Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| phosphatidylcholine-specific phospholipase c from achromobacter xylosoxidans. | a new phosphatidylcholine-specific phospholipase c (ec 3.1.4.3.) has been isolated from the culture broth of achromobacter xylosoxidans. growth conditions were optimized for maximum production of the enzyme. a temperature-sensitive synthesis was established. chromatography on cm sephadex and polyacrylamide gel electrophoresis of the native enzyme revealed a number of isozymes. the water soluble substrate for phospholipase p-nitrophenylphosphorylcholine was not hydrolysed by the enzyme. | 1993 | 8511996 |
| purification and characterization of novel n-acyl-d-aspartate amidohydrolase from alcaligenes xylosoxydans subsp. xylosoxydans a-6. | alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) produced n-acyl-d-aspartate amidohydrolase (d-aaase) in the presence of n-acetyl-d-aspartate as an inducer. the enzyme was purified to homogeneity. the enzyme had a molecular mass of 56 kda and was shown by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page) to be a monomer. the isoelectric point was 4.8. the enzyme had maximal activity at ph 7.5 to 8.0 and 50 degrees c, and was stable at ph 8.0 and up to 45 de ... | 1993 | 7763985 |
| production, purification, and characterization of d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6. | the best inducers for d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) were a poor substrate, n-acetyl-gamma-methyl-d-leucine, and an inhibitor, n-acetyl-d-alloisoleucine. the enzyme has been homogeneously purified. the molecular weight of the native enzyme was estimated to be 58,000 by gel filtration. a subunit molecular weight of 52,000 was measured by sds-page, indicating that the native protein is a monomer. the isoelectric point was 5.2. the enzyme was ... | 1993 | 7763986 |
| purification and characterization of the dissimilatory nitrite reductase from alcaligenes xylosoxidans subsp. xylosoxidans (n.c.i.m.b. 11015): evidence for the presence of both type 1 and type 2 copper centres. | dissimilatory nitrite reductase was isolated from extracts of alcaligenes xylosoxidans subsp. xylosoxidans (n.c.i.m.b. 11015), after activation of crude extracts by the addition of copper(ii) sulphate. the enzyme was purified by a combination of (nh4)2so4 fractionation and cationic-exchange chromatography to 93% homogeneity as judged by sds/page. sds/page and spray m.s. showed that the enzyme had a subunit m(r) of 36.5 kda. the copper content was 3.5 +/- 0.8 cu atoms/trimer of m(r) 109,500. e.p. ... | 1993 | 8240262 |
| achromobacter xylosoxidans osteomyelitis. | 1993 | 8280199 | |
| catheter-associated sepsis due to alcaligenes xylosoxidans in a child with aids. | 1993 | 8357382 | |
| achromobacter xylosoxidans keratitis following penetrating keratoplasty. | achromobacter xylosoxidans is a bacterium not previously reported in a corneal infection. we present a case of infectious keratitis caused by this organism, occurring 1 month following penetrating keratoplasty. | 1993 | 8481378 |
| achromobacter xylosoxidans (alcaligenes xylosoxidans subspecies xylosoxidans) bacteremia after liver transplantation. | 1993 | 8294634 | |
| x-ray scattering using synchrotron radiation shows nitrite reductase from achromobacter xylosoxidans to be a trimer in solution. | we demonstrate here the applicability of x-ray scattering for studying molecular conformation of multimeric proteins in solution by using synchrotron radiation to extend the range of data collection to include medium angles (ca. 3-4 degrees). we have been able to define the solution structure of the dissimilatory nitrite reductase of achromobacter xylosoxidans (axnir), an enzyme for which there are conflicting reports as to the nature of its multimeric structure. quantitative interpretation of t ... | 1993 | 8338833 |
| 4-methylumbelliferyl-beta-n-acetylglucosaminide hydrolysis by a high-affinity enzyme, a putative marker of protozoan bacterivory. | hydrolysis of an artificial fluorogenic substrate, 4-methylumbelliferyl-beta-n-acetylglucosaminide, has been studied in a monoculture predator-prey system with either a flagellate (bodo saltans) or a ciliate (cyclidium sp.) fed upon pure bacterial culture (aeromonas hydrophila or alcaligenes xylosoxidans). aeromonas hydrophila produced a low-affinity beta-n-acetylglucosaminidase-like enzyme (k(m), >>100 mumol liter) but alcaligenes xylosoxidans did not. inoculation of both bacterial strains with ... | 1993 | 16349049 |
| structure of azurin from achromobacter xylosoxidans ncib11015 at 2.5 a resolution. | the crystal structure of azurin from a denitrifying bacterium, achromobacter xylosoxidans ncib11015, has been refined at 2.5 a resolution using diffraction data obtained by means of synchrotron radiation at kek. crystals suitable for x-ray experiment were obtained by the macro-seeding method and an intensity data were obtained on imaging plates mounted on a weissenberg camera (rmerge = 0.09). the initial model was obtained by the molecular replacement method using the structure of azurin from al ... | 1994 | 7706206 |
| investigation of hospital-acquired infections due to alcaligenes denitrificans subsp. xylosoxydans by dna restriction fragment length polymorphism. | we demonstrate that dna restriction fragment length polymorphism determined by pulsed-field gel electrophoresis is very useful in the investigation of the epidemiology of hospital-acquired infections caused by alcaligenes denitrificans subsp. xylosoxydans. this approach showed that hospital-acquired infections caused by this opportunistic pathogen over a 6-month period in 10 patients hospitalized in an intensive care unit and a surgical unit were not a true outbreak. in addition, this molecular ... | 1994 | 7913093 |
| epr and electron nuclear double resonance (endor) studies show nitrite binding to the type 2 copper centers of the dissimilatory nitrite reductase of alcaligenes xylosoxidans (ncimb 11015). | epr and 1h, 14,15n endor spectra are described for the type 1 and type 2 cu(ii) centers of dissimilatory nitrite reductase (nir) from alcaligenes xylosoxidans. the study was carried out on preparations of nir containing both type 1 and type 2 cu sites, and also on preparations of lower activity which contained essentially only type 1 cu centers. this has enabled endor studies of type 1 and type 2 sites to be carried out largely independently of each other, by appropriate choice of the excitation ... | 1994 | 8136351 |
