Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of processing on phenolics of wines. | phenolics of grapes are the main compounds responsible for color, taste, mouth feel, oxidation and other chemical reactions in wine and juice. phenolic levels in wine and juice are affected by numerous processing conditions (crushing, pressing, sulfite addition, skin contact, oak aging). studies were conducted to better understand the effect of processing on phenolic composition of three varieties of grapes. three different processing steps: immediate press, hot press, and hull treatment (skin c ... | 1998 | 9598211 |
| changes in the free amino acid composition with maturity of the noble cultivar of vitis rotundifolia michx. grape. | the changes in amino acid composition that occur with maturity of the noble cultivar of the vitis rotundifolia michx. (muscadine) grape were determined by hplc. eighteen amino acids were identified. histidine was the most prominent amino acid followed by alanine. the concentrations of most of the major amino acids (alanine, glycine, histidine, valine, isoleucine, aspartic acid, and serine) were highest at verasion. glutamine and threonine contents dropped sharply after fruit set, while those of ... | 1999 | 10606539 |
| wine modifies the effects of alcohol on immune cells of mice. | ethanol may be detrimental to immune cells due to the generation of free radicals during detoxification. if this is true, then alcoholic beverages that contain antioxidants, like red wine, should be protective against immune cell damage. we investigated this by giving mice either a red muscadine wine (vitis rotundifolia), a cabernet sauvignon (vitis vinifera), ethanol (all at 6% alcohol) or water in the water bottles as the sole fluid for 8 wk. plasma antioxidant capacity was measured with alpha ... | 2000 | 10801903 |
| ellagic acid and flavonoid antioxidant content of muscadine wine and juice. | antioxidant properties of flavonoids and ellagic acid were characterized in eight wines and juices produced by various processing methodologies from red and white muscadine grape cultivars (vitis rotundifolia). juices and wines were produced by hot- and cold-pressed techniques, and additional wine was produced following on-hull fermentation for 3, 5, and 7 days. chromatographic conditions were developed to simultaneously separate anthocyanins, ellagic acid, and flavonols and correlated to a meas ... | 2002 | 12009984 |
| ellagic acid and ellagitannins affect on sedimentation in muscadine juice and wine. | a mechanism for the formation of water-insoluble sediments in wines and juices made from red and white muscadine grapes (vitis rotundifolia) was investigated as a function of processing methodology and storage. sediments are considered quality defects in muscadine grape products, and their presence may influence consumer acceptability and expansion of retail markets. processing regimes included both hot (70 degrees c) and cold (25 degrees c) press techniques for wine or juice production, and fer ... | 2002 | 12083868 |
| optical properties of intact leaves for estimating chlorophyll concentration. | changes in leaf chlorophyll content can serve as relative indicators of plant vigor and environmental quality. this study identified reflectance, transmittance, and absorptance wavebands and band ratios within the 400- to 850-nm range for intact leaves that could be used to estimate extracted leaf chlorophyll per unit leaf area (areal concentration) with minimal error. leaf optical properties along with chlorophyll a, b, and a + b concentrations were measured for the planar-leaved sweetgum (liqu ... | 2002 | 12371158 |
| phytochemical stability and color retention of copigmented and processed muscadine grape juice. | muscadine (vitis rotundifolia) grape juice was assessed for color and phytochemical stability as influenced by anthocyanin copigmentation with a water-soluble rosemary extract, fortification with ascorbic acid, and processing by heat or high hydrostatic pressure (hhp). the roles of polyphenolic cofactors in the presence and in the absence of ascorbic acid were assessed as a means to improve the overall processing stability of the juice. addition of rosemary extract from 0 to 0.4% (v/v) readily f ... | 2003 | 12568555 |
| identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine. | oligonucleotide primers, designed to conserved regions of nucleotide binding site (nbs) motifs within previously cloned pathogen resistance genes, were used to amplify resistance gene analogs (rgas) from grapevine. twenty eight unique grapevine rga sequences were identified and subdivided into 22 groups on the basis of nucleic acid sequence-identity of approximately 70% or greater. representatives from each group were used in a bulked segregant analysis strategy to screen for restriction fragmen ... | 2002 | 12582665 |
| phenolic content and antioxidant capacity of muscadine grapes. | fruits of 10 cultivars of muscadine grapes (five bronze skin and five purple skin) grown in southern georgia were separated into skin, seed, and pulp. each fruit part and the leaves from the corresponding varieties were extracted for hplc analysis of major phenolics. total phenolics were determined colorimetrically using folin-ciocalteu reagent. total anthocyanins were determined according to a ph-differential method, using a uv-visible spectrophotometer. antioxidant capacity was determined by t ... | 2003 | 12926904 |
| major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid. | grape seeds and skins are good sources of phytochemicals such as gallic acid, catechin, and epicatechin and are suitable raw materials for the production of antioxidative dietary supplements. the differences in levels of the major monomeric flavanols and phenolic acids in seeds and skins from grapes of vitis vinifera varieties merlot and chardonnay and in seeds from grapes of vitis rotundifolia variety muscadine were determined, and the antioxidant activities of these components were assessed. t ... | 2004 | 14733505 |
| fruit maturity and juice extraction influences ellagic acid derivatives and other antioxidant polyphenolics in muscadine grapes. | polyphenolic compounds including ellagic acid, ellagic acid derivatives, and anthocyanins were characterized and quantified by novel chromatographic conditions in eight muscadine grape (vitis rotundifolia) cultivars and evaluated for antioxidant capacity as influenced by two ripening stages and their location within the fruit (skin, pulp, and juice). all polyphenolics generally increased as fruit ripened and the highest concentrations were located in the skins. free ellagic acid, ellagic acid gl ... | 2004 | 14733522 |
| inhibition of metalloproteinase activity by fruit extracts. | while the metalloproteinase enzymes are essential for development and remodeling of tissues, aberrant over expression of these enzymes contributes to several pathologic conditions. in particular, metalloproteinase over expression in cancer plays a significant role in metastasis by providing a mechanism for invasion and spread. the data presented here indicate that water extracts of raspberries, blackberries and muscadine grapes inhibit the activities of metalloproteinases 2 and 9. this inhibitio ... | 2004 | 15279895 |
| ellagic acid and quercetin interact synergistically with resveratrol in the induction of apoptosis and cause transient cell cycle arrest in human leukemia cells. | anticarcinogenic effects of polyphenolic compounds in fruits and vegetables are well established. although polyphenols naturally occur as combinations, little information is available regarding possible synergistic or antagonistic biochemical interactions between compounds. identifying potential interactions between polyphenols may provide information regarding the efficiency of polyphenol-containing foods in cancer prevention. the objective of this study was to investigate the interactions of e ... | 2005 | 15670891 |
