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enhanced chrysene degradation by halotolerant achromobacter xylosoxidans using response surface methodology.degradation of chrysene, a four ring high molecular weight (hmw) polycyclic aromatic hydrocarbon (pah) is of intense environmental interest, being carcinogenic, teratogenic and mutagenic. multiple pah degrading halotolerant achromobacter xylosoxidans was isolated from crude oil polluted saline site. response surface methodology (rsm) using central composite design (ccd) of bushnell-haas medium components was successfully employed for optimization resulting 40.79% chrysene degradation on 4th day. ...201121855331
fluorescence lifetime analysis of nitrite reductase from alcaligenes xylosoxidans at the single-molecule level reveals the enzyme mechanism. 201121922585
alcaligenes xylosoxidans contact lens-related keratitis--a case report and literature review.alcaligenes xylosoxidans can result in opportunistic infections in patients who are immunocompromised. alcaligenes xylosoxidans keratitis is a rare infection, almost always occurring in a compromised cornea. few reported cases have occurred as contact lens-induced keratitis. the purposes of this report are to report a case of a. xylosoxidans keratitis in an immunocompetent contact lens wearer (2-week disposable) and to review the related literature.201121993586
Achromobacter xylosoxidans mesh related infection: A case of delayed diagnosis and management.We present the first case of mesh related infection caused by Achromobacter xylosoxidans after ventral hernia repair. After repair of a small paraumbilical hernia, the postoperative course was complicated by persistent discharging sinuses despite the removal of underlying polypropylene mesh. Removal of an intrabdominal omental inflammatory mass containing pus that showed growth of A. xylosoxidans led to the resolution of all the symptoms.201121959012
development of an immunochromatographic assay for diagnosing the production of imp-type metallo-β-lactamases that mediate carbapenem resistance in pseudomonas.rapid and reliable detection of carbapenem-resistant bacteria is an important infection-control measure and a crucial aspect of antimicrobial chemotherapy. imp-type metallo-β-lactamase (mbl) is an emzyme that mediate carbapenem resistance in bacteria. here, an immunochromatographic assay was newly developed using novel monoclonal antibodies (mabs) recognizing imp-type mbl. epitope mapping of mabs and mutational analysis of the epitope region in imp antigen suggested that the mabs could react to ...201121986031
Distribution of microbial biomass and the potential for anaerobic respiration in Hanford Site 300 Area subsurface sediment.Subsurface sediments were recovered from a 52 m deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the oxic coarse-grained Hanford formation (9-17.4 m), the oxic fine-grained upper Ringold Formation (17.7-18.1 m), and the reduced Ringold Formation (18.3-52m). Microbial biomass (measu ...201122138990
heterotrophic nitrification by achromobacter xylosoxidans s18 isolated from a small-scale slaughterhouse wastewater.heterotrophic carbon utilizing microbes were acclimatized in the laboratory by inoculating sludge collected from the waste discharge pond of a small-scale rural abattoir in india in a nutrient solution intermittently fed with glucose and ammonium chloride. cultures of 10 well-developed isolates were selected and grown in a basal medium containing glucose and ammonium chloride. culture supernatants were periodically analyzed for ammonium nitrogen (nh(4) (+)-n) and c ...201122130566
achromobacter species endocarditis: a case report and literature review.endocarditis due to achromobacter species is a rare, yet serious, endovascular infection. achromobacter species infective endocarditis is associated with underlying immunodeficiencies or prosthetic heart valves and devices. a case of prosthetic pulmonary valve endocarditis secondary to achromobacter xylosoxidans subspecies denitrificans is described in the present report. this life-threatening infection was successfully treated with combined valve replacement and prolonged antibiotic therapy. a ...201122942890
mexxy multidrug efflux system of pseudomonas aeruginosa.anti-pseudomonas aminoglycosides, such as amikacin and tobramycin, are used in the treatment of pseudomonas aeruginosa infections. however, their use is linked to the development of resistance. during the last decade, the mexxy multidrug efflux system has been comprehensively studied, and numerous reports of laboratory and clinical isolates have been published. this system has been increasingly recognized as one of the primary determinants of aminoglycoside resistance in p. aeruginosa. in p. aer ...201223233851
genetic and biochemical characterization of a novel metallo-β-lactamase, tmb-1, from an achromobacter xylosoxidans strain isolated in tripoli, libya.an achromobacter xylosoxidans strain from the tripoli central hospital produced a unique metallo-β-lactamase, designated tmb-1, which is related to dim-1 (62%) and gim-1 (51%). bla(tmb-1) was embedded in a class 1 integron and located on the chromosome. the tmb-1 β-lactamase has lower k(cat) values than both dim-1 and gim-1 with cephalosporins and carbapenems. the k(m) values were more similar to those of gim-1 than those of dim-1, with the overall k(cat)/k(m) values being lower than those for g ...201222290947
achromobacter piechaudii bloodstream infection in an immunocompetent host.achromobacter piechaudii is a rare cause of clinical disease in humans. previously, clinical disease has only been documented in immunocompromised patients. we present a case of achromobacter piechaudii bacteremia in a patient with previous malignancy but no known immunosuppression.201223569545
a multilocus sequence typing scheme implies population structure and reveals several putative novel achromobacter species.the genus achromobacter currently is comprised of seven species, including achromobacter xylosoxidans, an opportunistic and nosocomial pathogen that displays broad-spectrum antimicrobial resistance and is recognized as causing chronic respiratory tract infection in persons with cystic fibrosis (cf). to enable strain typing for global epidemiologic investigations, to clarify the taxonomy of "achromobacter-like" strains, and to elucidate the population structure of this genus, we developed a genus ...201222785192