| proper combination for alcaligenes xylosoxidans subsp. xylosoxidans. | 1994 | 7814519 | |
| evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates. | the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ... | 1994 | 7933531 |
| [susceptibilities of glucose non-fermentative gram-negative bacilli to antibiotics]. | glucose non-fermentative gram-negative bacilli are important nosocomial pathogens. this study concerned with susceptibilities to antibacterial agents of strains of glucose non-fermentative gram-negative bacilli that were isolated from cultures of clinical materials at 123 hospital laboratories throughout japan from september to december of 1991. the tests for susceptibilities were performed according to the disk dilution method recommended by nccls. the following bacteria were tested: pseudomona ... | 1994 | 7933532 |
| combined nickel-cobalt-cadmium resistance encoded by the ncc locus of alcaligenes xylosoxidans 31a. | the nickel-cobalt-cadmium resistance genes carried by plasmid ptom9 of alcaligenes xylosoxidans 31a are located on a 14.5-kb bamhi fragment. by random tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. the ncc locus causes a high-level combined nickel, cobalt, and cadmium resistance in strain ae104, which is a cured derivative of the metal-resistant bacterium alcaligenes eutrophus ch34. ncc is not expressed in escherichia coli. the nre ... | 1994 | 7961470 |
| controlled clinical evaluation of isolator and esp aerobic blood culture systems for detection of bloodstream infections. | a controlled clinical evaluation comparing the isolator system (wampole laboratories, cranbury, n.j.) and the esp 80a blood culture bottle in the automated esp system (difco laboratories, detroit, mich.) was performed with 10,535 blood culture sets from patients with suspected septicemia. of 1,150 positive cultures, 844 positive cultures from 285 patients with 394 septic episodes fulfilled the study criteria for minimum blood sample requirements in each system and clinical significance of isolat ... | 1994 | 8077401 |
| metal-characterization of n-acyl-d-glutamate amidohydrolase from pseudomonas sp. strain 5f-1. | n-acyl-d-glutamate amidohydrolase (d-agase) from pseudomonas sp. 5f-1 was a zinc-metalloenzyme which contained 2.06-2.61 g.atom of zn per mole of enzyme. the zinc atom was required for the catalytic activity and stability of the enzyme. the n-terminal amino acid sequence of pseudomonas sp. 5f-1 d-agase showed 32% identity to that of alcaligenes xylosoxydans subsp. xylosoxydans a-6. | 1995 | 7549100 |
| nosocomial infection by alcaligenes xylosoxidans in neutropenic patients. | 1995 | 7567842 | |
| identification of a carbenicillin-hydrolyzing beta-lactamase in alcaligenes denitrificans subsp. xylosoxydans. | eleven strains of alcaligenes denitrificans subsp. xylosoxydans produced a beta-lactamase with a pi of 5.7 with kinetic data characteristic of a pse-1-type enzyme. a carb-type enzyme was identified by using an intragenic dna probe of blacarb. hybridization of genomic dna after xbai restriction and pulsed-field electrophoresis suggested a chromosomal location for the gene. | 1995 | 7793891 |
| meningitis caused by alcaligenes (achromobacter) xylosoxidans associated with epidural catheter. | 1995 | 8655217 | |
| degradation of pcb in different soils by inoculated alcaligenes xylosoxidans. | the degradation of pcb in soils by the biphenyl-utilising strain alcaligenes xylosoxidans was studied in different soil types. in addition to the congener specificity, significant differences in the degradation of pcb by the strain in the different soil types were observed. efficiency of degradation was generally better in sterilised soils, but the differences were not as significant as the differences observed between different soil types. these results indicate that the degradation of pcb is p ... | 1995 | 8578309 |
| evidence for two distinct azurins in alcaligenes xylosoxidans (ncimb 11015): potential electron donors to nitrite reductase. | we have isolated two type 1 copper-containing proteins (m(r) approximately 13k) from alcaligenes xylosoxidans (ncimb 11015) grown under denitrifying conditions. amino acid sequence analysis of these two proteins shows one to be the previously identified azurin (ambler, 1971), which we shall call azurin i, and the other to be a related, but previously undescribed, blue copper protein which we show to also be an azurin and propose to call azurin ii. thus, ncimb 11015 becomes the second system wher ... | 1995 | 7640272 |
| epidemiological investigation of infections due to alcaligenes species in children and patients with cystic fibrosis: use of repetitive-element-sequence polymerase chain reaction. | twenty-one isolates of alcaligenes species were recovered from the respiratory tract of 16 patients at texas children's hospital over a 1-year period. all but one were identified as alcaligenes xylosoxidans; the remaining isolate was identified as alcaligenes faecalis (formerly alcaligenes odorans). thirteen of 21 isolates were from the sputum of eight patients with cystic fibrosis (cf), two of whom were persistently colonized. the remaining isolates were recovered from intubated children. patte ... | 1995 | 7795082 |
| the substrate-binding site in cu nitrite reductase and its similarity to zn carbonic anhydrase. | here we investigate the structure of the two types of copper site in nitrite reductase from alcaligenes xylosoxidans, the molecular organisation of the enzyme when the type-2 copper is absent, and its mode of substrate binding. x-ray absorption studies provide evidence for a fourth ligand at the type-2 cu, that substrate binds to this site and indicates that this binding does not change the type-1 cu centre. the substrate replaces a putative water ligand and is accommodated by a lengthening of t ... | 1995 | 7796265 |
| cloning and sequencing of a gene encoding d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6 and expression of the gene in escherichia coli. | the gene encoding the d-aminoacylase of alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) was cloned and its complete nucleotide sequence was identified. the d-aminoacylase structural gene consists of 1452 nucleotides and encodes 484 amino acid residues. the molecular weight of d-aminoacylase was calculated to be 51,918. this value agreed well with the apparent molecular weight of 52,000 found for the purified enzyme from alcaligenes a-6 by sodium dodecyl sulfate (sds)-polyacryl ... | 1995 | 8541651 |
