| molecular cloning and characterization of nucleotide binding and oligomerization domain-1 (nod1) receptor in the indian major carp, rohu (labeo rohita), and analysis of its inductive expression and down-stream signalling molecules following ligands exposure and gram-negative bacterial infections. | nucleotide binding and oligomerization domain-1 (nod1) is a cytoplasmic pattern recognition receptor (prr), and is a member of the nod-like receptor (nlr) family. it senses a wide range of bacteria and viruses or their products, and plays a key role in inducing innate immunity. in this report, nod1 gene was cloned and characterized in rohu (labeo rohita), a fish species of highest commercial importance in the indian subcontinent. the full-length rohu nod1 (rnod1) cdna comprised of 3168 bp with a ... | 2012 | 22530240 |
| expressed sequences and polymorphisms in rohu carp (labeo rohita, hamilton) revealed by mrna-seq. | expressed genes and polymorphisms were identified in lines of rohu labeo rohita selected for resistance or susceptibility to aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis. all animals were grown in a common environment and rna from ten individuals from each line pooled for illumina mrna-seq. de novo transcriptome assembly produced 137,629 contigs with 40× average coverage.forty-four percent of the assembled sequences were annotated with gene names and ontology terms. ... | 2012 | 22298294 |
| antagonistic activity of cellular components of potential probiotic bacteria, isolated from the gut of labeo rohita, against aeromonas hydrophila. | the objective of this study was to characterise the antagonistic activity of cellular components of potential probiotic bacteria isolated from the gut of healthy rohu (labeo rohita), a tropical freshwater fish, against the fish pathogen, aeromonas hydrophila. three potential probiotic strains (referred to as r1, r2, and r5) were screened using a well diffusion, and their antagonistic activity against a. hydrophila was determined. biochemical tests and 16s rrna gene analysis confirmed that r1, r2 ... | 2011 | 26781682 |
| characterization of bacillus spp. from the gastrointestinal tract of labeo rohita--towards to identify novel probiotics against fish pathogens. | the aim of the present study is to screen and characterize endogenous microbiota bacillus spp. from the gastrointestinal (gi) tract of labeo rohita in order to evaluate their probiotic attributes. a total of 74 isolates from the gi of l. rohita were evaluated for their antimicrobial properties by agar well-diffusion method against fish pathogens. based on the better antibacterial features, three isolates (kadr1, kadr3, and kadr4) were selected for further delineation. the three selected isolates ... | 2015 | 25274116 |
| effects of intracellular products of bacillus subtilis vsg1 and lactobacillus plantarum vsg3 on cytokine responses in the head kidney macrophages of labeo rohita. | the efficiency of intracellular products (icps) of the probiotics bacillus subtilis vsg1 and lactobacillus plantarum vsg3 in stimulating cytokine responses in the head kidney (hk) macrophages of labeo rohita was investigated. the hk macrophages were incubated with icps and lipopolysaccharide (lps), and the responses of cytokine genes, namely interleukin-10 (il-10), il-1β, il-12p35, il-12p40, il-18, tumour necrosis factor-α (tnf-α), nuclear factor kappa b (nf-κb), cyclo-oxygenase-2 (cox-2), inter ... | 2015 | 26520566 |
| beneficial effects of dietary probiotics mixture on hemato-immunology and cell apoptosis of labeo rohita fingerlings reared at higher water temperatures. | probiotics play an important role in growth increment, immune enhancement and stress mitigation in fish. increasing temperature is a major concern in present aquaculture practices as it markedly deteriorates the health condition and reduces the growth in fish. in order to explore the possibilities of using probiotics as a counter measure for temperature associated problems, a 30 days feeding trial was conducted to study the hemato-immunological and apoptosis response of labeo rohita (8.3±0.4 g) ... | 2014 | 24979660 |
| characteristics of bacterial isolates from the gut of freshwater fish, labeo rohita that may be useful as potential probiotic bacteria. | in this study, we aimed to evaluate the in vitro probiotic characteristics of three bacteria, lactobacillus plantarum vsg3, pseudomonas aeruginosa vsg2, and bacillus subtilis vsg1, isolated from the gut of labeo rohita. the bacterial isolates tolerated low ph and high bile concentrations in the fish well. the bacterial adhesion capacity to fish intestinal mucosa revealed that the three potential probiotic isolates had a significantly higher adhesion capacity compared to the pathogenic strains te ... | 2012 | 26782183 |
| use of black carp (mylopharyngodon piceus) in biological control of intermediate host snails of fish-borne zoonotic trematodes in nursery ponds in the red river delta, vietnam. | the risks of fish-borne zoonotic trematodes (fzt) to human health constitute an important problem in vietnam. the infection of humans with these trematodes, such as small liver trematodes (clonorchis sinensis and opisthorchis viverrini), intestinal trematodes (heterophyidae) and others is often thought to be linked to fish culture in areas where the habit of eating raw fish is common. juvenile fish produced in nurseries are often heavily infected with fzt and since fishes are sold to aquaculture ... | 2013 | 23680382 |
| outer membrane protein assembly factor yaet (omp85) and groel proteins of edwardsiella tarda are immunogenic antigens for labeo rohita (hamilton). | | 2014 | 24320972 |
| monoclonal antibody to serum immunoglobulins of clarias batrachus and its application in immunoassays. | serum immunoglobulins of clarias batrachus (cb-ig) were purified by affinity chromatography using bovine serum albumin as capture ligand. under reducing conditions in sds-page, cb-ig was composed of a heavy (h) chain (68.7 kda) and two light (l) chains (27.4 and 26.3 kda). purified cb-ig was used to produce a monoclonal antibody (mab) designated e4 mab that belonged to igg1 subclass. in western blotting, this mab showed binding to h chain of purified cb-ig and putative h chains in reduced sera o ... | 2012 | 23000018 |
| role of hsp70 in cytoplasm protection against thermal stress in rohu, labeo rohita. | to understand the function of hsp70 of labeo rohita (lrhsp70) in cellular protection, lrhsp70 orf cdna was inserted into the plasmid of pet-32a(+) or pegfp-l1. then, the recombinant plasmids were transformed or transfected into escherichia coli cells, mouse myeloma cells (mpc-11) or fish hepatoma cells (plhc-1). western blot results revealed that lrhsp70 was expressed in e. coli cells and molecular weight was estimated to be 70 kda. in cells, lrhsp70 was over-expressed following thermal or cold ... | 2014 | 25240978 |
| post-harvest loss of farm raised indian and chinese major carps in the distribution channel from mymensingh to rangpur of bangladesh. | post-harvest loss of catla (catla catla), rohu (labeo rohita), mrigal (cirrhinus mrigala), silver carp (hypophthalmichthys molitrix), grass carp (ctenopharyngodon idella) and sarpunti (puntius sarana) in a single distribution chain from harvest in mymensingh to retail sale rangpur town were determined, in order to obtain information on quality deterioration and existing handling and icing conditions so that suggestions for improving such practices can be made. quality defect points of the fish a ... | 2013 | 24494525 |
