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primary analysis of dna polymorphisms in the trim region (mhc subregion) of the japanese quail, coturnix japonica.based on sequences of two cosmid clones from japanese quail (coturnix japonica, coja), we confirmed that the syntenic cluster, gnb2l1∼btn1∼btn2, is located in the quail trim subregion of the quail major histocompatibility complex (mhc coja) region. these cosmids also included four cjbg loci and one cjlec locus; therefore, the quail trim subregion was thought to be adjacent to the bg/lec subregion. we then identified three polymorphic markers - cjhep21, cjtrim39.2 and cjbtn2 - in the trim subregi ...201323302088
[infradian rhythms of the esophageal epithelium mitotic activity and corticosterone and thyroxin levels in japanese quails coturnix japonica].an everyday investigation of the dynamics of the blood serum corticosterone and thyroxin levels, as well as the esophageal epithelium proliferation activity of male japanese quails has been conducted over the period of 16 days. a 4-day rhythm of the corticosterone serum level occurring synchronically in the bodies of different individuals was discovered. a 3-day biorhythm of the thyroxin level was shown. the mitotic index figures in the esophageal epithelium negatively correlated with the cortic ...201324592742
a simple vitrification method for cryobanking avian testicular tissue.cryopreservation of testicular tissue is a promising method of preserving male reproductive potential for avian species. this study was conducted to assess whether a vitrification method can be used to preserve avian testicular tissue, using the japanese quail (coturnix japonica) as a model. a simple vitrification method that included dimethyl sulphoxide, ethylene glycol, and sucrose as cryoprotective agents, and allowed the storage of tissue in a sealed macrotube was applied to the testicular t ...201223155032
production of reactive oxygen species, gene expression, and enzymatic activity in quail subjected to acute heat stress.the aim of the present study was to evaluate the effect of acute heat stress on the production of mitochondrial reactive oxygen species (ros), the gene expression of the avian uncoupling protein (avucp) and glutathione peroxidase (gpx 7), and the activity of the enzyme gpx in the liver of meat quail. two groups of 15 meat quail (coturnix coturnix japonica) that were 23 d of age were initially housed individually in metallic cages. a period of 7 d was provided for the 2 bird groups to adapt to th ...201323148249
japanese quail acute exposure to methamidophos: experimental design, lethal, sub-lethal effects and cholinesterase biochemical and histochemical expression.we exposed the japanese quail (coturnix coturnix japonica) to the organophosphate methamidophos using acute oral test. mortality and sub-lethal effects were recorded in accordance to internationally accepted protocols. in addition cholinesterases were biochemically estimated in tissues of the quail: brain, liver and plasma. furthermore, brain, liver and duodenum cryostat sections were processed for cholinesterase histochemistry using various substrates and inhibitors. mortalities occurred mainly ...201323146311
centromere positions in chicken and japanese quail chromosomes: de novo centromere formation versus pericentric inversions.chicken (gallus gallus domesticus, gga) and japanese quail (coturnix coturnix japonica, cco) karyotypes are very similar. they have identical chromosome number (2n = 78) and show a high degree of synteny. centromere positions on the majority of orthologous chromosomes are different in these two species. to explore the nature of this divergence, we used high-resolution comparative fluorescent in situ hybridization mapping on giant lampbrush chromosomes (lbcs) from growing oocytes. we applied 41 b ...201223143647
changes in plasma gonadotrophins, 17β-oestradiol, progesterone, prolactin, thyroxine and triiodothyronine concentrations in female japanese quail (coturnix coturnix japonica) of a heavy body weight line during photo-induced ovarian growth and regression.1. simultaneous changes of the width of the cloacal opening and plasma luteinising hormone (lh), follicle stimulating hormone (fsh), 17β-oestradiol, progesterone, prolactin, thyroxine (t(4)) and triiodothyronine (t(3)) during photo-induced ovarian growth and regression were measured in commercially bred japanese quail from a heavy body weight line. 2. somatically mature female japanese quail were transferred from short days (light:dark 8l:16d) at 10°c to long days (16l:8d) at 20°c, and sexually ...201223130587
condition-dependent strategies of eggshell pigmentation: an experimental study of japanese quail (coturnix coturnix japonica).a relationship has been suggested between eggshell colour and female body condition based on the opposing antioxidant properties of the two main eggshell pigments: the antioxidant biliverdin (blue-green) and the pro-oxidant protoporphyrin (brown). we hypothesized that experimentally food-restricted females with low antioxidant capacity would deposit more protoporphyrin and less biliverdin in their eggshells, resulting in eggshells of reduced brightness but increased colour intensity. two eggs we ...201323125343
long-lasting and sex-specific consequences of elevated egg yolk testosterone for social behavior in japanese quail.in birds, early exposure to steroid hormones deposited in egg yolks is hypothesized to result in long-lasting effects on brain and behavior. however, the long-term effects of maternal androgens on the development of social behavior, and whether these could interfere with the effects of the endogenous gonadal hormones that mediate sexual differentiation, remain poorly known. to answer these questions, we enhanced yolk testosterone by injecting testosterone (t) in oil into japanese quail (coturnix ...201323123143
effects of meloxicam on hematologic and plasma biochemical analysis variables and results of histologic examination of tissue specimens of japanese quail (coturnix japonica).to determine the effects of meloxicam on values of hematologic and plasma biochemical analysis variables and results of histologic examination of tissue specimens of japanese quail (coturnix japonica).201223106456
fine mapping of complex traits in non-model species: using next generation sequencing and advanced intercross lines in japanese quail.as for other non-model species, genetic analyses in quail will benefit greatly from a higher marker density, now attainable thanks to the evolution of sequencing and genotyping technologies. our objective was to obtain the first genome wide panel of japanese quail snp (single nucleotide polymorphism) and to use it for the fine mapping of a qtl for a fear-related behaviour, namely tonic immobility, previously localized on coturnix japonica chromosome 1. to this aim, two reduced representations of ...201223066875
toxic interaction between fumonisin b1 and moniliformin for cardiac lesions in japanese quail.this study examined the effects of fumonisin b1 (fb1) and moniliformin (m) on the heart of japanese quail (coturnix coturnix japonica). three hundred and ninety day-old japanese quail were randomly divided into four groups: 1) fb1 alone (fx), 2) m alone (mx), 3) fb1 and m (fm), and 4) chick mash alone (cx). we used three pen replicates of 35 quail per pen in groups fx, mx, and fm and three pen replicates of 25 quail per pen in group cx. gross and microscopic changes in the heart were studied in ...201223050472
evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male japanese quails (coturnix coturnix japonica).the temporal expression of estrogen receptor (er)-α and er-β mrna was examined in male japanese quails. femurs of quails receiving 17β-estradiol underwent rt-pcr and histochemical analysis 1 to 15 days after treatment. untreated quails were used as controls (day 0). between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. expression of er-α was already observed on day 0 and increased slightly during bone formation whereas ...201223000578
quantification of interferon, interleukin, and toll-like receptor 7 mrna in quail splenocytes using real-time pcr.japanese quail (coturnix japonica) are farmed worldwide as poultry. quail have been used as experimental animals in various scientific fields, but their immunological characteristics have not been well characterized. in this study, to develop a method for analyzing the innate immune response of quail to infectious pathogens, we determined the nucleotide sequences of major interleukins (il) and toll-like receptor (tlr)-7 of quail and developed quantitative real-time pcr assays. the nucleotide seq ...201222991533
nicotine induces a conditioned place preference in male japanese quail (coturnix japonica).visual stimuli may play an important role in the development and maintenance of addiction in humans. research with a visually-oriented animal model such as japanese quail (coturnix japonica) may provide insight into how visual cues contribute to the addiction process. the aim of the current study was to investigate the rewarding properties of nicotine in male japanese quail using a biased conditioned place preference (cpp) procedure. adult male quail (n=30) were allowed to freely explore the ent ...201222939765
novel needle-in-straw vitrification can effectively preserve the follicle morphology, viability, and vascularization of ovarian tissue in japanese quail (coturnix japonica).cryopreservation of ovarian tissue has been the only effective way of ex situ conservation of female germplasm in avian species. a novel needle-in-straw (nis) vitrification method was developed to store tissue in straws instead of cryovials. fragments of ovarian tissue from one-week old japanese quail were transfixed on an acupuncture needle. they were immersed in equilibration and vitrification solutions containing dimethyl sulphoxide, ethylene glycol and sucrose. a layer of tin foil was rolled ...201222921390
age-related changes in mitochondrial ucp, ant and cox iii gene expression in the breast muscle of quails (coturnix coturnix japonica).efficient conversion of food into body mass has been associated with altered gene expression of some proteins of the electron transport chain. we evaluated the effect of age on mrna expression of uncoupling protein, adenine nucleotide translocase and cytochrome c oxidase complex iii in quails. one-day-old broiler quails were sacrificed after 7, 14, 21, or 28 days. six quails of each age were slaughtered by cervical dislocation. total rna was extracted from the breast muscle and reverse transcrib ...201222911582
pre- and post-natal stress in context: effects on the stress physiology in a precocial bird.developmental stress can significantly influence physiology and survival in many species. mammalian studies suggest that pre- and post-natal stress can have different effects (i.e. hyper- or hypo-responsiveness) on the hypothalamic-pituitary-adrenal (hpa) axis, the main mediator of the stress response. in mammals, the physiological intimacy between mother and offspring constrains the possibility to control, and therefore manipulate, maternal pre- and post-natal influences. here, using the japane ...201222899536
extinction of food-reinforced instrumental behavior in japanese quail (coturnix japonica).japanese quail (coturnix japonica) were reinforced with food for traversing a runway for either 18 or 36 trials, administered at a rate of 1 trial per day. then, all animals received 18 extinction trials. the latency to run from the start box to the goal box was the dependent variable. extinction was significantly slower in animals that had received 50% partial reinforcement during acquisition, whether relative to a group matched in terms of acquisition trials (36 trials, twice the number of rei ...201322866774
sperm proteasome degrades egg envelope glycoprotein zp1 during fertilization of japanese quail (coturnix japonica).at the time of fertilization, the extracellular matrix surrounding avian oocytes, termed the perivitelline membrane (pvm), is hydrolyzed by a sperm-borne protease, although the actual protease that is responsible for the digestion of the pvm remains to be identified. here, we show evidence that the ubiquitin-proteasome system is functional in the fertilization of japanese quail. the activities for the induction of the acrosome reaction and binding to zp3 as revealed by ligand blotting of purifie ...201222859519
interspecifc variation in eye shape and retinal topography in seven species of galliform bird (aves: galliformes: phasianidae).eye morphology and the retinal topography of animals that live in either 'open' (e.g., grassland) or 'enclosed' (e.g., forest) terrestrial habitats show common adaptations to constraints imposed by these different habitat types. although relationships between habitat and the visual system are well documented in most vertebrates, relatively few studies have examined this relationship in birds. here, we compare eye shape and retinal topography across seven species from the family phasianidae (gall ...201222806571
transgenic quail as a model for research in the avian nervous system: a comparative study of the auditory brainstem.research performed on transgenic animals has led to numerous advances in biological research. however, using traditional retroviral methods to generate transgenic avian research models has proved problematic. as a result, experiments aimed at genetic manipulations on birds have remained difficult for this popular research tool. recently, lentiviral methods have allowed the production of transgenic birds, including a transgenic japanese quail (coturnix coturnix japonica) line showing neuronal spe ...201322806400
effects of sulfamethazine on induction of precocious puberty in japanese quails (coturnix japonica) assessed through monitoring the hormonal changes and gonadal development.to investigate the effect of dietary supplementation of sulfamethazine (smz) on growth performance, gonadal development and hormonal changes, male and female japanese quails (coturnix japonica) were fed a control diet with or without smz (0.2%) from one day post hatching until 6 weeks of age. in male quail, the deviation in growth performance between smz and control chicks started at the 3rd week, and the disparity was significant at the 5th and 6th weeks. hormonal analysis revealed a substantia ...201222785325
effect of dietary l-ascorbic acid (l-aa) on production performance, egg quality traits and fertility in japanese quail (coturnix japonica) at low ambient temperature.environmental stress boosts the levels of stress hormones and accelerates energy expenditure which subsequently imbalance the body's homeostasis. l-ascorbic acid (l-aa) has been recognized to mitigate the negative impact of environmental stress on production performances in birds. the present investigation was carried out to elucidate the effect of different dietary levels of l-aa on production performance, egg quality traits and fertility in japanese quail at low ambient temperature. sixty matu ...201225049657
factors influencing the turnover and net isotopic discrimination of hydrogen isotopes in proteinaceous tissue: experimental results using japanese quail.stable hydrogen isotopes (δ(2)h) are commonly used in studies of animal movement. tissue that is metabolically inactive after growth (e.g., feathers) provides spatial or dietary information that reflects only the period of tissue growth, whereas tissues that are metabolically active (e.g., red blood cells) provide a moving window of forensic information. however, using δ(2)h for studies of animal movement relies on the assumption that tissue δ(2)h values reflect dietary δ(2)h values, plus or min ...201322705487