| genetic and physical mapping of the grapevine powdery mildew resistance gene, run1, using a bacterial artificial chromosome library. | resistance to grapevine powdery mildew is controlled by run1, a single dominant gene present in the wild grapevine species, muscadinia rotundifolia, but absent from the cultivated species, vitis vinifera. run1 has been introgressed into v. vinifera using a pseudo-backcross strategy, and genetic markers have previously been identified that are linked to the resistance locus. here we describe the construction of comprehensive genetic and physical maps spanning the resistance locus that will enable ... | 2005 | 15902396 |
| identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by hplc-esi-ms. | ellagic acid, ellagic acid glycosides, and ellagitannins found in various fruits and nuts, including muscadine grape, are reported to have potential health-promoting benefits and antioxidant properties. this study isolated and identified several ellagic acid derivatives present in muscadine grapes and determined their relative antioxidant properties (aox). compounds were extracted from grape skins and pulp using methanol, and the solvent was evaporated. isolates were dissolved in citric acid buf ... | 2005 | 16028988 |
| antiinflammatory properties of the muscadine grape (vitis rotundifolia). | the muscadine grape possesses one of the highest antioxidant levels among fruits; yet, the effect of this fruit on mammalian metabolic systems has not received significant attention. to examine the antiinflammatory properties of the muscadine, grape skins were dried, pulverized, and extracted (10% w/v) with 50% ethanol. the extract was then tested in two different assays: the release of superoxide in phorbol myristate acetate-activated neutrophils and the release of cytokines [tumor necrosis fac ... | 2005 | 16248541 |
| study of anticancer activities of muscadine grape phenolics in vitro. | muscadine grapes have unique aroma and flavor characteristics. although a few studies reported high polyphenols content of muscadine grapes, little research has been conducted to evaluate the phenolic compounds bioactivities in any muscadine grape cultivar. the objective of this study was to evaluate the effect of phenolic compounds in muscadine grapes on cancer cell viability and apoptosis. four cultivars of muscadine (carlos, ison, noble, and supreme) were assessed in this study. phenolic comp ... | 2005 | 16248588 |
| induction of cell death in caco-2 human colon carcinoma cells by ellagic acid rich fractions from muscadine grapes (vitis rotundifolia). | possible anticancer mechanisms exerted by polyphenolic compounds contained in fruits and vegetables include antioxidant activity, the inhibition of proliferation, and the induction of apoptosis in cancer cells. this study examined the effects of four isolated polyphenolic extracts from red muscadine grapes (vitis rotundifolia) on vital cell parameters and the induction of apoptosis in caco-2 colon carcinoma cells. the magnitude of effects in cell culture was then correlated to polyphenolic compo ... | 2006 | 16848514 |
| microbial stability, phytochemical retention, and organoleptic attributes of dense phase co2 processed muscadine grape juice. | dense phase co2 processing (dp-co2) is a promising alternative to thermal pasteurization potentially inactivating microorganisms without affecting food phytochemicals or organoleptic characteristics. to demonstrate these effects, studies were conducted by changing processing pressure and co2 concentration in relation to microbial destruction. subsequent storage stability (10 weeks at 4 degrees c) of muscadine grape juice processed by dp-co2 (34.5 mpa at 8% or 16% co2) was evaluated and compared ... | 2006 | 16848533 |
| enhancing the retention of phytochemicals and organoleptic attributes in muscadine grape juice through a combined approach between dense phase co2 processing and copigmentation. | this study evaluated the phytochemical stability and organoleptic attributes of an ascorbic acid-fortified muscadine grape juice as affected by dense phase co2 processing (dp-co2) and addition of thyme polyphenolic cofactors (thymus vulgaris; 1:100 anthocyanin-to-cofactor molar ratio) in efforts to prevent phytochemical losses that occur during storage of anthocyanin-containing beverages, especially in the presence of carbonyl compounds commonly produced during thermal processing and storage. dp ... | 2006 | 16939329 |
| muscadine grape products intake, diet and blood constituents of non-diabetic and type 2 diabetic subjects. | red wines and grape juices contain polyphenolics with antioxidant and antiplatelet properties that may be protective against oxidative stress leading to hypertension, insulin resistance, and type 2 diabetes (t2d). this study evaluated the effects of supplementing meals of subjects with 150 ml of muscadine grape juice (mj), muscadine grape wine (mw), and dealcoholized muscadine grape wine (dz-w) on glycemic indices, blood constituents, lipid profile, anthropometric, and nutrient intakes of health ... | 2017 | 17030113 |
| anticancer effects of four varieties of muscadine grape. | the initiating event in carcinogenesis is a somatic mutation. during progression of the disease, additional mutations accumulate as the transformed cells develop the ability to proliferate and metastasize. these mutations can be produced by reactive oxygen species (ros) generated through metabolism, or environmental insult. metastasis involves tissue degradative enzymes, many of which are members of the matrix metalloproteinase family. hence, substances that can neutralize ros, inhibit mutagenes ... | 2007 | 17472467 |
| influence of xylem fluid chemistry on planktonic growth, biofilm formation and aggregation of xylella fastidiosa. | xylella fastidiosa is the causal agent of pierce's disease in grapevines. the mechanisms of pathogenicity are largely due to occlusion of xylem vessels by aggregation of x. fastidiosa and biofilm formation. xylella fastidiosa was subjected to xylem fluids with varying chemistries to examine the effects of nutritional components on bacterial growth in vitro. the exposure of x. fastidiosa to xylem fluids collected from different vitis genotypes resulted in highly significant differences in both pl ... | 2007 | 17610515 |
| inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms. | the phytochemical resveratrol contained in red grapes has been shown to inhibit prostate cancer cell growth, in part, through its antioxidant activity. muscadine grapes contain unique phytochemical constituents compared with other grapes and are potentially a source for novel compounds with antitumor activities. we compared the antitumor activities of muscadine grape skin extract (mske), which we show contains no resveratrol, with that of resveratrol using primary cultures of normal prostate epi ... | 2007 | 17804756 |
| untranslated leader region polymorphism of tvv1, a retrotransposon family, is a novel marker useful for analyzing genetic diversity and relatedness in the genus vitis. | grapevine retrotransposons belonging to the tvv1 family share a single, highly conserved open reading frame but differ by their untranslated leader (utl) region, which is highly variable in size. amplification of the utl region of tvv1 elements from 94 vitaceae accessions reveals that each of them shows a unique pattern of utl-derived bands, which is inherited in progenies but conserved between clones vegetatively propagated. the overall organization of genetic diversity of the vitaceae at the i ... | 2007 | 17926019 |