| escherichia coli contamination of fish raised in integrated pig-fish aquaculture systems in vietnam. | integrated livestock-fish aquaculture utilizes animal excreta and urine as pond fertilizers to enhance growth of plankton and other microorganisms eaten by the fish. in vietnam, pigs are commonly integrated with fish and horticulture in household-based vac systems (vuon = garden; ao = pond; chuong = pigsty), but the level of fecal contamination in the fish produced is unknown. this study was carried out to assess the level escherichia coli contamination of fish meat and gut contents of grass car ... | 2012 | 22980016 |
| review of the bioenvironmental methods for malaria control with special reference to the use of larvivorous fishes and composite fish culture in central gujarat, india. | mosquito control with the use of insecticides is faced with the challenges of insecticide resistance in disease vectors, community refusal, their high cost, operational difficulties, and environmental concern. in view of this, integrated vector control strategies with the use of larvivorous fishes such as guppy (poecilia reticulata) and gambusia (g. affinis) as biological control agents were used in controlling mosquito breeding in different types of breeding places such as intradomestic contain ... | 2013 | 23703433 |
| isolation and characterization of flavobacterium columnare from freshwater ornamental goldfish carassius auratus. | filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. the causative agent was identified as flavobacterium columnrae, on the basis of biochemical test, species-specific polymerase chain reaction (pcr) and sequencing of 16s rdna gene with the universal bacterial primers. furthermore, the strain (ing-1) attributed to genomovar ii in 16s rdna pcr-restriction fragment length polymorphism (pcr ... | 2015 | 25895267 |
| comparative analysis of innate immune parameters of the skin mucous secretions from certain freshwater teleosts, inhabiting different ecological niches. | the innate immune system of fish is considered first line of defense against a broad spectrum of pathogens. being a component of innate immunity and lying at the interface between fish and the aqueous environment, skin mucus plays a frontier role in protecting fish from infections. in the present study, skin mucus of cirrhinus mrigala, labeo rohita, catla catla, rita rita and channa punctata, inhabiting different ecological niches, was analyzed to characterize potential innate immune factors suc ... | 2012 | 22350522 |
| effects of water borne iron on spawn of indian major carps (catla catla (ham.), labeo rohita (ham.) and cirrhinus mrigala (ham.)). | effects of water-borne iron on indian major carps spawn were evaluated in the present study. ferrous sulphate was used to prepare different test iron concentrations. mrigal had the lowest 96 h lc(50) value of 0.30 ± 0.06 mg l(-1) while rohu had the highest value of 0.73 ± 0.06 mg l(-1) of iron. accumulation of iron in mrigal spawn was highest whereas it was lowest in catla. abnormal behaviour and reduced growth were observed in chronic toxicity. application factors were calculated to establish a ... | 2012 | 23052582 |
| nutrients budget and effluents characteristics in polyculture of scampi (macrobrachium rosenbergii) and indian major carps ponds using organic inputs. | experiments were conducted for the study of nutrient budget in farmers' ponds (0.4-0.6 ha) in orissa, india, at stocking densities of 0.30-0.38/m(2) for scampi (macrobrachium rosenbergii) and 0.60-0.70/m(2) for indian major carps (catla catla, labeo rohita and cirrhinus mrigala). the average initial body weights of scampi and the major carps were 0.02 and 8-10 g, respectively. the culture period was 272-292 days. feed was the main input. the fcr (feed conversion ratio) varied from 1.78 to 1.83. ... | 2012 | 22864442 |
| isolation and characterization of head kidney derived macrophages of labeo rohita. | macrophages play a significant role in non-specific defense mechanisms of all vertebrates against pathogens. one critical element in the area of fish immunology is the unavailability of in-vitro model of immune cells. therefore, it is essential to develop methods for harvesting and culture of macrophages for assessing innate immune functions of rohu, labeo rohita, an important culture fish of india. head kidney leukocytes from were isolated by density gradient sedimentation, so as to exclude oth ... | 2014 | 25204072 |
| study on antimicrobial potential of neem oil nanoemulsion against pseudomonas aeruginosa infection in labeo rohita. | presence of several biochemical constituents in neem makes it an efficient antimicrobial agent for pathogenic diseases. the current investigation was aimed to assess the therapeutic potential of neem nanoemulsion as a control measure for pseudomonas aeruginosa infection in freshwater fish labeo rohita. the median lethal concentration (lc50) for the neem oil and neem nanoemulsion was 73.9 and 160.3 mg/l, respectively. the biomarker enzymes of treated fish tissues showed a significant difference i ... | 2016 | 24502533 |
| fish scales as a non-lethal tool of the toxicity of wastewater from the river chenab. | water pollution is gradually increasing in natural waters through anthropogenic activities. this study aimed to use fish scales as a bio-indicator of pollution, along with water quality parameters, and the assessment and detection of selected heavy metals in water samples collected from the river chenab, including the chakbandi drain that gathers domestic sewage waste and industrial effluents from faisalabad and deposits it into this freshwater body. all water quality parameters (ph, total disso ... | 2017 | 27817145 |
| heat-killed whole-cell products of the probiotic pseudomonas aeruginosa vsg2 strain affect in vitro cytokine expression in head kidney macrophages of labeo rohita. | present study was undertaken to investigate the efficiency of heat-killed whole-cell products (hkwcps) of probiotic pseudomonas aeruginosa vsg2 strain in stimulating the cytokine responses in the head kidney (hk) macrophages of labeo rohita. the hk macrophages were incubated with hkwcps or lipopolysaccharide (lps), and the responses of cytokine genes, namely interleukin-10 (il-10), il-1β, il-p35, il-12p40, tumour necrosis factor-α (tnf-α), nuclear factor kappa b (nf-κb), cyclooxygenase-2 (cox-2) ... | 2016 | 26876356 |
| comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen. | collagen and collagen fibers have been widely documented as a potential and competitive biomaterial for medical applications. however, the searches for safe and realistic new collagen sources are still underway. currently, fishery by-products (scales), a promising collagen source are usually discarded. in the present study, in vitro fibril-forming ability of the extracted fish scale collagen is reported. the aim of the investigation was to evaluate the concomitant comparison of fibril-forming ab ... | 2017 | 27770898 |
| recovery of schistosoma haematobium ovum from labeo rohita: first report from pakistan. | schistosomiasis, also known as bilharzia, is a parasitic disease caused by trematodes from the genus schistosoma that can infect humans and animals. s. mansoni, s. japonicum, and s. mekongi all causes intestinal schistosomiasis except s. haematobium that causes urinary schistosomiasis. it is only specie which effects urinary system, it can affect liver, heart, lungs also but very rarely. schistosoma haematobium is endemic to over 50 countries in africa and the middle east and western asia and ma ... | 2015 | 26695198 |