negative allometry of docosahexaenoic acid in the fowl lung and pulmonary surfactant phospholipids.in a recent study (comp. biochem. physiol. b. (2010)155: 301-308) we reported that the fatty acids (fa) of the avian (7 species) total lung phospholipids (pl) (i.e. lung parenchyma and surfactant together) provide allometric properties. to test whether this allometric scaling also occurs in either of the above components, in six gallinaceous species, in a body weight range from 150 g (japanese quail, coturnix coturnix japonica) to 19 kg (turkey, meleagris gallopavo) the pl fa composition (mol%) ...201222695520
differential mrna expression of seven genes involved in cholesterol metabolism and transport in the liver of atherosclerosis-susceptible and -resistant japanese quail strains.two atherosclerosis-susceptible and -resistant japanese quail (coturnix japonica) strains obtained by divergent selection are commonly used as models to study atherosclerosis, but no genetic characterization of their phenotypic differences has been reported so far. our objective was to examine possible differences in the expression of genes involved in cholesterol metabolism and transport in the liver between these two strains and to evaluate the value of this model to analyze the gene system af ...201222682430
melatonin affects the temporal pattern of vocal signatures in birds.in humans and other animals, melatonin is involved in the control of circadian biological rhythms. here, we show that melatonin affects the temporal pattern of behavioral sequences in a noncircadian manner. the zebra finch (taeniopygia guttata) song and the crow of the japanese quail (coturnix japonica) are courtship vocalizations composed of a stereotyped sequence of syllables. the zebra finch song is learned from conspecifics during infancy, whereas the japanese quail crow develops normally wi ...201222506964
sodium levels for japanese quail at initial phases.the aim of this research was to determine the nutritional requirements of sodium for japanese quail (coturnix coturnix japonica) during the periods of 1 to 21 d and 22 to 40 d of age, as well as to evaluate the residual effect on egg production and densitometry bone traits from 41 to 63 d. two experiments were developed. experiment 1: 360 japanese quail were used, from 1 to 21 d of age. treatments consisted of 5 sodium levels (0.06, 0.12, 0.18, 0.24, and 0.30%). experiment 2: 240 japanese quail ...201222499870
quantitative hplc of pigments of irregularly coloured eggshells: application to aliquots of powdered shell from quail.twelve quail eggshells from farmed coturnix coturnix japonica were separately ground to fine powder and two aliquots of each (average weights 13.86 mg and 51.90 mg) were extracted with formic acid. biliverdin (38-284 pmol/mg) and protoporphyrin (841-1666 pmol/mg) were measured by hplc. there was good agreement between the values for the corresponding samples and with those for two entire eggshells from the same source. the preparation of a homogenate as a powder from heterogeneously pigmented eg ...201222495788
in ovo exposure to nonylphenol and bisphenol a resulted in dose-independent feminization of male gonads in japanese quail (coturnix japonica) embryos.sex reversal effects of nonylphenol and bisphenol a on the gonads in f(1) (awe × we) japanese quail (coturnix japonica) embryos were investigated using an in vivo screening model developed previously. the f(1) (awe × we) japanese quail are a useful avian model because sex differentiation is confirmed by the plumage color before hatching, ruled by a criss-cross inheritance. the nonylphenol at 200, 2,000, 20,000, and 200,000 ng/egg and bisphenol a at 20, 200, 2,000, and 20,000 ng/egg were injected ...201222447559
melamine residues in eggs of quails fed on diets containing different levels of melamine.1. an experiment was conducted to determine residual melamine concentrations in eggs of quail given diets containing different concentrations of melamine, and to estimate the time at which withdrawing melamine from the diet would result in undetectable concentrations in eggs. a total of 600 japanese quails (coturnix coturnix japonica) were randomly divided into 5 treatments, and given melamine at concentrations of 0, 2, 10, 50, and 100 mg/kg diet respectively for 30 d. from d 31 to 41, all quail ...201222404806
effects of propolis on eggshell microbial activity, hatchability, and chick performance in japanese quail (coturnix coturnix japonica) eggs.propolis is a sticky resin produced by worker honeybees from substances collected from plants, and it has strong antibacterial and antifungal properties. the purpose of this study was to establish the effects of propolis on egg weight loss, hatchability, chick performance, and to control microbial activity naturally occurring on eggshells. a total of 750 fresh eggs was randomly divided into 5 groups. eggs from the first group were sprayed with ethyl alcohol (70%, a), the second group was sprayed ...201222399742
effects of ultrasonic treatment on eggshell microbial activity, hatchability, tibia mineral content, and chick performance in japanese quail (coturnix coturnix japonica) eggs.the purpose of this study was to establish the effects of ultrasonic treatment of eggs on egg weight loss, hatchability, tibia mineral content, chick performance, and eggshell microbial activity. a total of 600 fresh eggs was randomly divided into 5 groups. treatments were no ultrasound, 35 khz for 5 min, 35 khz for 10 min, 35 khz for 15 min, and eggs sprayed with benzalkonium chloride solution at 0.02% (negative control). the eggshell microbial activity was reduced significantly at all ultrason ...201222334750
age, photoperiod and estrogen dependent variations in the shell gland and the expression of avt in the ovary of japanese quail.present work was undertaken to describe (i) age dependent (prepuberal-3, 4, 5 and 6 weeks old, puberal and actively laying 8 and 12 weeks old and aged 78 weeks old) (ii) photoperiodic response dependent (photosensitive and photorefractory) and sex steroid dependent (estradiol benzoate and its antagonist tamoxifen treated) variation in the ovary and shell gland activity of japanese quail (coturnix coturnix japonica). further, in view of the role of neurohypophysial peptide arginine vasotocin (avt ...201222310162
sequence and in vitro function of chicken, ring-necked pheasant, and japanese quail ahr1 predict in vivo sensitivity to dioxins.there are large differences in sensitivity to the toxic and biochemical effects of dioxins and dioxin-like compounds (dlcs) among vertebrates. previously, we demonstrated that the difference in sensitivity between domestic chicken (gallus gallus domesticus) and common tern (sterna hirundo) to aryl hydrocarbon receptor 1 (ahr1)-dependent changes in gene expression following exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) is based upon the identities of the amino acids at two sites within t ...201222296185
the role of the male cloacal gland in reproductive success in japanese quail (coturnix japonica).the adult male japanese quail has a well developed cloacal gland that produces meringue-like white foam. the physiological significance of the cloacal gland and its foam is still unclear. therefore, we conducted two experiments to establish the functional role of the cloacal gland and its foam in natural mating and oviducal sperm transport. in the first experiment, artificial insemination of equal numbers of spermatozoa diluted in foam extract and normal saline once in a week were used to determ ...201222281087