| stability of copigmented anthocyanins and ascorbic acid in muscadine grape juice processed by high hydrostatic pressure. | intermolecular copigmentation is one of the mechanisms of stabilization of anthocyanins in nature and is also responsible for the characteristic color and stability of aged red wines. in the present study, the effect of polyphenol oxidase (ppo) activity on phytochemical stability of an ascorbic acid-fortified muscadine grape juice following high hydrostatic pressure (hhp) processing (400 and 550 mpa for 15 min) and after 21 d of storage at 25 degrees c was investigated. addition of rosemary and ... | 2007 | 17995786 |
| topical anti-inflammatory activities of vitis rotundifolia (muscadine grape) extracts in the tetradecanoylphorbol acetate model of ear inflammation. | the ability of muscadine grape skin, seed, or combined skin and seed extracts to inhibit mouse ear inflammation, edema, and polymorphonuclear leukocyte infiltration was tested following topical application of 12-o-tetradecanoylphorbol 13-acetate (tpa). ethanolic extracts of skins, seeds, or a combination of these from purple (ison) cultivars were applied to both ears of female swiss mice 30 minutes after tpa (2 microg per ear) administration. control mice were treated with indomethacin or 50% et ... | 2007 | 18158834 |
| agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in vitis rotundifolia michx. (muscadine grape). | a method to produce transgenic plants of vitis rotundifolia was developed. embryogenic cultures were initiated from leaves of in vitro grown shoot cultures and used as target tissues for agrobacterium-mediated genetic transformation. a green fluorescent protein/neomycin phosphotransferase ii (gfp/nptii) fusion gene that allowed for simultaneous selection of transgenic cells based on gfp fluorescence and kanamycin resistance was used to optimize parameters influencing genetic transformation. it w ... | 2008 | 18256837 |
| influence of pressing method on juice stilbene content in muscadine and bunch grapes. | juice stilbene contents of 2 muscadine (vitis rotundifolia) cultivars, noble and carlos, and 2 bunch grape (vitis labrusca) cultivars, mid south and miss blanc were evaluated after crushing and pressing. four commercial pressing methods were used to produce juice: (1) cold press of fresh berries (cp); (2) hot press of fresh berries (hp); (3) cold press of frozen and thawed berries (fcp); and (4) cold press of pectolytic enzyme treated berries (ecp). free run juice (frj) from the crusher without ... | 2008 | 18460131 |
| inhibition of protein glycation by skins and seeds of the muscadine grape. | the formation of advanced glycation end products (ages) leading to protein glycation and cross-linking is associated with the pathogenesis of diabetic complications. the inhibition of protein glycation by phenolic and flavonoid antioxidants demonstrates that the process is mediated, in part, by oxidative processes. in this study, the effects of seed and skin extracts of the muscadine grape on ages formation were examined. seeds and skins were extracted (10% w/v) with 50% ethanol and incubated at ... | 2007 | 18525113 |
| antimicrobial effect of water-soluble muscadine seed extracts on escherichia coli o157:h7. | water-soluble extracts were prepared from purple (cultivar ison) and bronze (cultivar carlos) muscadine seeds with or without heating. the ison extracts had strong antimicrobial activity against a cocktail of three strains of escherichia coli o157: h7. this extract had higher acidity (ph 3.39 to 3.43), total phenolics (2.21 to 3.49 mg/ml), tartaric acid (5.6 to 10.7 mg/ml), tannic acid (5.7 to 8.1 mg/ml), and gallic acid (0.33 to 0.59 mg/ml) than did the carlos extracts. heat treatment on both e ... | 2008 | 18680948 |
| ultrastructure of tuta absoluta parasitized eggs and the reproductive potential of females after parasitism by metarhizium anisopliae. | among the microorganisms used in biological control, the muscadine fungus metarhizium anisopliae (metsch.) sorok. is produced and formulated world wide aiming to control pests from several agricultural crops. this work evaluated effects of m. anisopliae isolate ufrpe-6 on the fecundity and mortality of tuta absoluta (lepidoptera: gelechiidae) females and the mechanism of infection on eggs. the infection of the females by the fungus did not affect their oviposition and fecundity; however it affec ... | 2009 | 18789707 |
| nonhost versus host resistance to the grapevine downy mildew, plasmopara viticola, studied at the tissue level. | following inoculation of host and nonhost plants with plasmopara viticola, the grapevine downy mildew, a histological survey was undertaken to identify the stage where its development is contained in nonhosts and in resistant host plants. three herbaceous nonhost species, beta vulgaris, lactuca sativa, and capsicum annuum, and three grapevine species displaying different level of resistance (vitis vinifera [susceptible], vitis riparia [partially resistant] and muscadinia rotundifolia [totally re ... | 2008 | 18943253 |
| antibacterial effects of grape extracts on helicobacter pylori. | anti-helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phenolic levels do not necessarily determine overall anti-h. pylori efficacy. | 2009 | 19047390 |
| a snp transferability survey within the genus vitis. | efforts to sequence the genomes of different organisms continue to increase. the dna sequence is usually decoded for one individual and its application is for the whole species. the recent sequencing of the highly heterozygous vitis vinifera l. cultivar pinot noir (clone entav 115) genome gave rise to several thousand polymorphisms and offers a good model to study the transferability of its degree of polymorphism to other individuals of the same species and within the genus. | 2008 | 19087337 |
| heterogeneity in red wine polyphenolic contents differentially influences alzheimer's disease-type neuropathology and cognitive deterioration. | we recently found that moderate consumption of two unrelated red wines generate from different grape species, a cabernet sauvignon and a muscadine wine that are characterized by distinct component composition of polyphenolic compounds, significantly attenuated the development of alzheimer's disease (ad)-type brain pathology and memory deterioration in a transgenic ad mouse model. interestingly, our evidence suggests that the two red wines attenuated ad phenotypes through independent mechanisms. ... | 2009 | 19158422 |
| enhancement of resistance to coccidiosis and necrotic enteritis in broiler chickens by dietary muscadine pomace. | muscadine pomace (mp), a by-product of the production of wine and juice from vitis rotundifolia, was dried and tested in chickens for effects on primary resistance to coccidiosis, development of protective immunity after vaccination with live coccidia, and resistance to necrotic enteritis (ne) caused by the joint action of clostridium perfringens and coccidia. to test primary resistance to coccidiosis, 2-wk-old chicks were given 2% or 5% mp in the diet and inoculated with eimeria acervulina and ... | 2008 | 19166057 |
| inactivation of enterobacter sakazakii by water-soluble muscadine seed extracts. | hot and cold water-soluble muscadine (vitis rotundifolia) seed extracts and their polar and polyphenol fractions from two muscadine cultivars ('ison', purple and 'carlos', bronze) were investigated for their inhibition of enterobacter sakazakii. the heat treatment on each seed extract not only increased total phenolics and tannic acid but also enhanced antimicrobial activity against two strains of e. sakazakii. within 1 h, all seed extracts reduced an initial population (approximately 6 log cfu/ ... | 2009 | 19167124 |
