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achromobacter xylosoxidans n. sp. from human ear discharge. 19715316576
strains of achromobacter xylosoxidans from clinical material.eleven strains of achromobacter xylosoxidans have been received from among 1106 strains of gram-negative, non-fermentative bacteria submitted to the national collection of type cultures for computer-assisted identification since 1 january 1972. the strains showed resistance to a wide range of antimicrobial agents and five of the isolates possibly played a pathogenic role. the biochemical characteristics of these 11 strains were compared with those of three culture collection strains.1977886012
achromobacter xylosoxidans isolates in hawaii.clinical and bacteriological features of nine cases in which achromobacter xylosoxidans were isolated in hawaii are described. five cases were ear infections mixed with other gram-negative bacteria. colonial morphology, xylose oxidation, peritrichous flagella staining, and antibiotic susceptibility pattern assisted in separating this bacterium from other nonfermentative, oxidase-positive, gram-negative rods.1978632351
cerebral ventriculitis associated with achromobacter xylosoxidans.six patients in the neurosurgical ward of fukushima medical college hospital suffering from ventriculitis due to achromobacter xylosoxidans infection had undergone craniotomy or cranial trepanation before the infection. the strains of a. xylosoxidans isolated from the patients were resistant to streptomycin, ampicillin, cephaloridine, gentamicin, and colistin. they were also resistant to chlorhexidine digluconate (hibitane) in a concentration of 2%. when a study of the chlorhexidine used in the ...1978632360
[clinical and bacteriological studies on infections due to achromobacter xylosoxidans (author's transl)]. 1978660928
community-acquired bacteremic achromobacter xylosoxidans type iiia pneumonia in a patient with idiopathic igm deficiency.a man was hospitalized with bacteremic achromobacter xylosoxidans type iiia pneumonia. the authors are aware of no previously reported similar infections caused by this bacterium. a clinical cure was achieved with a combination of carbenicillin and kanamycin therapy. microtiter susceptibility testing revealed that carbenicillin was the antibiotic to which a. xylosoxidans iiia was most sensitive (minimal inhibitory concentration, 1.6 microgram/ml) and that synergy between carbenicillin and kanamy ...1978360830
semiquantitative catalase test as an aid in identification of oxidative and nonsaccharolytic gram-negative bacteria.a simple and rapid semiquantitative slide catalase test useful for the identification of oxidative and nonsaccharolytic gram-negative bacteria, i.e., "nonfermenters," is described. using the interpretative criterion of time of appearance of oxygen bubbles in 3% hydrogen peroxide, three categories of nonfermenters were established. the rapid catalase producers included achromobacter xylosoxidans and achromobacter species; acinetobacter anitratus and acinetobacter lwoffii; bordetella bronchiseptic ...1979393719
[an achromobacter xylosoxidans strain isolated from pleural empyema (author's transl)].the isolation of an achromobacter xylosoxidans strain from a 32 year old man with pleural empyema, and its biochemical characteristics are reported.1979554008
in vitro activity of cefoperazone against nonfermenters and aeromonas hydrophila.the in vitro activity of cefoperazone against 380 strains (33 species and unnamed groups) of nonferenters and 20 strains of aeromonas hydrophila was studied by a microdilution method of determining minimal inhibitory concentrations. for comparison, the activities of ampicillin, ticarcillin, and cefamandole were simultaneously studied. cefoperazone was the most active drug against pseudomonas sp., achromobacter xylosoxidans, flavobacterium meningosepticum. flavobacterium sp. (group iib), group iv ...19806448581
clinical and laboratory characteristics of achromobacter xylosoxidans infection.achromobacter xylosoxidans was isolated from six patients. the organism causes opportunistic infections in patients who are compromised. a. xylosoxidans is a catalase- and oxidase-positive, motile, gram-negative rod that oxidizes xylose and glucose. the organism exists in a water environment and may be confused with pseudomonas species. unlike pseudomonas, achromobacter has peritrichous flagella. the clinical and laboratory characteristics of a. xylosoxidans are presented.19807358838
[a case of suppurative skin disease which is thought to be due to achromobacter xylosoxidans (author's transl)]. 19806968842
prosthetic valve endocarditis due to achromobacter xylosoxidans. 19817211679
postoperative infection of an aortic prosthesis with achromobacter xylosoxidans. 19827064475
infective endocarditis caused by actinobacillus actinomycetemcomitans and achromobacter xylosoxidans. 19826957084
growth of non-campylobacter, oxidase-positive bacteria on selective campylobacter agar.a total of 67 oxidase-positive, gram-negative bacteria were tested for growth on selective campylobacter agar (blaser formulation, bbl microbiology systems, cockeysville, md.) at 42 degrees c under microaerophilic conditions. although the growth of most of these bacteria was prevented, all strains of achromobacter xylosoxidans, pseudomonas aeruginosa, pseudomonas putrefaciens, pseudomonas alcaligenes, and pseudomonas pseudoalcaligenes grew as well as campylobacter fetus subsp. jejuni.19826179961