carotenoid supplementation and gnrh challenges influence female endocrine physiology, immune function, and egg-yolk characteristics in japanese quail (coturnix japonica).androgens and carotenoids circulating in plasma affect the physiology and behavior of vertebrates. much is known about control mechanisms and functions of each of these substances, yet their interactive effects are not well understood. here we examine possible additive, multiplicative, and interactive effects of testosterone and carotenoids on female endocrine physiology, immunocompetence, and investment in eggs by simultaneously manipulating levels of testosterone [via gonadotropin releasing ho ...201222237302
induction of cytochrome p4501a by highly purified hexachlorobenzene in primary cultures of ring-necked pheasant and japanese quail embryo hepatocytes.primary cultures of ring-necked pheasant (phasianus colchicus) and japanese quail (coturnix japonica) embryo hepatocytes were used to compare the potencies of highly purified hexachlorobenzne (hcb-p), reagent-grade hcb (rg-hcb) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) as inducers of ethoxyresorufin o-deethylase (erod) activity, cytochrome p4501a (cyp1a4) messenger ribonucleic acid (mrna) and cyp1a5 mrna. hcb-p, rg-hcb and tcdd all induced erod activity and up-regulated cyp1a4 and cyp1a5 mr ...201222227438
changes in plasma gonadotrophins, testosterone, prolactin, thyroxine and triiodothyronine concentrations in male japanese quail (coturnix coturnix japonica) of a heavy body weight line during photo-induced testicular growth and regression.1. simultaneous changes of cloacal gland area (cga) and plasma luteinising hormone (lh), follicle stimulating hormone (fsh), testosterone (t), prolactin (prl), thyroxine (t(4)) and triiodothyronine (t(3)) during photo-induced testicular growth and regression were measured in commercially bred japanese quail from a heavy body weight line. 2. somatically mature male japanese quail were transferred from short days (light:dark 8l:16d) at 10°c, to long days (16l:8d) at 20°c; and sexually mature male ...201122221245
phytosterol additive boosts adrenal response to acth in male japanese quail (coturnix coturnix japonica).to investigate the adrenal effect of a phytosterol (ps) additive, 80 male japanese quail were divided into four sub-groups and fed 0, 40, 400, and 4,000 ppm of ps, respectively, for 21 days. subsequently, 50% of the birds from each dosage group were subjected to a 6-day adrenal function test, whereby they were injected with long-lasting adrenocorticotropin (acth). the remaining quail in each ps dosage group were raised under normal conditions. the groups receiving 400 and 4000 ppm ps exhibited d ...201222212440
subulura brumpti infection - an outbreak in japanese quails (coturnix coturnix japonica).subulura brumpti infection was observed in japanese quails and caused heavy production loss up to 10%. gross lesions were confined to caecum of the affected birds. pathological changes suggestive of acute cecal hemorrhagic enteritis were recorded. closer observation of the cecal loop revealed wavy movement with thousands of tiny worms. based on morphometry, the worms were identified as s. brumpti. condition was responded to the albendazole treatment efficiently and all the birds were recovered a ...201225653749
allometric growth of testes in relation to age, body weight and selected blood parameters in male japanese quail (coturnix japonica).the japanese quail is a very valuable animal model for research in a variety of biological disciplines. the purpose of this study was to characterize and interrelate age-dependent testicular parameters with various blood constituents: blood glucose, plasma proteins and packed cell volume that are developing concurrently in the growing bird. another objective of the study was to identify selective physioanatomical markers for predicting the testicular growth and the onset of sexual maturity. male ...201225243007
spatial orientation in japanese quails (coturnix coturnix japonica).finding a given location can be based on a variety of strategies, for example on the estimation of spatial relations between landmarks, called spatial orientation. in galliform birds, spatial orientation has been demonstrated convincingly in very young domestic chicks. we wanted to know whether adult japanese quails (coturnix coturnix japonica) without food deprivation are also able to use spatial orientation. the quails had to learn the relation of a food location with four conspicuous landmark ...201122163284
an immunohistochemical study of ovarian follicle histogenesis in the early post-hatch japanese quail (coturnix coturnix japonica).the early post-hatch development of immunoreactivity to vimentin, desmin, smooth muscle actin (sma) and laminin, in relation to follicle histogenesis, was described in this study. ovigerous cords in day old quails contained pre-granulosa cells and oocytes. pre-granulosa cells at the cortico-medullary junction were vimentin immunopositive. a laminin immunopositive basement membrane and desmin immunopositive mesenchymal cells lined the ovigerous cords. ovigerous cords in 3-day-old quails contained ...201221919950
acute stress differentially affects aromatase activity in specific brain nuclei of adult male and female quail.the rapid and temporary suppression of reproductive behavior is often assumed to be an important feature of the adaptive acute stress response. however, how this suppression operates at the mechanistic level is poorly understood. the enzyme aromatase converts testosterone to estradiol in the brain to activate reproductive behavior in male japanese quail (coturnix japonica). the discovery of rapid and reversible modification of aromatase activity (aa) provides a potential mechanism for fast, stre ...201121878510
assembly of the inner perivitelline layer, a homolog of the mammalian zona pellucida: an immunohistochemical and ultrastructural study.the avian inner perivitelline layer (ipvl), a homologous structure to the mammalian zona pellucida, is deposited between the granulosa cells and the oocyte cell membrane during folliculogenesis. the glycoprotein meshwork of the ipvl forms a 3-dimensional matrix and possesses important functions in the fertilization process: it contributes to the binding of avian spermatozoa to the oocyte and induces acrosomal exocytosis. in contrast to the zona pellucida of mammals, the ipvl does not prevent the ...201221778679
adrenocortical response to mating, social interaction and restraint in the female japanese quail.in some species, the act of mating may be the only occasion when a female judges the male and the course of the sexual encounter might influence forms of maternal investment that are mediated by hormonal pathways. despite the growing interest in fitness consequences of acute adrenocortical responses, it has not been determined to what extent the act of mating provokes such responses in females. we used female japanese quail (coturnix japonica) to determine to what extent any increase in corticos ...201121763334
oocytic expression of zona pellucida protein zp4 in japanese quail (coturnix japonica).the avian perivitelline layer, an extracellular matrix homologous to the zona pellucida (zp) of mammalian oocytes, is composed mainly by zona pellucida gene family glycoproteins. our previous studies in japanese quail have demonstrated that the matrix's components, zp3 and zpd, are synthesized in ovarian granulosa cells. another component, zp1, is synthesized in the liver. recently, we demonstrated that another minor constituent, zp2 is produced in the oocytes of the immature follicles. in the p ...201121729200
