Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients. | the accurate and rapid identification of bacteria isolated from the respiratory tract of patients with cystic fibrosis (cf) is critical in epidemiological studies, during intrahospital outbreaks, for patient treatment, and for determination of therapeutic options. while the most common organisms isolated from sputum samples are pseudomonas aeruginosa, staphylococcus aureus, and haemophilus influenzae, in recent decades an increasing fraction of cf patients has been colonized by other nonfermenti ... | 2008 | 18550747 |
| observations of metabolite formation and variable yield in thiodiglycol biodegradation process : impact on reactor design. | the complete microbial degradation of thiodiglycol (tdg), the primary hydrolysis product of sulfur mustard, by alcaligenes xylosoxydans ssp. xylosoxydans (sh91) was accomplished in laboratory-scale stirredtank reactors. an andrews substrate inhibition model was used to describe the cell growth. the yield factor was not constant, but a relationship with initial substrate concentration has been developed. using a substrate-inhibition and variable-yield kinetic model, we can describe the cell growt ... | 1997 | 18576129 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| [detection methods and changes in metallo-beta-lactamase-producing gram-negative rod isolates at nagasaki university hospital from 1991 to 2005]. | since plasmid-mediated metallo-beta-lactamase (mbl)-producing pseudimonas aeruginosa was reported in japan, mbl-producing gram-negative rods (gnrs) have emerged worldwide. we developed a way to detect mbl-producing gnrs in routine examination using broth microdilution with the mbl inhibitor sodium mercaptoacetate (sma) in frozen plates. between 1996 and 2005, we evaluated this and other methods, including broth microdilution with another mbl inhibitor dipicolinic acid (dpa) in dry plates, conven ... | 2008 | 18697478 |
| [isolation and characterization of a new glyphosate-resistant strain from extremely polluted environment]. | to isolate and characterize a glyphosate-resistant strain from extremely polluted environment. | 2008 | 18720850 |
| on-line optical and x-ray spectroscopies with crystallography: an integrated approach for determining metalloprotein structures in functionally well defined states. | x-ray-induced redox changes can lead to incorrect assignments of the functional states of metals in metalloprotein crystals. the need for on-line monitoring of the status of metal ions (and other chromophores) during protein crystallography experiments is of growing importance with the use of intense synchrotron x-ray beams. significant efforts are therefore being made worldwide to combine different spectroscopies in parallel with x-ray crystallographic data collection. here the implementation a ... | 2008 | 18728313 |
| emerging pathogens in cystic fibrosis: ten years of follow-up in a cohort of patients. | in patients with cystic fibrosis (cf), there is an increasing incidence of some uncommon respiratory pathogens, such as burkholderia cepacia complex (bcc), stenotrophomonas maltophilia, and achromobacter xylosoxidans. in order to evaluate the prevalence and the clinical impact of these pathogens, we retrospectively studied a total of 109 patients followed in our center from 1996 to 2006 and reviewed the results of 1,550 sputum samples. the isolation of pseudomonas aeruginosa slightly decreased o ... | 2009 | 18758832 |
| a comparative study of avian bordetella-like strains, bordetella bronchiseptica, alcaligenes faecalis and other related nonfermentable bacteria. | cultural and biochemical characteristics, electrophoretic patterns of soluble proteins, and pathogenicity in turkey poults, of 30 avian gram-negative nonfermentable bacterial strains acquired from various geographical regions, of which 26 had been isolated from turkeys and one each from a chicken, duck, goose and sharp-tailed munia, were compared to those of bordetella bronchiseptica, alcaligenes faecalis, alcaligenes denitrificans, achromobacter xylosoxidans and bordetella parapertussis. with t ... | 1983 | 18766782 |
| thermotemporal dynamics of contaminant bacteria and antimicrobials in extended porcine semen. | bacterial contamination of extended porcine semen has been associated with deleterious effects on both semen quality and sow fertility. retrospective, prospective and in vitro studies were performed to delineate the prevalence and behavior of certain bacterial contaminants in extended semen, and antimicrobial pharmacodynamics in various semen diluents. retrospective review of extended semen samples submitted from north american boar studs for microbiological screening at the university of pennsy ... | 2008 | 18774598 |
| diversity of bacteria that nodulate prosopis juliflora in the eastern area of morocco. | a total of 274 bacterial strains were isolated from the root nodules of prosopis juliflora, growing in two arid soils of the eastern area of morocco. a physiological plate screening allowed the selection of 15 strains that could tolerate nacl concentrations between 175 and 500 mm. these were compared with 15 strains chosen from among the ones which did not tolerate high salinity. the diversity of strains was first assessed by rep-pcr amplification fingerprinting using boxa1r and eric primers. an ... | 2008 | 18789623 |
| isolation of microorganisms and substances inhibitory to aflatoxin production. | aflatoxins are toxic and carcinogenic secondary metabolites produced by aspergillus flavus and a. parasiticus. the contamination of crops, feeds, and foods with aflatoxins can have serious effects on the health of humans and animals. although many studies have been done to develop aflatoxin-control strategies, most are limited in their effectiveness. as part of an effort to develop control procedures, we have devised simple and safe methods that are useful for identifying microorganisms that eff ... | 2008 | 18798037 |
| risa-hplc analysis of lung bacterial colonizers of cystic fibrosis children. | microbiological analysis of sputum samples, from children affected by cystic fibrosis (cf) and showing signs of acute or chronic infections, is routinely performed by culture-dependent approaches involving selective media and biochemical tests. these identification schemes are time-consuming, and may lead to false negative results. the aim of this work was to evaluate the efficacy of a ribosomal intergenic spacer analysis (risa) coupled to high performance liquid chromatography (hplc) for the de ... | 2009 | 18929602 |
