Publications

antibiograms of resistant gram-negative bacteria from scottish cf patients.	over a 19-month pilot phase, 93 multiply resistant gram-negative isolates from scottish cystic fibrosis patients were sent to a referral laboratory for further investigation.	2004	15463901
structure-based engineering of alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin.	the intermolecular electron transfer from achromobacter cycloclastes pseudoazurin (acpaz) to wild-type and mutant alcaligenes xylosoxidans nitrite reductases (axnirs) was investigated using steady-state kinetics and electrochemical methods. the affinity and the electron transfer reaction constant (k(et)) are considerably lower between acpaz and axnir (k(m) = 1.34 mm and k(et) = 0.87 x 10(5) m(-1) s(-1)) than between acpaz and its cognate nitrite reductase (acnir) (k(m) = 20 microm and k(et) = 7. ...	2004	15475344
the completely sequenced plasmid pest4011 contains a novel incp1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation.	the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacterium achromobacter xylosoxidans subsp. denitrificans strain est4002 contains plasmid pest4011. this plasmid ensures its host a stable 2,4-d(+) phenotype. we determined the complete 76,958-bp nucleotide sequence of pest4011. this plasmid is a deletion and duplication derivative of pd2m4, the 95-kb highly unstable laboratory ancestor of pest4011, and was self-generated during different laboratory manipulations performed to increas ...	2004	15489427
pitfalls of polymyxin antimicrobial susceptibility testing of pseudomonas aeruginosa isolated from cystic fibrosis patients.	with their potent activity against gram-negative bacteria, the polymyxins are important alternative antibiotics for cystic fibrosis (cf) patients. a retrospective evaluation of polymyxin activity against 6001 pseudomonas aeruginosa, 150 achromobacter xylosoxidans and 506 stenotrophomonas maltophilia cf isolates was initiated. in addition, we looked at how polymyxin susceptibility testing was affected by the testing method (agar dilution versus microdilution), the agent (polymyxin e versus polymy ...	2004	15509615
characterization of two type 1 cu sites of hyphomicrobium denitrificans nitrite reductase: a new class of copper-containing nitrite reductases.	we report (1) the amino acid sequence of hyphomicrobium denitrificans nitrite reductase (hdnir), containing two type 1 cu sites and one type 2 cu site; (2) the expression and preparation of wild-type hdnir and two mutants replacing the cys ligand of each type 1 cu with ala; and (3) their spectroscopic and functional characterization. the open-reading frame of 50-kda hdnir is composed of the 15-kda n-terminal domain having a type 1 cu-binding motif like cupredoxins and the 35-kda c-terminal domai ...	2004	15518568
in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients.	owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ...	2004	15523180
phosphonium ionic liquids for degradation of phenol in a two-phase partitioning bioreactor.	six ionic liquids (ils), which are organic salts that are liquid at room temperature, were tested for their biocompatibility with three xenobiotic-degrading bacteria, pseudomonas putida, achromobacter xylosoxidans, and sphingomonas aromaticivorans. of the 18 pairings, seven were found to demonstrate biocompatibility, with one il (trihexyl(tetradecyl)phosphonium bis(trifluoromethylsulfonyl) amide) being biocompatible with all three organisms. this il was then used in a two-phase partitioning bior ...	2005	15549289
site-directed mutagenesis alters dnak-dependent folding process.	the overproduction of d-aminoacylase (a6-d-anase) of alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) is accompanied by aggregation of the overproduced protein, and its soluble expression is facilitated by the coexpression of dnak-dnaj-grpe (dnakje). when the a6-d-anase gene was expressed in the escherichia coli dnak mutant dnak756, little activity was observed in the soluble fraction, and it was restored by the coexpression of dnakje or the substitution of the r354 residue of ...	2005	15567154
cyclo(l-leucyl-l-prolyl) produced by achromobacter xylosoxidans inhibits aflatoxin production by aspergillus parasiticus.	aflatoxins are potent carcinogenic and toxic substances that are produced primarily by aspergillus flavus and aspergillus parasiticus. we found that a bacterium remarkably inhibited production of norsolorinic acid, a precursor of aflatoxin, by a. parasiticus. this bacterium was identified as achromobacter xylosoxidans based on its 16s ribosomal dna sequence and was designated a. xylosoxidans nfri-a1. a. xylosoxidans strains commonly showed similar inhibition. the inhibitory substance(s) was excr ...	2004	15574949
insights into redox partner interactions and substrate binding in nitrite reductase from alcaligenes xylosoxidans: crystal structures of the trp138his and his313gln mutants.	dissimilatory nitrite reductase catalyses the reduction of nitrite to nitric oxide within the key biological process of denitrification. we present biochemical and structural results on two key mutants, one postulated to be important for the interaction with the partner protein and the other for substrate entry. trp138, adjacent to one of the type-1 cu ligands, is one of the residues surrounding a small depression speculated to be important in complex formation with the physiological redox partn ...	2004	15610025
clonal relatedness and conserved integron structures in epidemiologically unrelated pseudomonas aeruginosa strains producing the vim-1 metallo-{beta}-lactamase from different italian hospitals.	three epidemiologically independent pseudomonas aeruginosa isolates, representative of the first vim-1 metallo-beta-lactamase producers detected at three different hospitals in northern italy, were investigated to determine their genomic relatedness and to compare the structures of the genetic supports for the vim-1 determinants. the three isolates, all of serotype o11, appeared to be clonally related according to the results of genotyping by macrorestriction analysis of genomic dna by pulsed-fi ...	2005	15616282