temporal pattern of locomotor activity recuperation after administration of propofol in japanese quail (coturnix coturnix japonica).the present study evaluates the organization and complexity of the temporal pattern of locomotion after an acute administration of propofol in japanese quail by using traditional and fractal analysis. birds were administered with propofol 0, 10, 20, 40 or 80 mg/kg. ten min after administration, they were placed in an open-field apparatus and their locomotor activity was recorded during 45 min at a resolution of 0.5 s. a significant dose dependant increase in the latency to initiate ambulation wa ...201221724216
isolation and characterization of free haem from the shell gland of quail and hen.free haem was isolated from the shell gland of the quail, coturnix coturnix japonica, and of the fowl, galinus domesticus, and characterized by hplc-esi-ms/ms. quantification by hplc gave values of 1.17-1.40 nmol/mg quail shell gland protein for haem, 1.66-2.17 nmol/mg protein for protoporphyrin and 0.25-0.40 nmol/mg protein for biliverdin. possible implications of this previously unreported finding are discussed but they are not considered incompatible with the conclusion that all eggshell pigm ...201221678459
cholesterol content and intramuscular collagen properties of pectoralis superficialis muscle of quail from different genetic groups.to study growth performance and meat quality traits (cholesterol content and intramuscular collagen properties) of quail, 3 trials were carried out. trial 1 used males of generation 19 of the egg type japanese quail (coturnix japonica) selected previously (until generation 17) for low (n = 8) or high (n = 7) yolk cholesterol content as well as an unselected control (n = 11). trial 2 used males of meat pharaoh quail selected earlier (generations 1 to 6 and 9 to 11) on the basis of bw decrease aft ...201121673181
sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in japanese quail (coturnix japonica).an antibody library against quail sperm plasma membrane components was established and a mab, which strongly inhibits sperm perforations of the perivitelline membrane (pvm) was obtained from the library. the antigen molecule of the mab showed an apparent molecular weight of 45  kda, and was distributed both on the surface and in the acrosomal matrix of the sperm head. periodate oxidation revealed that the epitope of the antigen includes a sugar moiety. tandem mass spectrometry analysis of the an ...201121642383
adjustments of the oxygen diffusing capacity to energetic demands during the development of the quail (coturnix coturnix japonica).one of the hypotheses that attempt to explain physiological limitations of energy budgets is the symmorphosis hypothesis, which proposes that if matching structures to functional needs were combined with the strict economy of energy and materials, the result would be an optimal organ design for the specific function it serves. evidence in favor of symmorphosis in adults is as abundant as evidence against it, but the plasticity of some morphological traits may be dependent on the ontogenetic stag ...201121496493
genetic parameters of slaughter and carcase traits in japanese quail (coturnix coturnix japonica).1. genetic parameters of slaughter and carcase traits were estimated for 5-week-old japanese quail reared under the one-to-one sire and dam pedigree recorded system. data were analysed using restricted maximum likelihood (reml) with relationship matrix. records of 1244 animals from 119 sires and 156 dams were analysed. 2. an individual animal model was used which had the animals' genetic effect as the only random factor. heritability and standard errors of slaughter, cold carcase, breast, leg, w ...201121491239
developmental toxicity of glyceryl trinitrate in quail embryos.although glyceryl trinitrate (gtn) is used extensively to treat angina and heart failure, little is known about its effects on the conceptus during organogenesis. the goal of these studies was to investigate the effects of gtn in a model organism, the quail (coturnix coturnix japonica) embryo.201121472843
the ossification of the pectoral girdle and wing skeleton of the quail (coturnix coturnix japonica).the onset of ossification centres of the pectoral girdle and wing skeleton of the quail (coturnix coturnix japonica) has been studied in embryos and juvenile birds, which were sampled daily from the 4th day of incubation up to the 12th day after hatching. specimens, which were cleared and were stained with alcian blue and alizarin red s, were examined at the stereomicroscope. the furcula ossified with intramembranous ossification at the 7th day of incubation. the first centres of perichondral os ...201121392085
dietary fatty acid composition influences tissue lipid profiles and regulation of body temperature in japanese quail.many avian species reduce their body temperature (t(b)) to conserve energy during periods of inactivity, and we recently characterized how ambient temperature (t(a)) and nutritional stress interact with one another to influence physiologically controlled hypothermic responses in japanese quail (coturnix japonica). in the present study, we examined how the fatty acid (fa) composition of the diet influences the fa composition of phospholipids in major organs and how these affect controlled hypothe ...201121328066
endocrine disrupting effects of low dose 17 β-estradiol (e2) on the japanese quail (coturnix japonica) were detected by modified one-generation reproduction study.previously, we investigated endocrine disrupting effects of 17 β-estradiol (e(2)) on japanese quail (coturnix japonica) in the avian reproduction test according to the testing guidelines, in which new endpoints such as blood vitellogenin (vtg) concentration in parent quails and pathology of f(1) chicks were added, and consequently these additional endpoints suggested to be sensitive markers for detecting any impacts of endocrine disrupting effects (shibuya et al., 2005b). in the present study, t ...201121297340
deleterious effects of molting on the morpho-physiology of japanese quail layers (coturnix japonica).molting is a natural physiological phenomenon involving the periodic replacement of old feathers with new ones in the avian species. during mid-november an extensive loss of feathers in japanese quail was observed in our breeding colony. the cause of molting could not be established, however, lower ambient temperatures may have played a major role and the decrease in day length could not be ruled out as a contributing factor. this study was conducted to correlate some aspects of the molting proc ...201125285099
[lampbrush chromosomes of the japanese quail (coturnix coturnix japonica): a new version of cytogenetic maps].avian oocyte chromosomes are transfomed into giant transcriptionally active lampbrush chromosomes (lbcs) at meiosis 1 diplotene. these chromosomes are a convenient tool for high-resulution cytogenetic analysis. using differential staining with fluorochromes dapi and cma3, we have constructed detailed cytological maps for lampbrush macrochromosomes 1-5 and zw of the japanese quail coturnix coturnix japonica. we also performed a comparative analysis ofmitotic chromosomes and lbcs corresponding to ...201021254550
the influence of inclusions of vitamin e and corn oil on semen traits of japanese quail (coturnix coturnix japonica).reported was an investigation of the effect of vitamin e (vit.e) and corn oil on semen traits of male japanese quail (coturnix coturnix japonica). from 8 to 20 wk of age, birds were raised on corn-based diets supplemented with corn oil (0 and 3%) and vit.e (national research council (nrc) recommended 25mg/kg/day/dry matter and 150 mg/kg/day/dry matter) in a 2×2 factorial manner. the diet was supplemented with corn oil and vit.e (e2c2) which provided additional n-6 polyunsaturated fatty acids in ...201121177050