| prevalence, site and tissue preference of myxozoan parasites infecting gills of cultured fish in punjab (india). | native carp species cultured in indian farms in punjab (catla catla catla, rohu labeo rohita, mrigal cirrhinus mrigala, exotic carps such as silver carp hypophthalmichthys molitrix, grass carp ctenopharyngodon idella, common carp cyprinus carpio and a catfish sperata seenghala) were examined for the presence of myxozoan parasites infecting gills. firstly, the gills were examined under a zoom-stereomicroscope for the presence of plasmodia. the number of plasmodia per gill was counted to determine ... | 2016 | 26912043 |
| new host record of five flavobacterium species associated with tropical fresh water farmed fishes from north india. | yellow pigmented, filamentous, gram-negative bacteria belonging to genus flavobacterium are commonly associated with infections in stressed fish. in this study, inter-species diversity of flavobacterium was studied in apparently healthy freshwater farmed fishes. for this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. labeo rohita, catla catla and cyprinus carpio. among them, only twelve bacterial isolates (13.18%) were id ... | 2017 | 26691454 |
| myxozoan infections of the three indian major carps in fish ponds around meerut, up, india, with descriptions of three new species, myxobolus basuhaldari sp. n., m. kalavatiae sp. n. and m. meerutensis sp. n., and the redescription of m. catlae and m. bhadrensis. | new myxosporean species are described from indian fishes cultured in pond farms of meerut, uttar pradesh (up) state. based upon plasmodia found in the indian major carps (catla catla, cirrhinus cirrhosus, labeo rohita and their hybrids), three new myxobolus spp., myxobolus basuhaldari sp. n., myxobolus kalavatiae sp. n. and myxobolus meerutensis sp. n., are described, and two species, myxobolus catlae and myxobolus bhadrensis, are redescribed. plasmodia of m. basuhaldari sp. n., m. kalavatiae sp ... | 2015 | 25585606 |
| genomic cloning and sequence analysis of interleukin-10 from labeo rohita. | interleukin-10 (il-10) is a pleiotropic cytokine and plays an important role in inflammation, immunoregulation and the pathogenesis of various diseases. therefore, it is our interest to isolate, clone, sequence and characterize il-10 gene from the fish labeo rohita (lr). the gene was amplified using genomic dna isolated from head kidney with primers designed on conserved sequence homologues of fishes belonging to cyprinidae family. the glril-10 is 1467 nucleotides long with five exons and four i ... | 2014 | 25489171 |
| first evidence of comparative responses of toll-like receptor 22 (tlr22) to relatively resistant and susceptible indian farmed carps to argulus siamensis infection. | toll-like receptor 22 (tlr22) is present in teleost but not in mammals. among indian farmed carps, catla catla is relatively more resistant than labeo rohita to argulus siamensis lice infection. tlr22 is believed to be associated with innate immunity against ectoparasite infection. to investigate the tlr22 mediated immunity against argulosis, we have cloned and characterized tlr22 genes of l. rohita (rtlr22) and c. catla (ctlr22). the full-length cdnas of rtlr22 and ctlr22 contained an open read ... | 2014 | 24998226 |
| cross-priming of microsatellite loci in subfamily cyprininae (family cyprinidae): their utility in finding markers for population genetic analysis in three indian major carps. | this study is aimed to identify polymorphic microsatellite markers and establish their potential for population genetics studies in three carp (family cyprinidae; subfamily cyprininae) species, labeo rohita, catla catla and cirrhinus mrigala through use of cyprinid primers. these species have high commercial value and knowledge of genetic variation is important for management of farmed and wild populations. we tested 108 microsatellite primers from 11 species belonging to three different cyprini ... | 2014 | 24792330 |
| effect of ice storage on the functional properties of proteins from a few species of fresh water fish (indian major carps) with special emphasis on gel forming ability. | in the present study the effect of ice storage on physico-chemical and functional properties of proteins from indian major carps with special emphasis on gel forming ability have been assessed for a period of 22 days. the solubility profile of proteins in high ionic strength buffer and calcium adenosine triphosphatase (atpase) enzyme activity reduced significantly (p < 0.05), while that of total volatile base nitrogen (tvb-n) increased significantly (p < 0.05) at the end of 22 days of ice storag ... | 2014 | 24741158 |
| comparative organoleptic quality of indian major carps collected from pond and reservoir. | an attempt was made to distinguish the possible organoleptic differences between the pond reared and wild fish species of reservoirs and correlate these with the physicochemical characteristics of water bodies. the texture was found to firmer in wild varieties of fishes than pond reared fish, with best in catla catla followed by labeo rohita and cirrhinus mrigala. all the cultured fishes had lighter colour than wild fish species which were darker in appearance. comparatively, higher dissolved ox ... | 2013 | 24640254 |
| in vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of catla catla and gill cell line of labeo rohita. | silver nanoparticles (ag-nps) are used in commercial products for their antimicrobial properties. the ag-nps in some of these products are likely to reach the aquatic environment, thereby posing a health concern for humans and aquatic species. the silver nanoparticles were synthesized and characterized using, uv-vis spectra, dynamic light scattering (dls) and transmission electron microscopy (tem) analysis. acute toxicity tests on fish were conducted by exposing catla catla and labeo rohita for ... | 2014 | 24524868 |
| a comparative study of coi and 16 s rrna genes for dna barcoding of cultivable carps in india. | the 5' region of the mitochondrial dna gene cytochrome c oxidase subunit i (coi) is the standard marker for dna barcoding. however, 16 s rrna has also been advocated for dna barcoding in many animal species. herein, we directly compare the usefulness of coi and 16 s rrna in discriminating six cultivable carp species: labeo rohita, catla catla, cirrhinus mrigala, labeo fimbriatus, labeo bata and cirrhinus reba from india. analysis of partial sequences of these two gene fragments from 171 individu ... | 2015 | 24047160 |
| characterization and physical mapping of 18s and 5s ribosomal genes in indian major carps (pisces, cyprinidae). | characterization of the major (18s) and minor (5s) ribosomal rna genes were carried out in three commercially important indian major carp (imc) species, viz. catla catla, labeo rohita and cirrhinus mrigala along with their physical localizations using dual colour fluorescence in situ hybridization. the diploid chromosome number in the above carps was confirmed to be 50 with inter-species karyo-morphological variations. the 18s rdna signals were observed on 3 pair of chromosomes in c. catla and l ... | 2013 | 23587674 |
| peptide mapping of polymorphic myosin heavy chain isoforms in different muscle types of some freshwater teleosts. | a modified sds-page system has been employed to resolve polymorphic myosin heavy chain (myhc) isoforms in different muscle types of three freshwater teleosts displaying different modes of respiration, adaptive features and life styles. investigated species include accessory air-breather channa punctata along with exclusive aquatic breather major carps labeo rohita and catla catla. all the selected species show specificity and expressivity of at least three myhc isoforms, one each in red, head an ... | 2013 | 23086608 |