heterologous metalloprotein biosynthesis in escherichia coli: conditions for the overproduction of functional copper-containing nitrite reductase and azurin from alcaligenes xylosoxidans.	this paper reports on the optimization of conditions for the overproduction and isolation of two recombinant copper metalloproteins, originally encoded on the chromosome of the dentrifying soil bacterium alcaligenes xylosoxidans, in the heterologous host escherichia coli. the trimeric enzyme nitrite reductase (nir) contains both type-1 and type-2 cu centres, whilst its putative redox partner, azurin i, is monomeric and has only a type-1 cu centre. both proteins were processed and exported to the ...	2004	15616359
bacteriospermia in extended porcine semen.	bacteriospermia is a frequent finding in freshly extended porcine semen and can result in detrimental effects on semen quality and longevity if left uncontrolled. the primary source of bacterial contamination is the boar. other sources that have been identified include environment, personnel, and the water used for extender preparation. a 1-year retrospective study was performed on submissions of extended porcine semen for routine quality control bacteriological screening at the university of pe ...	2005	15626417
effect from multiple episodes of inadequate empiric antibiotic therapy for ventilator-associated pneumonia on morbidity and mortality among critically ill trauma patients.	empiric antibiotic therapy is routinely initiated for patients with presumed ventilator-associated pneumonia (vap). the impact of inadequate empiric antibiotic therapy (ieat) may vary among critically ill populations. the purpose of this retrospective study was to determine the effect of ieat on the outcome for adult trauma patients with vap.	2005	15674157
establishment of a genetically marked insect-derived symbiont in multiple host plants.	alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ...	2005	15723145
quantifying maintenance requirements from the steady-state operation of a two-phase partitioning bioscrubber.	an innovative method for directly and explicitly quantifying the maintenance energy requirements of pure cultures growing on volatile organic compound (voc) substrates in a two-phase partitioning bioscrubber is described. direct evidence of maintenance energy requirements of achromobacter xylosoxidans y234 is provided both through observed reductions in the macroscopic biomass-to-substrate yield with decreasing specific growth rates, but more remarkably through achievement of steady-state operat ...	2005	15726578
prospective evaluation of emerging bacteria in cystic fibrosis.	bacteria contribute considerably to the progression of lung disease in cystic fibrosis. in this prospective, multi-centre study, we aimed to evaluate the occurrence of emerging bacteria and the physicians' assessments of the clinical importance of these findings.	2005	15752680
biodegradation of 2,4,6-tribromophenol during transport in fractured chalk.	the effect of physicochemical conditions (residence time, oxygen concentrations, and chalk characteristics) on the biodegradation of 2,4,6-tribromophenol (tbp) during transport was investigated in low-permeability fractured-chalk cores. long-term (approximately 600 d) biodegradation experiments were conducted in two cores (approximately 21 cm diameter, 31 and 44 cm long, respectively), intersected by a natural fracture. tbp was used as a model contaminant and as the sole carbon source for aerobi ...	2005	15757335
role of arginine residues of d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6.	to investigate the role of arginine in the folding of d-aminoacylase, seven arginine residues, r26, r152, r296, r302, r354, r377, and r391, among twelve arginine residues highly conserved in d-aminoacylase, n-acyl-d-aspartate amidohydrolase (d-aaase), and n-acyl-d-glutamate amidohydrolase (d-agase) from alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) were substituted with lysine by site-directed mutagenesis. the mutants, r26k, r152k, r296k, and r302k were identified as mutatio ...	2005	15777281
recurrent alcaligenes xylosoxidans keratitis.	to describe a case of postkeratoplasty recurrent alcaligenes xylosoxidans keratitis.	2005	15829812
[application of molecular systematics to study of bacterial cultures consuming volatile organic compounds].	a range of species of four mixed bacterial cultures was studied by molecular systematics methods with the use of 16s rrna genes. the cultures had been developed for application in minireactors, to degrade volatile organic compounds (vocs): ethyl benzene, m-xylene, styrene, and o-xylene. a sample of 30 plasmid rdna clones was obtained for each of the mixed cultures. the clones were analyzed by rflp according to two restriction sites. major variants of the 16s-rdna sequences, corresponding to the ...	2005	15859460
nitric oxide interaction with cytochrome c' and its relevance to guanylate cyclase. why does the iron histidine bond break?	soluble guanylate cyclase (sgc), the mammalian receptor for nitric oxide (no), is a heme protein with a histidine as the proximal ligand. formation of a five-coordinate heme-no complex with the associated fe-his bond cleavage is believed to trigger a conformational change that activates the enzyme and transduces the no signal. cytochrome c' (cyt c') is a protobacteria heme protein that has several similarities with sgc, including the ability to form a five-coordinate no adduct and the fact that ...	2005	15913362
hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil.	we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ...	2005	15920621