multilocus sequence analysis of isolates of achromobacter from patients with cystic fibrosis reveals infecting species other than achromobacter xylosoxidans.a multilocus sequence analysis (mlsa) scheme was developed for characterization of strains and species from the genus achromobacter, which are increasingly recovered from patients with cystic fibrosis (cf). five conserved housekeeping genes were selected for the mlsa, which was applied to a diverse collection of 77 strains originating from europe, asia, and south america and including type strains of the seven recognized achromobacter species, six environmental strains, eight non-cf clinical str ...201222675125
investigation and control of an outbreak of achromobacter xylosoxidans bacteremia.objective. to define the extent of an outbreak of achromobacter xylosoxidans bacteremia, determine the source of the outbreak, and implement control measures. design. an outbreak investigation, including environmental and infection control assessment, and evaluation of hypotheses using the binomial distribution and case control studies. setting. a 50-bed medical surgical unit in a hospital in illinois during the period january 1-july 15, 2006. interventions. discontinuation of use of opioid d ...201222227988
[strain alcaligenes xylosoxydans subsp. denitrificans td2 as a biosensor basis for determination of thiodiglycol]. 201223610933
clinical and microbiological evaluation of eight patients with isolated achromobacter xylosoxidans.achromobacter xylosoxidans causes infections in patients with underlying disorders. the aim of this study was to evaluate achromobacter xylosoxidans cases. eight patients were included, 5 infections and 3 colonizations. three of them survived and 2 died. the predisposing factor was catheter. infection control measures could prevent infection.201222404460
synthesis, characterization, and antimicrobial activity of silver carbene complexes derived from 4,5,6,7-tetrachlorobenzimidazole against antibiotic resistant bacteria.silver n-heterocyclic carbene complexes have been shown to have great potential as antimicrobial agents, affecting a wide spectrum of both gram-positive and gram-negative bacteria. a new series of three silver carbene complexes (sccs) based on 4,5,6,7-tetrachlorobenzimidazole has been synthesized, characterized, and tested against a panel of clinical strains of bacteria. the imidazolium salts and their precursors were characterized by elemental analysis, mass spectrometry, (1)h and (13)c nmr spe ...201222402409
in vitro antimicrobial activity of "last-resort" antibiotics against unusual nonfermenting gram-negative bacilli clinical isolates.in this prospective multicentric study, we assessed the in vitro antimicrobial activity of carbapenems (imipenem, meropenem, and doripenem), tigecycline, and colistin against 166 unusual nonfermenting gram-negative bacilli (nf-gnb) clinical isolates collected from nine french hospitals during a 6-month period (from december 1, 2008, to may 31, 2009). all nf-gnb isolates were included, except those phenotypically identified as pseudomonas aeruginosa or acinetobacter baumannii. minimal inhibitory ...201222335615
achromobacter xylosoxidans keratitis after contact lens usage.to report on achromobacter xylosoxidans keratitis in two healthy patients who had worn contact lenses foran extended period of time. a 36-year-old female and a 21-year-old female visited our hospital with ocular pain and blurred vision. both patients had a history of wearing soft contact lenses for over fve years with occasional overnight wear. at the initial presentation, a slit lamp examination revealed corneal stromal infiltrations and epithelial defects with peripheral neovascularization in ...201222323886
reductive dechlorination of methoxychlor by bacterial species of environmental origin: evidence for primary biodegradation of methoxychlor in submerged environments.methoxychlor [1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane] is an organochlorine insecticide that undergoes dechlorination in natural submerged environments. we investigated the ability to dechlorinate this compound in seven environmental bacterial species ( aeromonas hydrophila , enterobacter amnigenus , klebsiella terrigena , bacillus subtilis , achromobacter xylosoxidans , acinetobacter calcoaceticus , and mycobacterium obuense ) and the enteric bacterium escherichia coli as a positive cont ...201222292429
calcaneal osteomyelitis due to achromobacter xylosoxidans: a case report.achromobacter xylosoxidans (a. xylosoxidans) has been described as an opportunistic pathogen causing infection. the case we describe is that of an elderly man who had osteomyelitis of calcaneal bone caused by a. xylosoxidans. as far as we are aware there are only 5 cases of osteomyelitis with a. xylosoxidans in the literature. impaired defensive mechanism of the foot in direct contact with this waterborne bacterium can cause this disease. because of the high level of antibiotic resistance of thi ...201222286410
new treatments for emerging cystic fibrosis pathogens other than pseudomonas.the development of antimicrobial treatments for respiratory pathogens in cystic fibrosis (cf) has been an integral component to the increased survival of cf patients over the past fifty years. despite significant treatment advances, however, respiratory failure secondary to chronic bacterial pulmonary infection remains the primary cause of death in cf patients. the purpose of this review is to discuss emerging pathogens (other than pseudomonas) in cf by describing the characteristics of the orga ...201222229574
[achromobacter xylosoxidans]. 201223096549
[a case obstructive jaundice and liver abscess caused by achromobacter xylosoxidans]. 201223320273
[case report: respiratory infection due to alcaligenes xylosoxidans in a patient with mounier-kuhn syndrome].mounier-kuhn syndrome is a rare entity characterized by abnormal dilatation of the trachea and main bronchi (tracheobronchomegaly). alcaligenes xylosoxidans is a non fermenting gram-negative pathogen common in extra-and intra-hospital environment, which may be related to immunosuppression states. we describe the case of a 75 years old male, ex-smoker with moderate functional obstruction, chronic respiratory failure and chronic colonization by pseudomonas aeuriginosa. he had an infectious exacerb ...201223282506