| impact of exotic carps in the polyculture with indigenous carps: competition for food. | the fingerlings of indigenous carps such as catla (catla catla), rohu (labeo rohita) and mrigal (cirrhinus mrigala) with exotic carps such as silver carp (hypophthalmichthys molitrix), bighead carp (aristichthys nobilis) and mirror carp (cyprinus carpio) were cultured together in a fish pond at bangladesh agricultural university, mymensingh, in order to determine the food electivity, dietary overlap and food competition among indigenous major carps and exotic carps. phytoplankton (chlorophyceae, ... | 2012 | 24191618 |
| present status and approaches for the sustainable development of community based fish culture in seasonal floodplains of bangladesh. | coordination among the different stakeholders at policy planning, implementation and target beneficiary level, particularly among the agencies responsible for development and management of water resources, agriculture and fisheries, is essential for overall sustainable development. stocking of larger fingerlings at suitable stocking densities of endemic (rohu, catla, mrigal) and exotic (silver carp, bighead carp, common carp/mirror carp) species should be stocked at varying proportion. floodplai ... | 2012 | 24191617 |
| lipid classes and fatty acid profile of selected indian fresh water fishes. | lipid extracts from meat, head and viscera of indian fresh water fishes, viz., catla, rohu, mrigal, common carp and tilapia were analyzed for lipid class distribution and fatty acid profile. the yield of meat ranged from 66.0-79.5% and total lipid content in meat was 0.8-3.8%. the total lipid content was higher (>4.0%) in head and viscera. neutral lipids constituted 71.5-93.3% of the total lipid extract. higher glycolipid content of 25.2% was observed in lipid extract from meat of common carp an ... | 2010 | 23572659 |
| relativein vitro effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown in oocytes of indian major carps,labeo rohita, cirrhinus mrigala andcatla catla. | the relative effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown (gvbd) was investigatedin vitro using folliculated oocytes of three carps,labeo rohita, cirrhinus mrigala, andcatla catla. in all three species progesterone and 17α-hydroxyprogesterone could induce gvbd but relatively 17α,20β-dihydroxyprogesterone was consistently found to be the most potent maturation-inducing steroid. both estradiol-17β and testoste ... | 1989 | 24221598 |
| mercury contamination in some marine biota species from khuzestan shore, persian gulf. | in this study, concentrations of mercury (hg) were analyzed in some marine biota species (fish, shrimp, and crab) from khuzestan shore, north part of the persian gulf. it was also our intention to evaluate potential risks to human health associated with seafood consumption. the results indicated that concentrations of hg in the fish and crustacean were different among the species and tissues. liver in fish and hepatopancreas in crustacean exhibited higher hg concentration than the other tissues. ... | 2016 | 25500758 |
| a comparative study on the physical, chemical and functional properties of carp skin and mammalian gelatins. | carp species forms the bulk of the aquaculture production in india and studies have shown that the filleting waste of these species, particularly skin can be a good source of gelatin. this study is a comparison of the gelatins from these unexploited sources with that of mammalian gelatins to get a better understanding of their physicochemical and functional properties with respect to mammalian gelatins. the study showed that mammalian gelatins had significantly superior physical properties viz., ... | 2014 | 25190867 |
| establishment and characterization of permanent cell line from gill tissue of labeo rohita (hamilton) and its application in gene expression and toxicology. | rohu gill cell line (lrg) was established from gill tissue of indian major carp (labeo rohita), a freshwater fish cultivated in india. the cell line was maintained in leibovitz's l-15 supplemented with 10 % foetal bovine serum (fbs). this cell line has been sub-cultured more than 85 passages over a period of 2 years. the lrg cell line consists of both epithelial and fibroblastic-like cells. the cells were able to grow at a wide range of temperatures from 22 to 32 °c, the optimum temperature bein ... | 2013 | 23224722 |
| development of an es-like cell culture system (resc) from rohu, labeo rohita (ham.). | an embryonic stem (es)-like cell culture system resc from a commercially important freshwater carp, labeo rohita, was developed using blastula stage embryos. the cells were cultured in leibovitz-15 (l-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basic fibroblast growth factor at 28 °c under feeder-free conditions. the es-like cells were characterized by their unique morphology, alkaline phosphatase activity, embryoid body forma ... | 2012 | 22707190 |
| variation in susceptibility pattern of fish to argulus siamensis: do immune responses of host play a role? | branchiuran ectoparasites of the genus argulus can have extensive damaging effects on cultured fish. there exist no systematic studies that evaluate susceptibility or resistance of various carp species to argulus sp. and the underlying mechanisms. the present study aimed at identifying the most susceptible and resistant cultured species, studying settlement and survival of parasite on these species, and finally unravelling the variations of immune response in both resistant and susceptible speci ... | 2016 | 27084477 |
| first record of myxozoan parasites from fresh water fishes of jammu and kashmir and their pathogenecity. | the present study was aimed to isolate myxozoan parasites infecting the native carp schizothorax esocinus (heckel) and pond reared carp labeo rohita (ham.) collected from fresh water ecosystems of jammu and kashmir from july 2013 to june 2014. various organs such as eyes, gills, scales, fins, intestines, visceral organs and pericardial cavity of as many as 100 live specimens were examined. results revealed that 14% of the examined l. rohita were infected with myxobolus rocatlae (ham.) and 6% of ... | 2017 | 28232055 |
| description of a myxozoan parasite myxobolus sp.n pkb2014 from an edible fish, with emphasis on its molecular characterization. | in india, more than 104 species of myxobolus have so far been reported infecting freshwater as well as marine fishes. the study focuses on the description of a new myxosporean species, myxobolus sp.n pkb 2014 from the gill lamellae of an indian major carp labeo rohita. the species have been described on the basis of morphological characterization of the spores, tissue architecture and 18s rdna sequence data. the plasmodia of myxobolus sp.n pkb 2014 were round in shape measuring, 50 to 70 μm in d ... | 2015 | 27844012 |
| molecular characterization of activin receptor type iia and its expression during gonadal maturation and growth stages in rohu carp. | activin receptor type iia (actriia), a transmembrane serine/threonine kinase receptor is an important regulator of physiological traits, viz., reproduction and body growth in vertebrates including teleosts. however, existing knowledge of its role in regulating fish physiology is limited. to address this, we have cloned and characterized the actriia cdna of labeo rohita (rohu), an economically important fish species of the indian subcontinent. comparative expression profiling of the receptor gene ... | 2017 | 27575753 |