accessibility of the distal heme face, rather than fe-his bond strength, determines the heme-nitrosyl coordination number of cytochromes c': evidence from spectroscopic studies.	the heme coordination chemistry and spectroscopic properties of rhodobacter capsulatus cytochrome c' (rccp) have been compared to data from alcaligenes xylosoxidans (axcp), with the aim of understanding the basis for their different reactivities with nitric oxide (no). whereas ferrous axcp reacts with no to form a predominantly five-coordinate heme-nitrosyl complex via a six-coordinate intermediate, rccp forms an equilibrium mixture of six-coordinate and five-coordinate heme-nitrosyl species in ...	2005	15952773
shared genotypes of achromobacter xylosoxidans strains isolated from patients at a cystic fibrosis rehabilitation center.	during a study examining transmission of pseudomonas aeruginosa among 76 cystic fibrosis patients in a rehabilitation center, where patients stay in close contact during prolonged periods, several clusters of patients carrying genotypically identical p. aeruginosa, as well as two clusters of 4 and 10 patients, respectively, colonized with genotypically identical achromobacter xylosoxidans strains, were discovered.	2005	15956444
a novel bacterium achromobacter xylosoxidans as a cause of liver abscess: three case reports.	achromobacter xylosoxidans is a microorganism that may cause opportunistic infections. we detected a. xylosoxidans in three of 46 patients with liver abscess. the clinicopathologic findings of the three patients were uniform with respect to a prior history of cholecystectomy, multi-lobulated 'coral-like' abscess under ct and epithelioid granulomas in the periphery of the abscess. achromobacter xylosoxidans is an unrecognized cause of liver abscess in humans.	2005	15978693
antimicrobial peptide therapeutics for cystic fibrosis.	greater than 90% of lung infections in cystic fibrosis (cf) patients are caused by pseudomonas aeruginosa, and the majority of these patients subsequently die from lung damage. current therapies are either targeted at reducing obstruction, reducing inflammation, or reducing infection. to identify potential therapeutic agents for the cf lung, 150 antimicrobial peptides consisting of three distinct structural classes were screened against mucoid and multidrug-resistant clinical isolates of p. aeru ...	2005	15980369
alcaligenes xylosoxidans bacteremia: clinical features and microbiological characteristics of isolates.	bacteremia caused by alcaligenes xylosoxidans is rare. between 1999 and 2002, 12 cases of bacteremia caused by a. xylosoxidans were diagnosed at a tertiary referral center in central taiwan. the clinical features of these patients and the antimicrobial susceptibilities and pulsed-field gel electrophoresis (pfge) pattern of their blood isolates were studied. all infections were acquired nosocomially. all of the adult patients had underlying diseases, and 10 (83%) had undergone an invasive procedu ...	2005	15986070
['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp].	gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ...	2005	15991478
cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans.	amidases are very important enzymes for industrial biocatalysis. we scored a novel amidase by screening the achromobacter xylosoxidans gene library with cephalosporin analogous amides. the gene coding for the enzyme, designated ana, was cloned, sequenced and overexpressed in escherichia coli. sequence analysis of ana showed it to be an amidase signature family member. interestingly, we noted that almost all ana homologous amidases are from human pathogens responsible for chronic lung infections. ...	2005	16002239
in vitro evaluation of efficacy of 5 methods of disinfection on mouthpieces and facemasks contaminated by strains of cystic fibrosis patients.	home-nebulizers are a potential source of bacterial infection of the respiratory tract in patients suffering from cystic fibrosis. recommendations for disinfecting this equipment are often arbitrary and sometimes contradictory.	2005	16046195
[use of 16s rrna gene sequencing for identification of "pseudomonas-like" isolates from sputum of patients with cystic fibrosis].	since nonfermenting, gram negative bacilli recovered from patients with cystic fibrosis could be misidentified with phenotypic procedures, we used partial 16s ribosomal rna gene (16s gene) sequencing to identify these "pseudomonas-like" isolates. 473 isolates were recovered from 66 patients in 2003. sequencing was used to identify 29 (from 24 patients) of the 473 isolates, showing unclear results with routine tests. pcr with specific primers was carried out to amplify a 995 bp fragment, which wa ...	2005	16081224
burkholderia and emerging pathogens in cystic fibrosis.	persons with cystic fibrosis (cf) are susceptible to respiratory tract infection with a variety of bacterial species. among these are several species in the genus burkholderia (including members of the b. cepacia complex, b. gladioli, and b. pseudomallei), stenotrophomonas maltophilia, achromobacter ( alcaligenes) xylosoxidans, certain ralstonia species, and species within the new genus pandoraea. enterobacteriaceae and several other rather unusual or novel species also can be found. many of the ...	2003	16088584
patient cohorting and infection control.	patients with cystic fibrosis (cf) have an abnormal propensity for recurrent and chronic infections of the lower respiratory tract (lrt), and the most common cause of a shortened lifespan is chronic infection with pseudomonas aeruginosa. a few other gram-negative organisms, primarily burkholderia cepacia complex have, however, emerged as serious pathogens capable of establishing chronic lrt infection. details of these and other cf pathogens can be found in the article by dr. john lipuma, burkhol ...	2003	16088586
[alcaligenes xylosoxidans--a pathogen of chronic ear infection].	we discuss a case of a 48-year old man admitted to our department with symptoms of chronic ear infection, that didn't answer to typical conservative treatment. the culture of the discharge was positive for alcaligenes xylosoxidans.	2005	16095102