| fate of a genetically modified bacterium in foregut of glassy-winged sharpshooter (hemiptera: cicadellidae). | symbiotic control is a new strategy being investigated to prevent the spread of insect-transmitted pathogens by reducing vector competence. we are developing this strategy to reduce the spread of xylella fastidiosa by homalodisca vitripennis (germar) [formerly homalodisca coagulata (say)] (hemiptera: cicadellidae), the glassy-winged sharpshooter. in this study, the fate of a transformed symbiotic bacterium, alcaligenes xylosoxidans variety denitriicans (s1axd), in the foregut of glassy-winged sh ... | 2008 | 18950032 |
| achromobacter xylosoxidans respiratory tract infections in cystic fibrosis patients. | achromobacter xylosoxidans is a ubiquitous gram-negative non-fermenting rod, recently characterized as an emerging pathogen in cystic fibrosis (cf) patients. its pathogenic potential and prevalent transmission routes are still unclear. this study investigated the pfge genetic pattern and antimicrobial resistance profile of 42 a. xylosoxidans isolates obtained over 4 years from the respiratory tract of 22 cf patients. by genotypic analysis, 31 isolates were attributed to 8 distinct pfge patterns ... | 2008 | 19024606 |
| alcaligenes xylosoxidans endophthalmitis following phacoemulsification and intraocular lens implantation. | five consecutive cases of endophthalmitis that developed after cataract extraction by a single surgeon using the same operating room during one morning session are described. following preoperative topical administration of ciprofloxacin, surgery consisted of phacoemulsification with peristaltic pump and fluid venting, polymethylmethacrylate intraocular lens implantation, and corneal suture. no complications occurred during surgery. all five patients developed endophthalmitis caused by infection ... | 2008 | 19065983 |
| biodegradation of methyl tert-butyl ether using bacterial strains. | prospective methyl tert-butyl ether (mtbe) degrading bacterial strains and/or consortia were identified. the potential for aerobic degradation of mtbe was examined using bacterial isolates from contaminated soils and groundwater. using the 16s rdna protocol, two isolates capable of degrading mtbe (rhodococcus pyridinivorans 4a and achromobacter xylosoxidans 6a) were identified. the most efficient consortium of microorganisms was acquired from contaminated groundwater. the growth of both strains ... | 2008 | 19085075 |
| alcaligenes xylosoxidans cholecystitis and meningitis acquired during bathing procedures in a burn unit: a case report. | the information in this article was presented at the 37th annual meeting of the japan society of burn, nagoya, japan, june 7-8, 2008. alcaligenes xylosoxidans, a nonfermentative, gram-negative rod often found in aqueous environments, has been isolated from respirators, incubators, and disinfectant solutions in the hospital environment. it is known to cause disease in immunocompromised (eg, burn) patients and represents a cross-contamination risk related to wound care. in the authors' burn unit, ... | 2008 | 19104123 |
| degradation and mineralization of 2-chloro-, 3-chloro- and 4-chlorobiphenyl by a newly characterized natural bacterial strain isolated from an electrical transformer fluid-contaminated soil. | a bacterium classified as achromobacter xylosoxidans strain ir08 by phenotypic typing coupled with 16s rrna gene analysis was isolated from a soil contaminated with electrical transformer fluid for over sixty years using aroclor 1221 as an enrichment substrate. the substrate utilization profiles revealed that ir08 could grow on all three monochlorobiphenyls (cbs), 2,4'- and 4,4'-dichlorobiphenyl as well as 2-chlorobenzoate (2-cba), 3-cba, 4-cba, and 2,3-dichlorobenzoate. unusually, growth was po ... | 2008 | 19143351 |
| new insights into the biodegradation of thiodiglycol, the hydrolysis product of yperite (sulfur mustard gas). | to isolate thiodiglycol (tdg)-degrading bacteria, the mustard gas hydrolysis product, and to characterize the metabolites formed and the enzymes involved in the degradation. | 2009 | 19191966 |
| nickel(ii)-substituted azurin i from alcaligenes xylosoxidans as characterized by resonance raman spectroscopy at cryogenic temperature. | metal-substituted blue copper proteins (cupredoxins) have been successfully used to study the effect of metal-ion identity on their active-site properties, specifically the coordination geometry and metal-ligand bond strengths. in this work, low-temperature (77 k) resonance raman (rr) spectra of the blue copper protein alcaligenes xylosoxidans azurin i and its ni(ii) derivative are reported. a detailed analysis of all observed bands is presented and responsiveness to metal substitution is discus ... | 2009 | 19224256 |
| characterization of novel plant growth promoting endophytic bacterium achromobacter xylosoxidans from wheat plant. | nine diazotrophic bacteria were isolated from surface-sterilized roots and culms of wheat variety malviya-234, which is grown with very low or no inputs of nitrogen fertilizer. out of the nine bacteria, four showed indole acetic acid (iaa) production, and five were positive for p solubilization. one isolate, wm234c-3, showed appreciable level of nitrogenase activity, iaa production, and p solubilization ability, and was further characterized with a view to exploiting its plant growth promoting a ... | 2009 | 19224271 |
| biodegradation and detoxification of endosulfan in aqueous medium and soil by achromobacter xylosoxidans strain cs5. | achromobacter xylosoxidans cs5, capable of utilizing endosulfan as the sole carbon, sulfur and energy source, was isolated from the activated sludge. degradation of endosulfan by strain cs5 was examined by hplc. analysis of culture ph, cells growth, and residual endosulfan demonstrated that cs5 could degrade more than 24.8 mg/l alpha-endosulfan and 10.5mg/l beta-endosulfan after 8 days in aqueous medium, with the formation of endosulfan diol and endosulfan ether as the major metabolites. cell-fr ... | 2009 | 19237238 |
| low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients. | pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ... | 2009 | 19261796 |