| structural and functional characterization of recombinant interleukin-10 from indian major carp labeo rohita. | interleukin-10, an important regulator of both the innate and adaptive immune systems, is a multifunctional major cytokine. though it is one of the major cytokines, il-10 from the indian major carp, labeo rohita, has not yet been characterized. in the present study, we report large scale production and purification of biologically active recombinant il-10 of l. rohita (rlril-10) using a heterologous expression system and its biophysical and functional characterization. high yield (~70 mg/l) of s ... | 2017 | 27689097 |
| enzymatic extraction and characterisation of a thermostable collagen from swim bladder of rohu (labeo rohita). | the fish swim bladder is considered as a potential source of realistic collagen. currently, processing of the indian major carp rohu (labeo rohita) generates an enormous quantity of non-edible by-products, including swim bladders, which are discarded as waste with no commercial value. in the present study, collagen was prepared from rohu swim bladder and its physicochemical and fibril-forming capacities were assessed. | 2017 | 27380938 |
| evaluation of pollution of ganga river water using fish as bioindicator. | ganga river, life line of millions of people got heavily polluted due to uncontrolled anthropogenic activities. to monitor the effect of pollution of the river on its aquatic life, a field study was conducted by analyzing the different biomarker enzymes and biochemical parameters in the various tissues (muscles, liver, gills, kidney, brain, and skin) of the indian major carp labeo rohita collected from the river ganga from different study sites of varanasi district. activities of antioxidant enz ... | 2016 | 27370309 |
| molecular identification of a new myxozoan, myxobolus dermiscalis n. sp. (myxosporea) infecting scales of labeo rohita hamilton in harike wetland, punjab (india). | in the present study, a new species myxobolus dermiscalis n. sp. infecting scales of labeo rohita, an indian major carp from harike wetland in punjab, india has been described on the basis of spore morphology and amplification of a part of 18s rdna gene. the pseudocysts of m. dermiscalis n. sp. are milky white with irregular outline, 0.5-3.6 mm in diameter embedded within the dermal scale in the form of a cavity. the spores 5.84-7.98 × 3.98-5.98 μm in size, having two equal polar capsules 3.98-5 ... | 2016 | 27330981 |
| the first evidence of cholinesterases in skin mucus of carps and its applicability as biomarker of organophosphate exposure. | the presence of cholinesterase (che) activity in skin mucus of three carps, cirrhinus mrigala, labeo rohita, and catla catla and its applicability as biomarker of the organophosphorus insecticide exposure were investigated. biochemical characterization, using specific substrates and inhibitors, indicated that measured esterase activity in skin mucus was mainly owing to ches. significant difference in the proportion of acetylcholinesterase and butyrylcholinesterase activities was observed in skin ... | 2014 | 22887814 |
| description of three new species of the genus myxobolus infecting carp fishes of india. | myxobolus bütschli, 1882 (myxozoa, myxosporea, bivalvulida, myxobolidae) is an important parasitic protozoan of freshwater fishes reported from almost all over the world. the severity of infection may lead to mortality of the host fish. the present paper deals with the description of three new species of myxobolus bütschli, 1882, myxobolus sonarpurensis sp. nov., myxobolus elongatum sp. nov. and myxobolus petalum sp. nov. from a freshwater minor carp labeo bata (hamilton, 1822) and major carp la ... | 2017 | 28316405 |
| role of bacillus licheniformis vs16-derived biosurfactant in mediating immune responses in carp rohu and its application to the food industry. | multifarious applications of bacillus licheniformis vs16-derived biosurfactant were explored. labeo rohita fingerlings were injected intraperitoneally with 0.1 ml of phosphate-buffered saline (pbs) containing purified biosurfactant at 0 (control), 55 (s55), 110 (s110), 220 (s220), or 330 (s330) μg ml(-1) concentrations. various immunological parameters and the expression of immune-related genes were measured at 7, 14, and 21 days post-administration (dpa). at 21 dpa, fish were challenged with ae ... | 2017 | 28400765 |
| construction, de-novo assembly and analysis of transcriptome for identification of reproduction-related genes and pathways from rohu, labeo rohita (hamilton). | rohu is a leading candidate species for freshwater aquaculture in south-east asia. unlike common carp the monsoon breeding habit of rohu restricts its seed production beyond season indicating strong genetic control over spawning. genetic information is limited in this regard. the problem is exacerbated by the lack of genomic-resources. we identified 182 reproduction-related genes previously by sanger-sequencing which were less to address the issue of seasonal spawning behaviour of this important ... | 2015 | 26148098 |
| neuroanatomical localization of nitric oxide synthase (nnos) in the central nervous system of carp, labeo rohita during post-embryonic development. | nitric oxide (no) is a chemically diffusible molecular messenger playing various roles in both vertebrates and invertebrates. nitric oxide synthase (nos) is the key enzyme in synthesis of no. the neuroanatomical distribution pattern of neuronal nitric oxide synthase (nnos) was studied and developing stages of labeo rohita such as hatchlings (10-15mm), frys (15-35mm), semi-fingerlings (35-65mm), fingerlings (65-100mm) and adults (350-370mm) were used. in the telencephalon, nitrergic cells were ob ... | 2015 | 26143285 |
| first evidence of molecular characterization of rohu carp sox2 gene being expressed in proliferating spermatogonial cells. | because little is known about the function of sox2 (sry-related box-2) in teleosts, the objective of this study was to clone and characterize sox2 complementary dna (cdna) from the testis of indian major carp, labeo rohita (rohu). the full-length cdna contained an open reading frame of 936 nucleotides bearing the typical structural features. phylogenetically, sox2 of l rohita was most closely related to freshwater counterparts than marine water. the sequence information of cdna and genomic dna t ... | 2015 | 25913275 |
| bioaccumulation of some heavy metals: analysis and comparison of cyprinus carpio and labeo rohita from sardaryab, khyber pakhtunkhwa. | we examined and compared heavy metals bioaccumulation in cyprinus carpio and labeo rohita netted from sardaryab, a tributary of river kabul. by using atomic absorption spectrometry we assessed different organs including livers, gills, and muscles. metals studied were chromium, iron, zinc, lead, and copper. livers of both species showed higher concentrations of metals while muscles showed the least amount. chromium and iron were the highly concentrated metals in the gills and livers of both speci ... | 2017 | 28396869 |
| sodium cyanide induced alteration in the whole animal oxygen consumption and behavioural pattern of freshwater fish labeo rohita. | sodium cyanide is a common environmental pollutant which is mainly used in many industries such as mining, electroplating, steel manufacturing, pharmaceutical production and other specialized applications including dyes and agricultural products. it enters aquatic environment through effluents from these industries. static renewal bioassay test has been conducted to determine lc, of sodium cyanide on indigenous freshwater carp, labeo rohita. the behavioural pattern and oxygen consumption were ob ... | 2015 | 25895263 |
