Publications

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valorization of bakery waste for biocolorant and enzyme production by monascus purpureus.a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ...201627208753
the mechanism of binding of glucoamylase i from aspergillus awamori var. kawachi to cyclodextrins and raw starch.glucoamylase i (gai) bound to β-cyclodextrin (β-cd) with the binding constants of kd = 17.7 μm and n = 1.69. binding resulted in formation of an inclusion complex, as indicated by the fact that organic guest compounds for β-cd inhibited the binding of gai to β-cd. titration of gai (a(1)-r(615)) with β-cd or amylose a showed critical spectral perturbations at 286 nm that reflected the aromatic side chains of tyr and trp, but no spectral changes were observed in gai' (a(1)-v(469)) and gp-i (a(470) ...199427315704
transxylosylation of β-xylosidase from aspergillus awamori k4.β-xylosidase from aspergillus awamori k4 was purified. the optimum ph and temperature were around ph 4 and 70°c, and the molecular weight was estimated to be 117,000 on sds-page analysis. the enzyme has broad acceptor specificity in transxylosylation. especially, its acceptor accessibility for sorbitol and mannitol of sugar alcohols were higher than that for monosaccharides. trehalose was a much more effective acceptor than maltose and lactose of other disaccharides. in the reaction with 13-14% ...199727396877
three different types of α-amylases from aspergillus awamori kt-11: their purifications, properties, and specificities.three forms of α-amylases, designated amyl i, amyl ii, and amyl iii were purified to a homogenous state by several column chromatographies from a koji culture in wheat bran of a strain of black mold, which was isolated in indonesia and identified as aspergillus awamori. they have molecular weights of 49,000, 63,000, and 97,000 by sds-page, respectively, and the optimum phs were 4.0 for amyl i and 5.5 for both amyl ii and amyl iii on soluble starch. amyl i hydrolyzed malto-tetraose, -pentaose, -h ...199827396998
species distribution and in vitro azole susceptibility of aspergillus section nigri isolates from clinical and environmental settings.aspergillus section nigri includes species of interest for animal and human health, although studies on species distribution are limited to human cases. data on the antifungal susceptibilities and the molecular mechanism of triazole resistance in strains belonging to this section are scant. forty-two black aspergillus strains from human patients (16 isolates), animals (14 isolates), and the environment (12 isolates) were molecularly characterized and their in vitro triazole susceptibilities inve ...201627413191
biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate.waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ...201627416509
transformation of litchi pericarp-derived condensed tannin with aspergillus awamori.condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ...201627420043
studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil.in this study, use of inorganic fertilizer (n.p.k) was compared with organic manure (compost) in the bioremediation of diesel-polluted agricultural soil over a two-month period. renewal by enhanced natural attenuation was used as control. the results revealed that total petroleum hydrocarbon removal from polluted soil was 71.40 ± 5.60% and 93.31 ± 3.60% for n.p.k and compost amended options, respectively. the control (natural attenuation) had 57.90 ± 3.98% of total petroleum hydrocarbon removed. ...201627494315
determination and optimization of vitamin b complex in xylanase enzyme treated polished rice by response surface methodology.the present study provides information about the concentrations of vitamin b (thiamine, riboflavin, pyridoxine and niacin) in polished brown rice treated with xylanase. xylanase enzyme was produced from aspergillus awamori mtcc 9166. brown rice was treated with 60-100% enzyme (40 ml of buffer -undiluted) for 30 to 150 min (with variation of 30 min) at 30 degrees c to 50 degrees c (with variation of 5 degrees c) to attain a saturated moisture level of 35.5 g100(-1)g .the enzyme acted upon selecti ...201627498499
aspergillus awamori-fermented mung bean seed coats enhance the antioxidant and immune responses of weaned pigs.the potential benefits of aspergillus-fermented mung bean seed coats (fmsc) for weaned pigs remain unexplored. both in vitro and in vivo experiments were employed to evaluate the potential of fmsc supplement on the growth, antioxidant and immune responses of weaned pigs. the total polyphenols and dpph scavenging capability of ethanol extract of fmsc exhibited a greater (p < 0.01) increase than those of pre-fermentation. with the addition of the polyphenol of fmsc extract, an increase in phagocyt ...201728063238
