Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| relativein vitro effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown in oocytes of indian major carps,labeo rohita, cirrhinus mrigala andcatla catla. | the relative effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown (gvbd) was investigatedin vitro using folliculated oocytes of three carps,labeo rohita, cirrhinus mrigala, andcatla catla. in all three species progesterone and 17α-hydroxyprogesterone could induce gvbd but relatively 17α,20β-dihydroxyprogesterone was consistently found to be the most potent maturation-inducing steroid. both estradiol-17β and testoste ... | 1989 | 24221598 |
| establishment of a leukocyte cell line derived from peritoneal macrophages of fish, labeo rohita (hamilton, 1822). | a continuous leukocyte cell line with phagocytic activity was established from peritoneal macrophages of rohu, labeo rohita (lrpm). lrpm was initiated from adherent mononuclear leukocytes isolated from peritoneal cavity of rohu, without use of any growth factors or feeder cells. these cells exhibited maximum growth at 30 °c in l-15 medium containing 20 % foetal bovine serum, and has been subcultured for more than 60 passages till date. the cells showed 85 % viability after 6 months of storage in ... | 2015 | 24248274 |
| outer membrane protein assembly factor yaet (omp85) and groel proteins of edwardsiella tarda are immunogenic antigens for labeo rohita (hamilton). | 2014 | 24320972 | |
| age and growth in the indian major carp labeo rohita (cypriniformes: cyprinidae) from tropical rivers of ganga basin, india. | labeo rohita is a member of the indian major carp species and originally an inhabitant of the ganga river network in india. it is among the top ten aquaculture species of the world. since there is a lack of information on the growth pattern of the wild populations of this species, this study aimed at evaluating the pattern of age and growth, to support the development of effective management plans. a total of 1082 samples of l. rohita were obtained from may 2009 to july 2012 in six drainages of ... | 2013 | 24432546 |
| molecular characterization and expression of type-i interferon gene in labeo rohita. | genes coding for type-i interferon (i-ifn) has been cloned from labeo rohita, a commercially important and widely cultured fish in india and south east asia. in the present study, full-length gene of i-ifn was amplified and sequenced. the sequence analysis revealed that i-ifn consists of 1,786 bp genomic sequence with four introns and five exons and an orf of 546 bp encoding for a putative protein of 181 amino acids. the mature protein has a molecular weight of 18.97 kda and consists of 158 amin ... | 2014 | 24449367 |
| post-harvest loss of farm raised indian and chinese major carps in the distribution channel from mymensingh to rangpur of bangladesh. | post-harvest loss of catla (catla catla), rohu (labeo rohita), mrigal (cirrhinus mrigala), silver carp (hypophthalmichthys molitrix), grass carp (ctenopharyngodon idella) and sarpunti (puntius sarana) in a single distribution chain from harvest in mymensingh to retail sale rangpur town were determined, in order to obtain information on quality deterioration and existing handling and icing conditions so that suggestions for improving such practices can be made. quality defect points of the fish a ... | 2013 | 24494525 |
| study on antimicrobial potential of neem oil nanoemulsion against pseudomonas aeruginosa infection in labeo rohita. | presence of several biochemical constituents in neem makes it an efficient antimicrobial agent for pathogenic diseases. the current investigation was aimed to assess the therapeutic potential of neem nanoemulsion as a control measure for pseudomonas aeruginosa infection in freshwater fish labeo rohita. the median lethal concentration (lc50) for the neem oil and neem nanoemulsion was 73.9 and 160.3 mg/l, respectively. the biomarker enzymes of treated fish tissues showed a significant difference i ... | 2016 | 24502533 |
| in vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of catla catla and gill cell line of labeo rohita. | silver nanoparticles (ag-nps) are used in commercial products for their antimicrobial properties. the ag-nps in some of these products are likely to reach the aquatic environment, thereby posing a health concern for humans and aquatic species. the silver nanoparticles were synthesized and characterized using, uv-vis spectra, dynamic light scattering (dls) and transmission electron microscopy (tem) analysis. acute toxicity tests on fish were conducted by exposing catla catla and labeo rohita for ... | 2014 | 24524868 |
| gene structure and identification of minimal promoter of pou2 expressed in spermatogonial cells of rohu carp, labeo rohita. | mammalian pou5f1 is a known transcriptional regulator involving maintenance of embryonic and spermatogonial stem cells. little is known about teleost pou2, an ortholog of mammalian pou5f1. evidences of discrepancy in expression pattern between fish species were documented. to better understand, we have cloned and characterized pou2 gene of farmed rohu carp, labeo rohita. it contained five exons with an open reading frame of 1419 bp long, translatable to 472 aa. a bipartite dna binding domain ter ... | 2014 | 24566687 |
| comparative organoleptic quality of indian major carps collected from pond and reservoir. | an attempt was made to distinguish the possible organoleptic differences between the pond reared and wild fish species of reservoirs and correlate these with the physicochemical characteristics of water bodies. the texture was found to firmer in wild varieties of fishes than pond reared fish, with best in catla catla followed by labeo rohita and cirrhinus mrigala. all the cultured fishes had lighter colour than wild fish species which were darker in appearance. comparatively, higher dissolved ox ... | 2013 | 24640254 |
| assessment of genotoxic and mutagenic potential of hexavalent chromium in the freshwater fish labeo rohita (hamilton, 1822). | the present study was undertaken to investigate the genotoxicity and mutagenicity of sublethal concentrations of hexavalent chromium (potassium dichromate) in the indian major carp, labeo rohita. the 96 h lc50 value of potassium dichromate estimated was 118 mg l(-1) by probit analysis using spss (version 16.0) software. based on 96 h lc50 value, three sublethal test concentrations of potassium dichromate (29.5, 59.0 and 88.5 mg l(-)(1)) were selected and specimens were exposed in vivo to these t ... | 2015 | 24649973 |
| effect of dactylogyrus catlaius (jain 1961) infection in labeo rohita (hamilton 1822): innate immune responses and expression profile of some immune related genes. | the monogenean ectoparasite, dactylogyrus sp. is a major pathogen in freshwater aquaculture. the immune responses in parasitized fish were analyzed by quantitation of innate immune factors (natural agglutinin level, haemolysin titre, antiprotease, lysozyme and myeloperoxidase activities) in serum and immune-relevant gene expression in gill and anterior kidney. the antiprotease activity and natural agglutinin level were found to be significantly higher and lysozyme activity was significantly lowe ... | 2014 | 24669670 |
| molecular cloning, characterization and functional assessment of the myosin light polypeptide chain 2 (mylz2) promoter of farmed carp, labeo rohita. | we cloned the 5'-flanking region (1.2 kb) of a muscle-specific gene, encoding myosin light chain 2 polypeptide (mylz2) of a farmed carp, labeo rohita (rohu). sequence analysis using transfac-database search identified the consensus cis acting regulatory elements of tata-box and e (canntg)-box, including the monocyte enhancer factor 2 motif, implying that it is likely to be a functional promoter. the proximal promoter (~620 bp) was highly homologous with that of danio rerio (zebrafish) as compare ... | 2014 | 24740361 |
