Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| roles of extracellular matrix components in tiger frog virus attachment to fathead minnow (pimephales promelas) cells. | tiger frog virus (tfv) belongs to the genus ranavirus (family iridoviridae) and causes significant harm in cultured frogs, resulting in substantial losses in ecological and economic field in southern china. attachment is the first step in viral life cycle, which is dependent on the interactions of virions with extracellular matrix (ecm) components. studying this process will help in understanding virus infection and controlling viral diseases. in this study, the roles of primary ecm components i ... | 2020 | 32976972 |
| rapid diagnosis of largemouth bass ranavirus in fish samples using the loop-mediated isothermal amplification method. | largemouth bass ranavirus (lmbv) has been recognized as the causative pathogen responsible for infectious skin ulcerative syndrome in cultured largemouth bass in china. a fast and simple lmbv detection method is urgently needed. here, a loop-mediated isothermal amplification (lamp) assay was established for the detection of this virus using primers targeting the major capsid protein gene of lmbv. the amplification conditions were optimized; the assay was specific for the diagnosis of lmbv, as th ... | 2020 | 32268179 |
| effect of chlorine dioxide and phosphates on the quality of tiger frog (rana tigrina) meat during 4 °c storage. | tiger frog (rana tigrina) meat is extremely perishable. this study investigated the antimicrobial efficacy of chlorine dioxide (clo2 ) on frog meat, optimized the formulation of a phosphate-based enhancement solution by response surface methodology (rsm), and determined the quality parameters (i.e., total aerobic counts [tac], ph, drip loss, cooking loss, color measurements, shear force, total volatile basic nitrogen [tvb-n], and thiobarbituric acid-reactive substances [tbars]) of refrigerated f ... | 2020 | 32249422 |
| the combined effects of temperature and aromatase inhibitor on metamorphosis, growth, locomotion, and sex ratio of tiger frog (hoplobatrachus rugulosus) tadpoles. | the tiger frog (hoplobatrachus rugulosus) is widely raised by many farms in southern region of china as an economically edible frog. the growth, development, and sexual differentiation of amphibians are influenced by temperature and steroid hormone level. however, the problem of hormone residues is caused by the addition of exogenous hormones in frog breeding, it is worth considering whether non-sterol aromatase inhibitors can be used instead of hormones. | 2020 | 32219039 |
| asian bullfrog (rana tigerina) skin gelatin extracted by ultrasound-assisted process: characteristics and in-vitro cytotoxicity. | impact of ultrasound-assisted process (uap) on yield and characteristics of asian bullfrog (rana tigerina) skin gelatin was studied and the in-vitro cytotoxicity of the resulting frog skin gelatin was evaluated using caco-2, raw264.7 and l929 cell lines. different ultrasonication modes (5 min on-time with 25 min off-time (5/25) and 10 min on-time with 50 min off-times (10/50)) were implemented for different total ultrasonication times (10-60 min) during gelatin extraction at 55 °c. uap could eff ... | 2020 | 31954782 |
| the use of fish and herptiles in traditional folk therapies in three districts of chenab riverine area in punjab, pakistan. | like botanical taxa, various species of animals are also used in traditional and modern health care systems. present study was intended with the aim to document the traditional uses of herptile and fish species among the local communities in the vicinity of the river chenab, punjab pakistan. | 2020 | 32580733 |
| studies on the life cycle of pleurogenoides wayanadensis shinad & prasadan, 2018 (digenea: pleurogenidae) from the western ghats, india. | the life cycle of pleurogenoides wayanadensis shinad & prasadan, 2018, infecting the frogs hoplobatrachus tigerinus and euphlyctis cyanophlyctis, is elucidated in this study. all the life cycle stages from egg to egg-producing adults were elucidated under natural conditions and successfully established in the laboratory. the life cycle took about 58 to 65 days for completion. miracidia were released by teasing the eggs with fine needles. sporocysts were found in the freshwater snail, bithynia (d ... | 2020 | 32985400 |
| microbial community structures and important associations between soil nutrients and the responses of specific taxa to rice-frog cultivation. | rice-frog cultivation is a traditional farming system in china and has been reintroduced as an agricultural practice in china in recent years. the microbial community in paddy rhizospheric soils has attracted much attention because many microorganisms participate in functional processes in soils. in this study, illumina miseq sequencing-based techniques were used to investigate soil microbial communities and functional gene patterns across samples obtained by conventional rice cultivation (cr) a ... | 2019 | 31440215 |
| tiger frog virus orf104r interacts with cellular vdac2 to inhibit cell apoptosis. | ranaviruses belong to the family iridoviridae, and have become a serious threat to both farmed and natural populations of fish and amphibians. previous reports showed that ranaviruses could encode viral bcl-2 family-like proteins (vbcl-2), which play a critical role in the regulation of cell apoptosis. however, the mechanism of ranaviruses vbcl-2 interactions with host protein in mediating apoptosis remains unknown. tiger frog virus (tfv) belonging to the genus ranavirus has been isolated from i ... | 2019 | 31299465 |
| development and characterization of 33 microsatellite loci for the tiger frog hoplobatrachus rugulosus (wiegmann 1834) through transcriptome sequencing. | the tiger frog hoplobatrachus rugulosus (wiegmann 1834) is a large robust dicroglossid frog widely distributed in southern china, malaysia, myanmar, vietnam and thailand. the escaped bred tiger frog introduced from thailand hybridized with chinese native population may have affected the genetic diversity of local chinese tiger frogs. however, previous microsatellite loci of this species do not offer enough information to construct the genetic map. here, we reported 33 new microsatellite loci fro ... | 2018 | 30574879 |
| protein a from orange-spotted grouper nervous necrosis virus triggers type i interferon production in fish cell. | family nodaviridae consists of two genera: alphanodavirus and betanodavirus, and the latter is classified into four genotypes, including red-spotted grouper nervous necrosis virus, tiger puffer nervous necrosis virus, striped jack nervous necrosis virus, and barfin flounder nervous necrosis virus. type i interferons (ifns) play a central role in the innate immune system and antiviral responses, and the interactions between ifn and nnv have been investigated in this study. we have found that the ... | 2018 | 29733958 |