| primary structure of n-acyl-d-glutamate amidohydrolase from alcaligenes xylosoxydans subsp. xylosoxydans a-6. | the gene coding the n-acyl-d-glutamate amidohydrolase of alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) was cloned and its complete dna sequence was determined. the n-acyl-d-glutamate amidohydrolase structural gene consists of 1,464 nucleotides and encodes 488 amino acid residues. the molecular weight of the enzyme was calculated to be 51,490. this value is close to the apparent molecular weight of 59,000 determined for the purified enzyme from alcaligenes a-6 by sodium dodec ... | 1995 | 8537313 |
| purification and characterization of n-carbamoyl-l-amino acid amidohydrolase with broad substrate specificity from alcaligenes xylosoxidans. | n-carbamoyl-l-amino acid amidohydrolase was purified to homogeneity for the first time from alcaligenes xylosoxidans. the enzyme showed high affinity toward n-carbamoyl-l-amino acids with long-chain aliphatic or aromatic substituents, and hydrolyzed those with short-chain substituents quite well. the enzyme hydrolyzed n-formyl- and n-acetylamino acids quickly and very slowly, respectively. the enzyme did not hydrolyze beta-ureidopropionate and ureidosuccinate. the relative molecular mass of the ... | 1995 | 8590654 |
| characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated soil. | in this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ... | 1995 | 7641143 |
| [frequency of clinical isolation of glucose non-fermentative gram-negative rods and their susceptibilities to antibacterial agents]. | a comparison was made for frequencies of isolation o glucose non-fermentative gram-negative rods ((g)nf-gnr) from clinical specimens during a period from july, 1986 to june, 1987 (the first period) and that from january, 1994 to december, 1994 (the second period). also, minimum inhibitory concentrations of principal drugs were determined against these isolates. the obtained results are summarized as follows: 1. thirty four (34) species of (g)nf-gnr were found from 35,200 clinical specimens in th ... | 1995 | 7474336 |
| antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens). | the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ... | 1995 | 7587049 |
| [comparative susceptibility of ochrobactrum anthropi, agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes denitrificans subsp. denitrificans, alcaligenes denitrificans subsp. xylosidans and bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams]. | ochrobactrum anthropi, formerly known as "achromobacter sp." or cdc group vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). o. anthropi is a gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. the susceptibility of 13 strains of o. anthropi was determined by agar diffusion method and compared to those of type strains of agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes ... | 1995 | 7567111 |
| alcaligenes xylosoxidans-associated infection in an infant with cholesteatoma. | 1995 | 7567305 | |
| unsuccessful treatment of capd peritonitis caused by alcaligenes xylosoxidans subsp. denitrificans. | 1995 | 8570874 | |
| nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems. | dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ... | 1995 | 16535048 |
| structure of a new azurin from the denitrifying bacterium alcaligenes xylosoxidans at high resolution. | it has been reported previously that alcaligenes xylosoxidans (nc1mb 11015) grown under denitrifying conditions produces two azurins instead of the single previously identified azurin [dodd, hasnain, hunter, abraham, debenham, kanzler, eldridge, eady, ambler & smith (1995). biochemistry. in the press]. the new azurin, called azurin ii, has been crystallized as blue elongated rectangular prisms with the tetragonal space group p4(1)22 and unit-cell parameters a = b = 52.65, c = 100.63 a. x-ray cry ... | 1995 | 15299774 |
| direct-method structure determination of the native azurin ii protein using one-wavelength anomalous scattering data. | the one-wavelength anomalous scattering (oas) x-ray diffraction data of azurin ii, a copper-containing protein from alcaligenes xylosoxidans were collected at the photon factory, japan at a 'routine' wavelength of 0.97 a. the structure had been originally solved by the molecular-replacement method [dodd, hasnain, abraham, eady & smith (1995). acta cryst. d51, 1052-1064]. as a technique of ab initio structure determination, the direct method [fan, hao, gu, qian, zheng & ke (1990). acta cryst. a46 ... | 1996 | 15299601 |
| structure solution of azurin ii from alcaligenes xylosoxidans using the laue method: possibility of studying in situ redox changes using x-rays. | we have recently demonstrated that x-rays can be used for changing the redox states of the metal centre in metalloproteins [murphy et al. (1995). j. synchrotron rad. 2, 64-69]. the possibility of using the laue method for studying the structural changes associated with such x-ray-induced reactions is explored by applying the method to the structure determination of a new azurin (hereafter referred to as azurin ii) from the denitrifying bacterium alcaligenes xylosoxidans. laue x-ray diffraction d ... | 1996 | 16702653 |
| reactor comparisons for the biodegradation of thiodiglycol, a product of mustard gas hydrolysis. | an environmentally benign method for the mineralization of sulfur mustard has been proposed involving chemical hydrolysis of sulfur mustard to thiodiglycol, and then the biological degradation of thiodiglycol to generate biomass and gaseous carbon dioxide. alcaligenes xylosoxidans (sh91) was isolated based on its ability to utilize thiodiglycol as a sole carbon source. this article compares different biological reactor designs and experimentally assesses their relative effectiveness in degrading ... | 1996 | 8669917 |
| bioreactor strategies for the treatment of growth-inhibitory waste: an analysis of thiodiglycol degradation, the main hydrolysis product of sulfur mustard. | the microbial degradation of thiodiglycol, the primary hydrolysis product of sulfur mustard, by a pure culture of alcaligenes xylosoxydans ssp. xylosoxydans (sh91) was accomplished in laboratory scale stirred tank reactors. this is a major component of the overall biodegradation process proposed for the complete mineralization of sulfur mustard. several configurations were evaluated for degradation efficiency including batch, repeated batch, continuous stirred tank reactor (cstr), and two-stage ... | 1996 | 8987480 |
| purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by bordetella pertussis and bordetella bronchiseptica. | hydroxamate siderophores of virulent bordetella pertussis and bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(s),11,18(s)- tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone+ ++, which was previously isolated from the taxonomically-related bacterial species alcaligenes denitrificans subsp. xylosoxydans. alcal ... | 1996 | 8744901 |
| achromobacter xylosoxidans bacteremia: report of four cases and review of the literature. | seventy-seven cases of bacteremia due to achromobacter xylosoxidans were reviewed, and susceptibility studies were performed on 11 clinical isolates of a. xylosoxidans. nosocomial bacteremia was noted in 54 of 77 patients (70%), and 28 (36%) had infection associated with an outbreak or acquired from a discrete point source. the most common underlying illnesses were malignancies (30%) and cardiac disease (21%); immunosuppression affected 27%. the most common clinical syndromes were primary and ca ... | 1996 | 8879782 |
| alcaligenes xylosoxidans neonatal meningitis: a case report. | neonatal meningitis caused by alcaligenes xylosoxidans is associated with a high mortality rate. the causative microorganism is resistant to most antimicrobials. generally, once the organism has been isolated from the cerebrospinal fluid of the infected neonate, initial therapy with a third-generation cephalosporin and trimethoprim-sulfamethoxazole is recommended before determining its susceptibility to antimicrobials. there present this is the first of neonatal meningitis with transient diabete ... | 1996 | 8705885 |
| the legionella pneumophila hel locus encodes intracellularly induced homologs of heavy-metal ion transporters of alcaligenes spp. | we continued characterization of the legionella pneumophila hel locus. mutagenesis and dna sequencing identified three genes similar to the czc and cnr loci of alcaligenes eutrophus and the ncc locus of alcaligenes xylosoxidans. on the basis of their similarity to these loci, we designated the l. pneumophila genes helc, helb, and hela. mutations in the hel genes led to reduced cytopathicity towards u937 cells, although the mutant strains did not appear defective in other assays of virulence. tra ... | 1996 | 8613357 |
| [bacteremia caused by alcaligenes (achromobacter) xylosoxidans. description of 3 cases and review of the literature]. | alcaligenes (achromobacter) xylosoxidans occasionally cause infections, mainly in immunocompromised hosts. | 1996 | 8991439 |
| bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of alcaligenes denitrificans rüger and tan 1983. | ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. this species, for which we propose the name bordetella trematum sp. nov., was more closely related to the type species of the genus bordetella (bordetella pertussis) than to the type species of the genus alcaligenes (alcaligenes faecalis) and had t ... | 1996 | 8863408 |
| infection by alcaligenes xylosoxidans subsp. xylosoxidans in neutropenic patients. | alcaligenes xylosoxidans subsp. xylosoxidans (a. x. xylosoxidans) is a nonfermenting gram-negative peritrichous rod and opportunistic pathogen. the organism is frequently found in an aqueous environment. in the past few years, nosocomial infections caused by a. x. xylosoxidans have become more evident. the literature suggests that systemic infections are severe and often lethal and an optimal antibiotic therapy is not well established. this report describes nosocomial infections in 11 patients o ... | 1996 | 8643231 |
| cloning and nucleotide sequence of a d,l-haloalkanoic acid dehalogenase encoding gene from alcaligenes xylosoxidans ssp. denitrificans abiv. | we have cloned dna fragments of plasmid pfl40 from alcaligenes xylosoxidans ssp. denitrificans abiv encoding a d,l-2-haloalkanoic acid halidohydrolase (dhliv). a 6.5-kb ecori/sali-fragment with inducible expression of the halidohydrolase was cloned in pseudomonas fluorescens and escherichia coli. a 1.9-kb hindii-fragment demonstrated expression of the dehalogenase only due to the presence of the promoter from the puc vector in escherichia coli. the nucleotide sequence of this dna-fragment was de ... | 1996 | 9144969 |
| pcr detection of metallo-beta-lactamase gene (blaimp) in gram-negative rods resistant to broad-spectrum beta-lactams. | we applied pcr to the rapid detection of the metallo-beta-lactamase gene, blaimp, in clinically isolated gram-negative rods. a total of 54 high-level ceftazidime-resistant strains (mics, > 128 micrograms/ml) were subjected to pcr analyses with the blaimp-specific primers, since the blaimp-bearing clinical isolates tested in our previous study always demonstrated high-level resistance to ceftazidime. twenty-two blaimp-positive strains including 9 pseudomonas aeruginosa, 9 serratia marcescens, 2 a ... | 1996 | 8940421 |
| molecular epidemiology of burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans in a cystic fibrosis center. | burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans have been isolated with increasing frequency from the sputum of patients with cystic fibrosis in a pediatric hospital. in 1994-95, 27 of 120 patients were persistently colonized, 17 with burkholderia cepacia, eight with alcaligenes xylosoxidans, and five with stenotrophomonas maltophilia. genotyping of 220 clinical isolates revealed that most of the burkholderia cepacia strains were clonally related, suggesting eith ... | 1996 | 8997562 |
| in vitro activities of quinolones, beta-lactams, tobramycin, and trimethoprim-sulfamethoxazole against nonfermentative gram-negative bacilli. | from 1991 to 1995, 8,975 nonfermentative gram-negative bacilli were isolated from patients at the ohio state university medical center: 71% pseudomonas aeruginosa, 14% stenotrophomonas maltophilia, 7.6% acinetobacter baumannii, and < 2% each of 25 other species. the mics of trovafloxacin (cp-99,219), ciprofloxacin, ofloxacin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, ceftazidime, cefoperazone, ceftriaxone, imipenem, tobramycin, and trimethoprim-sulfamethoxazole (tmp-smz) were ... | 1996 | 8726011 |