community acquired bilateral upper lobe pneumonia with acute adrenal insufficiency: a new face of achromobacter xylosoxidans.achromobacter xylosoxidans is an uncommon pathogen of low virulence known to cause serious nosocomial infection in the immunocompromised. its inherent multi-drug resistance makes treatment difficult. community-acquired infections are rare despite its ubiquitous existence. we present a 50-year-old immunocompetent woman who presented with one-month history of coughing with expectoration who was subsequently diagnosed with bilateral upper lobe pneumonia and acute adrenal insufficiency. achromobacte ...201223173016
achromobacter xylosoxidans: an emerging pathogen carrying different elements involved in horizontal genetic transfer.in the last few years, numerous cases of multidrug-resistant achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. to gain insights into the molecular mechanisms and mobile elements related to multidrug resistance in this bacterium, we studied 24 non-epidemiological a. xylosoxidans clinical isolates from argentina. specific primers for plasmids, transposons, insertion sequences, bla(ampc), inti1, and inti2 genes were used in pcr reactions. ...201222926720
microbial contamination of a disinfectant-soaked unwoven cleaning cloth.in december 2009, a 76-year-old male patient developed pneumonia due to burkholderia cepacia whilst in an intensive care unit at a japanese university hospital. during the subsequent environmental investigation to find the source, b. cepacia with an identical dna type was found in his denture storage solution. open packets of unwoven rayon cloths soaked in 0.2% alkyldiaminoethylglycine hydrochloride, used for environmental cleaning, were shown to be contaminated with b. cepacia, alcaligenes xylo ...201222854353
1-aminocyclopropane-1-carboxylic acid (acc) deaminase-containing rhizobacteria protect ocimum sanctum plants during waterlogging stress via reduced ethylene generation.ocimum sanctum grown as rain-fed crop, is known to be poorly adapted to waterlogged conditions. many a times the crop suffers extreme damages because of anoxia and excessive ethylene generation due to waterlogging conditions present under heavy rain. the usefulness of 1-aminocyclopropane-1-carboxylic acid (acc) deaminase-containing plant growth promoting rhizobacteria was investigated under waterlogging stress. the comparison of herb yield and stress induced biochemical changes of waterlogged an ...201222846334
continuous ambulatory peritoneal dialysis-related peritonitis in an uremic outpatient: achromobacter xylosoxidans. 201222814104
electrochemical single-molecule afm of the redox metalloenzyme copper nitrite reductase in action.we studied the electrochemical behavior of the redox metalloenzyme copper nitrite reductase (cnir, achromobacter xylosoxidans) immobilized on a au(111)-electrode surface modified by a self-assembled cysteamine molecular monolayer (sam) using a combination of cyclic voltammetry and electrochemically-controlled atomic force microscopy (in situ afm). the enzyme showed no voltammetric signals in the absence of nitrite substrate, whereas a strong reductive electrocatalytic signal appeared in the pres ...201222696401
the impact of copper, nitrate and carbon status on the emission of nitrous oxide by two species of bacteria with biochemically distinct denitrification pathways.denitrifying bacteria convert nitrate (no(3) (-) ) to dinitrogen (n(2) ) gas through an anaerobic respiratory process in which the potent greenhouse gas nitrous oxide (n(2) o) is a free intermediate. these bacteria can be grouped into classes that synthesize a nitrite (no(2) (-) ) reductase (nir) that is solely dependent on haem-iron as a cofactor (e.g. paracoccus denitrificans) or a nir that is solely dependent on copper (cu) as a cofactor (e.g. achromobacter xylosoxidans). regardless of which ...201222642644
molecular characterization of imp-type metallo-β-lactamases among multidrug-resistant achromobacter xylosoxidans.to analyse the metallo-β-lactamase (mbl) genes and their genetic environments in clinical isolates of achromobacter xylosoxidans.201222577105
infective endocarditis caused by achromobacter xylosoxidans: a case report and review of the literature.an 86-year-old woman who underwent placement of a prosthetic aortic valve for regurgitation 5 years previously was admitted because of spiking fever. the blood culture results were positive for gram-negative rods, which were identified as achromobacter xylosoxidans. approximately 4 months after being sent to the hospital, transthoracic echocardiography revealed vegetation at the prosthetic aortic valve. ultimately, a diagnosis of a. xylosoxidans endocarditis of the prosthetic aortic valve was ma ...201222576403
laser-flash photolysis indicates that internal electron transfer is triggered by proton uptake by alcaligenes xylosoxidans copper-dependent nitrite reductase.enzyme-catalysed electron transfer reactions are often controlled by protein motions and coupled to chemical change such as proton transfer. we have investigated the nature of this control in the blue copper-dependent nitrite reductase from alcaligenes xylosoxidans (axnir). inter-cu electron transfer from the t1cu site to the t2cu catalytic site in axnir occurs via a proton-coupled electron transfer mechanism. here we have studied the kinetics of both electron and proton transfer independently u ...201222536809
selection and application of endophytic bacterium achromobacter xylosoxidans strain f3b for improving phytoremediation of phenolic pollutants.while phytoremediation has been considered as an in situ bioprocess to remediate environmental contaminants, the application of functional endophytic bacteria within plants remains a potential strategy that could enhance the plants' efficiency in phytoremediation. in this study, 219 endophytes were isolated from plants that are predominantly located in a constructed wetland, including reed (phragmites australis) and water spinach (ipomoea aquatica). twenty-five strains of the isolated endophytes ...201222497718
a heat-stable cytotoxic factor produced by achromobacter xylosoxidans isolated from brazilian patients with cf is associated with in vitro increased proinflammatory cytokines.recently, achromobacter xylosoxidans has been related to chronic lung diseases in patients suffering from cystic fibrosis (cf), but its involvement has not been elucidated. some virulence properties of a. xylosoxidans isolated from brazilian patients with cf were revealed in this work.201222440828