experimental infection of immunocompromised mice with achromobacter xylosoxidans.the natural resistance of mice to achromobacter (a.) xylosoxidans was decreased to 1/80 by a single dose of 250 mg of cylophosphamide (cy) per kg intraperitoneally. when mice were infected with 1 x 10(9) a. xylosoxidans, the numbers of organisms in the liver, spleen, kidneys, lungs and heart blood reached to more than 10(6) per g at 6 hr after infection, and then decreased to less than 10(4) at 96 hr after infection. however, in the cy-treated mice, the numbers of organisms in these organs began ...19826803396
an outbreak of achromobacter xylosoxidans related to diagnostic tracer procedures.in december, 1978, an investigation was undertaken to determine the source of infection in five patients in one hospital with hospital-associated bacteremia due to achromobacter xylosoxidans. review of their records showed that each had a diagnostic tracer procedure preceding the bacteremia and that no other procedures were common to all. further investigation revealed that patients from three other hospitals were studied using diagnostic tracer materials from the index hospital. five patients w ...19826805314
the effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods.we studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, and acinetobacter calcoaceticus grew equally well at 25, 30, 37, and 42 c. among other sugar non-fermenting gram-negative rods, six species showed suppressed growth at either or both ends of the in ...19827087800
[chronological variations in blood isolates and their susceptibility to various antibiotics].chronological variation in blood isolates obtained from 1968 to 1982 was studied. escherichia coli was the most frequent isolates followed by klebsiella species, glucose-non-fermentative gram-negative rods (excluding pseudomonas aeruginosa), enterobacter-serratia group, and staphylococcus aureus. the isolation of glucose-non-fermentative gram-negative rods, about half of which being achromobacter xylosoxidans, increased greatly in number from 1980. a total of 113 strains of blood isolates was ex ...19836674551
examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy.we examined tenckhoff peritoneal catheters by scanning and transmission electron microscopy to study the morphology of bacterial adherence. two catheters were removed from uninfected patients, three from patients with exit site infections, four from patients with peritonitis, and one from a patient with both exit site infection and peritonitis. infecting organisms included three of staphylococcus aureus and one each of enterobacter sp., staphylococcus epidermidis, achromobacter xylosoxidans, ser ...19836228562
[pacemaker infection due to achromobacter xylosoxidans]. 19836415331
serological classification of achromobacter xylosoxidans.adult rabbits were immunized with nine achromobacter xylosoxidans strains by intravenous injection of formalin-killed organisms. antisera thus obtained were reciprocally titrated with the nine a. xylosoxidans strains, and seven sera were defined as serologically distinct. three of nine antisera possessed one common antibody while also each having their own specific antibody. ninety-five strains of a. xylosoxidans were examined for serotyping by a microtiter agglutination test with the nine antis ...19836833473
a comparative study of avian bordetella-like strains, bordetella bronchiseptica, alcaligenes faecalis and other related nonfermentable bacteria.cultural and biochemical characteristics, electrophoretic patterns of soluble proteins, and pathogenicity in turkey poults, of 30 avian gram-negative nonfermentable bacterial strains acquired from various geographical regions, of which 26 had been isolated from turkeys and one each from a chicken, duck, goose and sharp-tailed munia, were compared to those of bordetella bronchiseptica, alcaligenes faecalis, alcaligenes denitrificans, achromobacter xylosoxidans and bordetella parapertussis. with t ...198318766782
nosocomial colonization and infection by achromobacter xylosoxidans.achromobacter xylosoxidans, a bacterial species named in 1971, is often isolated from aqueous environments, but little has been reported about its pathogenicity in humans, its epidemiological pattern, and its susceptibility to antibiotics and antiseptics. we were faced with an epidemic caused by this microorganism for 18 months in an intensive care unit. two patients had fatal infections and 37 others were colonized. the source was the deionized water of the hemodialysis system. the 46 isolates ...19846699141
corneal ulcer due to achromobacter xylosoxidans.we report a case of corneal ulcer caused by the opportunistic organism achromobacter xylosoxidans which developed during chronic topical steroid treatment of an eye with neovascular glaucoma. a. xylosoxidans has probably been underreported as a cause of ocular infection because of confusion between this organism and other gram-negative organisms, particularly pseudomonas. a. xylosoxidans is resistant to aminoglycosides and some cephalosporins but not carbenicillin. this difference in antibiotic ...19846733072
evaluation of the rapid nft system for identification of gram-negative, nonfermenting rods.this study evaluated the ability of the rapid nft system (api system sa, montalieu-vercieu, france) to accurately identify 262 clinically isolated, gram-negative, nonfermentative rods without additional tests. identifications were classified as correct; low discrimination, with a spectrum of two or more possibilities (additional tests necessary for accurate identification); and incorrect. correct identification rates were analyzed in two categories: (i) correct to species or biotype for all orga ...19846490857