| the complete mitochondrial genome sequence of cirrhinus mrigala (♀) × labeo rohita (♂). | we cloned and sequenced the complete mitochondrial dna (mtdna) of cirrhinus mrigala (♀) × labeo rohita (♂) by pcr-based method. the total of the mtdna was 16,595 bp. it consisted of 13 protein-coding genes, 22 trnas, 2 rrna genes, and one putative control region (d-loop region). the overall base composition on strand was as follows a: 32.09%, g: 15.51%, c: 27.95%, t: 24.45%, and the a + t content 56.54%. all the protein-coding genes initiated by typical atg codon, and ten genes ended with the co ... | 2016 | 25350736 |
| preliminary genetic linkage map of indian major carp, labeo rohita (hamilton 1822) based on microsatellite markers. | linkage map with wide marker coverage is an essential resource for genetic improvement study for any species. sex-averaged genetic linkage map of labeo rohita, popularly known as 'rohu', widely cultured in the indian subcontinent, was developed by placing 68 microsatellite markers generated by a simplified method. the parents and their f1 progeny (92 individuals) were used as segregating populations. the genetic linkage map spans a sex-averaged total length of 1462.2 cm, in 25 linkage groups. th ... | 2015 | 26174674 |
| the β-actin gene promoter of rohu carp (labeo rohita) drives reporter gene expressions in transgenic rohu and various cell lines, including spermatogonial stem cells. | we previously characterized the β-actin gene promoter of indian domesticated rohu carp (labeo rohita) and made a reporter construct via fusion to green fluorescence protein (gfp) cdna. in this study, the same construct was used to breed transgenic rohu fish. about 20% of the transgenic offspring showed ubiquitous expression of the reporter gfp gene. in a few of the transgenic fish, we documented massive epithelial and/or muscular expression with visible green color under normal light. the expres ... | 2015 | 26204405 |
| immunomodulatory effect of arsenic on cytokine and hsp gene expression in labeo rohita fingerlings. | immune system is fundamental for survival of an organism against invading pathogens and other harmful agents. cytokines, the signaling proteins that are produced transiently after cell activation and exert pleiotropic effects on cells of the immune system, are important mediators of cell mediated immune response. when expressed in a dysregulated fashion cytokines can underlie either immunodeficient or immunopathologic states. heat shock proteins (stress proteins, hsps) are also key proteins, whi ... | 2015 | 25652292 |
| examining pyrethroids, carbamates and neonicotenoids in fish, water and sediments from the indus river for potential health risks. | this 3 × 3 factorial study assessed pyrethroids, carbamates and neonicotenoids groups of pesticides in replicated samples of three fish species from low (s1, reference), medium (s2) and heavy (s3) polluted sites receiving agricultural run-offs around the indus river. water and sediment samples from the same sites were also analysed for these pesticides by using high-performance liquid chromatography. out of nine investigated pesticides, only three pesticides (deltamethrin, carbofuran and cyperme ... | 2015 | 25632902 |
| population structure and genetic diversity of indian major carp, labeo rohita (hamilton, 1822) from three phylo-geographically isolated riverine ecosystems of india as revealed by mtdna cytochrome b region sequences. | the population structure and genetic diversity of rohu (labeo rohita hamilton, 1822) was studied by analysis of the partial sequences of mitochondrial dna cytochrome b region. we examined 133 samples collected from six locations in three geographically isolated rivers of india. analysis of 11 haplotypes showed low haplotype diversity (0.00150), nucleotide diversity (π) (0.02884) and low heterogeneity value (0.00374). analysis of molecular variance (amova) revealed the genetic diversity of l. roh ... | 2016 | 28024428 |
| first report on fish cysteine as a biomarker of contamination in the river chenab, pakistan. | the eastern and southern parts of the faisalabad city produce considerable quantities of industrial and municipal pollutants, much of which is drained into the river chenab, reducing the productivity of fauna and flora in the river. this study was aimed to determine whether cysteine is useful as a biomarker of exposure to polluted fresh water. the amino acid profile of fish muscle was analyzed by paper chromatography in cirrhinus mrigala and labeo rohita from the river chenab to determine habita ... | 2016 | 27117257 |
| fishmicrosat: a microsatellite database of commercially important fishes and shellfishes of the indian subcontinent. | microsatellite dna is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. the biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. the microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. the system also helps to design primer sequences for flank ... | 2013 | 24047532 |
| identification and characterization of maturation-promoting factor from catfish,clarias batrachus. | maturation-promoting factor (mpf) extracted from maturing oocytes of catfishes (clarias batrachus andheteropneustes fossilis) and carp (labeo rohita) induces 100% germinal vesicle breakdown (gvbd) when microinjected intoclarias immature unstimulated oocytes. the presence of a similar mpf activity has also been demonstrated in the active fractions collected after superose 12. sds-page analyses of cytosolic extracts (ce) prepared from immature and mature oocytes revealed the presence of 34- and 46 ... | 1996 | 24194144 |
| establishing targeted carp tlr22 gene disruption via homologous recombination using crispr/cas9. | recent advances in gene editing techniques have not been exploited in farmed fishes. we established a gene targeting technique, using the crispr/cas9 system in labeo rohita, a farmed carp (known as rohu). we demonstrated that donor dna was integrated via homologous recombination (hr) at the site of targeted double-stranded nicks created by crispr/cas9 nuclease. this resulted in the successful disruption of rohu toll-like receptor 22 (tlr22) gene, involved in innate immunity and exclusively prese ... | 2016 | 27079451 |
| population genetic characterization and family reconstruction in brood bank collections of the indian major carp labeo rohita (cyprinidae:cypriniformes). | the founder stock of a captive breeding program is prone to changes in genetic structure due to inbreeding and genetic drift. genetic characterization of the founder population using suitable molecular markers may help monitor periodic changes in the genetic structure in future. to develop benchmark information about the genetic structure we analyzed six microsatellite loci in the brodbank collections of rohu (labeo rohita) originated from three major rivers-the jamuna, the padma and the halda. ... | 2015 | 26697284 |
| myxobolus nanokiensis sp. nov. (myxozoa: bivalvulidae), a new pathogenic myxosporean parasite causing haemorrhagic gill disease in cultured indian major carp fish, labeo rohita (hamilton 1822) in punjab, india. | the plasmodia of myxobolus nanokiensis sp. nov. were found infecting gills of labeo rohita (hamilton 1822) the infection rate was found to be 36.67 % (30 fishes were examined and 11 fishes were infected) in the nanoki pond in patiala district punjab. numerous minute plasmodia each filled with 150-200 spores were detected. smear of scrapped blood-tinged mucous from gills exhibited millions of spores. histological sections also indicated numerous plasmodia measuring 38.33-40.33 μm in diameter in t ... | 2015 | 26345042 |