immobilization of halophilic aspergillus awamori em66 exochitinase on grafted k-carrageenan-alginate beads.a novel extreme halophilic exochitinase enzyme was produced by honey isolate aspergillus awamori em66. the enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (ca) with 93 % immobilization yield. the immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. the significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. distinguishable characteristics of o ...201628330097
optimization of d-lactic acid production using unutilized biomass as substrates by multiple parallel fermentation.this study investigated the optimization of d-lactic acid production from unutilized biomass, specifically banana peel and corncob by multiple parallel fermentation (mpf) with leuconostoc mesenteroides and aspergillus awamori. the factors involved in mpf that were assessed in this study comprised banana peel and corncob, kh2po4, tween 80, mgso4·7h2o, nacl, yeast extract, and diammonium hydrogen citrate to identify the optimal concentration for d-lactic acid production. optimization of these comp ...201628330258
production, immobilization and thermodynamic studies of free and immobilized aspergillus awamori amylase.enzyme cost, stability and its thermodynamic characteristics are the main criteria for industrial use. in this study, aspergillus awamori amylase was constitutively produced using various agro-industrial wastes. olive oil cake gave the highest activity (230u/g). the amylase was partially purified to 2.81-fold purification. immobilization was achieved using different carriers by covalent binding. the novel carrier ca(+2) alginate (alg) starch (st)/polyethyleneimine (pei)/glutaraldehyde (ga), show ...201728438682
enhanced biodiesel production through phyco-myco co-cultivation of chlorella minutissima and aspergillus awamori: an integrated approach.algae-fungus co-culture was investigated as an alternative biodiesel feedstock. an oleaginous filamentous fungus aspergillus awamori was co-cultured with chlorella minutissima mcc 27 and chlorella minutissima utex 2219, respectively in n11 medium furnished with different carbon and nitrogen sources. the biomass and lipid production potential of the two c. minutissima-a. awamori co-cultures was compared against the monocultures. a substantial enhancement in biomass and lipid accumulation was obse ...201728475992
biochemical characterization of the xylan hydrolysis profile of the extracellular endo-xylanase from geobacillus thermodenitrificans t12.endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. hemicellulose degradation by geobacillus spp. is facilitated by the hemicellulose utilization (hus) locus that is present in most strains belonging to this genus. as part of the hus locus, the xyna gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. several geobacillus endo-xylanases have been charact ...201728521816
the role of soybean extracts and isoflavones in hormone-dependent breast cancer: aromatase activity and biological effects.estrogen receptor-positive (er(+)) breast cancer is the most common cause of cancer death in women worldwide. nowadays, the relationship between soya diet and breast cancer is controversial due to the unknown role of its isoflavones, genistein (g) and daidzein (d). in this work, we investigated not only the anti-tumor properties of a soybean extract (nbse) but also whether the biotransformation of extract (bse) by the fungus aspergillus awamori increased its effectiveness. the bse showed a stron ...201728644496
bioactive profile, free-radical scavenging potential, dna damage protection activity, and mycochemicals in aspergillus awamori (mtcc 548) extracts: a novel report on filamentous fungi.biomass of aspergillus awamori was investigated for mycochemicals, total phenolic compounds (tpc), condensed tannin content (ctc), free-radical scavenging potential (frsp), and dna damage protection activity. frsp was determined using dpph, abts, frap (ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. water (aq), aqueous ethanol 50% (aqe), and methanol were used as extraction phase at 44.5 °c for 23.8 min. aqe shows the presence of ma ...201728660460
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