characterization of a novel copper-haem c dissimilatory nitrite reductase from ralstonia pickettii.nirs (nitrite reductases) convert nitrite into no in the denitrification process. rpnir (ralstonia pickettii nir), a new type of dissimilatory cu-containing nir with a c-terminal haem c domain from r. pickettii, has been cloned, overexpressed in escherichia coli and purified to homogeneity. the enzyme has a subunit molecular mass of 50515 da, consistent with sequence data showing homology to the well-studied two-domain cu nirs, but with an attached c-terminal haem c domain. gel filtration and co ...201222414182
identification and distribution of achromobacter species in cystic fibrosis.we recently described a multilocus sequence typing scheme for achromobacter that identified several novel species in this genus.201323141756
alcaligenes xylosoxidans endocarditis of a prosthetic valve and pacemaker in a 62-year-old woman.the bacterium alcaligenes xylosoxidans is known to cause several nosocomial infections; however, it rarely causes endocarditis, which has a very high mortality rate. early isolation of the infection source and prompt identification of the patient's antibiotic sensitivities are paramount if the infection is to be treated adequately. we present what is apparently only the second documented case of the successful eradication of bioprosthetic valve endocarditis that was caused by pacemaker lead infe ...201323466992
respiratory bacterial infections in cystic fibrosis.bacterial respiratory infections are the main cause of morbidity and mortality in patients with cystic fibrosis (cf). pseudomonas aeruginosa remains the main pathogen in adults, but other gram-negative bacteria such as achromobacter xylosoxidans and stenotrophomonas maltophilia as well as nontuberculous mycobacteria have been shown to play an important role in the lung disease. the purpose of this review is to summarize the knowledge on disease and treatment of infection with cf-related pathogen ...201323449384
efficacy of disinfectants against naturally occurring and artificially cultivated bacteria.naturally occurring bacteria, is exist in nature, and is never cultivated on conventional culture medium. we evaluated the efficacy of disinfectants against naturally occurring bacteria in in-use cotton balls soaked in 0.02% benzalkonium chloride solution which had been used to disinfect the genital area by patients undergoing self-catheterization at home and the same bacteria subcultured on nutrient broth (artificially cultivated bacteria). the colony forming units (cfu) of naturally occurring ...201323449324
the effect of sinus surgery with intensive follow-up on pathogenic sinus bacteria in patients with cystic fibrosis.most patients with cystic fibrosis (cf) have chronic rhinosinusitis; their sinuses are often colonized with bacteria that can initiate and maintain deleterious pulmonary infections. theoretically, eradication of the sinus bacteria should reduce the frequency of lung infections and thereby reduce pulmonary morbidity. this article addressed whether bacteria in cf sinuses are eligible for eradication by sinus surgery and postoperative treatment.201323406585
picosecond binding of the his ligand to four-coordinate heme in cytochrome c': a one-way gate for releasing proximal no.we provide a direct demonstration of a "kinetic trap" mechanism in the proximal 5-coordinate heme-nitrosyl complex (5c-no) of cytochrome c' from alcaligenes xylosoxidans (axcp) in which picosecond rebinding of the endogenous his ligand following heme-no dissociation acts as a one-way gate for the release of proximal no into solution. this demonstration is based upon picosecond transient absorption changes following no photodissociation of the proximal 5c-no axcp complex. we have determined the a ...201323373628
innate aminoglycoside resistance of achromobacter xylosoxidans is due to axyxy-oprz, an rnd-type multidrug efflux pump.achromobacter xylosoxidans is an innately multidrug-resistant pathogen which is emerging in cystic fibrosis (cf) patients. we characterized a new resistance-nodulation-cell division (rnd)-type multidrug efflux pump, axyxy-oprz. this system is responsible for the intrinsic high-level resistance of a. xylosoxidans to aminoglycosides (tobramycin, amikacin, and gentamicin). furthermore, it can extrude cefepime, carbapenems, some fluoroquinolones, tetracyclines, and erythromycin. some of the axyxy-op ...201323089757
epidemiology and resistance of achromobacter xylosoxidans from cystic fibrosis patients in dijon, burgundy: first french data.achromobacter xylosoxidans is an emerging pathogen in cystic fibrosis (cf) patients recognised as causal agent of inflammation. the prevalence of infection or colonisation is variable among cf centres. we report here the first epidemiological data about a. xylosoxidans in a french cf centre: dijon, burgundy.201322944724
detection of achromobacter xylosoxidans in hospital, domestic, and outdoor environmental samples and comparison with human clinical isolates.achromobacter xylosoxidans is an aerobic nonfermentative gram-negative rod considered an important emerging pathogen among cystic fibrosis (cf) patients worldwide and among immunocompromised patients. this increased prevalence remains unexplained, and to date no environmental reservoir has been identified. the aim of this study was to identify potential reservoirs of a. xylosoxidans in hospital, domestic, and outdoor environments and to compare the isolates with clinical ones. from 2011 to 2012, ...201324038696
molecular characterization of achromobacter isolates from cystic fibrosis and non-cystic fibrosis patients in madrid, spain.multilocus sequence typing and nrda sequence analysis identified 6 different species or genogroups and 13 sequence types (sts) among 15 achromobacter isolates from cystic fibrosis (cf) patients and 7 species or genogroups and 11 sts among 11 isolates from non-cf patients. achromobacter xylosoxidans was the most frequently isolated species among cf patients.201323536401
complete genome sequence of the cystic fibrosis pathogen achromobacter xylosoxidans nh44784-1996 complies with important pathogenic phenotypes.achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. in this study we combined genomic analysis of a clinical isolated a. xylosoxidans strain with phenotypic investigations of its important pathogenic features. we present a complete assembly of the genome of a. xylosoxidans nh44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. the genome of a. xylosoxidans nh44784-1996 contains approximately 7 ...201323894309
