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denitrification by cystic fibrosis pathogens - stenotrophomonas maltophilia is dormant in sputum.chronic pseudomonas aeruginosa lung infection is the most severe complication for cystic fibrosis (cf) patients. infected endobronchial mucus of cf patients contains anaerobic zones mainly due to the respiratory burst of polymorphonuclear leukocytes. we have recently demonstrated ongoing denitrification in sputum from patients infected with p. aeruginosa. therefore we aimed to investigate, whether the pathogenicity of several known cf pathogens is correlated to their ability to perform denitrifi ...201525441256
genomic insights into intrinsic and acquired drug resistance mechanisms in achromobacter xylosoxidans.achromobacter xylosoxidans is an opportunistic pathogen known to be resistant to a wide range of antibiotics; however, the knowledge about the drug resistance mechanisms is limited. we used a high-throughput sequencing approach to sequence the genomes of the a. xylosoxidans type strain atcc 27061 and a clinical isolate, a. xylosoxidans x02736, and then we used different bioinformatics tools to analyze the drug resistance genes in these bacteria. we obtained the complete genome sequence for a. xy ...201525487802
development of a growth-dependent selection system for identification of l-threonine aldolases.threonine aldolases (tas) are useful enzymes for the synthesis of β-hydroxy-α-amino acids due to their capability to catalyze asymmetric aldol reactions. starting from two prochiral compounds, an aldehyde and glycine, two chiral stereocenters were formed in a single step via c-c bond formation. owing to poor diastereoselectivity and low activity, the enzymatic synthesis of β-hydroxy-α-amino acids by tas is still a challenge. for identification of new tas, a growth-dependent selection system in p ...201525616526
stenotrophomonas, achromobacter, and nonmelioid burkholderia species: antimicrobial resistance and therapeutic strategies.stenotrophomonas maltophilia, achromobacter xylosoxidans, and nonmelioid burkholderia species, namely, burkholderia cepacia complex, collectively are a group of troublesome nonfermenters. although not inherently virulent organisms, these environmental gram negatives can complicate treatment in those who are immunocompromised, critically ill in the intensive care unit and those patients with suppurative lung disease, such as cystic fibrosis. through a range of intrinsic antimicrobial resistance m ...201525643274
isolation of multiple drug-resistant enteric bacteria from feces of wild western lowland gorilla (gorilla gorilla gorilla) in gabon.prevalence of drug-resistant bacteria in wildlife can reveal the actual level of anthropological burden on the wildlife. in this study, we isolated two multiple drug-resistant strains, gg6-2 and gg6-1-1, from 27 fresh feces of wild western lowland gorillas in moukalaba-doudou national park, gabon. isolates were identified as achromobacter xylosoxidans and providencia sp., respectively. minimum inhibitory concentrations of the following 12 drugs-ampicillin (abpc), cefazolin (cez), cefotaxime (ctx ...201525649412
efficacy of antimicrobials against biofilms of achromobacter and pseudomonas.achromobacter xylosoxidans and pseudomonas aeruginosa biofilms can develop in ophthalmic products and accessories such as contact lens cases, leading to the development of ocular infections. this study evaluated the efficacy of the antimicrobials polyaminopropyl biguanide (papb) and benzalkonium chloride (bak) against a. xylosoxidans and p. aeruginosa biofilms.201525785528
intrapatient diversity of achromobacter spp. involved in chronic colonization of cystic fibrosis airways.achromobacter spp. are increasingly identified in cystic fibrosis (cf) patients and their ability to persistently colonize the cf respiratory tract (cfrt) suggests that achromobacter species possess adaptive characteristics. we studied genome dynamics in 118 isolates recovered from 13 patients with achromobacter chronic colonization (5-26 isolates per patient recovered over 13-61 months). isolates were identified to species level by nrda gene sequencing, subjected to pulsed-field gel electrophor ...201525791931
biotic inactivation of the pseudomonas aeruginosa quinolone signal molecule.in pseudomonas aeruginosa, quorum sensing (qs) regulates the production of secondary metabolites, many of which are antimicrobials that impact on polymicrobial community composition. consequently, quenching qs modulates the environmental impact of p. aeruginosa. to identify bacteria capable of inactivating the qs signal molecule 2-heptyl-3-hydroxy-4(1h)-quinolone (pqs), a minimal medium containing pqs as the sole carbon source was used to enrich a malaysian rainforest soil sample. this yielded a ...201525809238
pan-resistant achromobacter xylosoxidans and stenotrophomonas maltophilia infection in cystic fibrosis does not reduce survival after lung transplantation.the number of cystic fibrosis (cf) patients undergoing lung transplantation continues to grow, as does the prevalence of multidrug-resistant (mdr) gram-negative rods. however, the posttransplant survival of patients with mdr pathogens, specifically pan-resistant achromobacter xylosoxidans and stenotrophomonas maltophilia, is poorly characterized.201525856407
emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis.chronic suppurative lower airway infection is a hallmark feature of cystic fibrosis (cf). decades of experience in clinical microbiology have enabled the development of improved technologies and approaches for the cultivation and identification of microorganisms from sputum. it is increasingly apparent that the microbial constituents of the lower airways in cf exist in a dynamic state. indeed, while changes in prevalence of various pathogens occur through ageing, differences exist in successive ...201525881770
the crystal structure of d-threonine aldolase from alcaligenes xylosoxidans provides insight into a metal ion assisted plp-dependent mechanism.threonine aldolases catalyze the pyridoxal phosphate (plp) dependent cleavage of threonine into glycine and acetaldehyde and play a major role in the degradation of this amino acid. in nature, l- as well as d-specific enzymes have been identified, but the exact physiological function of d-threonine aldolases (dtas) is still largely unknown. both types of enantio-complementary enzymes have a considerable potential in biocatalysis for the stereospecific synthesis of various β-hydroxy amino acids, ...201525884707