occurrence and antimicrobial susceptibility of gram-negative nonfermentative bacilli in cystic fibrosis patients.isolation of nonfermentative gram-negative bacilli (other than pseudomonas aeruginosa) from respiratory tract cultures of cystic fibrosis (cf) patients has increased in recent years. species recovered include pseudomonas cepacia, p. maltophilia, p. fluorescens/putida, p. alcaligenes, p. pseudoalcaligenes, p. stutzeri, acinetobacter spp., achromobacter xylosoxidans, flavobacterium spp., and cdc groups ive and ve. although colonization with most of these organisms is sporadic, p. cepacia (and to a ...19853979021
antibiotic susceptibilities of achromobacter xylosoxidans. 19853834609
neonatal meningitis caused by achromobacter xylosoxidans.the clinical and bacteriological findings in a case of neonatal meningitis caused by achromobacter xylosoxidans are presented. this appears to be only the second report of meningitis caused by this species.19854044804
infections with achromobacter xylosoxidans. 19863715491
[amino acid metabolism by thin layer chromatography for the differentiation of bordetella and alcaligenes species].the amino acid metabolism of bordetella and alcaligenes strains was investigated for differentiation purposes. all species investigated reacted differently with a number of amino acids. the genus bordetella could be differentiated from that of alcaligenes by its inability to metabolize l-lysine, l-arginine and d-histidine. the recently described species bordetella avium, as well as achromobacter xylosoxidans (yabuuchi), considered as alcaligenes denitrificans subsp. xylosoxidans, presented thems ...19863788350
infections due to achromobacter xylosoxidans. case report and review of the literature.achromobacter xylosoxidans is an uncommon nosocomial pathogen known to cause many serious infections. a 69-year-old woman with diabetes mellitus and chronic renal failure was admitted with pulmonary edema. the patient developed fever and pulmonary infiltrate with bilateral pleural effusions while she was on a respirator in the intensive care unit. culture of sputum, pleural fluid and blood grew a. xylosoxidans. bilateral chest tubes were inserted and the patient was treated for one month with pi ...19863818105
purification and some properties of cytochrome c' from a strain of achromobacter xylosoxidans.cytochrome c' was crystallized from achromobacter xylosoxidans gifu 543. the cytochrome was a basic protein and its molecular weight was 28,000. the pyridine ferrohemochrome showed absorption peaks at 415, 521, and 551 nm. the absorption spectra of the oxidized and reduced forms at neutral ph were almost the same as those of other cytochromes c' reported already. the reduced cytochrome c' reacted with co and no, and the no complex showed a characteristic absorption spectrum. the midpoint redox p ...19863017925
in-vitro activity of newer quinolones against aerobic bacteria.nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ...19862940214
peritonitis caused by alcaligenes denitrificans subsp. xylosoxydans: case report and review of the literature.we report the third human case of peritonitis caused by alcaligenes denitrificans subsp. xylosoxydans and review the english literature regarding community-acquired and nonsocomial infection and colonization that results from this bacterium. the biochemical and genetic characteristics supporting the pathogenic potential of a. denitrificans subsp. xylosoxydans are reviewed, and the antimicrobial susceptibility profile of the organism is summarized.19863771775
plasmid pcbi carries genes for anaerobic benzoate catabolism in alcaligenes xylosoxidans subsp. denitrificans pn-1.pseudomonas sp. strain pn-1 is reclassified as alcaligenes xylosoxidans subsp. denitrificans pn-1. strain pn-1 is a gram-negative, rod-shaped organism, is motile by means of lateral flagella, is oxidase positive, and does not ferment sugars. plasmid pcbi, carrying genes for the anaerobic degradation of benzoate in strain pn-1, is 17.4 kilobase pairs in length and is transmissible to a number of denitrifying pseudomonas aeruginosa and pseudomonas stutzeri strains. a restriction endonuclease map w ...19872822651
emerging microorganisms in cystic fibrosis.pseudomonas aeruginosa is the most common bacterial isolate obtained from patients with cystic fibrosis of the lungs. recently, however, new multiresistant organisms have emerged, whose identification may be difficult and whose pathogenic role proves hard to define. of the 71 strains isolated from 24 patients with cystic fibrosis during acute flareups of pulmonary symptoms, 48 turned out to be pseudomonas aeruginosa (67.6%); 11 were pseudomonas non-aeruginosa (15.5%); and 12 were achromobacter x ...19873103930
achromobacter xylosoxidans bacteremia. 19873595393
achromobacter xylosoxidans meningitis: case report and review of the literature. 19873621776
achromobacter xylosoxidans bacteremia.achromobacter xylosoxidans is a rare cause of bacteremia. a case of community-acquired pneumonia and bacteremia due to achromobacter in a patient with concomitant pulmonary tuberculosis is reported herein. the majority of patients who have developed achromobacter bacteremia have had a predisposition to infection (although the predisposing conditions have been diverse). immunosuppression has been reported in only one of the seven patients with achromobacter bacteremia for whom detailed informatio ...19873317727