| achromobacter xylosoxidans, an emerging pathogen in catheter-related infection in dialysis population causing prosthetic valve endocarditis: a case report and review of literature. | dialysis catheter-related infection is a major cause of morbidity and mortality in patients on dialysis. in recent years, there have been reported cases of infections with opportunistic environmental organism, achromobacter xylosoxidans (ax) causing bacteremia in patients on dialysis. however, no previous such reports on prosthetic valve endocarditis in a dialysis patient with achromobacter xylosoxidans were found after a medline search. we report such a case and review the literature. | 2009 | 19281752 |
| effects of organoclays on soil eubacterial community assessed by molecular approaches. | the aim of this study was to evaluate the effects of the commercial organoclays, cloisite 30b, nanofil 804 and dellite 26c on soil eubacterial community. an enrichment test was carried out on nutrient broth containing the organoclay and the microorganisms previously isolated from soil. four transfers were made, each after 7 days incubation. the molecular analyses on the eubacterial community were performed before treatment and 7 days after each transfer. dna was extracted, amplified with eubacte ... | 2009 | 19304378 |
| microbiological spectrum of organisms isolated from explanted scleral buckles. | scleral buckle removal is an uncommon procedure performed for various reasons. microbiological information on explanted scleral buckles remains limited. the authors identified 37 cases of scleral buckle removal during an 18-year period. bacterial cultures isolated an organism in 4 of 9 patients (44%) with clinical infection. organisms identified included methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa, mycobacterium abscessus, and coagulase-negative staphylococcus species. ba ... | 2009 | 19320315 |
| activation parameters for heme-no binding in alcaligenes xylosoxidans cytochrome c': the putative dinitrosyl intermediate forms via a dissociative mechanism. | the bacterial heme protein alcaligenes xylosoxidans cytochrome c' (axcp) forms a novel five-coordinate heme-nitrosyl (5c-no) complex in which no resides at the proximal heme face in place of the endogenous protein ligand. intriguingly, axcp shares no-binding properties with the eukaryotic no-sensor, soluble guanylate cyclase (sgc), including 5c-no formation via two no-dependent reactions. for both proteins, a model has been proposed in which no binds to the vacant distal face to form a transient ... | 2009 | 19334778 |
| structures of various cytochromes c evaluated from the redox behaviors using the optically active co(iii) complex-modified au electrode. | electrochemical studies of three c-type cytochromes (cyt c from horse heart, cyt c(2) from rhodospirillum rubrum, and cyt c(553) from alcaligenes xylosoxidans gifu 1051) were performed by using the optically active co(iii) complex-modified au electrode. three cytochromes gave different redox behaviors reflected from the respective structural information. from relationship between their redox behaviors and their structural characteristics, we evaluated the solution structure around heme center of ... | 2007 | 19356022 |
| epidemiological typing of clinical isolates of achromobacter xylosoxidans: comparison of phenotypic and genotypic methods. | the purpose of this paper is to evaluate the utility of different typing methods for achromobacter xylosoxidans clinical isolates. ninety-two blood culture isolates of a. xylosoxidans subsp. xylosoxidans were collected over a 25-month period. the typeability, discriminatory power and reproducibility of commonly used phenotypic and genotypic methods, such as resistotyping, plasmid profiling, whole-cell protein fingerprinting, random amplification of polymorphic dna (rapd) and pulsed-field gel ele ... | 2009 | 19373499 |
| in this issue: proteomics 8/2009. | in this issue of proteomics you will find the following highlighted articles:are you sick or are you just getting old: does it matter?we all joke about the hazards of aging: various systems that break down, some you didn't even know you had. but then there's the alternative of not aging. hmmm. now what if aging were a disease? if so, it is worse than any cold i've had. zürbig et al. have found that the proteome of the aging kidney has many markers in common with chronic kidney disease. the degre ... | 2009 | 19382142 |
| bacterial consortium proteomics under 4-chlorosalicylate carbon-limiting conditions. | in this study, the stable consortium composed by pseudomonas reinekei strain mt1 and achromobacter xylosoxidans strain mt3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. the analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. carbon-limiting studies showed a higher metabolic versatility in the community through upreg ... | 2009 | 19382143 |
| unusual microbes in asthma exacerbation: alcaligenes xylosoxidans and leishmania. | asthma is a chronic inflammatory condition characterised by a variable degree of airflow limitation. exacerbations during the course of asthma often occur due to environmental factors or infectious, mostly viral, aetiology. the present study reports the case of a 61-yr-old male with severe asthma hospitalised due to increasing respiratory distress. since recovery was delayed despite anti-obstructive/anti-inflammatory and antibiotic therapy, further diagnostic procedures, including bronchoscopy, ... | 2009 | 19407055 |
| [achromobacter xylosoxidans colonization in bronchiectasis]. | 2010 | 19446928 | |
| [conjunctivitis due to achromobacter xylosoxidans: case report]. | we report here a case of conjunctivitis in an immunocompetent patient due to achromobacter xylosoxidans, which was associated with the use of rigid contact lenses. the bacteria were isolated from the scraped conjunctival swab as well as from the lens cleaning fluid. a. xylosoxidans is an opportunistic pathogen, especially in immunocompromised patients; however, in isolates of ocular infections, from immunocompetent patients, it may be confused with other gram-negative organisms, particularly pse ... | 2009 | 19466343 |
| alcaligenes xylosoxidans keratitis after autologous cultivated limbal epithelium transplant. | 2009 | 19491995 | |
| respiratory microbiology of patients with cystic fibrosis in the united states, 1995 to 2005. | numerous improvements in diagnostic and therapeutic strategies for patients with cystic fibrosis (cf) have occurred during the past 2 decades. we hypothesized that these changes could impact trends in respiratory microbiology. | 2009 | 19505987 |