| effect of ice storage on the functional properties of proteins from a few species of fresh water fish (indian major carps) with special emphasis on gel forming ability. | in the present study the effect of ice storage on physico-chemical and functional properties of proteins from indian major carps with special emphasis on gel forming ability have been assessed for a period of 22 days. the solubility profile of proteins in high ionic strength buffer and calcium adenosine triphosphatase (atpase) enzyme activity reduced significantly (p < 0.05), while that of total volatile base nitrogen (tvb-n) increased significantly (p < 0.05) at the end of 22 days of ice storag ... | 2014 | 24741158 |
| evaluation of trace metal content by icp-ms using closed vessel microwave digestion in fresh water fish. | the objective of the present study was to investigate trace metal levels of different varieties of fresh water fish using inductively coupled plasma mass spectrophotometer after microwave digestion (md-icpms). fish samples were collected from the outlets of twin cities of hyderabad and secunderabad. the trace metal content in different varieties of analyzed fish were ranged from 0.24 to 1.68 mg/kg for chromium in cyprinus carpio and masto symbollon, 0.20 to 7.52 mg/kg for manganese in labeo rohi ... | 2014 | 24744789 |
| cross-priming of microsatellite loci in subfamily cyprininae (family cyprinidae): their utility in finding markers for population genetic analysis in three indian major carps. | this study is aimed to identify polymorphic microsatellite markers and establish their potential for population genetics studies in three carp (family cyprinidae; subfamily cyprininae) species, labeo rohita, catla catla and cirrhinus mrigala through use of cyprinid primers. these species have high commercial value and knowledge of genetic variation is important for management of farmed and wild populations. we tested 108 microsatellite primers from 11 species belonging to three different cyprini ... | 2014 | 24792330 |
| immune responses and protective efficacy of recombinant outer membrane protein r (rompr)-based vaccine of aeromonas hydrophila with a modified adjuvant formulation in rohu (labeo rohita). | despite the importance and success of developing a candidate vaccine against aeromonas hydrophila infection in fish, little is known about the molecular mechanisms of the vaccine-induced immunoprotection in indian major carp, labeo rohita, primarily due to lack of information on most of the immune related genes of the species. in this study, a novel candidate antigen recombinant outer membrane protein r (rompr) of a. hydrophila was evaluated as a vaccine candidate along with a modified adjuvant ... | 2014 | 24937805 |
| innate immune response of indian major carp, labeo rohita infected with oomycete pathogen aphanomyces invadans. | the fish pathogenic oomycete aphanomyces invadans is the causative agent of epizootic ulcerative syndrome (eus), a fish disease of international significance and reportable to the world organisation for animal health. in spite of the current and potential impact of a. invadans infection on fisheries and aquaculture sectors of the world, very little is known about the host-a. invadans interactions. in the present study, following experimental infection with a. invadans in one of the indian major ... | 2014 | 24945572 |
| gnih and gnrh expressions in the central nervous system and pituitary of indian major carp, labeo rohita during ontogeny: an immunocytochemical study. | gonadotropin-releasing hormone (gnrh) is the major hypothalamic neuropeptide stimulating gonadotropin secretion in vertebrates. in 2000, gonadotropin-inhibitory hormone (gnih) was discovered as a hypothalamic neuropeptide that inhibits gonadotropin secretion in birds. subsequent studies have shown that gnih is present in the brain of other vertebrates. we show for the first time gnih immunoreactivity in the central nervous system and pituitary during development of indian major carp, labeo rohit ... | 2015 | 24955881 |
| beneficial effects of dietary probiotics mixture on hemato-immunology and cell apoptosis of labeo rohita fingerlings reared at higher water temperatures. | probiotics play an important role in growth increment, immune enhancement and stress mitigation in fish. increasing temperature is a major concern in present aquaculture practices as it markedly deteriorates the health condition and reduces the growth in fish. in order to explore the possibilities of using probiotics as a counter measure for temperature associated problems, a 30 days feeding trial was conducted to study the hemato-immunological and apoptosis response of labeo rohita (8.3±0.4 g) ... | 2014 | 24979660 |
| a linkage map of transcribed single nucleotide polymorphisms in rohu (labeo rohita) and qtl associated with resistance to aeromonas hydrophila. | production of carp dominates world aquaculture. more than 1.1 million tonnes of rohu carp, labeo rohita (hamilton), were produced in 2010. aeromonas hydrophila is a bacterial pathogen causing aeromoniasis in rohu, and is a major problem for carp production worldwide. there is a need to better understand the genetic mechanisms affecting resistance to this disease, and to develop tools that can be used with selective breeding to improve resistance. here we use a 6 k snp array to genotype 21 full-s ... | 2014 | 24984705 |
| identification of promoter within the first intron of plzf gene expressed in carp spermatogonial stem cells. | promyelocytic leukemia zinc finger (plzf), a transcriptional repressor, is involved in survival and maintenance of pluripotent stem cells including embryonic and spermatogonial stem cells in mammals. its cdna was characterized and expression in proliferating spermatogonial stem cells of rohu (labeo rohita), a farmed carp, was documented. in teleost, the information on its promoter activity is lacking. here, we have isolated, sequenced and performed the first characterization of regulatory elemen ... | 2014 | 24990695 |
| first evidence of comparative responses of toll-like receptor 22 (tlr22) to relatively resistant and susceptible indian farmed carps to argulus siamensis infection. | toll-like receptor 22 (tlr22) is present in teleost but not in mammals. among indian farmed carps, catla catla is relatively more resistant than labeo rohita to argulus siamensis lice infection. tlr22 is believed to be associated with innate immunity against ectoparasite infection. to investigate the tlr22 mediated immunity against argulosis, we have cloned and characterized tlr22 genes of l. rohita (rtlr22) and c. catla (ctlr22). the full-length cdnas of rtlr22 and ctlr22 contained an open read ... | 2014 | 24998226 |
| expression analysis of heat shock protein genes during aeromonas hydrophila infection in rohu, labeo rohita, with special reference to molecular characterization of grp78. | heat shock protein (hsp) genes are stress-related genes that activate the host immune system during infection. hsp genes expression in fish, studied during bacterial infections, is mostly confined to hsp70 and hsp90. the present study is an expression analysis of seven hsp genes: apg2, hsp90, hsp70, glucose-regulated protein 78 (grp78), heat shock cognate 70 (hsc70), grp75, and hsp30 during aeromonas hydrophila infection in rohu, labeo rohita. forty-eight rohu juveniles were challenged with 3 × ... | 2014 | 25037476 |