characteristics of achromobacter xylosoxidans bacteremia in northern taiwan.	there were 40 cases of achromobacter xylosoxidans bacteremia during a 6-year period in a medical center in northern taiwan. males outnumbered females (72.5% vs 27.5%). common underlying diseases and conditions in these 40 bacteremic patients were malignancies (57.5%), central venous catheter implants (55%), surgery (35%), neutropenia (30%) and use of steroids (25%). recurrent bacteremia occurred in 4 patients (10%), all of whom had a central venous catheter implant which was considered the most ...	2005	16118676
tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in jammu and kashmir, india.	twelve chemolithotrophic strains were isolated from temperate orchard soil on reduced sulfur compounds as energy and electron sources and characterized on the basis of their physiological properties and ability to oxidize various reduced sulfur compounds. the new isolates could oxidize tetrathionate as well as thiosulfate, and oxidation of the latter involved conversion of thiosulfate to tetrathionate followed by its accumulation and eventual oxidation to sulfate, manifested in the production of ...	2005	16166666
a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans infection.	to report a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans.	2005	16209287
efficient conversion of itaconic acid to (s)-(+)-citramalic acid by alcaligenes xylosoxydans il142.	the effective production of (s)-(+)-citramalic acid from itaconic acid with an enantiomeric purity of more than 99.9% was successfully achieved using resting cells of a newly isolated strain, alcaligenes xylosoxydans il142. the highest conversion activity was obtained with an itaconic acid concentration of 65.0 g.l(-1). after 30 h of reaction, 68.9 g.l(-1) of (s)-(+)-citramalic acid was produced from 65.0 g.l(-1) of itaconic acid. this is equivalent to a molar yield of 93.1%. this production pro ...	2000	16232765
imipenem-resistant achromobacter xylosoxidans carrying blavim-2-containing class 1 integron.	we characterized seven isolates of imipenem-resistant achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in korea during 2001 to 2003. from the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce vim-2, oxa-30, and chromosomal ampc beta-lactamase with a pi of 8.4. they showed a similar antibiogram, which were resistant to all tested aminoglycosides as w ...	2005	16260111
outbreak of long-term intravascular catheter-related bacteremia due to achromobacter xylosoxidans subspecies xylosoxidans in a hemodialysis unit.	achromobacter xylosoxidans is a rare cause of bacteremia. over a 2-week period, a. xylosoxidans subsp. xylosoxidans was isolated from blood cultures of four hemodialysis patients with long-term intravascular catheters. a culture from one atomizer that contained diluted 2.5% chlorhexidine, which had been used to disinfect the skin, yielded a. xylosoxidans subsp. xylosoxidans. no further cases were diagnosed once the use of this atomizer was discontinued. five outbreak-related strains from the fou ...	2005	16283217
investigation of hospital-acquired infections due to achromobacter xylosoxidans in a tertiary care hospital in india.		2006	16289456
achromobacter xylosoxidans endophthalmitis diagnosed by polymerase chain reaction and gene sequencing.	to report a patient with achromobacter xylosoxidansendophthalmitis that was diagnosed using polymerase chain reaction and gene sequencing.	2005	16321892
transient performance of a two-phase partitioning bioscrubber treating a benzene-contaminated gas stream.	the dynamic performance of a prototype, two-phase partitioning bioscrubber in response to fluctuating benzene waste gas feeds has been characterized through a series of spike, step, and shutdown-restart experiments. from stable operation at a nominal loading capacity of 62 +/- 6 g/(m3 h) and removal efficiencies of over 99%, the bioscrubber was subjected to influent benzene concentration step changes and spikes as high as 10- and 20-fold, respectively. the bioscrubber responded rapidly and effec ...	2005	16323802
vim-2 metallo-beta-lactamase in achromobacter xylosoxidans in europe.		2005	16333608
production of d-aminoacylase from alcaligenes denitrificans subsp. xylosoxydans mi-4.	a bacterial strain that produces d-aminoacylase was isolated from soil and identified as alcaligenes denitrificans subsp. xylosoxydans mi-4. l-aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-aminoacylase was inducibly produced. n-acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. enzymatic resolution of n-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was appr ...	1988	16347776
high-level nickel resistance in alcaligenes xylosoxydans 31a and alcaligenes eutrophus kto2.	two new nickel-resistant strains of alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. alcaligenes xylosoxydans 31a is a heterotrophic bacterium, and alcaligenes eutrophus kto2 is an autotrophic aerobic hydrogen-oxidizing bacterium. both strains carry-among other plasmids-a megaplasmid determining resistance to 20 to 50 mm nicl(2) and 20 mm cocl(2) (when growing ...	1991	16348590
4-methylumbelliferyl-beta-n-acetylglucosaminide hydrolysis by a high-affinity enzyme, a putative marker of protozoan bacterivory.	hydrolysis of an artificial fluorogenic substrate, 4-methylumbelliferyl-beta-n-acetylglucosaminide, has been studied in a monoculture predator-prey system with either a flagellate (bodo saltans) or a ciliate (cyclidium sp.) fed upon pure bacterial culture (aeromonas hydrophila or alcaligenes xylosoxidans). aeromonas hydrophila produced a low-affinity beta-n-acetylglucosaminidase-like enzyme (k(m), >>100 mumol liter) but alcaligenes xylosoxidans did not. inoculation of both bacterial strains with ...	1993	16349049
microbiology of airway disease in a cohort of patients with cystic fibrosis.	recent reports document an increasing incidence of new gram-negative pathogens such as stenotrophomonas maltophilia and alcaligenes xylosoxidans isolated from patients with cystic fibrosis, along with an increase in common gram-negative pathogens such as pseudomonas aeruginosa and burkholderia cepacia complex. furthermore, the increase in multidrug-resistance of such organisms makes the therapeutic management of these patients more problematic. therefore, careful isolation and identification, an ...	2006	16405721
nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems.	dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ...	1995	16535048
transient performance of two-phase partitioning bioreactors treating a toluene contaminated gas stream.	two-phase partitioning bioreactors (tppbs) consist of a cell-containing aqueous phase and an immiscible organic phase that sequesters and delivers toxic substrates to cells based on equilibrium partitioning. the immiscible organic phase, which acts as a buffer for inhibitory substrate loadings, makes it possible for tppbs to handle high volatile organic compound (voc) loadings, and in this study the performance of liquid n-hexadecane and solid styrene butadiene (sb) polymer beads used as partiti ...	2006	16607657
isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards.	deleterious rhizosphere inhabiting bacteria (drb) have potential to suppress plant growth. this project focuses on the isolation of drb with potential for development as commercial products for weed control.	2006	16629995
structure solution of azurin ii from alcaligenes xylosoxidans using the laue method: possibility of studying in situ redox changes using x-rays.	we have recently demonstrated that x-rays can be used for changing the redox states of the metal centre in metalloproteins [murphy et al. (1995). j. synchrotron rad. 2, 64-69]. the possibility of using the laue method for studying the structural changes associated with such x-ray-induced reactions is explored by applying the method to the structure determination of a new azurin (hereafter referred to as azurin ii) from the denitrifying bacterium alcaligenes xylosoxidans. laue x-ray diffraction d ...	1996	16702653
fatal alcaligenes xylosoxidans infection of the liver: presenting as a liver mass after cholecystectomy.		2006	16725005
[community-acquired pneumonia and bacteraemia due to alcaligenes xylosoxidans].		2006	16752454
chronic infection with achromobacter xylosoxidans in cystic fibrosis patients; a retrospective case control study.	in cystic fibrosis (cf), chronic infection of the airways with achromobacter xylosoxidans have become more frequent. the pathogenic role of this is yet unclear.	2006	16777495
achromobacter xylosoxidans in cystic fibrosis: prevalence and clinical relevance.	achromobacter xylosoxidans is increasingly cultured in sputum from cystic fibrosis (cf) patients; nevertheless, there are few published data on the clinical impact of this infection or chronic colonisation.	2007	16793350
direct estimation of the oxygen requirements of achromobacter xylosoxidans for aerobic degradation of monoaromatic hydrocarbons (btex) in a bioscrubber.	the o2 requirements for biomass production and supplying maintenance energy demands during the degradation of both benzene and ethylbenzene by achromobacter xylosoxidans y234 were measured using a newly proposed technique involving a bioscrubber. using this approach, relevant microbial parameter estimates were directly and simultaneously obtained via linear regression of pseudo steady-state data. for benzene and ethylbenzene, the biomass yield on o2, y(x/o2), was estimated on a cell dry weight ( ...	2006	16802093
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods.	to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...	2007	16822636
coordinate synthesis of azurin i and copper nitrite reductase in alcaligenes xylosoxidans during denitrification.	the denitrifying bacterium alcaligenes xylosoxidans synthesises two azurins (az), which are termed az i and az 2. both function as effective electron donors to copper nitrite reductase (nir) in vitro. as a first step towards identifying the physiological relevance of these electron transfer proteins in the denitrification process, the gene (azua) encoding az i was characterised and its expression with respect to denitrification determined. we show that the azua gene from a. xylosoxidans is monoc ...	2006	16832626
benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation.	the performance and stability of a two-phase partitioning bioscrubber (tppb) containing 33% (vol.) n-hexadecane as an immiscible phase was investigated during 30 days of continuous gaseous benzene treatment. elimination capacities of 141 +/- 12 g/m(3) h were achieved by achromobacter xylosoxidans y234 while maintaining >99% removal throughout. a new steady-state operating strategy that limits excessive biomass production by directing substrate consumption to maintenance energy has eliminated the ...	2006	16941180
post-ercp bacteremia caused by alcaligenes xylosoxidans in a patient with pancreas cancer.	alcaligenes xylosoxidans is an aerobic, motile, oxidase and catalase positive, nonfermentative gram negative bacillus. this bacterium has been isolated from intestine of humans and from various hospital or environmental water sources. a.xylosoxidans is both waterborne and results from the poor-hygienic conditions healthcare workers are in. in this case report, the bacteremia which appeared in a patient with pancreas cancer after ercp was described.	2006	16945159
fluorescence in situ hybridization for rapid identification of achromobacter xylosoxidans and alcaligenes faecalis recovered from cystic fibrosis patients.	achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (cf) patients, but identification with biochemical tests is unreliable. we describe fluorescence in situ hybridization assays for the rapid identification of achromobacter xylosoxidans and alcaligenes faecalis. both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from cf patients.	2006	16954289