| two new species of pleurogenoides (luhe, 1901) travassos, 1921 (digenea: pleurogenidae) infecting two species of frogs of the western ghats, india. | two new species of digenetic trematodes of the genus pleurogenoides infecting the freshwater frogs, euphlyctis cyanophlyctis and hoplobatrachus tigerinus inhabiting the western ghats, india are described and illustrated. pleurogenoides euphlycti n. sp. and p. wayanadensis n. sp. are separated from their congeners on the basis of differences in the morphology and morphometry. the prevalence of infection of p. euphlycti n. sp. in e. cyanophlyctis is 8.99% and in h. tigerinus 12.5% and the intensit ... | 2018 | 29491572 |
| cosmocerca bengalensis sp. nov. (nematoda: cosmocercidae) in hoplobatrachus tigerinus (daudin, 1803) (amphibia, anura, dicroglossidae) from west bengal, india. | cosmocerca bengalensis sp. nov. (ascaridida, cosmocercidae) recovered from the rectum of an indian bullfrog, hoplobatrachus tigerinus (daudin, 1803), collected from hetampur town in birbhum district of west bengal, india, is described and illustrated. this species is similar to c. acanthurum, c. banyulensis, c. cruzi, c. japonica, c. kalesari, c. microhylae, c. novaeguineae, c. ornata, c. paraguayensis, c. parva, c. podicipinus and c. travassosi in having 5 pairs of plectanes supporting preanal ... | 2018 | 30367756 |
| what's for dinner? diet and potential trophic impact of an invasive anuran hoplobatrachus tigerinus on the andaman archipelago. | amphibian invasions have considerable detrimental impacts on recipient ecosystems. however, reliable risk analysis of invasive amphibians still requires research on more non-native amphibian species. an invasive population of the indian bullfrog, hoplobatrachus tigerinus, is currently spreading on the andaman archipelago and may have significant trophic impacts on native anurans through competition and predation. we carried out diet analyses of the invasive h. tigerinus and native anurans, acros ... | 2018 | 30310745 |
| first report of seuratascaris numidica seurat, 1917 (nematoda: ascarididae) and other helminths from amphibians of west bengal, india. | studies on nematode parasites from anuran hosts from eastern india, particularly west bengal, are rare. to our knowledge, there is a report of larvae of monhysterides sp. (a fish parasite) from cysts within the body cavity and musculature of duttaphrynus melanostictus, hoplobatrachus tigerinus and euphlyctis hexadactylus as well as descriptions of aplectana duttaphryni from d. melanostictus, aplectana dubrajpuri from h. tigerinus, cosmocerca microhylae from microhyla rubra and rhabdias bulbicaud ... | 2017 | 28316428 |
| inter- and intra-specific genetic divergence of asian tiger frogs (genus hoplobatrachus), with special reference to the population structure of h. tigerinus in bangladesh. | the five frog species of the genus hoplobatrachus are widely distributed in asia and africa, with asia being considered the genus' origin. however, the evolutionary relationships of asian hoplobatrachus species remain ambiguous. additionally, genetic diversity and fundamental differentiation processes within species have not been studied. we conducted molecular phylogenetic analysis on asian hoplobatrachus frogs and population genetic analysis on h. tigerinus in bangladesh using the mitochondria ... | 2017 | 27452042 |
| growth potential of rana tigerina skin lipids in cell cultures. | this study involves the investigation of the lipid composition of the skin of rana tigerina which has a significant healing capacity. the results indicated that the lipid extract enhanced keratinocyte and fibroblast cell proliferation progressively and were found to be much more efficient in comparison to agents known to cause cell proliferation and to be anti-inflammatory such as hydrocortisone. cell proliferation was dose dependent and suppression occurred only at very high doses. [3h]thymidin ... | 2017 | 9719416 |
| differential autophagic effects triggered by five different vertebrate iridoviruses in a common, highly permissive mandarinfish fry (mff-1) cell model. | autophagy of five vertebrate iridoviruses, including one megalocytivirus (infectious spleen and kidney necrosis virus, isknv) and four ranaviruses (chinese giant salamander iridovirus, cgsiv; tiger frog virus, tfv; grouper iridovirus, giv; and largemouth bass virus, lmbv) were investigated in a common, highly permissive mandarinfish fry (mff-1) cell model. the results showed marked autophagosome formation in giv- and lmbv-infected cells but not in isknv-, cgsiv- and tfv-infected mff-1 cells. str ... | 2016 | 26748344 |
| beta-glucuronidase in kidney-adrenal of male frogs (rana tigrina): a correlation of observations on histochemistry and biochemistry. | 2016 | 4993430 | |
| prevalence of spirometra mansoni in dogs, cats, and frogs and its medical relevance in guangzhou, china. | sparganosis is an important parasitic disease in guangzhou and is mainly acquired through the consumption of frog meat or contact with fresh frogs infected by larval stages (spargana) of the tapeworm species spirometra mansoni. | 2016 | 27777093 |
| budding of tiger frog virus (an iridovirus) from hepg2 cells via three ways recruits the escrt pathway. | the cellular endosomal sorting complex required for transport (escrt) pathway is a multifunctional pathway involved in cell physiological activities. while the majority of rna viruses bearing l-domains are known to hijack the escrt pathway to complete the budding process, the budding of large and complex enveloped dna viruses, especially iridoviruses, has been rarely investigated. in the present study, we use the tiger frog virus (tfv) as a model to investigate whether iridoviruses are released ... | 2016 | 27225426 |
| identification and differential expression analysis of micrornas encoded by tiger frog virus in cross-species infection in vitro. | tiger frog virus (tfv), dsdna virus of the genus ranavirus and family iridoviridae, causes a high mortality of tiger frog tadpoles cultured in southern china. micrornas (mirnas) have been identified in many viruses especially dna viruses such as singapore grouper iridoviruses (sgiv). micrornas play important roles in regulating gene expression for virus subsistence in host. considering that tfv infects cells of different species under laboratory conditions, we aim to identify the specific and es ... | 2016 | 27129448 |
| tiger frog virus orf080l protein interacts with litaf and impairs egf-induced egfr degradation. | tiger frog virus (tfv) belongs to the genus ranavirus, family iridoviridae, and causes severe mortality in commercial cultures in china. tfv orf080l is a gene homolog of lipopolysaccharide-induced tnf-α factor (litaf), which is a regulator in endosome-to-lysosome trafficking through its function in the endosomal sorting complex required for transport machinery. the characteristics and biological roles of tfv orf080l were identified. tfv orf080l was predicted to encode an 84-amino acid peptide (v ... | 2016 | 26956473 |
| caveolae restrict tiger frog virus release in hepg2 cells and caveolae-associated proteins incorporated into virus particles. | caveolae are flask-shaped invaginations of the plasma membrane. caveolae play important roles in the process of viruses entry into host cells, but the roles of caveolae at the late stage of virus infection were not completely understood. tiger frog virus (tfv) has been isolated from the diseased tadpoles of the frog, rana tigrina rugulosa, and causes high mortality of tiger frog tadpoles cultured in southern china. in the present study, the roles of caveolae at the late stage of tfv infection we ... | 2016 | 26887868 |