nonfermenting gram-negative bacilli other than pseudomonas aeruginosa and acinetobacter spp. causing respiratory tract infections in a tertiary care center.nonfermenting gram-negative bacilli have emerged as important healthcare-associated pathogens. it is important to correctly identify all clinically significant nonfermenting gram-negative bacilli considering the intrinsic multidrug resistance exhibited by these bacteria.201324672175
cloning, expression and characterization of d-aminoacylase from achromobacter xylosoxidans subsp. denitrificans atcc 15173.d-aminoacylase catalyzes the conversion of n-acyl-d-amino acids to d-amino acids and fatty acids. the aim of this study was to identify the d-aminoacylase gene from achromobacter xylosoxidans subsp. denitrificans atcc 15173 and investigate the biochemical characterization of the enzyme. a previously uncharacterized d-aminoacylase gene (addan) from this organism was cloned and sequenced. the open reading frame (orf) of addan was 1467 bp in size encoding a 488-amino acid polypeptide. addan, with a ...201323369306
genome implosion elicits host-confinement in alcaligenaceae: evidence from the comparative genomics of tetrathiobacter kashmirensis, a pathogen in the making.this study elucidates the genomic basis of the evolution of pathogens alongside free-living organisms within the family alcaligenaceae of betaproteobacteria. towards that end, the complete genome sequence of the sulfur-chemolithoautotroph tetrathiobacter kashmirensis wt001(t) was determined and compared with the soil isolate achromobacter xylosoxidans a8 and the two pathogens bordetella bronchiseptica rb50 and taylorella equigenitalis mce9. all analyses comprehensively indicated that the rb50 an ...201323741407
characterization of pbt genes conferring increased pb2+ and cd2+ tolerance upon achromobacter xylosoxidans a8.the cluster of pbttfyrabc genes is carried by plasmid pa81. its elimination from achromobacter xylosoxidans a8 resulted in increased sensitivity towards pb(2+) and cd(2+). predicted pbttrabc products share strong similarities with pb(2+) uptake transporter pbrt, transcriptional regulator pbrr, metal efflux p1-atpases pbra and cada, undecaprenyl pyrophosphatase pbrb and its signal peptidase pbrc from cupriavidus metallidurans ch34. expression of pbtabc or pbta in a metal-sensitive escherichia col ...201324125695
gates and binding pockets for nitric oxide with cytochrome c', according to molecular dynamics.random-acceleration molecular-dynamics (ramd) simulations with models of homodimeric 6-ligated distal-no and 5-ligated proximal-no cytochrome c' complexes, in tip3 h2 o, showed two distinct, non-intercommunicating worlds. in the framework of a long cavity formed by four protein helices with heme at one extremity, no was observed to follow different pathways with the two complexes to reach the solvent. with the 6-ligated complex, no was observed to progress by exploiting protein internal channels ...201324078591
bacterial sinusitis can be a focus for initial lung colonisation and chronic lung infection in patients with cystic fibrosis.a major purpose of treating patients with cystic fibrosis (cf) is to prevent or delay chronic lung infections with cf-pathogenic gram-negative bacteria. in the intermittent stage, bacteria can usually be eradicated from the lungs with antibiotics, but following eradication, the next lung colonisations often occur with bacteria of identical genotype. this may be due to re-colonisation from the patient's paranasal sinuses. in our study, we found that approximately two-thirds of cf patients having ...201324064077
achromobacter xylosoxidans keratitis masquerading as recurrent erosion after lasik.to describe the clinical stages and management of achromobacter xylosoxidans keratitis with post-lasik epithelial ingrowth in two patients.201324063345
[bacteremia and pneumonia due to alcaligenes xylosoxidans in a immunocompetent patient]. 201323877784
perinephric abscess due to achromobacter xylosoxidans following de-roofing of renal cyst. 201323859686
distribution of allelic variants of the chromosomal gene bla oxa-114-like in achromobacter xylosoxidans clinical isolates.achromobacter xylosoxidans is increasingly being documented in cystic fibrosis patients. the bla(oxa-114) gene has been recognized as a naturally occurring chromosomal gene, exhibiting different allelic variants. in the population under study, the bla(oxa-114)-like gene was found in 19/19 non-epidemiological-related clinical isolates of a. xylosoxidans with ten different alleles including 1 novel oxa-114 variant.201323771548
achromobacter species in cystic fibrosis: cross-infection caused by indirect patient-to-patient contact.achromobacter species leads to chronic infection in an increasing number of cf patients. we report 2 cases of achromobacter ruhlandii cross-infection between patients after well-described indirect contact.201323769270
bacteremia due to achromobacter xylosoxidans in neonates: clinical features and outcome.we report an outbreak of achromobacter xylosoxidans at a neonatal intensive care unit. we aimed to present clinical, laboratory and treatment data of the patients.201323742802
early treatment with inhaled antibiotics postpones next occurrence of achromobacter in cystic fibrosis.in this nationwide retrospective study, we analysed species distribution, antimicrobial susceptibility and time to next occurrence of achromobacter in danish cystic fibrosis (cf) patients from 2000 to 2011.201323727271
evaluation of colistin susceptibility in multidrug-resistant clinical isolates from cystic fibrosis, france.the emergence of multidrug-resistant (mdr) bacteria in cystic fibrosis (cf) patients has led to the use of colistin drug and the emergence of colistin-resistant gram-negative bacteria. the aim of this study was to compare the disk diffusion and etest methods for colistin susceptibility testing on mdr bacteria associated with cf from marseille, france. forty-nine mdr clinical isolates (27 stenotrophomonas maltophilia, 22 achromobacter xylosoxidans) were used in this study. disk diffusion and etes ...201323719852
construction of a plant-microbe phytoremediation system: combination of vetiver grass with a functional endophytic bacterium, achromobacter xylosoxidans f3b, for aromatic pollutants removal.the endophytic bacterial strain achromobacter xylosoxidans f3b, which was able to utilize aromatic compounds as a sole carbon source, was inoculated into vetiver grass in this study. a real-time pcr detection method has been developed for confirming the stability of f3b in plants and dgge profiles were conducted for examining the diversity of endophytes during the remediation process. these results showed that the endophytic bacteria strain f3b could maintain a stable population in plant roots w ...201323591084