| bacteremia due to glucose non-fermenting gram-negative bacilli in patients with hematological neoplasias and solid tumors. | twenty-six patients with hematological or solid tumors who developed bacteremia caused by stenotrophomonas maltophilia (n = 10), pseudomonas putida (n = 6), sphingomonas paucimobilis complex (n = 4) or alcaligenes xylosoxidans (n = 6) in the period between 1993 and 1995 were studied. seventeen patients were neutropenic during the infection, and 13 were undergoing bone marrow or peripheral blood stem cell transplantation. twenty-three patients had catheter-related infections; only 3 of the 26 pat ... | 1996 | 8874083 |
| overproduction of d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6 in escherichia coli and its purification. | we constructed the high-expression plasmid for d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6. the appropriate shine-dalgarno sequence (aaggag) was introduced to the eight bases upstream of start codon (atg) of d-aminoacylase structural gene by site-directed mutagenesis, and then the 1.75-kb dna fragment including the open reading frame was inserted into the downstream of the tac promoter of plasmid vector pkk223-3. the resultant plasmid, which was named pknsd2, showed a hi ... | 1996 | 8776758 |
| alcaligenes xylosoxidans subsp xylosoxidans in children with chronic otorrhea. | because other known pathogens are frequently isolated with axx, its clinical significance may be overlooked. axx should not be considered a colonizer or contaminant, particularly in the presence of clinical signs and symptoms of infection. for a pediatric patient with axx in the ear fluid, the choice of an antibiotic regimen should be based on in vitro activity of both axx and other concurrent pathogens. although the sensitivity of axx may be highly variable, a number of antibiotics appear to be ... | 1996 | 8637765 |
| homologous plasmids from soil bacteria encoding d,l-halidohydrolases | we isolated and characterized d,l-halidohydrolases fromfive different soil bacteria. three of these bacterial strains bear plasmidswith sizes of approximately 60 kb. curing and mating experiments indicatedthat these three plasmids pfl160, pfl170, and pfl190 encoded a dehalogenase.owing to their biochemical characterization, these halidohydrolases wereclosely related among each other and to the dhliv halidohydrolase, encoded byplasmid pfl40 from alcaligenes xylosoxidans ssp.denitrificans abiv. re ... | 1997 | 9003586 |
| identification of burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. | we evaluated 819 isolates referred to us as "burkholderia cepacia" from cystic fibrosis (cf) clinics and research laboratories from five countries; 28 (3.4%) were not b. cepacia. a further 12 (1.5%) organisms appeared to be other burkholderia species, but identification could not be confirmed by conventional means. the most prevalently misidentified organisms were stenotrophomonas maltophilia, alcaligenes xylosoxidans, and comamonas acidovorans. many of these organisms grew on oxidation-fermenta ... | 1997 | 9041399 |
| nitrite reductase from achromobacter xylosoxidans forms stable trimers in dilute solution. | 1997 | 9056938 | |
| comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods. | in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ... | 1997 | 9210669 |
| microbial contamination of antiseptic-soaked cotton balls. | we investigated microbial contamination of in-use antiseptics at a hospital. no microbial contamination was observed in 70 samples of 0.02% benzalkonium chloride solution (500-ml volume), 70 samples of 1% titratable i2 povidone-iodine solution (250-ml volume), or 15 samples of 0.1% ethacridine lactate solution (500-ml volume) during use in reduced amounts. nor was any microbial contamination observed in 70 samples of cotton balls soaked in 1% titratable i2 povidone-iodine solution in canisters o ... | 1997 | 9212987 |
| [bacteremia by alcaligenes xylosoxidans: report of a case]. | 1997 | 9289534 | |
| comparison of polymerase chain reaction and pulsed-field gel electrophoresis for the epidemiological typing of alcaligenes xylosoxidans subsp. xylosoxidans in a burn unit. | eighteen isolates of alcaligenes xylosoxidans subsp. xylosoxidans were collected from clinical specimens of 15 patients in a burn unit and a plastic surgery ward over a 16-month period. pulsed-field gel electrophoresis and polymerase chain reaction (pcr) were compared for the epidemiologic typing of these 18 isolates and fifteen epidemiologically unrelated strains. these 18 isolates demonstrated an identical fingerprint pattern and were easily distinguished from the 15 epidemiologically unrelate ... | 1997 | 9327244 |
| colonization by alcaligenes xylosoxidans in children with cystic fibrosis: a retrospective clinical study conducted by means of molecular epidemiological investigation. | 1997 | 9114166 | |
| evidence for the genetic unrelatedness of nosocomial alcaligenes xylosoxidans strains in a pediatric hospital. | restriction fragment-length polymorphism of ribosomal dna and random amplified polymorphic dna analysis were used to study the relationships among nine isolates of alcaligenes xylosoxidans obtained from seven children in a pediatric hospital. all the children harbored only genotypically unrelated strains, thus excluding a common source of exposure or patient-to-patient strain transfer. | 1997 | 9120242 |
| [neonatal meningitis caused by alcaligenes xylosoxydans]. | neonatal meningitis due to alcaligenes xylosoxydans is exceptional; its diagnosis and treatment may be difficult. | 1997 | 9181021 |
| ph-dependence for binding a single nitrite ion to each type-2 copper centre in the copper-containing nitrite reductase of alcaligenes xylosoxidans. | the first quantitative characterization of the interaction of no2(-) with the cu-containing dissimilatory nitrite reductase (nir) of alcaligenes xylosoxidans using steady-state kinetics, equilibrium gel filtration and epr spectroscopy is described. each molecule of this protein consists of three equivalent subunits, each containing a type-1 cu atom and also a type-2 cu atom at each subunit interface. enzyme activity increased in a biphasic manner with decreasing ph, having an optimum at ph 5.2 a ... | 1997 | 9182711 |