| molecular cloning, sequencing and tissue-level expression of complement c3 of labeo rohita (hamilton, 1822). | complement component c3 plays a central role in all known complement activation pathways. in the present study, we cloned, sequenced and analyzed the full-length cdna sequence of labeo rohita complement c3 (lrc3). the expression pattern of complement c3 mrna in different tissues of healthy rohu and after challenge with aeromonas hydrophila were evaluated using real-time pcr. the lrc3 cdna sequence of rohu comprised of 5081 bp encoding a predicted protein of 1645 amino acids. the deduced amino ac ... | 2014 | 25038278 |
| toll-like receptor 22 in labeo rohita: molecular cloning, characterization, 3d modeling, and expression analysis following ligands stimulation and bacterial infection. | toll-like receptors (tlrs) are a class of innate immune receptors that sense pathogens or their molecular signatures and activate signaling cascades to induce a quick and non-specific immune response in the host. among various types of tlrs, tlr22 is exclusively present in teleosts and amphibians and is expected to play the distinctive role in innate immunity. this report describes molecular cloning, three-dimensional (3d) modeling, and expression analysis of tlr22 in rohu (labeo rohita), the mo ... | 2014 | 25064133 |
| analysis of immune-related ests and differential expression analysis of few important genes in lines of rohu (labeo rohita) selected for resistance and susceptibility to aeromonas hydrophila infection. | a total of 137,629 contigs generated via de novo transcriptome assembly from resistant and susceptible lines of rohu (first generation) raised against aeromoniasis were further analyzed in terms of defence-related genes. out of 1,939 contigs showing homology to genes involved in immune processes, 1,866 were further categorised into different functional subgroups. comparative analysis revealed five genes for the first time in any carp species out of which apolipoprotein h, septin 4 isoform 3 and ... | 2014 | 25081649 |
| a comparative study on the physical, chemical and functional properties of carp skin and mammalian gelatins. | carp species forms the bulk of the aquaculture production in india and studies have shown that the filleting waste of these species, particularly skin can be a good source of gelatin. this study is a comparison of the gelatins from these unexploited sources with that of mammalian gelatins to get a better understanding of their physicochemical and functional properties with respect to mammalian gelatins. the study showed that mammalian gelatins had significantly superior physical properties viz., ... | 2014 | 25190867 |
| isolation and characterization of head kidney derived macrophages of labeo rohita. | macrophages play a significant role in non-specific defense mechanisms of all vertebrates against pathogens. one critical element in the area of fish immunology is the unavailability of in-vitro model of immune cells. therefore, it is essential to develop methods for harvesting and culture of macrophages for assessing innate immune functions of rohu, labeo rohita, an important culture fish of india. head kidney leukocytes from were isolated by density gradient sedimentation, so as to exclude oth ... | 2014 | 25204072 |
| role of hsp70 in cytoplasm protection against thermal stress in rohu, labeo rohita. | to understand the function of hsp70 of labeo rohita (lrhsp70) in cellular protection, lrhsp70 orf cdna was inserted into the plasmid of pet-32a(+) or pegfp-l1. then, the recombinant plasmids were transformed or transfected into escherichia coli cells, mouse myeloma cells (mpc-11) or fish hepatoma cells (plhc-1). western blot results revealed that lrhsp70 was expressed in e. coli cells and molecular weight was estimated to be 70 kda. in cells, lrhsp70 was over-expressed following thermal or cold ... | 2014 | 25240978 |
| characterization of bacillus spp. from the gastrointestinal tract of labeo rohita--towards to identify novel probiotics against fish pathogens. | the aim of the present study is to screen and characterize endogenous microbiota bacillus spp. from the gastrointestinal (gi) tract of labeo rohita in order to evaluate their probiotic attributes. a total of 74 isolates from the gi of l. rohita were evaluated for their antimicrobial properties by agar well-diffusion method against fish pathogens. based on the better antibacterial features, three isolates (kadr1, kadr3, and kadr4) were selected for further delineation. the three selected isolates ... | 2015 | 25274116 |
| the complete mitochondrial genome sequence of cirrhinus mrigala (♀) × labeo rohita (♂). | we cloned and sequenced the complete mitochondrial dna (mtdna) of cirrhinus mrigala (♀) × labeo rohita (♂) by pcr-based method. the total of the mtdna was 16,595 bp. it consisted of 13 protein-coding genes, 22 trnas, 2 rrna genes, and one putative control region (d-loop region). the overall base composition on strand was as follows a: 32.09%, g: 15.51%, c: 27.95%, t: 24.45%, and the a + t content 56.54%. all the protein-coding genes initiated by typical atg codon, and ten genes ended with the co ... | 2016 | 25350736 |
| genomic cloning and sequence analysis of interleukin-10 from labeo rohita. | interleukin-10 (il-10) is a pleiotropic cytokine and plays an important role in inflammation, immunoregulation and the pathogenesis of various diseases. therefore, it is our interest to isolate, clone, sequence and characterize il-10 gene from the fish labeo rohita (lr). the gene was amplified using genomic dna isolated from head kidney with primers designed on conserved sequence homologues of fishes belonging to cyprinidae family. the glril-10 is 1467 nucleotides long with five exons and four i ... | 2014 | 25489171 |
| mercury contamination in some marine biota species from khuzestan shore, persian gulf. | in this study, concentrations of mercury (hg) were analyzed in some marine biota species (fish, shrimp, and crab) from khuzestan shore, north part of the persian gulf. it was also our intention to evaluate potential risks to human health associated with seafood consumption. the results indicated that concentrations of hg in the fish and crustacean were different among the species and tissues. liver in fish and hepatopancreas in crustacean exhibited higher hg concentration than the other tissues. ... | 2016 | 25500758 |
| molecular characterization of interleukin 15 mrna from rohu, labeo rohita (hamilton): its prominent role during parasitic infection as indicated from infection studies. | interleukin 15 (il-15) is an important cytokine of fish immune system. sequence characterization of il-15 from rohu, labeo rohita revealed a mrna sequence of 1064 bp with coding sequence of 567 bp and signal peptide of 16 amino acids. there are four characteristic sequence features viz., presence of four out-of-frame aug initiation codons, four highly conserved cysteine residues, constitutive expression in all tissues and evolutionary similarity. the ontogeny study revealed maternal transfer of ... | 2015 | 25514374 |
| myxozoan infections of the three indian major carps in fish ponds around meerut, up, india, with descriptions of three new species, myxobolus basuhaldari sp. n., m. kalavatiae sp. n. and m. meerutensis sp. n., and the redescription of m. catlae and m. bhadrensis. | new myxosporean species are described from indian fishes cultured in pond farms of meerut, uttar pradesh (up) state. based upon plasmodia found in the indian major carps (catla catla, cirrhinus cirrhosus, labeo rohita and their hybrids), three new myxobolus spp., myxobolus basuhaldari sp. n., myxobolus kalavatiae sp. n. and myxobolus meerutensis sp. n., are described, and two species, myxobolus catlae and myxobolus bhadrensis, are redescribed. plasmodia of m. basuhaldari sp. n., m. kalavatiae sp ... | 2015 | 25585606 |