| prostanoids-induced dispersion in the melanophores of a carp labeo rohita (ham.). | effects of three prostaglandins (i.e., prostanoids) and one of its precursors, arachidonic acid, were examined on the melanophores of the fish labeo rohita (ham.). pge₁, pge₂, pgf(2α) and arachidonic acid elicit a concentration-related dispersion in the fish melanophores. in vitro analysis of melanophores was performed through incubation of the isolated fish scales in different agonists and antagonists solutions. dispersal effect of prostanoids may be mediated directly through the typical recept ... | 2014 | 23801365 |
| identification of reproduction-related genes and ssr-markers through expressed sequence tags analysis of a monsoon breeding carp rohu, labeo rohita (hamilton). | labeo rohita (ham.) also called rohu is the most important freshwater aquaculture species on the indian sub continent. monsoon dependent breeding restricts its seed production beyond season indicating a strong genetic control about which very limited information is available. additionally, few genomic resources are publicly available for this species. here we sought to identify reproduction-relevant genes from normalized cdna libraries of the brain-pituitary-gonad-liver (bpgl-axis) tissues of ad ... | 2013 | 23583682 |
| contamination of the river ganga and its toxic implication in the blood parameters of the major carp labeo rohita (ham). | a field study was conducted to examine different physicochemical properties of water and various haematological and biochemical parametres of the fish labeo rohita collected from the ganga river (national river of india) at varanasi district, india. the water was found to be greatly contaminated with a number of dissolved metals (fe, cr, zn, cu, mn, ni and pb) whose concentrations were above the safe limits suggested by bureau of indian standard (bis 1991) for drinking water (fe, 1,353.33 %; cr, ... | 2013 | 23456946 |
| gene structure and identification of minimal promoter of pou2 expressed in spermatogonial cells of rohu carp, labeo rohita. | mammalian pou5f1 is a known transcriptional regulator involving maintenance of embryonic and spermatogonial stem cells. little is known about teleost pou2, an ortholog of mammalian pou5f1. evidences of discrepancy in expression pattern between fish species were documented. to better understand, we have cloned and characterized pou2 gene of farmed rohu carp, labeo rohita. it contained five exons with an open reading frame of 1419 bp long, translatable to 472 aa. a bipartite dna binding domain ter ... | 2014 | 24566687 |
| evaluation of trace metal content by icp-ms using closed vessel microwave digestion in fresh water fish. | the objective of the present study was to investigate trace metal levels of different varieties of fresh water fish using inductively coupled plasma mass spectrophotometer after microwave digestion (md-icpms). fish samples were collected from the outlets of twin cities of hyderabad and secunderabad. the trace metal content in different varieties of analyzed fish were ranged from 0.24 to 1.68 mg/kg for chromium in cyprinus carpio and masto symbollon, 0.20 to 7.52 mg/kg for manganese in labeo rohi ... | 2014 | 24744789 |
| assessment of genotoxic and mutagenic potential of hexavalent chromium in the freshwater fish labeo rohita (hamilton, 1822). | the present study was undertaken to investigate the genotoxicity and mutagenicity of sublethal concentrations of hexavalent chromium (potassium dichromate) in the indian major carp, labeo rohita. the 96 h lc50 value of potassium dichromate estimated was 118 mg l(-1) by probit analysis using spss (version 16.0) software. based on 96 h lc50 value, three sublethal test concentrations of potassium dichromate (29.5, 59.0 and 88.5 mg l(-)(1)) were selected and specimens were exposed in vivo to these t ... | 2015 | 24649973 |
| efficacy of two avermectins, doramectin and ivermectin against argulus siamensis infestation in indian major carp, labeo rohita. | the efficacy of two avermectins, doramectin and ivermectin via oral and parenteral routes and their prophylactic effects against argulus siamensis infestation in indian major carp, labeo rohita was studied. doramectin and ivermectin were fed once to l. rohita sub-adults naturally infested with a. siamensis parasites at graded levels of 1000, 750 and 500 μg/kg body weight and 500, 300 and 200 μg/kg body weight, respectively. the mean time of clearance of parasites was 24 ± 0.149 h for 1000 and 24 ... | 2012 | 22673107 |
| impact of cypermethrin on fingerlings of common edible carp (labeo rohita). | laboratory evaluations were made to assess the toxicological and biochemical effect of cypermethrin on fingerlings of common edible freshwater culture carp (labeo rohita). there was a significant negative (p < 0.05) correlation observed between effective doses of cypermethrin and exposure periods; that is, lc₅₀ values decreased from 0.323 μg/l (6 h) to > 0.278 μg/l (12 h), > 0.240 μg/l (18 h) and >0.205 μg/l (24 h). exposure to sublethal doses of cypermethrin for 24 h and 96 h exposure period ... | 2012 | 22666103 |
| response of the chromatophores in relation to the healing of skin wounds in an indian major carp, labeo rohita (hamilton). | chromatophores show significant changes during healing of skin wounds in labeo rohita (common name--rohu). wound area can be divided into regions i, ii and iii. after infliction of wound, skin colour becomes significantly dark by 2h that is gradually restored by 2d. in regions ii and iii at 5 min, epidermal melanophores appear with beaded dendrites. in these regions at 2h and in region i at 6h, epidermal melanophores appear small, rounded or irregular shaped having dendritic processes with aggre ... | 2012 | 22321648 |
| histochemical analysis of glycoproteins in the secretory cells in the epidermis of the head skin of indian major carp, labeo rohita. | a series of histochemical procedures were employed to localise and characterise glycoprotein (gp) classes produced by the epithelial cells, the type a and the type b mucous goblet cells (mgcs) and the club cells in the epidermis of labeo rohita. the epithelial cells secreted gps with oxidizable vicinal diols and gps with sialic acid residues without o-acyl substitution in low concentrations. the type a mgcs and the type b mgcs, in contrast, produced these gps in high concentrations. further, the ... | 2012 | 22939776 |
| identification of promoter within the first intron of plzf gene expressed in carp spermatogonial stem cells. | promyelocytic leukemia zinc finger (plzf), a transcriptional repressor, is involved in survival and maintenance of pluripotent stem cells including embryonic and spermatogonial stem cells in mammals. its cdna was characterized and expression in proliferating spermatogonial stem cells of rohu (labeo rohita), a farmed carp, was documented. in teleost, the information on its promoter activity is lacking. here, we have isolated, sequenced and performed the first characterization of regulatory elemen ... | 2014 | 24990695 |
