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[typhoid in chile. permanent susceptibility of salmonella typhi to chloramphenicol and other drugs]. 19863461515
attenuated salmonella becomes versatile vector in multivalent, multipurpose genetic vaccines. 19873477653
an analysis of blood culture isolates from 7 south african teaching hospital centres.the data on blood culture isolates for 1983 and january - july 1984 reported by the antibiotic study group of south africa have been analysed to determine national and regional prevalences of different micro-organisms and resistance to certain antibiotics. although there are significant differences in isolation frequencies between the various centres, overall the five most frequent isolates are staphylococcus aureus (1983 - 15%; 1984 - 14%), escherichia coli (13%), klebsiella spp. (11%; 10%), st ...19863484560
an outbreak of typhoid fever in chandigarh, north india.an outbreak of typhoid fever occurred among 54 hospital nurses after a picnic. the salient features were fever (100%), nausea and vomiting (46%), loose motions and abdominal pain (13%), and palpable splenomegaly (63%). none of the patients had any major complications. blood cultures for salmonella typhi were positive in 81%, blood widal was positive (1:320 or more) in 43% and suggestive (1:160) in 25% of the blood culture positive patients. a comparable number of patients were administered chlor ...19863485847
spurious sulfamethoxazole-trimethoprim resistance of salmonella typhi.several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. we document the phenomenon in salmonella typhi and salmonella paratyphi-a and demonstrate its occurrence in 3 of 17 (18%) lots of mueller-hinton agars now in use in major medical laboratories in lima, peru. the findings are particularly significant because sulfamethoxazole-trimethoprim is an important alternative to chloramphenico ...19863486190
hemophagocytosis accompanying typhoid fever. 19863487778
typhoid colitis with massive lower gastrointestinal bleeding. an unexpected behavior of salmonella typhi.three cases of salmonella colitis with massive lower gastrointestinal hemorrhage are presented. the patients had fever, diarrhea, and abundant hematochezia; blood and bone marrow cultures were positive for salmonella typhi. because of bleeding, all patients underwent hemicolectomy; previous mesenteric arteriograms showed the colonic hemorrhagic sites. the pathologic and radiologic findings are described.19863488182
an international point source outbreak of typhoid fever: a european collaborative investigation.a point source outbreak of salmonella typhi, degraded vi-strain 22, affecting 32 british visitors to kos, greece, in 1983 was attributed by a case-control study to the consumption of a salad at one hotel. this represents the first major outbreak of typhoid fever in which a salad has been identified as the vehicle. the source of the infection was probably a carrier in the hotel staff. the investigation demonstrates the importance of national surveillance, international cooperation, and epidemiolo ...19863488842
iga-driven t cell-mediated anti-bacterial immunity in man after live oral ty 21a vaccine.cellular immunity against salmonella typhi was observed by using a direct anti-bacterial in vitro assay in volunteers orally vaccinated with the live s. typhi mutant strain ty 21a. with this experimental approach, it was demonstrated that ty 21a vaccine also induces cellular immunity against s. paratyphi a and b. interestingly, the mechanism involved in cellular immunity against bacteria seems to be of an antibody-dependent cellular cytotoxicity (adcc) type, with iga acting as the humoral arm an ...19863489034
pharmacological properties of droxicam, a new non-steroidal anti-inflammatory agent.droxicam showed high antiinflammatory activity in carrageenin-induced edema in rat. at doses of 0.25 and 0.5 mg/kg, droxicam was as active as piroxicam and more active than phenylbutazone given at 2.5 and 5 mg/kg. against nystatin-induced edema, droxicam (ed50, p.o., 5, 6, 7, 8 h: 7.5, 12.9, 4.8, 8.4 mg/kg) was 4-11 times more active than phenylbutazone and more than 12 times more active than isoxicam. in cotton pellet-induced granuloma in rats, title compound was as active as suprofen. in u.v. ...19863489870
sex-related differences in fever development of rats.lipopolysaccharide (lps) or partially purified endogenous pyrogen (ep) was injected intravenously into rats of both sexes to induce fever. in lps fever, which was easily produced in 24-h dehydrated rats with an intravenous injection of lps, the female rats showed an attenuated febrile reaction as measured by both the maximum increase of rectal temperature (tre) and the thermal response index (ti). although castrated female rats showed the same magnitude of febrile reaction to that in normal fema ...19873492927
salmonella typhi 205aty, a strain with two attenuating auxotrophic characters, for use in laboratory teaching.the need for p-aminobenzoic acid of a previously reported pab mutant in a salmonella typhi strain causes loss of virulence (mouse median lethal dose by the intraperitoneal route with mucin, ca. 10(7) cfu, versus less than 200 cfu for related pab+ strains). this strain, however, gave p-aminobenzoic acid-independent revertants at a low frequency (ca. 4 x 10(-10) per bacterium per generation). it was therefore given, by transduction and mutation, a transposon-generated, nonreverting (rate, less tha ...19873493975