hypocholesterolemic effect of karaya saponin in japanese laying quails (coturnix coturnix japonica).the effect of karaya saponin supplementation on the serum and egg yolk cholesterol and fatty acid composition in egg yolk were investigated in japanese quails. a total of 80 japanese quails aged 5 weeks were equally divided into four groups of 20. four levels (0, 25, 50 and 75 mg/kg feed) of karaya saponin were included in the basal diet and experiment was lasted for 6 weeks. the cholesterol fractions in the egg yolk and serum were measured by enzymatic assay, and the fatty acid composition in e ...201121114549
the effect of different foam concentrations on sperm motility in japanese quail.a study was conducted to determine the effect of foam extract on sperm motility in the male japanese quail (coturnix coturnix japonica). adult male quails (<12 weeks) of heavy body weight strain were housed in individual cages and divided into 5 groups according to the size of their cloacal glands. the data indicated that the size of the cloacal gland was positively correlated with the frequency of foam secretion and total foam production. one gram of freshly collected clean foam was mixed with ...201021076546
characterization of lactate dehydrogenase enzyme in seminal plasma of japanese quail (coturnix coturnix japonica).lactate dehydrogenase enzyme present in quail seminal plasma has been characterized. polyacrylamide gel electrophoresis and subsequently with ldh specific staining of seminal plasma revealed a single isozyme in quail semen. studies on substrate inhibition, ph for optimum activity and inhibitor (urea) indicated the isozyme present in the quail semen has catalytic properties like ldh-1 viz. h-type. furthermore, unlike other mammalian species, electrophoretic and kinetic investigations did not supp ...201121074838
evaluation of the impact of in-ovo exposure to dicofol on avian reproduction.to assess the possible impact of the currently used organochlorine insecticide, dicofol, on the development and reproduction of avian species, in ovo exposure experiments to its p,p' and o,p' isomers were performed using japanese quail (coturnix japonica) eggs. o,p'-dicofol (0.3-100 µg/g of egg) and p,p'-dicofol (3-100 µg/g) were injected into the yolk prior to incubation and hatched chicks were raised to adulthood. in ovo treatment with o,p'-dicofol impaired the eggshell-forming ability of fema ...201020872696
toxico-pathological effects of sodium hypochlorite administration through drinking water in female japanese quail (coturnix japonica).fifty female japanese quail (coturnix japonica) at 40 days were divided into five equal groups. sodium hypochlorite was added in drinking water to provide 0, 25, 50, 200, 400 mg chlorine/l for 6 weeks. at week 7, the chlorine concentration of 25 and 50 mg groups was increased to 100 mg and further increased to 400, 1600 and 6400 mg at weeks 8, 9 and 10, respectively. at the end the experiment (week 10), all the birds were sacrificed. no clinical signs were observed in quail given 50 mg chlorine/ ...201020729259
pathological and genotoxic effects of atrazine in male japanese quail (coturnix japonica).the objective of the present study was to determine the pathological and genotoxic effects of atrazine (atz) in male japanese quail. adult male japanese quail were administered atz daily at 0-500 mg/kg bw (a-h groups) orally for 45 days. the blood and morbid tissues were collected at day 15, 30, and 45 of the treatment. a significant decrease in feed intake, body weight, erythrocyte counts, hemoglobin and hematocrit values were observed at high atz dose compared to control. leukocyte counts decr ...201120607394
distribution, density and histochemical profiles of the lung mast cells during the post-hatching period of japanese quails (coturnix coturnix japonica).the distribution, density and histochemical characteristics of mast cells in the lungs of the japanese quail were investigated during the post-hatching period. in the period starting from the first to the 60th day post-hatching, based on proteoglycan content, three types of mast cells, which were alcian blue-positive, safranin o-positive and alcian blue/safranin o-positive, were found to exist in the lungs. the application of staining with berberine sulphate demonstrated that, similar to the dis ...201120537669
light microscopic study on eimeria species infecting japanese quails reared in saudi arabian farms.japanese quails coturnix coturnix japonica reared in economic farms were individually investigated for coccidian infections. the results indicated the absence of infections in birds younger than 1 month. an eimeria infection rate of up to 80% was detected in birds 7-9 weeks old with a general infection rate of 29%. the infection rate decreased to 21.42% in birds older than 10 weeks. morphometric characteristics of freshly shed, unsporulated oocysts were taken. these oocysts appeared pale yellow ...201020422217
production of donor-derived offspring from cryopreserved ovarian tissue in japanese quail (coturnix japonica).cryopreservation of avian ova and embryos is challenging because of the yolky structure of the egg. as an alternative, with the development of effective cryopreservation protocols, ovarian tissue cryopreservation could be used for cryobanking for birds. pieces of ovarian tissue of week-old japanese quail (coturnix japonica) were frozen at 0.5 degrees c/min in a programmable freezer or vitrified by immersion in liquid nitrogen. straws containing slow-frozen samples were thawed in ice water, and v ...201020237335
reevaluation of the developmental toxicity of dieldrin by the use of fertilized japanese quail eggs.to reevaluate the toxicity of the organochlorine insecticide and persistent organic pollutant dieldrin and confirm its impact on development, an exposure trial using bird eggs was performed. dieldrin at concentrations of 10-100 microg/g of egg was injected into the yolks of japanese quail (coturnix japonica) eggs. hatchlings from the eggs were raised to sexual maturity and multiple tests to detect the harmful effects of dieldrin were conducted. dieldrin at 100 microg/g decreased egg hatchability ...201020211759
expression of calcium-sensing receptor in quail granulosa explants: a key to survival during folliculogenesis.this study investigated the potential role of the calcium-sensing receptor (car) in mediating survival of granulosa cells (gcs) in follicles of the japanese quail (coturnix coturnix japonica). immunoreactivity of car was shown in gcs of quail preovulatory follicles as well as in the remnants of the gc layer after ovulation. conversely, the car could not be detected by immunocytochemistry in the granulosa of smaller undifferentiated follicles. the presence of car in follicles destined to ovulate ...201020186967
fasting triggers hypothermia, and ambient temperature modulates its depth in japanese quail coturnix japonica.we tested three hypotheses regarding the cues that elicit facultative hypothermia in japanese quail (coturnix japonica): h(1)) ambient temperature (t(a)), alone, influences the onset and depth of hypothermia; h(2)) fasting, alone, influences the onset and depth of hypothermia; h(3)) t(a) acts synergistically with fasting to shape the use of hypothermia. eight quail were maintained within their thermoneutral zone (tnz) at 32.6+/-0.2 degrees c, and eight below their lower critical temperature (t(l ...201020060055
effects on differentiation of reproductive organs and sexual behaviour in japanese quail by excessive embryonic eralpha activation.exposure of japanese quail (coturnix japonica) embryos to oestrogenic substances disrupts sexual differentiation of the reproductive tract of both sexes and impairs the copulatory behaviour of the adult male. to examine whether these effects can be induced by selective activation of oestrogen receptor alpha (eralpha), japanese quail eggs were injected with various doses of the selective eralpha agonist 16alpha-lactone-oestradiol (16alpha-le(2)). the natural oestrogen 17beta-oestradiol (e(2)) was ...201020047727