| multiple drug-resistant alcaligenes xylosoxidans keratitis in a sanitation worker. | to report a case of daily wear soft contact lens-associated keratitis caused by alcaligenes xylosoxidans in a portable toilet sanitation worker. | 2009 | 19516143 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis. | broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples. | 2009 | 19570210 |
| cough-generated aerosols of pseudomonas aeruginosa and other gram-negative bacteria from patients with cystic fibrosis. | pseudomonas aeruginosa is the most common bacterial pathogen in patients with cystic fibrosis (cf). current infection control guidelines aim to prevent transmission via contact and respiratory droplet routes and do not consider the possibility of airborne transmission. it was hypothesised that subjects with cf produce viable respirable bacterial aerosols with coughing. | 2009 | 19574243 |
| demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases. | the reduction of nitrite (no2-) into nitric oxide (no), catalyzed by nitrite reductase, is an important reaction in the denitrification pathway. in this study, the catalytic mechanism of the copper-containing nitrite reductase from alcaligenes xylosoxidans (axnir) has been studied using single and multiple turnover experiments at ph 7.0 and is shown to involve two protons. a novel steady-state assay was developed, in which deoxyhemoglobin was employed as an no scavenger. a moderate solvent kinet ... | 2009 | 19586913 |
| association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in taiwan. | this study was designed to determine the strength of the association between the isolation of nonfermentative gram-negative bacilli (nfgnb) from tap water faucet aerators and the prevalence of colonization or infection of patients in intensive care units (icus). surveillance cultures were obtained during a 4-month period from 162 faucet aerators located in seven different icus. the prevalence of colonization or infection of icu patients with nfgnb was determined by prospective surveillance durin ... | 2009 | 19587299 |
| complicated intra-abdominal infection caused by extended drug-resistant achromobacter xylosoxidans. | achromobacter xylosoxidans (formerly alcaligenes xylosoxidans) is a rare but important nosocomial pathogen. antibiotic resistance has been increasing during the past decade. a. xylosoxidans may be confused with pseudomonas spp. but, unlike pseudomonas spp., this organism has peritrichous flagella. complicated intra-abdominal infection with a. xylosoxidans has rarely been reported in the literature. this report is of an immunocompetent patient with acute cholecystitis complicated by an intra-abdo ... | 2009 | 19597652 |
| parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor. | a novel betaproteobacterium, designated strain ch07(t), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in south korea. in order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. the new strain was gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16s rrna gene sequence analysis revealed that strain ch07(t) belonged to the class betaproteobacteria, being ... | 2010 | 19671719 |
| real-time electrochemical monitoring of the polymerase chain reaction by mediated redox catalysis. | we described the proof-of-principle of a nonoptical real-time pcr that uses cyclic voltammetry for indirectly monitoring the amplified dna product generated in the pcr reaction solution after each pcr cycle. to enable indirect measurement of the amplicon produced throughout pcr, we monitor electrochemically the progressive consumption (i.e., the decrease of concentration) of free electroactive deoxynucleoside triphosphates (dntps) used for dna synthesis. this is accomplished by exploiting the fa ... | 2009 | 19722651 |
| clinical, microbiological profile and treatment outcome of ocular infections caused by achromobacter xylosoxidans. | to study the microbiological profile and outcome of ocular infections caused by achromobacter xylosoxidans. | 2009 | 19730091 |
| eurocarecf quality assessment of diagnostic microbiology of cystic fibrosis isolates. | the identification of microbial species from respiratory specimens and their susceptibility to antimicrobial agents are among the most important diagnostic measures of care for patients with cystic fibrosis (cf). under the umbrella of eurocarecf, two quality assurance trials of cf microbiology were performed in 2007 and 2008. nine formulations with cf bacterial isolates were dispatched. a total of 31/37 laboratories from 18/21 european countries participated in the 2007 and 2008 trials. the comm ... | 2009 | 19741077 |
| [epidemiological survey of bacteria isolated from the respiratory tract of cystic fibrosis patients]. | with the aim of characterizing the evolution of the epidemiological profile of respiratory bacterial infections of patients having cystic fibrosis (cf), the authors conducted a retrospective analysis about it's incidence and prevalence in 78 cf patients followed at the cf specialized centre, paediatric department, santa maria hospital, lisbon, during a 5 years period (1995-1999). pseudomonas aeruginosa was the most frequently isolated bacteria during the first three years of the study (60-73%), ... | 2003 | 19771691 |
| [psoas and paravesical abscess formation due to achromobacter xylosoxidans: a case report]. | 2010 | 19775779 | |
| incidence, clinical features, causative organisms, and visual outcomes of delayed-onset pseudophakic endophthalmitis. | to report the incidence, clinical features, causative organisms, and visual outcomes associated with delayed-onset pseudophakic endophthalmitis. | 2009 | 19787601 |
| [alcaligenes xylosoxidans bacteremia in a patient with acute lymphoblastic leukemia]. | alcaligenes xylosoxidans which is an aerobic, non-fermentative gram-negative bacillus found in aqueous environments and human flora, can lead to opportunistic infections. it causes infections in elderly, immunocompromised patients, patients with chronic disorders or premature infants. in this report, a case of a. xylosoxidans bacteremia that developed in a child with acute lymphoblastic leukemia (all) was presented. four-years-old male patient under all induction therapy was admitted with sympto ... | 2009 | 19795625 |
| in vitro pharmacodynamics of levofloxacin and other aerosolized antibiotics under multiple conditions relevant to chronic pulmonary infection in cystic fibrosis. | the inhalational administration of antibiotics can provide high concentrations locally in the lungs of cystic fibrosis patients and, thus, can be useful for the treatment of chronic bacterial infections. the present study evaluated the in vitro activities of levofloxacin, ciprofloxacin, tobramycin, amikacin, and aztreonam against clinical isolates of pseudomonas aeruginosa, burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and staphylococcus aureus from cystic ... | 2010 | 19805554 |