cellular immunity against salmonella typhi after live oral vaccine. 19873501237
identification of some interleukin-1 (il-1) sensitive organs in vivo using the induction of ornithine decarboxylase (odc) following injection of recombinant il-1 beta in mice.intraperitoneal (i.p.) and intravenous (i.v.) injection of human recombinant interleukin-1 beta (ril-1 beta) in mice produced a 2-4 fold induction of ornithine decarboxylase (odc) in liver and heart six hours after administration. lymphoid organs (thymus and spleen) and brain did not respond to ril-1 beta administration with significant increases in odc. il-1-induced responses in heart seemed not to be secondary to stress induced catecholamine release since the beta-adrenergic antagonist propran ...19873501508
[comparison of ampicillin, chloramphenicol and trimethoprim-sulfamethoxazole (tmp-smz) for the treatment of salmonella typhi infections in clinical practice].ninety-four patients with salmonella typhi infection hospitalized and treatment. widal test results, blood cultures, stool cultures and physical examination findings were admitted infection criteria. ampicillin used 100 mg/kg/day four divided dose in 52 patients, chloramphenicol used 2g/day four divided dose in 28 patients and tmp-smz 160-800 mg used bid in 14 patients. all of the treatments applied for 2 weeks. ampicillin 92.30%, chloramphenicol 82.15% and tmp-smz 85.71% were found success. fev ...19873502016
[the effect of drugs on the immune response].in this study the effects of some drugs on the humoral and cellular immune responses were carried out. on the studying of laboratory animals, the humoral response by the antibody formation against s.typhi and the cellular response by ppd tuberculin of vaccinated animals with bcg, were examined. according to the experimental results, it was observed that trimethoprim/sulfamethoxazole (baktrim) and chloramphenicol did not effect humoral response but inhibited cellular response. it was determined t ...19873502284
serodiagnosis of typhoid fever in paediatric patients by anti-lps elisa.enzyme-linked immunosorbent assay (elisa) and immunoblot analysis showed that up to 50% of the anti-typhoid antibody in sera from blood culture positive paediatric typhoid fever patients is directed against lipopolysaccharide (lps) antigen. anti-salmonella typhi lps elisa was therefore compared to widal agglutination for serodiagnosis of typhoid fever in paediatric patients. sera from 38 paediatric control individuals were elisa negative for anti-s. typhi lps igg; all but 2 of these specimens we ...19873503403
[effect of components of curing mixtures on the antagonistic properties of enterococci and other bacteria isolated from cured meat].effect of sodium chloride, glucose, sodium glutamate, potassium nitrate, sodium nitrate, sodium nitraite, ascorbic acid, sodium citrate, polyphosphates (hamine) on antagonistic activity of enterococci and various bacteria isolated from cured meat against bacillus cereus, bacillus subtilis, salmonella typhi-murium, salmonella cholerae-suis, staphylococcus aureus, clostridium botulinum type a, clostridium botulinum type b, clostridium botulinum type e, clostridium bifermentans, clostridium perfrin ...19873506173
investigation of the response to the enterobacterial common antigen after typhoid vaccination. 19873507980
typhoid fever: successful therapy with cefoperazone.we compared cefoperazone with chloramphenicol in a randomized trial involving the treatment of 25 children with severe typhoid fever. the clinical characteristics of the two treatment groups were comparable on entry into the study. four patients died (three receiving chloramphenicol, one cefoperazone), and an additional two patients received dexamethasone (one per group). excluding these six patients, the response of the cefoperazone group was comparable or superior to that of the chloramphenico ...19863511159
salmonella focal intracranial infections: review of the world literature (1884-1984) and report of an unusual case.focal intracranial infections are unusual manifestations of salmonellosis. forty-three such infections have been reported in the world literature. the clinical data for 34 well-documented cases are reviewed. eleven patients had brain abscess, 19 had subdural empyema, three had epidural abscess, and one had both subdural empyema and brain abscess. brain abscess occurred more often in adults; in contrast, subdural empyema presented more often in children. fever, signs and symptoms of increased int ...19863513285
sensitivity of moore sewer swabs for isolating salmonella typhi.moore swabs (sewer swabs) have been used successfully to culture pathogenic organisms from wastewater. sensitivity seems to depend on the size of the waterway sampled as well as the number of organisms present. in santiago, chile, we placed 24 swabs into the sewers draining the homes of 10 known chronic carriers of typhoid. swabs were positive for salmonella typhi in 5 of the 10 households (50%) and 6 of the 24 swabs placed (25%).19863513705