| antibacterial activity of fresh and processed red muscadine juice and the role of their polar compounds on escherichia coli o157:h7. | the objectives of this research were to show the anti-escherichia coli o157:h7 effect of fresh (frmj) and processed red muscadine (vitis rotundifolia) juice (prmj) and to discern the active compounds responsible for anti-e. coli o157:h7. | 2009 | 19302492 |
| proteomics approach to identify unique xylem sap proteins in pierce's disease-tolerant vitis species. | pierce's disease (pd) is a destructive bacterial disease of grapes caused by xylella fastidiosa which is xylem-confined. the tolerance level to this disease varies among vitis species. our research was aimed at identifying unique xylem sap proteins present in pd-tolerant vitis species. the results showed wide variation in the xylem sap protein composition, where a set of polypeptides with pi between 4.5 and 4.7 and m(r) of 31 kda were present in abundant amount in muscadine (vitis rotundifolia, ... | 2010 | 19412582 |
| color and polyphenolic stability in extracts produced from muscadine grape (vitis rotundifolia) pomace. | the muscadine grape ( vitis rotundifolia ) industry of the southern united states is largely devoid of value-added processes that capture the phytochemical content of wine and juice byproducts. methods to recover and stabilize polyphenolics from muscadine grape pomace following juice manufacture were evaluated in laboratory-scale and pilot-scale trials. in laboratory-scale trials using osmotic equilibration, water-based extracts from juice pomace initially extracted 31-42% of total polyphenolics ... | 2009 | 19754172 |
| antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of vitis rotundifolia (muscadine grapes) as determined by hplc-dad-esi-ms(n). | the objective of this study was to determine and compare the total phenolic content and antioxidant capacity in the seeds, skin, and pulp of eight cultivars of florida-grown muscadine grapes and to identify the phenolic compounds in these respective portions. total phenolic contents were determined colorimetrically using folin-ciocalteu reagent, and antioxidant capacity was determined by oxygen radical absorbance capacity (orac). high-performance liquid chromatography equipped with diode array ( ... | 2010 | 20334341 |
| identification of natural antimicrobial substances in red muscadine juice against cronobacter sakazakii. | red muscadine (vitis rotundifolia michx.) juices with natural organic, phenolic acids and polyphenol compounds were tested against cronobacter sakazakii. the concentration of total phenolic compounds of commercial baby juices ranged from 176.7 to 347.7 mg/ml. commercial baby juices showed poor antimicrobial activity, reducing less than 1-log of c. sakazakii in juice samples for 2 h at 37 degrees c. red muscadine juices, regardless of processing methods (filtration, pasteurization, and sterilizat ... | 2010 | 20492304 |
| inhibition of oxidative hemolysis by quercetin, but not other antioxidants. | we previously reported that lipid-soluble quercetin, not water-soluble dihydroquercetin, protects human red blood cells against oxidative damage. the objectives of this study were to determine if an antihemolytic effect could be produced by other lipid-soluble antioxidants and if anti-inflammatory activity played a role in antihemolysis by quercetin. this study compared three lipid-soluble polyphenols, muscadine, curcumin and quercetin, and three lipid- (alpha-tocopherol and alpha-tocotrienol) o ... | 2010 | 20510893 |
| phylogeography and population structure of the grape powdery mildew fungus, erysiphe necator, from diverse vitis species. | the grape powdery mildew fungus, erysiphe necator, was introduced into europe more than 160 years ago and is now distributed everywhere that grapes are grown. to understand the invasion history of this pathogen we investigated the evolutionary relationships between introduced populations of europe, australia and the western united states (us) and populations in the eastern us, where e. necator is thought to be native. additionally, we tested the hypothesis that populations of e. necator in the e ... | 2010 | 20809968 |
| variation in pathogenicity and aggressiveness of erysiphe necator from different vitis spp. and geographic origins in the eastern united states. | eastern north america is considered the center of diversity for many vitis spp. and for the grape powdery mildew pathogen, erysiphe necator. however, little is known about populations of e. necator from wild vitis spp. we determined the phenotypic variation in pathogenicity and aggressiveness of e. necator among isolates from wild and domesticated vitis spp. from diverse geographic regions in the eastern united states. to test pathogenicity, we inoculated 38 e. necator isolates on three wild vit ... | 2010 | 20932167 |
| activities of muscadine grape skin and quercetin against helicobacter pylori infection in mice. | to explore the preventative potential of muscadine grape skin (mgs) and the single flavonoid, quercetin, as an alternative means for ameliorating helicobacter pylori infection and/or the h. pylori-induced inflammatory response in mice. methods and results: the antimicrobial and anti-inflammatory properties of mgs and quercetin, a major phenolic constituent, were evaluated against h. pylori in vitro and in vivo. the antimicrobial activity of quercetin was evaluated against 11 h. pylori strains in ... | 2010 | 20955190 |
| using a limited mapping strategy to identify major qtls for resistance to grapevine powdery mildew (erysiphe necator) and their use in marker-assisted breeding. | a limited genetic mapping strategy based on simple sequence repeat (ssr) marker data was used with five grape populations segregating for powdery mildew (erysiphe necator) resistance in an effort to develop genetic markers from multiple sources and enable the pyramiding of resistance loci. three populations derived their resistance from muscadinia rotundifolia 'magnolia'. the first population (06708) had 97 progeny and was screened with 137 ssr markers from seven chromosomes (4, 7, 9, 12, 13, 15 ... | 2010 | 21188350 |
| the effectiveness of stilbenes in resistant vitaceae: ultrastructural and biochemical events during plasmopara viticola infection process. | leaves of different vitis vinifera l. cultivars, susceptible or resistant to downy mildew, chasselas, solaris, irac 2091 (cvs. gamaret x bronner) and muscadinia rotundifolia were inoculated with plasmopara viticola. samples were then examined by scanning and transmission electron microscopy, by light microscopy and for their ability to synthesise stilbenes. these phytoalexins were strictly analysed at infection sites. in the susceptible chasselas, p. viticola colonises, at 72h post-infection (hp ... | 2010 | 21256040 |
| a resveratrol and polyphenol preparation suppresses oxidative and inflammatory stress response to a high-fat, high-carbohydrate meal. | high-fat, high-carbohydrate (hfhc) meals are known to induce oxidative and inflammatory stress, an increase in plasma endotoxin concentrations, and an increase in the expression of suppressor of cytokine signaling-3 (socs-3). | 2011 | 21289251 |
| an expedient and versatile protocol for extracting high-quality dna from plant leaves. | introductionprotocols for extracting plant genomic dna have to contend with two major challenges: preventing the oxidation of phenolic substances that can react with nucleic acids and proteins, and eliminating polysaccharides that interfere with downstream enzymatic manipulations of dna. a protocol has been developed in which the above-mentioned substances are removed from the sample before the dna is removed from the chromatin structure. this protocol requires only two tubes during extraction a ... | 2007 | 21357104 |