ciprofloxacin-resistant gram-negative bacilli in the fecal microflora of children.	the extent to which antibiotic-resistant bacteria are excreted by humans who have not been exposed to antibiotics is not known. children, who rarely receive fluoroquinolones, provide opportunities to assess the frequency of fecal excretion by fluoroquinolone-naïve hosts of fluoroquinolone-resistant gram-negative bacilli. fresh nondiarrheal stools from children were processed by screening them on agar containing ciprofloxacin to recover ciprofloxacin-resistant gram-negative bacilli. resistant iso ...	2006	17005812
construction of a leftover bath water model for microbial testing.	in this study, in order to construct a model of leftover bath water, we analyzed one hundred samples of used bath water samples which were provided by twenty-eight volunteer families. it appeared that the number of detected bacteria from such bath water was correlated closely with the number of bathers. moreover, the ph, acidity, chemical oxygen demand (cod), ion, protein content of the leftover bath water were measured. the number of bathers had no connection with the ph, acidity, cod, and ion ...	2006	17017128
intersite structural rearrangement of the blue copper site induced by substrate binding: spectroscopic studies of a copper-containing nitrite reductase from alcaligenes xylosoxidans ncimb 11015.	a copper-containing nitrite reductase from alcaligenes xylosoxidans ncimb 11015 has its own unique blue or type 1 copper protein resonance raman spectrum in the usual cu-s(cys) stretching region, nu(cu-s(cys)), with a pair of strong peaks at 412 and 420 cm(-1) and a weak peak at 364 cm(-1). the predominantly nu(cu-s(cys)) raman bands at 412, 420, and 364 cm(-1) of the type 1 copper site all shifted to higher frequencies upon binding of nitrite to the type 2 copper site, and the resonance raman d ...	2006	17029353
analysis of the composition of bacterial communities in oil reservoirs from a southern offshore brazilian basin.	the aim of this study was to characterize and compare the bacterial community structure of two distinct oil samples from a petroleum field in brazil by using both molecular, based on the construction of 16s rrna gene libraries, and cultivation methods. statistical comparisons of libraries based on amplified ribosomal dna restriction analysis (ardra) data revealed no significant differences between the communities recovered in the non-biodegraded (nbd) and highly biodegraded oils (hbd). blastn an ...	2007	17072536
bilateral simultaneous achromobacter xylosoxidans keratitis following penetrating keratoplasty.	we report a case of severe bilateral simultaneous achromobacter xylosoxidans keratitis following penetrating keratoplasty (pkp) in a patient with graft-versus-host disease. the predisposing factors, clinical features, treatment options, and final visual outcome are reviewed. to our knowledge, this is the first report of bilateral a xylosoxidans keratitis following pkp.	2006	17138001
molecular basis for nitric oxide dynamics and affinity with alcaligenes xylosoxidans cytochrome c.	the bacterial heme protein cytochrome ć from alcaligenes xylosoxidans (axcp) reacts with nitric oxide (no) to form a 5-coordinate ferrous nitrosyl heme complex. the crystal structure of ferrous nitrosyl axcp has previously revealed that no is bound in an unprecedented manner on the proximal side of the heme. to understand how the protein structure of axcp controls no dynamics, we performed absorption and raman time-resolved studies at the heme level as well as a molecular computational dynamics ...	2007	17158883
infection of a total hip prosthesis in a dog caused by achromobacter(alcaligenes) xylosoxidans.	a four-year-old male, neutered labrador retriever was presented with progressive left hindlimb lameness 10 months following total hip replacement. radiography revealed changes consistent with infection and culture of joint fluid from the left coxofemoral joint revealed achromobacter (alcaligenes) xylosoxidans. the prosthesis was removed. culture of the acetabular cup confirmed achromobacter xylosoxidans. achromobacter xylosoxidans is an uncommon but serious cause of nosocomial epidemics in hospi ...	2006	17201829
[isolation, identification and degradation characteristics of bisphenol a degrading strain].	a bisphenol a(bpa) degrading strain b-16 was isolated from compost leachate of municipal solid waste. according to the morphological characteristics, physiological and biochemical, and sequence analysis of 16s rdna, the strain was identified preliminarily as achromobacter xylosoxidans. by tests in shaking flasks, the effects of the conditions of growth was studied, and it was determined that the optimum conditions were inoculum amount 0.6%, ph 7.0, and 30 degrees c. under these conditions, when ...	2006	17256617
veb-1 in achromobacter xylosoxidans from cystic fibrosis patient, france.		2006	17283625
aerobic degradation of bisphenol a by achromobacter xylosoxidans strain b-16 isolated from compost leachate of municipal solid waste.	a novel bacterium designated strain b-16 was isolated from the compost leachate of the municipal solid waste (msw) in a laboratory reactor. this strain was identified as a gram-negative bacterium, achromobacter xylosoxidans that could grow on bisphenol a (bpa, a representative endocrine disruptor) as a sole carbon source under aerobic condition. bpa-degrading characteristics of strain b-16 were investigated in liquid cultures. the results show that bpa degradation was influenced by several facto ...	2007	17291567
emerging cystic fibrosis pathogens: incidence and antimicrobial resistance.	we examined the frequency of isolation and the antimicrobial resistance of burkholderia cepacia complex, stenotrophomonas maltophilia and achromobacter xylosoxidans in cystic fibrosis patients from 2000 to 2004. strains susceptibility to tobramycin, piperacillin/tazobactam, imipenem, gentamicin, ciprofloxacin and ceftazidime was determined by disc diffusion assay. b. cepacia complex showed a very high resistance also to ciprofloxacin reaching 100% in 2004. s. maltophilia and a. xvylosoxidans sho ...	2007	17319602