the use of furoxone: a perspective.acute infectious diarrhoea is a widespread cause of morbidity and mortality. some of the major diarrhoeal diseases are cholera, typhoid fever, shigellosis (bacillary dysentery), salmonellosis, "travellers' diarrhoea", and giardiasis these diseases can be avoided with proper education, sanitation, and hygiene. however, the majority of these diseases occur most frequently in areas of the world where political and social upheaval, poverty, overcrowding, and a lack of education prevail. although vac ...19863514312
[detection of the causative agents of typhoid and paratyphoid fevers in dairy products by the mobile growth method]. 19863514384
[typhoid fever: its diagnosis]. 19863518735
[pathogenesis of the typhoid bacterium carrier state]. 19863520873
[effect of the polysaccharide from klebsiella pneumoniae k8 on the ld50 of salmonella typhi]. 19863526495
the phagocytosis function and the antibody reactivity in the thymectomized and thymectomized-irradiated adult rats.the authors have investigated the influence of the thymus on peritoneal macrophages phagocytosis and antiflagellar agglutinin serum antibodies to "h" salmonella typhi in the adult rat with diminished immunologic potential. the results show that the immune reactions diminish in intensity in the thymectomized animal. the immune reactions are also diminished in the thymectomized animal exposed to radiation as compared to control irradiated but non-thymectomized.19863526526
late intraphagosomal hydrogen ion concentration favors the in vitro antimicrobial capacity of a 37-kilodalton cationic granule protein of human neutrophil granulocytes.we described previously (w.m. shafer, l.e. martin, and j.k. spitznagel, infect. immun. 45:29-35, 1984) the presence of a 37-kilodalton cationic antimicrobial protein (37k cap) in extracts of granules prepared from human polymorphonuclear granulocytes (pmn). in this investigation, we prepared 37k cap from pmn granule extracts by sequential ion-exchange and molecular-sieve chromatography and examined its antimicrobial activity against a number of gram-negative and gram-positive bacteria. at concen ...19863527987
oral immunization of mice with attenuated salmonella enteritidis containing a recombinant plasmid which codes for production of the b subunit of heat-labile escherichia coli enterotoxin.we used salmonella enteritidis serotype dublin strain sl1438, a nonreverting, aromatic-dependent, histidine-requiring mutant, as a recipient for a recombinant plasmid coding for production of the nontoxic b subunit of the heat-labile escherichia coli enterotoxin. the s. enteritidis derivative el23 produced heat-labile enterotoxin subunit b that was indistinguishable from heat-labile enterotoxin subunit b produced by strains of e. coli or salmonella typhi harboring the same plasmid. mice immunize ...19863527989
salmonella meningitis: report of five cases. 19863531003
[growth responses of salmonella typhi in the presence of antibiotics, bile acid and escherichia coli]. 19863531223
[treatment of typhoid fever using ofloxacin. clinical experience and determination of antibiotic diffusion into the mesenteric lymph nodes].nine patients with typhoid fever were given ofloxacin in a daily dosage of 400 mg for ten days. all patients recovered with no relapses. no case of salmonella typhi carriage was recorded. diffusion of ofloxacin into mesenteric lymph nodes was studied in four patients. mean concentration was 1.46 micrograms/g after four 200 mg doses. according to our results, ofloxacin could be considered as one of the alternatives for treating typhoid fever.19863534732
[sensitivity to antibiotics of salmonella typhi and paratyphi strains isolated in algeria 1979-1984].outbreaks of typhoid fever due to multiresistant strains of s. typhi have occurred in some countries. monitoring of the susceptibility of s. typhi and paratyphi strains to antibiotics, especially those used to treat salmonella infections, is therefore essential. in this paper, we report the results of our study of strains isolated in algeria from 1979 through 1984. studied strains were isolated in our laboratory from stool or blood samples or sent to us for confirmation of the diagnosis. we used ...19863534753
norfloxacin in the treatment of bacterial enteric infections.diarrhoeal diseases are a major cause of illness and death among infants and young children, especially in developing countries. they are also the principal cause of illness in tourists who travel to these countries. overall, the main causes of diarrhoea are bacterial. campylobacter, enteropathogenic e. coli, shigella and enterotoxigenic e. coli are, in this order, the most common bacterial enteropathogens isolated in developing countries, and campylobacter and shigella in developed countries. e ...19863535054
[microcalorimetric study of salmonella].we investigated the heat production of 42 different salmonella strains belonging to the serogroups a, b, c, d and e. using columbia- and brain heart infusion broth as growth media, we found 3 different types of heat profiles. salmonella typhi and salmonella choleraesuis showed thermograms which differed in shape from the remaining serotypes. the thermograms of one salmonella dublin isolate which showed an atypical biochemical behaviour could not be put into proper relation to one of these 3 micr ...19863541456