hexacyclopeptides secreted by an endophytic fungus fusarium solani n06 act as crosstalk molecules in narcissus tazetta.the basis of chemical crosstalk in plants and associated endophytes lies in certain so-called communication molecules that are responsible for plant-microbe and microbe-microbe interactions. consequently, elucidating the factors that affect the nature, distribution, and amount of these molecules and how they impact the interaction among endophytes and associated organisms is essential to understand the true potential of endophytes. in the present study, we report the discovery of nine hexacyclop ...201525958995
resonance raman spectra of five-coordinate heme-nitrosyl cytochromes c': effect of the proximal heme-no environment.five-coordinate heme nitrosyl complexes (5cno) underpin biological heme-no signal transduction. bacterial cytochromes c' are some of the few structurally characterized 5cno proteins, exhibiting a distal to proximal 5cno transition of relevance to no sensing. establishing how 5cno coordination (distal vs proximal) depends on the heme environment is important for understanding this process. recent 5cno crystal structures of alcaligenes xylosoxidans cytochrome c' (axcp) and shewanella frigidimarina ...201525961377
achromobacter xylosoxidans bacteremia and cellulitis: a report of a case.achromobacter xylosoxidans is a rare, opportunistic infection most commonly encountered in immunocompromised patients during hospitalization. primary uncomplicated bacteremia, catheter-associated infections, and pneumonia have been reported as the most common clinical presentations; skin and soft tissue infections from a. xylosoxidans are rare. we describe a case of a. xylosoxidans presenting as cellulitis and bacteremia in an immunocompromised patient.201625973735
discrepancy in maldi-tof ms identification of uncommon gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis.early identification of microbial organisms from respiratory secretions of patients with cystic fibrosis (cf) is important to guide therapeutic decisions. the objective was to compare the accuracy of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) relative to the conventional phenotypic method in identifying common bacterial isolates, including nonfermenting gram-negative bacteria, in a cohort of patients with cf.201525995646
success with extended-infusion meropenem after recurrence of baclofen pump-related achromobacter xylosoxidans meningitis in an adolescent.a 13-year-old female experienced a recurrence of baclofen pump-related central nervous system (cns) infection caused by achromobacter, despite absence of retained foreign material. due to the failure of meropenem (120 mg/kg/d in divided doses every 8 hours and infused over 30 minutes) in the initial infection, the dose was infused over 4 hours during the recurrence. meropenem is an antibiotic for which efficacy is time dependent, and 4-hour versus 30-minute infusions have been shown to prolong t ...201526033796
enhanced chrysene degradation by a mixed culture biorem-cgbd using response surface design.degradation of chrysene, a four ringed highly carcinogenic polycyclic aromatic hydrocarbon (pah) has been demonstrated by bacterial mixed culture biorem-cgbd comprising achromobacter xylosoxidans, pseudomonas sp. and sphingomonas sp., isolated from crude oil polluted saline sites at bhavnagar coast, gujarat, india. a full factorial central composite design (ccd) using response surface methodology (rsm) was applied to construct response surfaces, predicting 41.93% of maximum chrysene degradation ...201526040022
hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal no adduct in cytochrome c'.cytochromes c', that occur in methanotrophic, denitrifying and photosynthetic bacteria, form unusual proximal penta-coordinate no complexes via a hexa-coordinate distal no intermediate. their no binding properties are similar to those of the eukaryotic no sensor, soluble guanylate cyclase, for which they provide a valuable structural model. previous studies suggested that hydrogen bonding between the displaced proximal histidine (his120) ligand (following its dissociation from heme due to trans ...201526100643
achromobacter xylosoxidans infection in an adult cystic fibrosis unit in madrid.achromobacter xylosoxidans is an emerging pathogen in cystic fibrosis (cf). although the rate of colonization by this microorganism is variable, prevalence is increasing in cf units.201626139304
a retrospective international study on primary extranodal marginal zone lymphoma of the lung (balt lymphoma) on behalf of international extranodal lymphoma study group (ielsg).primary lymphoma of the lung is a rare entity. clinical features, optimal treatment, role of surgery and outcomes are not well defined, and the follow-up is variable in published data. clinical data of 205 patients who were confirmed to have bronchus mucosa-associated lymphoid tissue lymphoma from december 1986 to december 2011 in 17 different centres worldwide were evaluated. fifty-five per cent of the patients were female. the median age at diagnosis was 62 (range 28-88) years. only 9% had a h ...201626152851
fatal non-thrombotic pulmonary embolization in a patient with undiagnosed factitious disorder.factitious fever is extremely challenging to diagnose in patients with complicated chronic medical problems, and represents as much as 10% of fevers of unknown origin. factitious fever caused by self-injecting oral medications through indwelling central catheters is a diagnostic challenge.201526164684
characterization of two achromobacter xylosoxidans isolates from patients with pertussis-like symptoms.to characterize two achromobacter xylosoxidans recovered from 2 patients diagnosed with pertussis during a bordetella pertussis surveillance program.201526194831
changing epidemiology of the respiratory bacteriology of patients with cystic fibrosis.monitoring potential changes in the epidemiology of cystic fibrosis (cf) pathogens furthers our understanding of the potential impact of interventions.201626203598
sequencing of plasmids pambl1 and pambl2 from pseudomonas aeruginosa reveals a blavim-1 amplification causing high-level carbapenem resistance.carbapenemases are a major concern for the treatment of infectious diseases caused by gram-negative bacteria. although plasmids are responsible for the spread of resistance genes among these pathogens, there is limited information on the nature of the mobile genetic elements carrying carbapenemases in pseudomonas aeruginosa.201526209313
biotransformation of arsenite and bacterial aox activity in drinking water produced from surface water of floating houses: arsenic contamination in cambodia.the potential arsenite bioteansformation activity of arsenic was investigated by examining bacterial arsenic arsenite-oxidizing gene such as aoxs, aoxr, aoxa, aoxb, aoxc, and aoxd in high arsenic-contaminated drinking water produced from the surface water of floating houses. there is a biogeochemical cycle of activity involving arsenite oxidase aox system and the ars (arsenic resistance system) gene operon and aoxr leader gene activity in alcaligenes faecalis srr-11 and aoxs leader gene activity ...201526219073
