrade at least two different p ... | 2012 | 22268845 |
| biomedically important pathogenic fungi detection with volatile biomarkers. | volatile chemical profiles collected from the headspace of aspergillus fumigatus (a pathogenic fungus that causes invasive pulmonary aspergillosis, allergic bronchopulmonary aspergillosis and chronic fungal sinusitis) grown on media with the connective tissue protein elastin, found in lung tissue, contained a large abundance of the sesquiterpene farnesene (3,7,11-trimethyl-1,3,6,10-dodecatetraene) and, depending on extraction time and sorbent material, other farnesene isomers and sesquiterpenes ... | 2012 | 22233561 |
| differences in culturable microbial communities in bird nestboxes according to orientation and influences on offspring quality in great tits (parus major). | although bird-microbial interactions have become a topic of increasing research, the influence of nest-site characteristics, such as cavity orientation, on nest microbial communities in free-living passerines has not, to our knowledge, been investigated. this is despite the possibility of microbial differences explaining non-random patterns in nest-site selection and offspring quality, such as those exhibited by great tits (parus major). we swabbed great tit nestboxes that faced either south-sou ... | 2011 | 22183046 |
| environmental factors and interactions with mycobiota of grain and grapes: effects on growth, deoxynivalenol and ochratoxin production by fusarium culmorum and aspergillus carbonarius. | mycotoxigenic fungi colonizing food matrices are inevitably competing with a wide range of other resident fungi. the outcomes of these interactions are influenced by the prevailing environmental conditions and the competing species. we have evaluated the competitiveness of f. culmorum and a. carbonarius in the grain and grape food chain for their in vitro and in situ dominance in the presence of other fungi, and the effect that such interactions have on colony interactions, growth and deoxynival ... | 2010 | 22069589 |
| selection of strains of epicoccum purpurascens for tolerance to fungicides and improved biocontrol of sclerotinia sclerotiorum. | a wild-type isolate of epicoccum purpurascens was exposed to shortwave ultraviolet light. one of the resulting cultures (m-20-a) was grown on media amended with the fungicides iprodione or vinclozolin and fungicide-tolerant strains were obtained. several comparisons were made between new strains and the wild type. sporulation was improved compared with the wild type. strains varied in their tolerance to iprodione and vinclozolin but were not tolerant to the fungicide benomyl. strains r4000, 16-b ... | 1990 | 22049935 |
| black fungi and associated bacterial communities in the phyllosphere of grapevine. | in this study we investigate bacterial communities in association with an enriched black-fungal community in the plant phyllosphere to test whether these fungi create an environment for specific bacteria. under organic conditions of agriculture, grapevine plants (vitis vinifera) display an increased occurrence of the black fungi aureobasidium pullulans and epicoccum nigrum. their enrichment agrees with the tolerance of these fungi to copper and sulphate, both used as main fungicides in organic v ... | 2011 | 21944210 |
| Polyphasic analysis of intraspecific diversity in Epicoccum nigrum warrants reclassification into separate species. | Epicoccum nigrum Link (syn. E. purpurascens Ehrenb. ex Schlecht) is a saprophytic ascomycete distributed worldwide which colonizes a myriad of substrates. This fungus has been known as a biological control agent for plant pathogens and produces a variety of secondary metabolites with important biological activities as well as biotechnological application. E. nigrum produces darkly pigmented muriform conidia on short conidiophores on sporodochia and is a genotypically and phenotypically highly va ... | 2011 | 21853017 |
| infection of the sunagoke moss panels with fungal pathogens hampers sustainable greening in urban environments. | drought and heat tolerance of the sunagoke moss (racomitrium japonicum) and the low thermal conductivity of the dry moss tissue offer novel greening and insulation possibilities of roofs and walls to mitigate the heat island phenomenon in urban environments. however, damage may appear in the moss panels under humid conditions in japan. in this study we characterized fungi associated with the damaged areas of the sunagoke moss panels. fungi were identified by morphology and internal transcribed s ... | 2011 | 21624641 |
| structural characterization of extracellular polysaccharides produced by the marine fungus epicoccum nigrum jjy-40 and their antioxidant activities. | two extracellular polysaccharides, enp1 and enp2, were isolated from the fermentation liquid of the marine fungus epicoccum nigrum jjy-40 by anion-exchange chromatography and gel-filtration chromatography, and their structures were investigated using chemical and spectroscopic methods including methylation analysis and nmr spectroscopy. the results demonstrated that enp1 was composed of mannose, glucose, and galactose in the molar ratio of 5.0:2.1:1.0, and the main chain of the polysaccharide co ... | 2011 | 21279405 |