immunological properties of a penicillinase produced by alcaligenes denitrificans subsp. xylosoxydans.rabbit antiserum was prepared to the penicillinase of alcaligenes denitrificans subsp. xylosoxydans gn14062. the antibody gave a single precipitin line in double immunodiffusion assays with the penicillinases from strain gn14062 and from two other a. denitrificans subsp. xylosoxydans strains. the precipitin line given by the gn14062 enzyme gave a reaction of complete identity with the precipitin lines of the penicillinases from the other a. denitrificans subsp. xylosoxydans strains. furthermore, ...19873119550
diversity of plasmids in achromobacter xylosoxidans isolates responsible for a seemingly common-source nosocomial outbreak.achromobacter xylosoxidans, an uncommon yet highly resistant opportunistic pathogen, was isolated from nine hospitalized patients during an 8-month period. it had been isolated from only seven patients with either nonfatal infection or colonization from 1981 to 1984. from june 1985 to january 1986, a. xylosoxidans was isolated 18 times from seven different sites (sputum, 7 times; urine, 4 times; blood, 3 times; and lung, pleural fluid, wound tissue, and tracheal aspirate, 1 time each). four pati ...19873667915
nitric oxide complex of cytochrome c' in cells of denitrifying bacteria.electron paramagnetic resonance (epr) spectra at 77k have been measured for the dried cells of three groups of chemoheterotrophic bacteria cultured in the presence of nitrate: i, denitrifying bacteria containing cytochrome c' (alcaligenes sp. ncib 11015, achromobacter xylosoxidans gifu 543); ii, denitrifying bacteria in which cytochrome c' has not been found (alcaligenes faecalis iam 1015, bacillus firmus nias 237, pseudomonas stutzeri atcc 17641); iii, non-denitrifying bacterium containing cyto ...19882844743
osteomyelitis secondary to trauma or infected contiguous soft tissue.nonhematogenous osteomyelitis (nho) occurred in 24 pediatric patients (ages 8 months to 18 years; median, 14 years; 23 male) admitted from 1980 to 1985. predisposing factors included compound fracture (12), deep decubiti (4) and foot puncture (3). infection involved tibia (7), foot bones (6), proximal femur (3) and ulna (2). patients presented with drainage (64%), pain or tenderness (44%) and fever (32%) lasting for 1 to 180 days (median, 10 days). in 24% both white blood cell count and erythroc ...19882893333
outbreak of infection with achromobacter xylosoxidans from contaminated intravascular pressure transducers.achromobacter xylosoxidans contaminating transducers caused 15 cases of hospital infection. in the eight patients with bacteraemia the interval from inoculation to fever was an average of 6.6 days. all the infected patients recovered. computerization of laboratory records allowed retrieval of previous isolates, and review of clinical records focused the problem on patients with cardiac and aortic diseases. the problem arose from the re-use of disposable equipment after disinfection with a benzal ...19882905368
studies on ampicillin resistance in achromobacter xylosoxidans. brief report.population analyses of susceptibility to ampicillin in ampicillin-susceptible and ampicillin-resistant strains of achromobacter xylosoxidans revealed the existence of ampicillin-resistant subpopulations in ampicillin-susceptible isolates. bacteria resistant to a concentration four times the one that inhibited the majority population had a frequency of 10(-3) to 10(-4). strains isolated from aqueous environments are often found susceptible to ampicillin, while sporadic cases of infections with a. ...19883345263
achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) bacteremia associated with a well-water source: case report and review of the literature.a case of community-acquired achromobacter xylosoxidans bacteremia in a patient with metastatic breast carcinoma is described. the patient's home drinking water was identified as the source of her bacteremia. the case represents the first in which a community-acquired infection due to this organism has been attributed to a documented water source.19883281982
evaluation of 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) in broth and agar media for identification of pseudomonas aeruginosa.brain heart infusion broth and mueller-hinton agar containing the compound 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) were evaluated as selective media for identifying pseudomonas aeruginosa. of the 192 pseudomonas spp. and 68 additional oxidase-positive or glucose-nonfermenting gram-negative organisms tested, only p. aeruginosa (120 of 121 isolates) and certain strains of pseudomonas cepacia, aeromonas hydrophila, and achromobacter xylosoxidans wer ...19883141473
nosocomial outbreak of achromobacter xylosoxidans associated with a diagnostic contrast solution. 19883170779
achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) meningitis associated with a gunshot wound.the microbiological and clinical features of a case of achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) meningitis associated with a gunshot wound are described. to our knowledge, this is the third confirmed case report of meningitis caused by this organism.19883235669