draft genome sequence of triclosan-resistant cystic fibrosis isolate achromobacter xylosoxidans cf304.achromobacter xylosoxidans is an emerging opportunistic pathogen. here, we present the genome sequence of cystic fibrosis isolate cf304. assembly resulted in 29 contigs adding up to 6.3 mbp. this is the second genome sequence for a cystic fibrosis isolate, and little is known about the genetic basis of pathogenicity in this organism.201526227609
quality control testing for tracking endotoxin-producing gram-negative bacteria during the preparation of polyvalent snake antivenom immunoglobulin.snake bites represent a serious public health problem, particularly in rural areas worldwide. antitoxic sera preparations are antibodies from immunized animals and are considered to be the only treatment option. the purification of antivenom antibodies should aim at obtaining products of consistent quality, safety, efficacy, and adherence to good manufacturing practice principles. endotoxins are an integral component of the outer cell surface of gram-negative bacteria. they are common contaminat ...201726242786
uncommon pathogen: serious manifestation: a rare case of achromobacter xylosoxidans septic arthritis in immunocompetetant patient.achromobacter xylosoxidans is a rare opportunistic gram-negative bacilli and rarer etiology of septic arthritis. we present here the first indian case of septic arthritis due to a. xylosoxidans in 11-month-old male child confirmed by 16s rrna sequencing. the child was admitted as suspected case of septic arthritis and underwent arthrotomy. drained pus revealed gram-negative bacilli, identified as serratia odorifera by api (biomérieux, marcy l'ιtoile, france), later subjected to vitek 2 (biomérie ...201526275277
complete genome sequence of achromobacter xylosoxidans mn001, a cystic fibrosis airway isolate.the genome of achromobacter xylosoxidans mn001, a strain isolated from sputum derived from an adult cystic fibrosis patient, was sequenced using combined single-molecule real-time and illumina sequencing. assembly of the complete genome resulted in a 5,876,039-bp chromosome, representing the smallest a. xylosoxidans genome sequenced to date.201526294635
successful treatment of multiresistant achromobacter xylosoxidans bacteremia in a child with acute myeloid leukemia.achromobacter xylosoxidans is an aerobic gram-negative bacillus and important cause of bacteremia in immunocompromised patients. we describe a leukemia pediatric patient with severe neutropenia who developed bacteremia with a xylosoxidans resistant to multiple antibiotics, and treated the patient with tigecycline and piperacillin-tazobactam in addition to supportive medications.201526336026
achromobacter causing a thrombophlebitis and osteomyelitis combination: a rare cause.achromobacter xylosoxidans is a gram-negative, aerobic bacillus, present in normal human flora of the skin and gastrointestinal tract. infections due to achromobacter are infrequent and have mostly been reported in immunocompromised patients. rarely, however, the microorganism can cause soft tissue infections even in healthy subjects with a history of trauma. we report thrombophlebitis complicated with osteomyelitis secondary to achromobacter in a 15-year-old girl with a history of purulent disc ...201526347234
effect of volatile organic compounds from bacteria on nematodes.the five studied bacterial strains could produce volatile organic compounds (vocs) that kill nematodes. based on their 16s rrna sequences, these strains were identified as pseudochrobactrum saccharolyticum, wautersiella falsenii, proteus hauseri, arthrobacter nicotianae, and achromobacter xylosoxidans. the bacterial vocs were extracted using solid-phase micro-extraction (spme) and subsequently identified by gc/ms analysis. the vocs covered a wide range of aldehydes, ketones, alkyls, alcohols, al ...201526363885
characterization of achromobacter species in cystic fibrosis patients: comparison of bla(oxa-114) pcr amplification, multilocus sequence typing, and matrix-assisted laser desorption ionization-time of flight mass spectrometry.molecular methodologies were used to identify 28 achromobacter spp. from patients with cystic fibrosis (cf). multilocus sequence typing (mlst) identified 17 achromobacter xylosoxidans isolates (all bla(oxa-114) positive), nine achromobacter ruhlandii isolates (all bla(oxa-114) positive), one achromobacter dolens isolate, and one achromobacter insuavis isolate. all less common species were misidentified as a. xylosoxidans by matrix-assisted laser desorption ionization-time of flight mass spectrom ...201526400790
comparative genomics of non-pseudomonal bacterial species colonising paediatric cystic fibrosis patients.the genetic disorder cystic fibrosis is a life-limiting condition affecting ∼70,000 people worldwide. targeted, early, treatment of the dominant infecting species, pseudomonas aeruginosa, has improved patient outcomes; however, there is concern that other species are now stepping in to take its place. in addition, the necessarily long-term antibiotic therapy received by these patients may be providing a suitable environment for the emergence of antibiotic resistance. to investigate these issues, ...201526401445
use of nrda gene sequence clustering to estimate the prevalence of different achromobacter species among cystic fibrosis patients in the uk.we aimed to estimate the prevalence of different achromobacter species among uk cystic fibrosis (cf) patients.201626412052
achromobacter xylosoxidans bloodstream infection in elderly patient with hepatocellular carcinoma: case report and review of literature.achromobacter xylosoxidansis a nonfermentative gram-negative organism, known to cause opportunistic infection in humans. we report a case of septicemia in a 76-year-old male patient with underlying hepatocellular carcinoma due to a. xylosoxidans, which showed a different antimicrobial susceptibility pattern from what is usually reported. from aerobic blood culture of the patient, a. xylosoxidans was isolated which was found to be sensitive to amoxicillin-clavulanic acid, piperacillin-tazobactam, ...201726417165
sequence and structure-based comparative analysis to assess, identify and improve the thermostability of penicillin g acylases.penicillin acylases are enzymes employed by the pharmaceutical industry for the manufacture of semi-synthetic penicillins. there is a continuous demand for thermostable and alkalophilic enzymes in such applications. we have carried out a computational analysis of known penicillin g acylases (pgas) in terms of their thermostable nature using various protein-stabilizing factors. while the presence of disulfide bridges was considered initially to screen putative thermostable pgas from the database, ...201526419382
effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria.enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ...201526464607
recurrent septic arthritis due to achromobacter xylosoxidans in a patient with granulomatosis with polyangiitis.we report a case of recurrent achromobacter xylosoxidans infections including bacteremia, sepsis, septic joints and endocarditis in a 72 year old female with granulomatosis with polyangiitis. achromobacter xylosoxidans is a gram negative bacteria increasingly identified in immunocompromised patients. surgical and medical therapy may need to be combined.201526566537
purification and characterization of exopolysaccharide bioflocculant produced by heavy metal resistant achromobacter xylosoxidans.optimization of process parameters enhanced bioflocculating activity of 'achromobacter xylosoxidans strain teri l1' from 75% to 83.3% in absence of heavy metals, which decreased to 73% in presence of multi-metals. 'teri l1' could adsorb 90% of multi-metals when grown in presence of 1250 mg l(-1) zn, 2 mg l(-1) cd, 30 mg l(-1) pb, 200 mg l(-1) ni and 90 mg l(-1) cu and could adsorb 1100 mg l(-1) of pb when grown in presence of 1500 ppm lead nitrate. the bioflocculant was purified and characterize ...201626686149
efficacy of polysaccharide from alcaligenes xylosoxidans msa3 administration as protection against γ-radiation in female rats.damage to normal tissues is a consequence of both therapeutic and accidental exposures to ionizing radiation. a water-soluble heteropolysaccharide called axeps, composed of glucose, galactose, rhamnose and glucouronic acid in a molar ratio of nearly 1.0:1.6:0.4:2.3, respectively, was isolated from culture medium of strain alcaligenes xylosoxidans msa3 by ethanol precipitation followed by freeze-drying. chemical analysis, fourier-transform infrared (ftir) and chromatographic studies revealed that ...201626712796
maldi-tof mass spectrometry: a powerful tool for clinical microbiology at hôpital principal de dakar, senegal (west africa).our team in europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms). to evaluate the utility of maldi-tof ms in tropical africa in collaboration with local teams, we installed an apparatus in the hôpital principal de dakar (senegal), performed routine identification of isolates, and confirmed or completed their identification in france. in the case of discordance or a la ...201526716681
osteomyelitis caused by achromobacter xylosoxidans.achromobacter xylosoxidans is an aerobic, nonfermenting gram-negative rod and described as a waterborne bacterium since it habits aquatic environments ubiquitously. it has frequently been isolated from aquatic surroundings in the hospital and from various human body sites. although occasionally considered a non-pathogen, a. xylosoxidans has been associated with outbreaks of nosocomial infection due to contaminated fluids. moreover, a wide variety of infectious etiologies due to a. xylosoxidans h ...201526793447
rationale and prospects of targeting bacterial two-component systems for antibacterial treatment of cystic fibrosis patients.bacterial respiratory infections are the main reason of morbidity and mortality among cystic fibrosis (cf) patients. in early childhood, the respiratory infections are due to staphylococcus aureus and haemophilus influenzae. in older cf patients, pathogenic gram-negative bacteria like achromobacter xylosoxidans, burkholderia cepacia complex and especially pseudomonas aeruginosa are more frequently seen. p. aeruginosa is a turning point in the respiratory disease in cf and its predominance increa ...201626853320
assessment of the microbial constituents of the home environment of individuals with cystic fibrosis (cf) and their association with lower airways infections.cystic fibrosis (cf) airways are colonized by a polymicrobial community of organisms, termed the cf microbiota. we sought to define the microbial constituents of the home environment of individuals with cf and determine if it may serve as a latent reservoir for infection.201626859493
impact of delftia tsuruhatensis and achromobacter xylosoxidans on escherichia coli dual-species biofilms treated with antibiotic agents.recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as delftia tsuruhatensis and achromobacter xylosoxidans, might interact with conventional pathogenic agents such as escherichia coli. here, single- and dual-species biofilms of these microorganisms were characterized in terms of microbial composition over time, the average fitness of e. coli, the spatial organization and the biofilm antimicrobial profile. the results reveale ...201626901701
characterization of a novel achromobacter xylosoxidans specific siphoviruse: phiaxp-1.bacteriophages have recently been considered as an alternative biocontrol tool because of the widespread occurrence of antimicrobial-resistant achromobacter xylosoxidans. herein, we isolated a virulent bacteriophage (phiaxp-1) from a water sample of the bohai sea of china that specifically infects a. xylosoxidans. transmission electron microscopy revealed that phage phiaxp-1 belongs to the siphoviridae. we sequenced the genome of phiaxp-1, which comprises 45,045 bp with 64 open reading frames. m ...201626908262
clinical impact of achromobacter xylosoxidans colonization/infection in patients with cystic fibrosis.the rate of diagnosis of colonization/infection of the airways with achromobacter xylosoxidans has increased in cystic fibrosis patients, but its clinical significance is still controversial. this retrospective, case-control study aimed to evaluate the clinical impact of a. xylosoxidans colonization/infection in cystic fibrosis patients. individuals who were chronically colonized/infected (n=10), intermittently colonized/infected (n=15), and never colonized/infected with a. xylosoxidans (n=18) w ...201626909788
[cellulitis due to achromobacter xylosoxidans during bortezomib therapy for multiple myeloma].achromobacter xylosoxidans (a. xylosoxidans) is a non-fermentative gram-negative rod. this organism is reportedly a causative pathogen of bacteremia mainly in patients with hematological disorders. however, only one case of cellulitis due to a. xylosoxidans associated with hematological malignancy has been reported. an 80-year-old man developed cellulitis and subsequent bacteremia due to a. xylosoxidans during bortezomib therapy for multiple myeloma. although his condition was serious enough to ...201626935636
clinical features, antibiotic susceptibility profiles, and outcomes of infectious keratitis caused by achromobacter xylosoxidans.reports on achromobacter xylosoxidans ocular infections are increasing, drawing attention to its emerging role in infectious keratitis. the purpose of this study is to report the clinical features, antibiotic sensitivities, and visual outcomes of infectious keratitis secondary to achromobacter xylosoxidans.201626989957