| predation by asian bullfrog tadpoles, hoplobatrachus tigerinus, against the dengue vector, aedes aegypti, in an aquatic environment treated with mosquitocidal nanoparticles. | aedes aegypti is a primary vector of dengue and chikungunya. the use of synthetic insecticides to control aedes populations often leads to high operational costs and adverse non-target effects. botanical extracts have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles, but their impact against predators of mosquito larvae has not been well studied. we propose a single-step method for the biosynthesis of silver nanoparticles (agnp) using the extract of artemisia vulgar ... | 2015 | 26091763 |
| spontaneous occurrence of aneuploidy in somatic cells of rana tigrina (ranidae: anura). | 2015 | 4547306 | |
| aplectana dubrajpuri sp. nov. (nematoda: cosmocercidae) in hoplobatrachus tigerinus (anura: dicroglossidae) from dubrajpur, birbhum, west bengal, india. | aplectana dubrajpuri sp. nov., recovered from the rectum of indian bull frog, hoplobatrachus tigerinus, collected from dubrajpur in the birbhum district of west bengal, india, is described and illustrated. this species is characterised by absence of gubernaculum and differs from other species of aplectana which lack a gubernaculum (viz. a. akhrami, a. artigasi, a. chilensis, a. crossodactyli, a. crucifer, a. delirae, a. meridionalis, a. papillifera, a. praeputialis, a. tarija and a. vercammeni) ... | 2015 | 26204179 |
| do cryptic species exist in hoplobatrachus rugulosus? an examination using four nuclear genes, the cyt b gene and the complete mt genome. | he chinese tiger frog hoplobatrachus rugulosus is widely distributed in southern china, malaysia, myanmar, thailand, and vietnam. it is listed in appendix ii of cites as the only class ii nationally-protected frog in china. the bred tiger frog known as the thailand tiger frog, is also identified as h. rugulosus. our analysis of the cyt b gene showed high genetic divergence (13.8%) between wild and bred samples of tiger frog. unexpected genetic divergence of the complete mt genome (14.0%) was als ... | 2015 | 25875761 |
| frog intestinal perfusion to evaluate drug permeability: application to p-gp and cyp3a4 substrates. | to evaluate the reliability of using in situ frog intestinal perfusion technique for permeability assessment of carrier transported drugs which are also substrates for cyp enzymes. single pass intestinal perfusion (spip) studies were performed in frogs of the species rana tigrina using established method for rats with some modifications after inducing anesthesia. effective permeability coefficient (p eff) of losartan and midazolam was calculated in the presence and absence of inhibitors using th ... | 2015 | 26236236 |
| assessment of heavy metals and metalloids in tissues of two frog species: rana tigrina and euphlyctis cyanophlyctis from industrial city sialkot, pakistan. | in the present study, we investigated the concentrations of ni, fe, pb, cu, co, zn, cd, mn, and cr in selected body tissues (liver, stomach, kidney, heart, lungs, and skeletal muscles) of two frog species: rana tigrina and euphlyctis cyanophlyctis captured from industrial wastewater of sialkot city known worldwide for its tanning industry. the both frog species had darker appearance, distinctively different wet body weight, and snout-vent length. the results revealed that the heavy metal concent ... | 2015 | 25966879 |
| development and characterization of 27 new microsatellite markers for the indian bullfrog hoplobatrachus tigerinus and its congeneric species. | the indian bullfrog hoplobatrachus tigerinus and its four congeneric species are common frog species and distributed throughout south asia. due to recent human activity, they are facing a changing environment and reduction in natural population size. for effective conservation and molecular ecological studies, we therefore isolated and characterized microsatellite loci for these frogs. we obtained genomic data using an ion torrent pgm sequencer and designed 54 primer sets for candidate loci. by ... | 2014 | 25475937 |
| interaction of acetylcholine and caffeine on the isolated rectus abdominis of frog (rana tigrina). | 2014 | 151667 | |
| effect of the essential oil (e.o.) of psoralea corylifolia (linn) (p.c.) on isolated rectus abdominis of frog (rana tigrina). | 2014 | 407154 | |
| effects of co60 gamma radiations on the conduction properties of frog sciatic nerves. | the effect of co60 gamma radiations on the amplitude, detection period and duration of the action potential of sciatic nerves of rana tigrina frogs have been studied. dose levels of up to 120 kr were employed. irradiation of the nerves with dose levels upto 30 kr does not seem to affect the action potential profiles. the amplitude falls rapidly therafter, registering less than 5% of the orginal value after irradiation by a dose of 120 kr. qualitatively similar behaviour is seen in the case of de ... | 2014 | 1222995 |
| acute and joint toxicity of three agrochemicals to chinese tiger frog (hoplobatrachus chinensis) tadpoles. | we studied acute and joint toxicity of three different agrochemicals (chlorantraniliprole, flubendiamide-abamectin and penoxsulam) to chinese tiger frog (hoplobatrachus chinensis) tadpoles with the method of stability water tests. results showed that the three agrochemicals increased tadpole mortality. for acute toxicity, the lc50 values after 24, 48 and 72 h of chlorantraniliprole, flubendiamide-abamectin and penoxsulam exposure were 5.37, 4.90 and 4.68 mg/l; 0.035, 0.025 and 0.021 mg/l; 1.74, ... | 2014 | 25017745 |
| effects of hydroperiod duration on developmental plasticity in tiger frog (hoplobatrachus chinensis) tadpoles. | while developmental plasticity can facilitate evolutionary diversification of organisms, the effects of water levels as an environmental pressure on tiger frogs remains unclear. this study clarifies the relationship by studying the responses of tiger frog (hoplobatrachus chinensis) tadpoles to simulated hydroperiods (i.e., constant low water levels, constant high water levels, increasing water levels, decreasing water levels, rapid changes in water levels and gradual fluctuations in water levels ... | 2014 | 24668655 |
| the life history of pleurogenoides malampuzhensis sp. nov. (digenea: pleurogenidae) from amphibious and aquatic hosts in kerala, india. | the life-cycle stages of pleurogenoides malampuzhensis sp. nov. infecting the indian bullfrog hoplobatrachus tigerinus (daudin) and the skipper frog euphlyctis cyanophlyctis (schneider) occurring in irrigation canals and paddy fields in malampuzha, which forms part of the district of palakkad, kerala, are described. the species is described, its systematic position discussed and compared with the related species, p. gastroporus (luhe, 1901) and p. orientalis (srivastava, 1934). the life-cycle st ... | 2014 | 23517639 |