nosocomial achromobacter xylosoxidans infections.a xylosoxidans is being recognized as an important nosocomial pathogen. as more patients are rendered immunosuppressed by chemotherapy, this organism's increasing role in hospital-acquired infections will be assured. achromobacter is a water-borne organism, highly resistant to most antibiotics, and even to some disinfectant solutions, and easily establishes itself in the hospital aquatic environment. achromobacter infections and outbreaks should be recognized and approached as serious problems r ...19883278052
production of d-aminoacylase from alcaligenes denitrificans subsp. xylosoxydans mi-4.a bacterial strain that produces d-aminoacylase was isolated from soil and identified as alcaligenes denitrificans subsp. xylosoxydans mi-4. l-aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-aminoacylase was inducibly produced. n-acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. enzymatic resolution of n-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was appr ...198816347776
application of gas-liquid chromatography to the routine identification of nonfermenting gram-negative bacteria in clinical specimens.a total of 430 strains of glucose-nonfermenting gram-negative bacteria representing 35 species were analyzed for their cellular fatty acid composition by gas-liquid chromatography (glc). on the basis of qualitative differences in their cellular fatty acid composition, these bacteria could be divided into 19 distinct chromatographic groups. eight pseudomonas species, achromobacter xylosoxidans, group vd, and agrobacterium radiobacter were identified from their fatty acid compositions alone. the o ...19892768442
[bacterial infections of the ear: case contribution on clinical and microbiological aspects].ciprofloxacin was given in oral doses of 250 mg. each 8 hours to 21 patients with otitis. the efficacy of treatment was assessed by clinical and bacteriological parameters. the 61.9% of isolated bacteria are opportunistic. a case of chronic otitis by achromobacter xylosoxidans is described.19892490403
fatal neonatal meningitis and ventriculitis caused by multiresistant achromobacter xylosoxidans. a case report.meningitis and ventriculitis in a 6-day-old neonate caused by a gram-negative glucose-non-fermenting organism, achromobacter xylosoxidans, was resistant to most antibiotics except ceftazidime and imipenem. the organism became resistant after 28 days treatment with ceftazidime. when the infant was 7 weeks old, imipenem became available but, in spite of 3 days of intravenous treatment, the organism was still recovered from ventricular cerebrospinal fluid and the child died. this would appear to be ...19892588089
achromobacter xylosoxidans corneal ulcer in a therapeutic soft contact lens wearer.achromobacter xylosoxidans is an opportunistic organism that is usually seen in immunocompromised or immunosuppressed patients. it is an aerobic gram-negative rod, often confused with other more commonly seen gram-negative bacteria such as pseudomonas aeruginosa. the organism is usually sensitive to extended spectrum penicillins such as carbenicillin and usually resistant to aminoglycosides and first generation cephalosporins. we wish to describe a corneal ulcer from a. xylosoxidans that develop ...19892805715
epr spectra of ferric cytochromes c' from five strains of achromobacter xylosoxidans at low temperature and their temperature dependence.the epr spectra at low temperature (6 k) and their temperature dependence (10-93 k) for five ferric cytochromes c' isolated from chemoheterotrophic bacteria, achromobacter xylosoxidans ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, gifu 1048, gifu 1051, and gifu 1764 are reported. the epr spectral results indicate that the ground state of the heme iron(iii) of cytochromes c' from these chemoheterotrophic bacteria can appear to be in an admixed spin state which consists of predominan ...19902163634
susceptibility of alcaligenes denitrificans subspecies xylosoxydans to beta-lactam antibiotics.the susceptibility of 56 clinical isolates and two reference strains of alcaligenes denitrificans subsp. xylosoxydans to beta-lactam agents was tested and related to beta-lactamase activity of the strains. the mics of 12 beta-lactams determined by an agar dilution method showed that all the strains were sensitive to imipenem and moxalactam. forty-one cloxacillin-sensitive beta-lactamase producing strains were highly susceptible to azlocillin, piperacillin and ticarcillin, and less susceptible to ...19902387293
two resistance phenotypes to beta-lactams of alcaligenes denitrificans subsp. xylosoxydans in relation to beta-lactamase types. 19902351630
catheter-related right-sided endocarditis in bone marrow transplant recipients.bone marrow transplant recipients are at increased risk of severe central venous catheter-related septicemias that may be complicated by endocardial infection. in view of this, we prospectively evaluated 141 consecutive patients receiving allogeneic or autologous bone marrow infusion. seven (5%) of 141 patients developed eight episodes of a clinical syndrome compatible with catheter-related right-sided infective endocarditis; this diagnosis was confirmed at autopsy in two patients who died. stap ...19902330480
achromobacter xylosoxidans: a drug-resistant pathogen in newborns and immunocompromised patients. 19902237562