[ultrastructural study of intestinal bacteria grown on endo- and levine-type nutrient media based on nonfood raw materials].the comparative electron-microscopic study of several test strains (salmonella typhi h-901, shigella flexneri la 8516, and escherichia coli 055) grown in experimental endo and levine media prepared on the basis of raw materials unsuitable for human consumption and in commonly used similar media prepared on the basis of sprat hydrolysate has shown the test strains grown in media containing aminopeptide and fodder yeast hydrolysate to retain their typical ultrastructure, which confirms the possibi ...19863541464
the comparative activity of pefloxacin, enoxacin, ciprofloxacin and 13 other antimicrobial agents against enteropathogenic microorganisms.in this study, we compared the activity of pefloxacin, enoxacin and ciprofloxacin against 269 enteropathogenic strains (campylobacter jejuni, enteropathogenic escherichia coli, salmonella typhi, shigella spp., vibrio cholerae and yersinia enterocolitica) with that of rosoxacin, flumequin, nifuroxazide, erythromycin, chloramphenicol, ampicillin, cefotaxime, tetracycline, amikacin, netilmicin, sulfamethoxazole, trimethoprim and co-trimoxazole. pefloxacin, enoxacin and ciprofloxacin were always amo ...19863546145
[detection of microbial variants of salmonella typhi in the bone marrow of typhoid patients and carriers].the immunofluorescence test with the use of antisera to s. typhi and its l-forms permits the detection of the infective agent in bone marrow smears. this diagnostic method is particularly important in cases of carrier state in the latent phase and in differential diagnosis. as revealed in this study, the microbial variants of s. typhi, reacting with antiserum to its l-forms, are present in the bone marrow of carriers. they are localized, as a rule, on the membranes of lymphoid and erythroid cell ...19863548169
evaluation of humoral and cellular immunity in patients with urinary tract infections.the cellular and humoral immunity of 15 patients with recurrent urinary tract infections was assessed during infection. we determined the reactivity of peripheral blood lymphocytes to microbial antigens (ppd, streptolysin o, tetanus toxoid, salmonella typhi and escherichia coli), to allogenic cells, and to mitogens (phytohemagglutinin, concanavalin a, pokeweed mitogen and jacalin). t and b cells were counted by the rosette technique. we also evaluated serum immunoglobulins and c3 and c4 levels b ...19863548856
failure to detect hydrogen-sulphide production in lactose/sucrose-fermenting enterobacteriaceae, using triple sugar iron agar.triple sugar iron agar failed to detect hydrogen sulphide in 44 out of 69 hydrogen-sulphide producing strains of enterobacteriaceae, which at the same time fermented lactose and/or sucrose. the species involved were salmonella typhi, salmonella enteritidis, citrobacter freundii, escherichia coli, and proteus vulgaris. by contrast, no false-negative reactions were observed in 74 strains, which fermented neither lactose nor sucrose. failure to detect hydrogen sulphide was probably due to acidifica ...19873551500
cytopathogenic effect of salmonella typhi gifu 10007 on m cells of murine ileal peyer's patches in ligated ileal loops: an ultrastructural study.an electron microscopic study revealed that, within 30 min after inoculation into the ligated ileal loop of anesthetized mice, cells of salmonella typhi gifu 10007 adhered to the m cell surface of peyer's patch lymphoid follicle epithelium, and induced almost complete destruction of m cells. the m cell cytoplasms were pinched off and extruded from the epithelial lining into the luminal space together with the lymphoid cells primarily enfolded into the corresponding m cells. when two or more m ce ...19863553868
[toxic megacolon caused by typhoid fever]. 19873563067
acute autonomic neuropathy with salmonella typhi infection. a case report.acute autonomic neuropathy is a well-described clinical entity that is easy to diagnose with appropriate bedside tests. this condition is described in a patient who had a concurrent salmonella typhi infection. the relationship between the two conditions is not entirely clear, but the simultaneous disappearance of the s. typhi from stool, the drop in widal titres and rather early recovery of the autonomic neuropathy tend to implicate s. typhi as the causative agent.19873563822
[mitogenic action of bacterial t-independent antigens under various conditions of lymphocyte culture].the mitogenic response of lymphocytes in mouse spleen cell culture to the action of salmonella typhi lipopolysaccharide (lps) and vi-antigen under different experimental conditions has been studied. the density of the culture has been shown to influence the activation of lymphocytes with vi-antigen. thus, macrophages stimulate mitogenesis at the concentration of lymphocytes equal to 1.0-1.2 x 10(6) cells/ml and suppress it when this concentration increases tenfold. the method used for the purifi ...19873564784