| interactions between 'candidatus phytoplasma mali' and the apple endophyte epicoccum nigrum in catharanthus roseus plants. | we investigated the ultrastructural and molecular interactions between 'candidatus phytoplasma mali' and the apple endophyte epicoccum nigrum in the experimental host catharanthus roseus to determine whether inoculation of endophyte could trigger defence reactions in the host. | 2011 | 21205105 |
| antimicrobial activity and biodiversity of endophytic fungi in dendrobium devonianum and dendrobium thyrsiflorum from vietman. | endophytic fungi are rich in orchids and have great impacts on their host plants. 53 endophytes (30 isolates from dendrobium devonianum and 23 endophytic fungi from d. thyrsiflorum) were isolated, respectively, from roots and stems of dendrobium species. all the fungi were identified by way of morphological and/or molecular biological methods. 30 endophytic fungi in d. devonianum were categorized into 11 taxa and 23 fungal endophytes in d. thyrsiflorum were grouped into 11 genera, respectively. ... | 2010 | 21181405 |
| screening, identification and evaluation of potential biocontrol fungal endophytes against rhizoctonia solani ag3 on potato plants. | rhizoctonia solani is an important soilborne pathogen of potato plants whose control typically depends on chemicals. here, we screened six fungal endophytes for the suppression of r. solani growth both in vitro and in a greenhouse. these isolates were identified using morphology and internal transcribed spacer regions of rdna as alternaria longipes, epicoccum nigrum, phomopsis sp., and trichoderma atroviride. both t. atroviride and e. nigrum showed significant in vitro inhibition of mycelial gro ... | 2010 | 20738401 |
| thiodiketopiperazines produced by the endophytic fungus epicoccum nigrum. | thirteen new thiodiketopiperazines, epicoccin i (1), ent-epicoccin g (2), and epicoccins j-t (3-13), together with six known diketopiperazines (14-19), have been isolated from the endophytic fungus epicoccum nigrum. the structures of 1, 2, and 10 were confirmed by x-ray crystallography, and the absolute configurations of 2, 4, 6, and 8 were assigned using moshers' method. compounds 2, 6, 12, and 17 showed potent activities in vitro against the release of beta-glucuronidase in rat polymorphonucle ... | 2010 | 20550196 |
| enhancing the adhesion of epicoccum nigrum conidia to peach surfaces and its relationship to the biocontrol of brown rot caused by monilinia laxa. | to find a formulation of epicoccum nigrum conidia that enhances its adhesion to peach surfaces and improves its biocontrol efficacy against brown rot caused by monilinia laxa. | 2010 | 20163488 |
| antimicrobial activity of endophytic fungi associated with orchidaceae in brazil. | the purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of orchidaceae collected in a brazilian tropical ecosystem. in total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude extracts. thirty-three percent of the isolates displayed antimicrobial activity against at least one target microorganism. the multivariate statistical analyses conducted indicate tha ... | 2009 | 20029530 |
| chemical characterization of phoma pomorum isolated from danish maize. | strains of the genus phoma are often isolated from various environmental samples including cereals and maize. in the present study we performed a chemical characterization of strains isolated from danish samples derived from whole plant material collected at harvest. all strains were isolated using a recently developed isolation medium and identified morphologically as p. pomorum. this species is placed in the phoma section peyronellaea and strains of other members in this section were also incl ... | 2010 | 19932925 |
| diketopiperazines from the cordyceps-colonizing fungus epicoccum nigrum. | new diketopiperazines, epicoccins e-h (1-4) and diphenylalazines a (5) and b (6), have been isolated from the solid-substrate fermentation culture of the cordyceps-colonizing fungus epicoccum nigrum. the structures of 1-6 were determined primarily by nmr experiments, and the structure of 1 was confirmed by x-ray crystallography. the absolute configurations of 3 and 5 were assigned using the modified mosher (3) and marfey's (5) methods, respectively. compounds 3-5 showed inhibitory effects on hiv ... | 2009 | 19919067 |
| intraspecific variation and interspecific differences in the bacterial and fungal assemblages of blue tit (cyanistes caeruleus) and great tit (parus major) nests. | although interest in the relationship between birds and microorganisms is increasing, few studies have compared nest microbial assemblages in wild passerines to determine variation within and between species. culturing microorganisms from blue tit (cyanistes caeruleus) and great tit (parus major) nests from the same study site demonstrated diverse microbial communities with 32 bacterial and 13 fungal species being isolated. dominant bacteria were pseudomonas fluorescens, pseudomonas putida, and ... | 2010 | 19830477 |