| a case of endocarditis of difficult diagnosis in dialysis: could "pest" friends be involved? | we report a case of a. xylosoxidans endocarditis of larvate clinical presentation in a central venous catheter-dependent hemodialysis patient of difficult diagnosis and poor evolution despite a high index of suspicion and consequent assessment. a 50-year-old man on hemodialysis presented with inflammatory-malnutrition parameters during the months prior to diagnosis of endocarditis, whilst he was otherwise asymptomatic. no vegetations were detectable on his cardiac valves at repeated echocardiogr ... | 2009 | 19863886 |
| estimating the cellular maintenance coefficient and its use in the design of two-phase partitioning bioscrubbers. | one of the key roles of an organic solvent has emerged to be the enhancement of oxygen transfer in two-phase partitioning bioscrubbers (tppbs). in order to determine an optimum organic fraction for a given vocs loading, the oxygen demand of the total cell mass must be estimated, which depends upon the magnitude of the cellular maintenance coefficient. we have estimated the dynamics of the maintenance coefficient for benzene degradation by achromobacter xylosoxidans y234 in a tppb and found that ... | 2010 | 19915871 |
| inflammation in achromobacter xylosoxidans infected cystic fibrosis patients. | achromobacter xylosoxidans infection may cause conspicuous chronic pulmonary inflammation in cystic fibrosis (cf) patients similar to pseudomonas aeruginosa and the burkholderia cepacia complex (bcc). evolution in lung function was compared in chronically infected patients. cytokine concentrations in cf patients with and without chronic infection were compared to healthy controls. | 2010 | 19939747 |
| achromobacter xylosoxidans presenting as a suprapatellar abscess and polyarthritis. | 2010 | 20051759 | |
| achromobacter xylosoxidans genomic characterization and correlation of randomly amplified polymorphic dna profiles with relevant clinical features [corrected] of cystic fibrosis patients. | achromobacter xylosoxidans is an emerging pathogen increasingly being isolated from respiratory samples of cystic fibrosis (cf) patients. its role and clinical significance in lung pathogenesis have not yet been clarified. the aim of the present study was to genetically characterize a. xylosoxidans strains isolated from cf patients by use of randomly amplified polymorphic dna (rapd) profiles and to look for a possible correlation between rapd profiles and the patients' clinical features, such as ... | 2010 | 20107101 |
| isolate-specific effects of patulin, penicillic acid and edta on biofilm formation and growth of dental unit water line biofilm isolates. | dental unit water line (duwl) contamination by opportunistic pathogens has its significance in nosocomial infection of patients, health care workers, and life-threatening infections to immunocompromized persons. recently, the quorum sensing (qs) system of duwl isolates has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. in this study, the effect of two quorum-sensing inhibitory compounds (patulin; pat, penicillic acid; pa) and edta on ... | 2010 | 20111864 |
| screening of bacterial isolates from polluted soils exhibiting catalase and peroxidase activity and diversity of their responses to oxidative stress. | for the survival of individual isolates of gram-negative bacteria pseudomonas putida, achromobacter xylosoxidans, and the gram-positive bacterium bacillus megaterium, in an environment polluted with crude oil products, the production of catalases exhibiting both catalase and dianisidine-peroxidase activity is important. electrophoretic resolution of cell-free extracts of aerobically grown strains in luria-bertani medium during exponential phase revealed distinctive expression of catalatic and pe ... | 2010 | 20145932 |
| characterization of phosphobacteria isolated from eutrophic aquatic ecosystems. | phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. to develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. molecular identification and phylogenetic analysis revealed three groups among the nine isolates of inorganic phosphate-solubilizing bacteria (ipsb): ipsb1 and ipsb2 belonged to the actinobacteria and flavobacteria, respectively, and the other seven belonged to the gamma-proteobacteria. among f ... | 2009 | 20170019 |
| characterization of cycp gene expression in achromobacter xylosoxidans ncimb 11015 and high-level heterologous synthesis of cytochrome c' in escherichia coli. | the cycp gene encoding a periplasmic cytochrome c' from the denitrifying beta-proteobacterium achromobacter xylosoxidans was characterized. the genes flanking cycp encode components of a mobile genetic element characteristic of the beta-proteobacteria, suggesting that cycp has inserted within a transposon or insertion element. the gene therefore does not form part of a denitrification operon or gene cluster. the level of expression of the cycp gene and the level of synthesis of its corresponding ... | 2010 | 20185933 |
| changes in cystic fibrosis sputum microbiology in the united states between 1995 and 2008. | the study objective was to identify changes in cystic fibrosis (cf) sputum microbiology over 13 years. | 2010 | 20232473 |
| endophytic bacteria improve seedling growth of sunflower under water stress, produce salicylic acid, and inhibit growth of pathogenic fungi. | endophytic bacterial strains sf2 (99.9% homology with achromobacter xylosoxidans), and sf3 and sf4 (99.9% homology with bacillus pumilus) isolated from sunflower grown under irrigation or drought were selected on the basis of plant growth-promoting bacteria (pgpb) characteristics. aims of the study were to examine effects of inoculation with sf2, sf3, and sf4 on sunflower cultivated under water stress, to evaluate salicylic acid (sa) production by these strains in control medium or at ψa = -2.03 ... | 2010 | 20383767 |
| consortia modulation of the stress response: proteomic analysis of single strain versus mixed culture. | the high complexity of naturally occurring microbial communities is the major drawback limiting the study of these important biological systems. in this study, a comparison between pure cultures of pseudomonas reinekei sp. strain mt1 and stable community cultures composed of mt1 plus the addition of achromobacter xylosoxidans strain mt3 (in a steady-state proportion 9:1) was used as a model system to study bacterial interactions that take place under simultaneous chemical and oxidative stress. b ... | 2010 | 20406297 |