| examining pyrethroids, carbamates and neonicotenoids in fish, water and sediments from the indus river for potential health risks. | this 3 × 3 factorial study assessed pyrethroids, carbamates and neonicotenoids groups of pesticides in replicated samples of three fish species from low (s1, reference), medium (s2) and heavy (s3) polluted sites receiving agricultural run-offs around the indus river. water and sediment samples from the same sites were also analysed for these pesticides by using high-performance liquid chromatography. out of nine investigated pesticides, only three pesticides (deltamethrin, carbofuran and cyperme ... | 2015 | 25632902 |
| in vitro evaluation of probiotic properties of lactic acid bacteria from the gut of labeo rohita and catla catla. | we report the evaluation of probiotic properties of potent lactic acid bacteria (lab) from the gut of freshwater fishes, labeo rohita and catla catla, for eventually developing probiotic strains for the prevention of bacterial infections in aquaculture and food preservation. five different lab strains were isolated and characterized for their probiotic properties. based on physiological, morphological and biochemical characteristics, three isolates from labeo rohita and two from catla catla were ... | 2015 | 25634754 |
| identification and immunogenic potential of b cell epitopes of outer membrane protein ompf of aeromonas hydrophila in translational fusion with a carrier protein. | aeromonas hydrophila, a ubiquitous and virulent bacterial pathogen, affects a variety of fishes, including labeo rohita. existing treatment strategies comprise antibiotic therapies and attenuated bacterial strain-based vaccines. no functional subunit vaccine has been available until now. given their key role in determining pathogenicity, outer membrane proteins have been successfully explored as potential vaccine candidates. we have devised a direct strategy for eliminating non-specific response ... | 2015 | 25636835 |
| immunomodulatory effect of arsenic on cytokine and hsp gene expression in labeo rohita fingerlings. | immune system is fundamental for survival of an organism against invading pathogens and other harmful agents. cytokines, the signaling proteins that are produced transiently after cell activation and exert pleiotropic effects on cells of the immune system, are important mediators of cell mediated immune response. when expressed in a dysregulated fashion cytokines can underlie either immunodeficient or immunopathologic states. heat shock proteins (stress proteins, hsps) are also key proteins, whi ... | 2015 | 25652292 |
| parenteral immunization of pla/plga nanoparticle encapsulating outer membrane protein (omp) from aeromonas hydrophila: evaluation of immunostimulatory action in labeo rohita (rohu). | advanced vaccine research approaches needs to explore on biodegradable nanoparticles (nps) based vaccine carrier that can serve as antigen delivery systems as well as immuno-stimulatory action to induce both innate and adaptive immune response in fish. immunogenicity of pla and plga nps encapsulating outer membrane protein (omp) antigen of aeromonas hydrophila were evaluated through intra-peritoneal injection in fish, labeo rohita. antigen loaded pla-omp (223.5 ± 13.19 nm) and plga-omp (166.4 ± ... | 2015 | 25689492 |
| sodium cyanide induced alteration in the whole animal oxygen consumption and behavioural pattern of freshwater fish labeo rohita. | sodium cyanide is a common environmental pollutant which is mainly used in many industries such as mining, electroplating, steel manufacturing, pharmaceutical production and other specialized applications including dyes and agricultural products. it enters aquatic environment through effluents from these industries. static renewal bioassay test has been conducted to determine lc, of sodium cyanide on indigenous freshwater carp, labeo rohita. the behavioural pattern and oxygen consumption were ob ... | 2015 | 25895263 |
| isolation and characterization of flavobacterium columnare from freshwater ornamental goldfish carassius auratus. | filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. the causative agent was identified as flavobacterium columnrae, on the basis of biochemical test, species-specific polymerase chain reaction (pcr) and sequencing of 16s rdna gene with the universal bacterial primers. furthermore, the strain (ing-1) attributed to genomovar ii in 16s rdna pcr-restriction fragment length polymorphism (pcr ... | 2015 | 25895267 |
| dietary inclusion of protease producing novel pontibacter spp. and bacillus megaterium as a probiotic enhances immune responses in labeo rohita. | abstract: this study stresses the key role which can be played by tannery fleshing (tf) hydrolyzing probiotic pontibacter spp. in aqua feed formulation and identifies the probiotic strains in the fish gut capable of enhancing the overall growth and immune responses. probiotics included are pontibacter species (pb) and bacillus megaterium (bm) wherein lactobacillus (lb) served as control. experimental diets includes tannery fleshing (tf1), tf+lb strain (tf2), tf+bm strain (tf3), tf+pb strain (tf4 ... | 2014 | 25911831 |
| first evidence of molecular characterization of rohu carp sox2 gene being expressed in proliferating spermatogonial cells. | because little is known about the function of sox2 (sry-related box-2) in teleosts, the objective of this study was to clone and characterize sox2 complementary dna (cdna) from the testis of indian major carp, labeo rohita (rohu). the full-length cdna contained an open reading frame of 936 nucleotides bearing the typical structural features. phylogenetically, sox2 of l rohita was most closely related to freshwater counterparts than marine water. the sequence information of cdna and genomic dna t ... | 2015 | 25913275 |
| isolation of potential probiotic bacillus spp. and assessment of their subcellular components to induce immune responses in labeo rohita against aeromonas hydrophila. | bacillus species isolated from the gut of healthy labeo rohita (hamilton) were screened for antibacterial activity against selected fish pathogens. among the isolates, kadr5 and kadr6 showed antibacterial activity, tolerated low ph and high bile concentrations and were susceptibility to various antibiotics. based on morphological and biochemical tests and 16s rrna gene analysis the probiotic strains kadr5 and kadr6 were identified as bacillus licheniformis and bacillus pumilus, respectively. the ... | 2015 | 25917974 |
| ontogeny, tissue distribution and expression analysis of igz in rohu, labeo rohita in response to various stimuli. | this study reports presence of igz transcripts, its ontogeny, tissue distribution and expression following various stimulations/infections in rohu. the derived rohu igz sequence clustered together with igz of ctenopharyngodon idella and cyprinus carpio in phylogenetic analysis. igz expression was detected at early developmental stages with higher expression at 1 day post-fertilization, and higher in anterior kidney and lower in skin tissues of juveniles. an inductive expression of igz was observ ... | 2015 | 26055084 |
| effect of guava leaves on the growth performance and cytokine gene expression of labeo rohita and its susceptibility to aeromonas hydrophila infection. | the present study aimed to evaluate the effects of psidium guajava l. (guava) leaves on the growth and immune response of the fish species labeo rohita and its susceptibility to aeromonas hydrophila infection. diets containing five different concentrations of guava leaves (0% [basal diet], 0.1% [g1], 0.5% [g2], 1% [g3], and 1.5% [g4]) were fed to fish (average weight: 11.1 g) for 60 days. various growth and immune parameters were examined 60 days post-feeding. fish were challenged with a. hydrop ... | 2015 | 26093209 |