| characterization of the gene encoding catechol 2,3-dioxygenase from achromobacter xylosoxidans kf701. | catechol 2,3-dioxygenase (c23o) catalyzes a meta cleavage of the aromatic ring in catechol to form 2-hydroxymuconic semialdehyde. a c23o gene was cloned from chromosomal dna of a. xylosoxidans kf701, a soil bacterium degrading biphenyl, and expressed in e. coli hb101. in substrate specificity to catechol and its analogs, the c23o exhibited the highest aromatic ring-fission activity to catechol, and its relative activity to other dihydroxylated aromatics was 4-chlorocatechol > 4-methylcatechol > ... | 1997 | 9299526 |
| bacteremia and respiratory involvement by alcaligenes xylosoxidans in patients infected with the human immunodeficiency virus. | seven cases of alcaligenes xylosoxidans bacteremia and/or respiratory disease in patients infected with the human immunodeficiency virus (hiv) are described. reported only thrice previously in this setting, these bacterial complications occurred during different phases of hiv infection and were associated with leukopenia-neutropenia in four patients and a central vascular catheter in two. although the majority of cases were diagnosed after day 3 of hospitalization, a distinct source of infection ... | 1997 | 9495677 |
| structures of a blue-copper nitrite reductase and its substrate-bound complex. | copper-containing nitrite reductases (nir's) have been conveniently subdivided into blue and green nir's which are thought to be redox partners of azurins and pseudo-azurins, respectively. crystal structures of two green nir's have recently been determined. alcaligenes xylosoxidans has been shown to have a blue-copper nitrite reductase (axnir) and two azurins with 67% homology both of which donate electrons to it effectively. the first crystal structure of a blue nir (axnir) in its oxidized and ... | 1997 | 15299906 |
| observations of metabolite formation and variable yield in thiodiglycol biodegradation process : impact on reactor design. | the complete microbial degradation of thiodiglycol (tdg), the primary hydrolysis product of sulfur mustard, by alcaligenes xylosoxydans ssp. xylosoxydans (sh91) was accomplished in laboratory-scale stirredtank reactors. an andrews substrate inhibition model was used to describe the cell growth. the yield factor was not constant, but a relationship with initial substrate concentration has been developed. using a substrate-inhibition and variable-yield kinetic model, we can describe the cell growt ... | 1997 | 18576129 |
| crystallization and preliminary x-ray studies of azurin-i and azurin-ii from denitrifying bacterium alcaligenes xylosoxidans gifu 1051. | two distinct azurins named azurin-i and azurin-ii were isolated from alcaligenes xylosoxidans gifu 1051 (azg-i and azg-ii). they have been purified and crystallized. the crystals of azg-i belong to the monoclinic crystal system, space group c2, unit-cell parameters a = 130.6, b = 54.4, c = 74.7 a, beta = 96.1 degrees. the crystals of azg-ii belong to the tetragonal crystal system, space group p4(1)22 or p4(3)22, unit-cell parameters a = b = 52.6, c = 100.7 a. both crystals diffract up to 2.0 a r ... | 1997 | 15299941 |
| achromobacter xylosoxidans bacteremia in patients with hematologic malignancies. | we report nine cases of achromobacter xylosoxidans bacteremia diagnosed in patients with hematologic malignancies. there was not an obvious epidemiologic link between cases and the organism was not isolated from any source. outcome was cure in all nine cases. in our experience, catheter removal is generally required for eradication of a. xylosoxidans. | 1998 | 9573685 |
| genetic structure of the bphg gene encoding 2-hydroxymuconic semialdehyde dehydrogenase of achromobacter xylosoxidans kf701. | 2-hydroxymuconic semialdehyde dehydrogenase catalyzes the conversion of 2-hydroxymuconic semialdehyde (hms) to an enol form of 4-oxalocrotonate which is a step in the catechol meta-cleavage pathway. a bphg gene encoding hms dehydrogenase of a. xylosoxidans kf701, a soil bacterium degrading biphenyl, was identified at between catechol 2,3-dioxygenase gene and hms hydrolase gene, and its sequence was analyzed. an open reading frame (orf) corresponding to bphg gene was consisted of 1461 nucleotides ... | 1998 | 9600061 |
| the covalent structure of the blue copper-containing nitrite reductase from achromobacter xylosoxidans. | the complete amino acid sequence of the blue copper-containing nitrite reductase enzyme (nir) from achromobacter xylosoxidans has been determined by chemical analysis, supported by high precision mass analysis. the polypeptide chain contains 336 residues with an overall charge of 0, including the +2 state of each of the copper ions. the two nir enzymes for which the three-dimensional structures have been solved are green in color and have different absorption spectra than those of the blue-color ... | 1998 | 9647763 |
| emendation of genus achromobacter and achromobacter xylosoxidans (yabuuchi and yano) and proposal of achromobacter ruhlandii (packer and vishniac) comb. nov., achromobacter piechaudii (kiredjian et al.) comb. nov., and achromobacter xylosoxidans subsp. denitrificans (rüger and tan) comb. nov. | based on the results of gc content determination and 16s rrna sequence analysis among the type strains of achromobacter xylosoxidans, 4 alcaligenes species, 5 bordetella species, and 12 species of 4 other genera, the separation of genus achromobacter yabuuchi and yano 1981, with the type species achromobacter xylosoxidans, is confirmed. alcaligenes ruhlandii (packer and vishniac) aragno and schlegel 1992 is a distinct species and not a senior synonym of achromobacter xylosoxidans. alcaligenes ru ... | 1998 | 9688077 |
| the intramolecular electron transfer between copper sites of nitrite reductase: a comparison with ascorbate oxidase. | the intramolecular electron transfer (et) between the type 1 cu(i) and the type 2 cu(ii) sites of alcaligenes xylosoxidans dissimilatory nitrite reductase (axnir) has been studied in order to compare it with the analogous process taking place in ascorbate oxidase (ao). this internal process is induced following reduction of the type 1 cu(ii) by radicals produced by pulse radiolysis. the reversible et reaction proceeds with a rate constant ket = k(1-->2) + k(2-->1) of 450 +/- 30 s(-1) at ph 7.0 a ... | 1998 | 9781686 |
| structure of azurin i from the denitrifying bacterium alcaligenes xylosoxidans ncimb 11015 at 2.45 a resolution. | azurin i from alcaligenes xylosoxidans ncimb 11015 (azn-i) was crystallized by using peg 4000 as a precipitant. the crystals belong to the monoclinic crystal system and have a space group c2 with the unit-cell parameters of a = 130.67, b = 54.26, c = 74.55 a, and beta = 95.99 degrees. the structure of azn-i has been solved by the molecular replacement method. azurin ii from the same bacterium (azn-ii) was chosen as the initial structural model. the final crystallographic r value is 17.3% and fre ... | 1998 | 9761902 |