pharmacokinetics of florfenicol in the plasma of japanese quail.to determine the pharmacokinetics and bioavailability of florfenicol in the plasma of healthy japanese quail (coturnix japonica).200919966901
effects of 25-hydroxycholecalciferol and soy isoflavones supplementation on bone mineralisation of quail.1. the effects of 25-hydroxycholecalciferol (25-oh-d(3)) and soy isoflavones supplementation on performance, carcase recovery, bone mineral density, and tibia ash, ca, p, and serum vitamin d concentrations and alkaline phosphatase activity in the japanese quail (coturnix coturnix japonica) exposed to high ambient temperature were evaluated. 2. a total of 270 ten-d-old japanese quail were randomly assigned to 9 treatment groups, 6 replicates of 5 birds each in a 2 x 3 x 3 factorial arrangement of ...200919946824
the crystal polymorphism of calcium carbonate is determined by the matrix structure in quail eggs.two calcified structures, the eggshell and sperm-associated body (sb), are present in the eggs of the japanese quail, coturnix japonica. x-ray diffractometry showed that calcium carbonates take the form of calcite in the eggshell and aragonite in the sb. the aim of the present study was to identify the factors that determine the morphology of calcium carbonate crystals. the matrix of edta-treated eggshell was a meshwork of vesicles, 200 to 500 nm in diameter, connected by fine fibers or fibrous ...200919903967
a possible role of chorion protease in shell membrane degradation during development of quail embryos.in the eggs of the quail coturnix japonica, the limiting membrane demarcates the shell membrane at the interface with the albumen and decreases in width during the hatching process. this study was done to identify agents that affect the width of this limiting membrane. zymography tests on extracts from extraembryonic tissues, yolk sacs, or chorioallantoic membranes, or all three, showed proteolytic activities during d 4 to 10 of incubation. localization experiments on these activities, performed ...200919903963
effect of oral cadmium and zinc application on leukocytes biological activity in japanese quails (coturnix coturnix japonica).objective of this study was to investigate changes in biological activity of blood leukocytes in japanese quails, caused by zinc and cadmium administration. four groups of japanese quails were used. three experimental groups of quails were exposed either to cd (0.12 mg cd/quail), zn (4 mg zn/quail) or a combination of cd and zn (0.12 mg cd, 4 mg zn/quail), which were added daily to the drinking water. the fourth group was the control group. the metabolic activity of phagocytes and mitogenic acti ...201319863008
zona pellucida protein zp2 is expressed in the oocyte of japanese quail (coturnix japonica).the avian perivitelline layer (pl), a vestment homologous to the zona pellucida (zp) of mammalian oocytes, is composed of at least three glycoproteins. our previous studies have demonstrated that the matrix's components, zp3 and zpd, are synthesized in ovarian granulosa cells. another component, zp1, is synthesized in the liver and is transported to the ovary by blood circulation. in this study, we report the isolation of cdna encoding quail zp2 and its expression in the female bird. by rnase pr ...201019846483
fibroblast growth factor-2 and vascular endothelial growth factor expression in the ileocecal region of quail (coturnix coturnix japonica).we undertook this study to immunolocalize in quail vascular endothelial growth factor (vegf) and fibroblast growth factor (fgf-2) in the ileocecal region, which is a significant entry point for intestinal immunity. diffuse cytoplasmic reaction for fgf-2 and vegf was observed in the epithelial cells of the distal ileum and proximal cecal mucosa. vegf immunoreactive cells, which give strong intracytoplasmic immunoreaction, were observed in the lamina propria of both intestinal parts. fgf-2 immunor ...201019824877
genistein supplementation to the quail: effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels.genistein, a soy phytoestrogen, is a powerful antioxidant. in the present study, we investigated the effects of dietary genistein supplementation on japanese quail (coturnix coturnix japonica) laying performance and egg yolk contents of malondialdehyde (mda), vitamin a, and vitamin e. malondialdehyde is an indicator of lipid peroxidation, whereas vitamins a and e have antioxidant properties. birds (n=150; 5 wk old) were randomly assigned to 1 of 3 groups consisting of 50 birds (5 replicates of 1 ...200919762866
the effects of testosterone on immune function in quail selected for divergent plasma corticosterone response.the immunocompetence handicap hypothesis (ichh) suggests that the male sex hormone testosterone has a dual effect; it controls the development and expression of male sexually selected signals, and it suppresses the immune system. therefore only high quality males are able to fully express secondary sexual traits because only they can tolerate the immunosuppressive qualities of testosterone. a modified version of the ichh suggests that testosterone causes immunosuppression indirectly by increasin ...200919749105
cuticle formation in quail eggs.the present study was conducted to determine both the site at which cuticle materials are produced and the critical period for their production in the oviductal uterus of the japanese quail, coturnix japonica. an antiserum was produced against the 32-kda band in electrophoretic profiles of cuticle materials obtained from eggshells decalcified with edta. immunofluorescence and immunoelectron microscopic observations revealed that the 32-kda protein was synthesized in luminal ciliated epithelial c ...200919663645
structure of the oesophagus and morphometric, histochemical-immunohistochemical profiles of the oesophageal gland during the post-hatching period of japanese quails (coturnix coturnix japonica).in the oesophagus, mucins, which originate from oesophageal submucosal glands, play an important role in the mucosal protection as a pre-epithelial barrier. in this study, the structure of cervical and thoracic parts of oesophagus of japanese quail during the post-hatching period was compared, and the contents of carbohydrate and gastric mucin muc5ac of the oesophageal glands in these parts were analysed at the light microscope levels by applying conventional histochemical and immunohistochemica ...200919650777
visual asymmetries in japanese quail (coturnix japonica) retain a lifelong potential for plasticity.adult japanese quail display left-eye/right-hemisphere dominance in visually guided sexual tracking. in 2 experiments, the authors set out to answer if this functional cerebral asymmetry is modifiable by posthatch monocular deprivation. in experiment 1, the left or the right eye of 2-day old quail were closed for 70 days. quail were run in a left- or a right-turning runway to obtain access to a conspecific of the opposite sex. the performance of both left and right eye systems was equal. in expe ...200919634940
ultrastructure and lectin cytochemistry of secretory cells in lingual glands of the japanese quail (coturnix coturnix japonica).in the present study, as continuation of our previous research, japanese quail (coturnix coturnix japonica) lingual glands were investigated by means of transmission electron microscopy (tem) to understand the cytoarchitecture and the subcellular sugar distribution within the different secretory structures. indeed, glycosidic residues were visualized by applying an indirect technique of binding and the terminal sialoglycoconjugate sequences were characterized by employing sialidase digestion com ...200919609855