achromobacter xylosoxidans as a new microorganism strain colonizing high-density polyethylene as a key step to its biodegradation.this study presents results of research on isolation new bacteria strain achromobacter xylosoxidans able to effect on the structure of high-density polyethylene (hdpe), polymer resistant to degradation in environment. new strain of a. xylosoxidans pe-1 was isolated from the soil and identified by analysis of the 16s ribosome subunit coding sequences. the substance to be degraded was hdpe in the form of thin foil films. the foil samples were analyzed with attenuated total reflectance fourier tran ...201627072033
draft genome sequence of the broad-spectrum xenobiotic degrader achromobacter xylosoxidans adaf13.achromobacter xylosoxidansadaf13, isolated from farmland soil, possesses a large number of putative degradation genes and pathways that break down a wide variety of aromatic hydrocarbons, pesticides, endocrine disruptors, and other high-impact xenobiotics. these properties make this strain an excellent candidate for further development as a broad-spectrum bioremediation agent.201627081123
isolation and molecular characterisation of achromobacter phage phiaxp-3, an n4-like bacteriophage.achromobacter xylosoxidans, an opportunistic pathogen, is responsible for various nosocomial and community-acquired infections. we isolated phiaxp-3, an n4-like bacteriophage that infects a. xylosoxidans, from hospital waste and studied its genomic and biological properties. transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiaxp-3 has features characteristic of podoviridae bacteriophages (order caudovirales). with a burst s ...201627094846
a case of septic shock caused by achromobacter xylosoxidans in an immunocompetent female patient after extracorporeal shock wave lithotripsy for a ureteral stone.achromobacter xylosoxidans can cause various types of infections, but its infection in humans is rare. a. xylosoxidans has been reported as a rare etiological agent of infections including primary bacteremia, catheter-related bloodstream infection, endocarditis, otitis, and pneumonia, particularly in immunocompromised hosts. we encountered a case of septic shock caused by a. xylosoxidans in a 52-year-old, immunocompetent woman with no underlying disease, who received extracorporeal shock wave li ...201627104016
deep sequencing approach for investigating infectious agents causing fever.acute undifferentiated fever (auf) poses a diagnostic challenge due to the variety of possible aetiologies. while the majority of aufs resolve spontaneously, some cases become prolonged and cause significant morbidity and mortality, necessitating improved diagnostic methods. this study evaluated the utility of deep sequencing in fever investigation. dna and rna were isolated from plasma/sera of auf cases being investigated at cairns hospital in northern australia, including eight control samples ...201627180244
soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement.since 1960s, the organophosphate pesticide chlorpyrifos has been widely used for the purpose of pest control. however, given its persistence and toxicity towards life forms, the elimination of chlorpyrifos from contaminated sites has become an urgent issue. for this process bioremediation is the method of choice.201727266625
characterization and pathogenicity assessment of gut-associated microbes of muga silkworm antheraea assamensis helfer (lepidoptera: saturniidae).antheraea assamensis helfer (muga silkworm) is an economically important endemic insect species of north eastern region of india. the silkworm is often susceptible to infection by pathogenic bacteria, leads to a disease commonly known as flacherie which causes 40% loss per annum to the silk industry. in this study, we have reported isolation, characterization and pathogenicity assessment of gut-associated bacteria of healthy and diseased muga silkworms. thirty five bacterial isolates were screen ...201627311898
epidemic achromobacter xylosoxidans strain among belgian cystic fibrosis patients and review of literature.achromobacter xylosoxidans is increasingly being recognized as an emerging pathogen in cystic fibrosis. recent severe infections with a. xylosoxidans in some of our cystic fibrosis (cf) patients led to a re-evaluation of the epidemiology of cf-associated a. xylosoxidans infections in two belgian reference centres (antwerp and ghent). several of these patients also stayed at the rehabilitation centre de haan (rhc). in total, 59 a. xylosoxidans isolates from 31 patients (including 26 cf patients), ...201627342812
taxonomic dissection of achromobacter denitrificans coenye et al. 2003 and proposal of achromobacter agilis sp. nov., nom. rev., achromobacter pestifer sp. nov., nom. rev., achromobacter kerstersii sp. nov. and achromobacter deleyi sp. nov.the phenotypic and genotypic characteristics of a historical collection of strains identified as achromobacter denitrificanswere examined. sequence analysis of a 765 bp nrda gene fragment revealed that eight of these strains belonged to the recently described achromobacter aegrifaciens, achromobacter mucicolens, and achromobacter insolitus, and that one strain belonged to achromobacter xylosoxidans. the analysis also suggested the presence of four novel species of the genus achromobacter among t ...201627373279
severe achromobacter xylosoxidans infection and loss of sputum bacterial diversity in an adult patient with cystic fibrosis.achromobacter spp. are emerging pathogens in the lungs of patients with cystic fibrosis. we report the case of an adult patient with cystic fibrosis and chronic a. xylosoxidans infection who experienced rapid, progressive clinical deterioration. metagenomic analysis of the sputum revealed that the airway microbiota was almost entirely dominated by a. xylosoxidans. we review the impact of this organism on lung function and the airway microbiome in cystic fibrosis, and discuss the potential for cr ...201627374622
multi-locus sequence analysis for identification of achromobacter xylosoxidans from blood culture. 201727376508
interspecies dissemination of a mobilizable plasmid harboring blaimp-19 and the possibility of horizontal gene transfer in a single patient.carbapenemase-producing gram-negative bacilli have been a global concern over the past 2 decades because these organisms can cause severe infections with high mortality rates. carbapenemase genes are often carried by mobile genetic elements, and resistance plasmids can be transferred through conjugation. we conducted whole-genome sequencing (wgs) to demonstrate that the same plasmid harboring a metallo-β-lactamase gene was detected in two different species isolated from a single patient. metallo ...201627381397