| in vitro synergistic activity of combined piperacillin and tobramycin against clinical strains of achromobacter xylosoxidans. | 2010 | 20435577 | |
| bilateral achromobacter xylosoxidans keratitis after laser in situ keratomileusis. | a 31-year-old man was referred to us 2 months after bilateral laser in situ keratomileusis (lasik). on presentation, the corrected distance visual acuity was hand motion in the right eye and 20/25 in the left eye. slitlamp examination showed a diffuse central stromal infiltrate, flap melting, and hypopyon in the right eye and marked interface opacities with crystal-like edges in the left eye. flap lift and irrigation were performed. because of the progressive keratitis, penetrating keratoplasty ... | 2010 | 20494781 |
| newer antibacterial agents and their potential role in cystic fibrosis pulmonary exacerbation management. | pulmonary exacerbations in cystic fibrosis (cf) are frequent events and account for a substantial proportion of the burden of morbidity and mortality in this disease. antibacterial therapies to treat pulmonary exacerbations are instituted empirically and are individualized based on both patient factors (severity of exacerbation, frequency of exacerbation, recent courses of anti-infectives) and pathogen factors (previously isolated pathogens and in vitro predicted susceptibilities). however, the ... | 2010 | 20605846 |
| capd peritonitis caused by alcaligenes xylosoxidans in a diabetic cirrhosis patient. | despite the advancement of disconnecting system, peritonitis remains one of the most common causes in patients treated with continuous ambulatory peritoneal dialysis (capd). we report a cirrhosis patient undergoing capd who suffered from an episode of peritonitis caused by a rare pathogen, alcaligenes xylosoxidans (ax). this organism rarely induces capd peritonitis and only nine cases were reported in the literature. this is the first case of ax-related capd peritonitis in cirrhosis patient. inf ... | 2010 | 20662708 |
| [microbial contamination of nipradiol with and without benzalkonium chloride]. | to compare microbial contamination of nipradiol both with and without benzalkonium chloride (bac). | 2010 | 20681256 |
| a strategic approach for the design and operation of two-phase partitioning bioscrubbers for the treatment of volatile organic compounds. | a strategic approach for the design of two-phase partitioning bioscrubbers (tppbs) has been formulated using, as a basis, a re-evaluation of extensive literature data available for the degradation of benzene by achromobacter xylosoxidans y234 in tppbs with n-hexadecane as the partitioning phase. using a previously determined maintenance coefficient for benzene, we determined that an inlet benzene loading rate of 100 mg/h requires 5,928 mg cell mass at biological steady state and 243.0 mg o(2) /h ... | 2010 | 20718037 |
| [achromobacter xylosoxidans colonisation in bronchiectasis]. | 2010 | 20846757 | |
| selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases. | a microbial community was selected for growth on dried and naoh-treated switchgrass. during a 14-day liquid cultivation, a 70% loss in dry weight was observed during the first 4 days and after 14 days, the hemicellulose and cellulose in the system were degraded by 73.5% and 67.3%, respectively. the carboxymethyl cellulase (cmcase) and xylanase levels reached 0.21 and 3.75 iu, respectively. the optimal ph for cmcase and xylanase activities was 5 and 6, respectively. the optimal reaction temperatu ... | 2010 | 20933405 |
| cytochromes: reactivity of the "dark side" of the heme. | ligand binding to the heme distal side is a paradigm of heme-protein biochemistry, the proximal axial ligand being in most cases a his residue. no binds to the ferrous heme-fe-atom giving rise to hexa-coordinated adducts (as in myoglobin and hemoglobin) with his and no as proximal and distal axial ligands, respectively, or to penta-coordinated adducts (as in soluble guanylate cyclase) with no as the axial distal ligand. recently, the ferrous derivative of alcaligenes xylosoxidans cytochrome c' ( ... | 2010 | 20952122 |
| new vector tools with a hygromycin resistance marker for use with opportunistic pathogens. | the ability of many bacterial strains to tolerate antibiotics can limit the number of molecular tools available for research of these organisms. to help address this problem, we have modified a diverse set of vectors to include a broadly expressed hygromycin resistance (hmr) marker. hygromycin b is an aminoglycoside antibiotic not used to treat infections in humans and has antimicrobial activity against a wide range of microorganisms. vectors with four replication origins are represented, with p ... | 2011 | 20972648 |
| distal-to-proximal no conversion in hemoproteins: the role of the proximal pocket. | hemoproteins play central roles in the formation and utilization of nitric oxide (no) in cellular signaling, as well as in protection against nitrosative stress. key to heme-nitrosyl function and reactivity is the fe coordination number (5 or 6). for (five-coordinate) 5c-no complexes, the potential for no to bind on either heme face exists, as in the microbial cytochrome c' from alcaligenes xylosoxidans (axcytcp), which forms a stable proximal 5c-no complex via a distal six-coordinate no interme ... | 2010 | 21073879 |
| an outbreak of achromobacter xylosoxidans associated with ultrasound gel used during transrectal ultrasound guided prostate biopsy. | we describe an outbreak of achromobacter xylosoxidans after transrectal ultrasound guided prostate biopsy at a urology unit at a tertiary care center as well as clinical and microbiological investigation, and intervention. | 2010 | 21074197 |
| complete genome sequence of the haloaromatic acid-degrading bacterium achromobacter xylosoxidans a8. | achromobacter xylosoxidans strain a8 was isolated from soil contaminated with polychlorinated biphenyls. it can use 2-chlorobenzoate and 2,5-dichlorobenzoate as sole sources of carbon and energy. this property makes it a good starting microorganism for further development toward a bioremediation tool. the genome of a. xylosoxidans consists of a 7-mb chromosome and two large plasmids (98 kb and 248 kb). besides genes for the utilization of xenobiotic organic substrates, it contains genes associat ... | 2010 | 21097610 |