[sensitivity to chlorine-containing disinfectants of salmonella typhi strains with transmissible drug resistance].sensitivity of 201 s. typhi strains to antibiotics and chlorine containing disinfectants was studied. the strains were isolated in the regions of the left bank ukraine. among the typhoid cultures the proportion of r+ strains was on the whole equal to 29.3 per cent. chloramine resistant strains amounted to 21.8 per cent. among chloramine resistant strains the number of the cultures carrying r plasmids was 3 times higher than that of the strains sensitive to disinfectant. simultaneous transfer of ...19873566232
role of splenic stroma in the action of bacterial lipopolysaccharides on radiation mortality: a study in mice carrying the slj allele.slj/+ mice display a slight macrocytic anaemia due to a defect in their haemopoietic organ stroma. they have a deficient endogenous spleen colony (cfu-end) formation following sublethal doses of gamma-radiation compared with their normal +/+ littermates, which is likely to be due to the low pre-irradiation cfu-s content of the slj/+ spleen. cfu-s in these congenic mice do not differ in their sensitivity to gamma-irradiation or stem cell-activating factor. while injection of +/+ mice with 10 micr ...19873568089
production of vi monoclonal antibodies and their application as diagnostic reagents.serum antibodies to vi antigen were detected in mice immunized with the purified antigen but not with vi-bearing salmonella typhi whole cells. fusion of the spleen cells from one of the vi antibody-producing mice with nsi myeloma cells produced four stable hybridomas that secreted antibodies to vi. monoclonal antibodies from these four clones were all of the immunoglobulin g class and, as determined by competition, appeared to have the same epitope specificity. despite their immunoglobulin g nat ...19873571457
invasiveness of salmonella typhi strains in hela s3 monolayer cells.the internalization and intracellular multiplication, i.e., the invasiveness, of salmonella typhi strains recently isolated from typhoid fever patients were confirmed in hela cell monolayers. when stained with giemsa solution, intracellular bacteria were 0.6 x 1.2 micron in size and stained purple, whereas extracellular bacteria associated or not with the hela cell surface were 1.0 x 3.0 micron and stained deep blue. strain gifu 10007 was internalized into 23% of the hela cells within 10 min aft ...19863574155
diagnosing enteric fever: a reappraisal.the diagnostic value of 19 clinical and laboratory events was assessed in 150 enteric fever cases. a logistic regression analysis revealed only 8 predicitive variables for the presence or absence of enteric fever. these were, in order of importance, the white blood cell count, the s. typhi h agglutinin titre, body temperature, the s. typhi o agglutinin titre, the s. paratyphi a h agglutinin titre, the s. paratyphi b h agglutinin titre, age and the fever pattern. this study showed that objective ...19863576287
the enhancing effect of exercise on the production of antibody to salmonella typhi in mice.the effect of long-term regular and moderate exercise on antibody production has been studied in mice. healthy mice were trained to run for 10 min twice a day, and antibody levels following immunization were compared with those of healthy sedentary control mice. the antibody levels of the running mice were significantly higher than those of the sedentary animals at all times tested, from 1 to 13 wk post immunization. overall, the antibody titres of the runners were 2.76 times that of the control ...19873583317
gas chromatographic detection of bacteria causing enteric fever. 19873584377
does chloramphenicol remain the drug of choice for typhoid?of 2356 strains of salmonella typhi isolated in britain in the 8-year period 1978-85, 2345 (99.53%) were sensitive to all antibiotics tested and 11 (0.47%) were chloramphenicol-resistant; chloramphenicol resistance was plasmid-mediated in 6 strains. it is concluded that chloramphenicol remains a satisfactory first-line choice of drug for typhoid fever in britain.19873595753
enterobacteria and ankylosing spondylitis. 19873602954
morbidity and mortality in a diarrhoeal diseases hospital in bangladesh.records of all patients who were admitted to or who died in dhaka hospital of the international centre for diarrhoeal disease research, bangladesh (icddr,b) between july 1980 and 30th june 1981 were reviewed to identify epidemiological characters associated with in-hospital diarrhoeal diseases-related deaths. information on aetiological agents, age, sex, major complications, nutritional status and level of dehydration were analysed. over the one-year period, 3251 patients were admitted to the me ...19863603613
[dependence of the intensity of the immune response on the characteristics of the formation of a salmonella typhi antigen depot on erythrocytes].experiments on inbred mice, opposite in the capacity of their erythrocytes for the sorption of s. typhi antigens, have revealed that the intensity of immune response to the antigens depends on the functional potency of immunocompetent cells and the conditions of their priming. the optimal manifestations of the conditions for lymphocyte priming depends on the character of the antigen fixation by erythrocytes and antigen supply from the depot into the blood stream.19873604510