salvage treatment of pneumonia and initial treatment of tracheobronchitis caused by multidrug-resistant gram-negative bacilli with inhaled polymyxin b.	systemic colistin has shown efficacy against multidrug-resistant pseudomonas aeruginosa and acinetobacter spp., but it has presented poor results in pneumonia. aerosolized polymyxin in cystic fibrosis patients has had good results. in this study, inhaled polymyxin b was used to treat respiratory infections by multidrug-resistant gram-negative bacilli (mr-gnbs). nineteen patients were treated with inhaled polymyxin b: 14 pneumonia, most of which had previously failed treatment with intravenous po ...	2007	17350201
culturable bacteria present in the fluid of the hooded-pitcher plant sarracenia minor based on 16s rdna gene sequence data.	the culturable microbial community within the pitcher fluid of 93 sarracenia minor carnivorous plants was examined over a 2-year study. many aspects of the plant/bacterial/insect interaction within the pitcher fluid are minimally understood because the bacterial taxa present in these pitchers have not been identified. thirteen isolates were characterized by 16s rdna sequencing and subsequent phylogenetic analysis. the proteobacteria were the most abundant taxa and included representatives from s ...	2007	17380356
presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser.	reported here are the microbiological and epidemiological details of a presumed outbreak of aerobic gram-negative bacilli infections affecting 19 hematological patients, which was traced to contaminated disinfectant. over a 5-month period, the following organisms were isolated from the blood cultures of 19 neutropenic patients: pseudomonas fluorescens (n = 13), achromobacter xylosoxidans (n = 12), comamonas testosteroni (n = 2) or stenotrophomonas maltophilia (n = 1). the affected patients were ...	2007	17393202
[isolation of achromobacter xylosoxidans ns12 and degradation of nitrophenols].	a nitrophenols-degrading bacterium, strain ns12, was isolated from a mangrove sediment by enrichment culture under aerobic conditions. based on the analysis of 16s rdna gene sequence the isolate was identified as achromobacter xylosoxidans strain ns12 was able to metabolize both o-nitrophenol (onp) and p-nitrophenol (pnp) as the sole source of carbon, energy and nitrogen. however, this strain was not able to use 3-nitrophenol (mnp) as the only source of carbon energy and nitrogen for growth. the ...	2007	17489210
the structure of the met144leu mutant of copper nitrite reductase from alcaligenes xylosoxidans provides the first glimpse of a protein-protein complex with azurin ii.	cu-containing nitrite reductases (nirs) perform the reduction of nitrite to no via an ordered mechanism in which the delivery of a proton and an electron to the catalytic type 2 cu site is highly orchestrated. electron transfer from a redox partner protein, azurin or pseudoazurin, to the type 1 cu site is assumed to occur through the formation of a protein-protein complex. we report here a new crystal form in space group p2(1)2(1)2(1) of the met144leu mutant of nir from alcaligenes xylosoxidans ...	2007	17503096
evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing.	ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ...	2007	17507509
outbreak of achromobacter xylosoxidans pseudobacteremia in a neonatal care unit related to contaminated chlorhexidine solution.		2007	17516103
emerging and unusual gram-negative infections in cystic fibrosis.	people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ...	2007	17562501
patient risk of contact with respiratory pathogens from inanimate surfaces in a cystic fibrosis outpatient clinic. a prospective study over a four-year period.	acquisition of respiratory pathogens such as pseudomonas aeruginosa (pa) is associated with increased morbidity and mortality in cystic fibrosis (cf). research on the prevalence of these pathogens on environmental surfaces of a cf center is scanty, and so far no study has determined what risk cf patients have of coming in contact with them during their visits to the cf center. this study is aimed at assessing the prevalence of some respiratory pathogens in samples taken systematically during a 4 ...	2007	17639586
alcaligenes xylosoxidans conjunctivitis.	to report a case of chronic unilateral conjunctivitis caused by alcaligenes xylosoxidans.	2007	17667625
survival of lung transplant patients with cystic fibrosis harboring panresistant bacteria other than burkholderia cepacia, compared with patients harboring sensitive bacteria.	the impact of panresistant bacteria, other than burkholderia cepacia, on the survival after lung transplantation in patients with cystic fibrosis (cf) remains controversial.	2007	17692788
alcaligenes xylosoxidans osteomyelitis without trauma in a patient with good's syndrome.		2007	17693242
successful treatment of alcaligenes xylosoxidans in automated peritoneal dialysis-related peritonitis.		2007	17704455
genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases.	recently, the structure of a cu-containing nitrite reductase (nir) from hyphomicrobium denitrificans (hdnir) has been reported, establishing the existence of a new family of cu-nir where an additional type 1 cu (t1cu) containing cupredoxin domain is located at the n-terminus (nojiri et al. in proc. natl. acad. sci. usa 104:4315-4320, 2007). hdnir retains the well-characterised coupled t1cu-type 2 cu (t2cu) core, where the t2cu catalytic site is also built utilising ligands from neighbouring mono ...	2007	17712582
urinary tract infection due to achromobacter xylosoxidans: report of 9 cases.	urinary tract infection (uti) due to achromobacter xylosoxidans is rare. the aims were to know the frequency and clinical characteristics of this infection in our area. we performed a retrospective analysis of 9 patients with uti caused by this organism diagnosed over a period of 13 y. the mean age was 63.1 y. all patients had underlying diseases or urological abnormalities. the most frequent underlying diseases were solid or hematological malignancies (3 cases). seven patients (77.7%) had urolo ...	2008	17852927