outbreak of achromobacter xylosoxidans and ochrobactrum anthropi infections after prostate biopsies, france, 2014.we report an outbreak of healthcare-associated prostatitis involving rare environmental pathogens in immunocompetent patients undergoing transrectal prostate biopsies at hôpital édouard herriot (lyon, france) during august 13-october 10, 2014. despite a fluoroquinolone-based prophylaxis, 5 patients were infected with achromobacter xylosoxidans and 3 with ochrobactrum anthropi, which has not been reported as pathogenic in nonimmunocompromised persons. all patients recovered fully. analysis of the ...201627434277
effect of metal ions and petrochemicals on bioremediation of chlorpyrifos in aerobic sequencing batch bioreactor (asbr).application of chlorpyrifos (cp) has increased its environmental concentration. increasing cp concentration has increased chances of adverse health effects. its removal from environment has attained researcher's attention. cp degrading bacterial strains were isolated from wastewater and agricultural soil. finally, selected five bacterial strains were identified using 16s rrna nucleotide sequence analysis as pseudomonas kilonensis srk1, serratia marcescens srk2, bacillus pumilus srk4, achromobact ...201627470246
sepsis caused by achromobacter xylosoxidans in a child with cystic fibrosis and severe lung disease.background achromobacter xylosoxidans is an aerobic, motile, gram-negative, opportunistic pathogen that can be responsible for various severe nosocomial and community-acquired infections. it has been found in immunocompromised patients and patients with several other underlying conditions, but the clinical role of this microorganism in cystic fibrosis is unclear. case report we describe a case of septic shock caused by a. xylosoxidans in a 10-year-old child with cystic fibrosis and severe lung d ...201627498677
essential oil from origanum vulgare completely inhibits the growth of multidrug-resistant cystic fibrosis pathogens.essential oils (eos) are known to inhibit the growth of a wide range of microorganisms. particularly interesting is the possible use of eos to treat multidrug-resistant cystic fibrosis (cf) pathogens. we tested the essential oil (eo) from origanum vulgare for in vitro antimicrobial activity, against three of the major human opportunistic pathogens responsible for respiratory infections in cf patients; these are methicillin-resistant staphylococcus aureus, stenotrophomonas maltophilia and achromo ...201627534136
achromobacter xylosoxidans is the predominant achromobacter species isolated from diverse non-respiratory samples.achromobacter spp. are emerging opportunistic gram-negative rods responsible for diverse nosocomial or community-acquired infections. we describe, for the first time, the distribution of achromobacter spp., defined by nrda gene sequencing, and their antimicrobial susceptibility in a variety of non-respiratory samples recovered from hospitalized patients from 2010 to 2015. of the 63 isolates studied, a. xylosoxidans was the most prevalent (41 isolates), and with the exception of a. insuavis (four ...201627535588
properties of achromobacter xylosoxidans highly resistant to aminoglycoside antibiotics.we herein discovered a highly resistant clinical isolate of pseudomonas aeruginosa with mics to amikacin, gentamicin, and arbekacin of 128 μg/ml or higher in a drug sensitivity survey of 92 strains isolated from the specimens of yoka hospital patients between january 2009 and october 2010, and achromobacter xylosoxidans was separated from this p. aeruginosa isolate. the sensitivity of this bacterium to 29 antibiotics was investigated. the mics of this a. xylosoxidans strain to 9 aminoglycoside a ...201627544979
berberine is a novel type efflux inhibitor which attenuates the mexxy-mediated aminoglycoside resistance in pseudomonas aeruginosa.the emergence and spread of multidrug-resistant p. aeruginosa infections is of great concern, as very few agents are effective against strains of this species. methanolic extracts from the coptidis rhizoma (the rhizomes of coptis japonica var. major satake) or phellodendri cortex (the bark of phellodendron chinense schneider) markedly reduced resistance to anti-pseudomonal aminoglycosides (e.g., amikacin) in multidrug-resistant p. aeruginosa strains. berberine, the most abundant benzylisoquinoli ...201627547203
removal of polychlorinated biphenyl congeners in mixture delor 103 from wastewater by ozonation vs/and biological method.polychlorinated biphenyls (pcbs) produced in slovakia as a commercial mixture delor 103 cause the main contamination of sediment, water and fish in the eastern part of slovakia. delor 103 is a mixture of 40% pcb congeners, nine of them: pcb 8 (2,4'-dichlorobiphenyl), pcb 28 (2,4,4'-trichlorobiphenyl), pcb 52 (2,2',5,5'-tetrachlorobiphenyl), pcb 101 (2,2',4,5,5'-pentachlorobiphenyl), pcb 118 (2,3',4,4',5-pentachlorobiphenyl), pcb 138 (2,2',3,4,4',5'-hexachlorobiphenyl), pcb 153 (2,2',4,4',5,5'-he ...201727607933
molecular insight into the dynamic central metabolic pathways of achromobacter xylosoxidans cf-s36 during heterotrophic nitrogen removal processes.organic carbon sources play a significant role in heterotrophic nitrogen consumption. this quintessential exploration is focused on carbon and nitrogen biogeochemical cycles in heterotrophic bacteria, capable of simultaneous nitrification and denitrification (snd). a heterotrophic bacterial strain achromobacter xylosoxidans cf-s36 isolated from domestic wastewater efficiently eliminated ammonia, nitrate and nitrite by utilizing different carbon sources. the type of carbon utilized by strain cf-s ...201727660099
identification and molecular characterization of bacteriophage phiaxp-2 of achromobacter xylosoxidans.a novel achromobacter xylosoxidans bacteriophage, phiaxp-2, was isolated from hospital sewage in china. the phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. its morphology showed that phiaxp-2 belongs to the family siphoviridae. microbiological characterization demonstrated that ph 7 is most suitable for phage phiaxp-2; its titer decreased when the temperature exceeded 50 °c; phiaxp-2 is sens ...201627669904
exposing the three-dimensional biogeography and metabolic states of pathogens in cystic fibrosis sputum via hydrogel embedding, clearing, and rrna labeling.physiological resistance to antibiotics confounds the treatment of many chronic bacterial infections, motivating researchers to identify novel therapeutic approaches. to do this effectively, an understanding of how microbes survive in vivo is needed. though much can be inferred from bulk approaches to characterizing complex environments, essential information can be lost if spatial organization is not preserved. here, we introduce a tissue-clearing technique, termed mipact, designed to retain an ...201627677788