| effect of muscadine grape seed supplementation on vascular function in subjects with or at risk for cardiovascular disease: a randomized crossover trial. | muscadine grape seeds have high concentrations of polyphenolic compounds with antioxidant and other properties that would be expected to have favorable effects on endothelial function. | 2010 | 21504973 |
| resveratrol inhibits periodontal pathogens in vitro. | the gram-negative anaerobic bacteria a. actinomycetemcomitans (aa) and p. gingivalis (pg) are key components in the aetiology of periodontal disease, and associated hard-tissue destruction. resveratrol is a phytoalexin, produced naturally by several plants when under attack by bacterial or fungal pathogens. it is found in many foods including mulberries, peanuts and the skin of labrusca and muscadine grapes. the objective of this study was to evaluate the effect of resveratrol on the in vitro gr ... | 2011 | 21520308 |
| antibacterial activities of blueberry and muscadine phenolic extracts. | phenolics are one category of phyto-antimicrobials that refer to the antimicrobial substances extracted from plant sources. this study was undertaken to determine the influence of blueberry and muscadine phenolic extracts on the growths of 2 important foodborne bacterial pathogens, salmonella enteritidis and listeria monocytogenes. cells of s. enteritidis (n = 4) or l. monocytogenes (n = 4) strains were inoculated (3 log cfu/ml) into tryptic soy broth (tsb) supplemented with 46.25 ppm of muscadi ... | 2011 | 21535771 |
| the structure of xylem vessels in grapevine (vitaceae) and a possible passive mechanism for the systemic spread of bacterial disease. | xylem-dwelling pathogens become systemic, suggesting that microorganisms move efficiently in the xylem. to better understand xylem pathways and how bacteria move within the xylem, vessel connectivity between stems and leaves of vitis vinifera cv. chardonnay and muscadinia rotundifolia cv. cowart was studied. three methods were used: (1) the light-producing bacterium, yersinia enterocolitica, (ye) strain gy5232 was loaded into petioles and followed using x-ray film, (2) fluorescent beads were loa ... | 2006 | 21646209 |
| Polyphenolics from ac¦ºai¦ü ( Euterpe oleracea Mart.) and red muscadine grape (Vitis rotundifolia ) protect human umbilical vascular Endothelial cells (HUVEC) from glucose- and lipopolysaccharide (LPS)-induced inflammation and target microRNA-126. | Endothelial anti-inflammatory effects of ac¦ºai¦ü (Ac) and red muscadine grape (Gp) polyphenolics have not been extensively investigated. It was hypothesized that polyphenolics from Ac and Gp exert comparable protective effects in human vascular endothelial cells (HUVEC) upon inflammatory stress. Furthermore, this study investigated whether microRNAs relevant to endothelial function might be regulated by Ac and Gp. Results showed that Ac and Gp (5-20 mg gallic acid equivalent/L) protected HUVEC ... | 2011 | 21682256 |
| resistance to elsinoë ampelina and expression of related resistant genes in vitis rotundifolia michx. grapes. | muscadine grapes (vitis rotundifolia michx) are considered as excellent genetic resources for grape breeding programs as they are known for their hardiness and resistance to pests and diseases. however, contrary to popular belief, our study indicated that not all muscadine cultivars are resistant to anthracnose disease. in order to identify a source of genetic tolerance towards anthracnose among muscadine cultivars, a series of in-situ and ex-situ experiments were conducted through strict and se ... | 2011 | 21747689 |
| inhibitory effects of muscadine anthocyanins on α-glucosidase and pancreatic lipase activities. | inhibitory effects of the noble muscadine grape extracts and the representative phytochemicals for anthocyanins (i.e., cyanidin and cyanidin-3,5-diglucoside) on two enzymes, that is, α-glucosidase and pancreatic lipase, were investigated regarding their antidiabetic activities. the study demonstrated that the anthocyanin extracts and the selected chemicals obeyed the competitive mode against the enzymes. the methanolic extracts of whole fruit and skin of the muscadine showed inhibitory activitie ... | 2011 | 21797278 |
| mining and validating grape (vitis l.) ests to develop est-ssr markers for genotyping and mapping. | grape expressed sequence tags (ests) are a new resource for developing simple sequence repeat (ssr) functional markers for genotyping and genetic mapping. an integrated pipeline including several computational tools for ssr identification and functional annotation was developed to identify 6,447 est-ssr sequences from a total collection of 215,609 grape ests retrieved from ncbi. the 6,447 est-ssrs were further reduced to 1,701 non-redundant sequences via clustering analysis, and 1,037 of them we ... | 2011 | 21841909 |
| comparison of odor-active compounds in grapes and wines from vitis vinifera and non-foxy american grape species. | native american grape (vitis) species have many desirable properties for winegrape breeding, but hybrids of these non-vinifera wild grapes with vitis vinifera often have undesirable aromas. other than the foxy-smelling compounds in vitis labrusca and vitis rotundifolia , the aromas inherent to american vitis species are not well characterized. in this paper, the key odorants in wine produced from the american grape species vitis riparia and vitis cinerea were characterized in comparison to wine ... | 2011 | 21879766 |
| Identification of Race-Specific Resistance in North American Vitis spp. Limiting Erysiphe necator Hyphal Growth. | ABSTRACT Race-specific resistance against powdery mildews is well documented in small grains but, in other crops such as grapevine, controlled analysis of host-pathogen interactions on resistant plants is uncommon. In the current study, we attempted to confirm powdery mildew resistance phenotypes through vineyard, greenhouse, and in vitro inoculations for test cross-mapping populations for two resistance sources: (i) a complex hybrid breeding line, 'Bloodworth 81-107-11', of at least Vitis rot ... | 2012 | 22165984 |
| a review on chemical and biological properties of cayratia trifolia linn. (vitaceae). | cayratia trifolia linn. domin syn. vitis trifolia (family: vitaceae) is commonly known as fox grape in english; amlabel, ramchana in hindi and amlavetash in sanskrit. it is native to india, asia and australia. it is a perennial climber having trifoliated leaves with 2-3 cm long petioles and ovate to oblong-ovate leaflets. flowers are small greenish white and brown in color. fruits are fleshy, juicy, dark purple or black, nearly spherical, about 1 cm in diameter. it is found throughout the hills ... | 2011 | 22279376 |
| elevated gene expression in chalcone synthase enzyme suggests an increased production of flavonoids in skin and synchronized red cell cultures of north american native grape berries. | anthocyanins are antioxidants and are among the natural products synthesized via the flavonoid biosynthesis pathway. anthocyanins have been recommended for dietary intake in the prevention of cardiovascular diseases, cancer, and age-related conditions such as alzheimer's disease or dementia. with an increasingly aging population in many parts of the world, strategies for the commercial production of in vitro synchronized red cell cultures as natural antioxidants will be a significant contributio ... | 2012 | 22364203 |