| gnih and gnrh expressions in the central nervous system and pituitary of indian major carp, labeo rohita during ontogeny: an immunocytochemical study. | gonadotropin-releasing hormone (gnrh) is the major hypothalamic neuropeptide stimulating gonadotropin secretion in vertebrates. in 2000, gonadotropin-inhibitory hormone (gnih) was discovered as a hypothalamic neuropeptide that inhibits gonadotropin secretion in birds. subsequent studies have shown that gnih is present in the brain of other vertebrates. we show for the first time gnih immunoreactivity in the central nervous system and pituitary during development of indian major carp, labeo rohit ... | 2015 | 24955881 |
| innate immune response of indian major carp, labeo rohita infected with oomycete pathogen aphanomyces invadans. | the fish pathogenic oomycete aphanomyces invadans is the causative agent of epizootic ulcerative syndrome (eus), a fish disease of international significance and reportable to the world organisation for animal health. in spite of the current and potential impact of a. invadans infection on fisheries and aquaculture sectors of the world, very little is known about the host-a. invadans interactions. in the present study, following experimental infection with a. invadans in one of the indian major ... | 2014 | 24945572 |
| molecular characterization and expression of type-i interferon gene in labeo rohita. | genes coding for type-i interferon (i-ifn) has been cloned from labeo rohita, a commercially important and widely cultured fish in india and south east asia. in the present study, full-length gene of i-ifn was amplified and sequenced. the sequence analysis revealed that i-ifn consists of 1,786 bp genomic sequence with four introns and five exons and an orf of 546 bp encoding for a putative protein of 181 amino acids. the mature protein has a molecular weight of 18.97 kda and consists of 158 amin ... | 2014 | 24449367 |
| age and growth in the indian major carp labeo rohita (cypriniformes: cyprinidae) from tropical rivers of ganga basin, india. | labeo rohita is a member of the indian major carp species and originally an inhabitant of the ganga river network in india. it is among the top ten aquaculture species of the world. since there is a lack of information on the growth pattern of the wild populations of this species, this study aimed at evaluating the pattern of age and growth, to support the development of effective management plans. a total of 1082 samples of l. rohita were obtained from may 2009 to july 2012 in six drainages of ... | 2013 | 24432546 |
| hematological, biochemical and enzymological responses in an indian major carp labeo rohita induced by sublethal concentration of waterborne selenite exposure. | selenium (se) pollution in aquatic ecosystem is an environmental issue throughout the world. elevated concentrations of inorganic se from agricultural and industrial processes may cause adverse biological effects in aquatic organisms such as fish. in the present study, labeo rohita an indian major carp were exposed to sublethal concentration of se (sodium selenite) for 35days and certain hematological, biochemical and enzymological parameters were estimated. the median lethal concentration of wa ... | 2014 | 24183823 |
| molecular cloning and characterization of toll-like receptor 3, and inductive expression analysis of type i ifn, mx and pro-inflammatory cytokines in the indian carp, rohu (labeo rohita). | toll-like receptors (tlrs) are one of the key components of innate or non-specific immunity. among various types of tlrs, tlr3 recognizes dsrna, the genetic material or replicative intermediate of many rna viruses and triggers tir-domain-containing adapter-inducing interferon-β dependent signalling pathway to induce type i interferon (ifn) and pro-inflammatory cytokines. in this study, we cloned and characterized full-length tlr3 cdna in rohu (labeo rohita), that comprised of 2,619 bp nucleotide ... | 2013 | 23065215 |
| histopathological effects and bioaccumulation of mercury in the kidney of an indian major carp, labeo rohita (hamilton). | the effect of mercuric chloride on the histo-morphology and bioaccumulation in the kidney of an indian major carp, labeo rohita (hamilton) were examined after exposing the fish (15-20 cm) to three sublethal concentrations (0.033, 0.066 and 0.132 mg/l) of hgcl(2) for 30 days. mercury deposition in kidney tissues had increased significantly with dose and exposure duration dependant manner. several histological changes were noted in the kidney of all treated groups in compare to control group. | 2012 | 22810019 |
| toxicological effects of the antibiotic oxytetracycline to an indian major carp labeo rohita. | oxytetracycline (otc), an antibacterial agent, is extensively used in aquaculture practices all over the world. despite its use, the toxicity of otc to freshwater fish has been scarcely investigated. in this study, labeo rohita were exposed to different concentrations (20, 40, 60, 80, 100, and 120 mg l(-1)) of otc. based on the survival-to-mortality ratio, an 80 mg l(-1) concentration was selected for sublethal toxicity analysis. fish were exposed to the above-mentioned concentration for a perio ... | 2013 | 23192588 |
| characterization of vibrio species isolated from freshwater fishes by ribotyping. | three vibrio species from the resident microflora of gastrointestinal tract of freshwater carps and prawns were isolated and confirmed biochemically as v. fluvialis from cyprinus carpio/labeo rohita; v. parahaemolyticus from macrobrachium rosenbergii and v. harveyi from macrobrachium malcomsoni. the genetic relationship among these vibrio species was carried out by polymerase chain reaction (pcr) amplification of 16s rrna gene followed by restriction digestion with hae iii, bam hi and pst i. den ... | 2010 | 23100815 |
| morphotaxonomical description of three new species of myxobolus bütschli, 1882 (myxozoa: myxosporea: bivalvulida) from edible freshwater fishes of west bengal, india. | three new species of myxobolus are described from the mucous membrane around gill lamellae of carps of west bengal. the new species have been named as m. muralidharani sp. n., m. nilimae sp. n. from labeo rohita, and m. harpreetae sp. n. from labeo bata respectively. spores of m. muralidharani sp. n. measured 13.84 ± 0.049 (13-14) × 4.95-6 (5.35) µm, with two elongated equal sized polar capsules. spores of m. nilimae sp. n. measured 15.9-17.1 (16.65) µm × 6.2-7.5 (6.9) µm, with two equal petal s ... | 2017 | 28316395 |
| myxobolus chushi n. sp. (myxozoa:myxosporea) parasitizing schizothorax niger (heckel), a native cyprinid fish from wullar lake in kashmir himalayas. | in the study, a new species, myxobolus chushi n. sp. infecting gills of wild specimens of schizothorax niger (heckel) inhabiting wullar lake in kashmir himalayas, (j&k) india has been described based on morphology of the myxospore and using partial 18s rdna sequencing. pathological changes in the gills have been studied with the help of histological sections stained with luna's method. twenty fish specimens were examined, out of which four had oval, white plasmodia in gills measuring 2.0×0.5mm. ... | 2017 | 28232063 |
| effect of dactylogyrus catlaius (jain 1961) infection in labeo rohita (hamilton 1822): innate immune responses and expression profile of some immune related genes. | the monogenean ectoparasite, dactylogyrus sp. is a major pathogen in freshwater aquaculture. the immune responses in parasitized fish were analyzed by quantitation of innate immune factors (natural agglutinin level, haemolysin titre, antiprotease, lysozyme and myeloperoxidase activities) in serum and immune-relevant gene expression in gill and anterior kidney. the antiprotease activity and natural agglutinin level were found to be significantly higher and lysozyme activity was significantly lowe ... | 2014 | 24669670 |