| purification and immunological characterization of a 12-kda allergen from epicoccum purpurascens. | exposure to epicoccum purpurascens is implicated in respiratory allergies and asthma. several allergens of clinical importance were identified in epicoccum extract (ee), but only one allergen has been isolated and characterized. in the present study, a 12-kda allergen was isolated from an epicoccum spore-mycelial extract by concanavalin-a sepharose, reverse-phase hydrophobic and gel filtration chromatography. the purified protein was recognized as a single 12-kda allergen on immunoblot with a se ... | 2009 | 19239492 |
| population dynamics of epicoccum nigrum, a biocontrol agent against brown rot in stone fruit. | to study the population dynamics of epicoccum nigrum on peaches and nectarines and to enhance its colonization on fruit surfaces to improve its biocontrol efficacy against brown rot. | 2009 | 19200324 |
| potato carrot agar with manganese as an isolation medium for alternaria, epicoccum and phoma. | a semi-selective medium for isolation of alternaria spp., epicoccum sp. and phoma spp. from soil and plant samples was developed. the basal medium was a modified potato carrot agar (pca), containing 10 g/l of potato and carrot. it is known that the target genera sporulate well on standard pca when grown at 25 degrees c with an alternating light/dark cycle consisting of 8 h of cool-white daylight followed by 16 h darkness. addition of 1.5% mncl(2) 4 h(2)o (w/v) inhibited most other fungi than alt ... | 2009 | 19185378 |
| effect of proteolytic activity of epicoccum purpurascens major allergen, epi p 1 in allergic inflammation. | enzymes play an important role in inducing airway inflammation, but knowledge is limited to few proteins. this study was carried out to assess the role of epi p 1, a serine protease of epicoccum purpurascens, in inducing allergy and inflammation in a murine model. balb/c mice were sensitized with epi p 1 active protease (eap) or epicoccum extract. subsequently, epi p 1 sensitized mice were boosted on day 14 with eap or inactivated protease (eiap). three intranasal challenges were given and mice ... | 2008 | 18782325 |
| evaluation of epicoccum nigrum for growth, morphology and production of natural colorants in liquid media and on a solid rice medium. | four nonpathogenic and nontoxigenic epicoccum nigrum strains were evaluated for their growth, morphology and pigment producing ability in three complex and one defined liquid media. epicoccum nigrum ibt 41028 produced pigments in all the four media tested with a maximum pigment of 3.68 au at 410 nm in m1 medium (unoptimized) containing 5 g/l yeast autolysate. the color hue of the crude pigment extracts ranged from 74 to 102 exhibiting dark orange to green-yellow color. pelleted morphology was sh ... | 2008 | 18629439 |
| use of (1-3)-beta-d-glucan concentrations in dust as a surrogate method for estimating specific fungal exposures. | indoor exposure to fungi has been associated with respiratory symptoms,often attributed to their cell wall component, (1-3)-beta-d-glucan. performing(1-3)-beta-d-glucan analysis is less time consuming and labor intensive than cultivation or microscopic counting of fungal spores. this has prompted many to use(1-3)-beta-d-glucan as a surrogate for fungal exposure. the aim of this study was to examine which indoor fungal species are major contributors to the (1-3)-beta-d-glucan concentration in fie ... | 2008 | 18429996 |
| remarkable microfungi from oaxaca (mexico) of acacia species. | in the state of oaxaca (mexico, 10 km north-west of puerto escondido 15 degrees 55' n, 97 degrees 09' w) we were able to collect some microfungi living as parasites or saprophytes on acacia species, some of them are causing attention for oaxaca. many belong to the deuteromycotina (hyphomycetes, coelomycetes) and ascomycotina. on a. hindsii: calonectria pseudopeziza (desm.) sacc., hypoxylon truncatum (schwein. fr.) j.h. miller, epicoccum nigrum link., zygosporium gibbum (sacc., m. roussau & e. bo ... | 2007 | 18396821 |
| identification of epicoccum purpurascens allergens by two-dimensional immunoblotting and mass spectrometry. | sensitization to allergens of epicoccum purpurascens is reported from many places world over. previously, the ige binding proteins of epicoccum were studied by one-dimensional immunoblotting (1-de). however, the allergens recognized by 1-de may be a mixture of proteins appearing at the same molecular weight. in the present study, ige-binding components of e. purpurascens were detected using two-dimensional immunoblotting and mass spectrometry. approximately 250 protein spots were identified by c ... | 2008 | 18207028 |
| epicoccins a-d, epipolythiodioxopiperazines from a cordyceps-colonizing isolate of epicoccum nigrum. | epicoccins a-d (1-4), four unique epipolythiodioxopiperazines possessing unusual sulfur bridges, have been isolated from cultures of a cordyceps-colonizing isolate of epicoccum nigrum. the structures of these compounds were determined mainly by analysis of their nmr spectroscopic data. the relative and absolute configuration of epicoccin a (1) was established by single-crystal x-ray diffraction analysis. epicoccin a (1) showed modest antimicrobial activity. | 2007 | 17711348 |