a case of native valve infective endocarditis caused by alcaligenes xylosoxidans.		2007	17868590
iatrogenic hemolytic anemia and endocarditis in new zealand white rabbits secondary to achromobacter xylosoxidans infection.	during a 3-mo period, 9 of the 15 new zealand white rabbits used in a heart failure study developed a hemolytic anemia. the heart failure model involved the creation of an aortic insufficiency (ai) followed 2 to 6 wk later by the creation of an aortic stenosis (as). none of the 9 animals that developed hemolytic anemia responded to medical management, and 6 of the 9 were euthanized for humane concerns. necropsies and blood cultures were performed on all anemic animals; 7 of these cultures yielde ...	2007	17994675
nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pa81 from achromobacter xylosoxidans a8.	the complete 98,192bp nucleotide sequence was determined for plasmid pa81, which is harbored by the haloaromatic acid-degrading bacterium achromobacter xylosoxidans a8. the majority of the 103 open reading frames identified on pa81 could be categorized as either "backbone" genes, genes encoding (halo)aromatic compound degradation, or heavy metal resistance determinants. the backbone genes controlled conjugative transfer, replication and plasmid stability, and were well conserved with other incp1 ...	2008	18249097
modulation of no binding to cytochrome c' by distal and proximal haem pocket residues.	we have cloned and expressed the cycp gene encoding cytochrome c' from alcaligenes xylosoxidans and generated mutations in arg-124 and phe-59, residues close to the haem, to probe their involvement in modulating the unusual spin-state equilibrium of the haem fe and the unique proximal mode of binding of no to form a stable five-coordinate adduct. arg-124 is located in the proximal pocket of the haem and forms a hydrogen bond to the stable five-coordinated bound no. phe-59 provides steric hindran ...	2008	18259785
[pseudomonas and alcaligenes--pathogens of the chronic ear infection in clinical cases at the otolaryngology department of the university hospital in krakow].	otitis media chronica is the most common disorders encountered in otolaryngology. the great multitude of different bacteria which may couse otitis media constitutes the need for bacteriological diagnostics, especially in cases where classical conservative treatement of the inflammatory process fails.	2007	18260228
meningitis caused by alcaligenes xylosoxidans in a patient with hiv/aids.	the purpose of the present work was to inform about the first case of meningitis associated to the bacteria alcaligenes xylosoxidans in a patient with hiv/aids. the patient was a 46-year-old male, with the antecedent of have been diagnosed for hiv/ aids, who attended in the hospital universitario de colima, mexico, with fever, shock and meningismus. the study of the cerebrospinal fluid showed pleocytosis, elevated protein levels and hypoglycorrhachia. the culture yielded the presence of alcalige ...	2007	18327475
inoculation of plant growth promoting bacterium achromobacter xylosoxidans strain ax10 for the improvement of copper phytoextraction by brassica juncea.	in this study, a copper-resistant plant growth promoting bacterial (pgpb) strain ax10 was isolated from a cu mine soil to assess its plant growth promotion and copper uptake in brassica juncea. the strain ax10 tolerated concentrations up to 600 mg cul(-1) on a luria-bertani (lb) agar medium and utilized 1-aminocyclopropane-1-carboxylic acid (acc) as a sole n source in df salts minimal medium. the strain ax10 was characterized as achromobacter xylosoxidans based on its 16s rdna sequence homology ...	2009	18329785
achromobacter xylosoxidans subsp. xylosoxidans prosthetic aortic valve infective endocarditis and aortic root abscesses.	we report a case of prosthetic valve infective endocarditis and aortic root abscesses caused by achromobacter xylosoxidans subsp. xylosoxidans. the patient was an intravenous drug user and had injected amphetamines using 'duck pond water' as a diluent. after surgical intervention and 6 weeks of intravenous meropenem therapy, the patient made an uneventful recovery.	2008	18349376
characterization of a naturally occurring class d beta-lactamase from achromobacter xylosoxidans.	a chromosomally encoded class d beta-lactamase, oxa-114, was characterized from achromobacter xylosoxidans strain cip69598. beta-lactamase oxa-114 shared 56% amino acid identity with the naturally occurring class d beta-lactamase of burkholderia cenocepacia and 42% identity with the acquired oxacillinases oxa-9 and oxa-18. oxa-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. pcr and sequencing revealed that bla(oxa-114)-like genes were identified ...	2008	18362192
[(3)h]adenine is a suitable radioligand for the labeling of g protein-coupled adenine receptors but shows high affinity to bacterial contaminations in buffer solutions.	[(3)h]adenine has previously been used to label the newly discovered g protein-coupled murine adenine receptors. recent reports have questioned the suitability of [(3)h]adenine for adenine receptor binding studies because of curious results, e.g. high specific binding even in the absence of mammalian protein. in this study, we showed that specific [(3)h]adenine binding to various mammalian membrane preparations increased linearly with protein concentration. furthermore, we found that tris-buffer ...	2007	18404448
postoperative alcaligenes xylosoxidans endophthalmitis: report of two cases.	we report two cases of postoperative alcaligenes xylosoxidans endophthalmitis. a 78-year-old woman in good general health developed a. xylosoxidans endophthalmitis one month after an uncomplicated phacoemulsification procedure with posterior chamber intraocular lens. it was performed on her left eye, at another institution. removal of the intraocular lens and capsule was performed because of recurrent inflammation after vitrectomy and intravitreal antibiotic injections. her son, a 55-year-old ma ...	2008	18408852