| biodegradation of endosulfan and endosulfan sulfate by achromobacter xylosoxidans strain c8b in broth medium. | endosulfan is one of the most widely used wide spectrum cyclodiene organochlorine insecticide. in environment, endosulfan can undergo either oxidation or hydrolysis reaction to form endosulfan sulfate and endosulfan diol respectively. endosulfan sulfate is as toxic and as persistent as its parent isomers. in the present study, endosulfan degrading bacteria were isolated from soil through selective enrichment technique using sulfur free medium with endosulfan as sole sulfur source. out of the 8 i ... | 2010 | 21161332 |
| why two are not enough: degradation of p-toluenesulfonate by a bacterial community from a pristine site in moorea, french polynesia. | in previous work, only one culture (strain ta12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (tsa) as a sole source of carbon and energy for aerobic growth. 'strain ta12' has now been recognized as a community of three bacteria: achromobacter xylosoxidans ta12-a, ensifer adhaerens ta12-b and pseudomonas nitroreducens ta12-c. achromobacter xylosoxidans ta12-a and e. adhaerens ta12-b were identified as the tsa degraders. these two organisms contain sever ... | 2011 | 21204940 |
| achromobacter xylosoxidans respiratory tract infection in cystic fibrosis patients. | the aims of this study were to evaluate the frequency of achromobacter xylosoxidans infection in a cohort of cystic fibrosis patients, to investigate antimicrobial sensitivity, to establish possible clonal likeness among strains, and to address the clinical impact of this infection or colonization on the general outcome of these patients. the study was undertaken between january 2004 and december 2008 on 300 patients receiving care at the regional cystic fibrosis center of the naples university ... | 2011 | 21279730 |
| rapid genotyping of achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia isolates using melting curve analysis of rapd-generated dna fragments (mcrapd). | typing of bacteria is important for monitoring newly emerging pathogens and for examining local outbreaks. we evaluated the randomly amplified polymorphic dna technique in combination with melting curve analysis (mcrapd) of the amplified dna fragments to genotype isolates from five gram-negative species, i.e. achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia. by determining the melting temperature peaks of the ampl ... | 2011 | 21320595 |
| two unique presentations of achromobacter xylosoxidans infections in clinical settings. | two rare and unique infections of achromobacter xylosoxidans are described. the first case is a novel presentation of acute necrotising pancreatitis leading to a pancreatic pseudocyst, which was treated successfully in an immunocompetent male. the second case describes a local wound infection of metastatic ductal carcinoma of the breast; the patient consequently succumbed from a pre-existing co-morbid condition. vigilant and efficient microbiological workup and surveillance are needed to diagnos ... | 2011 | 21389595 |
| proton-coupled electron transfer in the catalytic cycle of alcaligenes xylosoxidans copper-dependent nitrite reductase. | we demonstrated recently that two protons are involved in reduction of nitrite to nitric oxide through a proton-coupled electron transfer (et) reaction catalyzed by the blue cu-dependent nitrite reductase (cu nir) of alcaligenes xylosoxidans (axnir). here, the functionality of two putative proton channels, one involving asn90 and the other his254, is studied using single (n90s, h254f) and double (n90s-h254f) mutants. all mutants studied are active, indicating that protons are still able to reach ... | 2011 | 21469743 |
| identification of achromobacter xylosoxidans by detection of the bla(oxa-114-like) gene intrinsic in this species. | achromobacter xylosoxidans is an emerging pathogen among patients with cystic fibrosis. here we describe a specific pcr for identification of this organism, based on detection of bla(oxa-114-like). comparison of isolates by pulsed-field gel electrophoresis revealed evidence of cross-infection in some cases, but most patients harbored their own strain. | 2011 | 21497041 |
| complicated septic shock caused by achromobacter xylosoxidans bacteremia in a patient with acute lymphoblastic leukaemia. | infections caused by achromobacter xylosoxidans cause significant morbidity and mortality in debilitated individuals. eradication of these infections requires prolonged therapy with antimicrobial agents and removal of any infected central venous catheter. the outcome is usually poor in patients with high risk malignancy, septic complications, and/or multi-organ dysfunction. | 2007 | 21503249 |
| late hematogenous bacterial infections of breast implants: two case reports of unique bacterial infections. | late infections of breast implants are rare occurrences. we present 2 cases of late infections of breast implants, resulting from hematogenous spread from bacterial infection from distant sites. the first case involves a late implant infection following development of a chronic foot sore, caused by achromobacter xylosoxidans, an aerobic pathogen. the second case describes a late infection, after extensive dental treatment, caused by streptococcus viridans, a bacterium that normally lives in clos ... | 2011 | 21508821 |
| revisited distribution of nonfermenting gram-negative bacilli clinical isolates. | nonfermenting gram-negative bacilli (nf-gnb) are ubiquitous environmental opportunistic bacteria frequently misidentified by conventional phenotypic methods. the aim of this study was to determine the distribution of nf-gnb species by 16 s rrna gene sequencing (used as reference method) and to compare performances of biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof-ms). from nine french hospitals, 188 nf-gnb isolates (except p. aerugin ... | 2011 | 21509476 |
| engineering the substrate specificity of alcaligenesd-aminoacylase useful for the production of d-amino acids by optical resolution. | d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6 (axd-naase) offers a novel biotechnological application, the production of d-amino acid from the racemic mixture of n-acyl-dl-amino acids. however, its substrate specificity is biased toward certain n-acyl-d-amino acids. to construct mutant axd-naases with substrate specificities different from those of wild-type enzyme, the substrate recognition site of the axd-naase was rationally manipulated based on computational structural ... | 2011 | 21546325 |