the dynamics behind the affinity: controlling heme-gas affinity via geminate recombination and heme propionate conformation in the no carrier cytochrome c'.nitric oxide (no) sensors are heme proteins which may also bind co and o2. control of heme-gas affinity and their discrimination are achieved by the structural properties and reactivity of the heme and its distal and proximal environments, leading to several energy barriers. in the bacterial no sensor cytochrome c' from alcaligenes xylosoxidans (axcp), the single leu16ala distal mutation boosts the affinity for gas ligands by a remarkable 10(6)-10(8)-fold, transforming axcp from one of the lowes ...201627709886
pseudobacteremia outbreak of biofilm-forming achromobacter xylosoxidans - environmental transmission.achromobacter xylosoxidans (ax) is known for intrinsic resistance to disinfectants. our laboratory routine surveillance system detected an unexpected rise in ax bloodstream infections in a 2200-bed hospital. an epidemiological investigation was conducted to find the source and disrupt further transmission.201627756240
polymicrobial infection of the cornea due to contact lens wear.a 38-year-old male presented with pain and redness in his left eye. he had a history of wearing contact lenses. his ophthalmic examination revealed a large corneal ulcer with surrounding infiltrate. cultures were isolated from the contact lenses, lens solutions, storage cases, and conjunctivae of both eyes and also corneal scrapings of the left eye. fortified vancomycin and amikacin drops were started hourly. culture results of conjunctivae of each eye and left cornea were positive for pseudomon ...201627800266
patterns of virulence factor expression and antimicrobial resistance in achromobacter xylosoxidans and achromobacter ruhlandii isolates from patients with cystic fibrosis.achromobacter spp. are opportunistic pathogens increasingly recovered from adult patients with cystic fibrosis (cf). we report the characterization of 122 achromobacter spp. isolates recovered from 39 cf patients by multilocus sequence typing, virulence traits, and susceptibility to antimicrobials. two species, a. xylosoxidans (77%) and a. ruhlandii (23%) were identified. all isolates showed a similar biofilm formation ability, and a positive swimming phenotype. by contrast, 4·3% and 44·4% of a. ...201727873565
achromobacter aloeverae sp. nov., isolated from the root of aloe vera (l.) burm.f.two gram-staining-negative, strictly aerobic, rod-shaped bacteria, designated strains ava-1t and ava-2, were isolated from the root of aloe vera (l.) brum.f. derived from chachoengsao province, thailand. the strains contained cytochrome oxidase and catalase activities. they grew in 4 % (w/v) nacl, at a ph range of 6.0-9.0 (optimally at ph 7) and at 20-42 °c (optimally at 30-37 °c). the major isoprenoid quinone was ubiquinone with eight isoprene units (q-8). the major fatty acids were c16 : 0 and ...201727902206
erratum to: achromobacter xylosoxidans as a new microorganism strain colonizing high-density polyethylene as a key step to its biodegradation. 201728004373
impact of polymicrobial biofilms in catheter-associated urinary tract infections.recent reports have demonstrated that most biofilms involved in catheter-associated urinary tract infections are polymicrobial communities, with pathogenic microorganisms (e.g. escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae) and uncommon microorganisms (e.g. delftia tsuruhatensis, achromobacter xylosoxidans) frequently co-inhabiting the same urinary catheter. however, little is known about the interactions that occur between different microorganisms and how they impact biofilm f ...201628033847
in vitro activity of ceftolozane-tazobactam against multidrug-resistant nonfermenting gram-negative bacilli isolated from patients with cystic fibrosis.ceftolozane-tazobactam was tested against 58 multidrug-resistant nonfermenting gram-negative bacilli (35 pseudomonas aeruginosa, 11 achromobacter xylosoxydans, and 12 stenotrophomonas maltophilia isolates) isolated from cystic fibrosis patients and was compared to ceftolozane alone, ceftazidime, meropenem, and piperacillin-tazobactam. ceftolozane-tazobactam was the most active agent against p. aeruginosa but was inactive against a. xylosoxydans and s. maltophilia in time-kill experiments, ceftol ...201728096165
nosocomial infections with imp-19-producing pseudomonas aeruginosa linked to contaminated sinks, france.we isolated imp-19-producing pseudomonas aeruginosa from 7 patients with nosocomial infections linked to contaminated sinks in france. we showed that blaimp-19 was located on various class 1 integrons among 8 species of gram-negative bacilli detected in sinks: p. aeruginosa, achromobacter xylosoxidans, a. aegrifaciens, p. putida, stenotrophomonas maltophilia, p. mendocina, comamonas testosteroni, and sphingomonas sp.201728098548
achromobacter xylosoxidans infection in cystic fibrosis siblings with different outcomes: case reports.the clinical relevance of achromobacter xylosoxidans infection in cystic fibrosis (cf) remains controversial. this emerging agent in cf has been associated with increased lung inflammation, more frequent exacerbations and more severe lung disease. we describe a pair of cf siblings chronically colonized by the same multilocus genotype of a. xylosoxidans with different clinical courses, and assess whether this species may have developed any virulence traits and antimicrobial resistance that could ...201728138423
hospital-acquired pneumonia due to achromobacter xylosoxidans in the elderly: a single-center retrospective study in beijing.achromobacter xylosoxidans has been reported in several countries; however, hospital-acquired pneumonia (hap) due to this organism in elderly patients in china remains rare.201728141585
contamination of burn wounds by achromobacter xylosoxidans followed by severe infection: 10-year analysis of a burn unit population.gram-negative infections predominate in burn surgery. until recently, achromobacter species were described as sepsis-causing bacteria in immunocompromised patients only. severe infections associated with achromobacter species in burn patients have been rarely reported. we retrospectively analyzed all burn patients in our database, who were treated at the intensive care burn unit (icbu) of the cologne merheim burn centre from january 2006 to december 2015, focusing on contamination and infection ...201628149253
biocidal efficacy of multipurpose solutions against gram-negative organisms associated with corneal infiltrative events.because corneal infiltrative events (cies) may result from bacterial components on contact lenses, which can come from contaminated lens cases, we evaluated the biocidal efficacy of five multipurpose solutions against gram-negative commonly isolated and cie-associated organisms.201728194876