| role of glycoproteins isolated from epicoccum purpurascens in host-pathogen interaction. | attachment to host matrix is an important provisory step for the institution of any fungal infection. the present study investigates the role of glycoproteins of epicoccum purpurascens in host-fungal adherence. | 2007 | 17643064 |
| [putative mycotoxin-producing fungi isolated from alpataco (prosopis flexuosa) fruits]. | fungi contaminant of alpataco (prosopis flexuosa) fruits from la pampa province (argentina) were identified. alternaria alternata and sphaeropsis sapinea were the dominant species. phoma sp., nigrospora sp., preussia minima, cladosporium sp., pithomyces chartarum, epicoccum nigrum, aspergillus niger and aspergillus speluneus were also isolated but with less frequency. twelve strains of alternaria alternata, the toxigenic species with higher incidence, were screened for alternariol (aoh), alterna ... | 2007 | 17592896 |
| occurence of fungal diseases on lemon balm (mellisa officinalis l.) and peppermint (mentha x piperita l.) in the region of malopolska. | this paper presents the results of the researches carried out on the subject of the diseases on herbs such as lemon balm and peppermint focusing on the health status of the plants grown in the region of malopolska. the field and laboratory research proved that perpetrators of the diseases on the examined plants were fungi species with the numerical majority. on lemon balm septoria leaf spot (septoria melissae) was most often observed. moreover, fungal genera of different taxonomic groups were de ... | 2006 | 17390866 |
| effects of stabilizers on shelf-life of epicoccum nigrum formulations and their relationship with biocontrol of postharvest brown rot by monilinia of peaches. | to find a formulation of epicoccum nigrum conidia that maintains a high viability over time and which proves efficient to biocontrol peach rot caused by monilinia spp. | 2007 | 17241364 |
| halogen immunoassay, a new method for the detection of sensitization to fungal allergens; comparisons with conventional techniques. | accurate diagnosis of allergy to specific fungal species is confounded by the variability in allergens occurring with different diagnostic systems. we compared the halogen immunoassay (hia), which uses allergens expressed by freshly germinated spores that are bound to protein binding membranes (pbm), with the commercial pharmacia unicap assay (cap) and with skin prick tests (spt). | 2006 | 17075249 |
| influence of harvest date and geographical location on kernel symptoms, fungal infestation and embryo viability of malting barley. | this study focused on the influence of harvest timing and geographical location on spring malting barley (potential malting variety) in terms of fungal infestation and seedling viability in symptomatic and asymptomatic kernels. the study was conducted over two consecutive crop years (2003-2004) at four locations in slovakia. the following categories of the barley kernels were used for the assessment: kernels with black point symptoms (bpk), kernels with black cover on their surface (bck), pink-c ... | 2007 | 16962194 |
| structure and assembly of epiglucan, the extracellular (1-->3;1-->6)-beta-glucan produced by the fungus epicoccum nigrum strain f19. | in a previous article [carbohydr. res.2001, 331, 163-171] two different structures for the possible modular repeating unit of the extracellular beta-glucan, epiglucan produced by the fungus epicoccum nigrum strain f19 were proposed. clarifying which was the more likely one was considered essential before attempts were made to understand how epiglucan was assembled by this fungus. data from smith degradation analyses of epiglucan were consistent with the repeating unit of structure i, where singl ... | 2006 | 16359651 |
| growth and pigmentation of epicoccum nigrum in submerged culture. | growth and pigmentation of epicoccum nigrum was studied in submerged culture in various media. red pigmentation of the mycelium and of fermentation broth was obtained only in a medium containing glucose and yeast autolysate. this pigmentation occurred at the time of maximal production of carotenoids. observations on the submerged culture of this organism in flasks and in fermentors are described, and the details of a standardized procedure for the production of carotenoid pigments are given. | 1969 | 16349848 |
| production of mannitol by fungi from cotton dust. | cotton dust associated with high pulmonary function decrements contains relatively high levels of mannitol. in this study, cotton leaf and bract tissue and dust isolated from cotton leaf tissue were examined by optical microscopy, scanning electron microscopy, and capillary gas chromatography. alternaria alternata, cladosporium herbarum, epicoccum purpurascens, and fusarium pallidoroseum were isolated from cotton leaf dust. the fungal samples, cotton dust, and cotton leaf contained mannitol. thi ... | 1988 | 16347688 |
| epicocconone, a new cell-permeable long stokes' shift fluorescent stain for live cell imaging and multiplexing. | epicocconone is a heterocyclic natural product from the fungus epicoccum nigrum that fluoresces weakly in the green (520 nm). however, cells exposed to epicocconone rapidly absorb the dye and become bright orange fluorescent because the natural product reacts reversibly with proteins. the orange fluorescence is enhanced in lipophilic environments, allowing the visualization of membranous organelles and lipid rafts but does not stain oligonucleotides. as the unconjugated dye has no orange fluores ... | 2006 | 16328703 |