| genome-wide analyses of proliferation-important genes of iridovirus-tiger frog virus by rnai. | tiger frog virus (tfv), a species of genus ranavirus in the family iridoviridae, is a nuclear cytoplasmic large dna virus that infects aquatic vertebrates such as tiger frog (rana tigrina rugulosa) and chinese soft-shelled turtle (trionyx sinensis). based on the available genome sequences of tfv, the well-developed rna interference (rnai) technique, and the reliable cell line for infection model, we decided to analyze the functional importance of all predicted genes. firstly, a relative quantita ... | 2014 | 24886972 |
| selected emerging diseases of amphibia. | this review summarizes the most recent updates on emerging infectious diseases of amphibia. a brief summary of batrachochytrium dendrobatidis history, epidemiology, pathogenesis, life cycle, diagnosis, treatment, and biosecurity is provided. ambystoma tigrinum virus, common midwife toad virus, frog virus 3, rana grylio virus, rana catesbeiana ranavirus, mahaffey road virus, rana esculenta virus, bohle iridovirus, and tiger frog virus ranaviruses are extensively reviewed. emerging bacterial patho ... | 2013 | 23642863 |
| prevalence and characterization of quinolone resistance in laribacter hongkongensis from grass carp and chinese tiger frog. | laribacter hongkongensis is a food-borne bacterium associated with community-acquired gastroenteritis and diarrhoea. quinolone resistance was recently reported in bacterial isolates from aquatic products, but the molecular mechanisms for resistance were still unknown. in this study, a total of 157 l. hongkongensis strains were isolated from grass carps (n = 443) and chinese tiger frogs (n = 171). twenty-one ciprofloxacin-resistant strains were analysed for mutations in quinolone resistance-deter ... | 2013 | 23906590 |
| [prey selection by tiger frog larvae (hoplobatrachus chinensis) of two sympatric anuran species' tadpoles]. | we examined the prey selection and behavioral responses of tiger frog hoplobatrachus chinensis larvae exposed to unpalatable and palatable sympatric prey tadpoles, bufo melanostictus and pelophylax nigromaculatus. we found that after a short exposure to the toxic toad tadpoles b. melanostictus, predators may learn to decrease going after unpalatable prey, subsequently it seems they may express short-term behavioral memory in order to avoid the toxic prey. in general, h. chinensis showed no prefe ... | 2013 | 23775997 |
| reserpine-resistant adrenergic mechanisms in the cardiovascular system of the frog (rana tigrina). | 2013 | 480982 | |
| adrenoceptors in the heart of rana tigrina. | 2013 | 4157019 | |
| studies on the life-cycle of ganeo tigrinus mehra & negi, 1928 (digenea). | the life-history stages of ganeo tigrinus mehra & negi, 1928 infecting the indian bull frog hoplobatrachus tigerinus (daudin) are described, those from cercaria to egg-producing adult having been established in the laboratory. non-virgulate xiphidiocercariae are released by the planorbid snail indoplanorbis exustus (deshayes). metacercariae occur in the haemocoel of dragonfly nymphs and become infective to the frog h. tigerinus within 15 days. the pre-patent period is 45 days. growth and develop ... | 2012 | 22488428 |
| 5-ht receptors as novel targets for optimizing pigmentary responses in dorsal skin melanophores of frog, hoplobatrachus tigerinus. | biochemical identification of 5-ht has revealed similar projection patterns across vertebrates. in cns, 5-ht regulates major physiological functions but its peripheral functions are still emerging. the pharmacology of 5-ht is mediated by a diverse range of receptors that trigger different responses. interestingly, 5-ht receptors have been detected in pigment cells indicating their role in skin pigmentation. hence, we investigated the role of this monoaminergic system in amphibian pigment cells, ... | 2012 | 21880033 |
| cryptic anuran biodiversity in bangladesh revealed by mitochondrial 16s rrna gene sequences. | to survey the diversity of anuran species in bangladesh, we compared mitochondrial 16s rrna gene sequences (approximately 1.4 kbp) from 107 bangladesh frog specimens. the results of genetic divergence and phylogenetic analyses incorporating data from related species revealed the occurrence of at least eight cryptic species. hoplobatrachus tigerinus from two districts diverged considerably, indicating the involvement of a cryptic species. two fejervarya sp. (large and medium types) and hylarana c ... | 2012 | 22379983 |
| acoustic characteristics of the tiger frog, hoplobatrachus rugulosus, during the breeding season. | we recorded and described the calls and acoustic characteristics of the male tiger frog, hoplobatrachus rugulosus, in an artificial pond during mating season. spectral and temporal call parameters, along with call intensity were analyzed. three harmonics were distinguishable from the spectrogram. four patterns of dominant frequency were found in calls produced late at night, i.e, 3 patterns in the first harmonic (located in 500 hz section, 700 hz section, and 800 hz section respectively) and 1 i ... | 2011 | 21842543 |
| cloning, characterization, and biological function analysis of the sidt2 gene from siniperca chuatsi. | the systemic rna interference defective protein (sid)-1 plays an important role in dsrna uptake in cells. we identified the scsidt2 gene from the mandarin fish (siniperca chuatsi), which is the first-studied sid-1 homolog in fish. scsidt2 mrna is 3380 bp long and contains a 2568-bp open reading frame that encodes an 855-amino-acid protein with an n-terminal signal peptide and ten putative transmembrane domains. tissue distribution profile in healthy fish and expression profiles of scsidt2 in inf ... | 2011 | 21334374 |
| tiger frog virus (an iridovirus) entry into hepg2 cells via a ph-dependent, atypical caveola-mediated endocytosis pathway. | tiger frog virus (tfv), in the genus ranavirus of the family iridoviridae, causes high mortality of cultured tiger frog tadpoles in china. to explore the cellular entry mechanism of tfv, hepg2 cells were treated with drugs that inhibit the main endocytic pathways. we observed that tfv entry was inhibited by nh(4)cl, chloroquine, and bafilomycin, which all can elevate the ph of acidic organelles. in contrast, tfv entry was not influenced by chlorpromazine or overexpression of a dominant-negative ... | 2011 | 21543502 |
| mediation of cholino-piperine like receptors by extracts of piper nigrum induces melanin dispersion in rana tigerina tadpole melanophores. | the present study was carried out to determine the effects of lyophilized dried fruit extracts of piper nigrum and pure piperine on the tadpole melanophores of frog rana tigerina which offer excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. the nature of specific cellular receptors present on the neuro-melanophore junction and their involvement in pigmentary responses has been explored. | 2011 | 21663558 |
| on the action and mechanism of withaferin-a from withania somnifera, a novel and potent melanin dispersing agent in frog melanophores. | the present work was carried out to determine the effects of lyophilized root extracts of withania somnifera along with pure withaferin-a, on the isolated skin melanophores of frog, rana tigerina which are disguised type of smooth muscle cells and offer excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. the lyophilized extract of w. somnifera and its active ingredient withaferin-a induced powerful dose-dependent physiologically significant mel ... | 2011 | 21848494 |