[bacterial isolates from respiratory samples of pediatric patients with cystic fibrosis and their distribution by ages].the bacterial isolates from respiratory samples of 50 pediatric patients with cystic fibrosis, their distribution by ages and antimicrobial resistance pattern as well as the intermittence of isolations and coinfections, were investigated. staphylococcus aureus was isolated in 72 % of patients, followed by pseudomonas aeruginosa (58 %), haemophilus. influenzae (56 %), and the burkholderia cepacia complex (12 %). the frequency of resistance of p. aeruginosa isolates to β-lactam antibiotics was low ...201323560788
bacterial strains isolated from pcb-contaminated sediments and their use for bioaugmentation strategy in microcosms.this study was focused on the characterization of 15 bacterial strains isolated from long-term pcb-contaminated sediment located at the strážsky canal in eastern part of slovakia, in the surroundings of a former pcb producer. pcb-degrading strains were isolated and identified as microbacterium oleivorans, stenotrophomonas maltophilia, brevibacterium sp., ochrobactrum anthropi, pseudomonas mandelii, rhodococcus sp., achromobacter xylosoxidans, stenotrophomonas sp., ochrobactrum sp., pseudomonas a ...201423553615
achromobacter xylosoxidans induced bronchiolitis obliterans in cystic fibrosis.we report a 12-year-old boy with progressive bronchiolitis obliterans caused by achromobacter xylosoxidans (ax) colonization after liver transplantation, resulting in a steep decline in lung function.201424039244
achromobacter xylosoxidans meningitis in an immunosuppressed patient. 201423970185
characterization of plasmid pax22, encoding vim-1 metallo-β-lactamase, reveals a new putative mechanism of in70 integron mobilization.vim-type enzymes are among the most widespread acquired metallo-β-lactamases among gram-negative pathogens. integron in70 is a class 1 integron that has emerged as a successful genetic support for blavim-1 (one of the most prevalent blavim allelic variants) in gram-negative non-fermenters, and is usually chromosome borne. the objective of this study was to characterize plasmid pax22 from achromobacter xylosoxidans ax22, which represents the only in70-harbouring plasmid known so far, to gather in ...201423928025
bioremediation assessment of diesel-biodiesel-contaminated soil using an alternative bioaugmentation strategy.this study investigated the effectiveness of successive bioaugmentation, conventional bioaugmentation, and biostimulation of biodegradation of b10 in soil. in addition, the structure of the soil microbial community was assessed by polymerase chain reaction-denaturing gradient gel electrophoresis. the consortium was inoculated on the initial and the 11th day of incubation for successive bioaugmentation and only on the initial day for bioaugmentation and conventional bioaugmentation. the experimen ...201424091525
imp-1 encoded by a novel tn402-like class 1 integron in clinical achromobacter xylosoxidans, china.achromobacter xylosoxidans strain a22732 is isolated from a pneumonia patient in china and produces carbapenemases oxa-114e and imp-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. the blaimp-1 gene together with aaca7 and orfe is captured by a novel tn402-like class 1 integron in a conjugative incp-1ß plasmid. in addition to the intrinsic integron promoter pcw, there is still a blaimp-1 gene cassette-spec ...201425428613
chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria.the study was conducted to isolate endosulfan biotransforming or biodegrading microbes based on chemotaxis. pseudomonas aeruginosa strain kkc3, ochrobactrum sp. strain kkc4, achromobacter xylosoxidans strain kkc6 and bacillus megaterium kkc7 were isolated based on their migration towards endosulfan in a soil column. out of the four bacteria, b. megaterium converted endosulfan into toxic metabolite endosulfan sulphate, while the other three bacteria followed the non-toxic endosulfan diol pathway. ...201425409584
interaction between atypical microorganisms and e. coli in catheter-associated urinary tract biofilms.most biofilms involved in catheter-associated urinary tract infections (cautis) are polymicrobial, with disease causing (eg escherichia coli) and atypical microorganisms (eg delftia tsuruhatensis) frequently inhabiting the same catheter. nevertheless, there is a lack of knowledge about the role of atypical microorganisms. here, single and dual-species biofilms consisting of e. coli and atypical bacteria (d. tsuruhatensis and achromobacter xylosoxidans), were evaluated. all species were good biof ...201425184430
achromobacter xylosoxidans in two haemodialysis patients. 201425036074
scleral buckle infection with alcaligenes xylosoxidans.we describe a rare case of extraocular inflammation secondary to scleral buckle infection with alcaligenes xylosoxidans. a 60-year-old female with a history of retinal detachment repair with open-book technique of scleral buckling presented with purulent discharge and irritation in the right eye that had begun 4 weeks earlier and had been treated ineffectively at another hospital. conjunctival erosion with exposure of the scleral buckle was noted. the scleral buckle was removed and cultured. the ...201425005211
pulmonary infection caused by achromobacter xylosoxidans in a patient with carcinoma of epiglottis: a rare case.achromobacter xylosoxidans is an aerobic, motile, oxidase and catalase positive, non-fermenting, gram negative bacillus. it is an opportunistic pathogen which is responsible for various nosocomial and community-acquired infections. however, there are only very few reports of pulmonary infections caused by this bacterium in cancer patients. we are presenting a case of a patient with carcinoma of epiglottis, who developed pulmonary infection caused by achromobacter xylosoxidans. according to the a ...201424995184
resolution of concomitant achromobacter xylosoxidans burn wound infection without adjustment of antimicrobial therapy.achromobacter xylosoxidans is part of an emerging group of gram negative bacterial infections with potentially severe sequelae, especially in the immunocompromised population such as burn patients. while antimicrobial therapy for patients with a. xylosoxidans bacteremia has been reported, the literature is scarce with regard to treatment in patients with positive tissue cultures only. herein, we report our institution's experience with such a case and a brief review of the current literature on ...201424987220