| neuroanatomical localization of nitric oxide synthase (nnos) in the central nervous system of carp, labeo rohita during post-embryonic development. | nitric oxide (no) is a chemically diffusible molecular messenger playing various roles in both vertebrates and invertebrates. nitric oxide synthase (nos) is the key enzyme in synthesis of no. the neuroanatomical distribution pattern of neuronal nitric oxide synthase (nnos) was studied and developing stages of labeo rohita such as hatchlings (10-15mm), frys (15-35mm), semi-fingerlings (35-65mm), fingerlings (65-100mm) and adults (350-370mm) were used. in the telencephalon, nitrergic cells were ob ... | 2015 | 26143285 |
| construction, de-novo assembly and analysis of transcriptome for identification of reproduction-related genes and pathways from rohu, labeo rohita (hamilton). | rohu is a leading candidate species for freshwater aquaculture in south-east asia. unlike common carp the monsoon breeding habit of rohu restricts its seed production beyond season indicating strong genetic control over spawning. genetic information is limited in this regard. the problem is exacerbated by the lack of genomic-resources. we identified 182 reproduction-related genes previously by sanger-sequencing which were less to address the issue of seasonal spawning behaviour of this important ... | 2015 | 26148098 |
| preliminary genetic linkage map of indian major carp, labeo rohita (hamilton 1822) based on microsatellite markers. | linkage map with wide marker coverage is an essential resource for genetic improvement study for any species. sex-averaged genetic linkage map of labeo rohita, popularly known as 'rohu', widely cultured in the indian subcontinent, was developed by placing 68 microsatellite markers generated by a simplified method. the parents and their f1 progeny (92 individuals) were used as segregating populations. the genetic linkage map spans a sex-averaged total length of 1462.2 cm, in 25 linkage groups. th ... | 2015 | 26174674 |
| the β-actin gene promoter of rohu carp (labeo rohita) drives reporter gene expressions in transgenic rohu and various cell lines, including spermatogonial stem cells. | we previously characterized the β-actin gene promoter of indian domesticated rohu carp (labeo rohita) and made a reporter construct via fusion to green fluorescence protein (gfp) cdna. in this study, the same construct was used to breed transgenic rohu fish. about 20% of the transgenic offspring showed ubiquitous expression of the reporter gfp gene. in a few of the transgenic fish, we documented massive epithelial and/or muscular expression with visible green color under normal light. the expres ... | 2015 | 26204405 |
| effect of cellular products of potential probiotic bacteria on the immune response of labeo rohita and susceptibility to aeromonas hydrophila infection. | in the present study, the immunological efficacy of cellular components from the potential probiotic bacteria bacillus subtilis vsg1, pseudomonas aeruginosa vsg2, and lactobacillus plantarum vsg3 was evaluated in labeo rohita fingerlings. fish were immunized intraperitoneally with 0.1 ml phosphate-buffer solution (pbs) containing 0.1 mg of any of the following cellular components: intercellular products (icps) of vsg1 (bs-icps), heat-killed whole cell products of vsg2 (pa-hkwcps), or icps of vsg ... | 2015 | 26282681 |
| myxobolus nanokiensis sp. nov. (myxozoa: bivalvulidae), a new pathogenic myxosporean parasite causing haemorrhagic gill disease in cultured indian major carp fish, labeo rohita (hamilton 1822) in punjab, india. | the plasmodia of myxobolus nanokiensis sp. nov. were found infecting gills of labeo rohita (hamilton 1822) the infection rate was found to be 36.67 % (30 fishes were examined and 11 fishes were infected) in the nanoki pond in patiala district punjab. numerous minute plasmodia each filled with 150-200 spores were detected. smear of scrapped blood-tinged mucous from gills exhibited millions of spores. histological sections also indicated numerous plasmodia measuring 38.33-40.33 μm in diameter in t ... | 2015 | 26345042 |
| mitigation of immunosuppressive and oxidative stress effect of dietary gelatinized starch in labeo rohita fingerlings by elevation of rearing temperature within optimum range. | the present study was conducted to investigate the strategy to mitigate the immunosuppressive and oxidative stress effect of gelatinized starch in fingerling of labeo rohita. fingerlings were either maintained at ambient water temperature (26 °c) or exposed to 32 °c for one week and then subjected to 26 °c for four weeks. both groups were fed with isoproteinous (30% crude protein) diets containing gelatinized (g) or non-gelatinized (ng) starch. after 5 weeks of feeding trial, fingerlings were ch ... | 2015 | 26475365 |
| four pro-inflammatory cytokines of rohu (labeo rohita) during early developmental stages, their tissue distribution and expression by leucocytes upon in-vitro stimulation. | cytokines are important components of both adaptive and innate immunity, and are required to initiate and regulate immune responses following infection. the ontogeny and tissue specific distribution of four pro-inflammatory cytokines, interleukin-6 (il-6), tumor necrosis factor α (tnf-α), il-8 and il-1β in rohu (labeo rohita), and their responses by leucocytes from anterior-kidney/head-kidney (hkls), spleen (spls) and peripheral blood (pbls) following stimulation with concanavalin a (cona), cona ... | 2015 | 26518505 |
| effects of intracellular products of bacillus subtilis vsg1 and lactobacillus plantarum vsg3 on cytokine responses in the head kidney macrophages of labeo rohita. | the efficiency of intracellular products (icps) of the probiotics bacillus subtilis vsg1 and lactobacillus plantarum vsg3 in stimulating cytokine responses in the head kidney (hk) macrophages of labeo rohita was investigated. the hk macrophages were incubated with icps and lipopolysaccharide (lps), and the responses of cytokine genes, namely interleukin-10 (il-10), il-1β, il-12p35, il-12p40, il-18, tumour necrosis factor-α (tnf-α), nuclear factor kappa b (nf-κb), cyclo-oxygenase-2 (cox-2), inter ... | 2015 | 26520566 |
| chlorophytum borivilianum polysaccharide fraction provokes the immune function and disease resistance of labeo rohita against aeromonas hydrophila. | the present study aimed to investigate the effects of chlorophytum borivilianum polysaccharide (cbp), as a dietary supplement administered at varying concentrations with feed (basal diet), on various cytokine-related responses in labeo rohita fingerlings. immune parameters and immune-related gene expressions were measured at 3rd, 4th, and 5th week after feeding. the results revealed that dietary administration of cbp at 0.2% and 0.4% for 4 weeks significantly upregulated serum lysozyme and phago ... | 2015 | 26649325 |
| new host record of five flavobacterium species associated with tropical fresh water farmed fishes from north india. | yellow pigmented, filamentous, gram-negative bacteria belonging to genus flavobacterium are commonly associated with infections in stressed fish. in this study, inter-species diversity of flavobacterium was studied in apparently healthy freshwater farmed fishes. for this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. labeo rohita, catla catla and cyprinus carpio. among them, only twelve bacterial isolates (13.18%) were id ... | 2017 | 26691454 |
| recovery of schistosoma haematobium ovum from labeo rohita: first report from pakistan. | schistosomiasis, also known as bilharzia, is a parasitic disease caused by trematodes from the genus schistosoma that can infect humans and animals. s. mansoni, s. japonicum, and s. mekongi all causes intestinal schistosomiasis except s. haematobium that causes urinary schistosomiasis. it is only specie which effects urinary system, it can affect liver, heart, lungs also but very rarely. schistosoma haematobium is endemic to over 50 countries in africa and the middle east and western asia and ma ... | 2015 | 26695198 |