| absence of kin discrimination in cannibalistic anuran tadpoles of the frog hoplobatrachus tigerinus (daudin). | kin discrimination was tested in the cannibalistic h. tigerinus tadpoles to know whether cannibalism is selectively directed towards non-kin members or it is indiscriminate. the association choice tests were conducted using satiated as well as starved subjects with the assumption that they will associate near non-sibs rather than near sibs with the intention of preferentially cannibalizing them. however, test tadpoles, fed or starved showed a random association choice with sibs and non-sibs, as ... | 2011 | 21615061 |
| complete mitochondrial genomes and novel gene rearrangements in two dicroglossid frogs, hoplobatrachus tigerinus and euphlyctis hexadactylus, from bangladesh. | we determined the complete nucleotide sequences of mitochondrial (mt) genomes from two dicroglossid frogs, hoplobatrachus tigerinus (indian bullfrog) and euphlyctis hexadactylus (indian green frog). the genome sizes are 20462 bp in h. tigerinus and 20280 bp in e. hexadactylus. although both genomes encode the typical 37 mt genes, the following unique features are observed: 1) the nd5 genes are duplicated in h. tigerinus that have completely identical sequences, whereas duplicated nd5 genes in e. ... | 2010 | 21041980 |
| development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of lymphocystis disease virus. | a loop-mediated isothermal amplification (lamp) assay was developed for the detection of lymphocystis disease virus (lcdv). a set of five specific primers, two inner and two outer primers and a loop primer, were designed on the basis of the major capsid protein gene of lcdv. the reaction time and temperatures were optimized for 60 min at 63 degrees c, respectively. lamp amplification products were detected by a ladder-like appearance on agarose gel electrophoresis or a naked-eye inspection of a ... | 2010 | 19891986 |
| efficacy of frog skin lipids in wound healing. | background: frog skin has been sequentially and scientifically evaluated by our group for its wound healing efficiency. owing to the complex structure of skin, attempts were being made to analyse the role of individual constituents in different phases of healing. our earlier papers have shown the significance of frog skin not only in wound healing but also enhancing the proliferating activity of the epidermal and dermal cells which are instrumental for normal healing process. we also have identi ... | 2010 | 20637131 |
| arginase isoforms in frog and lizard tissues. | isoforms of arginase in the liver and kidney tissues of the ureotelic frog (rana tigerina) and uricotelic lizard (calotes versicolor) were fractionated by deae-cellulose chromatography (ph 8.3). four molecular forms, designated as a'1, a2, a3 and a4 based on the kcl concentration required for their elution from the ion-exchange column, were detected in lizard liver, while only two forms were found in lizard kidney (a3 and a4) and frog liver and kidney (a2 and a3). no major differences were found ... | 2010 | 21086749 |
| energetics of metamorphic climax in the pickerel frog (lithobates palustris). | anuran metamorphosis, the transition from aquatic larvae to terrestrial juveniles, is accompanied by significant morphological, physiological, and behavioral changes. timing of metamorphosis and final size, which can influence adult fitness, may depend on sufficient energy accumulated during the larval period to support metamorphosis. however, only two species of anurans have been examined for energetic costs of metamorphosis, rana tigrina and anaxyrus terrestris. based on these species, it has ... | 2009 | 19508896 |
| isolation of laribacter hongkongensis, a novel bacterium associated with gastroenteritis, from chinese tiger frog. | laribacter hongkongensis is a recently discovered novel bacterium associated with community-acquired gastroenteritis. although the bacterium has been isolated from freshwater fish and natural freshwater environments, it is not known if other freshwater animals could also be a source of l. hongkongensis. in a surveillance study on freshwater food animals (other than fish) in hong kong, l. hongkongensis was isolated from eight of 10 chinese tiger frogs (hoplobatrachus chinensis), a widespread frog ... | 2009 | 19033083 |
| first isolation and identification of elizabethkingia meningoseptica from cultured tiger frog, rana tigerina rugulosa. | elizabethkingia meningoseptica has been recognised as an occasional but serious opportunistic bacterial pathogen to human beings. recently, it was frequently isolated from tiger frog, rana tigerina rugulosa, with cataract disease, which is the most common disease of unknown aetiology of frogs in hainan, china. the purpose of this study was to identify and characterise the bacterial strains isolated from the recent outbreaks of cataract disease in farmed tiger frog in hainan, china, and to evalua ... | 2009 | 19327918 |
| tiger frog virus can infect zebrafish cells for studying up- or down-regulated genes by proteomics approach. | tiger frog virus (tfv), a member of the iridovirus family, causes high mortality of cultured tiger frog tadpoles in southern china. to better understand tfv infection and its interaction with host cells, zebrafish embryonic fibroblast (zf4) cells, a stable polyploid cell line with most clear genetic map, was used for our present study. our results showed that tfv caused typical lytic plaque forming cytopathic effect (cpe) and that various stages of viral proliferation were observed using electro ... | 2009 | 19409947 |
| complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of iridoviridae. | soft-shelled turtle iridovirus (stiv) is the causative agent of severe systemic diseases in cultured soft-shelled turtles (trionyx sinensis). to our knowledge, the only molecular information available on stiv mainly concerns the highly conserved stiv major capsid protein. the complete sequence of the stiv genome is not yet available. therefore, determining the genome sequence of stiv and providing a detailed bioinformatic analysis of its genome content and evolution status will facilitate furthe ... | 2009 | 19439104 |
| purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of fejervarya cancrivora. | while investigating the innate defense of brackish water-living amphibian and its comparison with freshwater-living amphibians, two novel 12-residue antimicrobial peptides were purified from the skin secretions of the crab-eating frog, fejervarya cancrivora which typically inhabits brackish water of mangrove forests of southeast asia. these two antimicrobial peptides, tigerinin-rc1 and -rc2 share significant structural similarity with tigerinins found in the skin of indian frog, hoplobatrachus t ... | 2009 | 19540421 |
| plasma membrane electron transport in frog blood vessels. | in an attempt to see if frog blood vessels possess a plasma membrane electron transport system, the postcaval vein and aorta isolated from rana tigrina were tested for their ability to reduce ferricyanide, methylene blue, and 2,6-dichloroindophenol. while the dyes remained unchanged, ferricyanide was reduced to ferrocyanide. this reduction was resistant to inhibition by cyanide and azide. heptane extraction or formalin fixation of the tissues markedly reduced the capability to reduce ferricyanid ... | 2009 | 20093738 |