maldi-tof: a useful tool for laboratory identification of uncommon glucose non-fermenting gram-negative bacteria associated with cystic fibrosis.the predisposition of patients with cystic fibrosis (cf) for recurrent pulmonary infections can result in poor prognosis of the disease. although the clinical significance in cf of micro-organisms, such as staphylococcus aureus, haemophilus influenzae and pseudomonas aeruginosa, is well established, the implication of uncommon glucose non-fermenting gram-negative bacilli (ugnf-gnb) in respiratory samples from cf patients is still unclear. because of limitations of traditional methods used in mos ...201424980571
intracardiac abscess with cutaneous fistula secondary to ventricular septal defect repair simulating sternal wound infection.cutaneous fistula as a clinical presentation of intracardiac abscess of the right side is such an unusual occurrence that it has not until now been reported in the english-language medical literature. we present a rare case of right-sided infective endocarditis caused by achromobacter xylosoxidans in which recurrent infection presented as sternal wound discharge. the infection was found to have an intracardiac origin and was successfully managed by radical débridement on cardiopulmonary bypass.201424955054
fingerprinting redox and ligand states in haemprotein crystal structures using resonance raman spectroscopy.it is crucial to assign the correct redox and ligand states to crystal structures of proteins with an active redox centre to gain valid functional information and prevent the misinterpretation of structures. single-crystal spectroscopies, particularly when applied in situ at macromolecular crystallography beamlines, allow spectroscopic investigations of redox and ligand states and the identification of reaction intermediates in protein crystals during the collection of structural data. single-cr ...201424816098
altered bacterial profiles in saliva from adults with caries lesions: a case-cohort study.the aim of this study was to learn whether presence of caries in an adult population was associated with a salivary bacterial profile different from that of individuals without untreated caries. stimulated saliva samples from 621 participants of the danish health examination survey were analyzed using the human oral microbe identification microarray technology. samples from 174 individuals with dental caries and 447 from a control cohort were compared using frequency and levels of identified bac ...201424643218
putative p1b-type atpase from the bacterium achromobacter xylosoxidans a8 alters pb2+/zn2+/cd2+-resistance and accumulation in saccharomyces cerevisiae.pbta, a putative p(1b)-type atpase from the gram-negative soil bacterium achromobacter xylosoxidans a8 responsible for pb(2+)/zn(2+)/cd(2+)-resistance in escherichia coli, was heterologously expressed in saccharomyces cerevisiae. when present in zn(2+)- and pb(2+)/cd(2+)-hypersensitive s. cerevisiae strains cm137 and dty168, respectively, pbta was able to restore zn(2+)- and pb(2+)-resistant phenotype. at the same time, the increase of pb, zn, and cd accumulation in yeast was observed. however, ...201424491492
role of thiobacillus thioparus in the biodegradation of carbon disulfide in a biofilter packed with a recycled organic pelletized material.this study reports the biodegradation of carbon disulfide (cs2) in air biofilters packed with a pelletized mixture of composted manure and sawdust. experiments were carried out in two lab-scale (1.2 l) biofiltration units. biofilter b was seeded with activated sludge enriched previously on cs2-degrading biomass under batch conditions, while biofilter a was left as a negative inoculation control. this inoculum was characterized by an acidic ph and sulfate accumulation, and contained achromobacter ...201424469405
first genome sequences of achromobacter phages reveal new members of the n4 family.multi-resistant achromobacter xylosoxidans has been recognized as an emerging pathogen causing nosocomially acquired infections during the last years. phages as natural opponents could be an alternative to fight such infections. bacteriophages against this opportunistic pathogen were isolated in a recent study. this study shows a molecular analysis of two podoviruses and reveals first insights into the genomic structure of achromobacter phages so far.201424468270
l5-s1 achromobacter xylosoxidans infection secondary to oxygen-ozone therapy for the treatment of lumbosacral disc herniation: a case report and review of the literature.case report and literature review.201424384664
enhanced biodegradation of total polycyclic aromatic hydrocarbons (tpahs) by marine halotolerant achromobacter xylosoxidans using triton x-100 and β-cyclodextrin--a microcosm approach.ability of achromobacter xylosoxidans, a chrysene degrading marine halotolerant bacterium to degrade polycyclic aromatic hydrocarbons (pahs) using a cost effective laboratory microcosm approach, was investigated. effect of variables as chrysene, glucose as a co-substrate, triton x-100 as a non-ionic surfactant and β-cyclodextrin as a pahs solubilizer was examined on degradation of low molecular weight (lmw) and high molecular weight (hmw) pahs. a total of eleven pahs detected from polluted salin ...201424382467
prevalence of achromobacter xylosoxidans in pulmonary mucosa-associated lymphoid tissue lymphoma in different regions of europe.extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (malt lymphoma) comprises 7-8% of b-cell lymphomas and commonly originates from a background of long-standing chronic inflammation. an association with distinct bacteria species has been confirmed for several anatomical sites of malt lymphoma. for pulmonary malt lymphoma, however, a clear link with an infectious agent or autoimmune disorder has not yet been reported. using a 16s rrna gene-based approach, we have recently iden ...201424372375
skin and soft tissue infection caused by achromobacter xylosoxidans: report of 14 cases.skin and soft tissue infections (sstis) caused by achromobacter xylosoxidans are very infrequent. the aim of the present study was to investigate the clinical and microbiological characteristics of this infection.201424325336