| extracellular abts-oxidizing activity of autochthonous fungal strains from argentina in solid medium. | the screening for extracellular oxidases and peroxidases from autochthonous filamentous fungi isolated from different substrates is an important step towards the detection of extracellular fungal oxidative systems. thirty-one autochthonous fungal strains from argentina, belonging to different ecophysiological and taxonomic groups, were plate-screened for their ability to produce extracellular oxidoreductases. modified kirk solid medium containing the chromogen 2,2-azino-bis(3-ethylbenzothiazolin ... | 2000 | 15813698 |
| immunobiochemical analysis of cross-reactive glutathione-s-transferase allergen from different fungal sources. | clinical observations suggest the presence of cross-reactive allergens. there is a need to identify these cross-reactive allergens to improve the treatment used for allergic disorders. the present study was aimed to identify and characterize a cross-reactive allergenic protein from fungi. allergen extracts of various fungi viz. alternaria alternata, aspergillus fumigatus, cladosporium herbarum, curvularia lunata, and epicoccum purpurascens showed gst enzymatic activity ranging from 0.765 to 1.00 ... | 2005 | 15773571 |
| allergens of the entomopathogenic fungus beauveria bassiana. | background: beauveria bassiana is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. although reported to be non-toxic to vertebrates, the potential allergenicity of beauveria species has not been widely studied. methods: ige-reactivity studies were performed using sera from patients displaying mould hypersensitivity by immunoblot and immunoblot inhibition. skin reactivity to b. bassiana extracts was measured using intradermal s ... | 2005 | 15644142 |
| antifungal activity of camptothecin, trifolin, and hyperoside isolated from camptotheca acuminata. | leaf spots and root rots are major fungal diseases in camptotheca acuminata that limit cultivation of the plant for camptothecin (cpt), a promising anticancer and antiviral alkaloid. bioassays showed that pure cpt and flavonoids (trifolin and hyperoside) isolated from camptotheca effectively control fungal pathogens in vitro, including alternaria alternata, epicoccum nigrum, pestalotia guepinii, drechslera sp., and fusarium avenaceum, although antifungal activity of these compounds in the plant ... | 2005 | 15631505 |
| metabiotic associations of molds and salmonella poona on intact and wounded cantaloupe rind. | salmonella poona, a serotype rarely implicated in human infections, has recently caused several cantaloupe-associated outbreaks of salmonellosis. metabiotic associations of molds and foodborne pathogens on produce have been reported. we tested proteolytic activity and measured changes in the ph of cantaloupe rind caused by growth of alternaria alternata, cladosporium cladosporioides, epicoccum nigrum, geotrichum candidum, and penicillium expansum. survival and growth characteristics of salmonell ... | 2005 | 15582743 |
| epi p 1, an allergenic glycoprotein of epicoccum purpurascens is a serine protease. | epicoccum purpurascens (ep) is a ubiquitous saprophytic mould, the inhalant spores and mycelia of which are responsible for respiratory allergic disorders in 5-7% of population worldwide. the diagnosis/therapy of these disorders caused by fungi involves the use of standardized and purified fungal extracts. a 33.5 kda glycoprotein, epi p 1 released histamine from whole blood cells of ep allergic patients at a concentration of 50-ng protein. the high specific ige values detected in ep hypersensiti ... | 2004 | 15364105 |
| solid substrate production of epicoccum nigrum conidia for biological control of brown rot on stone fruits. | production of conidia of epicoccum nigrum, a biocontrol agent of the fungal pathogen monilinia laxa, was tested in liquid- and solid-state fermentation. liquid fermentation was conducted in 250 ml erlenmeyer flasks containing 50 ml of a mineral medium (containing per litre: 20 g lactose, 10 g no3k, 1 g k2hpo4, 0.5 g mgso4.7h2o, and 1 ml of a minor-element solution), inoculated with 2 x 10(5) e. nigrum conidia ml(-1), and incubated at 20-25 degrees c and 150 rpm for 7 days. solid-state fermentati ... | 2004 | 15193802 |
| [from when on can fungi be identified in nasal mucus of humans?]. | fungal spores are frequent in air and their occurrence in the nasal mucus appears to be a common finding within the adult population, as we were able to show in recent studies. 91,3 % of crs patients but also healthy controls grew positive fungal cultures out of their nasal mucus. the potential role of fungal elements in nasal mucus for the pathogenesis of crs, with or without polyposis, is currently investigated intensely and discussed very controversially. however, it was still unknown, as of ... | 2004 | 14999588 |