absence of role for plasmids in resistance to multiple disinfectants in three strains of bacteria.three chlorhexidine-resistant strains, two of achromobacter xylosoxidans, one of pseudomonas cepacia, were isolated from clinical sources. the three strains showed cross-resistance to benzethonium chloride, benzalkonium chloride and alkyldiaminoethylglycine. resistance persisted after treatment of the strains with acridine orange (12.5 mg-25 mg l-1). conjugation to appropriate recipients of escherichia coli was unsuccessful and plasmid dna could not be detected. these results suggest that plasmi ...19901969437
localization of the cytochrome cd1 and copper nitrite reductases in denitrifying bacteria.the locations of cytochrome cd1 nitrite reductases in pseudomonas aeruginosa and pseudomonas fluorescens and copper nitrite reductases in achromobacter cycloclastes and achromobacter xylosoxidans were identified. immunogold labeling with colloidal-gold probes showed that the nitrite reductases were synthesized exclusively in anaerobically grown (denitrifying) cells. little immunogold label occurred in the cytoplasm of these four strains; most was found in the periplasmic space or was associated ...19902158973
evaluation of autoscan-w/a automated microbiology system for the identification of non-glucose-fermenting gram-negative bacilli.we evaluated the ability of the autoscan-w/a (microscan division, baxter healthcare corporation, west sacramento, calif.), in conjunction with the dried colorimetric neg id type 2 panel (dcp) and new rapid fluorometric neg id panel (rfp), to identify non-glucose-fermenting gram-negative bacilli by challenging the system with 310 previously identified reference strains. of these 310 isolates, 286 organisms were in the dcp data base and 269 were in the rfp data base. use of the dcp panels resulted ...19902199522
in vitro activity of pd127,391, a new quinolone against bacterial isolates from cancer patients.the in vitro activity of pd127,391, a new 4-quinolone, was compared to that of ciprofloxacin against common clinical bacterial isolates from patients with cancer. pd127,391 was found to have a broad antimicrobial spectrum with excellent activity against gram-positive isolates (including multidrug-resistant organism such as corynebacterium jeikeium, enterococcus faecalis, enterococcus faecium, methicillin-resistant staphylococcus aureus and coagulase-negative staphylococcus spp.). it was also ext ...19902209169
achromobacter xylosoxidans bacteremia. 19902230043
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates].this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ...19902287060
[susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ...19911798067
[an uncommon germ of meningitis, achromobacter xylosoxidans]. 19911829828
susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to newer antimicrobials.the susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to a variety of newer antimicrobials was determined. sixty-three amikacin-resistant isolates were collected from 61 inpatients at a tertiary-care hospital between 1985 and 1988. the organisms included xanthomonas maltophilia (24 isolates), pseudomonas aeruginosa (16), pseudomonas species (3), serratia marcescens (10), flavobacterium meningosepticum (4), enterobacter species (3), acinetobacter calcoaceticu ...19911860303
novel cyanide-hydrolyzing enzyme from alcaligenes xylosoxidans subsp. denitrificans.a cyanide-metabolizing bacterium, strain df3, isolated from soil was identified as alcaligenes xylosoxidans subsp. denitrificans. whole cells and cell extracts of strain df3 catalyzed hydrolysis of cyanide to formate and ammonia (hcn + 2h2o----hcooh + nh3) without forming formamide as a free intermediate. the cyanide-hydrolyzing activity was inducibly produced in cells during growth in cyanide-containing media. cyanate (ocn-) and a wide range of aliphatic and aromatic nitriles were not hydrolyze ...19911872607
purification and some properties of phospholipase c from achromobacter xylosoxidans.a non-haemolytic phospholipase c (ec 3.1.4.3) was purified from the culture medium of achromobacter xylosoxidans with a 5% yield and a purification factor of 330. a combination of ultrafiltration, acetone precipitation and two subsequent affinity chromatographic steps was used. the affinity chromatography is a new application of 2-(4-aminophenylsulphonyl)ethyl-cellulose, a sorbent that has previously been used for the purification of phospholipase c from bacillus cereus. the purified enzyme gave ...19911783637
puncture wound-induced achromobacter xylosoxidans osteomyelitis of the foot. 19911674638
achromobacter xylosoxidans infection in baboons. 19911666159
[structure and function of cytochrome c' from achromobacter xylosoxidans]. 19911665239
spectral properties of achromobacter xylosoxidans cytochromes c' and their no complexes.cytochromes c' have been isolated from six strains of achromobacter xylosoxidans: ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, 1048, 1051, 1055 and 1764. they are dimeric proteins with more positive redox potentials than those of cytochromes c' from phototrophic bacteria at neutral ph. the electronic absorption, epr and mcd spectra on no-ferrous cytochromes c' at physiological ph showed that the major part of the heme-iron of nitrosylheme was penta-coordinated. the epr spectral re ...19911646026
achromobacter xylosoxidans septic arthritis in a patient with systemic lupus erythematosus. 19911953827
in vitro antibacterial activity of me1207, a new oral cephalosporin.me1207 is the prodrug of me1206. its in vitro antibacterial activity was compared with that of cefteram, cefpodoxime, cefixime, and cefaclor against various clinical isolates. me1206 was more active than the other cephems tested against staphylococci, streptococci, morganella morganii, pseudomonas cepacia, and flavobacterium meningosepticum and had the most potent activity against haemophilus influenzae and neiserria gonorrhoeae. the drug also showed a wide spectrum of activity against other gra ...19911929344