hormones and sexual differentiation of avian social behavior.research on the hormonal basis of sexual differentiation of mammalian behavior and its neural substrates constitutes an important chapter in the study of neural development that has led to important insights into how to conceptualize sexuality. here research on sexual differentiation of avian social behavior is discussed with an eye toward potential insights into avian sexuality. hormone manipulation experiments with japanese quail (coturnix japonica) and zebra finches (taeniopygia guttata) reve ...200919546571
learning enhances female control over reproductive investment in the japanese quail.the adaptive significance of learning is supported by studies showing its positive effects on mating behaviour, but they rarely go beyond fertilization success. here we studied how learning contributes to qualitative reproductive investment, by testing the hypothesis that mating in the context that predicts male appearance has positive effects on female reproductive investment compared with unsignalled mating. using japanese quail (coturnix japonica), we found that effects of mating in the conte ...200919535375
age-dependent expression of avt and its oxytocic-like receptor vt3 in the shell gland of japanese quail, coturnix coturnix japonica.the avian neurohypophyseal hormone avt is an important regulatory hormone involved in many physiological processes including oviposition; an age-related phenomenon, through its action on the shell gland. in this study, immunohistochemistry and in situ hybridization was performed to study the expression of immunoreactive (ir) avt and its oxytocic-like receptor vt3 transcript in the ovary/shell gland of japanese quail representing sexually immature, mature and old condition. our results indicate t ...201019505466
organizational effects of dde on brain vasotocin system in male japanese quail.p,p'-dde, or ethylene, 1,1-dichloro-2,2-bis(p-chlorophenyl), is the main metabolite of the pesticide ddt, or 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane. it is an androgen receptor antagonist and testosterone hydroxylase modulator that is also more persistent than its parent compound. in a previous study we demonstrated that embryonic exposure to different doses of p,p'-dde accelerated onset of puberty in females and reduced male reproductive behavior. in the present study we investigated the ...200919442834
the effects of vitamin c and e supplementation on heat shock protein 70 response of ovary and brain in heat-stressed quail.1. the effects of vitamin c (l-ascorbic acid) and vitamin e (alpha-tocopherol acetate) supplementation on egg production and heat shock protein 70 (hsp70) response of ovary and brain in the japanese quail (coturnix coturnix japonica) exposed to high ambient temperature were evaluated. 2. the birds (n = 540; 55 d old) were randomly assigned to 18 groups consisting of 3 replicates of 10 birds each in a 2 x 3 x 3 factorial arrangement of treatments. birds were kept in wire cages in a temperature-co ...200919373727
sensitive embryonic endpoints with in ovo treatment for detecting androgenic and anti-androgenic effects of chemicals in japanese quail (coturnix japonica).the aim of the current study was to establish the sensitive embryonic endpoints and a test system for detecting androgenic and anti-androgenic potential of chemicals using an in ovo treatment assay in japanese quail. in ovo injection with 0 to 75 microg of cyproterone acetate (ca) was performed on d 12 of incubation, followed by 0 to 300 microg of testosterone propionate (tp) injection on d 13 and histological examination on d 16. experimental groups were composed of control (twice injected corn ...200919359695
tolerance of japanese quail embryos and young chicks to hypothermia.the purpose of this study was to explore the tolerance of japanese quail (coturnix coturnix japonica) embryos and young quail chicks to a very low ambient temperature. fifty (n = 50) quail embryos at age embryonic age d 6 were placed in a cold room at 13 degrees c for 24 h. the heart rate (hr) was determined with an infrared hr sensor in an instrument called "buddy." after 12 and 24 h, the average body temperature of the embryos was 13 degrees c with an average hr of 8.7 +/- 0.9 beats per minute ...200919359693
social motivation affects the display of individual discrimination in young and adult japanese quail (coturnix japonica).a simultaneous two-choice test was used to investigate discrimination between a familiar (f) and an unfamiliar conspecific (u) in japanese quail, selected for high (hsr) or low (lsr) social reinstatement behavior (tendency to rejoin and stay close to conspecifics). animals were 1-week unsexed and 6-week-old male quail. one-week-old lsr quail and 6-week-old male hsr quail displayed discrimination after 24 hr of pair contact (p < 0.05), and a tendency to discriminate (p < 0.10) was found in adult ...200919277976
aflatoxin effect on the oocysts morphometry and contribution on the morphology of eimeria bateri bhatia, pandey and pande, 1965 of the japanese quail coturnix japonica in brazil.the purpose of this study was to characterize eimeria bateri oocysts and to evaluate the aflatoxin effect in the morphometry of sporulated oocysts in japanese quails infected naturally. of a total of 50 quails naturally infected by e. bateri were randomly divided into two groups with 25 birds each. in one of them, quails were orally administered with aflatoxin in dose of 0.04 mg/kg body weight previously. both experimental groups shed e. bateri oocysts. these oocysts were subspherical to ellipso ...200919265584
mucuna pruriens seed powder feeding influences reproductive conditions and development in japanese quail coturnix coturnix japonica.this study was designed to test whether mucuna pruriens, a natural source of l-dihydroxyphenylalanine (l-dopa, a dopamine precursor) feeding, can influence development and reproductive conditions in the high food value bird, japanese quail, coturnix coturnix japonica. experiments were performed in both male and female japanese quail. one-week-old quail chicks were divided into three groups of 36 birds each. group i was provided with normal diet and served as control. group ii was provided with f ...200922444229
effects of simulated hypo- and hyper-reproductive conditions on the characteristics of circadian rhythm in hypothalamic concentration of serotonin and dopamine and in plasma levels of thyroxine, triiodothyronine, and testosterone in japanese quail, coturnix coturnix japonica.in this study, hypo- and hyper-reproductive conditions, as measured by concentrations of plasma testosterone in male japanese quail held on long days ld 16:8, were experimentally simulated with injections of 5-hydroxytryptophan (5-htp) and l-dihydroxyphenylalanine, (l-dopa) with 8 h and 12 h phase angle differences between them in intact and melatonin-treated birds. the effects of these treatments were assessed on the characteristics of the circadian rhythm in the hypothalamic concentration of s ...200919142756
[effect of hypoxic argon-containing gas mixtures on developing organism].experimental data on the impact of argon-containing hypoxic breathing mixtures on the early embryonic growth of japanese quail (coturnix coturnix japonica dom.) are presented. the results showed that after 4-day incubation of eggs in an oxygen-nitrogen breathing mixture (13.8-15.0% o2, 86.2-85.0% n2) only 16.7% embryos reached the stage of 3.5-d development. introduction in the hypoxic mixture of argon (55% of the total volume) increased the number of embryos on the stage of 3.5-4 day of normal ...201619140472
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