| effect of progesterone administration on the distribution of oviductal carbohydrates in rana tigrina. | our aim has been to determine whether carbohydrate distribution in the oviducts of progesterone-treated animals is comparable with that of seasonal breeders in rana tigrina. like many other anurans, r. tigrina oviduct exhibits a short straight portion (pars recta, pr) at the beginning followed by a long, highly coiled portion (pars convoluta, pc). histologically, the oviduct of this species revealed some unique features, one of which was intense toluidine blue staining, specifically in the upper ... | 2009 | 19340461 |
| inherent rhythmicity and interstitial cells of cajal in a frog vein. | interstitial cells of cajal are responsible for rhythmic contractions of the musculature of the gastrointestinal tract and blood vessels.the existence of these cells and spontaneous rhythmicity were noticed in amphibian vein and the findings are reported in this paper.the postcaval vein was identified in the frog, rana tigrina and was perfused with amphibian ringer solution after isolation.contractile activity was recorded through a tension transducer connected to a polygraph.the isolated postca ... | 2008 | 19179763 |
| identification of two novel membrane proteins from the tiger frog virus (tfv). | the tiger frog virus (tfv) belongs to the genus ranavirus in the family iridoviridae, and its genome was completely sequenced in 2002. in order to better understand the viral structure and functional genes involved in infection and virus-host interactions, two candidate genes, orf001l and orf020r, were selected for our study. orf001l and orf020r were analyzed by genomic comparison and by using the tmhmm software. both genes were conserved in the genus ranavirus, may encode putative membrane prot ... | 2008 | 18538433 |
| development of an rt-pcr detection method for mud crab reovirus. | mud crab reovirus (mcrv) causes high mortality in the cultivated mud crab. to control better an outbreak of this virus, a rapid, specific and sensitive detection method based on rt-pcr was developed. the mcrv detection method was designed based on the one-step and two-step rt-pcr which resulted in the amplification of predicted products of 433 and 304 bp. the method is specific as no cross-reaction was observed between grass carp hemorrhage virus (gchv), white spot syndrome virus (wssv), tiger f ... | 2008 | 18572256 |
| a survey on the faunal diversity of savar upazila, dhaka, bangladesh. | a survey was conducted during january to december 2006 to assess the status of faunal diversity of savar upazila, dhaka, bangladesh. a total of 30 species of birds, 24 species of winter birds, 7 species of reptiles, 3 species of amphibians, 15 species of mammalians and 32 species of fishes were recorded. relative abundance of those species were determined. of the birds, house sparrow (passer domesticus) was abundant while blyth's kingfisher (alcedo hercules), rock eagle owl (bubo bengalensis), h ... | 2008 | 18817158 |
| ubiquitous lens alpha-, beta-, and gamma-crystallins accumulate in anuran cornea as corneal crystallins. | corneal epithelium is known to have high levels of some metabolic enzymes such as aldehyde dehydrogenase in mammals, gelsolin in zebrafish, and alpha-enolase in several species. analogous to lens crystallins, these enzymes and proteins are referred to as corneal crystallins, although their precise function is not established in any species. although it is known that after lentectomy, the outer cornea undergoes transdifferentiation to regenerate a lens only in anuran amphibians, major proteins ex ... | 2007 | 17452334 |
| efficacy of lagenidium giganteum metabolites on mosquito larvae with reference to nontarget organisms. | lagenidium giganteum is a water mold and an effective mosquito control agent with limited use due to poor survival and contamination during storage. invert extracellular metabolites of l. giganteum is easy to produce, long shelf life, and a potential candidate in tropical climates. this fungus was grown in pyg broth in the laboratory at 25 +/- 2 degrees c, and relative humidity was maintained at 75 +/- 5% for 15 +/- 2 days. filtration process of metabolites was done using whatman filter paper, c ... | 2007 | 17334944 |
| a broadly applicable method to characterize large dna viruses and adenoviruses based on the dna polymerase gene. | many viral pathogens are poorly characterized, are difficult to culture or reagents are lacking for confirmatory diagnoses. we have developed and tested a robust assay for detecting and characterizing large dna viruses and adenoviruses. the assay is based on the use of degenerate pcr to target a gene common to these viruses, the dna polymerase, and sequencing the products. | 2006 | 16608526 |
| gel filtration behaviour of arginase in frog liver and kidney extracts. | the molecular weight of arginase, determined by gel filtration of frog (rana tigerina) liver and kidney extracts, varied with the protein concentration. it increased from about 25-27 kda in dilute solutions to about 92-96 kda in concentrated extracts. in addition, an arginase with a molecular weight of 45-49 kda appeared at intermediate concentrations. the results suggest the probable aggregation of arginase monomers to yield the dimeric and tetrameric forms of the enzyme at high protein concent ... | 2006 | 7689359 |
| chromatin organization and basic nuclear proteins in the male germ cells of rana tigerina. | the process of chromatin condensation during spermiogenesis in rana tigerina is similar to the heterochromatization in somatic cells, where 30 nm fibers are coalesced together into a dense mass in spermatozoa without changing their initial size and nucleosomal organization. this conclusion was supported by the finding that the full set of core histones (h2a, h2b, h3, h4) are still present in sperm chromatin, but histone h1 is replaced by its variant, h1v. rabbit anti-sera were raised against his ... | 2005 | 15570620 |
| inhibition of reporter gene and iridovirus-tiger frog virus in fish cell by rna interference. | we describe the specific silencing of reporter gene lacz in fhm cells (muscle cells of fathead minnow, a fish cell line) by either expressing small hairpin rnas (shrnas) from plasmids or transfecting small interfering rnas (sirnas) transcribed in vitro. two types of dsrnas could inhibit reporter gene expression, and sirnas were more effective, while both of them worked very well in hela cells. sirnas were tested for silencing expression of the major capsid protein (mcp) encoded by tiger frog vir ... | 2005 | 15932766 |
| identification and characterization of a glucagon receptor from the goldfish carassius auratus: implications for the evolution of the ligand specificity of glucagon receptors in vertebrates. | the structural basis of ligand selectivity of g protein-coupled receptors for metabolic hormones has been an area of intense investigation, and yet it remains unresolved. one approach to delineating the mechanism of ligand-receptor interactions is to compare the ligand specificities of receptors expressed in species that emerged at different times within vertebrate evolution. in this paper we describe the isolation, functional, and phylogenetic characterization of the glucagon receptor from the ... | 2004 | 15033912 |