purification and characterization of n-acyl-d-glutamate deacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6.the purification and properties of n-acyl-d-glutamate deacylase from the cell extracts of alcaligenes xylosoxydans subsp. xylosoxydans a-6 were studied. the two active fractions (peaks i and ii) were obtained by a mono q column chromatography. the predominant enzyme (peak i) has been purified, 1960-fold to homogeneity and characterized. the enzyme was a monomer with a molecular weight of 59,000. the optimum ph and the isoelectric point were 8.0 and 5.5, respectively. the enzyme catalyzed the hyd ...19911894006
[achromobacter xylosoxidans septicemia]. 19911827195
[sepsis caused by achromobacter xylosoxidans]. 19911863629
achromobacter xylosoxidans. an unusual neonatal pathogen.perinatal acquisition of a rare pediatric pathogen, achromobacter xylosoxidans, with evidence for in utero transmission, is described. cultures from the mother and neonate demonstrated a. xylosoxidans. an ascending bacterial infection in the mother with clinical chorioamnionitis is presented as the probable mode of transmission. postmortem examination of the infant confirmed achromobacter meningitis. in contrast to the current case with transmission from mother to neonate, previously published n ...19911862776
high-level nickel resistance in alcaligenes xylosoxydans 31a and alcaligenes eutrophus kto2.two new nickel-resistant strains of alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. alcaligenes xylosoxydans 31a is a heterotrophic bacterium, and alcaligenes eutrophus kto2 is an autotrophic aerobic hydrogen-oxidizing bacterium. both strains carry-among other plasmids-a megaplasmid determining resistance to 20 to 50 mm nicl(2) and 20 mm cocl(2) (when growing ...199116348590
identification of xenobiotic-degrading isolates from the beta subclass of the proteobacteria by a polyphasic approach including 16s rrna partial sequencing.nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. the presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone q-8) allowed allocation of these organisms to the beta subclass of the proteobacteria. on the basis of the results of additional characterization experiments (i.e., api 20ne tests, determinations of soluble protein patterns, and dna-dn ...19921371062
bacterial arthritis.the 1991 literature on septic arthritis included a concise review of adult septic arthritis, examples of pseudoseptic arthritis, and two interesting animal studies. one animal study examined the induction of acute synovitis by the intra-articular injection of bacterial endotoxin and the cytokines tumor necrosis factor-alpha, and interleukin-1 beta; and the other studied the effects of early and delayed synovectomy in the management of septic arthritis. the predispositions to septic arthritis can ...19921503874
effect of storage time with different lens care systems on in-office hydrogel trial lens disinfection efficacy: a multi-center study.a combined prospective and retrospective study was conducted to evaluate the efficacy of in-office disinfection methods for hydrogel trial contact lenses. two hundred and twenty-one trial contact lenses, disinfected by four different disinfection methods, were collected from seven study centers and cultured for microbial contamination after various storage periods. negative and positive control lenses were included as an additional center in this double-masked study. there was a significant diff ...19921437007
prosthetic knee infection due to achromobacter xylosoxidans.achromobacter xylosoxidans is an aerobic gram negative organism that has been infrequently implicated in clinical infections in a variety of anatomical sites. we describe a case of a prosthetic knee infection due to achromobacter xylosoxidans in a patient with rheumatoid arthritis receiving high dose prednisone.19921404142
catheter-associated sepsis caused by ochrobactrum anthropi: report of a case and review of related nonfermentative bacteria.ochrobactrum anthropi, formerly known as cdc group vd, is an oxidase-producing, gram-negative, non-lactose-fermenting bacillus that oxidizes glucose and grows readily on macconkey agar. only occasionally isolated from human clinical specimens, this organism has rarely been found to be pathogenic. we describe the first reported case of infection due to o. anthropi in a child, that of bacteremia in a 3-year-old girl undergoing chemotherapy for retinoblastoma. in addition, we review the literature ...19921576286
bacteremia due to achromobacter xylosoxidans in patients with cancer.bacteremia due to achromobacter xylosoxidans is rare, and little information on treatment is available. between 1983 and 1988, a. xylosoxidans was recovered from 26 cultures of blood from 10 patients with cancer and clinical signs of infection, including one patient with septic shock and two with pneumonia. neutropenia did not seem to be a predisposing factor. the infection may have been catheter related in four patients and associated with gastrointestinal pathology in four others. probable cau ...19921554834