biofilm formation of achromobacter xylosoxidans on contact lens.achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. the aim of this study was to investigate the possibility of isolated strain of achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (cl), to quantify the production of the formed biofilm, and compare it with the reference strains (pseudomonas aeruginosa, staphylococcus aureus, and haemophilus influenzae). bacterial strain isolated from one contact lens case w ...201728218002
achromobacter xylosoxidans septic arthritis in a child with primary immunodeficiency. 201728250627
bacteria associated with marginal zone lymphomas.in the last decades some bacteria have been associated with a various extent with marginal zone (extra nodal, nodal and splenic types) lymphomas are frequently associated with chronic infections, with important clinical, molecular, biological, and therapeutic implications. the well-known correlation between helicobacter pylori and gastric malt-lymphoma, the recently reported links between chlamydia psittaci and ocular adnexal malt-lymphoma and borrelia burgdorferi and cutaneous malt lymphoma con ...201728288714
environmental distribution and drug susceptibility of achromobacter xylosoxidans isolated from outdoor and indoor environments.achromobacter xylosoxidans is an environmental bacterium with multi-drug resistance. we isolated achromobacter xylosoxidans and investigated its susceptibility to 13 drugs. seventy-eight water samples were collected from rivers and ponds, and 11 samples were swabbed from residential sinks and baths. nine strains of achromobacter xylosoxidans were isolated from the 89 samples. five strains, including 2 that were sampled from residential homes, showed high resistance to multiple aminoglycosides. t ...201728331426
keratitis due to achromobacter xylosoxidans in a contact lens user.ocular infections due to achromobacter xylosoxidans are extremely uncommon; their diagnosis is a challenge and the optimal treatment remains controversial. we present a case of a. xylosoxidans in a contact lens user and a review of the literature to facilitate diagnostic suspicion and empirical therapeutic management.201728350622
characterization and genome comparisons of three achromobacter phages of the family siphoviridae.in this study, we present the characterization and genomic data of three achromobacter phages belonging to the family siphoviridae. phages 83-24, jwx and jwf were isolated from sewage samples in paris and braunschweig, respectively, and infect achromobacter xylosoxidans, an emerging nosocomial pathogen in cystic fibrosis patients. analysis of morphology and growth parameters revealed that phages 83-24 and jwx have similar properties, both have nearly the same head and tail measurements, and both ...201728357512
diagnosis of biofilm infections in cystic fibrosis patients.chronic pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. the initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. the matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (dna, oxygen radicals and proteases). ...201728407432
case report: the first case of achromobacter xylosoxidans-related tunnel infection in a patient receiving peritoneal dialysis.achromobacter xylosoxidans infection is mostly reported in immunocompromised patients. until now, it is still rarely reported in patients undergoing peritoneal dialysis.201728422870
prevalence and outcomes of achromobacter species infections in adults with cystic fibrosis: a north american cohort study.achromobacter species are increasingly being detected in cystic fibrosis (cf) patients, with an unclear epidemiology and impact. we studied a cohort of patients attending a canadian adult cf clinic who had positive sputum cultures for achromobacter species in the period from 1984 to 2013. infection was categorized as transient or persistent (≥50% positive cultures for 1 year). those with persistent infection were matched 2:1 with age-, sex-, and time-matched controls without a history of achromo ...201728446570
engineering proximal vs. distal heme-no coordination via dinitrosyl dynamics: implications for no sensor design.proximal vs. distal heme-no coordination is a novel strategy for selective gas response in heme-based no-sensors. in the case of alcaligenes xylosoxidans cytochrome c' (axcp), formation of a transient distal 6cno complex is followed by scission of the trans fe-his bond and conversion to a proximal 5cno product via a putative dinitrosyl species. here we show that replacement of the axcp distal leu16 residue with smaller or similar sized residues (ala, val or ile) traps the distal 6cno complex, wh ...201728451315
hypertonic saline as a useful tool for sputum induction and pathogen detection in cystic fibrosis.the aim of this study was to compare the qualitative and semi-quantitative detection of pathogens in the airway secretions of patients with cystic fibrosis (cf) and the sputum induction capacity before and after inhalation of 7% hypertonic saline solution (hss).201728455785
septic arthritis of the temporomandibular joint secondary to acute otitis media in an adult: a rare case with achromobacter xylosoxidans.septic arthritis of the temporomandibular joint (satmj) is a rare complication of acute otitis media (aom) with only four reported cases in the english and japanese literature. based on the unusual nature of this clinical condition, we discuss the first documented case due to achromobacter xylosoxidans and the utility of myringotomy with long-term intravenous antibiotics via a peripherally inserted central catheter (picc). we describe the case of a 76-year-old male patient that was brought in by ...201728458938
differences in gene expression profiles between early and late isolates in monospecies achromobacter biofilm.bacteria of genus achromobacter are emerging pathogens in cystic fibrosis (cf) capable of biofilm formation and development of antimicrobial resistance. evolutionary adaptions in the transition from primary to chronic infection were assessed by transcriptomic analysis of successive isolates of achromobacter xylosoxidans from a single cf patient. several efflux pump systems targeting antimicrobial agents were upregulated during the course of the disease, whereas all genes related to motility were ...201728534862
seasonality of acquisition of respiratory bacterial pathogens in young children with cystic fibrosis.seasonal variations are often observed for respiratory tract infections; however, limited information is available regarding seasonal patterns of acquisition of common cystic fibrosis (cf)-related respiratory pathogens. we previously reported differential seasonal acquisition of pseudomonas aeruginosa in young children with cf and no such variation for methicillin-susceptible staphylococcus aureus acquisition. the purpose of this study was to describe and compare the seasonal incidence of acquis ...201728599639
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