| effects of garlic (allium sativum) extract on the heart rate, rhythm and force of contraction in frog: a dose-dependent study. | garlic juice (dose equivalent to 3.3 g to 33 g garlic) mainly caused bradycardia in frog rana tigerina. the disturbance in ventricular rhythm was observed prior to than that of atria. rhythm was specially disturbed at higher doses causing bizarre pattern. force of contraction of the heart also decreased with higher dose of the garlic extract. the results suggest that garlic extract has some beneficial effect on heart rate modulating the rate, rhythm and force of contraction positively but very h ... | 2004 | 15260118 |
| genomic sequence of a ranavirus (family iridoviridae) associated with salamander mortalities in north america. | disease is among the suspected causes of amphibian population declines, and an iridovirus and a chytrid fungus are the primary pathogens associated with amphibian mortalities. ambystoma tigrinum virus (atv) and a closely related strain, regina ranavirus (rrv), are implicated in salamander die-offs in arizona and canada, respectively. we report the complete sequence of the atv genome and partial sequence of the rrv genome. sequence analysis of the atv/rrv genomes showed marked similarity to other ... | 2003 | 14599794 |
| effect of beta-cyclodextrin compounds on the solubilization of three selected pesticides and their toxicity with methyl parathion to rana tigrina tadpoles. | 2003 | 12719827 | |
| pesticide impact on excretory physiology of the common frog, rana tigrina (daud) tadpoles. | 2002 | 12068930 | |
| application of frog (rana tigerina daudin) skin collagen as a novel substrate in cell culture. | collagen and collagen-based materials have extensive application in biomedical devices and tissue engineering. the current paper pertains to the application of frog (rana tigerina daudin) skin collagen as a novel substrate in cell culture. the study deals with the behavior, morphology, and physiology of keratinocytes and fibroblasts over dry and reconstituted collagen substratum, which are the key cells involved in wound repair. the advantage of using frog skin collagen as a substratum lies in t ... | 2002 | 12007199 |
| sequence analysis of the complete genome of an iridovirus isolated from the tiger frog. | we have isolated a tiger frog virus (tfv) from diseased tiger frogs, rana tigrina rugulosa. the genome was a linear double-stranded dna of 105,057 basepairs in length with a base composition of 55.01% g+c. about 105 open reading frames were identified with coding capacities for polypeptides ranging from 40 to 1294 amino acids. computer-assisted analyses of the deduced amino acid sequences revealed that 39 of 105 putative gene products showed significant homology to functionally characterized pro ... | 2002 | 11878922 |
| the chemoarchitectonics of the diencephalon of frog (rana tigrina). | the study deals with the distribution of acid and alkaline phosphatases, atpase, 5-nucleotidase, nonspecific esterase, specific cholinesterase, and beta-galactosidase in the diencephalon of the frog. the highlights of the present study are the following: i) acid phosphatase is present in all the neurons, whereas the tracts and commissures are completely negative. ii) most of the tracts and commissures are positive for 5-nucleotidase. this confirms the author's previous findings that the tracts a ... | 2002 | 154581 |
| the effect of 17 beta-estradiol acetate on the testis and thumb pad of rana cyanophlyctis (schn.) and rana tigrina (daud.). | 2002 | 1222901 | |
| immunocytochemical localization of growth hormone-releasing hormone-like peptide in the brain of the tiger frog, rana tigrina. | using antisera directed against carp growth hormone-releasing hormone (cghrh), we found more extensive brain distribution of ghrh-like immunoreactive (ir) neurons in the tiger frog, rana tigrina, than reported in previous studies, which employed mammalian ghrh antibodies. in the telencephalon, ghrh-ir perikarya were present in the pallium dorsale, pallium laterale (pars dorsalis and pars ventralis), pallium mediale, nucleus entopeduncularis, amygdala pars medialis, and in the ventral portion of ... | 2002 | 12030776 |
| out of asia: mitochondrial dna evidence for an oriental origin of tiger frogs, genus hoplobatrachus. | most examples of intercontinental dispersal events after the miocene contact between africa and asia involve mammal lineages. among amphibians, a number of probably related groups are known from both continents, but their phylogenies are so far largely unresolved. to test the hypothesis of miocene dispersal against a mesozoic vicariance scenario in the context of gondwana fragmentation, we analyzed fragments of the mitochondrial 16s rrna gene (572 bp) in 40 specimens of 34 species of the anuran ... | 2001 | 11741382 |
| structural and functional identification of the pituitary adenylate cyclase-activating polypeptide receptor vpac2 from the frog rana tigrina rugulosa. | recently, a frog pituitary adenylate cyclase-activating polypeptide (pacap)/vasoactive intestinal peptide (vip) receptor (fpvr) has been characterized, and interestingly, this receptor exhibits characteristics of both mammalian pacap type ii receptors vpac(1)r and vpac(2)r. in order to investigate the receptors responsible for mediating the actions of vip and pacap in amphibians, in this report, a frog vpac(2) receptor (fvpac(2)r) cdna was isolated. fvpac(2)r shares 47.7, 46.9 and 62.5% amino ac ... | 2001 | 11564605 |
| identification of a proglucagon cdna from rana tigrina rugulosa that encodes two glp-1s and that is alternatively spliced in a tissue-specific manner. | glucagon plays a pivotal role in the regulation of metabolism. a glucagon receptor has been previously characterized in the frog, rana tigrina rugulosa, and the frog and human glucagon receptors have been shown to possess similar binding affinities toward human glucagon. to study the structural evolution of glucagon peptide and its receptor in vertebrates, in the current study, a proglucagon cdna from the same frog species was cloned. interestingly, in contrast to the mammalian proglucagons that ... | 2001 | 11703080 |
| studies on rana tigerina skin collagen. | the current paper pertains to the study of frog skin, more specifically rana tigerina skin collagen, which is a major extracellular matrix protein known to play an important role in the wound-healing process. this study revealed interesting differences in the frog skin collagen when compared to the hitherto known vertebrate collagens. this could probably be attributed to the position of the amphibians in the vertebrate hierarchy. therefore, detailed investigations on the various physico-chemical ... | 2001 | 11163307 |
| tigerinins: novel antimicrobial peptides from the indian frog rana tigerina. | four broad-spectrum, 11 and 12 residue, novel antimicrobial peptides have been isolated from the adrenaline-stimulated skin secretions of the indian frog rana tigerina. sequences of these peptides have been determined by automated edman degradation, by mass spectral analysis and confirmed by chemical synthesis. these peptides, which we have named as tigerinins, are characterized by an intramolecular disulfide bridge between two cysteine residues forming a nonapeptide ring. this feature is not fo ... | 2001 | 11031261 |