| population genetic characterization and family reconstruction in brood bank collections of the indian major carp labeo rohita (cyprinidae:cypriniformes). | the founder stock of a captive breeding program is prone to changes in genetic structure due to inbreeding and genetic drift. genetic characterization of the founder population using suitable molecular markers may help monitor periodic changes in the genetic structure in future. to develop benchmark information about the genetic structure we analyzed six microsatellite loci in the brodbank collections of rohu (labeo rohita) originated from three major rivers-the jamuna, the padma and the halda. ... | 2015 | 26697284 |
| antagonistic activity of cellular components of potential probiotic bacteria, isolated from the gut of labeo rohita, against aeromonas hydrophila. | the objective of this study was to characterise the antagonistic activity of cellular components of potential probiotic bacteria isolated from the gut of healthy rohu (labeo rohita), a tropical freshwater fish, against the fish pathogen, aeromonas hydrophila. three potential probiotic strains (referred to as r1, r2, and r5) were screened using a well diffusion, and their antagonistic activity against a. hydrophila was determined. biochemical tests and 16s rrna gene analysis confirmed that r1, r2 ... | 2011 | 26781682 |
| characteristics of bacterial isolates from the gut of freshwater fish, labeo rohita that may be useful as potential probiotic bacteria. | in this study, we aimed to evaluate the in vitro probiotic characteristics of three bacteria, lactobacillus plantarum vsg3, pseudomonas aeruginosa vsg2, and bacillus subtilis vsg1, isolated from the gut of labeo rohita. the bacterial isolates tolerated low ph and high bile concentrations in the fish well. the bacterial adhesion capacity to fish intestinal mucosa revealed that the three potential probiotic isolates had a significantly higher adhesion capacity compared to the pathogenic strains te ... | 2012 | 26782183 |
| heat-killed whole-cell products of the probiotic pseudomonas aeruginosa vsg2 strain affect in vitro cytokine expression in head kidney macrophages of labeo rohita. | present study was undertaken to investigate the efficiency of heat-killed whole-cell products (hkwcps) of probiotic pseudomonas aeruginosa vsg2 strain in stimulating the cytokine responses in the head kidney (hk) macrophages of labeo rohita. the hk macrophages were incubated with hkwcps or lipopolysaccharide (lps), and the responses of cytokine genes, namely interleukin-10 (il-10), il-1β, il-p35, il-12p40, tumour necrosis factor-α (tnf-α), nuclear factor kappa b (nf-κb), cyclooxygenase-2 (cox-2) ... | 2016 | 26876356 |
| prevalence, site and tissue preference of myxozoan parasites infecting gills of cultured fish in punjab (india). | native carp species cultured in indian farms in punjab (catla catla catla, rohu labeo rohita, mrigal cirrhinus mrigala, exotic carps such as silver carp hypophthalmichthys molitrix, grass carp ctenopharyngodon idella, common carp cyprinus carpio and a catfish sperata seenghala) were examined for the presence of myxozoan parasites infecting gills. firstly, the gills were examined under a zoom-stereomicroscope for the presence of plasmodia. the number of plasmodia per gill was counted to determine ... | 2016 | 26912043 |
| identification of deleterious mutations in myostatin gene of rohu carp (labeo rohita) using modeling and molecular dynamic simulation approaches. | the myostatin (mstn) is a known negative growth regulator of skeletal muscle. the mutated myostatin showed a double-muscular phenotype having a positive significance for the farmed animals. consequently, adequate information is not available in the teleosts, including farmed rohu carp, labeo rohita. in the absence of experimental evidence, computational algorithms were utilized in predicting the impact of point mutation of rohu myostatin, especially its structural and functional relationships. t ... | 2016 | 27019850 |
| role of bacillus subtilis vsg4-derived biosurfactant in mediating immune responses in labeo rohita. | this study aimed to isolate biosurfactant from co2-sequestering bacillus subtilis vsg4 and to evaluate its immunostimulatory effect in labeo rohita fingerlings. fish were injected intraperitoneally (i.p.) with 0.1 ml of phosphate-buffered saline (pbs) containing the water-soluble fraction of purified biosurfactant at 50 (s50), 100 (s100), 200 (s200), or 300 (s300) μg ml(-1). fish injected with pbs served as controls. various immunological parameters, including immune-related gene expression, wer ... | 2016 | 27079425 |
| establishing targeted carp tlr22 gene disruption via homologous recombination using crispr/cas9. | recent advances in gene editing techniques have not been exploited in farmed fishes. we established a gene targeting technique, using the crispr/cas9 system in labeo rohita, a farmed carp (known as rohu). we demonstrated that donor dna was integrated via homologous recombination (hr) at the site of targeted double-stranded nicks created by crispr/cas9 nuclease. this resulted in the successful disruption of rohu toll-like receptor 22 (tlr22) gene, involved in innate immunity and exclusively prese ... | 2016 | 27079451 |
| variation in susceptibility pattern of fish to argulus siamensis: do immune responses of host play a role? | branchiuran ectoparasites of the genus argulus can have extensive damaging effects on cultured fish. there exist no systematic studies that evaluate susceptibility or resistance of various carp species to argulus sp. and the underlying mechanisms. the present study aimed at identifying the most susceptible and resistant cultured species, studying settlement and survival of parasite on these species, and finally unravelling the variations of immune response in both resistant and susceptible speci ... | 2016 | 27084477 |
| first report on fish cysteine as a biomarker of contamination in the river chenab, pakistan. | the eastern and southern parts of the faisalabad city produce considerable quantities of industrial and municipal pollutants, much of which is drained into the river chenab, reducing the productivity of fauna and flora in the river. this study was aimed to determine whether cysteine is useful as a biomarker of exposure to polluted fresh water. the amino acid profile of fish muscle was analyzed by paper chromatography in cirrhinus mrigala and labeo rohita from the river chenab to determine habita ... | 2016 | 27117257 |
| detection of goldfish haematopoietic necrosis herpes virus (cyprinid herpesvirus-2) with multi-drug resistant aeromonas hydrophila infection in goldfish: first evidence of any viral disease outbreak in ornamental freshwater aquaculture farms in india. | this outbreak report details of a mortality event where cyprinid herpes virus-2 (cyhv-2) was detected in association with multidrug-resistant aeromonas hydrophila infection in goldfish, carassius auratus, from commercial farms. the goldfish exhibited large scale haemorrhages on the body, fins and gills, lepidorthosis, necrosed gills, protruded anus and shrunken eyes. white nodular necrotic foci in spleen and kidneys were noticed, along with necrosis and fusion of gill lamellae. transmission elec ... | 2016 | 27172876 |