molecular cloning and characterization of catechol 2,3-dioxygenases from biphenyl/polychlorinated biphenyls-degrading bacteria.catechol 2,3-dioxygenases were cloned from alcaligenes sp. kf711, pseudomonas putida kf715, and achromobacter xylosoxidans kf701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. all of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (page). the purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. the catechol 2,3-dioxygenase from alcaligenes sp. kf711 exhibit ...19921530619
characterization of catechol 2,3-dioxygenases.three catechol 2,3-dioxygenases for biphenyl, naphthalene/salicylate, and toluene/xylene oxidation were cloned from achromobacter xylosoxidans kf701, pseudomonas putida (nah7), and pseudomonas sp. (pwwo). the cloned catechol 2,3-dioxygenases were identified by enzymatic activity assay in addition to yellow bands on polyacrylamide gel after electrophoresis and activity staining. all of the cloned catechol 2,3-dioxygenases exhibited their highest activities on catechol as a substrate compared with ...19921543511
evaluation of the 4-hour rapid nf plus method for identification of 345 gram-negative nonfermentative rods.the ability of the rapid nf plus system (innovative diagnostic systems, inc., atlanta, ga.) to identify 345 nonfermentative gram-negative rods was evaluated. kits were inoculated with no. 1 mcfarland suspensions, and reactions were interpreted after a 4-h incubation at 35 degrees c. overall, the method correctly identified 311 strains (90.1%) without additional tests and 21 strains (6.1%) with additional tests, and 13 strains (3.8%) were misidentified. five of 13 misidentified strains were alcal ...19921583129
a beta-lactamase-overproducing strain of alcaligenes denitrificans subsp. xylosoxydans isolated from a case of meningitis.a resistant (r) clinical isolate of alcaligenes denitrificans subsp. xylosoxydans was recovered from csf during treatment including piperacillin. this variant selected in vivo, and a second variant obtained in vitro from the initially susceptible (s) strain, both exhibited resistance to penicillins (ticarcillin, piperacillin) and cephalosporins, but remained susceptible to latamoxef and imipenem. clavulanate (2 mg/l) restored the susceptibility of the two r-variants to penicillins. a beta-lactam ...19921289351
opportunistic nosocomial multiply resistant bacterial infections--their treatment and prevention.one of the most difficult problems confronting the clinician who deals with nosocomial infections is that of microbial resistance. the predominant nosocomial infections (urinary tract infections, pneumonia, septicaemia, surgical wound infections) involve increasing numbers of gram-positive bacteria, staphylococcus epidermidis, corynebacterium jeikeium or resistant enterococci as well as new multiresistant gram-negative bacilli such as xanthomonas maltophilia, acinetobacter baumannii and alcalige ...19937755661
phylogenetic position of taylorella equigenitalis determined by analysis of amplified 16s ribosomal dna sequences.the 16s ribosomal dna sequence of taylorella equigenitalis (formerly haemophilus equigenitalis), the causative organism of contagious equine metritis, was determined. a phylogenetic analysis of this sequence revealed a phylogenetic position of t. equigenitalis in the beta subclass of the class proteobacteria apart from the position of haemophilus influenzae, which belongs to the gamma subclass of proteobacteria. a close phylogenetic relationship among t. equigenitalis, alcaligenes xylosoxidans, ...19938347520
nosocomial neonatal meningitis by alcaligenes xylosoxidans transmitted by aqueous eosin. 19937692379
purification and characterization of novel n-acyl-d-aspartate amidohydrolase from alcaligenes xylosoxydans subsp. xylosoxydans a-6.alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) produced n-acyl-d-aspartate amidohydrolase (d-aaase) in the presence of n-acetyl-d-aspartate as an inducer. the enzyme was purified to homogeneity. the enzyme had a molecular mass of 56 kda and was shown by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page) to be a monomer. the isoelectric point was 4.8. the enzyme had maximal activity at ph 7.5 to 8.0 and 50 degrees c, and was stable at ph 8.0 and up to 45 de ...19937763985
production, purification, and characterization of d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6.the best inducers for d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) were a poor substrate, n-acetyl-gamma-methyl-d-leucine, and an inhibitor, n-acetyl-d-alloisoleucine. the enzyme has been homogeneously purified. the molecular weight of the native enzyme was estimated to be 58,000 by gel filtration. a subunit molecular weight of 52,000 was measured by sds-page, indicating that the native protein is a monomer. the isoelectric point was 5.2. the enzyme was ...19937763986
pulmonary abscess associated with alcaligenes xylosoxidans in a patient with aids. 19938110940
controlled clinical comparison of two lysis-based blood culture systems, isolator and septi-chek release, for detection of bloodstream infections.a controlled clinical comparison was made of the isolator (wampole laboratories, cranbury, n.j.) and the septi-chek release bottle (roche diagnostics, nutley, n.j.). from 6,345 blood culture sets fulfilling minimum criteria for volume of blood cultured, 840 strains were isolated, of which only 691 (82%) were considered to be representative of bloodstream infection according to centers for disease control definitions. statistically significant differences were found between the systems for the fo ...19938370739
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