| short-term and long-term survival studies in rana tigrina tadpoles with reference to methyl parathion toxicity. | toxicity of methyl parathion was studied on survival of rana tigrina tadpoles. the lc50 values for short-term (upto 96 h) and long term studies (hatching to froglet) were determined. the lc50 values for 96 h and the total larval period were 4.36 and 1.30 ppm respectively. the toxicity of methyl parathion enchanced in relation to increase in concentration and duration. teratological defects such as scoliosis and failure in emergence of fore limb were noticed. safe levels were calculated for short ... | 2001 | 12018596 |
| interrelationship between food availability, fat body, and ovarian cycles in the frog, rana tigrina, with a discussion on the role of fat body in anuran reproduction. | long-term experiments were conducted to study the progression of vitellogenic cycles in rana tigrina (an annual breeder) having different foraging backgrounds and held under conditions of weekly or daily food supply and in presence or absence of abdominal fat bodies. they were autopsied in june to assess fecundity. in nature an adult r. tigrina produces on an average 4,000 eggs/100 g body mass (b.m.) and spawns in june-july following monsoon rains. weekly feeding from july to next breeding seaso ... | 2000 | 10684572 |
| virulence properties of motile aeromonads isolated from farmed frogs rana tigerina and r. rugulosa. | virulence factors were compared in aeromonas species isolated from clinically normal and septicaemic farmed frogs from thailand. haemolysin activities against frog erythrocytes were significantly different within the collection of aeromonads. groups of high haemolytic activity (unspeciated aeromonas, au), moderate haemolytic activity (a. hydrophila), and low haemolytic activity (a. veronii biovar sobria, a. veronii biovar veronii, a. caviae, a. schubertii) were noted. dna colony hybridisation st ... | 2000 | 10843556 |
| functional studies of a glucagon receptor isolated from frog rana tigrina rugulosa: implications on the molecular evolution of glucagon receptors in vertebrates. | in this report, the first amphibian glucagon receptor (glur) cdna was characterized from the liver of the frog rana tigrina rugulosa. functional expression of the frog glur in cho and cos-7 cells showed a high specificity of the receptor towards human glucagon with an ec(50) value of 0.8+/-0.5 nm. the binding of radioiodinated human glucagon to glur was displaced in a dose-dependent manner only with human glucagon and its antagonist (des-his(1)-[nle(9)-ala(11)-ala(16)]) with ic(50) values of 12. ... | 1999 | 10471837 |
| ultrastructure of spermatozoa correlated with phylogenetic relationship between heteropneustes fossilis and rana tigrina. | a study using transmission electron microscopy (tem) of the spermatozoa of rana tigrina and heteropneustes fossilis in all phases of the annual reproductive cycle revealed that there was a phylogenetic relationship between them. the spermatozoa of h. fossilis appeared horseshoe-shaped, somewhat oval or wedge-shaped at the anterior end and broader at the posterior end. the horseshoe-shaped spermatozoan nucleus was observed during spermiogenesis of r. tigrina but later changed into a finger shape ... | 1998 | 10093202 |
| histamine receptors in the skin melanophores of indian bullfrog rana tigerina. | histamine and 2-methyl histamine caused dose-dependent aggregation of the integumental melanophores of rana tigerina both in vitro and in vivo. the aggregating effects were antagonised by mepyramine and metiamide, specific h1 and h2 receptor blockers, respectively. compound 48/80 and edta augmented the melanin-aggregating effects of exogenously applied histamine and 2-methyl histamine in in vivo experiments. 4-methyl histamine, a specific h2 receptor agonist, dispersed the frog melanophores in i ... | 1998 | 9972321 |
| bioelectrical activity of brain in rana tigrina (daudin) in response to phosalone poisoning. | an acute dose of phosalone, intraperitoneally injected to rana tigrina alters the bioelectrical activity of the brain. the frequency levels of delta, theta, alpha, and beta waves remained similar in both control and experimental groups, while the amplitude of the waves was significantly decreased by 40 to 60% in the latter group. the total work done (twd) was also significantly reduced. the above disturbance in the brain electrical activity may have correlation to the inhibition of cation-linked ... | 1997 | 9440249 |
| light-induced changes in frog pineal gland n-acetyltransferase activity. | n-acetyltransferase (nat) activity was determined in the pineal gland of frogs (rana tigrina) of different ages using 2-aminofluorene and p-aminobenzoic acid as substrates, and assayed by high-pressure liquid chromatography. frogs of different ages were either killed during the light phase or exposed to darkness or light for 1 min during the dark phase of the lighting cycle, then returned to their cages in darkness for 30 min before being killed. the pineal gland nat activity of 1-month-old frog ... | 1997 | 9308002 |
| calcitonin-like immunoreactivity in the subcommissural organ and reissner's fiber in the teleost clarias batrachus, frog rana tigrina and lizard calotes versicolor. | in the cns of vertebrates, although the subcommissural organ (sco) has been identified as an ependymal brain gland and reissner's fiber (rf) as a condensed product of its secretion, the exact nature of the secretory substances has remained elusive. in the present study, immunocytochemical application of polyclonal antibodies against calcitonin revealed intense immunoreactivity in the cells, cell processes and cerebrospinal spinal fluid (csf)-contacting apical terminals of the columnar ependymal ... | 1997 | 9098563 |
| kinetics of acetyl coenzyme a:arylamine n-acetyltransferase from rapid and slow acetylator frog tissues. | n-acetyltransferase (nat) activity was determined in 100 frog (rana tigrina) livers using 2-aminofluorene and p-aminobenzoic acid as substrates. overall, the liver nat activity of the 50 females was higher than the liver nat activity of the 50 males. the activities (mean +/- sd) of nat from the bladder, blood, colon, and liver of males was 0.30 +/- 0.11, 0.05 +/- 0.03, 0.09 +/- 0.05, and 0.93 +/- 0.56 nmol/min/mg protein for the acetylation of aminofluorene and 0.29 +/- 0.06, 0.36 +/- 0.04, 0.26 ... | 1996 | 8742223 |
| phosalone poisoning on the cation-linked atpases of central nervous system of rana tigrina (daudin). | an acute dose of phosalone, an organophosphate pesticide, widely used in crop fields, injected to rana tigrina, intraperitoneally, inhibited the different inorganic ion linked atpase enzymes of the central nervous system (cns). six discrete cns compartments, namely telencephalon, mid-brain, rhombencephalon, cervical spinal cord, thoracic spinal cord and lumbar spinal cord showed markedly diminished atpase activity. atpases inhibition affected the neuronal activity and muscular coordination leadi ... | 1995 | 8564785 |