| aeromonas hydrophila ompw plga nanoparticle oral vaccine shows a dose-dependent protective immunity in rohu (labeo rohita). | aeromonas hydrophila is a gram-negative bacterium that causes high mortality in different fish species and at different growth stages. although vaccination has significantly contributed to the decline of disease outbreaks in aquaculture, the use of oral vaccines has lagged behind the injectable vaccines due to lack of proven efficacy, that being from primary immunization or by use of boost protocols. in this study, the outer membrane protein w (ompw) of a. hydrophila was cloned, purified, and en ... | 2016 | 27258315 |
| dietary administration of banana (musa acuminata) peel flour affects the growth, antioxidant status, cytokine responses, and disease susceptibility of rohu, labeo rohita. | to explore the feasibility of musa acuminata (banana) peels as a feed additive, effects of banana peel flour (bpf) on the growth and immune functions of labeo rohita were evaluated. diets containing five different concentrations of bpf (0% [basal diet], 1% [b1], 3% [b3], 5% [b5], and 7% [b7]) were fed to the fish (average weight: 15.3 g) for 60 days. the final weight gain and specific growth rate were higher (p < 0.05) in the b5 group. the most significant improvements in immune parameters such ... | 2016 | 27294156 |
| molecular identification of a new myxozoan, myxobolus dermiscalis n. sp. (myxosporea) infecting scales of labeo rohita hamilton in harike wetland, punjab (india). | in the present study, a new species myxobolus dermiscalis n. sp. infecting scales of labeo rohita, an indian major carp from harike wetland in punjab, india has been described on the basis of spore morphology and amplification of a part of 18s rdna gene. the pseudocysts of m. dermiscalis n. sp. are milky white with irregular outline, 0.5-3.6 mm in diameter embedded within the dermal scale in the form of a cavity. the spores 5.84-7.98 × 3.98-5.98 μm in size, having two equal polar capsules 3.98-5 ... | 2016 | 27330981 |
| apolipoprotein a-i in labeo rohita: cloning and functional characterisation reveal its broad spectrum antimicrobial property, and indicate significant role during ectoparasitic infection. | apolipoprotein a-i (apoa-i) is the most abundant and multifunctional high-density lipoprotein (hdl) having a major role in lipid transport and potent antimicrobial activity against a wide range of microbes. in this study, a complete cds of 771 bp of labeo rohita (rohu) apoa-i (lrapoa-i) encoding a protein of 256 amino acids was amplified, cloned and sequenced. tissue specific transcription analysis of lrapoa-i revealed its expression in a wide range of tissues, with a very high level of expressi ... | 2016 | 27368542 |
| evaluation of pollution of ganga river water using fish as bioindicator. | ganga river, life line of millions of people got heavily polluted due to uncontrolled anthropogenic activities. to monitor the effect of pollution of the river on its aquatic life, a field study was conducted by analyzing the different biomarker enzymes and biochemical parameters in the various tissues (muscles, liver, gills, kidney, brain, and skin) of the indian major carp labeo rohita collected from the river ganga from different study sites of varanasi district. activities of antioxidant enz ... | 2016 | 27370309 |
| enzymatic extraction and characterisation of a thermostable collagen from swim bladder of rohu (labeo rohita). | the fish swim bladder is considered as a potential source of realistic collagen. currently, processing of the indian major carp rohu (labeo rohita) generates an enormous quantity of non-edible by-products, including swim bladders, which are discarded as waste with no commercial value. in the present study, collagen was prepared from rohu swim bladder and its physicochemical and fibril-forming capacities were assessed. | 2017 | 27380938 |
| b cell activating factor is induced by toll-like receptor and nod-like receptor-ligands and plays critical role in igm synthesis in labeo rohita. | b-cell activating factor (baff), an important member of the tumor necrosis factor superfamily, plays critical roles in the modulation of b-cell functions and enhancement of immune response in the host. like higher vertebrates, the important role of baff in boosting immune response against diverse pathogens was also envisaged in fishes. we therefore, studied baff in rohu (labeo rohita), a freshwater food fish species of highest economic importance in the indian subcontinent. full-length rohu-baff ... | 2016 | 27568001 |
| role of dietary ginger zingiber officinale in improving growth performances and immune functions of labeo rohita fingerlings. | this study evaluated the effects of ginger (zingiber officinale) as a feeding supplement on the growth, skin mucus immune parameters, and cytokine-related gene expression of labeo rohita, and its susceptibility to aeromonas hydrophila infection. diets containing six different concentrations of dried ginger (0% [basal diet], 0.2% [g2], 0.4% [g4], 0.6% [g6], 0.8% [g8], and 1.0% [g10] were fed to fish (average weight: 12.3 g) for 60 days. growth parameters were examined at 30 and 60 days post-feedi ... | 2016 | 27574828 |
| molecular characterization of activin receptor type iia and its expression during gonadal maturation and growth stages in rohu carp. | activin receptor type iia (actriia), a transmembrane serine/threonine kinase receptor is an important regulator of physiological traits, viz., reproduction and body growth in vertebrates including teleosts. however, existing knowledge of its role in regulating fish physiology is limited. to address this, we have cloned and characterized the actriia cdna of labeo rohita (rohu), an economically important fish species of the indian subcontinent. comparative expression profiling of the receptor gene ... | 2017 | 27575753 |
| assessment of hemolytic activity, enzyme production and bacteriocin characterization of bacillus subtilis lr1 isolated from the gastrointestinal tract of fish. | in the present investigation, probiotic potential (antagonistic activity, enzyme production, hemolytic activity, biosafety, antibiotic sensitivity and bile tolerance level) of bacillus subtilis lr1 was evaluated. bacteriocin produced by the bacterial strain b. subtilis lr1 isolated from the gastrointestinal tract of labeo rohita was purified and characterized. the molecular weight of the purified bacteriocin was ~50 kda in 12 % native page and showed inhibitory activity against four fish pathoge ... | 2017 | 27590016 |
| immunoglobulin (ig) d in labeo rohita is widely expressed and differentially modulated in viral, bacterial and parasitic antigenic challenges. | immunoglobulins (igs) play critical roles in protecting host against diverse pathogenic invasion and diseases. among all ig isotypes, igd is the most recently-evolved and enigmatic molecule detected in all vertebrates species except birds. in south-east asia, labeo rohita (rohu) is the leading candidate fish species for freshwater aquaculture, and this article describes about igd gene expression in rohu following viral, bacterial and parasitic antigenic challenges. the partial cdna (761bp) of la ... | 2016 | 27590429 |
| structural and functional characterization of recombinant interleukin-10 from indian major carp labeo rohita. | interleukin-10, an important regulator of both the innate and adaptive immune systems, is a multifunctional major cytokine. though it is one of the major cytokines, il-10 from the indian major carp, labeo rohita, has not yet been characterized. in the present study, we report large scale production and purification of biologically active recombinant il-10 of l. rohita (rlril-10) using a heterologous expression system and its biophysical and functional characterization. high yield (~70 mg/l) of s ... | 2017 | 27689097 |
| comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen. | collagen and collagen fibers have been widely documented as a potential and competitive biomaterial for medical applications. however, the searches for safe and realistic new collagen sources are still underway. currently, fishery by-products (scales), a promising collagen source are usually discarded. in the present study, in vitro fibril-forming ability of the extracted fish scale collagen is reported. the aim of the investigation was to evaluate the concomitant comparison of fibril-forming ab ... | 2017 | 27770898 |
| dietary protein enhances non-specific immunity, anti-oxidative capability and resistance to aeromonas hydrophila in labeo rohita fingerlings pre-exposed to short feed deprivation stress. | present experiment was conducted to study the effect of dietary protein levels on growth, immunity and anti-oxidative status of labeo rohita fingerlings during feed deprivation followed by refeeding. fish (5.44 ± 0.10 g) were deprived of feed for 3 weeks and then re-fed to satiation for 5 weeks with one of the diets containing 25 (25p), 30 (30p), 35 (35p) or 40 (40p) percent crude protein (cp) level. in addition to these groups, a control group (c) was also maintained by feeding to satiation lev ... | 2016 | 27815199 |
| fish scales as a non-lethal tool of the toxicity of wastewater from the river chenab. | water pollution is gradually increasing in natural waters through anthropogenic activities. this study aimed to use fish scales as a bio-indicator of pollution, along with water quality parameters, and the assessment and detection of selected heavy metals in water samples collected from the river chenab, including the chakbandi drain that gathers domestic sewage waste and industrial effluents from faisalabad and deposits it into this freshwater body. all water quality parameters (ph, total disso ... | 2017 | 27817145 |
| molecular cloning and characterization of lrtlr4, analysis of its inductive expression and associated down-stream signaling molecules following lipopolysaccharide stimulation and gram-negative bacterial infection. | toll-like receptors (tlrs) play key roles in innate immunity from lower to higher vertebrates. among various tlr types, tlr4 was reported to recognize lps in higher vertebrates resulting in the activation of down-stream signaling pathway. except in some teleosts, function of tlr4 in most fish species including rohu (labeo rohita) a commercially important fish species in the south-east asian countries remained unknown. to investigate it, full-length cdna of labeo rohita tlr4 (lrtlr4) was cloned, ... | 2017 | 27838566 |
| description of a myxozoan parasite myxobolus sp.n pkb2014 from an edible fish, with emphasis on its molecular characterization. | in india, more than 104 species of myxobolus have so far been reported infecting freshwater as well as marine fishes. the study focuses on the description of a new myxosporean species, myxobolus sp.n pkb 2014 from the gill lamellae of an indian major carp labeo rohita. the species have been described on the basis of morphological characterization of the spores, tissue architecture and 18s rdna sequence data. the plasmodia of myxobolus sp.n pkb 2014 were round in shape measuring, 50 to 70 μm in d ... | 2015 | 27844012 |
| population structure and genetic diversity of indian major carp, labeo rohita (hamilton, 1822) from three phylo-geographically isolated riverine ecosystems of india as revealed by mtdna cytochrome b region sequences. | the population structure and genetic diversity of rohu (labeo rohita hamilton, 1822) was studied by analysis of the partial sequences of mitochondrial dna cytochrome b region. we examined 133 samples collected from six locations in three geographically isolated rivers of india. analysis of 11 haplotypes showed low haplotype diversity (0.00150), nucleotide diversity (π) (0.02884) and low heterogeneity value (0.00374). analysis of molecular variance (amova) revealed the genetic diversity of l. roh ... | 2016 | 28024428 |
| first record of myxozoan parasites from fresh water fishes of jammu and kashmir and their pathogenecity. | the present study was aimed to isolate myxozoan parasites infecting the native carp schizothorax esocinus (heckel) and pond reared carp labeo rohita (ham.) collected from fresh water ecosystems of jammu and kashmir from july 2013 to june 2014. various organs such as eyes, gills, scales, fins, intestines, visceral organs and pericardial cavity of as many as 100 live specimens were examined. results revealed that 14% of the examined l. rohita were infected with myxobolus rocatlae (ham.) and 6% of ... | 2017 | 28232055 |
| myxobolus chushi n. sp. (myxozoa:myxosporea) parasitizing schizothorax niger (heckel), a native cyprinid fish from wullar lake in kashmir himalayas. | in the study, a new species, myxobolus chushi n. sp. infecting gills of wild specimens of schizothorax niger (heckel) inhabiting wullar lake in kashmir himalayas, (j&k) india has been described based on morphology of the myxospore and using partial 18s rdna sequencing. pathological changes in the gills have been studied with the help of histological sections stained with luna's method. twenty fish specimens were examined, out of which four had oval, white plasmodia in gills measuring 2.0×0.5mm. ... | 2017 | 28232063 |
| morphotaxonomical description of three new species of myxobolus bütschli, 1882 (myxozoa: myxosporea: bivalvulida) from edible freshwater fishes of west bengal, india. | three new species of myxobolus are described from the mucous membrane around gill lamellae of carps of west bengal. the new species have been named as m. muralidharani sp. n., m. nilimae sp. n. from labeo rohita, and m. harpreetae sp. n. from labeo bata respectively. spores of m. muralidharani sp. n. measured 13.84 ± 0.049 (13-14) × 4.95-6 (5.35) µm, with two elongated equal sized polar capsules. spores of m. nilimae sp. n. measured 15.9-17.1 (16.65) µm × 6.2-7.5 (6.9) µm, with two equal petal s ... | 2017 | 28316395 |
| description of three new species of the genus myxobolus infecting carp fishes of india. | myxobolus bütschli, 1882 (myxozoa, myxosporea, bivalvulida, myxobolidae) is an important parasitic protozoan of freshwater fishes reported from almost all over the world. the severity of infection may lead to mortality of the host fish. the present paper deals with the description of three new species of myxobolus bütschli, 1882, myxobolus sonarpurensis sp. nov., myxobolus elongatum sp. nov. and myxobolus petalum sp. nov. from a freshwater minor carp labeo bata (hamilton, 1822) and major carp la ... | 2017 | 28316405 |
| bioaccumulation of some heavy metals: analysis and comparison of cyprinus carpio and labeo rohita from sardaryab, khyber pakhtunkhwa. | we examined and compared heavy metals bioaccumulation in cyprinus carpio and labeo rohita netted from sardaryab, a tributary of river kabul. by using atomic absorption spectrometry we assessed different organs including livers, gills, and muscles. metals studied were chromium, iron, zinc, lead, and copper. livers of both species showed higher concentrations of metals while muscles showed the least amount. chromium and iron were the highly concentrated metals in the gills and livers of both speci ... | 2017 | 28396869 |
| role of bacillus licheniformis vs16-derived biosurfactant in mediating immune responses in carp rohu and its application to the food industry. | multifarious applications of bacillus licheniformis vs16-derived biosurfactant were explored. labeo rohita fingerlings were injected intraperitoneally with 0.1 ml of phosphate-buffered saline (pbs) containing purified biosurfactant at 0 (control), 55 (s55), 110 (s110), 220 (s220), or 330 (s330) μg ml(-1) concentrations. various immunological parameters and the expression of immune-related genes were measured at 7, 14, and 21 days post-administration (dpa). at 21 dpa, fish were challenged with ae ... | 2017 | 28400765 |