the influence of bioaugmentation and biosurfactant addition on bioremediation efficiency of diesel-oil contaminated soil: feasibility during field studies.the study focused on assessing the influence of bioaugmentation and addition of rhamnolipids on diesel oil biodegradation efficiency during field studies. initial laboratory studies (measurement of emitted co2 and dehydrogenase activity) were carried out in order to select the consortium for bioaugmentation as well as to evaluate the most appropriate concentration of rhamnolipids. the selected consortium consisted of following bacterial taxa: aeromonas hydrophila, alcaligenes xylosoxidans, gordo ...201424291585
antibody response to achromobacter xylosoxidans during hiv infection is associated with lower cd4 levels and increased lymphocyte activation.inflammation during hiv infection is associated with worse disease outcomes and progression. many mechanisms have been indicted, including hiv itself, coinfections, and gut microbial translocation. concerning microbial translocation, we hypothesized that adaptive immune responses to a specific bacterial species known to be present in gut-associated lymphoid tissue are higher among hiv-infected individuals than among hiv-uninfected controls and are associated with t cell activation and lower cd4 ...201424173027
endophthalmitis caused by achromobacter xylosoxidans after cataract surgery.to report achromobacter xylosoxidans as a cause of both acute-onset and delayed-onset postoperative endophthalmitis after cataract surgery.201424150240
efficacy of surface disinfectant cleaners against emerging highly resistant gram-negative bacteria.worldwide, the emergence of multidrug-resistant gram-negative bacteria is a clinical problem. surface disinfectant cleaners (sdcs) that are effective against these bacteria are needed for use in high risk areas around patients and on multi-touch surfaces. we determined the efficacy of several sdcs against clinically relevant bacterial species with and without common types of multidrug resistance.201424885029
"watch out! pneumonia secondary to achromobacter denitrificans".pneumonia is the cause of significant morbidity and mortality especially in developing countries. the frequency and importance of emerging new pathogens have significant implications for therapy. we report a case of pneumonia caused by a very rare organism, achromobacter denitrificans which was treated successfully with intravenous meropenem injections for 14 days. review of available literature has documentation of isolation of achromobacter xylosoxidans from endotracheal aspirate culture but t ...201425031900
outbreak of achromobacter xylosoxidans in an italian cystic fibrosis center: genome variability, biofilm production, antibiotic resistance, and motility in isolated strains.cystic fibrosis (cf) patients have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. achromobacter xylosoxidans is a new emergent pathogen in cf spectrum. from 2005 to 2010 we had an outbreak in a. xylosoxidans prevalence in our cf center, thus, the present study was aimed at deeply investigating virulence traits of a. xylosoxidans strains isolated from infected cf patients. to this purpose, we assessed a. xylosoxidans genom ...201424772108
isolation and characterization of numerous novel phages targeting diverse strains of the ubiquitous and opportunistic pathogen achromobacter xylosoxidans.the clinical relevance of nosocomially acquired infections caused by multi-resistant achromobacter strains is rapidly increasing. here, a diverse set of 61 achromobacter xylosoxidans strains was characterized by multilocus sequence typing and phenotype microarray technology. the strains were further analyzed in regard to their susceptibility to 35 antibiotics and to 34 different and newly isolated bacteriophages from the environment. a large proportion of strains were resistant against numerous ...201424466294
poorly processed reusable surface disinfection tissue dispensers may be a source of infection.reusable surface disinfectant tissue dispensers are used in hospitals in many countries because they allow immediate access to pre-soaked tissues for targeted surface decontamination. on the other hand disinfectant solutions with some active ingredients may get contaminated and cause outbreaks. we determined the frequency of contaminated surface disinfectant solutions in reusable dispensers and the ability of isolates to multiply in different formulations.201424447780
phenotypic and genetic characterization of carbapenemase and esbls producing gram-negative bacteria (gnb) isolated from patients with cystic fibrosis (cf) in tehran hospitals.cystic fibrosis (cf) is an autosomal recessive genetic disorder in white populations caused by mutation in a gene that encodes cystic fibrosis transmembrane conductance regulator (cftr) protein. since frequent respiratory tract infections are the major problem in patients with cf, obligation to identify the causative bacteria and determining their antibiotic resistance pattern is crucial. the purpose of this project was to detect gram-negative bacteria (gnb) isolated from sputa of cf patients an ...201424596716
successful treatment with doripenem of ventriculitis due to achromobacter xylosoxidans. 201422411874
maldi-tof mass spectrometry: a powerful tool for clinical microbiology at hôpital principal de dakar, senegal (west africa).our team in europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms). to evaluate the utility of maldi-tof ms in tropical africa in collaboration with local teams, we installed an apparatus in the hôpital principal de dakar (senegal), performed routine identification of isolates, and confirmed or completed their identification in france. in the case of discordance or a la ...201526716681
comparative genomics of non-pseudomonal bacterial species colonising paediatric cystic fibrosis patients.the genetic disorder cystic fibrosis is a life-limiting condition affecting ∼70,000 people worldwide. targeted, early, treatment of the dominant infecting species, pseudomonas aeruginosa, has improved patient outcomes; however, there is concern that other species are now stepping in to take its place. in addition, the necessarily long-term antibiotic therapy received by these patients may be providing a suitable environment for the emergence of antibiotic resistance. to investigate these issues, ...201526401445
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