| microbiology of sputum from patients at cystic fibrosis centers in the united states. | during a phase iii national collaborative study of aerosolized tobramycin (1 july 1995 through 30 september 1996), the microbiology of specimens from 595 patients at 69 cystic fibrosis (cf) centers was examined. samples from three screening visits were processed in a single laboratory by means of standardized techniques for identification and susceptibility testing. from 1,753 pretreatment specimens, 5,128 pathogens were isolated (average, 2.9/specimen). of the 3,936 pseudomonas aeruginosa isola ... | 1998 | 9675470 |
| persistent airway colonization with alcaligenes xylosoxidans in two brothers with cystic fibrosis. | 1998 | 9629983 | |
| [alcaligenes xylosoxydans: the origin of infection is most often an antiseptic solution]. | 1998 | 9809170 | |
| matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry analysis of blue copper proteins. azurin and mavicyanin. | two copper proteins azurin-1 and azurin-2 were isolated from denitrifying bacteria alcaligenes xylosoxidans gifu1051, and the mass spectrometric analysis of the proteins were carried out by both matrix-assisted laser desorption ionization time-of-flight (maldi-tof) and electrospray ionization (esi). the mass spectrometric analysis was also carried out with the recombinant zucchini protein mavicyanin, which was obtained by expression in escherichia coli. all the proteins were detected as positive ... | 1998 | 9842744 |
| [two cases of achromobacter xylosoxidans sepsis]. | achromobacter xylosoxidans is a gram-negative bacterium whose natural habitat has not been clearly defined. it has been isolated from ear discharge and the large intestine of humans and from various hospital or environmental water sources. infection with a. xylosoxidans in humans has been documented, and resulting illnesses include meningitis, pneumonia, cholecystitis, peritonitis and urinary tract infection. bacteremia due to a. xylosoxidans is rare, and little information on treatment is avail ... | 1998 | 9847526 |
| type 1 cu structure of blue nitrite reductase from alcaligenes xylosoxidans gifu 1051 at 2.05 a resolution: comparison of blue and green nitrite reductases. | the crystal structure of the blue nitrite reductase from alcaligenes xylosoxidans gifu 1051 (axgnir) has been determined at 2.05 a resolution. axgnir contains both type 1 and 2 cu sites, the geometry of the former being distorted tetrahedral. the superpositioning of the type 1 cu sites in the blue enzyme and a green nitrite reductase revealed that the orientation of the met150 side chain differed. the deviation of the sdelta(met150) atom from the axial position of the nns plane formed by two nde ... | 1998 | 9792907 |
| investigation of an outbreak of wound infections due to alcaligenes xylosoxidans transmitted by chlorhexidine in a burns unit. | alcaligenes xylosoxidans, an environmental gram-negative bacillus, was isolated within a 1-month period from six patients in a pediatric burns unit. twelve isolates were studied, one from each of the six patients (five from wound cultures and one from a blood culture) and one from each of six contaminated atomizers containing chlorhexidine diluted to 600 mg/l. the biochemical and susceptibility patterns of all the isolates were similar, and their dna enzyme restriction patterns were identical. t ... | 1998 | 9865987 |
| x-ray structure of a blue-copper nitrite reductase in two crystal forms. the nature of the copper sites, mode of substrate binding and recognition by redox partner. | denitrification is one of the main steps of the global nitrogen cycle that is sustained by prokaryotic organisms. denitrifying bacteria use two entirely different enzymes in this process, one based on haem cd1 prosthetic groups and the other on type 1-type 2 cu centres. copper-containing nitrite reductases (nirs) are sub-divided into blue and green nirs, which are respectively thought to be redox partners of azurins and pseudo-azurins. crystallographic structures of the blue nitrite reductase fr ... | 1998 | 9735294 |
| peritoneal dialysis-associated peritonitis caused by alcaligenes xylosoxidans. | despite significant progress to decrease its incidence, peritonitis remains the main source of morbidity and treatment failure in patients on continuous ambulatory peritoneal dialysis (capd). the majority of cases of peritonitis result from infection with aerobic gram-positive (staphylococcus epidermidis and staphylococcus aureus), or gram-negative organisms. less common organisms that are also reported include anaerobic bacteria, fungi, and mycobacteria, which collectively account for less than ... | 1998 | 9730575 |
| alcaligenes xylosoxidans keratitis post penetrating keratoplasty in a rigid gas permeable lens wearer. | we report a case of alcaligenes xylosoxidans keratitis following penetrating keratoplasty in a rigid gas permeable (rgp) lens wearer. | 1998 | 9800065 |
| [case report: subcutaneous abscess and thoracic empyema caused by alcaligenes xylosoxidans]. | alcaligenes xylosoxidans is a glucose-nonfermentative gram-negative rod which usually exists in the environment. this organism while causing pneumonia, sepsis, meningitis and urinary tract infection in the compromised host, rarely causes thoracic empyema. we report a case of thoracic empyema and subcutaneous abscess due to a. xylosoxidans. a 74-year-old male, who had undergone right total pneumonectomy for chronic necrotizing pulmonary aspergillosis a year ago, was admitted to our hospital becau ... | 1998 | 9695474 |
| epidemiological typing of alcaligenes xylosoxidans subsp. xylosoxidans by antibacterial susceptibility testing, fatty acid analysis, page of whole-cell protein and pulsed-field gel electrophoresis. | antibacterial susceptibility testing, fatty acid analysis, protein analysis and dna analysis of alcaligenes xylosoxidans subsp. xylosoxidans were compared to determine the efficiency of the methods available for strain typing. thirty isolates were investigated: 20 clinical isolates from a nonsocomial outbreak in essen (germany), 9 clinical isolates from sporadic nosocomial cases in paris (france) and reference strain atcc 2402. the highest microbiological discriminative power was exhibited by pu ... | 1998 | 9728414 |