| phenolic contents and compositions in skins of red wine grape cultivars among various genetic backgrounds and originations. | in order to analyze and compare the phenolic characteristics of red wine grapes with diverse genetic backgrounds, skin phenolics among 21 different cultivars belonging to vitis vinifera l., east asian and north american vitis species and hybrids, as well as 2 varieties of muscadine grapes were estimated by hplc-ms/ms. there were 45 anthocyanins, 28 flavonols, 8 flavan-3-ols, 9 cinnamic acids, 5 benzoic acids, 5 ellagic acids and 2 stilbenes detected in all the samples. total contents of each phe ... | 2012 | 22489164 |
| high-performance liquid chromatography-mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes. | evaporative light scattering detector (elsd) is considered as a universal detector able to detect almost any compound. this study successfully established a hplc-elsd method for the simultaneous determination of phenolic acids, flavonoids and anthocyanins in muscadine grape. twenty-four phenolics, including 5 anthocyanins, were identified by hplc-uv-ms, ten of them were selected to calibrate the elsd. results from the elsd quantification suggested that gallic acid, proanthocyanidins, and ellagic ... | 2012 | 22520637 |
| a framework genetic map of muscadinia rotundifolia. | this study presents a framework linkage map based on microsatellite markers for muscadinia rotundifolia (1n = 20). the mapping population consisted of 206 progeny generated from a cross of two m. rotundifolia varieties, 'fry' and 'trayshed'. a total of 884 primers were tested for their ability to amplify markers: 686 amplified and 312 simple sequence repeat (ssr) primer pairs generated 322 polymorphic markers for either one or both parents. the map for the female parent 'fry' consisted of 212 ma ... | 2012 | 22688272 |
| muscadine grape (vitis rotundifolia) and wine phytochemicals prevented obesity-associated metabolic complications in c57bl/6j mice. | the objective of this study was to determine the effects of muscadine grape or wine (cv. noble) phytochemicals on obesity and associated metabolic complications. muscadine grape or wine phytochemicals were extracted using amberlite fpx66 resin. male c57bl/6j mice were given a low-fat diet (lf, 10% kcal fat), high-fat diet (hf, 60% kcal fat), hf + 0.4% muscadine grape phytochemicals (hf+mgp), or hf + 0.4% muscadine wine phytochemicals (hf+mwp) for 15 weeks. at 7 weeks, mice fed hf+mgp had signifi ... | 2012 | 22788667 |
| a reference genetic map of muscadinia rotundifolia and identification of ren5, a new major locus for resistance to grapevine powdery mildew. | muscadinia rotundifolia, a species closely related to cultivated grapevine vitis vinifera, is a major source of resistance to grapevine downy and powdery mildew, two major threats to cultivated traditional cultivars of v. vinifera respectively caused by the oomycete plasmopara viticola and the ascomycete erisyphe necator. the aim of the present work was to develop a reference genetic linkage map based on simple sequence repeat (ssr) markers for m. rotundifolia. this map was created using s1 m. r ... | 2012 | 22865124 |
| analysis of phenolic composition of noble muscadine (vitis rotundifolia) by hplc-ms and the relationship to its antioxidant capacity. | phenolic compounds and anthocyanins in muscadines have attracted much attention due to their diverse biological activities. with bioassays of antioxidant activities in terms of total phenolic content (tpc), total anthocyanin content (tac), total procyanidin content (tpa), oxygen radical absorbance capacity (orac), and ferric reducing antioxidant power (frap) of different parts of the noble muscadine, the butanol (buoh) extract of the muscadine skin showed the highest tpc (317.91 ± 1.83 mg gae/10 ... | 2012 | 22924759 |
| stilbenoid profiles of canes from vitis and muscadinia species. | we present stilbenoid profiles of canes from 16 grapevines. fifteen stilbenoids were obtained through isolation and structure identification using ms, nmr, and [α](d) or as commercial standards. an hplc-uv method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of vitis amurensis, vitis arizonica, vitis berlandieri, vitis betulifolia, vitis cinerea, vitis × champini, vitis × doaniana, vitis labrusca, vitis candicans (syn. vitis mustangensis), vi ... | 2013 | 23270496 |
| identification of muscadine wine sulfur volatiles: pectinase versus skin-contact maceration. | muscadine grapes ( vitis rotundifolia ) are widely grown in the southern united states, as the more common vitis vinifera cannot be cultivated due to pierce's disease. there is interest to determine if certain cultivars can be used for good-quality wine production. this study compared the effect of pectolytic enzyme pretreatment with conventional skin-contact fermentation on muscadine (noble, vitis rotundifolia ) wine major volatiles, aroma active volatiles, and volatile sulfur compounds (vscs). ... | 2013 | 23289372 |
| activities of muscadine grape skin and polyphenolic constituents against helicobacter pylori. | to identify active phenolic constituents in muscadine grape skin (mgs) extracts and determine interactions among compounds while further exploring their anti-helicobacter pylori potential in vitro. | 2013 | 23294280 |
| adsorption/desorption characteristics and separation of anthocyanins from muscadine (vitis rotundifolia) juice pomace by use of macroporous adsorbent resins. | in this study, the adsorption/desorption characteristics of anthocyanins on five amberlite resins (fpx-66, xad-7hp, xad-16n, xad-1180, and xad-761) were evaluated. fpx-66 and xad-16n showed the highest adsorption and desorption capacities and ratios for anthocyanins from muscadine pomace extract, while xad-7hp had the lowest adsorption and desorption capacities and ratios. on the basis of static adsorption and desorption tests, three resins (fpx-66, xad-16n, and xad-1180) were selected for adsor ... | 2013 | 23368425 |
| resistance of grape rootstocks to plant-parasitic nematodes. | candidate grape rootstocks were selected through a rigorous screening program initiated with important sources of resistance to meloidogyne pathotypes and to xiphinema index in muscadinia rotundifolia and vitis species native to north america. based on their rooting capability and horticultural characteristics, 200 candidates were selected from 5,000 progeny of multiple crosses between commercial grape rootstocks and wild grape species that exhibited resistance to nematodes. after a 15-year scre ... | 2012 | 23482972 |
| bioactive polyphenols from muscadine grape and blackcurrant stably concentrated onto protein-rich matrices for topical applications. | natural botanical agents that are antimicrobial, or that modulate skin hyperpigmentation via tyrosinase inhibition, are increasingly sought in the cosmetic industry. | 2013 | 23614501 |
| antioxidant activity of selected stilbenoids and their bioproduction in hairy root cultures of muscadine grape (vitis rotundifolia michx.). | stilbenoids are polyphenolic phytoalexins with health-related properties in humans. muscadine grape ( vitis rotundifolia ) hairy root cultures were established via agrobacterium rhizogenes -mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and methyl jasmonate (meja) on stilbenoid production were studied. twenty-one-day-old hairy root cultures were treated with 100 μm meja for 24 h, and then the stilbenoids were extracted from the medium ... | 2013 | 23668830 |