| purification and characterization of a major cross-reactive allergen from epicoccum purpurascens. | epicoccum purpurascens (formerly nigrum) (ep), is a ubiquitous saprophytic mould found both indoors and outdoors. several studies have reported sensitization to ep in 5-7% of different populations worldwide. the diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. the crude allergen extract from ep was standardized earlier, however none of its allergens have been purified. | 2004 | 14976389 |
| stimulation of astaxanthin formation in the yeast xanthophyllomyces dendrorhous by the fungus epicoccum nigrum. | a fungal contaminant on an agar plate containing colonies of xanthophyllomyces dendrorhous markedly increased carotenoid production by yeast colonies near to the fungal growth. spent-culture filtrate from growth of the fungus in yeast-malt medium also stimulated carotenoid production by x. dendrorhous. four x. dendrorhous strains including the wild-type ucd 67-385 (atcc 24230), af-1 (albino mutant, atcc 96816), yan-1 (beta-carotene mutant, atcc 96815) and cax (astaxanthin overproducer mutant) ex ... | 2004 | 14734032 |
| a fluorescent natural product for ultra sensitive detection of proteins in one-dimensional and two-dimensional gel electrophoresis. | lightning fast is a sensitive fluorescence-based stain for detecting proteins in one-dimensional and two-dimensional polyacrylamide electrophoresis gels. it contains the fluorophore epicocconone from the fungus epicoccum nigrum that interacts noncovalently with sodium dodecyl sulfate and protein. stained proteins can be excited optimally by near-ultraviolet light of about 395 nm or with visible light of about 520 nm. the stain can be excited using a range of sources used in image analysis system ... | 2003 | 14673778 |
| identification and population dynamics of yeasts in sourdough fermentation processes by pcr-denaturing gradient gel electrophoresis. | four sourdoughs (a to d) were produced under practical conditions, using a starter obtained from a mixture of three commercially available sourdough starters and baker's yeast. the doughs were continuously propagated until the composition of the microbiota remained stable. a fungi-specific pcr-denaturing gradient gel electrophoresis (dgge) system was established to monitor the development of the yeast biota. the analysis of the starter mixture revealed the presence of candida humilis, debaryomyc ... | 2003 | 14660398 |
| epicoccamide, a novel secondary metabolite from a jellyfish-derived culture of epicoccum purpurascens. | from the inner tissue of the jellyfish aurelia aurita a marine strain of the fungus epicoccum purpurascens was obtained. after mass cultivation the fungus was investigated for its secondary metabolite content and found to contain the new, and most unusual tetramic acid derivative, epicoccamide (1). epicoccamide is quite unusual since it is composed of three biosynthetically distinct subunits; glycosidic, fatty acid and tetramic acid (amino acid). the structure of the new compound was elucidated ... | 2003 | 12926253 |
| epicocconone, a novel fluorescent compound from the fungus epicoccumnigrum. | epicocconone represents a new class of natural fluorescent probes based on a polyketide skeleton isolated from the fungus epicoccum nigrum. epicocconone is a small, cell permeable natural product with a high molar absorbtivity and a long stokes' shift that will be useful in biotechnological applications. | 2003 | 12889954 |
| water availability affects the growth, accumulation of compatible solutes and the viability of the biocontrol agent epicoccum nigrum. | growth of the biocontrol fungus epicoccum nigrum was more sensitive to ionic solute water stress (nacl) than non-ionic (glycerol) on potato dextrose-based media at -0.5, -3.0 and -5.5 mpa water potentials. subsequent physiological manipulation of growth of e. nigrum in glycerol-modified media to -3.0 mpa water potential resulted in a significant increase in the accumulation of compatible solutes in both mycelial liquid cultures and spores, but no enhanced accumulation of the desiccation protecta ... | 2003 | 12733630 |
| purification and characterization of a cross-reactive 45-kd major allergen of fusarium solani. | fusarium solani (fs) is an important source of fungal allergen. a 45-kd major allergen of fs showed reactivity with patients' sera sensitive to many fungi. | 2003 | 12660423 |
| allergen detection from 11 fungal species before and after germination. | allergens dispersed by airborne fungal spores play an important but poorly understood role in the underlying cause and exacerbation of asthma. previous studies suggest that spores of alternaria and aspergillus release greater quantities of allergen after germination than before germination. it is unknown whether this is true of other allergenic fungi. | 2003 | 12589346 |
| drying of epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease. | the effects of freeze-drying, spray-drying and fluidized bed-drying on survival of epicoccum nigrum conidia were compared. | 2003 | 12588560 |