[pathogenetic and immunological characteristics of different forms of the salmonella typhi carrier state].infectious granulomas with macrophages containing salmonella typhi have been detected in the immune organs of the intestine of typhoid patients by means of morphological investigation techniques, immunofluorescent and electron microscopy. this suggests that typhoid granulomas form the basis of s. typhi primary carriership complicated by the relapses of this infection in cases of weakened cell-mediated immunity, which is proved by a decrease in the level of t-lymphocytes and by increased leukocyt ...19873604512
[comparative serology in typhoid fever in children]. 19873606784
detection of group d salmonellae in blood culture broth and of soluble antigen by tube agglutination using an o-9 monoclonal antibody latex conjugate.latex particles sensitized with a salmonella o-9 monoclonal antibody were used to detect group d salmonellae in blood culture broths. the system was found to be 100% accurate when used on 104 clinical specimens that included 13 s. typhi isolates. enteric fever could thus be diagnosed as early as 1 day after the initiation of culture in some (18%) cases. the latex particles were also used in an experimental situation to detect soluble s. typhi lipopolysaccharide by a tube agglutination method. th ...19873611308
sulfonamide and trimethoprim resistance in salmonella typhi. 19873611328
genetic characteristics of the salmonella typhi strain ty21a vaccine. 19873619995
systemic and intestinal immunities after different typhoid vaccinations.the comparative studies of systemic and intestinal immunities to s. typhi were performed in 29 healthy volunteers during 2 years after receiving oral vaccination with attenuated s. typhi ty21a in gelatin capsule, parenteral vaccination with acetone inactivated or heat inactivated-phenol preserved s. typhi ty2. the methods used were immunobead elisa for total secretory iga and indirect elisa for specific secretory iga in the intestinal lavage fluid. the specific systemic igg, igm and anti-o, anti ...19873620049
responses of baboons to traditionally pyrogenic agents.it is not clear whether baboons develop fever in response to endotoxin or other pyrogens. we injected various pyrogens intravenously in 12 unrestrained baboons (papio ursinus) and measured their body temperature using intra-abdominal radiotelemeters. serum iron concentration was also measured. the baboons developed fever after injection of killed staphylococcus aureus (5 x 10(7) organisms/kg). no significant fever was measured after injection of lipopolysaccharide (salmonella typhosa) (0.1, 8, 4 ...19873621085
the effect of some thymic extracts on antibody formation in rats and mice.the influence of some thymic extracts tp1 and tp2 on the number of hemolytic plaque-forming cells (pfc) in the mouse spleen, on the titre of serum antibodies, s-typhy antiflagelar agglutinins and anti-influenza antibodies, was investigated. the results have shown that the hemolytic plaque-forming cells in the mouse spleen increase significantly, 2.3 times under the influence of the tp2 thymic extract in rap mice inoculated with sheep red cells. in wistar rats, inoculated with a formol-inactivate ...19863643647
chloramphenicol-resistant salmonella typhi in durban, south africa.a retrospective survey was undertaken to determine the incidence of chloramphenicol-resistant salmonella typhi in durban, south africa. of the 3313 strains tested over a 7 year period 20 (0.6%) showed intermediate resistance to chloramphenicol and 19 (0.6%) were fully resistant. resistance to chloramphenicol was found to fluctuate over the period studied (0-2%). resistance to ampicillin/amoxycillin was encountered less frequently (0-0.9%). for the present we would still recommend chloramphenicol ...19873649097
bacteremia caused by a lactose-fermenting, multiply resistant salmonella typhi strain in a patient recovering from typhoid fever.a female patient suffered a typical attack of typhoid fever due to a lactose-negative, fully susceptible salmonella typhi strain. during convalescence she became febrile, and a lactose-fermenting s. typhi strain resistant to ampicillin, chloramphenicol, tetracycline, and trimethoprim was isolated from blood culture. this isolated was shown to harbor a plasmid which cotransferred lactose fermentation and antibiotic resistance.19873650270
prevention of typhoid fever in nepal with the vi capsular polysaccharide of salmonella typhi. a preliminary report.we conducted a pilot study followed by a large clinical trial in nepal of the use of the capsular polysaccharide of salmonella typhi (vi) as a vaccine to prevent typhoid fever. in the pilot study, involving 274 nepalese, there were no significant side effects of the vi vaccine; about 75 percent responded with a rise in serum antibodies of fourfold or more. in the clinical trial, residents of five villages were given intramuscular injections of either vi or, as a control, pneumococcus vaccine dis ...19873657877
multiple drug resistant salmonella typhi in bangladesh. 19863668196