| biological significance of urolithins, the gut microbial ellagic acid-derived metabolites: the evidence so far. | the health benefits attributed to pomegranate have been associated with its high content in polyphenols, particularly ellagitannins. this is also the case for other ellagitannin-containing fruits and nuts including strawberry, raspberry, blackberry, walnuts, and muscadine grapes. the bioavailability of ellagitannins and ellagic acid is however very low. these molecules suffer extensive metabolism by the gut microbiota to produce urolithins that are much better absorbed. urolithins circulate in p ... | 2013 | 23781257 |
| incorrectly annotated keratin derived peptide sequences lead to misleading ms/ms data interpretation. | in the course of the nanolc-nanoesi-ms/ms analysis of chelidonium majus proteins we detected an extremely abundant 12 aa peptide (sequence: tnaenefvtikk). the mascot search against ncbinr database with viridiplantae taxonomic restriction revealed its complete identity to unknown protein 18 from pseudotsuga menziesii (p85925). the same sequence has also been submitted as unknown protein 1 (p86104) from vitis rotundifolia and as a part of the oxygen-evolving enhancer protein 1 (oee1) from pinus st ... | 2013 | 23895828 |
| genetic dissection of a tir-nb-lrr locus from the wild north american grapevine species muscadinia rotundifolia identifies paralogous genes conferring resistance to major fungal and oomycete pathogens in cultivated grapevine. | the most economically important diseases of grapevine cultivation worldwide are caused by the fungal pathogen powdery mildew (erysiphe necator syn. uncinula necator) and the oomycete pathogen downy mildew (plasmopara viticola). currently, grapegrowers rely heavily on the use of agrochemicals to minimize the potentially devastating impact of these pathogens on grape yield and quality. the wild north american grapevine species muscadinia rotundifolia was recognized as early as 1889 to be resistant ... | 2013 | 24033846 |
| protoplast isolation and callus production from leaves of tissue-cultured vitis spp. | protoplasts were isolated from mesophyll of axenic cultures of grape, vitis rotundifolia cv. summit and v. vinifera cv. cabernet sauvignon. enzymes effective for protoplast isolation were macerozyme r-10 (0.5% and 0.1%) and cellulase onozuka r-10 (1.0% and 0.5%) for v. rotundifolia and v. vinifera, respectively. polyvinylpyrrolidone and 2-(n-morpholino)ethanesulfonic acid were essential in the isolation media. protoplasts were purified using flotation/centrifugation. the protoplasts of v. rotund ... | 1988 | 24240409 |
| itraq-based quantitative proteomics of developing and ripening muscadine grape berry. | grapes are among the widely cultivated fruit crops in the world. grape berries like other nonclimacteric fruits undergo a complex set of dynamic, physical, physiological, and biochemical changes during ripening. muscadine grapes are widely cultivated in the southern united states for fresh fruit and wine. to date, changes in the metabolites composition of muscadine grapes have been well documented; however, the molecular changes during berry development and ripening are not fully known. the aim ... | 2014 | 24251720 |
| differential induction of antioxidant stilbenoids in hairy roots of vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide. | stilbenoids are polyphenolic phytoalexins that exhibit potential health applications in humans. hairy root cultures of muscadine grape (vitis rotundifolia michx.) were used to study the biochemical and molecular regulation of stilbenoid biosynthesis upon treatment with 100 μm methyl jasmonate (meja) or 10 mm hydrogen peroxide (h2o2) over a 96-h period. resveratrol, piceid, and ε-viniferin were identified in higher concentrations in the tissue whereas resveratrol was the most abundant stilbenoid ... | 2014 | 24269870 |
| muscadine grape skin extract reverts snail-mediated epithelial mesenchymal transition via superoxide species in human prostate cancer cells. | snail transcription factor can induce epithelial-mesenchymal transition (emt), associated with decreased cell adhesion-associated molecules like e-cadherin, increased mesenchymal markers like vimentin, leading to increased motility, invasion and metastasis. muscadine grape skin extract (mske) has been shown to inhibit prostate cancer cell growth and induce apoptosis without affecting normal prostate epithelial cells. we investigated novel molecular mechanisms by which snail promotes emt in prost ... | 2014 | 24617993 |
| enzyme release of phenolics from muscadine grape (vitis rotundifolia michx.) skins and seeds. | enzyme degradation of plant cell wall polysaccharides can potentially enhance the release of bioactive phenolics. the aim of this study was to evaluate various combinations of solvent and enzyme, enzyme type (cellulase, pectinase, ß-glucosidase), and hydrolysis time (1, 4, 8, 24 h) on the release of muscadine grape skin and seed phenolics, and their antioxidant activities. results showed that pre-treated muscadine skins and seeds with enzymes decreased total phenolic yield compared with solvent ... | 2014 | 24679747 |
| ocular inflammation and endoplasmic reticulum stress are attenuated by supplementation with grape polyphenols in human retinal pigmented epithelium cells and in c57bl/6 mice. | inflammation and endoplasmic reticulum (er) stress are common denominators for vision-threatening diseases such as diabetic retinopathy and age-related macular degeneration. based on our previous study, supplementation with muscadine grape polyphenols (mgps) alleviated systemic insulin resistance and proinflammatory responses. in this study, we hypothesized that mgps would also be effective in attenuating ocular inflammation and er stress. we tested this hypothesis using the human retinal pigmen ... | 2014 | 24699803 |
| evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic dna markers associated with the resistant trait in chinese wild vitis. | the resistance of wild vitis germplasm, including chinese and american wild vitis and vitis vinifera cultivars, to powdery mildew (uncinula necator burr.) was evaluated for two consecutive years under natural conditions. most of the chinese and north american species displayed a resistant phenotype, whereas all of the european species were highly susceptible. the alachua and conquistador accessions of vitis rotundifolia species, which originated in north america, were immune to the disease, whil ... | 2014 | 24854440 |
| antioxidant, antibacterial, and antibiofilm properties of polyphenols from muscadine grape (vitis rotundifolia michx.) pomace against selected foodborne pathogens. | polyphenols are predominantly secondary metabolites in muscadine grapes, playing an important role in the species' strong resistance to pests and diseases. this study examined the above property by evaluating the antioxidant, antibacterial, and antibiofilm activities of muscadine polyphenols against selected foodborne pathogens. results showed that antioxidant activity for different polyphenols varied greatly, ranging from 5 to 11.1 mmol trolox/g. antioxidant and antibacterial activities for pol ... | 2014 | 24865879 |
| clinically relevant interaction between warfarin and scuppernongs, a quercetin containing muscadine grape: continued questions surrounding flavonoid-induced warfarin interactions. | we present a case of clinically relevant and probable interaction between warfarin and scuppernongs in a 73-year-old woman where ingestion of scuppernongs, a variety of quercetin-containing muscadine grapes, over a period of 2 months was associated with elevations in the international normalised ratio to supratherapeutic levels. while muscadine grapes and specifically scuppernongs are found primarily in southeastern usa, the flavonoid in questionand quercetin is found worldwide as a dietary supp ... | 2014 | 24966255 |