| synthesis of two isomeric pentasaccharides, the possible repeating unit of the beta-glucan from the micro fungus epicoccum nigrum ehrenb. ex schlecht. | two isomeric pentasaccharides, beta-d-glcp-(1-->3)-[beta-d-glcp-(1-->6)]-beta-d-glcp-(1-->3)-[beta-d-glcp-(1-->6)]-beta-d-glcp (i) and beta-d-glcp-(1-->6)-beta-d-glcp-(1-->3)-[beta-d-glcp-(1-->3)-beta-d-glcp-(1-->6)]-beta-d-glcp (ii), the possible repeating unit of the beta-glucan from the micro fungus epicoccum nigrum ehrenb. ex schlecht, were synthesized as their 4-methoxyphenyl glycosides in a regio- and stereoselective manner. the pentasaccharide i was obtained from 3-o-selective glycosylati ... | 2002 | 12493222 |
| development of a selective myclobutanil agar (mba) medium for the isolation of fusarium species from asparagus fields. | a new selective myclobutanil agar medium for the detection of fusarium, species is proposed. ten media formulations based on various selective agents (pentachloronitrobenzene (pcnb), rose bengal, malachite green, sodium hypochlorite, captan, benomyl, chlorotalonil, myclobutanil, thiram, and cupric sulfate) were compared. first, mycelium growth and colony appearance of alternaria alternata, aspergillus flavus, cladosporium cladosporioides, epicoccum nigrum, fusarium sp., fuisarium solani, fusariu ... | 2002 | 12455616 |
| antigenic and allergenic cross-reactivity of epicoccum nigrum with other fungi. | previous studies have identified epicoccum nigrum (en) as an important aeroallergen. shared allergenicity among some fungi responsible for type i allergic disorders has been reported. | 2002 | 12269649 |
| covalent immobilization of proteins onto photoactivated polystyrene microtiter plates for enzyme-linked immunosorbent assay procedures. | enhancement of the speed and sensitivity of an elisa technique was achieved by doing it on a polystyrene microtiter plate preactivated by a simple photochemical reaction. immobilization of epicoccum nigrum antigen (allergenic antigen) or goat anti-rabbit igg onto the photoactivated plates was found to occur in only 45 min with higher binding than that obtained through adsorption during the same period onto the untreated surface. nearly 1.5-2-folds higher readings were obtained when the elisa was ... | 2002 | 12215385 |
| culture filtrate antigens and allergens of epicoccum nigrum cultivated in modified semi-synthetic medium. | epicoccum nigrum (en) is an important fungal allergen for nasobronchial allergy. fungal extracts should contain all the relevant allergen components from spores, mycelium and culture medium for the purpose of allergy diagnosis and therapy. en extract from spore-mycelial mass has been standardized, but the culture filtrate (cf) allergens of en have not been studied as en grows poorly in synthetic medium. the objective of the present study was to obtain a standard cf extract of en by cultivating t ... | 2002 | 12137194 |
| comparison of genotypic and phenotypic techniques for assessing the variability of the fungus epicoccum nigrum. | the diversity within a collection of worldwide isolates of epicoccum nigrum has been studied using several phenotypic approaches. in addition, the abilities of phenotypic and genotypic techniques for the differentiation of a set of isolates are compared. | 2002 | 12067372 |
| structure of epiglucan, a highly side-chain/branched (1 --> 3;1 --> 6)-beta-glucan from the micro fungus epicoccum nigrum ehrenb. ex schlecht. | the extracellular fungal polysaccharide, epiglucan, synthesised by epicoccum nigrum is a side-chain/branched (1 --> 3;1 --> 6)-d-beta-glucan. methylation analysis, 13c dept nmr and specific enzymic digestion data show slight variation in branching frequency among the epiglucans from the three strains examined. the (1 --> 3)-beta-linked backbone has (1 --> 6)-beta-linked branches at frequencies greater than the homologous glucans, scleroglucan and schizophyllan, from sclerotium spp. and schizophy ... | 2001 | 11322730 |
| the effect of non-pathogenic phylloplane fungi on life-history traits of urticae (acari: tetranychidae). | the direct effect of three common non-pathogenic phylloplane fungi on the life-history traits of tetranychus urticae koch was investigated on intact leaves of the garden bean phaseolus vulgaris l. (var. tendergreen improved), under conditions of low and high water-deficit stress. the survival rate of t. urticae was always reduced by an increase in water-deficit stress, whereas the effect of the fungi depended in part on the watering regime. on two of the three fungi tested, alternaria alternata ... | 2000 | 11110237 |
| sensitivity to fungal allergens is a risk factor for life-threatening asthma. | previous studies have suggested that sensitivity to alternaria and cladosporium may be risk factors for life-threatening asthma. we have investigated this by studying the relationship between skin tests for fungal spores and admission to an intensive care unit (icu) for asthma. | 2000 | 10843433 |
| allergens of epicoccum nigrum grown in different media for quality source material. | the epicoccum nigrum (en) extract used in allergy disorders exhibits batch-to-batch variations in protein composition and allergenic potency. in this study, the allergens of en grown in different media were investigated. | 2000 | 10753019 |