[diarrhea and acquired immunodeficiency syndrome in the tropics (tropical aids). the place of digestive endoscopic examinations in diagnosing opportunistic infections].a.i.d.s. has revealed some parasitic, microbiotic, mycotic or viral diseases causing diarrhea or has given a revival of interest to them. so are cryptosporidium, isospora belli, salmonella typhi murium, cytomegalovirus. some easy techniques lead to the diagnosis of some pathogenic agents, such as parasitologic diagnosis of cryptosporidium. some other pathogenic agents call for a biopsy in view of an histopathology test: it is the case of cytomegalovirus of which the diagnosis is stated positivel ...19873670029
successful treatment of typhoid fever in children with parenteral ceftriaxone.eight children, seriously ill with bacteriologically proven salmonella typhi septicemia, were successfully treated with a single daily intramuscular injection of 2 g ceftriaxone given for 5-7 days. all children improved clinically within 48 h of starting therapy and all except 1 became afebrile within 5 days. none relapsed during the 4-week follow-up study.19873672051
platelet-activating factor: an endogenous mediator for bowel necrosis in endotoxemia.we have developed a model of isochemic bowel necrosis in the rat by injecting platelet-activating factor (paf) or paf in combination with bacterial endotoxin. paf causes profound hypotension, and it has been suggested that it is released during endotoxin shock. because ischemic bowel necrosis is often associated with shock or infection, it is possible that paf is the endogenous mediator that causes shock and bowel necrosis during sepsis. in this study, we have demonstrated that: 1) normal intest ...19873678700
serodiagnosis of typhoid fever. 19873679455
counter-immunoelectrophoresis as a diagnostic test for typhoid fever. 19873679456
outbreak of chloramphenicol resistant typhoid fever. 19873679457
salmonella typhi meningitis. 19873679461
characteristics of humoral and cellular immunity to salmonella typhi in residents of typhoid-endemic and typhoid-free regions. 19873680988
vi capsular polysaccharide-protein conjugates for prevention of typhoid fever. preparation, characterization, and immunogenicity in laboratory animals.the vi has proven to be a protective antigen in two double masked, controlled clinical trials in areas with high rates of typhoid fever (approximately 1% per annum). in both studies the protective efficacy of the vi was approximately 70%. approximately 75% of subjects in these areas responded with a fourfold or greater rise of serum vi antibodies. in contrast, the vi elicited a fourfold or greater rise in 95-100% of young adults in france and the united states. methods were devised, therefore, t ...19873681191
antibacterial activity of lymphocytes armed with iga. 19873687537
presence of endotoxemia and its relationship to liver dysfunction in patients with typhoid fever.twenty-one patients with typhoid fever were studied to evaluate the presence of endotoxin in peripheral blood and its relationship to the incidence and features of hepatic dysfunction which may occur during this disease. the limulus test for endotoxin was positive in the plasma samples of all patients prior to treatment. liver dysfunction, as assessed by fasting and postprandial serum bile acid levels and by standard biochemical tests, occurred in 90% of patients. in seven, the injury was purely ...19873692608
[absence of the antigen h:z66 in 2355 strains of salmonella typhi from madagascar and several countries of tropical africa].the presence of the new flagellar antigen h:z66 (guinée, 1981) was investigated among 2,355 strains of s. typhi isolated from 1981 to 1985 in madagascar and some countries of tropical africa: burundi, ivory coast, gabon, mauritania, central african republic, rwanda, senegal, zaïre. a method based on the immobilization of motile strains in soft agar with immunserum anti-h:d, was used to detect strains carrying antigen h:z66. no african and malagazy strains had antigen h:z66, irrespective to their ...19863698151
specific immunoglobulin-secreting human blood cells after peroral vaccination against salmonella typhi.studies on intestinal immunity in animals have suggested that lymphocytes, after sensitization in peyer's patches and maturation in regional lymph nodes, home via the circulation to the intestinal wall, where they secrete antibodies into the lumen. to detect such homing cells in the blood of human volunteers given oral, attenuated salmonella typhi vaccine, we used the solid-phase enzyme-linked immunospot assay for s. typhi-specific antibody-secreting cells (ascs). ascs were found in eight of 10 ...19863701119
lipid peroxidation by gamma-radiation in salmonella typhi membranes: the effect of ascorbic acid.lipid peroxidation in isolated membranes of salmonella typhi occurred following gamma irradiation. the presence of the sensitizer, ascorbic acid, during irradiation resulted in a stimulation in the level of peroxidation. this enhancement of lipid damage could be prevented by the addition of the free radical scavenger sodium formate. catalase and superoxide dismutase appeared less effective in this respect. peroxidation in an unsaturated fatty acid and a phospholipid was also affected by gamma ra ...19863718525
prevention of endotoxin-induced gastrointestinal damage by cv-3988, an antagonist of platelet-activating factor. 19863720841