| fatty acid composition, oxidative stability, antioxidant and antiproliferative properties of selected cold-pressed grape seed oils and flours. | cold-pressed chardonnay, muscadine, ruby red, and concord grape seed oils and their defatted flours were studied for their fatty acid composition, oxidative stability and antioxidant and antiproliferative activities. the phenolic profiles of the seed flours were also measured. the most abundant fatty acid in the oils was linoleic acid, ranging from 66.0g/100g of total fatty acids in ruby red seed oil to 75.3g/100g of total fatty acids in concord seed oil. the oils were also high in oleic acid an ... | 2011 | 25212146 |
| concentrating immunoprotective phytoactive compounds from fruits and vegetables into shelf-stable protein-rich ingredients. | co-delivery of edible proteins with health-protective fruit (muscadine grape) and vegetable (kale) phytoactive compounds was accomplished in a biofortified ingredient for use in convenient, portable food formulations. polyphenolics were concentrated (10-42 mg/g range) in dry muscadine-protein matrices. kale-fortified protein matrices also captured polyphenolics (8 mg/g), carotenoids (69 μg/g) and glucosinolates (7 μmol/g). neither total phenolics nor glucosinolates were significantly diminished ... | 2014 | 25351938 |
| bioactive capacity, sensory properties, and nutritional analysis of a shelf stable protein-rich functional ingredient with concentrated fruit and vegetable phytoactives. | well-known health-protective phytochemicals from muscadine grape and kale were stably complexed with food grade protein (soy or hemp protein isolates) to create biofortified food ingredients for use in a variety of convenient, portable food formulations. the bioactive (anti-inflammatory) potential, sensory attributes and proximates of the prepared formulations were evaluated in this study. anti-inflammatory properties of the protein-phytoactive ingredient particles were contributed by the polyph ... | 2014 | 25359555 |
| ellagic acid modulates lipid accumulation in primary human adipocytes and human hepatoma huh7 cells via discrete mechanisms. | previously, we have reported that consumption of a muscadine grape phytochemical powder (mgp) decreased lipid accumulation in high-fat fed mice. the aim of this study was to identify the responsible polyphenolic constituents and elucidate the underlying mechanisms. in mice, mgp supplementation significantly reduced visceral fat mass as well as adipocyte size. to determine whether mgp affects adipogenesis or hypertrophic lipid accumulation, we used a human adipogenic stem cell (hascs) model. amon ... | 2015 | 25458530 |
| isolation of a wrky30 gene from muscadinia rotundifolia (michx) and validation of its function under biotic and abiotic stresses. | wrky transcription factors (tfs) play important roles in many plant processes, including responses to biotic and abiotic stresses. in the present study, muscadinia rotundifolia mrwrky30 dramatically accumulated in grapevine leaves in response to inoculation of plasmopara viticola, a pathogen causing grapevine downy mildew disease. similar responses were also found on grapevines treated with exogenous sa/ja/et. ectopic expression of mrwrky30 in arabidopsis thaliana "col0" enhanced its resistance ... | 2015 | 25643917 |
| ellagic acid induces cell cycle arrest and apoptosis through tgf-β/smad3 signaling pathway in human breast cancer mcf-7 cells. | breast cancer represents the second leading cause of cancer-related deaths among women worldwide and preventive therapy could reverse or delay the devastating impact of this disease. ellagic acid (ea), a dietary flavonoid polyphenol which is present in abundance in pomegranate, muscadine grapes, walnuts and strawberries, has been shown to inhibit cancer cells proliferation and induce apoptosis. here, we investigated the growth inhibitory effects of ea on mcf-7 breast cancer cells. in the present ... | 2015 | 25647396 |
| application of muscadine grape (vitis rotundifolia michx.) pomace extract to reduce carcinogenic acrylamide. | acrylamide is a byproduct of the maillard reaction and is formed in a variety of heat-treated commercial starchy foods. it is known to be toxic and potentially carcinogenic to humans. muscadine grape polyphenols and standard phenolic compounds were examined on the reduction of acrylamide in an equimolar asparagine/glucose chemical model, a potato chip model, and a simulated physiological system. polyphenols were found to significantly reduce acrylamide in the chemical model, with reduced rates h ... | 2015 | 25842328 |
| a phase i study of muscadine grape skin extract in men with biochemically recurrent prostate cancer: safety, tolerability, and dose determination. | new therapies are being explored as therapeutic options for men with biochemically recurrent prostate cancer (brpc) who wish to defer androgen deprivation therapy. mpx is pulverized muscadine grape (vitis rotundifolia) skin that contains ellagic acid, quercetin, and resveratrol and demonstrates preclinical activity against prostate cancer cells in vitro. | 2015 | 26012728 |
| strategies for run1 deployment using run2 and ren2 to manage grapevine powdery mildew informed by studies of race specificity. | the toll/interleukin-1 receptor nucleotide-binding site leucine-rich repeat gene, "resistance to uncinula necator 1" (run1), from vitis rotundifolia was recently identified and confirmed to confer resistance to the grapevine powdery mildew fungus erysiphe necator (syn. u. necator) in transgenic v. vinifera cultivars. however, sporulating powdery mildew colonies and cleistothecia of the heterothallic pathogen have been found on introgression lines containing the run1 locus growing in new york (ny ... | 2015 | 26039639 |
| extracts from red muscadine and cabernet sauvignon wines induce cell death in molt-4 human leukemia cells. | red wine contains a diversity of polyphenolic compounds that exert beneficial health effects including anti-cancer effects. this trial evaluated the anti-proliferative potential of red muscadine (vitis rotundifolia) and red cabernet sauvignon (vitis vinifera) wines in cell culture. chemical properties of wines were determined by hplc-pda analysis and concentrated extracts of each wine were evaluated before and after glycosidic hydrolysis in molt-4 leukemia cells. cell growth and the induction of ... | 2008 | 26065741 |
| muscadine grape skin extract can antagonize snail-cathepsin l-mediated invasion, migration and osteoclastogenesis in prostate and breast cancer cells. | to develop new and effective chemopreventive agents against bone metastasis, we assessed the effects of muscadine grape skin extract (mske), whose main bioactive component is anthocyanin, on bone turnover, using prostate and breast cancer cell models overexpressing snail transcription factor. mske has been shown previously to promote apoptosis in prostate cancer cells without affecting normal prostate epithelial cells. snail is overexpressed in prostate and breast cancer, and is associated with ... | 2015 | 26069256 |
| muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation. | tocotrienols are unsaturated forms of vitamin e previously shown to reduce adipogenesis and adipose inflammation. in this study, muscadine grape seed oil (mgso) was identified as a novel source of tocotrienols containing significant amounts of α- and γ-tocotrienol (t3) with minor seasonal changes. the aim of this study was to assess the anti-adipogenic and anti-inflammatory potential of mgso by using primary human adipose-derived stem cells (hascs). differentiating hascs were treated with mgso a ... | 2015 | 26073057 |