| evaluation of different pcr-based dna fingerprinting techniques for assessing the genetic variability of isolates of the fungus epicoccum nigrum. | thirty-six strains of the fungus epicoccum nigrum, isolated from different substrata and ecosystems of europe, america and africa, were analysed using 14 molecular markers included in 5 different genetic fingerprinting techniques: ap-pcr, tdna-pcr, microsatellite-primed pcr, ardra and aflp. all of the techniques used were able to differentiate the isolates, showing a high genetic diversity within the species. however, the different techniques detected different levels of similarity among the str ... | 1999 | 10692074 |
| ribosomal dna internal transcribed spacer sequences do not support the species status of ampelomyces quisqualis, a hyperparasite of powdery mildew fungi. | phylogenetic relationships among ampelomyces isolates, pycnidial hyperparasites and biological control agents of powdery mildews, were inferred from internal transcribed spacer (its) sequences of the ribosomal dna (rdna). currently, these hyperparasites are considered to be a single species, a. quisqualis, despite observed morphological and cultural differences. ten ampelomyces isolates, representing seven previously defined its rflp groups, were sequenced and analyzed. sequence-divergence value ... | 1998 | 9618587 |
| microbial oxidative metabolism of diclofenac: production of 4'-hydroxydiclofenac using epiccocum nigrum imi354292. | the 4'-hydroxylated metabolite of diclofenac was produced by biocatalysis for probing specific human drug-metabolising enzymes (cyp2c9). an initial screen of 11 microorganisms was carried out (50 ml scale) to identify the organism best suited to the regioselective conversion of diclofenac to its 4'-hydroxylated metabolite. from this screen, the fungus epicoccum nigrum imi354292 was selected as the most suitable microorganism. scale-up was carried out in a 30-l fermenter to which 2 g diclofenac w ... | 1998 | 9615477 |
| allergic fungal sinusitis in the southeastern usa: involvement of a new agent epicoccum nigrum ehrenb. ex schlecht. 1824. | alternaria alternata, aspergillus spp., bipolaris spicifera, curvularia lunata, epicoccum nigrum and fusarium solani were isolated repeatedly from groups of patients among 96 diagnosed with allergic fungal sinusitis (afs). epicoccum nigrum was obtained consistently from four patients, one of whom yielded mycelial masses consistent in morphology with e. nigrum. fifteen of the predominant fungi recovered from air samples from selected patients' residences included the same species isolated from th ... | 1997 | 9467107 |
| mold spectrum of four cereal brands of the german crop 1995. | the concentration of extracellular polysaccharides (eps) of 80 cereal grain samples of the german harvest 1995 was investigated. in addition to this, the total as well as the inherent mycoflora of two winter wheat and two winter rye brands which showed the highest eps-concentration was detected on maltsalt agar (nacl 5%). surface disinfection with 1% naocl for the determination of the internal mycoflora caused a significant reduction in number and kind of fungi. however, the mycoflora of all sam ... | 1997 | 9232848 |
| enumeration of fungi in barley. | estimation of fungal contamination of barley grain is important as certain fungi can proliferate during the malting process. the following factors which may affect the enumeration of fungi were evaluated: dilution versus direct plating, presoaked versus unsoaked grain, five culture media: potato dextrose agar (pda), acidified czapek-dox agar (aca), pentachloronitrobenzene agar; (pcnb) dichloran rose bengal chloramphenicol agar (drbc) and malt salt agar; two disinfectants' ethanol/water (80:20 v/ ... | 1997 | 9105919 |
| basement shower hypersensitivity pneumonitis secondary to epicoccum nigrum. | two children developed hypersensitivity pneumonitis after extensive exposure to an unventilated basement shower. commercial precipitin panels were negative. after home inspection, individual mold species were isolated from the household and extracted. precipitating antibodies to epicoccum nigrum were found in both children. resolution of the hypersensitivity pneumonitis occurred with avoidance and glucocorticosteroid therapy. e nigrum is a newly identified etiologic agent for hypersensitivity pn ... | 1996 | 8797443 |
| structure of triornicin, a new siderophore. | spectroscopic analysis of the tumor inhibitory factor triornicin produced by epicoccum purpurascens indicated that it was of similar structure to the known siderophore desferricoprogen, which is also produced by the fungus. the 1h and 13c nmr spectra indicated the replacement of an (e)-5-hydroxy-3-methyl-2-pentenoyl moiety of the desferricoprogen structure with an acetyl function. cleavage of triornicin with basic methanol produced two fragments. the first was identified as a natural siderophore ... | 1981 | 7195281 |
| aeroallergens of the southeast missouri area: a report of skin test frequencies and air sampling data. | aeroallergens in the southeast missouri area were identified by analyzing the positive skin tests of eight hundred seventeen patients and five years of air sampling. the important trees, grasses and weeds are enumerated. reactivity levels of house dust, feathers and dermatophagoides farinae exceeded all others. first among the fungi was epicoccum purpurascens . | 1984 | 6731948 |