| a pseudoepidemic of alcaligenes xylosoxidans attributable to contaminated saline. | alcaligenes xylosoxidans is an uncommon but serious cause of nosocomial epidemics. this report describes a cluster of two patients who underwent revision of hip arthroplasties and one patient who had a lumbar puncture. cultures obtained during all three procedures showed a. xylosoxidans with similar antibiotic sensitivity patterns. an investigation found that saline used to process these specimens was contaminated with this organism. | 1998 | 9584810 |
| [occurrence of gram-negative non-fermenting rods in hemocultures and their sensitivity to antimicrobial agents]. | in the period from january 1993 to june 1996 were at the department of microbiology of the university hospital in olomouc 122 strains of gram-negative nonfermentative rod-shaped bacteria isolated from haemocultures. the majority represented the group of 51 strains of the genus acinetobacter (41.8%), complex a. calcoaceticus-baumannii (acb complex). the second largest group were 21 strains (17.2%) of pseudomonas aeruginosa. these were followed by 17 strains (13.9%) of stenotrophomonas maltophilia ... | 1998 | 9919762 |
| [evaluation of antibacterial activities of various antibiotics against glucose non-fermentative gram-negative rods other than pseudomonas aeruginosa]. | mics of piperacillin, sulbactam/cefoperazone, minocycline (mino), gentamicin, amikacin, flomoxef, ceftazidime, cefozopran, cefsulodin and imipenem were determined, against 189 clinical isolated strains of glucose non-fermentative gram-negative rods (nfgnr; acinetobacter baumannii (44), alcaligenes faecalis (5), alcaligenes xylosoxidans (25), burkholderia cepacia (12), chryseobacterium indologenes (23), chryseobacterium meningosepticum (9), pseudomonas fluorescens (8), pseudomonas putida (12), st ... | 1998 | 9755431 |
| development of pcr primer systems for amplification of nitrite reductase genes (nirk and nirs) to detect denitrifying bacteria in environmental samples. | a system was developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with pcr. primer sequences were found for the amplification of fragments from both nitrite reductase genes (nirk and nirs) after comparative sequence analysis. whenever amplification was tried with these primers, the known nir type of denitrifying laboratory cultures could be confirmed. likewise, the method allowed a determination of the nir type of five laboratory ... | 1998 | 9758798 |
| crystallographic study of azurin from pseudomonas putida. | azurin from pseudomonas putida is a blue copper protein which functions as an electron carrier. two crystal forms of azurin were grown, one in the presence and the other in the absence of zinc acetate; each belongs to space group p21 and contains two molecules per asymmetric unit. the zinc-free crystals have cell dimensions a = 43.25, b = 50.65, c = 54.60 a, beta = 107.79 degrees, while the crystals grown from zinc-containing solution have cell dimensions a = 40.76, b = 51.22, c = 54.96 a, beta ... | 1998 | 9761890 |
| crystallization and preliminary x-ray study of iso-2 azurin from the methylotrophic bacterium, methylomonas j. | the obligate methylotroph methylomonas j possesses two distinct azurins. the iso-2 azurin, which functions as an electron acceptor for methylamine dehydrogenase, has been crystallized using two kinds of precipitants: peg 4000 and ammonium sulfate. the crystals precipitated with peg belong to the monoclinic system, space group p21, with unit-cell parameters a = 32.96, b = 33.67, c = 47.34 a and beta = 101.35 degrees. the crystals precipitated with ammonium sulfate belong to the orthorhombic syste ... | 1999 | 10089434 |
| structural and kinetic evidence for an ordered mechanism of copper nitrite reductase. | the crystallographic structures of several copper-containing nitrite reductases are now available. despite this wealth of structural data, no definitive information is available as to whether the reaction proceeds by an ordered mechanism where nitrite binds to the oxidised type 2 site, followed by an internal electron transfer from the type 1 cu, or whether binding occurs to the reduced type 2 cu centre, or a random mechanism operates. we present here the first structural information on both typ ... | 1999 | 10222206 |
| biochemical characterization and solution structure of nitrous oxide reductase from alcaligenes xylosoxidans (ncimb 11015). | nitrous oxide reductase (n2or) is the terminal enzyme involved in denitrification by microbes. no three-dimensional structural information has been published for this enzyme. we have isolated and characterised n2or from alcaligenes xylosoxidans (axn2or) as a homodimer of m(r) 134,000 containing seven to eight copper atoms per dimer. comparison of sequence and compositional data with other n2ors suggests that axn2or is typical and can be expected to have similar domain folding and subunit structu ... | 1999 | 10092849 |
| amplified rdna restriction analysis and further genotypic characterisation of metal-resistant soil bacteria and related facultative hydrogenotrophs. | the level of genotypic relationship between czc+ soil bacteria mainly resistant to zinc (but also to various other metals), and related facultative hydrogenotrophs previously assigned to the genera alcaligenes, ralstonia, and burkholderia was evaluated using ardra (amplified ribosomal dna restriction analysis). the analysis included 44 strains isolated from harsh industrial environments in sediments, soils and wastes with high content of heavy metals. these strains were selected by their ability ... | 1999 | 10390877 |
| the ph-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. | electron transfer over 12.6 a from the type 1 copper (t1cu) to the type 2 copper (t2cu) was investigated in the copper-containing nitrite reductases from two denitrifying bacteria (alcaligenes xylosoxidans gifu 1051 and achromobacter cycloclastes ian 1013), following pulse radiolytical reduction of t1cu. in the presence of nitrite, the rate constant for the intramolecular electron transfer of the enzyme from a. xylosoxidans decreased 1/2 fold to 9 x 10(2) s-1 (20 degrees c, ph 7.0) as compared t ... | 1999 | 10423537 |
| [neonatal meningitis due to alcaligenes xylosoxidans contaminating aqueous solution of eosin]. | 1999 | 10079896 |