| achromobacter xylosoxidans infection presenting as a pulmonary nodule mimicking cancer: case report and literature review. | achromobacter xylosoxidans is typically isolated from pulmonary sources, presenting as pneumonia in immunosuppressed individuals. we describe a novel clinical presentation of a. xylosoxidans infection presenting as multiple spiculated, pulmonary nodules mimicking cancer for which the patient underwent a wedge resection of the lung for diagnosis and staging of presumptive cancer. | 2011 | 21593259 |
| intragenomic and intraspecific heterogeneity of the 16s rrna gene in seven bacterial species from the respiratory tract of cystic fibrosis patients assessed by pcr-temporal temperature gel electrophoresis. | 16s rrna gene-based cultivation-independent methods are increasingly used to study the diversity of microbiota during health and disease. one bias of these methods is the variability of 16s rrna gene that may exist among strains of a same species (intraspecific heterogeneity) or between rrs copies in a genome (intragenomic heterogeneity). we evaluated the level of intraspecific and intragenomic 16s rdna variability in seven species frequently encountered in respiratory tract samples in cystic fi ... | 2011 | 21621347 |
| a case of meningitis due to achromobacter xylosoxidans denitrificans 60 years after a cranial trauma. | background: achromobacter xylosoxidans (ax) is a non-fermentative aerobic gram-negative bacillus. it is an opportunistic pathogen and the causative agent of various infections. we report an original case of late posttraumatic meningitis due to ax denitrificans.<br /> case report: an 83-year-old man was hospitalized for acute headache, nausea and vomiting. the emergency brain computer tomography (ct) scan did not reveal any anomaly. in his medical history, there was an auditory injury due to a cr ... | 2011 | 21629191 |
| fatal catheter-related bacteremia due to alcaligenes (achromobacter) xylosoxidans in a hemodialysis patient. | 2011 | 21637989 | |
| a comparative study of biodegradation of vinyl acetate by environmental strains. | four gram-negative strains, e3_2001, ec1_2004, ec3_3502 and ec2_3502, previously isolated from soil samples, were subjected to comparative studies in order to select the best vinyl acetate degrader for waste gas treatment. comparison of biochemical and physiological tests as well as the results of fatty acids analyses were comparable with the results of 16s rrna gene sequence analyses. the isolated strains were identified as pseudomonas putida ec3_2001, pseudomonas putida ec1_2004, achromobacter ... | 2011 | 21654921 |
| emerging gram-negative infections in burn wounds. | gram-negative infection remains a major contributor to morbidity, mortality, and cost of care. in the absence of comparative multinational epidemiological studies specific to burn patients, we sought to review literature trends in emerging gram-negative burn wound infections within the past 60 years. mapping trends in these organisms, although in a minority compared with the six "eskape" pathogens currently being targeted by the infectious diseases society of north america, would identify pathog ... | 2011 | 21792068 |
| first description of a rnd-type multidrug efflux pump in achromobacter xylosoxidans: axyabm. | achromobacter xylosoxidans is an emerging pathogen in cystic fibrosis patients. the multidrug resistance of these bacteria remains poorly understood. we have characterized in a clinical strain the first resistance-nodulation-cell division (rnd)-type multidrug efflux pump in this species: axyabm. the inactivation of the transporter component axyb gene led to decreased minimum inhibitory concentrations (mics) of cephalosporins (except cefepime), aztreonam, nalidixic acid, fluoroquinolones and chlo ... | 2011 | 21807978 |
| marked increase in incidence of achromobacter xylosoxidans infections caused by sporadic acquisition from the environment. | background: an increased incidence of achromobacter xylosoxidans infections has been observed at the cystic fibrosis centre at aarhus university hospital, as the proportion of patients colonised with a. xylosoxidans increased from 6 to 10% from 2005 to 2009. methods: pulsed field gel electrophoresis (pfge) was used to type isolates of a. xylosoxidans. results: four patients infected for 2-7years were part of a larger epidemic spread involving both danish cf centres, while 11 patients carried str ... | 2011 | 21835703 |
| achromobacter xylosoxidans: characterization of strains in brazilian cystic fibrosis patients. | we investigated the possibility of cross-infection among cystic fibrosis patients in two brazilian reference centers. achromobacter xylosoxidans isolates (n = 122) were recovered over a 5-year period from 39 patients. isolates were genetically heterogeneous, but one genotype was present in 56% of the patients, suggesting that cross-infection may have occurred. | 2011 | 21849686 |
| enhanced chrysene degradation by halotolerant achromobacter xylosoxidans using response surface methodology. | degradation of chrysene, a four ring high molecular weight (hmw) polycyclic aromatic hydrocarbon (pah) is of intense environmental interest, being carcinogenic, teratogenic and mutagenic. multiple pah degrading halotolerant achromobacter xylosoxidans was isolated from crude oil polluted saline site. response surface methodology (rsm) using central composite design (ccd) of bushnell-haas medium components was successfully employed for optimization resulting 40.79% chrysene degradation on 4th day. ... | 2011 | 21855331 |
| Decolorizing activity of malachite green and its mechanisms involved in dye biodegradation by Achromobacter xylosoxidans MG1. | An Achromobacter xylosoxidans MG1 strainisolated from the effluent treatment plant of a textile and dyeing factory from Yunnan Province in China was found capable of decolorizing the malachite green dye at a high efficacy. Strain MG1 reduced 86% malachite green at the concentration of 2,000 mg/l within 1 h, representing a greater ability for decolorizing and a higher tolerance of this compound than all previously reported bacteria. Color removal was optimal at pH 6 and 38°C. Further experimental ... | 2011 | 21865764 |
| fluorescence lifetime analysis of nitrite reductase from alcaligenes xylosoxidans at the single-molecule level reveals the enzyme mechanism. | 2011 | 21922585 |