salmonella hepatitis: detection of salmonella antigens in the liver of patients with typhoid fever. 19863722872
stellate maculopathy due to salmonella typhi. a case report.a case of typhoid fever complicated by bilateral chorioretinitis and monolateral stellate maculopathy is reported. clinical findings are discussed.19863725313
summary of an international workshop on typhoid fever. 19863726393
ureteral blockade sensitizes to the generalized shwartzman reaction.renal cortical necrosis (rcn) has been reported in the normal kidney of patients with a contralateral ureteral occlusion (uo). so far, studies have examined the mechanisms protecting the affected kidney from glomerular thrombosis and cortical necrosis; but to the authors' knowledge, none has ever investigated the potential role of uo on the occurrence of the associated disseminated intravascular coagulation (dic) episode leading to rcn. female rats with a ligature of the right or left ureter wer ...19863740212
unusual complications of typhoid fever. 19863742582
foot abscess due to salmonella typhi: a case report.a case of foot abscess due to salmonella typhi is reported in a 37-year-old yemeni man. the abscess was surgically drained and the wound healed without antibiotic therapy.19863750402
potential enteropathogens in fish from lake kivu. 19863753053
complexing of bacterial lipopolysaccharide with lung surfactant.lipopolysaccharides (lps) from escherichia coli, salmonella typhi, klebsiella pneumoniae, serratia marcescens, or pseudomonas aeruginosa were mixed with pulmonary surfactant to investigate their in vitro interaction. after 6 h of incubation at 37 degrees c, lps-surfactant mixtures were examined by sucrose density gradient centrifugation. the e. coli lps-surfactant mixture was examined by immunoelectron microscopy with protein a-colloidal gold. the binding that occurred between lps and the surfac ...19863754848
[aspects of typhoid fever in children].some aspects of typhoid fever in 77 children are discussed. there were 48 boys and 29 girls and their ages ranged from 1 month to 12 years. the patients were treated with chloramphenicol 100 mg/kg/d during the first 2 weeks and with either amoxycillin (100 mg/kg/d) or ampicillin (200 mg/kg/d) during the third week. the average duration of fever was 5.2 days. there was 1 relapse and 1 child, a baby aged 1 month, died. the correct diagnosis was not suspected by the referring doctor in 38% of the p ...19863764609
novobiocin-brilliant green-glucose agar: new medium for isolation of salmonellae.a new medium, called novobiocin-brilliant green-glucose (nbg) agar, was developed for the isolation of salmonella spp. and evaluated against other conventionally used media including bismuth sulfite, xylose-lysine decarboxylase, brilliant green-sulfa, hektoen enteric, and salmonella-shigella agars. nbg had recovery rates comparable to the other enteric media tested with pure cultures as well as with naturally contaminated amphibian and reptile waters and fecal specimens. however, nbg, hektoen en ...19863767359
imported typhoid fever. 19863772323
counter immunoelectrophoresis (ciep) for serological diagnosis of typhoid fever. 19863781590
bone marrow culture in the diagnosis of enteric fever. 19863781607
comparison of counter current immunoelectrophoresis and widal tests in the diagnosis of typhoid fever in childhood. 19863781608
some gale mutants of salmonella choleraesuis retain virulence.gale mutants were isolated from three mouse-virulent strains of salmonella choleraesuis (of group c1, o antigen 6,7) by selection for resistance to 2-deoxygalactose. the gale derivative of strain 381 comprised two components: galactose sensitive, thought to be the original mutant; and galactose resistant, presumably by a second mutation reducing galk or galt function or both. the galactose-sensitive component had an intraperitoneal 50% lethal dose for balb/c mice of ca. 4 x 10(6) cfu, whereas th ...19863781619
the comparative effectiveness, under epidemic conditions, of the intradermal versus subcutaneous antityphoid vaccination estimated by epidemiological surveillance and laboratory tests.the inhabitants of a territory in which a severe epidemic of typhoid fever appeared, were immunized intradermally or subcutaneously with heat-inactivated phenol preserved typhoid vaccine. the great majority of individuals received booster doses during the following three years. the specific immunization together with other epidemiologic and hygienic measures resulted in a quick extinction of the epidemic and the reduction in time of the number of typhoid carriers; no case of typhoid fever occurr ...19863788347
passive hemagglutination test for enteric fever.a passive hemagglutination (pha) test for serodiagnosis of enteric fever was developed by sensitizing glutaraldehyde-preserved erythrocytes with lipopolysaccharide from salmonella serogroups a, b, c, and d singly or simultaneously. the lipopolysaccharide-sensitized erythrocytes were tested with sera from 200 blood donors, 100 patients whose hemoculture was positive for salmonella species, and 10 patients septicemic for other members of the family enterobacteriaceae. the pha test was positive in ...19873793865
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